Cells: Prokaryote vs
Eukaryote
Cells have evolved two
different architectures:
Prokaryote “style”
Eukaryote “style”
Prokaryote cells are
smaller and simpler
Commonly known as bacteria
10-100 microns in size
Single-celled(unicellular) or
Filamentous (strings of single cells)
These are
prokaryote
E. coli bacteria
on the head of a
steel pin.
Prokaryote cells are simply built
(example: E. coli)
capsule: slimy outer
coating
cell wall: tougher middle
layer
cell membrane: delicate
inner skin
Prokaryote cells are simply built
(example: E. coli)
cytoplasm: inner liquid filling
DNA in one big loop
pilli: for sticking to things
flagella: for swimming
ribosomes: for building
proteins
Prokaryote lifestyle
unicellular: all
alone
colony: forms a
film
filamentous:
forms a chain of
cells
Prokaryote Feeding
Photosynthetic: energy from sunlight
Disease-causing: feed on living things
Decomposers: feed on dead things
Eukaryotes are bigger and more
complicated
Have organelles
Have chromosomes
can be multicellular
include animal and plant cells
Organelles are
membrane-bound cell
parts
Mini “organs” that have
unique structures and
functions
Located in cytoplasm
Cell Structures
Cell membrane
delicate lipid
and protein
skin around
cytoplasm
found in all
cells
Nucleus
a membrane-bound
sac evolved to store
the cell’s
chromosomes(DNA
)
has pores: holes
Nucleolus
inside nucleus
location of
ribosome
factory
made or
RNA
mitochondrion
makes the cell’s
energy
the more energy
the cell needs,
the more
mitochondria it
has
Ribosomes
build proteins
from amino acids
in cytoplasm
may be freefloating, or
may be attached
to ER
made of RNA
Endoplasmic
reticulum
may be smooth:
builds lipids and
carbohydrates
may be rough:
stores proteins
made by
attached
ribosomes
Golgi Complex
takes in sacs
of raw
material from
ER
sends out sacs
containing
finished cell
products
Lysosomes
sacs filled with
digestive enzymes
digest worn out
cell parts
digest food
absorbed by cell
Centrioles
pair of bundled
tubes
organize cell
division
Cytoskeleton
made of
microtubules
found throughout
cytoplasm
gives shape to cell
& moves organelles
around inside.
Structures found in plant cells
Cell wall
very strong
made of
cellulose
protects cell
from rupturing
glued to other
cells next door
Vacuole
huge waterfilled sac
keeps cell
pressurized
stores starch
Chloroplasts
filled with
chlorophyll
turn solar
energy into
food energy
How are plant and animal cells different?
Structure
cell membrane
nucleus
nucleolus
ribosomes
ER
Golgi
centrioles
cell wall
mitochondria
cholorplasts
One big vacuole
cytoskeleton

Animal cells
Yes
Yes
yes
yes
yes
yes
yes
no
yes
no
no
yes

Plant cells
yes
yes
yes
yes
yes
yes
no
yes
yes
yes
yes
Yes
Eukaryote cells can be
multicellular
The whole cell can be specialized for one
job
cells can work together as tissues
Tissues can work together as organs
Advantages of each kind of
cell architecture
Prokaryotes

Eukaryotes

simple and easy to grow

can specialize

fast reproduction

multicellularity

all the same

can build large bodies
Examples of specialized
euk. cells
liver cell:
specialized to
detoxify blood
and store
glucose as
glycogen.
sperm cell:
specialized to
deliver DNA
to egg cell
Mesophyll cell
specialized
to capture
as much
light as
possible
inside a
leaf
How do animal cells
move?
Some can crawl with pseudopods
Some can swim with a flagellum
Some can swim very fast with cilia
Pseudopods
means “fake feet”
extensions of cell
membrane
example: ameoba
Flagellum/flagella
large whiplike tail
pushes or pulls cell
through water
can be single, or a
pair
Cilia
fine, hairlike
extensions
attached to cell
membrane
beat in unison
How did organelles evolve?
many scientists theorize that
eukaryotes evolved from
prokaryote ancestors.
in 1981, Lynn Margulis
popularized the
“endosymbiont theory.”
Endosymbiont theory:
a prokaryote ancestor
“eats” a smaller
prokaryote
the smaller prokaryote
evolves a way to avoid
being digested, and lives
inside its new “host” cell
kind of like a pet.
Endo = inside
Symbiont = friend
the small prokaryotes that can do
photosynthesis evolve into chloroplasts,
and “pay” their host with glucose.
The smaller prokaryotes that can do
aerobic respiration evolve into
mitochondria, and convert the glucose into
energy the cell can use.
Both the host and the symbiont benefit
from the relationship
Chlorella are
tiny green cells
that live inside
some amoeba...
endosymbiosis
may still be
evolving today!
Eukaryotic and
Prokaryotic Cells
AHMP 5406
Objectives:
1.

