Effect of Rhizobial Inoculation with Indigenous and Commercial Strains on Nodulation, Biomass and Nitrogen Fixation of Cowpea Genotypes in Northern Guinea Savanna of Nigeria

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International Institute of Tropical Agriculture

Indigenous elite strain of cowpea rhizobia. Evaluation of their symbiotic effectiveness in Biological Nitrogen Fixation (BNF)

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Effect of Rhizobial Inoculation with Indigenous and
Commercial Strains on Nodulation, Biomass and
Nitrogen Fixation of Cowpea Genotypes in
Northern Guinea Savanna of Nigeria
By
Muhammad N. Isa1,2
, A. A. Yusuf1
N. Kamai2
and M. Dianda2
1
Department of Soil Science, Faculty of Agriculture, Ahmadu Bello University, Zaria.
2
International Institute of Tropical Agriculture, Ibadan
1

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INTRODUCTION
 Cowpea (Vigna unguiculata (L) Walp) is an important
vegetable and grain legume crop
 Its grain and haulm are highly valued for food and
forage
 Biological nitrogen fixation (BNF) is a cheap and
environmentally friendly source of N for plant
 High N2 fixation requires the presence of adequate
numbers of highly effective rhizobia in the soil
2

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OBJECTIVES
 To identify indigenous elite strain of cowpea
rhizobia
 To evaluate their symbiotic effectiveness in
Biological Nitrogen Fixation (BNF)
3

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MATERIALS AND METHODS
 Experimental Site
• Screen house of the IAR/ ABU, Zaria.
 Soil Sampling
• Bulk soil sample collected from IAR research field
was used for the experiments.
4

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MATERIALS AND METHODS
CONT.
 Screenhouse Experiment
• Five indigenous rhizobia strains SAMFIX 605,
SAMFIX 618, SAMFIX 624, SAMFIX 659 and
SAMFIX 679.
• Two commercial strains BR 3262 and BR 3267
• Two controls (uninoculated and with nitrogen
only)
• A reference crop (maize [SAMMAZ-14])
5

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MATERIALS AND METHODS
CONT
• The experiment was carried out on four (4)
genotypes of cowpea
I. UAM09 1051-1
II.UAM09 1055-6
III.IT99K-573-1-1
IV.IT89KD-288
• The pots were arranged in a randomized
complete block design (RCBD) with three
replications
6

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MATERIALS AND METHODS
CONT
 Root nodules and plant
shoot were sampled at
eight weeks after
sowing (WAS) for.
• Nodulation
• Biomass assessment
• nitrogen uptake
• biological nitrogen
fixation
7

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RESULT
Interaction between cowpea genotypes and rhizobium strain
on nodule dry weight
8

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RESULT
Interaction between cowpea genotypes and rhizobium
strain on shoot dry weight
9
SE=0.960

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RESULT
Interaction between cowpea genotypes and rhizobium strain on
nitrogen uptake
10

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RESULT
Interaction between cowpea genotypes and rhizobium strain on
nitrogen fixation
11

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CONCLUSION
 The indigenous strain gave higher
• nodule dry weight with UAM09 1051-1 and
• shoot dry weight with IT89KD-288
 N uptake and BNF was highest with the
commercial strain with IT89KD-288
12

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Acknowledgement
 The authors express their sincere appreciation to
N2Africa/IITA for their support.
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International Institute of Tropical Agriculture

(1) IITA is the International Institute of Tropical Agriculture, established in 1967 and headquartered in Ibadan, Nigeria with 21 stations in 30 countries. (2) IITA adopted DataCite DOIs in 2017 through the British Library Consortium to create a trusted institutional data repository meeting FAIR data principles. (3) IITA mints DOIs through an automatic Python script integrating with its Cassavabase database, and manually through its Fabrica portal, to increase data visibility, citation, and improve data management practices.

Induction of early flowering in cassava through light supplementation and CM...

International Institute of Tropical Agriculture

This document summarizes Samwel Muiruri Kariuki's research at IITA Kenya on inducing early flowering in cassava. The document discusses two methods: using LED light supplementation to induce flowering within 4 months, compared to 10 months without light; and developing a CMV-inducible CRISPR-Cas9 system to edit cassava genes in a virus-activated manner. Preliminary results show light supplementation significantly increased the number of flowering plants compared to the control. The researcher is working to assemble constructs using a CMV promoter to drive Cas9 expression and test them in Nicotiana benthamiana transformations. The goal is to create a virus-inducible gene editing system for cassava.

