Western Blot Antibody Customer Review for ADNP Polyclonal Antibody (STJ91502)St John's Laboratory Ltd
ADNP (Activity-Dependent Neuroprotector Homeobox) is expressed through the ADNP gene in humans. It is a possible transcription factor through having a homeobox and 9 zinc finger domains. It is said to have both stimulatory and inhibitory growth effects on different tumour cells. The ADNP Polyclonal Antibody detects endogenous levels of ADNP protein.
Western Blot Antibody Customer Review for ADNP Polyclonal Antibody (STJ91502)St John's Laboratory Ltd
ADNP (Activity-Dependent Neuroprotector Homeobox) is expressed through the ADNP gene in humans. It is a possible transcription factor through having a homeobox and 9 zinc finger domains. It is said to have both stimulatory and inhibitory growth effects on different tumour cells. The ADNP Polyclonal Antibody detects endogenous levels of ADNP protein.
This is nice presentation given by Vishal Goyani, an Analytical student of National Institute Of Pharmaceutical education and research-INDIA.
mail your query at - vngoyanii@gmail.com
Rapid DNA isolation from diverse plant material for use in Next Generation Se...QIAGEN
Isolation of DNA from plant material is often a tedious process which involves significant hands on time and leads to varying results due to the diverse nature of the material. Different parts of the plants as well as the plants themselves differ in both consistency of material and presence of inhibitory substances, making dependable isolation of DNA difficult.
Here, we developed a method for the efficient extraction of DNA from different plant types, including strawberry leaf, pine needle, grape leaf, and cotton and coffee seeds (workflow at right). A novel bead beating method and lysis chemistry led to more efficient sample lysis with minimal hands-on time and significantly increased DNA yield compared to conventional methods. Through the use of multiple technologies to improve removal of secondary metabolites, such as polyphenols, complex polysaccharides, alkaloids and tannins that may inhibit downstream applications, the isolated DNA was of high quality and purity.
The resulting DNA is suitable for immediate use in downstream reactions, including PCR, qPCR and Next Generation Sequencing based applications. Using this method we were further able to design a workflow that included DNA isolation, library preparation and bioinformatics analyses for the efficient detection of plant pathogens isolated from infected samples. With this, our protocol is a substantial improvement within workflows used for plant microbiome and plant pathology studies as well as in plant breeding and engineering.
Kupffer Cells Mediate Leptin-Induced Liver Fibrosis.
GASTROENTEROLOGY 2009;137:713–723
JIANHUA WANG,* ISABELLE LECLERCQ,‡ JOANNE M. BRYMORA,* NING XU,* MEHDI RAMEZANI–MOGHADAM,* ROSLYN M. LONDON,* DAVID BRIGSTOCK,§ and JACOB GEORGE*
*Storr Liver Unit, Westmead Millennium Institute, University of Sydney and Westmead Hospital, Westmead, Australia; ‡Laboratory of Gastroenterology, Faculty of
Medicine, Université Catholique de Louvain, Brussels, Belgium; and §Center for Cell and Vascular Biology, Children’s Research Institute, Columbus, Ohio
瘦素(Leptin)是一由脂肪細胞(Adipocyte)所分泌之荷爾蒙,是調控體重及新陳代謝之重要因子。過去研究發現病態肥胖(Obese)、脂肪肝(Nonalcoholic steatohepatitis)及酒精性肝炎(Alcoholic liver disease)等病患之血液循環中,Leptin量有明顯增加。而近期研究報告指出leptin具有促進肝臟纖維化(Liver fibrosis)之能力,當中分子機理並未明確。
在肝纖維化過程中,肝臟星狀細胞(HSC)會被活化增生及促進胞外基質(ECM)產生,而鄰近之Kupffer細胞(KC)則已知可透過促發炎因子(Proinflammatory factor)和促纖維化因子(Profibrogenic factors)例如TGF-β1和ROS影響HSC表現。雖然HSC是肝纖維化過程中重要角色,前人研究卻發現leptin似對HSC無任何調控作用。故本篇作者針對Leptin是否透過間接作用於HSC鄰近之KC,刺激其產生促纖維化因子,以活化HSC。
