Presentation on the application and impact of next generation sequencing in studies of cave animals and other subterranean species. Held at the 23rd International Conference on Subterranean Biology in the Department of Biology at the University of Arkansas, 13-17 June 2016, Fayetteville, Arkansas.
Variation in taxonomic and systematic charactersNoor Zada
There are three main types of variation: geographic, sexual, and individual. Geographic variation occurs over space and can lead to clinal variation correlated with environmental factors. Sexual variation involves differences between males and females, often involving primary and secondary sexual characteristics. Individual variation occurs within the lifetime of an organism and can be due to factors like age, environment, parasites, and genetics. Genetic variation includes sex-associated traits and non-sex associated continuous or discontinuous variation within populations.
1. Intrapopulation variations can be genetic or non-genetic. Non-genetic variations include individual, social, ecological, and traumatic variations.
2. Individual variations over time include age variations, seasonal variations within an individual's lifetime, and seasonal variations across generations.
3. Social variations occur in social insects where there are distinct castes or groups within a colony serving different functions.
4. Ecological variations arise from differences in habitat, climate conditions, host organisms, population density, growth patterns, and neurogenic responses to the environment.
Hormones play an important role in human development. The main hormones involved are growth hormone, thyroid hormone, sex hormones like testosterone and estrogen, and pituitary gonadotropic hormones. Growth hormone stimulates growth of cells and tissues. Thyroid hormone is necessary for normal growth and brain development. Testosterone promotes male sexual development and the growth spurt during puberty. Estrogens stimulate female sexual development and changes at puberty like breast growth. The pituitary also secretes follicle-stimulating hormone and luteinizing hormone which regulate the development and function of the ovaries and testes.
The history of taxonomy is described in four periods by Mayr. The first period focused on local fauna studies by ancient Greek scholars like Aristotle. The second period was marked by the acceptance of evolution, influenced by Darwin's ideas. The third period saw the rise of population systematics in the 1930s, where Mayr defined species as interbreeding natural populations. Current taxonomy in the fourth period utilizes a wide range of data beyond just morphology, including genetics, biochemistry, and behavior.
The document discusses microbial taxonomy and classification systems. It describes the levels of classification from kingdom to species. A species is defined as a collection of microbial strains that share many properties and differ from other groups. Useful properties for microbial classification include morphology, biochemical characteristics, nucleic acid sequencing, and phylogeny based on rRNA sequences. The three domains of life are described as Archaea, Bacteria, and Eucarya. Notable bacterial phyla like Proteobacteria, Firmicutes, and Actinobacteria are also summarized.
This document discusses the evolution of the theory of biological nomenclature. It begins by defining nomenclature and explaining how Carl Linnaeus established the system of binomial nomenclature in 1758. This system uses a two-part Latin name for each organism, with the first part indicating the genus and the second indicating the species. The document outlines the basic rules of binomial nomenclature and three essential requisites: uniqueness, universality, and stability. It also discusses how the International Code of Zoological Nomenclature codifies and regulates the rules, and how it has evolved over the last 150 years through various international bodies and congresses.
Natural selection leads to the evolution of organisms over time through differential survival and reproduction based on heritable traits. There are three main types of selection: directional selection favors one trait extreme, stabilizing selection favors intermediate traits, and disruptive selection favors opposite trait extremes. Examples of natural selection in action include the evolution of melanism in moths due to pollution and the evolution of myxoma virus resistance in rabbits in Australia. Laboratory studies also demonstrate the effects of selection, such as increased growth in selectively bred salmon. Natural selection can result in radiation, convergence, regression, extinction, and coevolution between interacting species.
Macroevolution refers to large evolutionary changes that occur at or above the species level, in contrast to microevolution which involves smaller changes within a species. Adaptive radiation is the evolution of many descendant species from a single ancestor, with each species adapted to a different ecological niche. A classic example is Darwin's finches, which evolved from a common ancestor into 14 species with varying beak sizes and shapes adapted to different food sources. Orthogenesis proposed that evolutionary changes occur in a straight line, while allometry describes how body part size relates to overall body size and its evolution.
Variation in taxonomic and systematic charactersNoor Zada
There are three main types of variation: geographic, sexual, and individual. Geographic variation occurs over space and can lead to clinal variation correlated with environmental factors. Sexual variation involves differences between males and females, often involving primary and secondary sexual characteristics. Individual variation occurs within the lifetime of an organism and can be due to factors like age, environment, parasites, and genetics. Genetic variation includes sex-associated traits and non-sex associated continuous or discontinuous variation within populations.
1. Intrapopulation variations can be genetic or non-genetic. Non-genetic variations include individual, social, ecological, and traumatic variations.
2. Individual variations over time include age variations, seasonal variations within an individual's lifetime, and seasonal variations across generations.
3. Social variations occur in social insects where there are distinct castes or groups within a colony serving different functions.
4. Ecological variations arise from differences in habitat, climate conditions, host organisms, population density, growth patterns, and neurogenic responses to the environment.
Hormones play an important role in human development. The main hormones involved are growth hormone, thyroid hormone, sex hormones like testosterone and estrogen, and pituitary gonadotropic hormones. Growth hormone stimulates growth of cells and tissues. Thyroid hormone is necessary for normal growth and brain development. Testosterone promotes male sexual development and the growth spurt during puberty. Estrogens stimulate female sexual development and changes at puberty like breast growth. The pituitary also secretes follicle-stimulating hormone and luteinizing hormone which regulate the development and function of the ovaries and testes.
