Chloroplasts are organelles found in plant cells and algae that conduct photosynthesis. They contain their own DNA known as the chloroplast genome, which is typically 100-200kb in size and encodes genes for photosynthesis. The chloroplast genome is highly conserved and maternally inherited. It has been used for phylogenetic studies and shows potential for genetic engineering due to high transgene expression and maternal inheritance that prevents gene flow to other species.
Chromatin is the complex combination of DNA and proteins that makes up chromosomes. It can be made visible by staining with specific techniques and stain (thus the name chromatin which literally means colored material). The major proteins involved in chromatin are histone proteins; although many other chromosomal proteins have prominent roles too. The functions of chromatin is to package DNA into smaller volume to fit in the cell, to strengthen the DNA to allow mitosis and meiosis and to serve as a mechanism to control gene expression and DNA replication.
Prokaryotic and eukaryotic gene structurestusharamodugu
Organization of genome in Prokaryotes:
The term prokaryote means “primitive nucleus”. Cell in prokaryotes have no nucleus. The prokaryotic chromosome is dispersed within the cell and is not enclosed by a separate membrane. Much of the information about the structure of DNA comes from studies of prokaryotes, because they are less complex than eukaryotes. Prokaryotes are monoploids they have only one set of genes (one copy of the genome). In most viruses and prokaryotes, the single set of genes is stored in a single chromosome (single molecule either RNA or DNA).
Organization of genome in Prokaryotes:
The term prokaryote means “primitive nucleus”. Cell in prokaryotes have no nucleus. The prokaryotic chromosome is dispersed within the cell and is not enclosed by a separate membrane. Much of the information about the structure of DNA comes from studies of prokaryotes, because they are less complex than eukaryotes. Prokaryotes are monoploids they have only one set of genes (one copy of the genome). In most viruses and prokaryotes, the single set of genes is stored in a single chromosome (single molecule either RNA or DNA). Organization of genome in Prokaryotes:
The term prokaryote means “primitive nucleus”. Cell in prokaryotes have no nucleus. The prokaryotic chromosome is dispersed within the cell and is not enclosed by a separate membrane. Much of the information about the structure of DNA comes from studies of prokaryotes, because they are less complex than eukaryotes. Prokaryotes are monoploids they have only one set of genes (one copy of the genome). In most viruses and prokaryotes, the single set of genes is stored in a single chromosome (single molecule either RNA or DNA).
Chromatin is the complex combination of DNA and proteins that makes up chromosomes. It can be made visible by staining with specific techniques and stain (thus the name chromatin which literally means colored material). The major proteins involved in chromatin are histone proteins; although many other chromosomal proteins have prominent roles too. The functions of chromatin is to package DNA into smaller volume to fit in the cell, to strengthen the DNA to allow mitosis and meiosis and to serve as a mechanism to control gene expression and DNA replication.
Prokaryotic and eukaryotic gene structurestusharamodugu
Organization of genome in Prokaryotes:
The term prokaryote means “primitive nucleus”. Cell in prokaryotes have no nucleus. The prokaryotic chromosome is dispersed within the cell and is not enclosed by a separate membrane. Much of the information about the structure of DNA comes from studies of prokaryotes, because they are less complex than eukaryotes. Prokaryotes are monoploids they have only one set of genes (one copy of the genome). In most viruses and prokaryotes, the single set of genes is stored in a single chromosome (single molecule either RNA or DNA).
Organization of genome in Prokaryotes:
The term prokaryote means “primitive nucleus”. Cell in prokaryotes have no nucleus. The prokaryotic chromosome is dispersed within the cell and is not enclosed by a separate membrane. Much of the information about the structure of DNA comes from studies of prokaryotes, because they are less complex than eukaryotes. Prokaryotes are monoploids they have only one set of genes (one copy of the genome). In most viruses and prokaryotes, the single set of genes is stored in a single chromosome (single molecule either RNA or DNA). Organization of genome in Prokaryotes:
The term prokaryote means “primitive nucleus”. Cell in prokaryotes have no nucleus. The prokaryotic chromosome is dispersed within the cell and is not enclosed by a separate membrane. Much of the information about the structure of DNA comes from studies of prokaryotes, because they are less complex than eukaryotes. Prokaryotes are monoploids they have only one set of genes (one copy of the genome). In most viruses and prokaryotes, the single set of genes is stored in a single chromosome (single molecule either RNA or DNA).
