This document summarizes work on tissue engineering of the nasal septum using a chitosan-based scaffold. Chitosan is derived from crustacean shells and has advantages like biodegradability and biocompatibility. The objective is to use a chitosan-collagen membrane seeded with human inferior turbinate cells to regenerate nasal septum tissue. Cells were cultured on the membrane and observed under a microscope to examine ciliary beat movement, which is important for nasal function. The work aims to provide an alternative to conventional nasal septum repairs that have limitations in tissue availability and prosthetic complications.

1. Tissue Engineering of Nasal Septum Using Chitosan-
Based Material
by
Arunkumar rengaraj
(22141800)

2. Introduction
• Nasal septum divides the nasal chamber into two cavities.
• Conventional repair of nasal septum involves autologous rib or septum cartilage grafts and prosthetic devices.
• Its having limitations like tissue availability, and prosthesis related infection and extrusion.
• The objective of my work is to use chitosan based scaffold for nasal septum tissue engineering.
• Chitosan, a linear polysaccharide derived from crustacean shells and from many natural sources.
• It having advantages like polycationic nature, biodegradability, biocompatibility, mucoadhesiveness.
• In addition, it enhances epithelial permeability through the opening of tight junctions between epithelial cells.

3. Materials and methods
2 % solution of chitosan chitosan + 1 M acetic acid
chitosan-collagen membrane
Tissues (Human inferior turbinates ) were treated with 0.5% pronase+1:1 mixture (DMEM) +
Ham’s nutrient F12
centrifugation
suspended in DMEM/F12
1.5-mL cell suspension was seeded on collagen coated
Morphological Examination
• The ciliary beat was observed under inverted microscope (Leica, DMI 6000, Solms, Germany) with 400 magnification.
• Imaging of cilia movement was recorded at 240 frames per second with a high-speed complementary metal oxide
semiconductor (CMOS) camera (CMC1300; VDS Vossku hler GmbH, Osnabru ck, Germany).
spread on a glass plate
Evaporated at 50C
Mix 40 μg type I collagen

4. Reference
• Dong-Won Lee, Shawna A Shirley, Richard F Lockey and Shyam Mohapatra. Thiolated chitosan nanoparticles enhance anti-
inflammatory effects of intranasally delivered theophylline. Respiratory Research 2006; 7:112.
• Tsung-Wei Huang, Yi-Ho Young, Po-Wen Cheng, Yen-Hui Chan, Tai-Horng Young. Culture of Nasal Epithelial Cells Using
Chitosan-Based Membranes. The Laryngoscope 2009; 119:2066-2070.