Cv i chiao lee 2016
•
0 likes•233 views
I-Chiao Lee is a molecular microbiologist from Taiwan with over 10 years of experience studying probiotics. She received her PhD from Wageningen University in the Netherlands, where she focused on surface molecules of probiotics, including proteins, polysaccharides, and teichoic acids. Her work has resulted in several publications examining ways to improve the survival and expression of beneficial molecules in probiotic bacteria like Lactobacillus plantarum. She is skilled in a variety of techniques and enjoys working independently and creatively in a friendly environment.
Report
Share
Report
Share
Download to read offline
Recommended
Bioteknologi Bab 6 Materi IPAkelompok hendriko SMPN 264 Jakarta
This document discusses biotechnology and provides definitions, examples of conventional vs modern biotechnology, benefits in various fields such as agriculture, medicine, environment and social applications. It also outlines some positive and negative impacts of biotechnology development, including increased crop yields but also environmental damage from chemical fertilizers, and international trade competition issues.
Bab 6 Bioteknologi kelas 9G SMPN 264 Jakarta
This document discusses biotechnology and is divided into three sections. The first section defines biotechnology and explains that it uses various sciences like microbiology and molecular biology. The second section describes examples of conventional biotechnology like tempeh production and modern biotechnology techniques like genetic engineering. The third section discusses the benefits of biotechnology in health, agriculture, and animal farming but also notes some biotechnology products may pose health risks if cleanliness is not maintained.
2.F Virus
The document discusses the taxonomy and classification of viruses. It begins with an overview of the early history of virology from the 18th century discoveries of Edward Jenner and Dimitri Ivanowski to the 20th century developments in virus isolation, cultivation, and structure determination. It then covers the key topics of virus properties, cultivation methods, purification and assays, structure, and the principles of virus taxonomy established by the International Committee on Taxonomy of Viruses. The taxonomy is based on attributes like nucleic acid type, strandedness, host, and presence or absence of an envelope.
VIABLE BUT NON-CULTURABLE BACTERIA- VBNC
VBNC cells enter a dormant, viable but non-culturable state in response to harsh environmental conditions such as nutrient starvation, temperature fluctuations, and osmotic stress. While metabolically active at a low level, VBNC cells cannot be cultured with standard techniques but maintain integrity and the potential to regain culturability. VBNC states have been observed in pathogenic bacteria like Vibrio cholerae and allow survival outside a host until conditions become suitable for regrowth. Detection of VBNC cells requires techniques beyond traditional culturing, such as fluorescent microscopy or PCR-based methods.
Cell Culture Media PPT
Cell culture media can be natural or artificial. Natural media include coagula/clots, biological fluids, and tissue extracts. Artificial (synthetic) media include serum-containing, serum-free, and chemically defined media. Serum-containing media supplement defined media like Eagle Minimum Essential Medium with 5-20% serum, which provides nutrients, hormones, growth factors, and other important components to support cell growth. However, serum can also inhibit some cell types and contain cytotoxic constituents. Serum-free media avoid these disadvantages but may be specific to cell types and require close control of conditions. Chemically defined media use ultrapure inorganic and organic components and defined protein additives produced through genetic engineering.
2009. yeast
Yeasts are eukaryotic unicellular fungi that reproduce asexually through budding or fission. The article discusses the classification, ecology, cell structure, nutrition, and importance of yeasts. It focuses on Saccharomyces cerevisiae, the most widely used yeast in food and industry. Yeasts play roles in various habitats and the food chain, and have potential for uses in biotechnology.
Single Cell Proteins
This document discusses single cell proteins (SCP) which are dried microbial cells used as a source of protein for humans and animals. It provides background on SCP, describing how they can help address protein deficiency and meet global protein demand. The document outlines various microorganisms used for SCP production such as algae, fungi, bacteria and yeast. It discusses their protein content, substrates used, and factors considered for large scale production of SCP. The nutritional value and potential health benefits of SCP are also summarized.
Tissue culture basic techniques
Plant tissue culture involves growing plant cells, tissues or organs in an artificial nutrient medium under sterile conditions. It requires sterilized explants, culture medium containing appropriate nutrients, growth regulators and gelling agents. The basic steps are sterilization of explants, inoculation and culturing under appropriate light and temperature. Subculturing is done to produce more plantlets. Differentiation and organogenesis produces shoots, roots or callus based on growth regulator levels. Hardening acclimatizes tissue culture raised plantlets to field conditions. Benefits include mass propagation of plants, improving traits and producing disease free plants.
Recommended
Bioteknologi Bab 6 Materi IPAkelompok hendriko SMPN 264 Jakarta
This document discusses biotechnology and provides definitions, examples of conventional vs modern biotechnology, benefits in various fields such as agriculture, medicine, environment and social applications. It also outlines some positive and negative impacts of biotechnology development, including increased crop yields but also environmental damage from chemical fertilizers, and international trade competition issues.
Bab 6 Bioteknologi kelas 9G SMPN 264 Jakarta
This document discusses biotechnology and is divided into three sections. The first section defines biotechnology and explains that it uses various sciences like microbiology and molecular biology. The second section describes examples of conventional biotechnology like tempeh production and modern biotechnology techniques like genetic engineering. The third section discusses the benefits of biotechnology in health, agriculture, and animal farming but also notes some biotechnology products may pose health risks if cleanliness is not maintained.
2.F Virus
The document discusses the taxonomy and classification of viruses. It begins with an overview of the early history of virology from the 18th century discoveries of Edward Jenner and Dimitri Ivanowski to the 20th century developments in virus isolation, cultivation, and structure determination. It then covers the key topics of virus properties, cultivation methods, purification and assays, structure, and the principles of virus taxonomy established by the International Committee on Taxonomy of Viruses. The taxonomy is based on attributes like nucleic acid type, strandedness, host, and presence or absence of an envelope.
VIABLE BUT NON-CULTURABLE BACTERIA- VBNC
VBNC cells enter a dormant, viable but non-culturable state in response to harsh environmental conditions such as nutrient starvation, temperature fluctuations, and osmotic stress. While metabolically active at a low level, VBNC cells cannot be cultured with standard techniques but maintain integrity and the potential to regain culturability. VBNC states have been observed in pathogenic bacteria like Vibrio cholerae and allow survival outside a host until conditions become suitable for regrowth. Detection of VBNC cells requires techniques beyond traditional culturing, such as fluorescent microscopy or PCR-based methods.