Discuss the key differences between prokaryotic and eukaryotic cellular
components

2.

Describe the basic structure of mammalian eukaryotic cells

3.

Discuss the different nutritional classes

4.

Explain the endosymbiotic theory and development of organelles in the
Euk. cell

5.

Compare and contrast the genome organization of prokaryotes versus
eukaryotes

6.

Compare the different ways new genes are generated from existing genes

7.

Explain why the human genome is complex
Objectives:
1.

Discuss the key differences between prokaryotic and eukaryotic cellular
components

2.

Describe the basic structure of mammalian eukaryotic cells

3.

Discuss the different nutritional classes

4.

Explain the endosymbiotic theory and development of organelles in the
Euk. cell

5.

Compare and contrast the genome organization of prokaryotes versus
eukaryotes

6.

Compare the different ways new genes are generated from existing genes

7.

Explain why the human genome is complex
1

Features All Cells Share
Hereditary info stored in DNA
Proteins are used as catalysts
DNA replication occurs through templated polymerization
Hereditary info transcribed into RNA
RNA translated into protein
Later we will discuss these things in greater detail
From DNA to Protein
(Why is this eukaryotic?)
1

Prokaryotic cells:
No membrane bound organelles
Simple genome organization
Genes usually do not have introns
Between 500 and 4000 genes
M. genitalium has 477 genes
Great degree of diversity
Unicellular
1

Eukaryotic cells
All organelles are membrane-bound
Complex genome organization
Large genome size
Between 6,000 and 30,000 genes
Genes have introns
Where is the genetic diversity?

Large amount of regulatory DNA
To control gene expression

High degree of genetic redundancy
Unicellular or multicellular
1
Objectives:
1.

Discuss the key differences between prokaryotic and eukaryotic
cellular components

2.

Describe the basic structure of mammalian eukaryotic cells

3.

Discuss the different nutritional classes

4.

Explain the endosymbiotic theory and development of organelles
in the Euk. cell

5.

Compare and contrast the genome organization of prokaryotes
versus eukaryotes

6.

Compare the different ways new genes are generated from
existing genes

7.

Explain why the human genome is complex
1 and 2

General eukaryotic cell
Objectives:
1.

Discuss the key differences between prokaryotic and eukaryotic cellular
components

2.

Describe the basic structure of mammalian eukaryotic cells

3.

Discuss the different nutritional classes

4.

Explain the endosymbiotic theory and development of organelles in the
Euk. cell

5.

Compare and contrast the genome organization of prokaryotes versus
eukaryotes

6.

Compare the different ways new genes are generated from existing genes

7.

Explain why the human genome is complex
3

Cells can be powered by different
energy sources
I eat other living things!
Organotrophic
Animals
Protists etc.

I need a tan?
Phototrohpic
Bacteria
Plants
Algae

I eat dirt?
Lithotropic
Arcaebacteria
Objectives:
1.

Discuss the key differences between prokaryotic and eukaryotic cellular
components

2.

Describe the basic structure of mammalian eukaryotic cells

3.

Discuss the different nutritional classes

4.

Explain the endosymbiotic theory and development of organelles in the
Euk. cell

5.

Compare and contrast the genome organization of prokaryotes versus
eukaryotes

6.

Compare the different ways new genes are generated from existing genes

7.

Explain why the human genome is complex
4

Eukaryotic cell may have
been predators
The primordial eukaryotic cell may have survived by
eating other cells
This would require:
A large cell
A flexible membrane
An elaborate cytoskeleton
Protected DNA (in an organelle, which one?)
4

Dinidium is a cilliated protozoan

Lunch!

He looks hungry
4
4
4
Objectives:
1.

Discuss the key differences between prokaryotic and eukaryotic
cellular components

2.

