Dave Erickson - Plants
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The document discusses processes for DNA barcoding in plants, including DNA extraction from plant tissue, PCR amplification of target genes, and sequencing and analysis. It provides details on using 96-well plates or single tubes for tissue disruption and lysis. It recommends checking and adjusting the pH of extracted DNA. For PCR, it suggests diluting DNA and using a high number of cycles for some gene regions. ExoSap purification and typical sequencing reaction conditions are also outlined.
![Making Barcoding work in Plants David Erickson Department of Botany Smithsonian Institution [email_address]](https://image.slidesharecdn.com/tue20-20napier2010220-20160020-20dave20erikson20-cbol20plant20methods1-111206195503-phpapp02/85/Dave-Erickson-Plants-1-320.jpg)
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Dave Erickson - Plants
- 1. Making Barcoding work in Plants David Erickson Department of Botany Smithsonian Institution [email_address]