The history of taxonomy is described in four periods by Mayr. The first period focused on local fauna studies by ancient Greek scholars like Aristotle. The second period was marked by the acceptance of evolution, influenced by Darwin's ideas. The third period saw the rise of population systematics in the 1930s, where Mayr defined species as interbreeding natural populations. Current taxonomy in the fourth period utilizes a wide range of data beyond just morphology, including genetics, biochemistry, and behavior.
The document discusses microbial taxonomy and classification systems. It describes the levels of classification from kingdom to species. A species is defined as a collection of microbial strains that share many properties and differ from other groups. Useful properties for microbial classification include morphology, biochemical characteristics, nucleic acid sequencing, and phylogeny based on rRNA sequences. The three domains of life are described as Archaea, Bacteria, and Eucarya. Notable bacterial phyla like Proteobacteria, Firmicutes, and Actinobacteria are also summarized.
This document discusses the evolution of the theory of biological nomenclature. It begins by defining nomenclature and explaining how Carl Linnaeus established the system of binomial nomenclature in 1758. This system uses a two-part Latin name for each organism, with the first part indicating the genus and the second indicating the species. The document outlines the basic rules of binomial nomenclature and three essential requisites: uniqueness, universality, and stability. It also discusses how the International Code of Zoological Nomenclature codifies and regulates the rules, and how it has evolved over the last 150 years through various international bodies and congresses.
Natural selection leads to the evolution of organisms over time through differential survival and reproduction based on heritable traits. There are three main types of selection: directional selection favors one trait extreme, stabilizing selection favors intermediate traits, and disruptive selection favors opposite trait extremes. Examples of natural selection in action include the evolution of melanism in moths due to pollution and the evolution of myxoma virus resistance in rabbits in Australia. Laboratory studies also demonstrate the effects of selection, such as increased growth in selectively bred salmon. Natural selection can result in radiation, convergence, regression, extinction, and coevolution between interacting species.
Macroevolution refers to large evolutionary changes that occur at or above the species level, in contrast to microevolution which involves smaller changes within a species. Adaptive radiation is the evolution of many descendant species from a single ancestor, with each species adapted to a different ecological niche. A classic example is Darwin's finches, which evolved from a common ancestor into 14 species with varying beak sizes and shapes adapted to different food sources. Orthogenesis proposed that evolutionary changes occur in a straight line, while allometry describes how body part size relates to overall body size and its evolution.
The evolutionary history of camels began around 50 million years ago with their small, rabbit-sized ancestor called Protylopus in North America. Protylopus had adaptations like shorter front limbs that allowed it to browse for leaves. Changing climates led to species like Poebrotherium that were larger with tooth and leg adaptations for grasslands. Procamelus emerged during cooling periods and migrated. Paracamelus developed in the Pliocene with modern features. Camelus emerged in the Pleistocene as one-humped dromedaries in deserts. Modern camels have adaptations like humps and eyelashes for harsh desert environments.
Biological collections preserve plant and animal specimens through various methods. Dry collections involve preserving specimens without liquid through rigidity or highlighting distinguishing features. Wet collections submerge specimens in liquid preservatives to maintain body form and soft tissues. Low-temperature collections maintain specimens' viability for analysis by storing at cold temperatures. Microscopy collections prepare specimens for examination under microscopes. Proper collection, preservation, cataloging and storage help museums maintain valuable reference materials.
TO FOLLOW THESE SLIDES you will learn about the adaptive radiations involve in evolution .
yo will learn about the parallel adaptations and its types
speciation role in the evolution
factors
key innvations
to imrove the article involving examples
Founder events
Adaptive plasticity
process of adaptive radiation
Factors promote adaptive radiations
Factors underlying adaptive radiations
defined by 0.S OSBORN
ecological space
geological
climatological
Islands
examplrs: 1.Darwin Finches 2.Cichlid fish genome -adaptive evolution, Stanford scientists
3.Anolis Lizards
Factors promote adaptive radiations
1.Generally speaking, adaptive radiations occur when new, unoccupied ecological niches become accessible to a founder population.
This can happen after a mass extinction during which the previous occupiers of those niches died out.
t can also happen when a colonizing species arrives at an island. (For instance the ancestor of the honeycreepers in Hawaii, or of Darwin's "finches" in the Galapagos)
Honey creeper
Change feeding habitat
At least 56 species of Hawaiian honeycreepers known to have existed, although all but 18 of them are now extinct.
Lack of competition. When a species enters an adaptive zone, it is poorly equipped to compete with species that have become adapted to the same niche.
For example, mudskippers are fish that are making a living on land, but they are marine fish and they don't have to compete against frogs and salamanders, which are restricted to fresh water. That is why we don't see freshwater mudskippers.
process of adaptive radiation
Ecological Release Colonization of species.
Taxon cycle
Habitat varying as population expand- species dispersal.
Adaptive plasticity Phenotypic plasticity(behavior change)
Property of an individual or genotype that may be adaptive, maladaptive or neutral with regard to an individual's fitness.