Cell Culture Media PPT
Cell culture media can be natural or artificial. Natural media include coagula/clots, biological fluids, and tissue extracts. Artificial (synthetic) media include serum-containing, serum-free, and chemically defined media. Serum-containing media supplement defined media like Eagle Minimum Essential Medium with 5-20% serum, which provides nutrients, hormones, growth factors, and other important components to support cell growth. However, serum can also inhibit some cell types and contain cytotoxic constituents. Serum-free media avoid these disadvantages but may be specific to cell types and require close control of conditions. Chemically defined media use ultrapure inorganic and organic components and defined protein additives produced through genetic engineering.
2009. yeast
Yeasts are eukaryotic unicellular fungi that reproduce asexually through budding or fission. The article discusses the classification, ecology, cell structure, nutrition, and importance of yeasts. It focuses on Saccharomyces cerevisiae, the most widely used yeast in food and industry. Yeasts play roles in various habitats and the food chain, and have potential for uses in biotechnology.
Single Cell Proteins
This document discusses single cell proteins (SCP) which are dried microbial cells used as a source of protein for humans and animals. It provides background on SCP, describing how they can help address protein deficiency and meet global protein demand. The document outlines various microorganisms used for SCP production such as algae, fungi, bacteria and yeast. It discusses their protein content, substrates used, and factors considered for large scale production of SCP. The nutritional value and potential health benefits of SCP are also summarized.
Tissue culture basic techniques
Plant tissue culture involves growing plant cells, tissues or organs in an artificial nutrient medium under sterile conditions. It requires sterilized explants, culture medium containing appropriate nutrients, growth regulators and gelling agents. The basic steps are sterilization of explants, inoculation and culturing under appropriate light and temperature. Subculturing is done to produce more plantlets. Differentiation and organogenesis produces shoots, roots or callus based on growth regulator levels. Hardening acclimatizes tissue culture raised plantlets to field conditions. Benefits include mass propagation of plants, improving traits and producing disease free plants.
Microbiological examination of food
The document discusses methods for examining food and water microbiologically. There are three main methods discussed: culture media methods, immunoassay methods, and polymerase chain reaction (PCR) methods. Culture media methods involve culturing samples on selective and differential media to isolate and identify microorganisms. Immunoassay methods like ELISA use antigen-antibody binding to detect toxins and microbes. PCR methods amplify and detect targeted nucleic acid sequences to identify pathogens. Examining food and water microbiologically is important to ensure safety and determine processing parameters needed to meet standards.
Apoptosis
The document discusses apoptosis and the immune system in silkworms infected with Staphylococcus aureus. It provides background on apoptosis, the silkworm life cycle, and S. aureus. The study aims to investigate the pathogenicity of S. aureus in silkworms by examining apoptosis and the host immune response. S. aureus is known to trigger apoptosis in other hosts, and the study will focus on this mechanism and immune system activation in silkworms using it as a model organism.
Bioteknologi pendahuluan
This document provides a timeline of key developments in biotechnology from 8000 BCE to 2012 CE. Some highlights include the domestication of crops and livestock in 8000-4000 BCE, the use of yeast for leavening bread and fermenting beer in 2000 BCE, the discovery of DNA's role in heredity in the mid-1800s and early 1900s, the development of genetic engineering and recombinant DNA techniques in the 1970s, the launch of the Human Genome Project in 1990, the cloning of Dolly the sheep in 1997, and the discovery that mature cells can be reprogrammed in 2012. The timeline traces the evolution of biotechnology from early agricultural practices to modern genetic research and applications in medicine.
Mikrobiologi - Penggolongan Mikroorganisme
The document discusses the classification of microorganisms. It begins by describing Archaea, dividing them into two phyla: Crenarchaeota and Euryarchaeota. It then covers the classification of bacteria based on Bergey's Manual, dividing them into four phyla according to cell wall characteristics. Many medically important bacteria are also described, including gram-negative rods in the families Enterobacteriaceae, Vibrionaceae, and Pasteurellaceae. Gram-negative cocci, spirochetes, rickettsias, chlamydias and other groups are also mentioned.
Introduction to Pharmaceutical microbiology
Microbiology is the study of microscopic organisms. Pharmaceutical microbiology applies these concepts to pharmaceutical operations. Some key areas where microbiology is important in pharmacy include pharmaceutical production, medical devices, and cosmetics. The history of microbiology began with Anton van Leeuwenhoek's discovery of microorganisms in the 1600s and important figures like Louis Pasteur, who disproved spontaneous generation, and Robert Koch, who linked specific bacteria to particular diseases. Microbiology has many applications including antibiotic production, enzyme production, sterilization, and testing of pharmaceuticals.
Introductions to scope of food and microbiology.doc
1. Microbiology is the study of microscopic organisms including bacteria, fungi, algae, protozoa, and viruses. It examines their structure, physiology, classification, and interactions with humans and other organisms.
2. Bacterial cells have a cell wall and plasma membrane, with some containing flagella, pili, or capsules. Their DNA is located in the nucleoid region. Gram staining distinguishes between Gram-positive and Gram-negative bacteria based on differences in cell wall composition.
3. Microscopy techniques like brightfield, fluorescence, electron, and phase contrast microscopy are used to observe cellular and subcellular structures at different magnifications. Staining enhances contrast for visualization.
General Characters Of Microorganisms
This document provides information about microorganisms and their classification. It discusses that microorganisms are very small organisms that cannot be seen with the naked eye, including bacteria, fungi, protozoa, algae, and viruses. It also describes the prokaryotic and eukaryotic cell structures, and explains how microorganisms are classified based on their shape, staining properties, nutritional requirements, temperature and pH tolerances, and oxygen requirements. The document highlights different types of microbial reproduction including binary fission.
Animal Cell culture by S.D.Mankar, Pravara Rural college of Pharmacy
Growth of animal cells in culture, general procedure for cell culture,
Primary, established and transformed cell cultures.
Application of cell cultures in pharmaceutical industry and research.
Microbilogy note
This document provides an overview of microbiology and the history of the field. It begins with an introduction to microbiology and explains key topics like the size and types of microorganisms. It then discusses the history of microbiology from early pioneers like Van Leeuwenhoek and discoveries around spontaneous generation and germ theory. Major developments are highlighted such as vaccines, staining techniques, and Koch's postulates. The modern areas of study and ongoing importance of microbiology are also summarized.
Cell Culture
The document discusses mammalian cell culture and microbiology. It provides information on:
1) The importance of cell culture for biotechnology and research applications like studying drug effects and genetically engineering bacteria.
2) The optimal conditions needed for cell growth, including the type of growth medium, temperature, pH, and aseptic techniques.
3) How microbial cells can be cultured on small or large scales for uses in food production, chemical synthesis, and more.
Microbiology lec1
The document provides an overview of microbiology and bacterial cell structure. It discusses that microbiology is the study of microorganisms too small to be seen with the naked eye. It then summarizes the different types of microorganisms studied in medical microbiology and branches of microbiology. Finally, it outlines the typical structures of a bacterial cell, including the cell wall, cell membrane, cytoplasm, capsules, pili, flagella, and their functions.
