Plant defense strategies which act against the plant pathogen attack are explained in short. The major plant defense strategies are 1) Oxidative burst 2) Phenolics 3) Hypersensitive response (HR) 4) Phytoalexins.
Molecular basis of plant resistance and defense responses to pathogensSenthil Natesan
In response to pathogen attack, plants have evolved sophisticated defense mechanisms to delay or arrest pathogen growth.Unlike animals, plants lack a circulating immune system recognizing microbial pathogens. Plant cells are more autonomous in their defense mechanisms and rely on the innate immune capacity of each cell and systemic signals that disseminate from infection sites (Jones and Dangl, 2006). Plant innate immunity consists of preformed physical and chemical barriers (such as leaf hairs, rigid cell walls, pre-existing antimicrobial compounds) and induced defenses. Should an invading microbe successfully breach the pre-formed barriers, it may be recognized by the plant, resulting in the activation of cellular defense responses that stop or restrict further development of the invader.
Molecular basis of plant resistance and defense responses to pathogensSenthil Natesan
In response to pathogen attack, plants have evolved sophisticated defense mechanisms to delay or arrest pathogen growth.Unlike animals, plants lack a circulating immune system recognizing microbial pathogens. Plant cells are more autonomous in their defense mechanisms and rely on the innate immune capacity of each cell and systemic signals that disseminate from infection sites (Jones and Dangl, 2006). Plant innate immunity consists of preformed physical and chemical barriers (such as leaf hairs, rigid cell walls, pre-existing antimicrobial compounds) and induced defenses. Should an invading microbe successfully breach the pre-formed barriers, it may be recognized by the plant, resulting in the activation of cellular defense responses that stop or restrict further development of the invader.
Effect of environment and nutrition on plant disease developmentparnavi kadam
BRIEF AND PRECISE POINTS ON PLANT DISEASE DEVELOPMENT. IT MOSTLY FOCUSES ON HOW THE FACTORS AFFECT THE MICROBES AND THEN THEIR MICROBIAL EFFECT ON DISEASE DEVELOPMENT.
Plants have array of defense response against biotic stresses which could be either structural reinforcement, release of chemicals, and defense gene expression against invading organisms. The physical barriers are trichoms, waxy cuticle, thick cell wall. Once the pathogen overcomes the first line of defense, basal or innate defense response comes into play. Pathogens secrete some conserved molecules known as Pathogen Associated Molecular Pattern (PAMP/MAMP), which are recognized by transmembrane receptors present in the plasma membrane and initiate a series of signal cascade reaction which ultimately leads to activation of various defense related genes. Apart from inducing the expression of defense related genes, it also triggers a hypersensitive reaction (HR) which cause deliberate cell death at the site of infection and limit the pathogen access to water and nutrient by sacrificing a few cells in order to save the rest of the plant. Once HR is triggered, plant tissue may become highly resistant to a broad range of pathogens for an extended period of time. This phenomenon is called Systemic Acquired Resistance (SAR).
Plants respond to herbivory is a similar manner as described above. The biochemical mechanisms of defense against the herbivores are wide-ranging, highly dynamic, and are mediated both by direct and indirect defenses. The defensive compounds are either produced constitutively or in response to plant damage, and affect feeding, growth, and survival of herbivores. In addition, plants also release volatile organic compounds that attract the natural enemies of the herbivores. These strategies either act independently or in conjunction with each other. However, our understanding of these defensive mechanisms is still limited. Induced resistance could be exploited as an important tool for the pest management to minimize the amounts of insecticides used for pest control. Host plant resistance to insects, particularly, induced resistance, can also be manipulated with the use of chemical elicitors of secondary metabolites, which confer resistance to insects. By understanding the mechanisms of induced resistance, we can predict the herbivores that are likely to be affected by inducing responses. The elicitors of induced responses can be sprayed on crop plants to build up the natural defense system against damage caused by herbivores. The induced responses can also be genetically engineered, so that the defensive compounds are constitutively produced in plants challenged by the herbivory. Induced resistance can be exploited for developing crop cultivars, which readily produce the inducible response upon mild infestation, and can act as one of components of integrated pest management for sustainable crop production.
Systemic Acquired Resistance (SAR) and it’s Significance in Plant Disease Ma...Ankit Chaudhari
Systemic Acquired Resistance (SAR) is a mechanism of induced defense that confers long-lasting protection against a broad spectrum of microorganisms and pests. Presently disease control is largely based on the use of hazardous chemicals viz., fungicides, bactericides and insecticides for either direct or indirect disease management. The hazardous natures of the products on the environment, human and animal health strongly necessitates the search for new safer means of disease control. SAR have high potential to diminish the use of toxic chemicals in the agriculture and has emerged as an alternative, non-conventional, non-biocidal and eco-friendly approach for plant protection and hence for sustainable agriculture. SAR requires the signal molecule salicylic acid (SA) and is associated with accumulation of pathogenesis-related proteins, which are thought to contribute to resistance.
plant pathogen interaction
different types of pathogens
gene for gene hypothesis
direct receptor model
Elicitor receptor model
suppersor repressor model
gaurd hypothesis
SURVIVAL AND DISPERSAL OF PHYTOPATHOGENIC BACTERIA.pdfOm Prakash
SURVIVAL OF PHYTOPATHOGENIC BACTERIA
Phytopathogenic bacteria have the ability to survive both for longer & shorter periods including soil, seed, diseased crop debris, weed host, and insect vectors.