Producing yam mother plants to collect vines for propagation

International Institute of Tropical Agriculture

Effects of moult and breeding on the body condition of some forest birds in s...

International Institute of Tropical Agriculture

Conserving Nigeria’s rarest endemic bird: Ibadan Malimbe, Malimbusibadanensis

International Institute of Tropical Agriculture

The document discusses conserving the Ibadan Malimbe, an endemic bird species found only in Nigeria that is endangered. It describes the International Institute of Tropical Agriculture's (IITA) efforts to conserve the species, which include hosting an Important Bird Area, monitoring the bird population, restoring habitat, and raising awareness. IITA's research has found declining numbers of Ibadan Malimbe due to forest isolation, clearance for agriculture and development, competition with other species, and increased nest destruction, threatening the estimated 2,500 remaining individuals.

Cassava brown streak epidemiology in Eastern Democratic Republic of the Congo

International Institute of Tropical Agriculture

Assessment of genetic diversity among Rwandan cassava (Manihot esculenta) ger...

International Institute of Tropical Agriculture

9 osunbade identification of end users preferences of a cassava product

International Institute of Tropical Agriculture

This document summarizes a study on identifying the preferences of cassava product ("gari") end users in Benue State, Nigeria. The study found that farmers preferred cassava varieties with heavy, long roots that are not rotten or woody, while processors preferred varieties with white, dry peeled roots and less water in the mash. Marketers and consumers preferred gari that is shiny, dry, heavy, sweet with no lumps or smooth and white in color. The preferences identified will help breeders develop new cassava varieties that meet the needs of all end users.

7 helen ufondu perception of yam landraces quality among value chain actors i...

International Institute of Tropical Agriculture

The document discusses a study on the perception of quality in yam landraces among value chain actors in yam producing areas of Nigeria. It finds that Faketsa, Igum, Opoko, and Ushu are the most commonly cultivated varieties for pounded yam and yam flour production. Yam flour is typically processed from fresh yams through washing, peeling, cutting, drying, and grinding. Smoothness and mouldability are key factors in accepting pounded yam and yam fufu. The study recommends further research on Faketsa's qualities for pounded yam and yam flour to aid variety selection for these products.

8 kazeem quality attributes and consumer acceptability of cookies flavoured

International Institute of Tropical Agriculture

1. The study evaluated the quality attributes of cookies flavored with Aidan (Tetrapleura tetraptera) as a substitute for vanilla. 2. Results showed that increasing the substitution level of Aidan for vanilla increased proximate nutrients but decreased carbohydrates and energy. 3. Cookies with 75% Aidan substitution had similar taste and crispness to the 100% vanilla cookie but were most acceptable overall to consumers.

6 anajekwu ekpereka chemical, functional and pasting properties of flours pro...

International Institute of Tropical Agriculture

This document reports on a study that analyzed the chemical, functional, and pasting properties of flours produced from four varieties of unripe plantain. The objectives were to determine the chemical composition, functional properties, pasting properties, and color parameters of the different plantain flours. Materials and methods included obtaining four varieties of plantain, producing the flours using various processing steps, and analyzing the flours for moisture, ash, protein, fat, fiber, starch, sugar, minerals, functional properties using various tests, pasting properties using a rapid visco analyzer, and color parameters. The results showed differences between varieties in the measured properties, with some varieties having higher nutritional or functional qualities. The conclusions were that the

4 ayodele adenitan survey of dried plantain (musa paradisiaca) chips processo...

International Institute of Tropical Agriculture

This document summarizes a survey of dried plantain chip processors in Ondo State, Nigeria. It describes the background and methods used in the survey. Key findings include that most processors are women between the ages of 25-40 who view chip processing as difficult work. Common challenges included the time-consuming nature, pest infestation during storage, and weather issues during drying. The conclusion recommends addressing animal contamination during drying and limiting the use of toxic preservatives to improve product quality and safety.

