The ECIS is a turnkey system that provides researchers with an advanced, automated, non-invasive means to monitor cell behavior in real-time and without the use of labels.
A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface.
The core principle behind microarrays is hybridization between two DNA strands, the property of complementary nucleic acid sequences to specifically pair with each other by forming hydrogen bonds between complementary nucleotide base pairs.
Suicide gene therapy is based on the delivery of a gene encoding a cytotoxic protein into tumor cells.
For this, there are two possible strategies:
1. Indirect gene therapy using enzyme-activated pro-drug, which allows the conversion of a pro-drug into a lethal drug into cells.
2. Direct gene therapy using a toxin gene, whose expression can change the stability of the cell membrane and reduce the viability of tumor cells, or correct mutated pro-apoptotic genes, generally tumor suppressor genes that in normal condition induce cell suicide.
Gene Therapy, Somatic cell gene therapy, germ line gene therapy, classical gene therapy, non-classical gene therapy, targets of gene therapy, barriers of gene therapy, ex vivo gene therapy, in vivo gene therapy, vectors for gene delivery, antisense therapy
A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface.
The core principle behind microarrays is hybridization between two DNA strands, the property of complementary nucleic acid sequences to specifically pair with each other by forming hydrogen bonds between complementary nucleotide base pairs.
Suicide gene therapy is based on the delivery of a gene encoding a cytotoxic protein into tumor cells.
For this, there are two possible strategies:
1. Indirect gene therapy using enzyme-activated pro-drug, which allows the conversion of a pro-drug into a lethal drug into cells.
2. Direct gene therapy using a toxin gene, whose expression can change the stability of the cell membrane and reduce the viability of tumor cells, or correct mutated pro-apoptotic genes, generally tumor suppressor genes that in normal condition induce cell suicide.
Gene Therapy, Somatic cell gene therapy, germ line gene therapy, classical gene therapy, non-classical gene therapy, targets of gene therapy, barriers of gene therapy, ex vivo gene therapy, in vivo gene therapy, vectors for gene delivery, antisense therapy
A genome is an organism’s complete set of DNA or complete genetic makeup, The entire DNA complement. It describes the identity and the sequence of genes of an organism.
Genomics is the study of entire genomes(structure, function, evolution, mapping, and editing of genomes)
Executing the sequencing and analysis of entire human genome enables more rapid and effective identification of disease associated genes and provide drug companies with pre validated targets.
Proteomics is the systematic high-throughput separation and characterization of proteins within biological systems./ large scale study of protein and their functions.
Proteomics measures protein expression directly, not via gene expression, thus achieving better accuracy. Current work uses 2-dimensional polyacrylamide gel electrophoresis(2D- PAGE) and mass spectrometry.
New separation and characterization technologies, such as protein microarray and high throughput chromatography are being developed.
mRNA vaccine is a novel vaccine technology, which delivers mRNA that encoding the antigen protein of pathogen to the cell, and expresses the antigen protein, and then stimulates the immune response of the body.
Creative Biolabs has developed non-replicating mRNA vaccine platform, mRNA vaccine platform, mRNA pharmacology optimization platform, and and Self-amplifying mRNA vaccine platform to spport your vaccine researches. If you need more information about mRNA vaccine, please follow us.
Ion channels, types and their importace in managment of diseasesFarazaJaved
This topic covers voltage gated type of ion channel, general structure and functioning of ion channels and involvement of different ion channel types in the pathogenesis as wella as a target for the development of various diseases.
This slideshare conatins detailed overview of immunotheraphy,humanisation of antibodies and its clinical application
this is the topic from cellular and molecular pharmacology of m pharmacy first year
immunotheraphy is further classified to its various types which has been discussed individually
its also conatins various immunotheraphy drugs which has other clinical advantages
2-D electrophoresis is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples.
Two-dimensional electrophoresis was first introduced by O’Farrell and Klose in 1975
2-DGE is a multi-step separation technique in which proteins are solubilized and separated according to charge (pI) in the first dimension using IEF, followed by size (molecular weight, MW) using SDS-PAGE in the second dimension.
The separated proteins are stained with coomassie or silver stain to produce a two-dimensional protein reference map.