為探討leptin直接或間接影響HSC之分子機理,本篇作者透過RT-PCR、Immunoblot等分子生物學方法,分別測定leptin刺激後HSC及KC中Collagen I、TIMP1等促纖維化因子基因及蛋白表現,發現leptin雖可促使HSC增生,但對其纖維化能力之影響甚微。而leptin可刺激KC中TGF-β1及CTGF/CCN2等肝纖維化中重要之cytokines表現。另發現Leptin-treated KC-conditioned培養液可刺激HSC增生及增加其中Collagen I、TIMP1等表現,得出了leptin是透過刺激KC來活化HSC之推論。作者亦於後續實驗中,透過磷酸化測定、EMSA等方法探討leptin訊號傳遞作用,發現leptin可活化KC中STAT3、ERK1/2、AKT等路徑,及下游因子AP-1、NF-κB,而此兩種蛋白具有增強TGF-β1及CTGF/CCN2基因表現之能力。
SuperScript IV Reverse Transcriptase for RNA Analysis | ESHG 2015 Poster PS14...Thermo Fisher Scientific
Survey and interview studies conducted over a three year period revealed that researchers are not satisfied with their current reverse transcriptase and are performing reactions with increasingly difficult samples, such as poorly purified RNA and unpurified RNA (direct RT) that both contain inhibitors. To meet this performance gap, the Thermo Fisher Life Sciences Solutions group produced a new reverse transcriptase, SuperScript® IV, and experiments we performed show that it is the most robust reverse transcriptase compared to other enzymes. SuperScript® IV characterization was performed in the context of “real world” situations where users do not have perfect RNA samples. In the presence of a variety of inhibitors, we demonstrate that SuperScript® IV possesses superior performance in a variety of inhibitors, such as alcohols, salts, detergents, phenol, heparin, hematin, bile salts, and formalin typically found in sample preparation reagents, cell lines, blood, feces, and FFPE samples. This enzyme can even detect RNA targets in unpurified RNA samples (directly lysed cells) and whole blood without sacrificing sensitivity and yield. The introduction of SuperScript® IV enables researchers to obtain more consistent results independent of sample quality and simplify and speed up workflows by eliminating RNA purification.
Cell culture is the process by which prokaryotic, eukaryotic or plant cells are grown under controlled conditions. Mammalian cell culture technology has become a major field in modern biotechnology; mammalian cell culture refers to the cells of a mammalian, isolated from specific tissues (i.e. skin, liver, glands, etc.) and further cultivated and reproduced in an artificial medium. Cell culture technology is currently playing a major role in toxicity testing, cancer research, virology, genetic engineering, and gene therapy.
OBJECTIVE:
To observe the transfection of CHO and HEK cells with GFP
To observe the recombinant GFP using Western Blotting
To purify the transfected HEK and CHO cells using AKTA Pure Purification
Recombinant Fab fragments specific for the pfHRPII and pfHSP72 : implications for malaria diagnosis - Parole de junior de la 7e édition du Cours international « Atelier Paludisme » - Ravaoarisoa Elisabeth - Madagascar - elisa@pasteur.mg
Australia with gold coast, cairns and sydneywww.Tripmart.comtripmart
Australia Holidays - Book Australia Tours & travel packages at Tripmart. Largest number of Australia Tour & holiday Packages available. Go for a Hoilday, travel to Australia and its various tourist attractions with Australia holiday packages. Explore Australia Tourism with cheap vacation packages.
Switzerland Holidays - Book Switzerland Tours & travel packages at Tripmart. Largest number of Switzerland Tour & holiday Packages available. Go for a Hoilday, travel to Switzerland and its various tourist attractions with Switzerland holiday packages. Explore Switzerland Tourism with cheap vacation packages.
