Are ranaviruses capable of causing local population extinctions and contributing to species declines? By Dr. Matt Gray (University of Tennessee, Center for Wildlife Health)
ABSTRACT
THE 4R GENOME DUPLICATION IN SALMONINE FISHES:
INSIGHTS FROM CONSERVED NON-CODING ELEMENTS
Anibal H. Castillo Co-Advisors:
University of Guelph, 2008 Professors Dr. Moira M. Ferguson
Dr. Roy G. Danzmann
Gene and genome duplications are important processes in evolution. Salmonids are ideal animal model systems in which to study these processes, as they originated from a tetraploid ancestor. Conserved non-coding elements (CNEs) are of interest because of their highly conserved DNA consensus motifs spanning lineages as diverse and divergent as humans and fish. The main goal of this study is to test CNEs as a tool to study genome duplications and to revisit the “4R” hypothesis and phylogeny of Salmonine fishes (Salmonidae) Salmo salar, Salvelinus alpinus and Oncorhynchus mykiss through the study of copy number and nucleotide variation in six pairs of CNEs. Allele numbers for most CNE sequence pairs are consistent with the 4R hypothesis, as is the symmetric phylogenetic topology shown by some CNE pairs; the estimated date of CNE duplication is consistent with the only reported range of 25-100Mya. However, the phylogenetic relationships within Salmoninae remain unresolved.
Ecology of Ranaviruses: State of Understandingmgray11
Presentation on the basic ecology of ranaviruses and mechanisms that may be contribute to this pathogen's emergence. By Dr. Matt Gray (University of Tennessee, Center for Wildlife Health)
Are ranaviruses capable of causing local population extinctions and contributing to species declines? By Dr. Matt Gray (University of Tennessee, Center for Wildlife Health)
ABSTRACT
THE 4R GENOME DUPLICATION IN SALMONINE FISHES:
INSIGHTS FROM CONSERVED NON-CODING ELEMENTS
Anibal H. Castillo Co-Advisors:
University of Guelph, 2008 Professors Dr. Moira M. Ferguson
Dr. Roy G. Danzmann
Gene and genome duplications are important processes in evolution. Salmonids are ideal animal model systems in which to study these processes, as they originated from a tetraploid ancestor. Conserved non-coding elements (CNEs) are of interest because of their highly conserved DNA consensus motifs spanning lineages as diverse and divergent as humans and fish. The main goal of this study is to test CNEs as a tool to study genome duplications and to revisit the “4R” hypothesis and phylogeny of Salmonine fishes (Salmonidae) Salmo salar, Salvelinus alpinus and Oncorhynchus mykiss through the study of copy number and nucleotide variation in six pairs of CNEs. Allele numbers for most CNE sequence pairs are consistent with the 4R hypothesis, as is the symmetric phylogenetic topology shown by some CNE pairs; the estimated date of CNE duplication is consistent with the only reported range of 25-100Mya. However, the phylogenetic relationships within Salmoninae remain unresolved.
Ecology of Ranaviruses: State of Understandingmgray11
Presentation on the basic ecology of ranaviruses and mechanisms that may be contribute to this pathogen's emergence. By Dr. Matt Gray (University of Tennessee, Center for Wildlife Health)
Presentation 2.5 Ecology, virulence factors and global spread of pathogenic V...ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
FAO Second International Technical Seminar/Workshop on Acute hepatopancreatic necrosis disease (AHPND) There is a way forward! FAO Technical Cooperation Programme: TCP/INT/3501 and TCP/INT/3502.
DOI: 10.21276/ijlssr.2016.2.3.19
ABSTRACT- By using gamma rays (physical) & EMS (chemical) mutagens the various genetic variability parameters
were estimated of two soybean cultivars i.e. PKV-1 & JS-335. Characters studied i.e. Plant height, no. of branches per
plant, no. of clusters per plant, no. of pods per plant, yield per plant, 100 grain wt. shows that genotypic coefficient of
variation (G.C.V.) & phenotypic coefficient of variation (PCV), heritability was significantly high. In both the varieties, all
the mutagenic treatments were effective in inducing genetic variability.
Key-words- Gamma rays, EMS, Mutagens, Genetic Variability
The study of bugs relating to crime scene investigation. How the professionals work together with insects to determine time of death and any other important clues needed to solve a murder investigation. I took this class my freshman year of college.
DNA Barcoding: A simple way of identifying species by DNAmarkstoeckle
DNA barcoding makes it easier for experts and non- experts to identify species including from bits and pieces, immature forms, and those with many close look-alikes. Applications include health, environment, and education. High school students are using DNA barcoding to explore the world around them and make scientific discoveries. Like a giant Wikipedia entry, the multitude of researchers depositing DNA barcodes in GenBank are creating the first large-scale maps of the genetic structure of biodiversity.