A genome is an organism’s complete set of DNA or complete genetic makeup, The entire DNA complement. It describes the identity and the sequence of genes of an organism.
Genomics is the study of entire genomes(structure, function, evolution, mapping, and editing of genomes)
Executing the sequencing and analysis of entire human genome enables more rapid and effective identification of disease associated genes and provide drug companies with pre validated targets.
Proteomics is the systematic high-throughput separation and characterization of proteins within biological systems./ large scale study of protein and their functions.
Proteomics measures protein expression directly, not via gene expression, thus achieving better accuracy. Current work uses 2-dimensional polyacrylamide gel electrophoresis(2D- PAGE) and mass spectrometry.
New separation and characterization technologies, such as protein microarray and high throughput chromatography are being developed.
mRNA vaccine is a novel vaccine technology, which delivers mRNA that encoding the antigen protein of pathogen to the cell, and expresses the antigen protein, and then stimulates the immune response of the body.
Creative Biolabs has developed non-replicating mRNA vaccine platform, mRNA vaccine platform, mRNA pharmacology optimization platform, and and Self-amplifying mRNA vaccine platform to spport your vaccine researches. If you need more information about mRNA vaccine, please follow us.
Ion channels, types and their importace in managment of diseasesFarazaJaved
This topic covers voltage gated type of ion channel, general structure and functioning of ion channels and involvement of different ion channel types in the pathogenesis as wella as a target for the development of various diseases.
This slideshare conatins detailed overview of immunotheraphy,humanisation of antibodies and its clinical application
this is the topic from cellular and molecular pharmacology of m pharmacy first year
immunotheraphy is further classified to its various types which has been discussed individually
its also conatins various immunotheraphy drugs which has other clinical advantages
2-D electrophoresis is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples.
Two-dimensional electrophoresis was first introduced by O’Farrell and Klose in 1975
2-DGE is a multi-step separation technique in which proteins are solubilized and separated according to charge (pI) in the first dimension using IEF, followed by size (molecular weight, MW) using SDS-PAGE in the second dimension.
The separated proteins are stained with coomassie or silver stain to produce a two-dimensional protein reference map.
International Journal of Engineering Research and Development (IJERD)IJERD Editor
journal publishing, how to publish research paper, Call For research paper, international journal, publishing a paper, IJERD, journal of science and technology, how to get a research paper published, publishing a paper, publishing of journal, publishing of research paper, reserach and review articles, IJERD Journal, How to publish your research paper, publish research paper, open access engineering journal, Engineering journal, Mathemetics journal, Physics journal, Chemistry journal, Computer Engineering, Computer Science journal, how to submit your paper, peer reviw journal, indexed journal, reserach and review articles, engineering journal, www.ijerd.com, research journals,
yahoo journals, bing journals, International Journal of Engineering Research and Development, google journals, hard copy of journal,
Investigación en electromicrobiología en donde se utilizan Bacterias especiales para generar electricidad y limpiar agua residual. Electromicrobiology research
Exosomes are smallest extracellular vesicles of size 30 to 100 nm originated from late endosomes. These are released by broad array of cells including B‐ cells, cells, dendritic cells (DCs), T‐cells, epithelial cells,
platelets and many more.
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
SAP Sapphire 2024 - ASUG301 building better apps with SAP Fiori.pdfPeter Spielvogel
Building better applications for business users with SAP Fiori.
• What is SAP Fiori and why it matters to you
• How a better user experience drives measurable business benefits
• How to get started with SAP Fiori today
• How SAP Fiori elements accelerates application development
• How SAP Build Code includes SAP Fiori tools and other generative artificial intelligence capabilities
• How SAP Fiori paves the way for using AI in SAP apps
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
Welocme to ViralQR, your best QR code generator.ViralQR
Welcome to ViralQR, your best QR code generator available on the market!
At ViralQR, we design static and dynamic QR codes. Our mission is to make business operations easier and customer engagement more powerful through the use of QR technology. Be it a small-scale business or a huge enterprise, our easy-to-use platform provides multiple choices that can be tailored according to your company's branding and marketing strategies.
Our Vision
We are here to make the process of creating QR codes easy and smooth, thus enhancing customer interaction and making business more fluid. We very strongly believe in the ability of QR codes to change the world for businesses in their interaction with customers and are set on making that technology accessible and usable far and wide.