Bacteria growth and nutritional requirements.
Bacteria can reproduce through binary fission, budding, or fragmentation. During binary fission, a bacterial cell takes up nutrients, undergoes biosynthesis, and doubles its DNA and cell size before dividing into two daughter cells. Budding occurs when a small bud forms on the parent cell and separates to form a new cell. Fragmentation involves the breaking apart of filamentous bacteria into smaller cells. Bacteria also require nutrients for growth, with generation time referring to the time it takes for a single cell to divide into two daughter cells. Growth can be studied using batch, chemostat, or turbidostat cultures.
micro organisms
- Bacteria are unicellular microorganisms that exist in various shapes including spheres, rods, and spirals. They reproduce through binary fission and are found in medicine, food industries, and decomposing matter.
- Algae are simple autotrophic organisms ranging from unicellular to multicellular forms. They are usually found in aquatic or moist places and contain chlorophyll. Some algae include Spirogyra and kelp. Algal blooms can be harmful.
- Fungi are eukaryotic organisms in the kingdom Fungi. Examples include Rhizopus mold and edible Agaricus mushrooms. Fungi have useful and harmful aspects like food spoilage or production
7.Laboratory safety and Biohazard
Workplace safety is an important aspect to protect personnel against injury or serious accident.In case of animal cell culture safety takes a front seat due to nature of work i.e. handling of human cells and tissues, viruses with high potential to cause infections to humans and other adventitious micro organisms. This presentation presents various methods of safety to protect lab personnel from infectious biological agents.
Fermentation technology
Microorganisms are capable of growing on a wide range of substrates and can produce a remarkable spectrum of products. The relatively recent advent of in vitro genetic manipulation has extended the range of products that may be produced by microorganisms and has provided new methods for increasing the yields of existing ones. The commercial exploitation of the biochemical diversity of microorganisms has resulted in the development of the fermentation industry and the techniques of genetic manipulation have given this well-established industry the
opportunity to develop new processes and to improve existing ones. The term fermentation is derived from the Latin verb fervere, to boil, which describes the appearance of the action of yeast on extracts of fruit or malted grain during the production of alcoholic beverages. However, fermentation is interpreted differently by microbiologists and biochemists. To a microbiologist the word means any process for the production of a product by the mass culture of microorganisms. To a biochemist, however, the word means an energy-generating process in which organic compounds act as both electron donors and acceptors, that is, an anaerobic process where energy is produced without the participation of oxygen or other inorganic electron acceptors. In this chapter fermentation is used in its broader, microbiological context.
Probiotics and biofilms
This document provides an overview of probiotics and biofilms. It begins with definitions of probiotics, prebiotics, and synbiotics. The mechanisms of action of probiotics are then described, including anti-cancer, anti-diarrheal, immunomodulation, and anti-allergy effects. Biofilm formation, development, and dispersal are explained. Key properties of biofilms like their structure, quorum sensing, and antibiotic resistance are covered. The roles of biofilms in infectious diseases and examples like dental caries are highlighted. Control and removal of biofilms is also discussed.
History of microbial biotechnology
Microbial biotechnology uses microorganisms and their byproducts to develop technologies across various industries. It has applications in healthcare through products like antibiotics, vaccines, and diagnostics; agriculture through improved crops and bioremediation; and industry through fermentation to produce chemicals, compounds, and pharmaceuticals. The history of microbial biotechnology began in the 17th century with the first observations of microorganisms under a microscope. Pioneers like van Leeuwenhoek, Pasteur, Koch, and Waksman made discoveries that advanced the fields of microbiology and biotechnology through techniques like staining, sterilization, and the discovery of antibiotics. Industrial applications grew through the fermentation of products like alcohol, vinegar,
Introduction to Animal Cell Culture
This presentation presents an overview of definition, equipment, various cell culturing methods,
characterization, and applications of animal cell culture. This presentation also contains MCQs to acquaint reader about types of question asked in various competitive examinations.
microbiology branches.pptx
Microbiology is the study of microorganisms like bacteria, fungi and viruses. There are several branches of microbiology including bacteriology, mycology, virology and parasitology.
Bacteriology is the study of bacteria and their characteristics such as their cell structure, genetics and role in causing disease. Bacteria can be classified based on their shape, cell wall composition, mode of nutrition and respiration. They reproduce through binary fission.
Mycology is the study of fungi which have cell walls made of chitin. Fungi are classified based on their sexual reproduction and morphology. They can reproduce sexually through spores or asexually through budding or hyphal growth.
Viro
Detection of contaminants_in_human_cell_culture
This document outlines procedures for detecting various contaminants in human cell cultures, including microbes, fungi, viruses, and mycoplasma. It describes microscopic and macroscopic checks for initial detection of bacterial or fungal contamination. Detection of viral contamination involves using PCR or other techniques to test for pathogens like HIV, HBV, and EBV. Mycoplasma contamination is detected using one of two methods - a PCR-based test or a luminometric assay that detects mycoplasma enzyme activity. Periodic screening is important to ensure cell cultures are free of contamination.
Yuan kun lee
This document discusses the potential for developing localized probiotic formulations tailored to specific geographic regions and populations. It notes key differences in gut microbiota composition and dietary habits between regions that could impact probiotic efficacy. Specific needs in developing nations like vitamin production and diarrhea prevention are also addressed. The document advocates considering how probiotics might survive locally consumed foods and addresses technological advances that could aid rational probiotic selection based on regional diets and health issues.
Probiotics in Diabetes Treatment by Tariful
This document discusses probiotics and their potential role in treating diabetes. It begins by providing background on probiotics and how their use has increased in clinical studies over time. Probiotics have been shown to impact gut function, immunity, oral health and metabolic conditions like diabetes and obesity. The document then discusses how gut microbiota alterations from factors like diet and stress can increase intestinal permeability, activate the immune system and interrupt insulin activity, leading to diabetes. It proposes that probiotic treatment may help by producing substances like butyrate and GLP-1 that aid insulin secretion. The remainder outlines a process for developing a probiotic formulation to treat diabetes, from identifying potential probiotic bacteria to clinical trials. It emphasizes the importance of the gut
More Related Content
What's hot
Microbiological examination of food
The document discusses methods for examining food and water microbiologically. There are three main methods discussed: culture media methods, immunoassay methods, and polymerase chain reaction (PCR) methods. Culture media methods involve culturing samples on selective and differential media to isolate and identify microorganisms. Immunoassay methods like ELISA use antigen-antibody binding to detect toxins and microbes. PCR methods amplify and detect targeted nucleic acid sequences to identify pathogens. Examining food and water microbiologically is important to ensure safety and determine processing parameters needed to meet standards.