This is nice presentation given by Vishal Goyani, an Analytical student of National Institute Of Pharmaceutical education and research-INDIA.
mail your query at - vngoyanii@gmail.com
Rapid DNA isolation from diverse plant material for use in Next Generation Se...QIAGEN
Isolation of DNA from plant material is often a tedious process which involves significant hands on time and leads to varying results due to the diverse nature of the material. Different parts of the plants as well as the plants themselves differ in both consistency of material and presence of inhibitory substances, making dependable isolation of DNA difficult.
Here, we developed a method for the efficient extraction of DNA from different plant types, including strawberry leaf, pine needle, grape leaf, and cotton and coffee seeds (workflow at right). A novel bead beating method and lysis chemistry led to more efficient sample lysis with minimal hands-on time and significantly increased DNA yield compared to conventional methods. Through the use of multiple technologies to improve removal of secondary metabolites, such as polyphenols, complex polysaccharides, alkaloids and tannins that may inhibit downstream applications, the isolated DNA was of high quality and purity.
The resulting DNA is suitable for immediate use in downstream reactions, including PCR, qPCR and Next Generation Sequencing based applications. Using this method we were further able to design a workflow that included DNA isolation, library preparation and bioinformatics analyses for the efficient detection of plant pathogens isolated from infected samples. With this, our protocol is a substantial improvement within workflows used for plant microbiome and plant pathology studies as well as in plant breeding and engineering.
Kupffer Cells Mediate Leptin-Induced Liver Fibrosis.
GASTROENTEROLOGY 2009;137:713–723
JIANHUA WANG,* ISABELLE LECLERCQ,‡ JOANNE M. BRYMORA,* NING XU,* MEHDI RAMEZANI–MOGHADAM,* ROSLYN M. LONDON,* DAVID BRIGSTOCK,§ and JACOB GEORGE*
*Storr Liver Unit, Westmead Millennium Institute, University of Sydney and Westmead Hospital, Westmead, Australia; ‡Laboratory of Gastroenterology, Faculty of
Medicine, Université Catholique de Louvain, Brussels, Belgium; and §Center for Cell and Vascular Biology, Children’s Research Institute, Columbus, Ohio
瘦素(Leptin)是一由脂肪細胞(Adipocyte)所分泌之荷爾蒙,是調控體重及新陳代謝之重要因子。過去研究發現病態肥胖(Obese)、脂肪肝(Nonalcoholic steatohepatitis)及酒精性肝炎(Alcoholic liver disease)等病患之血液循環中,Leptin量有明顯增加。而近期研究報告指出leptin具有促進肝臟纖維化(Liver fibrosis)之能力,當中分子機理並未明確。
在肝纖維化過程中,肝臟星狀細胞(HSC)會被活化增生及促進胞外基質(ECM)產生,而鄰近之Kupffer細胞(KC)則已知可透過促發炎因子(Proinflammatory factor)和促纖維化因子(Profibrogenic factors)例如TGF-β1和ROS影響HSC表現。雖然HSC是肝纖維化過程中重要角色,前人研究卻發現leptin似對HSC無任何調控作用。故本篇作者針對Leptin是否透過間接作用於HSC鄰近之KC,刺激其產生促纖維化因子,以活化HSC。
為探討leptin直接或間接影響HSC之分子機理,本篇作者透過RT-PCR、Immunoblot等分子生物學方法,分別測定leptin刺激後HSC及KC中Collagen I、TIMP1等促纖維化因子基因及蛋白表現,發現leptin雖可促使HSC增生,但對其纖維化能力之影響甚微。而leptin可刺激KC中TGF-β1及CTGF/CCN2等肝纖維化中重要之cytokines表現。另發現Leptin-treated KC-conditioned培養液可刺激HSC增生及增加其中Collagen I、TIMP1等表現,得出了leptin是透過刺激KC來活化HSC之推論。作者亦於後續實驗中,透過磷酸化測定、EMSA等方法探討leptin訊號傳遞作用,發現leptin可活化KC中STAT3、ERK1/2、AKT等路徑,及下游因子AP-1、NF-κB,而此兩種蛋白具有增強TGF-β1及CTGF/CCN2基因表現之能力。
SuperScript IV Reverse Transcriptase for RNA Analysis | ESHG 2015 Poster PS14...Thermo Fisher Scientific