Extranuclear inheritance or cytoplasmic inheritance is the transmission of genes that occur outside the nucleus. It is found in most eukaryotes and is commonly known to occur in cytoplasmic organelles such as mitochondria and chloroplasts or from cellular parasites like viruses or bacteria. Determining the contribution of organelle genes to plant phenotype is hampered by several factors, including the paucity of variation in the plastid and mitochondrial genomes. Mitochondria are organelles which function to transform energy as a result of cellular respiration. Chloroplasts are organelles which function to produce sugars via photosynthesis in plants and algae. The genes located in mitochondria and chloroplasts are very important for proper cellular function, yet the genomes replicate independently of the DNA located in the nucleus, which is typically arranged in chromosomes that only replicate one time preceding cellular division. The extranuclear genomes of mitochondria and chloroplasts however replicate independently of cell division. They replicate in response to a cell's increasing energy needs which adjust during that cell's lifespan. There is consistent difference between the results from reciprocal crosses; generally only the trait from female parent is transmitted. In most cases, there is no segregation in the F2 and subsequent generations.
Plant genetic engineering is one of the key technologies for crop improvement as well as an emerging approach for producing recombinant proteins in plants. Both plant nuclear and plastid genomes can be genetically modified, yet fundamental functional differences between the eukaryotic genome of the plant cell nucleus and the prokaryotic-like genome of the plastid will have an impact on key characteristics of the resulting transgenic organism. So, which genome, nuclear or plastid, to transform for the desired transgenic phenotype? In this paper we compare the advantages and drawbacks of engineering plant nuclear and plastid genomes to generate transgenic plants with the traits of interest, and evaluate the pros and cons of their use for different biotechnology and basic research applications. The chloroplast is a pivotal organelle in plant cells and eukaryotic algae to carry out photosynthesis, which provides the primary source of the world’s food. The expression of foreign genes in chloroplasts offers several advantages over their expression in the nucleus: high-level expression, no position effects, no vector sequences allowing stable transgene expression. In addition, transgenic chloroplasts are generally not transmitted through pollen grains because of the cytoplasmic localization. In the past two decades, great progress in chloroplast engineering has been made.
Plant Chromosomes: European Cytogeneticists outline: Trude Schwarzacher and P...Pat (JS) Heslop-Harrison
An overview of plant molecular cytogenetics. The lecture Trude Schwarzacher presented to the ECA conference Strasbourg in July 2015 is http://www.slideshare.net/PatHeslopHarrison/trude-schwarzacher
Molecular Systematics provides a solid conceptual basis for the evolutionary history of organisms. Molecular systematics is the study of DNA and RNA sequences to infer evolutionary links across organisms. Molecular approaches/ techniques provide excellent resources for the study of evolution and phylogeny.
A transplastomic plant is a genetically modified plant in which the new genes have not been inserted in the nuclear DNA but in the DNA of the chloroplasts.
Comparative genomics in eukaryotes, organellesKAUSHAL SAHU
WHAT IS COMPARATIVE GENOMICS?
HISTORY
SOME RELATED TERMS
MINIMAL EUKARYOTIC GENOMES
COMPARISON OF THE MAJOR SEQUENCED GENOMES
EUKARYOTIC GENOMES
SACCHAROMYCES CEREVISIAE GENOME
INSECT GENOME
DROSOPHILA MELANOGASTER (FRUIT FLY) GENOME
COMPARATIVE ANALYSIS OF THE HUMAN AND MOUSE GENOME
COMPARATIVE GENOMICS OF ORGANELLES
COMPARATIVE GENOMICS TOOLS
CONCLUSION
REFERENCES
A number of developments have been made in the molecular biology of oat (Avena spp.) in recent years. Many of these were recently described at the Fourth International Oat Conference, held on 18 to 23 October, in Adelaide, South Australia. These advances include a report of oat transformation and regeneration, the characterisation of J3-glucanase genes in oat, the further development of a molecular genetic map in oats, and the characterisation of genes encoding novel oat grain proteins. A technique for assessing pedigrees in the oat and other cereal crops has been reported using a modified electrophoretic technique.
chloroplast being the second semi-autonomous organelle of the plant cell also harbours its genome. the presentation includes various characteristic features of this organelle genome along with its functional pecularities and significance
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
5. - organelle found in plant cells and eukaryotic
algae
- The main role of chloroplasts is to
conduct photosynthesis, where the photosynthetic
pigment chlorophyll captures
the energy from sunlight and converts it and stores it
in the energy-storage
molecules ATP and NADPH while
freeing oxygen from water
- Photosynthesis.