DISPERSAL OF PLANT PATHOGENIC BACTERIA
To make a healthy plant diseased, the first requirement of a pathogen is to spread its inoculum (primary as well as secondary) from the source of survival to the susceptible parts of a healthy plant. The spread of a plant pathogen within the general area in which it is already established is called “dispersal” or “dissemination”.
Moving the inoculum only a few inches and transporting it for hundreds of miles both constitute its dispersal or dissemination. However, pathogen dispersal is not necessary only for the spread of diseases but also for the continuity of the life-cycle and evolution of the pathogen. Detailed knowledge of pathogen-dispersal is essential to find out effective control measures for diseases because the possibilities of preventing dispersal and thereby breaking the infection chain always exist.
Effect of environment and nutrition on plant disease developmentparnavi kadam
BRIEF AND PRECISE POINTS ON PLANT DISEASE DEVELOPMENT. IT MOSTLY FOCUSES ON HOW THE FACTORS AFFECT THE MICROBES AND THEN THEIR MICROBIAL EFFECT ON DISEASE DEVELOPMENT.
Plants have array of defense response against biotic stresses which could be either structural reinforcement, release of chemicals, and defense gene expression against invading organisms. The physical barriers are trichoms, waxy cuticle, thick cell wall. Once the pathogen overcomes the first line of defense, basal or innate defense response comes into play. Pathogens secrete some conserved molecules known as Pathogen Associated Molecular Pattern (PAMP/MAMP), which are recognized by transmembrane receptors present in the plasma membrane and initiate a series of signal cascade reaction which ultimately leads to activation of various defense related genes. Apart from inducing the expression of defense related genes, it also triggers a hypersensitive reaction (HR) which cause deliberate cell death at the site of infection and limit the pathogen access to water and nutrient by sacrificing a few cells in order to save the rest of the plant. Once HR is triggered, plant tissue may become highly resistant to a broad range of pathogens for an extended period of time. This phenomenon is called Systemic Acquired Resistance (SAR).
Plants respond to herbivory is a similar manner as described above. The biochemical mechanisms of defense against the herbivores are wide-ranging, highly dynamic, and are mediated both by direct and indirect defenses. The defensive compounds are either produced constitutively or in response to plant damage, and affect feeding, growth, and survival of herbivores. In addition, plants also release volatile organic compounds that attract the natural enemies of the herbivores. These strategies either act independently or in conjunction with each other. However, our understanding of these defensive mechanisms is still limited. Induced resistance could be exploited as an important tool for the pest management to minimize the amounts of insecticides used for pest control. Host plant resistance to insects, particularly, induced resistance, can also be manipulated with the use of chemical elicitors of secondary metabolites, which confer resistance to insects. By understanding the mechanisms of induced resistance, we can predict the herbivores that are likely to be affected by inducing responses. The elicitors of induced responses can be sprayed on crop plants to build up the natural defense system against damage caused by herbivores. The induced responses can also be genetically engineered, so that the defensive compounds are constitutively produced in plants challenged by the herbivory. Induced resistance can be exploited for developing crop cultivars, which readily produce the inducible response upon mild infestation, and can act as one of components of integrated pest management for sustainable crop production.
Systemic Acquired Resistance (SAR) and it’s Significance in Plant Disease Ma...Ankit Chaudhari
Systemic Acquired Resistance (SAR) is a mechanism of induced defense that confers long-lasting protection against a broad spectrum of microorganisms and pests. Presently disease control is largely based on the use of hazardous chemicals viz., fungicides, bactericides and insecticides for either direct or indirect disease management. The hazardous natures of the products on the environment, human and animal health strongly necessitates the search for new safer means of disease control. SAR have high potential to diminish the use of toxic chemicals in the agriculture and has emerged as an alternative, non-conventional, non-biocidal and eco-friendly approach for plant protection and hence for sustainable agriculture. SAR requires the signal molecule salicylic acid (SA) and is associated with accumulation of pathogenesis-related proteins, which are thought to contribute to resistance.
plant pathogen interaction
different types of pathogens
gene for gene hypothesis
direct receptor model
Elicitor receptor model
suppersor repressor model
gaurd hypothesis
SURVIVAL AND DISPERSAL OF PHYTOPATHOGENIC BACTERIA.pdfOm Prakash
SURVIVAL OF PHYTOPATHOGENIC BACTERIA
Phytopathogenic bacteria have the ability to survive both for longer & shorter periods including soil, seed, diseased crop debris, weed host, and insect vectors.
DISPERSAL OF PLANT PATHOGENIC BACTERIA
To make a healthy plant diseased, the first requirement of a pathogen is to spread its inoculum (primary as well as secondary) from the source of survival to the susceptible parts of a healthy plant. The spread of a plant pathogen within the general area in which it is already established is called “dispersal” or “dissemination”.