This presentation discusses various aspects of wildlife crime management and conservation, some aspects related to genetics and new discoveries and techniques are also added.
Rainbow Trout: Introduction and Importancemeemail377
Rainbow trout, Oncorhynchus mykiss, are colorful freshwater fish native to North America, renowned for their striking iridescent hues. Sporting a streamlined body and distinctive pink stripe along their sides, rainbow trout inhabit cold, clear streams and lakes. They are prized by anglers for their spirited fights and delicious flesh. Rainbow trout are opportunistic feeders, consuming insects, crustaceans, and small fish. They play crucial roles in freshwater ecosystems as both predators and prey, contributing to the balance of aquatic food webs.
Presentation 2.5 Ecology, virulence factors and global spread of pathogenic V...ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
FAO Second International Technical Seminar/Workshop on Acute hepatopancreatic necrosis disease (AHPND) There is a way forward! FAO Technical Cooperation Programme: TCP/INT/3501 and TCP/INT/3502.
DOI: 10.21276/ijlssr.2016.2.3.19
ABSTRACT- By using gamma rays (physical) & EMS (chemical) mutagens the various genetic variability parameters
were estimated of two soybean cultivars i.e. PKV-1 & JS-335. Characters studied i.e. Plant height, no. of branches per
plant, no. of clusters per plant, no. of pods per plant, yield per plant, 100 grain wt. shows that genotypic coefficient of
variation (G.C.V.) & phenotypic coefficient of variation (PCV), heritability was significantly high. In both the varieties, all
the mutagenic treatments were effective in inducing genetic variability.
Key-words- Gamma rays, EMS, Mutagens, Genetic Variability
The study of bugs relating to crime scene investigation. How the professionals work together with insects to determine time of death and any other important clues needed to solve a murder investigation. I took this class my freshman year of college.
DNA Barcoding: A simple way of identifying species by DNAmarkstoeckle
DNA barcoding makes it easier for experts and non- experts to identify species including from bits and pieces, immature forms, and those with many close look-alikes. Applications include health, environment, and education. High school students are using DNA barcoding to explore the world around them and make scientific discoveries. Like a giant Wikipedia entry, the multitude of researchers depositing DNA barcodes in GenBank are creating the first large-scale maps of the genetic structure of biodiversity.
This presentation discusses various aspects of wildlife crime management and conservation, some aspects related to genetics and new discoveries and techniques are also added.
Rainbow Trout: Introduction and Importancemeemail377
Rainbow trout, Oncorhynchus mykiss, are colorful freshwater fish native to North America, renowned for their striking iridescent hues. Sporting a streamlined body and distinctive pink stripe along their sides, rainbow trout inhabit cold, clear streams and lakes. They are prized by anglers for their spirited fights and delicious flesh. Rainbow trout are opportunistic feeders, consuming insects, crustaceans, and small fish. They play crucial roles in freshwater ecosystems as both predators and prey, contributing to the balance of aquatic food webs.
Saving our not so Furry Friends - Long live the freshwater crocodile by Anton...Art4Agriculture
This is the story of how Antonia found herself in the middle of nowhere wrestling crocs on a study with PhD student Ruchira Somaweera.
An experience that left her with more than an award winning thong tan on arrival back home to a little town called Sydney
Population Structure & Genetic Improvement in LivestockGolden Helix Inc
The genetic improvement of livestock has been a hot topic for almost a century, bringing together researchers, industry, and producers to work towards a common goal. Many countries currently employ extensive genetic selection programs in their cattle with pigs, sheep, and chicken close behind.
In this webcast, Heather J. Huson, Ph.D. from Cornell University will focus on population dynamics and trait association in cattle and goats using high density SNP datasets. Population structure plays a critical role in understanding the relatedness among livestock, ancestral origins of traits, and identification of unique sub-populations or breeds for production improvement and conservation. This also lays the foundation for understanding and improving species such as the goat which is a vital food source in developing countries but has little recorded production or health data.
Understanding population structure is essential for designing complex trait association studies such as those related to production and health characteristics. Here, Huson shows examples of her lab's investigation into population structure in both goats and cattle to identify distinct groups and study traits such as thermo-tolerance.
Dr. Christie Mayo - Vesicular Stomatitis Virus UpdateJohn Blue
Vesicular Stomatitis Virus Update - Dr. Christie Mayo, DVM, Colorado State University, from the 2015 NIAA Annual Conference titled 'Water and the Future of Animal Agriculture', March 23 - March 26, 2015, Indianapolis, IN, USA.
