The study of energy in living systems (environments) and the organisms (plants and animals) that utilize them.
I'm a st.Xavier's student . i think this ppt will be helpful to the others. Because this is needed in our daily life.
Formation and fate of Ammonia
Transdeamination, oxidative and non oxidative deamination, Ammonia transport, Ammonia intoxication, Ammonia detoxification
The study of energy in living systems (environments) and the organisms (plants and animals) that utilize them.
I'm a st.Xavier's student . i think this ppt will be helpful to the others. Because this is needed in our daily life.
Formation and fate of Ammonia
Transdeamination, oxidative and non oxidative deamination, Ammonia transport, Ammonia intoxication, Ammonia detoxification
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
2. ATP (Adenosine Triphosphate) – the major source of energy in cell metabolism.
- chemical energy converted from “electron energy”
The Function of ATP
transport work moving substances across cell membranes.
used for mechanical work, supplying the energy needed for muscle
contraction.
supplies energy not only to heart muscle (for blood circulation) and skeletal
muscle (such as for gross body movement), but also to the chromosomes and
flagella to enable them to carry out their many functions.
A major role of ATP is in chemical work, supplying the needed energy to
synthesize the multi-thousands of types of macromolecules that the cell needs
to exist.
used as an on-off switch both to control chemical reactions and to send
messages.
3. Plants use the energy in ATP molecules to move water, repair themselves, and
grow.
Plants vital energy conversions:
1. Light energy Electron energy
2. Electron energy Chemical energy (ATP
3. Chemical energy (ATP) sugars
Respiration - the process in which nutrients are converted into useful energy in a
cell
4. Photosynthesis
- The entire process of trapping light energy into the bonds of a sugar
molecule.
Two processes involves:
1. Light-dependent Phase – solar energy is trapped and used to
excite electrons to higher energy levels. These electrons are then
passed down an electron transport chain, and the energy released is
used to make ATP. After the electrons have completed their passage
down the electron transport chain, they are passed to a second
photosystem (known as photosystem I) and more light energy is
harvested. Once the electrons have been given this energy, they are
passed to molecule known as NADP (that then becomes NADPH) and
held there ready to be used in the next phase of photosynthesis.
5. NAD - Nicotinamide adenine dinucleotide - is an important coenzyme found
in cells. It plays key roles as a carrier of electrons and a participant in metabolic
redox reactions, as well as in cell signaling.
Nicotinamide adenine dinucleotide phosphate (NADP) - is the reduced form
of NAD. The main role of NADP in metabolism is the transfer of electrons from one
redox reaction to another, which is very important in the release of energy from
nutrients.
6. Nicotinamide adenine dinucleotide phosphate-oxidase (NADPH) - is the
reduced form of NADP.
The NADPH oxidase complex is a cluster of proteins that donate an electron
from NADPH to molecular oxygen (O2) to produce superoxide (O2-). This initiates
the respiratory burst, a key step in immune defense against bacterial and fungal
pathogens. The importance of this process to human health is manifested in
chronic granulomatous disease (CGD), which refers to any of several hereditary
diseases in which certain oxidase proteins are defective.
An important role for NADPH oxidase in phagocytes is bacterial killing, which
occurs in phagosomes.
7. Chloroplasts – an organelle that converts sunlight, carbon dioxide, and water
into sugars in the process of photosynthesis.
Photosystem II – embedded in the thylakoid membranes of chloroplasts,
special solar collectors composed of pigment molecules, the major ones of which
are chlorophyll a and chlorophyll b.
Photolysis (“splitting by light”) – a process when the solar collector
loses two of its electrons, they are replaced by electrons from an enzyme. The
enzyme recovers its electrons by splitting a water molecule.
Hydrogen ions are formed and oxygen gas is given off as a waste product.
2H2O 4H+ + 4e + O2 or
Photosystem I – second solar collector
8. 2. The Light-independent Phase – short-term chemical and electron
energy in the ATP and NADPH molecules are converted into long-term chemical
energy in the covalent bonds of sugar molecules. The electrons are moved away
from oxygen atoms and toward the carbon and hydrogen atoms. This is a
nonspontaneous reaction requiring an input of energy that is then stored for later
use by the cell.
- Light-independent reactions of photosynthesis or dark reaction of
photosynthesis
Photosynthesis:
CO2 + 2H2O CH2O + H2O + O2
-
9. Cellular Respiration
- the most efficient way for cells to harvest energy stored in food.
Respiration :
C6H12O6 + O2 CO2 +H2O + Energy
glucose oxygen carbon water
dioxide
10. Aerobic Respiration
- A vast majority of organisms break down sugar molecules all the way to
carbon dioxide and water.
Three major stages:
1. Glycolysis – glucose molecules (from starch or sucrose) are broken down into
smaller 3-carbon units called pyruvate and some of the energy released is
trapped as ATP and NADH.
- Does not require oxygen
2. Krebs Cycle – the pieces of the glucose molecule left after glycolysis are
broken all the way down to carbon dioxide. GTP (a nucleotide similar to ATP),
NADH, and other high-energy molecules are formed.
GTP - Guanosine triphosphate
11. 3. ATP Synthesis – NADH molecules formed in glycolysis and Krebs cycle are
converted back to NAD molecules so that they be reused.
12. Anaerobic Respiration
- Some organisms that do not require oxygen to regenerate.
- Organisms depend on glycolysis to generate ATP and on fermentation to
regenerate NAD. A byproduct of this reaction is ethanol.
Fermentation is a metabolic process that converts sugar to acids, gases and/or
alcohol.
Pyruvate + NADH Ethanol + CO2 + NAD
13. Respiration and Photosynthesis
Compared
Photosynthesis
1. Occurs only in the chlorophyll-
bearing cells of plants
2. Needs the presence of light
3. Water and carbon dioxide are used
4. oxygen is given off as a waste
product
5. Food is built or synthesized
6. The weight of the plant is increased.
7. Energy is stored
Respiration
1. Occurs in every living plant and
animal cell
2. Occurs at all times
3. Water and carbon dioxide are given
off as waste products
4. Oxygen is used in the process
5. Food is destroyed to release its
energy.
6. The weight of the plant is decreased
7. Energy is released.