The particular way an individual's (or genotype's) phenotype varies across environments can be described as a reaction norm (Single genotype-phenotypic expression)
Speciation in adaptive radiation Founder events
This document discusses nitrogenous waste and endogenous chemicals in fish tissues. It explains that nitrogenous wastes are synthesized from excess amino acids and must be excreted. The three main types of nitrogenous waste used by vertebrates are ammonia, urea, and uric acid. Endogenous chemicals in fish tissues include fatty acids and eicosanoids. Fatty acids are precursors for eicosanoids, which have many physiological functions. Eicosanoids include prostaglandins, thromboxanes, and leukotrienes.
ORIGIN OF CHORDATES
Animal kingdom is basically divided into two sub kingdoms:
Non-chordata- including animals without notochord.
Chordata- This comprising animals having notochord or chorda dorsalis.
Chordates were evolved sometime 500 million years ago during Cambrian period (invertebrates were also began to evolve in this period) .
Chamberlain (1900) pointed out that all modern chordates possess glomerular kidneys that are designed to remove excess water from body.
It is believed that Chordates have originated from invertebrates.
It is difficult to determine from which invertebrate group the chordates were developed.
Chordate ancestors were soft bodied animals. Hence they were not preserved as Fossils.
However, early fossils of chordates have all been recovered from marine sediments and even modern protochordates are all marine forms.
Also glomerular kidneys are also found in some marine forms such as myxinoids and sharks. That makes the marine origin of chordates more believable.
Chordates evolved from some deuterostome ancestor (echinoderms, hemichordates, pogonophorans etc.) as they have similarities in embryonic development, type of coelom and larval stages.
Many theories infers origin of chordates, hemichordates and echinoderms from a common ancestor.
This document discusses taxonomic characters which are attributes used to classify organisms based on similarities and differences. It covers the importance of characters in classification, their characteristics, types including morphological, physiological, behavioral, ecological and geographic. It also discusses the role of characters in modern taxonomy, character weighting, and inadmissible characters that are excluded from classification like meaningless, logically correlated, partially correlated and invariant characters.
This document discusses different types of biological variations that can occur in animals, including genetic, non-genetic, individual, social, ecological, traumatic, age-related, seasonal, and polyphenic variations. It provides examples of variations in appearance between juvenile and adult stages, between seasons, and in response to environmental conditions. It also summarizes variations that can occur between sexes, castes in social insects, populations in different habitats, in response to atypical environmental conditions, and those that involve allometric growth or neurogenic color changes.
The document discusses neutral theory of molecular evolution, which holds that most genetic changes are due to neutral mutations that do not affect organismal fitness. It proposes that neutral mutations accumulate over time at a constant rate, allowing relative divergence times to be estimated. The theory aims to explain high genetic variation and presence of neutral substitutions between species. Several lines of evidence are presented, including comparative rates of evolution between functionally important and unimportant genes and gene regions.
Taxonomic collection and identificationAftab Badshah
Biological collections are valuable for preserving extinct species and rare specimens. They allow researchers to verify original data and study specimens that are otherwise inaccessible. Effective collections involve sampling populations across species' ranges and variations, including larvae and parasites. Specimens are collected using various methods and preserved appropriately through methods like alcohol, stuffing, or formalin depending on the taxon. Proper long-term labeling with data like location, date, life stage, and collector is critical for research.
Zoological nomenclature establishes scientific names for animal taxa according to a set of international rules to ensure names are unique, universal, and stable, with each taxon having a designated type specimen to serve as the objective standard for applying its name. The principle of priority dictates that the oldest available name for a taxon is the valid name, while the principle of the first reviser resolves situations where two names have the same date. Names apply to both living and extinct animals according to these principles and rules.
Evolutionary biologists use phylogenetic trees and cladistics to study evolutionary relationships between organisms and construct classifications. Cladistics involves analyzing shared characteristics to hypothesize how groups of organisms evolved from common ancestors over time. A key assumption is that all organisms are related through descent from a shared ancestor. Cladograms graphically represent evolutionary relationships, with shared derived characteristics defining monophyletic clades. Parsimony is used to select the simplest phylogenetic tree that is best supported by evidence.
Orthogenesis is the theory that organisms evolve in a definite direction due to some internal mechanism, rejecting natural selection. Allometry describes the relationship between an organism's size and its body parts, such as brain size increasing with body size. Adaptive radiations occur when environmental changes open new niches, causing rapid speciation and phenotypic adaptation in a relatively short time, as seen with Hawaiian honeycreepers adapting to different island environments.
This document discusses evolutionary theories around sexual selection and relationships. It explains that sexual selection occurs through intrasexual competition between males to attract females, and intersexual selection where females choose their mates. Research by Buss (1989) found cross-cultural differences in mate preferences, with females prioritizing resources and males prioritizing youth and attractiveness. Studies also show females' preferences change across their menstrual cycle and they favor more masculine traits when fertile. However, critics argue these theories are reductionist and deterministic by not accounting for free will or environmental influences on relationships.
1. Microevolution leads to genetic differences between populations over time, which can result in new species through macroevolution if enough differences accumulate.
2. A polytypic species consists of multiple geographically isolated populations that have undergone microevolution, like the different tiger subspecies.
3. Recognizing polytypic species simplifies classification by reducing many similar local populations to subspecies rather than considering each a unique species. However, delineating subspecies from one another or related species remains challenging.
To determine the variation and the limitation between species, many concepts have been proposed.