Apoptosis
The document discusses apoptosis and the immune system in silkworms infected with Staphylococcus aureus. It provides background on apoptosis, the silkworm life cycle, and S. aureus. The study aims to investigate the pathogenicity of S. aureus in silkworms by examining apoptosis and the host immune response. S. aureus is known to trigger apoptosis in other hosts, and the study will focus on this mechanism and immune system activation in silkworms using it as a model organism.
Bioteknologi pendahuluan
This document provides a timeline of key developments in biotechnology from 8000 BCE to 2012 CE. Some highlights include the domestication of crops and livestock in 8000-4000 BCE, the use of yeast for leavening bread and fermenting beer in 2000 BCE, the discovery of DNA's role in heredity in the mid-1800s and early 1900s, the development of genetic engineering and recombinant DNA techniques in the 1970s, the launch of the Human Genome Project in 1990, the cloning of Dolly the sheep in 1997, and the discovery that mature cells can be reprogrammed in 2012. The timeline traces the evolution of biotechnology from early agricultural practices to modern genetic research and applications in medicine.
Mikrobiologi - Penggolongan Mikroorganisme
The document discusses the classification of microorganisms. It begins by describing Archaea, dividing them into two phyla: Crenarchaeota and Euryarchaeota. It then covers the classification of bacteria based on Bergey's Manual, dividing them into four phyla according to cell wall characteristics. Many medically important bacteria are also described, including gram-negative rods in the families Enterobacteriaceae, Vibrionaceae, and Pasteurellaceae. Gram-negative cocci, spirochetes, rickettsias, chlamydias and other groups are also mentioned.
Introduction to Pharmaceutical microbiology
Microbiology is the study of microscopic organisms. Pharmaceutical microbiology applies these concepts to pharmaceutical operations. Some key areas where microbiology is important in pharmacy include pharmaceutical production, medical devices, and cosmetics. The history of microbiology began with Anton van Leeuwenhoek's discovery of microorganisms in the 1600s and important figures like Louis Pasteur, who disproved spontaneous generation, and Robert Koch, who linked specific bacteria to particular diseases. Microbiology has many applications including antibiotic production, enzyme production, sterilization, and testing of pharmaceuticals.
Introductions to scope of food and microbiology.doc
1. Microbiology is the study of microscopic organisms including bacteria, fungi, algae, protozoa, and viruses. It examines their structure, physiology, classification, and interactions with humans and other organisms.
2. Bacterial cells have a cell wall and plasma membrane, with some containing flagella, pili, or capsules. Their DNA is located in the nucleoid region. Gram staining distinguishes between Gram-positive and Gram-negative bacteria based on differences in cell wall composition.
3. Microscopy techniques like brightfield, fluorescence, electron, and phase contrast microscopy are used to observe cellular and subcellular structures at different magnifications. Staining enhances contrast for visualization.
General Characters Of Microorganisms
This document provides information about microorganisms and their classification. It discusses that microorganisms are very small organisms that cannot be seen with the naked eye, including bacteria, fungi, protozoa, algae, and viruses. It also describes the prokaryotic and eukaryotic cell structures, and explains how microorganisms are classified based on their shape, staining properties, nutritional requirements, temperature and pH tolerances, and oxygen requirements. The document highlights different types of microbial reproduction including binary fission.
Animal Cell culture by S.D.Mankar, Pravara Rural college of Pharmacy
Growth of animal cells in culture, general procedure for cell culture,
Primary, established and transformed cell cultures.
Application of cell cultures in pharmaceutical industry and research.
Microbilogy note
This document provides an overview of microbiology and the history of the field. It begins with an introduction to microbiology and explains key topics like the size and types of microorganisms. It then discusses the history of microbiology from early pioneers like Van Leeuwenhoek and discoveries around spontaneous generation and germ theory. Major developments are highlighted such as vaccines, staining techniques, and Koch's postulates. The modern areas of study and ongoing importance of microbiology are also summarized.
Cell Culture
The document discusses mammalian cell culture and microbiology. It provides information on:
1) The importance of cell culture for biotechnology and research applications like studying drug effects and genetically engineering bacteria.
2) The optimal conditions needed for cell growth, including the type of growth medium, temperature, pH, and aseptic techniques.
3) How microbial cells can be cultured on small or large scales for uses in food production, chemical synthesis, and more.
Microbiology lec1
The document provides an overview of microbiology and bacterial cell structure. It discusses that microbiology is the study of microorganisms too small to be seen with the naked eye. It then summarizes the different types of microorganisms studied in medical microbiology and branches of microbiology. Finally, it outlines the typical structures of a bacterial cell, including the cell wall, cell membrane, cytoplasm, capsules, pili, flagella, and their functions.
Bacteria growth and nutritional requirements.
Bacteria can reproduce through binary fission, budding, or fragmentation. During binary fission, a bacterial cell takes up nutrients, undergoes biosynthesis, and doubles its DNA and cell size before dividing into two daughter cells. Budding occurs when a small bud forms on the parent cell and separates to form a new cell. Fragmentation involves the breaking apart of filamentous bacteria into smaller cells. Bacteria also require nutrients for growth, with generation time referring to the time it takes for a single cell to divide into two daughter cells. Growth can be studied using batch, chemostat, or turbidostat cultures.
micro organisms
- Bacteria are unicellular microorganisms that exist in various shapes including spheres, rods, and spirals. They reproduce through binary fission and are found in medicine, food industries, and decomposing matter.
- Algae are simple autotrophic organisms ranging from unicellular to multicellular forms. They are usually found in aquatic or moist places and contain chlorophyll. Some algae include Spirogyra and kelp. Algal blooms can be harmful.
- Fungi are eukaryotic organisms in the kingdom Fungi. Examples include Rhizopus mold and edible Agaricus mushrooms. Fungi have useful and harmful aspects like food spoilage or production
7.Laboratory safety and Biohazard
Workplace safety is an important aspect to protect personnel against injury or serious accident.In case of animal cell culture safety takes a front seat due to nature of work i.e. handling of human cells and tissues, viruses with high potential to cause infections to humans and other adventitious micro organisms. This presentation presents various methods of safety to protect lab personnel from infectious biological agents.