More presentations at http://www.trufflemedia.com/agmedia/conference/2015_niaa_water_future_animal_ag
Character-based DNA Barcoding allows for integration of geography, ecology and morphology: The discovery of a cryptic species complex in dragonflies using Caos.
More from Consortium for the Barcode of Life (CBOL) (20)
Essentials of Automations: The Art of Triggers and Actions in FMESafe Software
In this second installment of our Essentials of Automations webinar series, we’ll explore the landscape of triggers and actions, guiding you through the nuances of authoring and adapting workspaces for seamless automations. Gain an understanding of the full spectrum of triggers and actions available in FME, empowering you to enhance your workspaces for efficient automation.
We’ll kick things off by showcasing the most commonly used event-based triggers, introducing you to various automation workflows like manual triggers, schedules, directory watchers, and more. Plus, see how these elements play out in real scenarios.
Whether you’re tweaking your current setup or building from the ground up, this session will arm you with the tools and insights needed to transform your FME usage into a powerhouse of productivity. Join us to discover effective strategies that simplify complex processes, enhancing your productivity and transforming your data management practices with FME. Let’s turn complexity into clarity and make your workspaces work wonders!
Pushing the limits of ePRTC: 100ns holdover for 100 daysAdtran
At WSTS 2024, Alon Stern explored the topic of parametric holdover and explained how recent research findings can be implemented in real-world PNT networks to achieve 100 nanoseconds of accuracy for up to 100 days.
The Art of the Pitch: WordPress Relationships and SalesLaura Byrne
Clients don’t know what they don’t know. What web solutions are right for them? How does WordPress come into the picture? How do you make sure you understand scope and timeline? What do you do if sometime changes?
All these questions and more will be explored as we talk about matching clients’ needs with what your agency offers without pulling teeth or pulling your hair out. Practical tips, and strategies for successful relationship building that leads to closing the deal.
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
The Metaverse and AI: how can decision-makers harness the Metaverse for their...Jen Stirrup
The Metaverse is popularized in science fiction, and now it is becoming closer to being a part of our daily lives through the use of social media and shopping companies. How can businesses survive in a world where Artificial Intelligence is becoming the present as well as the future of technology, and how does the Metaverse fit into business strategy when futurist ideas are developing into reality at accelerated rates? How do we do this when our data isn't up to scratch? How can we move towards success with our data so we are set up for the Metaverse when it arrives?
How can you help your company evolve, adapt, and succeed using Artificial Intelligence and the Metaverse to stay ahead of the competition? What are the potential issues, complications, and benefits that these technologies could bring to us and our organizations? In this session, Jen Stirrup will explain how to start thinking about these technologies as an organisation.
Alt. GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using ...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdfPaige Cruz
Monitoring and observability aren’t traditionally found in software curriculums and many of us cobble this knowledge together from whatever vendor or ecosystem we were first introduced to and whatever is a part of your current company’s observability stack.
While the dev and ops silo continues to crumble….many organizations still relegate monitoring & observability as the purview of ops, infra and SRE teams. This is a mistake - achieving a highly observable system requires collaboration up and down the stack.
I, a former op, would like to extend an invitation to all application developers to join the observability party will share these foundational concepts to build on:
Climate Impact of Software Testing at Nordic Testing DaysKari Kakkonen
My slides at Nordic Testing Days 6.6.2024
Climate impact / sustainability of software testing discussed on the talk. ICT and testing must carry their part of global responsibility to help with the climat warming. We can minimize the carbon footprint but we can also have a carbon handprint, a positive impact on the climate. Quality characteristics can be added with sustainability, and then measured continuously. Test environments can be used less, and in smaller scale and on demand. Test techniques can be used in optimizing or minimizing number of tests. Test automation can be used to speed up testing.
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
Generative AI Deep Dive: Advancing from Proof of Concept to ProductionAggregage
Join Maher Hanafi, VP of Engineering at Betterworks, in this new session where he'll share a practical framework to transform Gen AI prototypes into impactful products! He'll delve into the complexities of data collection and management, model selection and optimization, and ensuring security, scalability, and responsible use.
Elevating Tactical DDD Patterns Through Object CalisthenicsDorra BARTAGUIZ
After immersing yourself in the blue book and its red counterpart, attending DDD-focused conferences, and applying tactical patterns, you're left with a crucial question: How do I ensure my design is effective? Tactical patterns within Domain-Driven Design (DDD) serve as guiding principles for creating clear and manageable domain models. However, achieving success with these patterns requires additional guidance. Interestingly, we've observed that a set of constraints initially designed for training purposes remarkably aligns with effective pattern implementation, offering a more ‘mechanical’ approach. Let's explore together how Object Calisthenics can elevate the design of your tactical DDD patterns, offering concrete help for those venturing into DDD for the first time!