When a taxonomist study a particular taxa, he/she must adopted a species concept and provide a species limitation to define this taxa.
Plant kingdom as other living kingdoms has a hierarchy structure ends mostly with species rank.
Species are one of the basic units to compare in almost all fields of biology.
A species is defined as the largest group of organisms in which two individuals are capable of reproducing fertile offspring, typically using sexual reproduction.
Definition of a species as a group of interbreeding individuals cannot be easily applied to organisms that reproduce only or mainly asexually.
If two lineages of oak look quite different, but occasionally form hybrids with each other, should we count them as different species?
Idea of a species is something that we humans invented for our own convenience.
‘‘No matter what variations occur in the individuals or the species, if they spring from the seed of one and the same plant, they are accidental variations and not such as distinguish a species permanently; one species never springs from the seed of another nor vice versa” - JOHN RAY.
Used a sexual system ‘‘natural system” for defining species - LINNAEUS.
‘‘A species is a collection of all the individuals which resemble each other more than they resemble anything else, which can by natural fecundation produce fertile individuals, and which reproduce themselves by generation, in such a manner that we may from analogy suppose them all to have sprung from one single individual” - DE CANDOLLE.
This document provides a history of taxonomy from ancient times through the modern era. It discusses how early ancient Chinese, Egyptian, Greek, and Roman scholars began classifying and organizing plants and animals. Key early taxonomists mentioned include Aristotle, Theophrastus, Dioscorides, and Pliny. It then covers the contributions of later taxonomists like Caesalpino, the Bauhin brothers, John Ray, and Joseph Pitton de Tournefort. The era of Carl Linnaeus is described as revolutionizing taxonomy through his introduction of binomial nomenclature. The document outlines how Linnaeus helped transform botany and zoology into scientific disciplines. It concludes with a discussion of developments like cl
The document discusses several trends and rates of evolution, including Dollo's law of irreversibility, Cope's law of body size increase over generations, and Gause's exclusion principle of ecological niche overlap. It also covers Bergman's rule relating body size and temperature, techniques for examining evolutionary rates like DNA analysis, and the hypotheses of gradual vs punctuated change to explain rates of evolution. Resources on these topics are listed at the end.
Physiology of Respiration in InvertebratesPRANJAL SHARMA
In physiology, respiration is the movement of oxygen from the outside environment to the cells within tissues, and the removal of carbon dioxide in the opposite direction. In these slides you will get to know about Physiology of Respiration in Invertibrates.
This document discusses regeneration in living organisms. It defines regeneration as the ability to replace or renew damaged or lost body parts after embryonic development. Regeneration involves growth, morphogenesis, and cell differentiation regulated by signaling pathways like WNT and FGF. There are three main types of regeneration: physiological regeneration which replaces regularly lost cells; reparative regeneration which repairs wounds or lost parts; and autotomy where animals self-detach parts when threatened. Regeneration abilities vary across vertebrates, from restricted tissue regeneration in mammals to full limb regeneration in salamanders and fish fin regeneration. The process of limb regeneration occurs in three phases: wound healing, blastema formation from progenitor cells, and redifferentiation of the blastema into
Organisms have developed various adaptations to light levels in their environments. The eye can adjust to different light intensities through a process called light adaptation. For humans, sunlight provides vitamin D and regulates biological rhythms, but too much sun can cause sunburn or skin cancer from overexposure. Fish also have circadian rhythms influenced by light and require a few hours of light per day to regulate functions. Some fish produce their own light through bioluminescence. Plants rely completely on photosynthesis to use sunlight to produce sugars for food and energy, which they store in leaves to share with other organisms. Without light, plants cannot grow due to an inability to produce necessary energy.
The document discusses different types of animal adaptations including structural, protective coloration, mimicry, and behavioral adaptations such as migration and hibernation. Structural adaptations involve parts of an animal's body like teeth and coverings. Protective coloration allows animals to blend in through camouflage. Mimicry allows animals to resemble others to fool predators. Behavioral adaptations include migration to find better environments and hibernation where animals slow their body functions to conserve energy.
The evolutionary history of camels began around 50 million years ago with their small, rabbit-sized ancestor called Protylopus in North America. Protylopus had adaptations like shorter front limbs that allowed it to browse for leaves. Changing climates led to species like Poebrotherium that were larger with tooth and leg adaptations for grasslands. Procamelus emerged during cooling periods and migrated. Paracamelus developed in the Pliocene with modern features. Camelus emerged in the Pleistocene as one-humped dromedaries in deserts. Modern camels have adaptations like humps and eyelashes for harsh desert environments.
Biological collections preserve plant and animal specimens through various methods. Dry collections involve preserving specimens without liquid through rigidity or highlighting distinguishing features. Wet collections submerge specimens in liquid preservatives to maintain body form and soft tissues. Low-temperature collections maintain specimens' viability for analysis by storing at cold temperatures. Microscopy collections prepare specimens for examination under microscopes. Proper collection, preservation, cataloging and storage help museums maintain valuable reference materials.
TO FOLLOW THESE SLIDES you will learn about the adaptive radiations involve in evolution .
yo will learn about the parallel adaptations and its types
speciation role in the evolution
factors
key innvations
to imrove the article involving examples
Founder events
Adaptive plasticity
process of adaptive radiation
Factors promote adaptive radiations
Factors underlying adaptive radiations
defined by 0.S OSBORN
ecological space
geological
climatological
Islands
examplrs: 1.Darwin Finches 2.Cichlid fish genome -adaptive evolution, Stanford scientists
3.Anolis Lizards
Factors promote adaptive radiations
1.Generally speaking, adaptive radiations occur when new, unoccupied ecological niches become accessible to a founder population.