Our Achievements
Ever since its inception, we have successfully served many clients by offering QR codes in their marketing, service delivery, and collection of feedback across various industries. Our platform has been recognized for its ease of use and amazing features, which helped a business to make QR codes.
Our Services
At ViralQR, here is a comprehensive suite of services that caters to your very needs:
Static QR Codes: Create free static QR codes. These QR codes are able to store significant information such as URLs, vCards, plain text, emails and SMS, Wi-Fi credentials, and Bitcoin addresses.
Dynamic QR codes: These also have all the advanced features but are subscription-based. They can directly link to PDF files, images, micro-landing pages, social accounts, review forms, business pages, and applications. In addition, they can be branded with CTAs, frames, patterns, colors, and logos to enhance your branding.
Pricing and Packages
Additionally, there is a 14-day free offer to ViralQR, which is an exceptional opportunity for new users to take a feel of this platform. One can easily subscribe from there and experience the full dynamic of using QR codes. The subscription plans are not only meant for business; they are priced very flexibly so that literally every business could afford to benefit from our service.
Why choose us?
ViralQR will provide services for marketing, advertising, catering, retail, and the like. The QR codes can be posted on fliers, packaging, merchandise, and banners, as well as to substitute for cash and cards in a restaurant or coffee shop. With QR codes integrated into your business, improve customer engagement and streamline operations.
Comprehensive Analytics
Subscribers of ViralQR receive detailed analytics and tracking tools in light of having a view of the core values of QR code performance. Our analytics dashboard shows aggregate views and unique views, as well as detailed information about each impression, including time, device, browser, and estimated location by city and country.
So, thank you for choosing ViralQR; we have an offer of nothing but the best in terms of QR code services to meet business diversity!
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
PHP Frameworks: I want to break free (IPC Berlin 2024)Ralf Eggert
In this presentation, we examine the challenges and limitations of relying too heavily on PHP frameworks in web development. We discuss the history of PHP and its frameworks to understand how this dependence has evolved. The focus will be on providing concrete tips and strategies to reduce reliance on these frameworks, based on real-world examples and practical considerations. The goal is to equip developers with the skills and knowledge to create more flexible and future-proof web applications. We'll explore the importance of maintaining autonomy in a rapidly changing tech landscape and how to make informed decisions in PHP development.
This talk is aimed at encouraging a more independent approach to using PHP frameworks, moving towards a more flexible and future-proof approach to PHP development.
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdfPaige Cruz
Monitoring and observability aren’t traditionally found in software curriculums and many of us cobble this knowledge together from whatever vendor or ecosystem we were first introduced to and whatever is a part of your current company’s observability stack.
While the dev and ops silo continues to crumble….many organizations still relegate monitoring & observability as the purview of ops, infra and SRE teams. This is a mistake - achieving a highly observable system requires collaboration up and down the stack.
I, a former op, would like to extend an invitation to all application developers to join the observability party will share these foundational concepts to build on:
3. ECIS measures the change in impedance of a small electrode to AC current flow . The heart of the measurement is a specialized slide that has 8 or 96 individual wells for cell culturing. The base of the device has an array of gold film electrodes that connect to the ECIS electronics to each of the 8 wells.
4. WE CE WE: Working Electrode CE: Counter Electrode The ECIS Electrodes 250 µm
6. Without cells , the current flows unrestrained from the surface of the electrodes.
7. With cells attached and spread upon this region, the current must now flow in the spaces under and between the cells, as the cell membrane are essentially insulators.
8. ECIS Electric Cell-substrate Impedance Sensing A cell morphology biosensor AC Current source ECIS electrode Counter electrode Culture medium (electrolyte) impedance measurement <4 mA/cm 2 4000 Hz The measurement is non-invasive
9. Start of the measurement - no cells attached - resistance is about 2000 ohms. Inoculation , cells anchor and spread on the base of the well including the active 250 µm electrode. With the presence of the cells , their insulating plasma membranes constrain the electrical current and force it to flow in regions beneath and between the cells. This convoluted current path causes large changes in the measured impedance. With the confluent cell layer in place , the resistance now has reached nearly 15,000 ohms. It is important to note that the AC current used in making these measurements (approximately 1 uA) and the resulting voltage drops across the cells (a few mV) has no detectable effects upon them; the measurement is non-invasive.