Survey and interview studies conducted over a three year period revealed that researchers are not satisfied with their current reverse transcriptase and are performing reactions with increasingly difficult samples, such as poorly purified RNA and unpurified RNA (direct RT) that both contain inhibitors. To meet this performance gap, the Thermo Fisher Life Sciences Solutions group produced a new reverse transcriptase, SuperScript® IV, and experiments we performed show that it is the most robust reverse transcriptase compared to other enzymes. SuperScript® IV characterization was performed in the context of “real world” situations where users do not have perfect RNA samples. In the presence of a variety of inhibitors, we demonstrate that SuperScript® IV possesses superior performance in a variety of inhibitors, such as alcohols, salts, detergents, phenol, heparin, hematin, bile salts, and formalin typically found in sample preparation reagents, cell lines, blood, feces, and FFPE samples. This enzyme can even detect RNA targets in unpurified RNA samples (directly lysed cells) and whole blood without sacrificing sensitivity and yield. The introduction of SuperScript® IV enables researchers to obtain more consistent results independent of sample quality and simplify and speed up workflows by eliminating RNA purification.
Cell culture is the process by which prokaryotic, eukaryotic or plant cells are grown under controlled conditions. Mammalian cell culture technology has become a major field in modern biotechnology; mammalian cell culture refers to the cells of a mammalian, isolated from specific tissues (i.e. skin, liver, glands, etc.) and further cultivated and reproduced in an artificial medium. Cell culture technology is currently playing a major role in toxicity testing, cancer research, virology, genetic engineering, and gene therapy.
OBJECTIVE:
To observe the transfection of CHO and HEK cells with GFP
To observe the recombinant GFP using Western Blotting
To purify the transfected HEK and CHO cells using AKTA Pure Purification
Recombinant Fab fragments specific for the pfHRPII and pfHSP72 : implications for malaria diagnosis - Parole de junior de la 7e édition du Cours international « Atelier Paludisme » - Ravaoarisoa Elisabeth - Madagascar - elisa@pasteur.mg
Australia with gold coast, cairns and sydneywww.Tripmart.comtripmart
Australia Holidays - Book Australia Tours & travel packages at Tripmart. Largest number of Australia Tour & holiday Packages available. Go for a Hoilday, travel to Australia and its various tourist attractions with Australia holiday packages. Explore Australia Tourism with cheap vacation packages.
Switzerland Holidays - Book Switzerland Tours & travel packages at Tripmart. Largest number of Switzerland Tour & holiday Packages available. Go for a Hoilday, travel to Switzerland and its various tourist attractions with Switzerland holiday packages. Explore Switzerland Tourism with cheap vacation packages.
The Evolution of Laboratory Data Systems: Replacing Paper, Streamlining Proce...IDBS
The laboratory has become an increasingly electronic environment. It’s not just that the volume of data is greater than ever before, it’s also being generated at ever-increasing speeds. As companies move towards a fully integrated lab environment there are benefits and pitfalls along the way. Successful projects start with a solid foundation, and keep a clear vision in mind.
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Translational Genomics and Prostate Cancer: Meet the NGS Experts Series Part 2QIAGEN
Advanced prostate cancer is highly heterogeneous but this inter-patient heterogeneity has until recently not been understood. We have through an international research effort dissected the molecular landscape of advanced castration resistant prostate, elucidating key molecular targets in this group of diseases. We have also shown that PARP inhibitors have antitumor activity against a significant proportion of these cancers, mainly in men whose cancers harbor DNA repair defects.