This can happen after a mass extinction during which the previous occupiers of those niches died out.
t can also happen when a colonizing species arrives at an island. (For instance the ancestor of the honeycreepers in Hawaii, or of Darwin's "finches" in the Galapagos)
Honey creeper
Change feeding habitat
At least 56 species of Hawaiian honeycreepers known to have existed, although all but 18 of them are now extinct.
Lack of competition. When a species enters an adaptive zone, it is poorly equipped to compete with species that have become adapted to the same niche.
For example, mudskippers are fish that are making a living on land, but they are marine fish and they don't have to compete against frogs and salamanders, which are restricted to fresh water. That is why we don't see freshwater mudskippers.
process of adaptive radiation
Ecological Release Colonization of species.
Taxon cycle
Habitat varying as population expand- species dispersal.
Adaptive plasticity Phenotypic plasticity(behavior change)
Property of an individual or genotype that may be adaptive, maladaptive or neutral with regard to an individual's fitness.
The particular way an individual's (or genotype's) phenotype varies across environments can be described as a reaction norm (Single genotype-phenotypic expression)
Speciation in adaptive radiation Founder events
This document discusses nitrogenous waste and endogenous chemicals in fish tissues. It explains that nitrogenous wastes are synthesized from excess amino acids and must be excreted. The three main types of nitrogenous waste used by vertebrates are ammonia, urea, and uric acid. Endogenous chemicals in fish tissues include fatty acids and eicosanoids. Fatty acids are precursors for eicosanoids, which have many physiological functions. Eicosanoids include prostaglandins, thromboxanes, and leukotrienes.
ORIGIN OF CHORDATES
Animal kingdom is basically divided into two sub kingdoms:
Non-chordata- including animals without notochord.
Chordata- This comprising animals having notochord or chorda dorsalis.
Chordates were evolved sometime 500 million years ago during Cambrian period (invertebrates were also began to evolve in this period) .
Chamberlain (1900) pointed out that all modern chordates possess glomerular kidneys that are designed to remove excess water from body.
It is believed that Chordates have originated from invertebrates.
It is difficult to determine from which invertebrate group the chordates were developed.
Chordate ancestors were soft bodied animals. Hence they were not preserved as Fossils.
However, early fossils of chordates have all been recovered from marine sediments and even modern protochordates are all marine forms.
Also glomerular kidneys are also found in some marine forms such as myxinoids and sharks. That makes the marine origin of chordates more believable.
Chordates evolved from some deuterostome ancestor (echinoderms, hemichordates, pogonophorans etc.) as they have similarities in embryonic development, type of coelom and larval stages.
Many theories infers origin of chordates, hemichordates and echinoderms from a common ancestor.
This document discusses taxonomic characters which are attributes used to classify organisms based on similarities and differences. It covers the importance of characters in classification, their characteristics, types including morphological, physiological, behavioral, ecological and geographic. It also discusses the role of characters in modern taxonomy, character weighting, and inadmissible characters that are excluded from classification like meaningless, logically correlated, partially correlated and invariant characters.
This document discusses different types of biological variations that can occur in animals, including genetic, non-genetic, individual, social, ecological, traumatic, age-related, seasonal, and polyphenic variations. It provides examples of variations in appearance between juvenile and adult stages, between seasons, and in response to environmental conditions. It also summarizes variations that can occur between sexes, castes in social insects, populations in different habitats, in response to atypical environmental conditions, and those that involve allometric growth or neurogenic color changes.
The document discusses neutral theory of molecular evolution, which holds that most genetic changes are due to neutral mutations that do not affect organismal fitness. It proposes that neutral mutations accumulate over time at a constant rate, allowing relative divergence times to be estimated. The theory aims to explain high genetic variation and presence of neutral substitutions between species. Several lines of evidence are presented, including comparative rates of evolution between functionally important and unimportant genes and gene regions.
Taxonomic collection and identificationAftab Badshah
Biological collections are valuable for preserving extinct species and rare specimens. They allow researchers to verify original data and study specimens that are otherwise inaccessible. Effective collections involve sampling populations across species' ranges and variations, including larvae and parasites. Specimens are collected using various methods and preserved appropriately through methods like alcohol, stuffing, or formalin depending on the taxon. Proper long-term labeling with data like location, date, life stage, and collector is critical for research.
Zoological nomenclature establishes scientific names for animal taxa according to a set of international rules to ensure names are unique, universal, and stable, with each taxon having a designated type specimen to serve as the objective standard for applying its name. The principle of priority dictates that the oldest available name for a taxon is the valid name, while the principle of the first reviser resolves situations where two names have the same date. Names apply to both living and extinct animals according to these principles and rules.
Evolutionary biologists use phylogenetic trees and cladistics to study evolutionary relationships between organisms and construct classifications. Cladistics involves analyzing shared characteristics to hypothesize how groups of organisms evolved from common ancestors over time. A key assumption is that all organisms are related through descent from a shared ancestor. Cladograms graphically represent evolutionary relationships, with shared derived characteristics defining monophyletic clades. Parsimony is used to select the simplest phylogenetic tree that is best supported by evidence.
