Dehydrogenase
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1) Dehydrogenase enzymes are important indicators of soil microbial activity and play a role in the biological oxidation of soil organic matter by transferring electrons. 2) Dehydrogenase activity is higher under anaerobic conditions and in soils with more red coloring, indicating more microbial activity. It is also influenced by several soil factors like moisture, aeration, organic matter content, pH, temperature, depth and fertilizer/metal content. 3) Dehydrogenase activity can be used to measure disruptions to the soil microbiome from factors like pesticides and trace elements, with higher enzyme activity indicating lower amounts of contaminants.
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1. pdf file on Termmate: https://www.termmate.com/rabia.aziz
2. YouTube: https://www.youtube.com/channel/UCKxWnNdskGHnZFS0h1QRTEA
3. Facebook: https://web.facebook.com/Chemist.Rabia.Aziz/
4. Blogger: https://chemistry-academy.blogspot.com/
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Bioremediation presentation1
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Bioremediation
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This document discusses the importance of soil organic matter. It states that soil organic matter affects chemical and physical soil properties and overall health. It is made up of living and dead biomass and humus. Soil organic matter content typically ranges from 1-6% and provides benefits like improved structure, water retention, and nutrient availability. Maintaining or increasing soil organic matter through practices like reduced tillage, cover crops, and reducing erosion can improve soil quality and sustainability.
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Organic matter is a constituent of soil. It improves soil health. Healthy soil is needed to fill the bowl of hungry millions.
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3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
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Dehydrogenase
- 1. AGM 301 AGRICULTURAL MICROBIOLOGY (1+1) Presented By… Guhan.C, B.Sc.(Hons).Agri, 2018033029, JKKMCAS, Erode, TN.
- 2. INTRODUCTION • A dehydrogenase (also called DHA or DHase) is an enzyme belonging to the group of oxidoreductase that oxidizes a substrate by reducing an electron acceptor. • Determination of DHA in the soil samples gives information about biological characteristic of the soil. • Soil DHA strongly increases under anaerobic conditions. • Red colors of soil - DHA is higher. • Light red colors soil - lower DHA.
- 3. DEHYDROGENASE ACTIVITY IN SOIL • Dehydrogenases are the important indicator of soil microbial activity - microbial oxido-reduction processes. • DHA play a significant role in the biological oxidation of SOM by transferring hydrogen from organic substrates to inorganic acceptors. • Many specific dehydrogenases transfer hydrogen to either nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate. • DHA serves as an indicator of the microbiological redox- systems. • DHA is considered a good and adequate measure of microbial oxidative activities in soil.
- 4. Contd… • DHA is known to oxidize SOM by transfering protons and electrons from substrate to acceptors. • DHA is used to measure any disruption caused by pesticides, trace elements to the soil. • Higher enzyme activity - lower the amount of pesticides.
- 5. SOIL FACTORS INFLUENCE DHA… 1. Soil Moisture. • Water availability - affects soil enzymatic activities. • Soils dry - water potential ↑ - enzyme activity ↓. • Wet soils - increased soil solution soluble OM - bacterial population ↑. 2. Soil aeration. • Soil water content ↓ - Oxygen Diffusion Rate (ODR) and redox potential ↑ - favourable for DHA.
- 6. 3. Organic Matter Content. • Soil OM as an indicator of soil quality. • Its character of nutrient sink and source enhance soil physical and chemical properties, and also promote biological activity. • The higher OM - higher microbial biomass - higher enzyme production. 4. pH. Enzyme activities tend to increase with soil pH.
- 7. 5. Temperature. • Its activity is highest at a temperature close to optimum temp (5 – 30 ̊ C) for mo growth and their development. • The rate of enzyme catalysis increases with increase in temperature until the unfavorable temperature, at which enzyme becomes denaturized and hence its activity reduces. 6. Depth of soil Profile. • Highest mo abundance in the surface layer of the soil profile (till to the depth of 30 cm), • At deepest part of the soil the no.of.microbial cells is limited, and also DHA level at diminishing trend.
- 8. 7. Fertilizer. • Inorganic fertilizers had relatively less effect on soil enzymes activity than organic fertilizers. • DHA usually reached higher level in the organic treatments. 8. Heavy metals. • Heavy metals can reduce enzyme activity by interacting with the enzyme-substrate complex, denaturing the enzyme protein or interacting with the protein-active groups.