IntroductionIntroduction:-:-
Chloroplast:
6. Abstract:
Chloroplasts play a crucial role in sustaining life on earth.
The availability of over 800 sequenced chloroplast genomes from a
variety of land plants has enhanced our understanding of chloroplast
biology, intracellular gene transfer, conservation, diversity, and the
genetic basis by which chloroplast transgenes can be engineered to
enhance plant agronomic traits or to produce high-value agricultural or
biomedical products.
In this review, we discuss the impact of chloroplast genome
sequences on understanding the origins of economically important
cultivated species and changes that have taken place during
domestication.
10. - Chloroplast DNA (cpDNA) is also known as plastid
DNA (ptDNA).
- Circular double stranded DNA molecule
- Chloroplast genome size ranges 120-217kb with
majority of plants fall into 120-160kb. (Pelargonium
has a chloroplast genome size 217kb)
- contain about 100 genes to synthesize proteins
- cpDNA regions includes Large Single-Copy (LSC)
& Small Single-Copy (SSC) regions, and Inverted
Repeats (IRA & IRB).
- Conifers and a group of legumes lack Inverted
Repeats.
Chloroplast genomeChloroplast genome
12. - Complete chloroplast DNA sequences of four land
plants (Nicotiana tabacum, Marchantia polymorpha,
Oryza sativa and Epifagus virginiana) were available
for comparative study on structure and gene content
of chloroplast genomes in 1980s.
- At present, the number of complete chloroplast
genome sequences is 122 (from 114 different
organisms).
eg. Arabidopsis thaliana, Coffea arabica, Eucalyptus
globulus, Glycine max, Gossypium hirsutum,
Helianthus annuus, Lycopersicon esculentum,
Nymphaea alba, Phaseolus vulgaris, Pinus koraiensis,
Piper cenocladum, Solanum tuberosum, Triticum
aestivum, Vitis vinifera, Zea mays etc.
Chloroplast genomeChloroplast genome
13. - cpDNA is a relatively abundant component of
plant total DNA, thus facilitating extraction
and analysis.
- Conservative rate of nucleotide substitution
enables to resolve plant phylogenetic
relationships at deep levels of evolution.
eg. familial level; mono- & dicotyledonous;
- Chloroplast protein-coding genes evolve at a
rate that is on average fivefold slower than
plant nuclear genes.
Characteristics of Chloroplast GenomeCharacteristics of Chloroplast Genome
14. - Strictly maternally inherited in most
angiosperms while in conifers, inheritance
is paternal.
- Chloroplast DNA is passed on from one
generation to the next with only an
occasional mutation altering the molecule;
sexual recombination does not occur.
15. - cpDNA regions can be amplified by means
of PCR.
- The resulted PCR products may be
subjected to RFLP or DNA sequencing.
- Common cpDNA regions used in
systematic study:
rbcL (1400bp), trnL-trnF (250-800bp), atpB-
rbcL (1000bp), trnL intron (300bp), matK
(2600bp), trnT-trnL (400-800bp), 16S (1400bp),
rpoC (3600bp) etc.
Molecular Systematics on cpDNAMolecular Systematics on cpDNA
17. - Restriction site mapping of
the entire chloroplast
genome. (involve the
isolation of chloroplast
DNA from the total DNA)
Molecular Systematics on cpDNAMolecular Systematics on cpDNA
The whole chloroplast genomes of
different Brassica species were
digested with SacI
18. - Singular structural rearrangements (e.g.
inversions and intron losses).
- Loss of intron of rpl2 gene was found in
species of order Caryophyllales (cacti,
amaranths, carnations, carnivorous plants).
Molecular Systematics on cpDNAMolecular Systematics on cpDNA
19. - On of the most comprehensive phylogenetic
study of cpDNA rearrangement involved a
22kb inversion found to be shared by 57
genera representing all tribes of the family
Asteraceae (sunflowers), a large plant family
with 20,000 species and 1100 genera.
- 50kb inversion brought psbA closer to rbcL in
legumes.
- 25kb inversion brought atpA closer to rbcL in
wheat.
Molecular Systematics on cpDNAMolecular Systematics on cpDNA
20. H. nervosa
H. dyeri
H. dryobalanoides
H. beccariana
H. pierrei
H. latifolia
H. mengerawan
H. myrtifolia
H. ferruginea
H. sangal
H. nutans
H. odorata
H. helferi
H. apiculata
H. wightiana
Neobalanocarpus heimii
69
84
89
100
75
5192
Subsection
Hopea
Subsection
Dryobalanoides
Subsection
Sphaerocarpae
Subsection
PierreaHopea clade
Dryobalanoides
clade
Outgroup
72-bp deletion
in the trnL-trnF
H
o
p
e
a
D
r
y
o
b
a
l
a
n
o
i
d
e
s
Phylogeny based on the trnL-trnF, trnT-trnL and atpB-rbcL sequences.
