Platelet concentrates show promise for accelerating wound healing in regenerative dentistry. Platelets contain growth factors that can enhance the body's natural healing process. There are two main types of platelet concentrate - platelet-rich plasma (PRP) and platelet-rich fibrin (PRF). PRF has advantages over PRP as it requires no biochemical handling or additives. Potential applications of PRF in dentistry include uses in endodontics, periodontics, oral surgery, and implantology to aid bone regeneration, wound healing, and tissue engineering. While preliminary research is promising, more large-scale clinical studies are still needed to fully evaluate the effectiveness of platelet concentrates.

1. S

2. PLATELET CONCENTRATES-
Bioengineering Dentistry’s Regenerative Dreams…
AKSHAY DHANDE 4TH BDS
GOVERNAMENT DENTAL COLLEGE AND
HOSPITAL,HYDERABAD.

3. INTRODUCTION:
 Tissue regeneration is a thought provoking area in the field of dentistry.
 Platelets had been proven to be a good source of growth factors.
 Using platelet concentrates, is a way to accelerate and enhance the body’s
natural wound healing mechanisms.

4. HISTORY:
 Regenerative potential of platelets was introduced in 1970’s and its clinical
applications began in the field of medicine in 1980’s.
 Fibrin glue in Europe in 1970’s.
 Whitman et al -Platelet rich plasma in 1997
 Platelet rich fibrin was first developed in
France by Choukroun et al in 2001.
DR.CHOUKROUN

5. “The richest of resources in
nature, lie in the nature itself ”

6. UNDERSTANDING PLATELETS…
 Arise from cytoplasmic fragmentation of Megakaryocyte.
 Enter the circulation as α-nuclear cells.
 Has a life span of 7 – 10 days.
 They measure 2–3 μm in diameter.

7. MECHANISM OF WOUND HEALING :

9. CLASSIFICATION OF PLATELET CONCENTRATES :
Platelet Rich
Plasma
Platelet Rich
fibrin
P-PRP L-PRP
P-PRF L-PRF

10. CLASSIFICATION :
FIRST GENERATION
SECOND GENERATION
P-PRP Without leucocytes,low density fibrin Liquid or gel solutions
L-PRP With leucocytes , low density fibrin Liquid or gel solutions
P-PRF Without leucocytes ,high density fibrin strong gel forms,solid material
L-PRF With leucocytes ,high density fibrin strong gel forms,solid material
I-PRF Injectable form
A-PRF Advanced form
T-PRF Titanium tubes

11. PREPARATIONS OF PLATELET
CONCENTRATES

12. PREPARATION OF PRP

13. PREPARATION OF PRF
10 ml sample collection sample in test tubes centrifugation at 3000 rpm
for 10 min
after centrifugation

14. PRF PRF in sterile metal cup
PRF is placed on the grid PRF setup box PRF membranes

15. ADVANTAGES OF PRF OVER PRP
 No biochemical handling of blood.
 Simplified and cost effective process.
 No use of bovine thrombin and anticoagulants.
 Favorable healing due to low polymerization.
 More efficient cell migration and proliferation.
 Supportive effect on immune system.
 Helps in haemostasis.

16. APPLICATIONS OF Platelets RICH
FIBRIN IN DENTISTRY…

17. IN ENDODONTICS
 The use of triple antibiotic paste for canal disinfection along with PRF
strengthens the effectiveness of sterilization in carious teeth, infected dentin,
periapical lesions and necrotic pulp.
 PRF causes proliferation of pulp cells and also increases the expression of
osteoprotegerin and Alkaline phosphatase activity.
 PRF and tricalcium phosphate(TCP) bone graft have synergistic effect in
treating periapical cysts.

18.  Biological connector for neoangiogenesis and vascularization.
 Apical matrix barrier for in root end apexification.
 Apical plug in apexification.
 Pulpal floor perforation repair.
 As a scaffold for dentin pulp regeneration.

19. Periapical cyst treated with PRF
PRF in pulp regeneration periapical inflammation

20. IN PERIODONTICS
 Healing biomaterial in implant and periodontal surgical procedure.
 Osteo conductive and Osteo inductive properties.
 Good bone fill along with gain in clinical attachment was seen when PRF was
used in addition to hydroxyapatite.
 Treating intrabony defects and furcations.
 Sinus lift procedures.

21. Furcation treated with PRF PRF in root coverage procedure

22. IN ORAL AND MAXILLOFACIAL SURGERY:
Cyst treated with PRF Extraction sockets with PRF

23. Residual defect after extraction treated with bone graft and PRF
Developing alveolar ridge in anterior maxilla

24. IN IMPLANTOLOGY:
 PRP when coated on the surface of an implant releases an array of growth
factors which enhance the early wound healing providing an initial
stabilization for the implant.
 Better osseointegration capability.
 PRF as sinus augmentation adjunct
and suggested simultaneous sinus lift
implantation using PRF as a sole
grafting material.

25. CONCLUSION
 Platelet concentrates are a novel ingress of tissue engineering
to clinicians and researchers in the field of dentistry.
 Being a completely natural, physiologic, and economical source of autologous
product, it possesses beneficial effects of eliminating concerns about
immunogenic reactions and disease transmission.
 However, since knowledge on this topic is still in its preliminary stage, the
effectiveness of these platelet concentrates in regenerative procedures should
be evaluated in studies comprising of large samples and their clinical
applications in randomized control trials has to be encouraged.

26. REFERENCES
 Journal of Dental Research and Review.
 International Journal of Medicine.
 The Korean Academy of Conservative Dentistry.
 Journal of Conservative Dentistry and Endodontics.
 The Saudi Journal for Dental Research.
 Annals of Oral & Maxillofacial Surgery.

27. “Those things are better which are
perfected by nature than those which are
finished by art”
-De Nature Decorum

28. THANK YOU