Orthogenesis is the theory that organisms evolve in a definite direction due to some internal mechanism, rejecting natural selection. Allometry describes the relationship between an organism's size and its body parts, such as brain size increasing with body size. Adaptive radiations occur when environmental changes open new niches, causing rapid speciation and phenotypic adaptation in a relatively short time, as seen with Hawaiian honeycreepers adapting to different island environments.
This document discusses evolutionary theories around sexual selection and relationships. It explains that sexual selection occurs through intrasexual competition between males to attract females, and intersexual selection where females choose their mates. Research by Buss (1989) found cross-cultural differences in mate preferences, with females prioritizing resources and males prioritizing youth and attractiveness. Studies also show females' preferences change across their menstrual cycle and they favor more masculine traits when fertile. However, critics argue these theories are reductionist and deterministic by not accounting for free will or environmental influences on relationships.
1. Microevolution leads to genetic differences between populations over time, which can result in new species through macroevolution if enough differences accumulate.
2. A polytypic species consists of multiple geographically isolated populations that have undergone microevolution, like the different tiger subspecies.
3. Recognizing polytypic species simplifies classification by reducing many similar local populations to subspecies rather than considering each a unique species. However, delineating subspecies from one another or related species remains challenging.
To determine the variation and the limitation between species, many concepts have been proposed.
When a taxonomist study a particular taxa, he/she must adopted a species concept and provide a species limitation to define this taxa.
Plant kingdom as other living kingdoms has a hierarchy structure ends mostly with species rank.
Species are one of the basic units to compare in almost all fields of biology.
A species is defined as the largest group of organisms in which two individuals are capable of reproducing fertile offspring, typically using sexual reproduction.
Definition of a species as a group of interbreeding individuals cannot be easily applied to organisms that reproduce only or mainly asexually.
If two lineages of oak look quite different, but occasionally form hybrids with each other, should we count them as different species?
Idea of a species is something that we humans invented for our own convenience.
‘‘No matter what variations occur in the individuals or the species, if they spring from the seed of one and the same plant, they are accidental variations and not such as distinguish a species permanently; one species never springs from the seed of another nor vice versa” - JOHN RAY.
Used a sexual system ‘‘natural system” for defining species - LINNAEUS.
‘‘A species is a collection of all the individuals which resemble each other more than they resemble anything else, which can by natural fecundation produce fertile individuals, and which reproduce themselves by generation, in such a manner that we may from analogy suppose them all to have sprung from one single individual” - DE CANDOLLE.
This document provides a history of taxonomy from ancient times through the modern era. It discusses how early ancient Chinese, Egyptian, Greek, and Roman scholars began classifying and organizing plants and animals. Key early taxonomists mentioned include Aristotle, Theophrastus, Dioscorides, and Pliny. It then covers the contributions of later taxonomists like Caesalpino, the Bauhin brothers, John Ray, and Joseph Pitton de Tournefort. The era of Carl Linnaeus is described as revolutionizing taxonomy through his introduction of binomial nomenclature. The document outlines how Linnaeus helped transform botany and zoology into scientific disciplines. It concludes with a discussion of developments like cl
The document discusses several trends and rates of evolution, including Dollo's law of irreversibility, Cope's law of body size increase over generations, and Gause's exclusion principle of ecological niche overlap. It also covers Bergman's rule relating body size and temperature, techniques for examining evolutionary rates like DNA analysis, and the hypotheses of gradual vs punctuated change to explain rates of evolution. Resources on these topics are listed at the end.
Physiology of Respiration in InvertebratesPRANJAL SHARMA
In physiology, respiration is the movement of oxygen from the outside environment to the cells within tissues, and the removal of carbon dioxide in the opposite direction. In these slides you will get to know about Physiology of Respiration in Invertibrates.
This document discusses regeneration in living organisms. It defines regeneration as the ability to replace or renew damaged or lost body parts after embryonic development. Regeneration involves growth, morphogenesis, and cell differentiation regulated by signaling pathways like WNT and FGF. There are three main types of regeneration: physiological regeneration which replaces regularly lost cells; reparative regeneration which repairs wounds or lost parts; and autotomy where animals self-detach parts when threatened. Regeneration abilities vary across vertebrates, from restricted tissue regeneration in mammals to full limb regeneration in salamanders and fish fin regeneration. The process of limb regeneration occurs in three phases: wound healing, blastema formation from progenitor cells, and redifferentiation of the blastema into
Organisms have developed various adaptations to light levels in their environments. The eye can adjust to different light intensities through a process called light adaptation. For humans, sunlight provides vitamin D and regulates biological rhythms, but too much sun can cause sunburn or skin cancer from overexposure. Fish also have circadian rhythms influenced by light and require a few hours of light per day to regulate functions. Some fish produce their own light through bioluminescence. Plants rely completely on photosynthesis to use sunlight to produce sugars for food and energy, which they store in leaves to share with other organisms. Without light, plants cannot grow due to an inability to produce necessary energy.
The document discusses different types of animal adaptations including structural, protective coloration, mimicry, and behavioral adaptations such as migration and hibernation. Structural adaptations involve parts of an animal's body like teeth and coverings. Protective coloration allows animals to blend in through camouflage. Mimicry allows animals to resemble others to fool predators. Behavioral adaptations include migration to find better environments and hibernation where animals slow their body functions to conserve energy.