Describe the basic structure of mammalian eukaryotic cells

3.

Discuss the different nutritional classes

4.

Explain the endosymbiotic theory and development of organelles
in the Euk. cell

5.

Compare and contrast the genome organization of prokaryotes
versus eukaryotes

6.

Compare the different ways new genes are generated from
existing genes

7.

Explain why the human genome is complex
5

Genome organization in
Prok. and Euk.
Prokaryotes
Circular DNA
mtDNA very similar

Eukaryotes
Linear DNA
nuclear DNA
5

Prokaryotic DNA
5

Eukaryotic DNA
DNA packaged in a chromosome

Linear DNA

Associated proteins

More details later
Objectives:
1.

Discuss the key differences between prokaryotic and eukaryotic
cellular components

2.

Describe the basic structure of mammalian eukaryotic cells

3.

Discuss the different nutritional classes

4.

Explain the endosymbiotic theory and development of organelles
in the Euk. cell

5.

Compare and contrast the genome organization of prokaryotes
versus eukaryotes

6.

Compare the different ways new genes are generated from
existing genes

7.

Explain why the human genome is complex
6
Ways to generate new genes from preexisting genes
Intragenic mutation
Simply a mutation in the sequence

Gene duplication
Whole gene is just recopied into different part of the genome

DNA segment shuffling
Similar genes may exchange segments

Horizontal transfer
Cell to cell transfer of genetic material
e.g. Plasmids
ancient mitochondrial genes
Viruses
Sex
6

Gene Families
Created by generation of different genes from a
precursor
Speciation results in orthologous genes
An organismal phylogeny is produced
Gene duplication can result in paralogous genes
A gene phylogeny is produced
Objectives:
1.

Discuss the key differences between prokaryotic and eukaryotic
cellular components

2.

Describe the basic structure of mammalian eukaryotic cells

3.

Discuss the different nutritional classes

4.

Explain the endosymbiotic theory and development of organelles
in the Euk. cell

5.

Compare and contrast the genome organization of prokaryotes
versus eukaryotes

6.

Compare the different ways new genes are generated from
existing genes

7.

Explain why the human genome is complex
7

The human genome is complex:

Partly because of duplications
7

Regulatory
DNA is
everywhere
in eukaryotic
genomes
7

mRNA Structure
7

Comparative Genome Sizes

Cells 2

  • 1.
  • 2.
    Cells have evolvedtwo different architectures: Prokaryote “style” Eukaryote “style”
  • 3.
    Prokaryote cells are smallerand simpler Commonly known as bacteria 10-100 microns in size Single-celled(unicellular) or Filamentous (strings of single cells)
  • 4.
    These are prokaryote E. colibacteria on the head of a steel pin.
  • 5.
    Prokaryote cells aresimply built (example: E. coli) capsule: slimy outer coating cell wall: tougher middle layer cell membrane: delicate inner skin
  • 6.
    Prokaryote cells aresimply built (example: E. coli) cytoplasm: inner liquid filling DNA in one big loop pilli: for sticking to things flagella: for swimming ribosomes: for building proteins
  • 7.
    Prokaryote lifestyle unicellular: all alone colony:forms a film filamentous: forms a chain of cells
  • 8.
    Prokaryote Feeding Photosynthetic: energyfrom sunlight Disease-causing: feed on living things Decomposers: feed on dead things
  • 9.
    Eukaryotes are biggerand more complicated Have organelles Have chromosomes can be multicellular include animal and plant cells
  • 10.
    Organelles are membrane-bound cell parts Mini“organs” that have unique structures and functions Located in cytoplasm
  • 11.
    Cell Structures Cell membrane delicatelipid and protein skin around cytoplasm found in all cells
  • 12.
    Nucleus a membrane-bound sac evolvedto store the cell’s chromosomes(DNA ) has pores: holes
  • 13.
  • 14.
    mitochondrion makes the cell’s energy themore energy the cell needs, the more mitochondria it has
  • 15.
    Ribosomes build proteins from aminoacids in cytoplasm may be freefloating, or may be attached to ER made of RNA
  • 16.
    Endoplasmic reticulum may be smooth: buildslipids and carbohydrates may be rough: stores proteins made by attached ribosomes
  • 17.
    Golgi Complex takes insacs of raw material from ER sends out sacs containing finished cell products
  • 18.
    Lysosomes sacs filled with digestiveenzymes digest worn out cell parts digest food absorbed by cell
  • 19.
  • 20.
    Cytoskeleton made of microtubules found throughout cytoplasm givesshape to cell & moves organelles around inside.
  • 21.
    Structures found inplant cells Cell wall very strong made of cellulose protects cell from rupturing glued to other cells next door
  • 22.
    Vacuole huge waterfilled sac keepscell pressurized stores starch
  • 23.
  • 24.
    How are plantand animal cells different?
  • 25.
    Structure cell membrane nucleus nucleolus ribosomes ER Golgi centrioles cell wall mitochondria cholorplasts Onebig vacuole cytoskeleton Animal cells Yes Yes yes yes yes yes yes no yes no no yes Plant cells yes yes yes yes yes yes no yes yes yes yes Yes
  • 26.
    Eukaryote cells canbe multicellular The whole cell can be specialized for one job cells can work together as tissues Tissues can work together as organs
  • 27.
    Advantages of eachkind of cell architecture Prokaryotes Eukaryotes simple and easy to grow can specialize fast reproduction multicellularity all the same can build large bodies
  • 28.
    Examples of specialized euk.cells liver cell: specialized to detoxify blood and store glucose as glycogen.
  • 29.
  • 30.
    Mesophyll cell specialized to capture asmuch light as possible inside a leaf
  • 31.
    How do animalcells move? Some can crawl with pseudopods Some can swim with a flagellum Some can swim very fast with cilia
  • 32.
    Pseudopods means “fake feet” extensionsof cell membrane example: ameoba
  • 33.
    Flagellum/flagella large whiplike tail pushesor pulls cell through water can be single, or a pair
  • 34.
    Cilia fine, hairlike extensions attached tocell membrane beat in unison
  • 35.
    How did organellesevolve? many scientists theorize that eukaryotes evolved from prokaryote ancestors. in 1981, Lynn Margulis popularized the “endosymbiont theory.”
  • 36.
    Endosymbiont theory: a prokaryoteancestor “eats” a smaller prokaryote the smaller prokaryote evolves a way to avoid being digested, and lives inside its new “host” cell kind of like a pet.
  • 37.
  • 38.
    the small prokaryotesthat can do photosynthesis evolve into chloroplasts, and “pay” their host with glucose. The smaller prokaryotes that can do aerobic respiration evolve into mitochondria, and convert the glucose into energy the cell can use. Both the host and the symbiont benefit from the relationship
  • 39.
    Chlorella are tiny greencells that live inside some amoeba... endosymbiosis may still be evolving today!
  • 40.
  • 41.
    Objectives: 1. Discuss the keydifferences between prokaryotic and eukaryotic cellular components 2. Describe the basic structure of mammalian eukaryotic cells 3. Discuss the different nutritional classes 4. Explain the endosymbiotic theory and development of organelles in the Euk. cell 5. Compare and contrast the genome organization of prokaryotes versus eukaryotes 6. Compare the different ways new genes are generated from existing genes 7. Explain why the human genome is complex
  • 42.
    Objectives: 1. Discuss the keydifferences between prokaryotic and eukaryotic cellular components 2. Describe the basic structure of mammalian eukaryotic cells 3. Discuss the different nutritional classes 4. Explain the endosymbiotic theory and development of organelles in the Euk. cell 5. Compare and contrast the genome organization of prokaryotes versus eukaryotes 6. Compare the different ways new genes are generated from existing genes 7. Explain why the human genome is complex