Upuna borneensis
H. subalata
72
96
H. bilitonensis
Tree length = 143
CI = 0.8811
RI = 0.8651
H. pubescens
21. Chloroplast genome for biotechnology
Conferring stress tolerance
n the past decade, chloroplast genetic engineering has focused
primarily on the overexpression of target genes with the potential
to enhance biotic stress tolerance, which is very important for
plant protection and yield enhancement. Yield loss due to insect
pests can be very serious in many countries. In addition to cotton
bollworm resistance conferred by hyper-expression of Bt protein
in chloroplasts [137], there are many other striking recent
examples of improved biotic stress tolerance.
22. Retrocyclin-101 and Protegrin-1 protect against Erwinia soft rot and
tobacco mosaic virus (TMV), which result in yield loss in several
cultivated crops [140]. Whitefly and aphid resistance has been
accomplished by expressing β-glucosidase [141], which releases
insecticidal sugar esters from hormone conjugates. Multiple
resistances against aphids, whiteflies, lepidopteran insects, and
bacterial and viral pathogens were achieved by expressing
the Pinellia ternata agglutinin (PTA) gene in the chloroplast genome
[142]. More than 40 transgenes have been stably integrated into and
expressed within the chloroplast genome, conferring important
agronomic traits, including insect resistance in edible crops cabbage
(Brassica oleracea) [143], soybean [144, 145], and eggplant
(Solanum melongena) [146].
23. Definition: A chloroplast
genome is a circular, double-
stranded DNA molecule located
in stroma of chloroplast.
Chloroplast genomes are highly
conserved among plant species.
There is more than one copy of
genome in each chloroplast. The
exact number varies during
development, but mesophyll
cells in young leaves contain
about 100 copies of genome.
24. Origin: All plastids studied to date contain their own
DNA, which is actually a reduced "genome" derived from
a cyanobacterial ancestor that was captured early in the
evolution of the eukaryotic cell. The genomes of the
organelles have maintained many of the features of their
prokaryote ancestors. They are circular, like prokaryotes,
and do not form complex structures like chromatin of
nuclear genome. Some of the genes are organized as
operons, with common regulatory element or promoter.
25. Function: Most of the chloroplast genome are involved in either protei
synthesis, or photosynthesis (note that most of the genes encoding
proteins participating in photosynthesis are located in nucleus). Many o
genes are in clusters, allowing expression in form of large polycistronic
mRNA transcripts, which are processed to oligo- or monocistronic
mRNAs.
27. The chloroplast genome of
maize (Zea mays) is 140,387
base pairs. It contains a pair if
inverted repeats, IRa and IRb,
which are separated by small
single-copy region (SSC of
12,536 bp) and large single-
copy region (LSC of 82,355 bp)
. Genes drawn outside the circle
are transcribed clockwise.
28. Applications in Genetic engineering: Chloroplasts are
maternally inherited. This fact is exploited for clean
gene technology . There is no danger to any gene
transfer to related weedy species through pollen. It also
ensures very large gene copy number. One of the major
additional advantage is absence of reports of gene
silencing with chloroplast transformation. These facts
combined with ability of chloroplast to correctly fold
and crosslink transformed proteins, means that there is
tremendous potential in chloroplat transformation for
very high level gene expression and synthesis of active
proteins.
29. Chloroplast genomes are typically 100–200 kbp in size, and include a set
of genes for proteins essential to photosynthesis. Other genes present in
the ancestral symbiont have been lost or relocated to the nucleus.
Chloroplasts originated in the ancestor of plants and red and green algae
by endosymbiotic acquisition of a cyanobacterium, and then spread to
many other eukaryotic lineages.
In many organisms, gene transfer from chloroplast to nucleus can still
take place, at an unexpectedly high frequency.
Many nonphotosynthetic organisms retain remnant chloroplasts, with
genomes, reflecting the fact that photosynthesis is not the only
biochemical process that takes place in the chloroplast.
In many organisms, gene transfer from chloroplast to nucleus can still
take place, at an unexpectedly high frequency.
Establishment of the chloroplast has resulted in the development of
nuclear encoded RNA binding protein families and, in land plants,‐ ‐
additional RNA polymerases that play a central role in chloroplast gene
expression.