Fermentation technology
Microorganisms are capable of growing on a wide range of substrates and can produce a remarkable spectrum of products. The relatively recent advent of in vitro genetic manipulation has extended the range of products that may be produced by microorganisms and has provided new methods for increasing the yields of existing ones. The commercial exploitation of the biochemical diversity of microorganisms has resulted in the development of the fermentation industry and the techniques of genetic manipulation have given this well-established industry the
opportunity to develop new processes and to improve existing ones. The term fermentation is derived from the Latin verb fervere, to boil, which describes the appearance of the action of yeast on extracts of fruit or malted grain during the production of alcoholic beverages. However, fermentation is interpreted differently by microbiologists and biochemists. To a microbiologist the word means any process for the production of a product by the mass culture of microorganisms. To a biochemist, however, the word means an energy-generating process in which organic compounds act as both electron donors and acceptors, that is, an anaerobic process where energy is produced without the participation of oxygen or other inorganic electron acceptors. In this chapter fermentation is used in its broader, microbiological context.
Probiotics and biofilms
This document provides an overview of probiotics and biofilms. It begins with definitions of probiotics, prebiotics, and synbiotics. The mechanisms of action of probiotics are then described, including anti-cancer, anti-diarrheal, immunomodulation, and anti-allergy effects. Biofilm formation, development, and dispersal are explained. Key properties of biofilms like their structure, quorum sensing, and antibiotic resistance are covered. The roles of biofilms in infectious diseases and examples like dental caries are highlighted. Control and removal of biofilms is also discussed.
History of microbial biotechnology
Microbial biotechnology uses microorganisms and their byproducts to develop technologies across various industries. It has applications in healthcare through products like antibiotics, vaccines, and diagnostics; agriculture through improved crops and bioremediation; and industry through fermentation to produce chemicals, compounds, and pharmaceuticals. The history of microbial biotechnology began in the 17th century with the first observations of microorganisms under a microscope. Pioneers like van Leeuwenhoek, Pasteur, Koch, and Waksman made discoveries that advanced the fields of microbiology and biotechnology through techniques like staining, sterilization, and the discovery of antibiotics. Industrial applications grew through the fermentation of products like alcohol, vinegar,
Introduction to Animal Cell Culture
This presentation presents an overview of definition, equipment, various cell culturing methods,
characterization, and applications of animal cell culture. This presentation also contains MCQs to acquaint reader about types of question asked in various competitive examinations.
microbiology branches.pptx
Microbiology is the study of microorganisms like bacteria, fungi and viruses. There are several branches of microbiology including bacteriology, mycology, virology and parasitology.
Bacteriology is the study of bacteria and their characteristics such as their cell structure, genetics and role in causing disease. Bacteria can be classified based on their shape, cell wall composition, mode of nutrition and respiration. They reproduce through binary fission.
Mycology is the study of fungi which have cell walls made of chitin. Fungi are classified based on their sexual reproduction and morphology. They can reproduce sexually through spores or asexually through budding or hyphal growth.
Viro
Detection of contaminants_in_human_cell_culture
This document outlines procedures for detecting various contaminants in human cell cultures, including microbes, fungi, viruses, and mycoplasma. It describes microscopic and macroscopic checks for initial detection of bacterial or fungal contamination. Detection of viral contamination involves using PCR or other techniques to test for pathogens like HIV, HBV, and EBV. Mycoplasma contamination is detected using one of two methods - a PCR-based test or a luminometric assay that detects mycoplasma enzyme activity. Periodic screening is important to ensure cell cultures are free of contamination.
What's hot (20)
Introductions to scope of food and microbiology.doc
Introductions to scope of food and microbiology.doc
Animal Cell culture by S.D.Mankar, Pravara Rural college of Pharmacy
Animal Cell culture by S.D.Mankar, Pravara Rural college of Pharmacy
Similar to Cv i chiao lee 2016
Yuan kun lee
This document discusses the potential for developing localized probiotic formulations tailored to specific geographic regions and populations. It notes key differences in gut microbiota composition and dietary habits between regions that could impact probiotic efficacy. Specific needs in developing nations like vitamin production and diarrhea prevention are also addressed. The document advocates considering how probiotics might survive locally consumed foods and addresses technological advances that could aid rational probiotic selection based on regional diets and health issues.
Probiotics in Diabetes Treatment by Tariful
This document discusses probiotics and their potential role in treating diabetes. It begins by providing background on probiotics and how their use has increased in clinical studies over time. Probiotics have been shown to impact gut function, immunity, oral health and metabolic conditions like diabetes and obesity. The document then discusses how gut microbiota alterations from factors like diet and stress can increase intestinal permeability, activate the immune system and interrupt insulin activity, leading to diabetes. It proposes that probiotic treatment may help by producing substances like butyrate and GLP-1 that aid insulin secretion. The remainder outlines a process for developing a probiotic formulation to treat diabetes, from identifying potential probiotic bacteria to clinical trials. It emphasizes the importance of the gut
Almere dietisten en mondhygienisten 15 06-2018
This document discusses the relationship between the human gastrointestinal microbiota, diet, and health conditions like diabetes. It notes that the microbiota plays an important role in nutrient absorption and production, complementing human biochemistry. A healthy microbiota is characterized by homeostasis and resilience after disturbances. Studies on fecal microbiota transplantation have shown potential for treating obesity and diabetes by altering the microbiota composition. The oral and small intestine microbiota are also discussed, and their connection through swallowing. Certain genera like Streptococcus and Veillonella are common to both sites.
BIOFILM FORMATION
this ppt deals with biofilm formation mechanism y microbes produce it and how it affects humans in a brief manner
2 (2011) the role of gut micriobiota and the mucosal barrier in the pathogene...
This document summarizes research on the role of gut microbiota and the mucosal barrier in inflammatory and autoimmune diseases and cancer. It discusses how germ-free and gnotobiotic animal models that are free of microbes or colonized with specific microbes have provided insights. Studies using these models have shown that gut microbiota components can directly influence chronic intestinal inflammation and colon cancer development. Additionally, microbiota colonization protects against autoimmune diabetes in mice. The document also reviews the composition and function of the gut microbiota and mucosal barrier, which involves mucus, epithelial cells, antimicrobial peptides, and immune cells that help maintain homeostasis and prevent pathogen invasion.
Tempeh & probiotics
Temph production & compositon
microbes involve in temph production
probiotics: composition, uses, microbes , advantage ,benifits
Microbiota intestinale e Patologie Neurodegenerative
Presentazione a cura del Professor Giovanni Gasbarrini - XII° Congresso Nazionale FIMeG 2018 - The Silver Tsunami: l'anziano fra appropriatezza e farmaeconomia
Food microbiology notes
This document discusses the microbiology of various foods. It begins by introducing food microbiology and the importance of microorganisms in foods, both desirable and undesirable roles. It then discusses the microbiology of specific foods, including milk and milk products like cheese, butter, and ice cream. It describes the microorganisms commonly found in milk, how they grow, and their effects. It provides details on the microbiology of butter, sources of contamination, types of spoilage, and control methods. It also briefly mentions the microbiology of cheese and cottage cheese.
