Flyer of our new product Cleanpid® Easy Purification Kit for detecting Legionella. Cleanpid is for purification the sample before doing the PCR. After using CLEANPID you can collect the Legionella cells and with freeze/thaw cycles you can obtain the DNA and with PCR, the customer could say if there is or not Legionella presence. Legionellae DNA extraction is particularly problematic when different types of microorganisms may be present. Cleanpid® Easy Purification kit is the simple method for the separation and purification of Legionella in water samples. The method is based on Legionella binding magnetic beads for capturing cells (immunomagnetic separation). No columns or centrifugations are necessary. A summary of the advantages of Cleanpid is:  Quick and Easy-To-Use  The kit only requires a magnetic rack (we provide it)  Improves the sensitivity and reproducibility of qPCR  Reduction of false positive rate for PCR: o Detects only legionella viable cells (not exogenous DNA, not other microbiota...)  Reduction of false negative rate for PCR: o Obtains a larger amount of DNA from the water samples (avoid dilution of the DNA samples for eliminating inhibitors)  The purified organism is additionally suitable for DNA extraction by simply freeze/thaw cycles (simply and cost-efficient lysis) http://www.biotica.es/en/Products/Cleanpid%20Easy%20Purification%20kit%20for%20Legionella

1. Reduce the cost of….
Unconclusive results
Inhibition of detection
Complex matrices
Microbiota
Cleanpid® Provides you the purified
sample that you need for better
Legionella detection
Legionellae DNA extraction is particularly problematic
when different types of microorganisms may be
present. Clean your sample and then work with
Legionella alone.
Cleanpid® Easy Purification kit is the
simple method for the separation and
purification of Legionella in water
samples
Biótica Bioquímica Analítica S.L.
ScientificandTechnologicalParkof
UniversityJaumeI
CampusRiuSec-BuildingEspaitec2
Groundfloor,laboratory2
12071-CastellóndelaPlana(Spain)
Phone:+34964108131Fax:+34964737790
info@biotica.es
www.biotica.es

2. bCleanpid®improvesthesensitivityand
reproducibilityofqPCR.
Ct. number of
amplification cycles
Number of test
Kit content
Funcionality
Processing time
Hands - on time
Highly robust
performance
Specific
instrumentation
Storage
Reagents for 50 test (Ref. 611-10-00)
Capturing reagent, Working buffer
The purified organism is suitable for DNA extraction
by simply freeze/thaw cycles (symply and
cost-efficient lysis)
60minutes foranynumberof samples,approximately
Quick and Easy-To-Use
Produces consistent and reproducible results
The kit only requires a magnetic rack
2 - 8ºC
technicalfeatures
Schrader C, Schielke A, Ellerbroek L and Johne R: PCR inhibitors –
occurrence,propertiesandremoval.2012,JournalofAppliedMicrobiolo-
gy, 113, 1014—1026.doi:10.1111/j.1365-2672.2012.05384.x
BEFORE AFTER
“Immunocapture methods are very efficient in
removal of PCR inhibitors because the pathogen can
be specifically separated from the sample matrix and
the accordant inhibitors
”
Add Legionella binding magnetic
beads to the sample contained in
the capture tube and mix by
repeated pipetting. Incubate for
60 min with gently shaking.
Place the tube on the magnetic
racktoseparatemagneticbeads
and discard supernatant.
Resuspend the bead pellet in the
working buffer by repeated gently
pipetting. Retain again and discard
supernatant(3X).
Legionellabindingmagneticbeadsfor
capturingcells
(immunomagneticseparation)
method
tube complexes
magnetic
beads-
Legionella
magnet
Resuspended magnetic beads
Freeze/Thaw cycles
Magnetic retention
Magnetic beads Supernatant(DNA)
Ready to PCR assay!
The PCR is prone to inhibitory substances which may be
present in the environmental sample and which may
affect the sensitivity of the assay or even lead to false
negative results.
Moreover, to detect Legionella, the sample is
usually concentrated to very small volumes
and this often results in concurrent
concentration of the different inhibitors
and increased interference with PCR.
The dilution of the sample or the
extracted DNA result in a dilution of
the inhibitors but also result in a
decrease of the sensitivity.
The discarding of PCR inhibitors in one
step by Cleanpid allows obtaining a larger
amount of DNA from the water samples
and avoiding dilution, therefore helping to
reduce false negative rate.
Reduction of false positive rate for
PCR
Reduction of false negative rate for
PCR
The false positive is due to PCR quantifies total DNA of
Legionella in a sample, which proceed not only from living cells
but also from damaged or dead cells, and free DNA (exogenous).
Thefalsepositivecouldalsobeduetocross-reactivityinthePCR
assay with other accompanying microorganisms in the sample
The selective separation of intact cells by Cleanpid allows
detecting only the DNA of potentially viable cells of Legionella,
providing a reduction of false positive rate.
enefits
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