Dr. Robert Langer - Simposio Internacional 'Terapias oncológicas avanzadas'Fundación Ramón Areces
Los días 15 y 16 de octubre de 2014, la Fundación Ramón Areces y la Real Academia Nacional de Farmacia, en colaboración con la Fundación de la Innovación Bankinter, reunieron en Madrid a algunos de los mayores expertos mundiales en nuevas terapias contra el cáncer. El Simposio Internacional, coordinado por la profesora y académica María José Alonso, analizó el momento actual de la lucha contra esta enfermedad. También fue un punto de encuentro para científicos de los más innovadores institutos de investigación en oncología, quienes debatieron sobre tres grandes temas: la Medicina Personalizada contra el cáncer, los nanomedicamentos en la terapia del cáncer y las terapias basadas en la inmunomodulación.
Learning Objectives:
Describe the consequences of hyper- and hypovolemia for surgical and critically ill patients.
Develop a fluid management strategy for individual patients
High Throughput Investigation of EC Coupling in Isolated Cardiac MyocytesInsideScientific
During this webinar sponsored by IonOptix, Michiel Helmes, PhD discusses recent advancements in instrumentation that address the shortfalls of low throughput EC coupling characterization. Specifically, Dr. Helmes explains the technology behind faster data acquisition and analysis, as well as improvements to the studies that offer more data acquisition fidelity, and automated data collection. He offers insights into best-practices for proper EC coupling measurement and highlight improvements to data handling, namely faster, automated data analysis.
Background: Measuring and analyzing calcium and contractility in isolated cardiomyocytes offers important insights into cardiac function. However, traditional methods of obtaining EC coupling data are somewhat limited to lower throughput — for many applications, particularly drug discovery research, this presents a big challenge. Additionally, low throughput data acquisition and analysis may lack the statistical power necessary to fully resolve differences, or changes, in cardiac function. Isolated myocytes can behave heterogeneously, thus greater sample numbers are essential for accurate and reliable modeling of cardiac behavior.
The Future of Metabolic Phenotyping Using data bandwidth to maximize N, analy...InsideScientific
Methods matter. In metabolic measurement, confidence in reproducible results relies heavily on the design of the system used to acquire data. In the field of translational metabolic and behavioral phenotyping there is critical demand for more – throughput, standardization, synchronization of diverse data streams, temporal resolution, efficiency of workflow, and verification of results. We compare continuous and switched metabolic measurement methodologies and explore applications that benefit most from continuous measurement.
In this exclusive webinar sponsored by Sable Systems International, experts contrast methodologies and discuss how to improve best practices in metabolic phenotyping. We show how advances in high-bandwidth metabolic measurement, as implemented in Promethion metabolic phenotyping systems, leverage a 60- to 1200-fold increase in temporal resolution and achieve synchrony with intake and other behavioral data.
Key Topics:
* Time-saving methodologies for increasing throughput in multiplexed or continuous metabolic phenotyping
* Evaluation criteria for selecting a metabolic measurement system
* How the home-cage advantage of a pull-mode system reliably increases animal safety while dramatically reducing stress on both the animal and the researcher
* How to improve the resolution, accuracy and versatility of metabolic data using water vapor measurement
* The importance of raw data retention in metabolic phenotyping
* How deep data field format leads to greater traceability, improved reliability and far greater data extraction versatility to address research objectives
* How exact metabolic costs can be assigned to transient activities, with important implications for studies of energy balance, obesity, drug kinetics and metabolic diseases
An introduction to the use of ICP-MS in the clinical setting, that goes on to describe some potential new application areas for advanced instrumentation such as HPLC-ICP-MS, laser ablation-ICP-MS and immuno-tagging-ICP-MS for the measurement of biomolecules.