Presentation1
This document discusses probiotics and their health benefits. It defines probiotics as live microbes formulated into foods, drugs, and supplements. Probiotics are believed to promote health by inhibiting potentially pathogenic microorganisms in the gut through several mechanisms like reducing intestinal pH, producing bacteriocins, and competing for nutrients and adhesion sites. The document also discusses prebiotics, which are non-digestible fibers that nourish beneficial gut bacteria, and synbiotics, which are combinations of probiotics and prebiotics that have combined effects. Common probiotic microbes used are lactic acid bacteria which convert sugars into lactic acid, lowering pH.
Dr Alfio Comis CV 2017
This document provides a summary of the education and professional experience of Dr. Alfio Comis. It outlines his roles as CEO and Managing Scientist of various companies focused on monoclonal antibody production. It also lists his areas of expertise in protein biochemistry, hybridoma technology, and monoclonal antibody production. Finally, it provides an overview of his teaching philosophy and lists publications, graduate students supervised, and past research funding.
Probiotics by Akash Raza
The document discusses probiotics, which are living microorganisms that provide health benefits when consumed in adequate amounts. It provides background on the origin of probiotics, examples of probiotic microorganisms like Lactobacillus and Bifidobacterium, their global use and health effects such as aiding digestion and boosting immunity. It also discusses prebiotics, synbiotics, current issues around probiotic products and who can benefit from consuming probiotics.
Probiotics1 12766811034852-phpapp01
The document discusses probiotics, which are living microorganisms that provide health benefits when consumed in adequate amounts. It provides background on the origin of probiotics, examples of probiotic microorganisms like Lactobacillus and Bifidobacterium, their global use and health effects such as aiding digestion and boosting immunity. It also discusses prebiotics, synbiotics, current issues around probiotic products and who can benefit from consuming probiotics.
Silvia Alonso
"Metagenomics in food safety: what’s the
added value? Case studies from the livestock
sector in Tanzania and Uganda"
Biofilms
This document discusses biofilms in endodontic infections. It begins with definitions of biofilms and describes their ultrastructure, composition, and stages of formation. It then discusses the basic criteria for biofilms including autopoiesis, homeostasis, synergy, and communality. The document outlines the characteristics of biofilms including their protection of bacteria and enhanced tolerance to antimicrobials. It also discusses different types of endodontic biofilms and microorganisms involved in their formation. Methods to study and quantify biofilms are described.
Antibacterial potential of probiotics. Thesis defense presentation
This document summarizes a study assessing the antibacterial properties of probiotics against foodborne pathogens. The study isolated probiotic strains from infant feces and duck guts, including various Bifidobacterium and Lactobacillus species. Agar well diffusion assays found that certain strains inhibited the growth of Shigella and E. coli, with the highest activity from L. plantarum and B. animalis. The antibacterial agents in probiotic extracts were found to be heat stable. While acidity contributed to activity, other factors were also involved. The study provides insights into using probiotics to prevent infections and highlights opportunities to better understand their antimicrobial mechanisms of action.
Bruno pot
This document summarizes a talk on probiotics and mucosal immunity interactions in early life. It discusses how the microbiota changes throughout life, from pregnancy and birth through adulthood. Signals from the environment, including probiotics, shape immune development during critical windows. While some probiotic studies show benefits, effects depend on many factors and probiotics alone may not be sufficient. The microbiota plays an important role in immune maturation, and disturbances could impact health through epigenetic changes. Careful consideration of the microbiota is needed when evaluating human development and health.
Cell culture practices-I(Media preparation,cell.pptx
A growth medium or culture medium is a liquid or gel designated to support the growth of microorganisms,cells,or small plants.
Culture media generally comprise an appropriate sourcde of energy and compounds which regulate the cell cycle.
A typical culture media is composed of a complement of amino acids,vitamins,inorganic salts,glucose, and serum as a source of growth factors, hormones, and attachment factors.
In addition to nutrients, the medium also helps maintain pH and osmolarity.
Imporatant amino acids, trace elements, growth factor,hormone,transport protein and adhesion factor are added.
Adhesion factor added are main components of intercellular substance such as fibronectin,collagen and laminin.
Primary purpose of introducing SFM is to promote the specific growth of a particular type of cell.
Exapmple of one serum free media: DCMM-1 SFM. High protein serum free medium, designed for hybridoma cell growth and monoclonal antibody production.
Probiotics ppt
This document presents information on probiotics. It begins by defining probiotics as live microorganisms that provide health benefits when consumed. The most common probiotic bacteria are lactobacilli and bifidobacteria. Probiotics are important for human health as they help maintain a balanced microbiome and protect against antibiotic resistance. They work by producing inhibitory compounds, competing for nutrients, and enhancing immune response. Probiotics must be able to survive the digestive system and colonize the intestines to provide health benefits. They are used to treat various clinical conditions such as diarrhea and inflammatory bowel disease.
Probiotics
Probiotics are living microorganisms that provide health benefits when consumed in adequate amounts. They include lactic acid bacteria like Lactobacillus and Bifidobacterium species. Probiotics can survive the harsh conditions of the gastrointestinal tract, adhere to intestinal cells, produce antimicrobial substances, and modulate the immune system to help crowd out pathogenic bacteria. While generally safe, some risks include mild digestive side effects, infection in immunocompromised individuals, and potential transfer of antibiotic resistance genes. More research is still needed to substantiate health claims and develop targeted "designer" probiotic strains.
Similar to Cv i chiao lee 2016 (20)
identification of new microorganisms with probiotic potential and.pptx
identification of new microorganisms with probiotic potential and.pptx
2 (2011) the role of gut micriobiota and the mucosal barrier in the pathogene...
2 (2011) the role of gut micriobiota and the mucosal barrier in the pathogene...
Microbiota intestinale e Patologie Neurodegenerative
Microbiota intestinale e Patologie Neurodegenerative
Antibacterial potential of probiotics. Thesis defense presentation
Antibacterial potential of probiotics. Thesis defense presentation
Cell culture practices-I(Media preparation,cell.pptx
Cell culture practices-I(Media preparation,cell.pptx
Recently uploaded
8.Isolation of pure cultures and preservation of cultures.pdf
Isolation of pure culture, its various method.
HUMAN EYE By-R.M Class 10 phy best digital notes.pdf
Class 10 human eye notes physics
Handwritten best quality
Gadgets for management of stored product pests_Dr.UPR.pdf
Insectsplayamajorroleinthedeteriorationoffoodgrainscausingbothquantitativeandqualitativelosses
Wellprovedthatnogranariescanbefilledwithgrainswithoutinsectsastheharvestedproducecontainegg(or)larvae(or)pupae(or)adultinsectinthembecauseoffieldcarryoverinfestationwhichcannotbeavoidedindevelopingcountrieslikeIndia
Simpletechnologiesfortimelydetectionofinsectsinthestoredproduceandtherebyplantimelycontrolmeasures
Authoring a personal GPT for your research and practice: How we created the Q...