Control of body temperature and water balance (copy)Mae Canencia
This document discusses thermoregulation and osmoregulation in animals. Thermoregulation is the process by which animals maintain an internal temperature within a tolerable range through various adaptations. These include metabolic heat production, insulation, circulatory adaptations, evaporative cooling, and behavioral responses. Osmoregulation is the homeostatic control of water and solute levels. The kidneys and urinary system play a key role in osmoregulation and homeostasis by filtering blood to form urine and regulating water and solute levels in the body through filtration, reabsorption, secretion and excretion.
The document discusses structural adaptations that help animals move efficiently. It explains that streamlined bodies help aquatic animals like fish move fast through water to catch prey or escape predators. Fish also have modified limbs and swim bladders to aid movement and buoyancy. The document then discusses adaptations for flight, noting that flying animals are generally lightweight with large, wing surfaces, streamlined shapes, and strong flight muscles. Birds in particular have feathers, hollow bones, and streamlined bodies to reduce air resistance and fly efficiently.
Adaptations For Breathing In Water (2 Jul)Rizal Jailani
The document discusses various anatomical adaptations that aquatic animals have evolved for breathing underwater. It describes how fish, amphibians, insects, and mammals each breathe using structures like gills, skin, air tubes, air bubbles, and specialized nostrils or blowholes. These adaptations allow animals to extract oxygen from water or bring air underwater to survive in their aquatic environments.
Powerpoint presentation-Adaptation Of Living Organisms In Its EnvironmentHyacinth B.L.Bensi
This document discusses different types of adaptations that help organisms survive in their environments. It focuses on adaptations in animals and plants. There are two main types of adaptations - structural adaptations, which involve physical characteristics like coloration, size or shape of body parts; and behavioral adaptations, which refer to how organisms act and behave. Examples of structural adaptations in animals include camouflage, mimicry and body structures. Behavioral adaptations in animals include migration, hibernation and hunting in packs. Structural adaptations in plants involve physical structures that store water or catch minerals. Seeds also have adaptations to help them survive and grow. All living things adapt in some way to help them thrive in their environment.
Adaptations for getting and chomping food (teach)Moira Whitehouse
The document discusses animal adaptations for obtaining and eating food. It describes how herbivores, carnivores, and omnivores have different adaptations based on their diets. Herbivores have teeth for grinding plants, carnivores have sharp teeth for catching and tearing meat, and omnivores have adaptations for both plant and meat consumption. Examples are given of teeth, jaws, and other features in various species that help with getting and processing different types of food.
This document discusses animal adaptations, separating them into two categories: physical and behavioral. Physical adaptations are body structures like camouflage, mimicry, body coverings, and chemical defenses that help animals survive. Behavioral adaptations are animals' actions that can be instinctive, like finding shelter or raising young, or learned through interacting with the environment. Together, physical and behavioral adaptations allow animals to find food, defend themselves, and reproduce in their environments.
This document discusses the adaptations of several aquatic animals including dolphins, sea turtles, orca whales, and sea lions. Dolphins have streamlined bodies, flippers, thick blubber, and use echolocation to hunt. Sea turtles have a shell for protection, strong flippers for swimming, and can stay submerged for long periods. Orca whales are highly social, hunt in pods, and have a varied diet including fish, seals, and even other whales. Sea lions have flippers they use to walk and swim, thick blubber, and inhabit cooler northern waters where they feed on fish and squid. All of these animals have developed characteristics that allow them to thrive in their aquatic environments
This document discusses using ancient DNA analysis to study archaeological remains. It notes that ancient DNA is typically fragmented into small pieces 100-500 base pairs long. Contamination from other sources is also a major issue. However, ancient DNA analysis can be used to study species phylogenies, hominin evolution, past diets and behaviors, origins of domestication, and population histories. As a case study, the document discusses analyzing ancient DNA from pygmy hippopotamus remains on Cyprus to learn about population dynamics and what caused their extinction 12,000 years ago alongside human arrival and climate change. Stable isotope analysis of bones and teeth can also provide clues about past diets.
The document discusses three topics related to the documentary "Home":
1. It asks readers to explain one of the environmental problems mentioned in the documentary and propose a sustainable solution.
2. It asks readers to mention an example of sustainable development shown at the end of the documentary.
3. It asks readers to describe at least two statistical data points that are described in the documentary.
The Ecology and Evolution of Seaweed DiversificationHeroen Verbruggen
The document discusses species diversity and taxonomy of the algal order Dictyotales. It provides background on patterns of species diversification through speciation and extinction. It then summarizes ongoing research using DNA barcoding and phylogenetic analysis of Dictyotales species to understand their biogeographic patterns of diversification, including determining origins and dispersal routes of taxa. Models of evolutionary change are being used to analyze diversification patterns within the order.
Dissertation: Modelling fish dispersal in catchments affect by multiple anthr...jradinger
Presentation for the defense (disputation) of the doctoral thesis (dissertation) entitled: "Modelling fish dispersal in catchments affect by multiple anthropogenic pressures" submitted by Johannes Radinger at Humboldt-Universität zu Berlin, Germany. Date: Nov 19, 2014.