Moving the inoculum only a few inches and transporting it for hundreds of miles both constitute its dispersal or dissemination. However, pathogen dispersal is not necessary only for the spread of diseases but also for the continuity of the life-cycle and evolution of the pathogen. Detailed knowledge of pathogen-dispersal is essential to find out effective control measures for diseases because the possibilities of preventing dispersal and thereby breaking the infection chain always exist.
the balance between free radicals and antioxidants is a necessity for proper physiological work in the body, but when the difference between them produces a so-called oxidative stress
Importance and use of micro-nutrient 'Boron' in vegetable cropsHarshvardhan Gaikwad
The use and significance of essential micro-nutrient 'Boron' in different vegetable crops is explained in this powerpoint presentation. The role, function, general boron deficiency and toxicity symptoms different vegetable crops, boron requirement and critical level are mentioned and illustrated here.
Doctoral seminar: Management of viral diseases in pulses and oilseedsHarshvardhan Gaikwad
PL.PATH-691 (Doctoral seminar), I presented on topic: Management of viral diseases in pulses and oilseeds. In which, I explained virus, history of virus, classification of plant virus, different viral diseases of pulses and oilseed crops, their management and three case studies. As we know that, virus always alters its genetic material and it is difficult and tedious to manage plant viral diseases.
Pl. PATH-605 Introduction to certification. International scenario of certifi...Harshvardhan Gaikwad
Pl. PATH-605 (Principles and Procedure of Certification). During this course of Ph,D., I presented on topic: Introduction to certification. International scenario of certification and role of ISTA, EPPO, OECD etc. in certification and quality control. In which the seed certification and certification authorities are explained.
During III semester of Ph.D. program, I presented on a topic- Signal Transduction – Salicylic Acid Pathway. The Salicylic acid plays the role in induction of flowering, in disease resistance (HR, SAR activation). In this presentation, I have tried to explain complex pathway of salicylic acid production during the signal tranduction.
This presentation is about Nematode management options for organic and precision farming. In this presentation care and management practices used for nematode control are explained, some of them are 1) Resistant crop variety 2) Crop rotation 3) Soil solarization 4) Biological control etc.
PL. PATH - 601 (Advanced Mycology) Topic- Advances in nomenclature and taxono...Harshvardhan Gaikwad
This is my presentation for Advanced Mycology in 1st semester of Ph.D. program. There are advances in nomenclature and taxonomic criteria and their procedures. The history of nomenclature and some changes done in 18th international botanical congress are explained here.
'Genomics' is nothing but the study of entire genetic compliment of an organism. Plant genomics is study of plant genome. This is my topic of M.Sc. course 'Plant biotechnology'.
Outline of Research Work (M.Sc.). Research title: "Chitosan as a natural pote...Harshvardhan Gaikwad
This power point presentation is the outline of research work of my M.Sc. research program. The research work was conducted at College of Agriculture, Pune, National Agriculture Research Project, Ganeshkhind, Pune and Vasantdada Sugar Institute, Manjari, Pune.
M.Sc. (Master's) Seminar on topic "Role of chemicals in plant disease managem...Harshvardhan Gaikwad
The importance and role of chemicals/ fungicides in plant disease management is the major objective of plant pathology. The need based, effective, ecofriendly application of chemical fungicides can leads sustainable agriculture and food production.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
2. Defense Strategies
Oxidative Burst :
It involves generation of Reactive Oxygen Species (ROS), primarily
singlet oxygen 1O2, superoxide O2
-, H2O2 and OH free radicals, which
trigger gene expression, cross-linking of host cell wall and initiation of
later defense responses. The ROS can kill the micro organisms directly
at the site of infection, but they can kill host cells also. The ROS can
directly trigger the hypersensitive response and, therefore, regulation
of their concentration is important.
3. Phenolics
Resistant Varieties- high phenols
Shikimic acid Pathway, Acetic acid pathway
Common phenols- Pre existing, Conc. Not sufficient, synthesis and
accumulation
Phytoalexins
Chlorogenic acid present in potato when it is affected with
Streptomyces scabies, (scab) and Verticillium wilt.
The peel of mango contains a mixture of antifungal resorcinols.
Tannic acid protect tobacco plant from infn by tobacco mosaic virus.
4. Hypersensitive Response (HR)
The final stage of rapid defence response. It is characterized by rapid
localized cell and tissue death around the site of infection.
HR is expressed only as a result of specific recognition between plant
and pathogen. The pathogen remained confined to necrotic lesions.
The cells surrounding necrotic region are highly reacting and initiate
synthesis of antimicrobial compounds (phytoalexins) and signal
molecules i.e. salicylic acid that induces non specific, SAR against
subsequent infections.
5. Phytoalexins
Low molecular wt. antimicrobial compounds produced by plants in
response to infection.
Associated with HR
HR in minuets, phytoalexins accumulation takes hours.
Not translocated and non- persistant (indefinitely continuous).
Over 350 phytoalexins are known in over 100 plant species.
Ipomeamarone in Ipomea batata.
Orchinol in Orachis militaris. orchid