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
The debris of the ‘last major merger’ is dynamically young
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
MICROBIAL INTERACTION PPT/ MICROBIAL INTERACTION AND THEIR TYPES // PLANT MIC...
MICROBIAL INTERACTION PPT/ MICROBIAL INTERACTION AND THEIR TYPES // PLANT MIC...ABHISHEK SONI NIMT INSTITUTE OF MEDICAL AND PARAMEDCIAL SCIENCES , GOVT PG COLLEGE NOIDA
Microbial interaction
Microorganisms interacts with each other and can be physically associated with another organisms in a variety of ways.
One organism can be located on the surface of another organism as an ectobiont or located within another organism as endobiont.
Microbial interaction may be positive such as mutualism, proto-cooperation, commensalism or may be negative such as parasitism, predation or competition
Types of microbial interaction
Positive interaction: mutualism, proto-cooperation, commensalism
Negative interaction: Ammensalism (antagonism), parasitism, predation, competition
I. Mutualism:
It is defined as the relationship in which each organism in interaction gets benefits from association. It is an obligatory relationship in which mutualist and host are metabolically dependent on each other.
Mutualistic relationship is very specific where one member of association cannot be replaced by another species.
Mutualism require close physical contact between interacting organisms.
Relationship of mutualism allows organisms to exist in habitat that could not occupied by either species alone.
Mutualistic relationship between organisms allows them to act as a single organism.
Examples of mutualism:
i. Lichens:
Lichens are excellent example of mutualism.
They are the association of specific fungi and certain genus of algae. In lichen, fungal partner is called mycobiont and algal partner is called
II. Syntrophism:
It is an association in which the growth of one organism either depends on or improved by the substrate provided by another organism.
In syntrophism both organism in association gets benefits.
Compound A
Utilized by population 1
Compound B
Utilized by population 2
Compound C
utilized by both Population 1+2
Products
In this theoretical example of syntrophism, population 1 is able to utilize and metabolize compound A, forming compound B but cannot metabolize beyond compound B without co-operation of population 2. Population 2is unable to utilize compound A but it can metabolize compound B forming compound C. Then both population 1 and 2 are able to carry out metabolic reaction which leads to formation of end product that neither population could produce alone.
Examples of syntrophism:
i. Methanogenic ecosystem in sludge digester
Methane produced by methanogenic bacteria depends upon interspecies hydrogen transfer by other fermentative bacteria.
Anaerobic fermentative bacteria generate CO2 and H2 utilizing carbohydrates which is then utilized by methanogenic bacteria (Methanobacter) to produce methane.
ii. Lactobacillus arobinosus and Enterococcus faecalis:
In the minimal media, Lactobacillus arobinosus and Enterococcus faecalis are able to grow together but not alone.
The synergistic relationship between E. faecalis and L. arobinosus occurs in which E. faecalis require folic acid
Evidence of Jet Activity from the Secondary Black Hole in the OJ 287 Binary S...
Wereport the study of a huge optical intraday flare on 2021 November 12 at 2 a.m. UT in the blazar OJ287. In the binary black hole model, it is associated with an impact of the secondary black hole on the accretion disk of the primary. Our multifrequency observing campaign was set up to search for such a signature of the impact based on a prediction made 8 yr earlier. The first I-band results of the flare have already been reported by Kishore et al. (2024). Here we combine these data with our monitoring in the R-band. There is a big change in the R–I spectral index by 1.0 ±0.1 between the normal background and the flare, suggesting a new component of radiation. The polarization variation during the rise of the flare suggests the same. The limits on the source size place it most reasonably in the jet of the secondary BH. We then ask why we have not seen this phenomenon before. We show that OJ287 was never before observed with sufficient sensitivity on the night when the flare should have happened according to the binary model. We also study the probability that this flare is just an oversized example of intraday variability using the Krakow data set of intense monitoring between 2015 and 2023. We find that the occurrence of a flare of this size and rapidity is unlikely. In machine-readable Tables 1 and 2, we give the full orbit-linked historical light curve of OJ287 as well as the dense monitoring sample of Krakow.
Discovery of An Apparent Red, High-Velocity Type Ia Supernova at 𝐳 = 2.9 wi...
We present the JWST discovery of SN 2023adsy, a transient object located in a host galaxy JADES-GS
+
53.13485
−
27.82088
with a host spectroscopic redshift of
2.903
±
0.007
. The transient was identified in deep James Webb Space Telescope (JWST)/NIRCam imaging from the JWST Advanced Deep Extragalactic Survey (JADES) program. Photometric and spectroscopic followup with NIRCam and NIRSpec, respectively, confirm the redshift and yield UV-NIR light-curve, NIR color, and spectroscopic information all consistent with a Type Ia classification. Despite its classification as a likely SN Ia, SN 2023adsy is both fairly red (
�
(
�
−
�
)
∼
0.9
) despite a host galaxy with low-extinction and has a high Ca II velocity (
19
,
000
±
2
,
000
km/s) compared to the general population of SNe Ia. While these characteristics are consistent with some Ca-rich SNe Ia, particularly SN 2016hnk, SN 2023adsy is intrinsically brighter than the low-
�
Ca-rich population. Although such an object is too red for any low-
�
cosmological sample, we apply a fiducial standardization approach to SN 2023adsy and find that the SN 2023adsy luminosity distance measurement is in excellent agreement (
≲
1
�
) with
Λ
CDM. Therefore unlike low-
�
Ca-rich SNe Ia, SN 2023adsy is standardizable and gives no indication that SN Ia standardized luminosities change significantly with redshift. A larger sample of distant SNe Ia is required to determine if SN Ia population characteristics at high-
�
truly diverge from their low-
�
counterparts, and to confirm that standardized luminosities nevertheless remain constant with redshift.
Applied Science: Thermodynamics, Laws & Methodology.pdf
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
11.1 Role of physical biological in deterioration of grains.pdf
Storagedeteriorationisanyformoflossinquantityandqualityofbio-materials.
Themajorcausesofdeteriorationinstorage
•Physical
•Biological
•Mechanical
•Chemical
Storageonlypreservesquality.Itneverimprovesquality.