10. Cell Inoculation (10 5 cells per cm 2 ) ~50 cells on the active electrode NRK cells No cells Data is from a single 250 m dia. Electrode
11. low frequency low frequency AC Frequency determines Current Pathway high frequency
12. AC Frequency in ECIS = Focus Plane in Microscopy Microvilli Stress Fibers Actin Belt MDCK Cells stained with Phalloidin in CLSM
13. Cell-Matrix Contacts 40 kHz Cell-Cell Contacts 400 Hz MDCK Cells In Situ Analysis of Cell Junctions De Novo Formation of Cell-Matrix and Cell-Cell Contacts
14. Analysis of ECIS Raw Data Monitoring the Formation of a Cell Monolayer Cell Spreading 40 kHz MDCK From Joachim Wegener University of Regensburg
15. Analysis of ECIS Raw Data Monitoring the Formation of a Cell Monolayer Cell Spreading 40 kHz MDCK From Joachim Wegener University of Regensburg Barrier Formation 400 Hz
16. Monitoring Endothelial Barrier Function A published model fits the experimental data The measured impedance can be broken down into three parameters 1) Rb, the barrier function of the cell layer 2) Alpha , a term associated with the constricted current flow beneath the cell 3) Cm , the membrane capacitance [Giaever, I. and Keese, C.R., PNAS 81, 3761 (1991)]
17. CHO cells engineered to over-express the muscarinic receptor exposed to the agonist carbachol
18. Data analysis using the ECIS model morphological information Treatment of CHO-M1T cells with carbachol
19.
20. The 16 Well Array Station provides electrical contact for two 8 well ECIS arrays.
21. The 96 Well Array Station provides electrical contact for a single 96 well ECIS Array.
29. Wound Healing Assay ECIS is a fully automated wound healing assay that generates quantitative data in real time-with out opening the incubator door. ECIS measurements are label-free and highly reproducible . Replaces the traditional " scratch " or " scrape " assay. Instead of disrupting the cell layer mechanically with a toothpick, needle or pipette tip and following the migration of cells to "heal" the wound with a microscope, we employ electric signal s to both wound and monitor the healing process. ECIS electrical wounding is only directed at the small population of cells in contact with the active 250um diameter ECIS electrode, producing a well defined 250 um wound. Which can be verified both with the ECIS measurement and with vital staining. Unlike the traditional scrape method, with the ECIS Wound your protein coating is unaffected by the current , it remains fully intact.
33. Cell Attachment and Spreading One of the most direct ECIS measurements is that of the attachment and spreading behaviors of cells. These measurements allow one to study and quantify the interaction of cultured cells with extracellular matrix (ECM) proteins and other macromolecules continuously and in real time.
34. Different cell line behavior The graph shows the attachment and spreading behaviors of two different cell types -namely BSC1 (African Green Monkey Kidney) and NRK (Normal Rat Kidney) cells. Cells were inoculated in ECIS wells at time zero in sufficient number to form a confluent cell layer (100,000 cells per cm). Each cell line studies in this manner will have its own characteristic shape and final values. In these data, the electrodes were not treated in any special manner before the inoculation but are coated with proteins adsorbed from the fetal bovine serum in the culture medium.
35. MDCK II cells inoculated on electrodes pre-coated with various proteins FN fibronectin LAM laminin VN vitronectin BSA bovine serum albumin BSA FN Inoculation Confluent Cell-free Capacitance at high freq. measures the open (cell-free) electrode area Adsorbed proteins alter cell spreading dynamics Monitoring the dynamics of cell attachment & spreading. Capacitance plots are also used to determine and measure cell confluence and proliferation.
36. Used for general morphological changes in cells. Data shows attachment and spreading of cells on various absorbed protein layers.
37. ECIS Measurements of Metastatic Potential We have used the ability of ECIS to detect changes in cell morphology to design whole-cell assays related to the behavior of the cancer cell including metastatic potential.