Welcome to the first live UiPath Community Day Dubai! Join us for this unique occasion to meet our local and global UiPath Community and leaders. You will get a full view of the MEA region's automation landscape and the AI Powered automation technology capabilities of UiPath. Also, hosted by our local partners Marc Ellis, you will enjoy a half-day packed with industry insights and automation peers networking.
📕 Curious on our agenda? Wait no more!
10:00 Welcome note - UiPath Community in Dubai
Lovely Sinha, UiPath Community Chapter Leader, UiPath MVPx3, Hyper-automation Consultant, First Abu Dhabi Bank
10:20 A UiPath cross-region MEA overview
Ashraf El Zarka, VP and Managing Director MEA, UiPath
10:35: Customer Success Journey
Deepthi Deepak, Head of Intelligent Automation CoE, First Abu Dhabi Bank
11:15 The UiPath approach to GenAI with our three principles: improve accuracy, supercharge productivity, and automate more
Boris Krumrey, Global VP, Automation Innovation, UiPath
12:15 To discover how Marc Ellis leverages tech-driven solutions in recruitment and managed services.
Brendan Lingam, Director of Sales and Business Development, Marc Ellis
1. Can DNA Barcoding help stop the budgie
smugglers? The future of barcoding as a
crime fighting tool in wildlife forensics
Rebecca N. Johnson, Andrew King, Robert Mason
Australian Museum Genetics Laboratory
iBOL November 28th 2011
rebecca.johnson@austmus.gov.au
4. Wildlife Forensic Science at the
Australian Museum - Sydney
• Systematics and
Biodiversity Research
• Conservation
genetics
• Population genetics
• Phylogenetics
• Wildlife Forensics
5. Wildlife forensic science –
What is it?
• Wildlife crime (flora, fauna)
• Management decisions
• Society for Wildlife Forensic Science-
Sept 2009
• Non-human forensic work in Australia is
not handled by law enforcement
authorities
9. The utility of DNA Barcoding for
Wildlife Forensic Science
• Voucher specimens, sequence
quality, assessment of variation
(intraspecific/interspecific)
• Standardized practices, protocols,
gene regions
10. The utility of DNA Barcoding for
Wildlife Forensic Science
CRollandi
Pbankanens
• Standardized practices –
Pvaiuli
combined with good taxonomy
58
99
unknown
Pbrachiali • Validated gene regions -
100
Aleucogast Probability:
99 Aaureus False –ve / False +ve
True –ve / True +ve
92
Prepom5a
96
Prepom5b
Ccyanea
• High node support value ->
100
78
Pomacentri
likelihood species match
Pcoelestis
10
11. The utility of DNA Barcoding for
Wildlife Forensic Science
• Voucher specimens, sequence
quality, assessment of variation
(intraspecific/interspecific)
• Standardized practices, protocols,
gene regions
• WF Science – a young discipline
12. Case Study: Fish tissue identification
2006 a Single case from NSW waters:
• 87 shark fins = MNI 22 sharks
• DNA analysis required for species ID
13. Australian Legislation – shark fins
http://www.dpiw.tas.gov.au/inter.nsf/WebPages/ALIR-4YB2L3?open#SharkFinRemoval
14. ‘Shark-finning’
• Removal of fins at sea and
torso discarded (can be alive)
• Indiscriminate taking of
species, size, gender and age
WPY 2010: “Desecration in Paradise” by Thomas Haider, Austria
15. Shark fin product
• Typically white cartilaginous
• Most expensive part of the animal
17. Case Study: Fish tissue identification
Single case from NSW waters:
• Guilty pleas
• Charges: Contravene condition of fishing
concession (Fisheries Management Act Cth
1991)
• Conviction recorded
• Fines of AUD$23,100
21. R v Della Zuana - Result
• The accused pleaded guilty to charges
of illegal importation (brought under the
Environment Protection and Biodiversity Conservation
Act, Cth 1999) for
import and trade in CITES
listed endangered species.
• The accused was sentenced to 2 years
in jail and $10,000 fine.
• He was deported upon release.
22. Can DNA Barcoding help stop the
budgie smugglers?
• A maturing discipline
• Standardised practises
• New techniques
• Deterrent - Cruel, costly, endangers
species’ survival, threatens biodiversity
23. Acknowledgements:
• Australian Museum: Cassie Vockler, Amanda Hay,
Mark McGrouther
• Australian Customs Service: Jeff Vandamme, Mick
O’Malley
• NSW Fisheries: Brendan Sinnett, Peter Chan