  • 43.
    1 Features All CellsShare Hereditary info stored in DNA Proteins are used as catalysts DNA replication occurs through templated polymerization Hereditary info transcribed into RNA RNA translated into protein Later we will discuss these things in greater detail
  • 44.
    From DNA toProtein (Why is this eukaryotic?)
  • 45.
    1 Prokaryotic cells: No membranebound organelles Simple genome organization Genes usually do not have introns Between 500 and 4000 genes M. genitalium has 477 genes Great degree of diversity Unicellular
  • 46.
    1 Eukaryotic cells All organellesare membrane-bound Complex genome organization Large genome size Between 6,000 and 30,000 genes Genes have introns Where is the genetic diversity? Large amount of regulatory DNA To control gene expression High degree of genetic redundancy Unicellular or multicellular
  • 47.
  • 48.
    Objectives: 1. Discuss the keydifferences between prokaryotic and eukaryotic cellular components 2. Describe the basic structure of mammalian eukaryotic cells 3. Discuss the different nutritional classes 4. Explain the endosymbiotic theory and development of organelles in the Euk. cell 5. Compare and contrast the genome organization of prokaryotes versus eukaryotes 6. Compare the different ways new genes are generated from existing genes 7. Explain why the human genome is complex
  • 49.
    1 and 2 Generaleukaryotic cell
  • 50.
    Objectives: 1. Discuss the keydifferences between prokaryotic and eukaryotic cellular components 2. Describe the basic structure of mammalian eukaryotic cells 3. Discuss the different nutritional classes 4. Explain the endosymbiotic theory and development of organelles in the Euk. cell 5. Compare and contrast the genome organization of prokaryotes versus eukaryotes 6. Compare the different ways new genes are generated from existing genes 7. Explain why the human genome is complex
  • 51.
    3 Cells can bepowered by different energy sources I eat other living things! Organotrophic Animals Protists etc. I need a tan? Phototrohpic Bacteria Plants Algae I eat dirt? Lithotropic Arcaebacteria
  • 52.
    Objectives: 1. Discuss the keydifferences between prokaryotic and eukaryotic cellular components 2. Describe the basic structure of mammalian eukaryotic cells 3. Discuss the different nutritional classes 4. Explain the endosymbiotic theory and development of organelles in the Euk. cell 5. Compare and contrast the genome organization of prokaryotes versus eukaryotes 6. Compare the different ways new genes are generated from existing genes 7. Explain why the human genome is complex
  • 53.
    4 Eukaryotic cell mayhave been predators The primordial eukaryotic cell may have survived by eating other cells This would require: A large cell A flexible membrane An elaborate cytoskeleton Protected DNA (in an organelle, which one?)
  • 54.
    4 Dinidium is acilliated protozoan Lunch! He looks hungry
  • 55.
  • 56.
  • 57.
  • 58.
    Objectives: 1. Discuss the keydifferences between prokaryotic and eukaryotic cellular components 2. Describe the basic structure of mammalian eukaryotic cells 3. Discuss the different nutritional classes 4. Explain the endosymbiotic theory and development of organelles in the Euk. cell 5. Compare and contrast the genome organization of prokaryotes versus eukaryotes 6. Compare the different ways new genes are generated from existing genes 7. Explain why the human genome is complex
  • 59.
    5 Genome organization in Prok.and Euk. Prokaryotes Circular DNA mtDNA very similar Eukaryotes Linear DNA nuclear DNA
  • 60.
  • 61.
    5 Eukaryotic DNA DNA packagedin a chromosome Linear DNA Associated proteins More details later
  • 62.
    Objectives: 1. Discuss the keydifferences between prokaryotic and eukaryotic cellular components 2. Describe the basic structure of mammalian eukaryotic cells 3. Discuss the different nutritional classes 4. Explain the endosymbiotic theory and development of organelles in the Euk. cell 5. Compare and contrast the genome organization of prokaryotes versus eukaryotes 6. Compare the different ways new genes are generated from existing genes 7. Explain why the human genome is complex
  • 63.
    6 Ways to generatenew genes from preexisting genes Intragenic mutation Simply a mutation in the sequence Gene duplication Whole gene is just recopied into different part of the genome DNA segment shuffling Similar genes may exchange segments Horizontal transfer Cell to cell transfer of genetic material e.g. Plasmids ancient mitochondrial genes Viruses Sex
  • 64.
    6 Gene Families Created bygeneration of different genes from a precursor Speciation results in orthologous genes An organismal phylogeny is produced Gene duplication can result in paralogous genes A gene phylogeny is produced
  • 65.
    Objectives: 1. Discuss the keydifferences between prokaryotic and eukaryotic cellular components 2. Describe the basic structure of mammalian eukaryotic cells 3. Discuss the different nutritional classes 4. Explain the endosymbiotic theory and development of organelles in the Euk. cell 5. Compare and contrast the genome organization of prokaryotes versus eukaryotes 6. Compare the different ways new genes are generated from existing genes 7. Explain why the human genome is complex
  • 66.
    7 The human genomeis complex: Partly because of duplications
  • 67.
  • 68.
  • 69.