38. ECIS Measurements of Metastatic Potential The lost of the resistance is due to the lost of integrity of the endothelial cell layer in response to the activities of the cancer cells.
39. Data shows the extravasation of an endothelial layer upon exposure to metastatic cell lines. Challenge with active and heat-killed cells The highly metastatic MLL cell line rapidly breaks down the resistance of the established endothelial cell layer, but the same cells when first heat killed (15 minutes at 56 degrees C) have no effect verifying that the assay is indeed seeing biological activities. In these data all additions are at time zero. challenge of HUVEC cell layers with several different human prostatic carcinoma cell line
40. Angiogenesis Overall, angiogenesis research starts with the monitoring of endothelial cells (barrier function, signaling, cell growth); these observations are then studied to understand their relationship to angiogenesis and tube formation Picture from "Sphingosine 1-phosphate promotes endothelial cell barrier integrity by Edg-dependent cytoskeletal rearrangement" Garcia, 2001.
41. Angiogenesis ECIS is a highly useful in vitro assay to determine endothelial cell growth and its inhibition as well as the barrier function of endothelial monolayers . In preliminary ECIS experiments with VEGF and other growth factors and their inhibitors, results can commonly be correlated with angiogenesis inhibition in vivo. In some cases, the blocking of endothelial cell proliferation has actually been proven to encourage tube formation. Although ECIS cannot directly follow capillary formation in tumor masses, it can monitor the effect of tumor cells upon a normal endothelial cell monolayer in vitro. Monitoring barrier function (permeability), ECIS has successful recorded extravasion of endothelial cell layers by metastatic cells and hence can be used as a tool for testing anti-cancer therapies in vitro.
42. Barrier Function of Cell Layers Measurement of barrier function of cell layers in tissue culture via ECIS has proven to be a significant and rapidly growing use of this technology. Cell layers with very high TER (transcutaneus el. res.) values such as endothelial cells from the brain and epithelial cells exhibiting tight junctions can be measured electrically using porated membrane supports as well as with ECIS.
43. Note that for molecules to travel from the lower chamber to the space above the cell layer, they must also travel in the constricted space beneath the cells to reach the intercellular junctions. Because of this, one does not measure the true barrier function but rather a combination of the constricted paths beneath the cells and the transcellular pathway. The ECIS method is unique in being able to distinguish between these two pathways , since they each affect the measured resistance and capacitance monitored by ECIS in different ways. Using a published mode l , the true barrier function due to intercellular junctions can be extracted from ECIS data. Barrier function Constricted path beneath cells
44. Mathematical modeling can be used to monitor changes in barrier function as shown above following the addition of VEGF to endothelial cells. Barrier Function Data
45. Cell Proliferation Measurements ECIS measurements can be used to monitor cell proliferation, and experiments can be designed to determine how various changes in cell and culture conditions affect the rates at which the cell monolayer approaches confluence. To accomplish these studies, arrays are inoculated with a low cell density providing room for the dividing cell population. As the cell number increases, the amount of electrode area covered with the spread cells grows accordingly, causing the electrode impedance to rise.
46. In Vitro Toxicology The ECIS approach furnishes data that are by nature quantitative , and since the instrument is computer interfaced, very little technician labor is required to acquire large amounts of information . This sort of precision and cost effectiveness are important attributes of ECIS.
47. ECIS Cultureware™ Disposable Electrode Arrays ECIS Cultureware consists of disposable electrode arrays containing gold film electrodes delineated with an insulating film and mounted on a 20 mil optically clear Lexan* polycarbonate substrate . The well top assembly is made of polystyrene. The gold layer is thin enough to allow microscopic observation of the cells using a standard inverted tissue culture microscope. Each well has a gold electrode at the base, which is 250 µm in diameter and has a surface area for cell attachment and growth of 0.9 cm^2. The well holds a maximum volume of about 500 microliters. The ECIS electrode array is placed in an array holder located in the incubator . The final step in the manufacturing process of the arrays involves exposure of the slides to an oxygen plasma that both cleans the electrodes and sterilizes the chambers and lids. (This step also renders both the substrate and the polystyrene walls of the well very hydrophilic or wettable.)
48.