2 akin olagunju does crop diversification influenc e food and nutrition secur...

International Institute of Tropical Agriculture

The document examines the effect of crop diversification on food and nutrition security among smallholder farming households in Nigeria. It analyzes data from the 2015 Nigerian General Household Survey on 2,041 households. It finds that crop diversification has a positive impact on dietary diversity, increasing it by 10.9%, but negatively impacts subjective food security. However, households with greater crop diversification had a 53.8% higher likelihood of being food secure. The study thus concludes that while crop diversification improves nutrition, broader support is still needed for farming households to ensure food security.

3 akinsola carotenoid apparent retention in ogi flour made from different pro...

International Institute of Tropical Agriculture

The document summarizes a study on the apparent retention of carotenoids in ogi flour made from different provitamin A maize genotypes. It finds that PVA SYN HGBC0 showed the highest carotenoid and provitamin A retention after processing ogi flour, making it the best genotype studied for producing nutritious ogi. The study aims to establish how processing affects carotenoid levels in ogi, an important food in Nigeria, to reduce micronutrient deficiencies in children.

1 pearl amadi assessing the level of consumption of pro vitamin a cassava pr...

International Institute of Tropical Agriculture

The document assessed the level of consumption of pro-vitamin A cassava products among rural households in Nigeria. It found low levels of consumption of products like tapioca, flakes, and vitamin-fortified baked goods. Consumption varied by state, with Akwa Ibom having the highest levels. It recommends increasing production of value-added products and nutritional education campaigns to boost consumption and reduce vitamin A deficiency.

Prof janice olawoye

International Institute of Tropical Agriculture

Professor Janice Olawoye had a 38-year career as a Professor of Rural Sociology at the University of Ibadan, where she served in various administrative roles including Head of Department and Dean. She supervised 30 PhDs and many other students and published over 70 papers. Professor Olawoye also consulted for international development organizations and worked with IITA on workshops, advisory boards, and fellowship programs. She is married with four sons and six grandchildren.

Inqaba biotech presentation

International Institute of Tropical Agriculture

inqaba Biotec is Africa's leading genomics company that aims to catalyze Africa's prosperity through genomics. It offers core services including oligonucleotide synthesis, DNA sequencing using ABI3130XL, ABI3500XL, and Illumina MiSeq platforms, SNP genotyping, bioinformatics, and molecular diagnostic solutions. inqaba Biotec works to address challenges African researchers face regarding logistics, technical know-how, cost, and support through its partnerships and local services. Its vision is to remain a leading genomics company in Africa.

Iarsaf symposium adaptation to climate change

International Institute of Tropical Agriculture

Janice E. Olawoye presented on adaptation to climate change and indigenous and formal mitigation strategies. She discussed how climate change negatively impacts people through changes in weather patterns, threats to food security and health. Indigenous communities have adapted through practices like multiple cropping and migration. However, increased frequency and intensity of climate events requires more formal strategies like afforestation, drought-resistant crops, and early warning systems. Adaptation is needed to support livelihoods as traditional strategies are no longer sufficient. Gender must also be considered in climate policies and projects to address women's increased burdens. Individual actions like conserving resources and research can contribute to addressing this challenge.

Bimaf iita iarsaf presentation-ibadan 21.05.19

International Institute of Tropical Agriculture

The document discusses managing climate-driven biological risks through a One Health approach. It outlines strategies such as developing early warning and rapid response systems through a farmer interface app connected to pest forecasting tools. The document also discusses building capacity, especially among youth, on modeling species distributions under climate change scenarios. Climate change is expected to impact insect distributions and life cycles, threatening food security. An integrated approach considering human, animal, and ecosystem health is needed to address emerging risks.

In a class of 50

International Institute of Tropical Agriculture

The document discusses the importance of agriculture and food security research given changing weather patterns due to climate change. It notes that 30 students went to a bar, 20 to banks, leaving 10 for farming, but questions who will farm as the work is difficult. It encourages more research to develop crops that can grow in different seasons and conditions to ensure future food security for young people as climate change may impact current agricultural practices. The document concludes by emphasizing the critical role of farmers in providing food and calls for supporting the 22nd symposium of the International Association of Research Scholars in Agricultural and Food Sciences.

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