Similar to Peritoneal Dialysis And Secondary Renal Function (20)
2. Background/Goals
End Stage Renal Disease (ESRD)
Current therapy:
- Peritoneal Dialysis vs. Hemodialysis
- Few kidneys available for transplantation
Development of a viable renal function
replacement system
3. Methods
Animal model of uremia: Bilateral nephrectomies 45-
60 kg female sheep performed in two surgical
stages.
Stage 1
Left nephrectomy + PD catheters placement (infusion and removal)
Stage 2
Right nephrectomy, lines placed in carotid artery and jugular vein (to
monitor and control hemodynamics).
PD and BREC-d was turned on 24 hrs after second
surgery.
Data was collected up to 10 days.
4. Data Collection
Hemodynamics:
Heart Rate
Mean Arterial Pressure
Central Venous Pressure
Respiratory Rate
Electrolytes :
K+
Renal function:
BUN / CREAT
PD Fluid Flow Rate
5. Roller BREC-d F-40
pump
F-80 Trilogy
OUT
MC3 Trilogy
pump
Waste PD IN
6. Study Groups
Control (n=5)
Acellular BREC-d
Study - Cellular (n=11): Renal Epithelial Cells
Lamb BREC-d (n=6)
Human BREC-d (n=5)
7. Sheep Number BREC-d Cell type Duration of Study
Study 3 Lamb 10 days
5 Lamb 10 days
Groups 6 Lamb 10 days
7 Lamb 7 days
8 Lamb <1 day
9 Lamb 8 days
11 Human <1 day
12 Human 10 days
16 Human 10 days
17 Human 10 days
18 Human 10 days
20 Acellular 2.5 days
21 Acellular 5 days
22 Acellular 10 days
23 Acellular 7 days
24 Acellular 10 days
15. Metabolic Waste
BUN (Blood Urea Nitrogen)
Natural bi-product of metabolic function, can
cause damage to tissue if not excreted.
Value greater than 60mg/dl indicates severe
renal impairment
Creatine- Another waste product produce
through metabolism with an average range
of 0.6-1.5 mg/dL
16. Average PD Flow per Treatment Group
Error bars = SEM
Acellular-BREC (n=5) Cellular-BREC (n=11)
140
120
100
mL/min
80
60
40
20
0
0 24 48 72 96 120 144 168 192 216 240
Experimental Time (Hrs)
17. Sheep 18 Metabolic Waste
90
80
70
60
50
mg/dl
40
30
20
10
0
Pre Pre Brecs Brecs Day Brecs Day Brecs Day Final
Surgery 1 Day 0 1 2 3
BUN mg/dL (Reference Range 5.-20.) Creatine mg/dL (Reference Range 0.6-1.5)
18. Comparative BUN and Creatine
Data
Due to constant recirculation of PD fluid, metabolic
wastes such as blood urea nitrogen (BUN) and creatine
reach elevated levels.
Recirculation occurs so that renal cells can be kept alive
Thus PD effectiveness is significantly reduced
BREC-d does not replace primary functions, therefore no
effect on metabolites.
19. Viability of Renal Cell System
Oxygen Consumption in vivo
Animal Oxygen Consumption of
Recovered Renal Cells
Sheep 16 49.76 +/- 3.25 mmoles of Oxygen/ min
Sheep 17 35.88+/- 7.86 mmoles of Oxygen/ min
Sheep 18 33.08 +/- 4.18 mmoles of Oxygen/ min
Average oxygen consumption in vitro is 19.24 mmoles of
Oxygen/ min
This data illustrates that renal cells can survive in
BREC-d environment.
20. Conclusions
Cellular BREC-d maintains acceptable MAP.
Respiration rate elevated
Discomfort due to dialysate volume
More PD fluid exchanges necessary to better
control K+; BUN; CREAT
Renal cells are viable for course of study.
21. Future Research
More effective PD fluid
Analyze effects of BREC-d unit for longer period
time (>2 weeks)
Analyze sheep vs human BREC-d
Increase frequency of PD fluid exchange
Twice a day (am/pm)