49. Each of the 8 wells contains a single circular 250μm diameter active electrode. Each well has a substrate area of 0.8 cm^2 and a maximum volume of 600μL. On average, with a confluent cell layer, approximately 50 to 100 cells will be measured by the electrode, but even a single cell can be observed. Potential uses include: • Cell migration measurements via automated wound healing. • Studying the effects of agents upon cell motions (micromotion) and cell morphology. • Exceptional signal to noise ratio allows studies of very sparse cultures (including single cells). • The single electrode is ideal for correlated microscopy and ECIS experiments. 8W1E
50. Each of the 8 wells contains ten circular 250 μm diameter active electrodes connected in parallel on a common gold pad. Each well has a substrate area of 0.8 cm^2 and a maximum volume of 600 μL. On average, with a confluent cell layer, approximately 500 to 1000 cells will be measured by the electrodes. Potential uses include: • Recording the activities of more cells over a larger region of the substrate. • Studying the effects of agents upon overall morphology changes. • Reducing fluctuations in impedance due to cell micromotion that may obscure subtle changes in impedance. • Cell migration via automated wound healing is normally possible, providing an average of 10 identical wounds. 8W10E
51. Each of the 8 wells has two sets of 20 circular 250 μm diameter active electrodes located on inter-digitated fingers to provide measurements of cells upon a total of 40 electrodes. Each well has a substrate area of 0.8 cm^2 and a maximum volume of 600 μL. On average, with a confluent layer, approximately 2000 to 4000 cells will be measured by the electrodes. The 10E+ arrays are designed to monitor larger numbers of cells, sampling over the entire bottom of the well. Because of the relatively high number of cells, impedance fluctuations due to micromotion are smoothed out and do not obscure subtle changes in impedance due to the experimental conditions. Potential uses include: • Cell-ECM protein interactions. • Signal transduction assays. • Detection of invasion of endothelial cell layers by metastatic cells. • Barrier function measurements. • Cell proliferation. 8W10E+
52. This array is nearly identical to the 8W1E standard array but the active electrodes come in four different diameters (two wells of each size). Diameters are 250, 100, 50 and 25 μm. Since the number of cells monitored depends upon the active electrode area, these special arrays are used to monitor fewer cells with higher sensitivity. Each well has a substrate area of 0.8 cm^2 and a maximum volume of 600 μL. 8W1EDD
53. Each well in this array has two independent single 250 μm diameter active electrodes.The Medusa array is useful for duplicating readings in the same well or to wound/electroporate one electrode while leaving the other as a control within the same well. When connected to the array holder only the upper four wells are measured. To use the other four wells, the array is turned around and the contact pads at the other end are connected. Each well has a substrate area of 0.8 cm^2 and a volume of 600 μL, and with a confluent cell layer, approximately 50-100 cells will cover each electrode. * As the electrode pattern resembles a many headed snake, the name is taken from Greek mythology and the hair of the infamous Gorgon. 8W2x1E (Medusa*)
54. This array is used to monitor the movement of cells in response to chemical gradients and is the array used in chemotaxis measurements first described by Hadjout, N. et al. (2001) Biotechniques 31 (5) 1130. The measuring electrode in this array is a thin gold line* between two registry marks. Each well has a substrate area of 0.8 cm^2 and a maximum volume of 600 μL. On average, with a confluent layer, approximately 50 to 100 cells will be monitored by the electrode. *The gold line has the same total area as a 250 μm single circular electrode. ChemoTaxis
55. This is a specialized array with 8 active 250 μm diameter electrodes located in the central region at the base of a flow channel measuring 50 mm long, 5 mm in width and 0.4 mm in height. The flow array is useful for ECIS measurements of cells under perfused conditions or to mimic the shear stress endothelial cells experience in vivo. The channel has an area of 2.5 cm^2 and a channel volume of 100 μL. On average, a confluent layer of approximately 50 to 100 cells will be monitored by each electrode. Flow Array
56. 8W1E Printed Circuit Board (PCB) & 8W10E Printed Circuit Board (PCB) This array is similar to the standard 8W1E and 8W10E but uses a printed circuit board as a base layer. All surfaces are compatible with cell culture as in the standard array. This economy array is opaque and optical cell observations are limited to reflection microscopy (upper chambers can be removed following ECIS measurements). PCB