Itisadvisabletostartstoragewithqualityfoodproduct.Productwithinitialpoorqualityquicklydepreciates
在线办理(salfor毕业证书)索尔福德大学毕业证毕业完成信一模一样
学校原件一模一样【微信：741003700 】《(salfor毕业证书)索尔福德大学毕业证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Immersive Learning That Works: Research Grounding and Paths Forward
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
Recently uploaded (20)
Microbiology of Central Nervous System INFECTIONS.pdf
Microbiology of Central Nervous System INFECTIONS.pdf
8.Isolation of pure cultures and preservation of cultures.pdf
8.Isolation of pure cultures and preservation of cultures.pdf
HUMAN EYE By-R.M Class 10 phy best digital notes.pdf
HUMAN EYE By-R.M Class 10 phy best digital notes.pdf
Gadgets for management of stored product pests_Dr.UPR.pdf
Gadgets for management of stored product pests_Dr.UPR.pdf
Authoring a personal GPT for your research and practice: How we created the Q...
Authoring a personal GPT for your research and practice: How we created the Q...
The debris of the ‘last major merger’ is dynamically young
The debris of the ‘last major merger’ is dynamically young
MICROBIAL INTERACTION PPT/ MICROBIAL INTERACTION AND THEIR TYPES // PLANT MIC...
MICROBIAL INTERACTION PPT/ MICROBIAL INTERACTION AND THEIR TYPES // PLANT MIC...
Evidence of Jet Activity from the Secondary Black Hole in the OJ 287 Binary S...
Evidence of Jet Activity from the Secondary Black Hole in the OJ 287 Binary S...
Discovery of An Apparent Red, High-Velocity Type Ia Supernova at 𝐳 = 2.9 wi...
Discovery of An Apparent Red, High-Velocity Type Ia Supernova at 𝐳 = 2.9 wi...
Juaristi, Jon. - El canon espanol. El legado de la cultura española a la civi...
Juaristi, Jon. - El canon espanol. El legado de la cultura española a la civi...
Tissue fluids_etiology_volume regulation_pressure.pptx
Tissue fluids_etiology_volume regulation_pressure.pptx
Applied Science: Thermodynamics, Laws & Methodology.pdf
Applied Science: Thermodynamics, Laws & Methodology.pdf
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
11.1 Role of physical biological in deterioration of grains.pdf
11.1 Role of physical biological in deterioration of grains.pdf
Immersive Learning That Works: Research Grounding and Paths Forward
Immersive Learning That Works: Research Grounding and Paths Forward
Cv i chiao lee 2016
- 1. LINKEDIN E-MAIL ichiaolee@gmail.com MOBILE +31 6 8179 9105 I-CHIAO LEE EXPERIENCE An experienced molecular microbiologist, who is independent and responsible. Like to work in a well organized manner with a creative mind. Perform the best in a friendly environment. PhD CANDIDATE 2011 - 2016 Wageningen University The Netherlands INTERNSHIP 2010 Genetwister Technologies The Netherlands MASTER THESIS 2009 - 2010 TIFN Project NIZO Food Research The Netherlands RESEARCH ASSISTANT 2006 - 2008 Academia Sinica IBMS Taiwan Focus on surface molecules, including proteins, CPS, and teichoic acids, of probiotics Study biochemical, physiological and immunological properties of probiotic molecules Focus on identifying molecular markers for apple ripening using RNA isolation and qRT-PCR techniques Study the diversity of ripening within an apple tree Focus on in vitro gastrointestinal tract survival of probiotics Identified a regulator improving the survival of Lactobacillus plantarum WCFS1 EDUCATION MSc BIOTECHNOLOGY 2008 - 2010 BSc LIFE SCIENCES 2001 - 2005 Wageningen University, The Netherlands Specialized in molecular and cell biotechnology Graduated with “cum laude” National Taiwan University, Taiwan Obtain solid knowledge in biology, genetics, biochemistry, microbiology and molecular biology Focus on improving surface protein expression stability and quantity and on modifying enzyme specificity using laboratory evolution Skilled with mammalian cell tissue culture techniques LANGUAGES SKILLS INTERESTS CHINESE (Native) ENGLISH (Fluent) DUTCH (Basic A2) CREATIVE LOGICAL REASONING INDEPENDENT TENNIS and SWIMMING PAINTING and POTTERY BAKING and COOKING
- 2. PUBLICATION MICROBIOLOGY published ahead of print: 17 Dec 2015 JOURNAL OF BACTERIOLOGY 196 (2014) 9 p. 1671 - 1682 PHARMACOLOGICAL RESEARCH 69 (2013) 1 p. 61 – 74 PLoS ONE 7 (2012) 7 e39053 PLoS ONE 8 (2013) 9 e75084 GtfA and GtfB are both required for protein O- glycosylation in Lactobacillus plantarum I-Chiao Lee, Iris I van Swam, Satoru Tomita, Pierre Morsomme, Thomas Rolain, Pascal Hols, Michiel Kleerebezem, Peter A Bron The quest for probiotic effector molecules— Unraveling strain specificity at the molecular level I-Chiao Lee, Satoru Tomita, Michiel Kleerebezem, Peter A. Bron Modulation of Lactobacillus plantarum Gastrointestinal Robustness by Fermentation Conditions Enables Identification of Bacterial Robustness Markers Hermien van Bokhorst-van de Veen, I-Chiao Lee, Maria L Marco, Michiel Wels, Peter A Bron, Michiel Kleerebezem The B7-1 Cytoplasmic Tail Enhances Intracellular Transport and Mammalian Cell Surface Display of Chimeric Proteins in the Absence of a Linear ER Export Motif Yi-Chieh Lin, Bing-Mae Chen, Wei-Cheng Lu, Chien-I Su, Zeljko M Prijovich, Wen-Chuan Chung, Pei-Yu Wu, Kai-Chuan Chen, Ting- Yi Juan, I-Chiao Lee, Steve R Roffler PATENT WO2012150861 A1 2012 Probiotics with enhanced survival properties Hermien VAN BOKHORST-VAN DE VEEN, I-Chiao LEE, Michiel Wouter Wilhelmus WELS, Paulus DE VOS, Peter Allard BRON, Roger Samuel BONGERS, Anne WIERSMA, Michiel KLEEREBEZEM I-CHIAO LEE Born on 21 FEBRUARY 1983 in TAIPEI, TAIWAN Live now in Lombardi 70, 6708LT Wageningen, The Netherlands Characterisation of the transcriptional regulation of the tarIJKL-locus involved in ribitol-containing wall teichoic acid biosynthesis in Lactobacillus plantarum Satoru Tomita, I-Chiao Lee, Iris I van Swam, Sjef Boeren, Jacques Vervoort, Peter A Bron, Michiel Kleerebezem References Prof. dr. Michiel Kleerebezem michiel.kleerebezem@wur.nl Dr. ir. Peter A Bron peter.bron@nizo.com