Evolutionary Genetics by: Kim Jim F. Raborar, RN, MAEd(ue)Kim Jim Raborar
This presentation was created as a partial fulfillment of the requirements in the subject Advanced Genetics. Everything that was here were kinda symbolic. I mean, you could recognize that this was a product of so much data interpretation. I therefore suggest you read and read a lot first before you go back to this presentation. Or you could just contact me so i could send you the key-pointers.
Have a super nice day.
Kimy
Seminar abstract: I will be talking about two ongoing research projects in my laboratory: (1) evolution of thermal niches in seaweeds, (2) biodiversity of endolithic algae in coral skeletons and its relationship with the environment. Using evolutionary models in an explicit phylogenetic framework, patterns of evolution in environmental traits such as the sea surface temperature (SST) affinities of species can be studied. Based on case studies in the green algae Codium and Halimeda, it is shown that lineages behave differently when it comes to their evolution of SST affinities, and that there is a strong correlation between the evolution of SST affinities and rates of species diversification. For the second part of the talk, I will focus on our recent work on environmental sequencing of coral skeletons. These feature unexpectedly high biodiversity of limestone-boring algae as well as many unknown inhabitants. Our first results indicate that the diversity of algal endoliths may be linked to environmental conditions, but this hypothesis needs further testing.
Marine Host-Microbiome Interactions: Challenges and OpportunitiesJonathan Eisen
This document summarizes a talk given by Jonathan Eisen on marine host-microbiome interactions. It discusses various topics researched in Eisen's lab, including phylogenomic methods and tools, microbial phylogenomics and evolvability, reference data resources, communication in science, and model systems. Specific projects are mentioned, such as automated genome trees, phylogenetic marker genes, the GEBA project, and dark matter microbes. The document then introduces the concept of the host-microbiome stress triangle and gives examples of stress types including nutrient acquisition, pathogens, and environmental change. It concludes by discussing a potential project on seagrass microbiomes in collaboration with Jay Stachowicz's lab.
Generalizing phylogenetics to infer patterns of shared evolutionary eventsJamie Oaks
This document discusses generalizing phylogenetics to infer patterns of shared evolutionary events. It begins by outlining how shared ancestry is a fundamental property of life and how phylogenetics is progressing as the statistical foundation of comparative biology. It then discusses how violating the assumption of independent divergences can provide insights into processes like biogeography, gene family evolution, and endosymbiont evolution. The document considers challenges in accounting for shared divergences, like the complexity of gene trees and sampling over all possible models, and proposes two approaches: using existing methods or approximate Bayesian computation with a diffuse Dirichlet process prior.
"The Quest for A field Guide to the Microbes" talk by Jonathan Eisen February...Jonathan Eisen
The document discusses the author's quest to create a field guide to microbes. It describes the challenges in doing so given microbes' small size and high diversity. The author discusses using DNA sequencing and phylogenetic trees to identify microbes and determine their functions and relationships. Examples are given of using DNA to study human microbiomes, forensic analysis, and microbial communities. The need for a comprehensive field guide is argued to better understand the roles and identification of microbes.
1. The document discusses applications of DNA in forensic science, including DNA profiling, prenatal paternity testing, and paternity testing.
2. It also discusses using DNA analysis to determine elements of biological evidence to solve crimes and legal disputes. DNA testing provides certainty that helps law enforcement and influences society.
3. The document then discusses population evolution and microbial life, biological diversity evolution, plant and animal development, population growth, and biomes and ecosystems.
This document provides an overview of key concepts in evolution science. It discusses Jean Baptiste de Lamarck as the first evolutionist and his three theories of evolution: need, use and disuse, and acquired characteristics. It also covers Charles Darwin's theory of natural selection and how it led to the evolution of giraffes' long necks. The document then examines mechanisms of evolution like mutation, genetic drift, gene flow and natural selection. It defines speciation and the different types like allopatric, peripatric, parapatric and sympatric speciation. In the end, it provides a review questions and short test on these evolutionary concepts.
This document discusses evolution and the forces that drive it. It defines evolution as changes in heritable traits over generations of a population. The main forces that cause evolution are genetic drift, genetic recombination, mutation, gene flow, and natural selection. It also discusses evidence that supports evolution such as the fossil record, comparative anatomy, and molecular biology. Finally, it covers different theories of evolution including Lamarck's theory, Darwin's theory of natural selection, and the modern synthesis theory.
The document provides study materials for Mrs. Ulry's Biology 202B class, including a review of key concepts from four class units and a 20 question multiple choice exam covering those units. It reviews differences between DNA and RNA, genetic processes like transcription and translation, evolutionary concepts like natural selection and genetic drift, and taxonomy. Sample review questions are provided to help students prepare for the exam.
The document summarizes a study on population genomics of a pipefish species. The study found:
1) Neutral processes like genetic drift primarily drive genetic differentiation between marine populations, though some evidence of local adaptation was found. Phenotypic differentiation followed a different pattern.
2) Freshwater populations were genetically distinct from each other and saltwater populations, indicating adaptation to freshwater. Many significant loci were shared among freshwater populations, suggesting a similar genetic basis of adaptation.
3) Neighboring saltwater populations did not share significant SNPs, while over 50 loci were outliers in comparisons of all freshwater population pairs, with over 30 in annotated gene regions. This supports local adaptation to freshwater environments.
This document summarizes research on human genetic population structure and diversity. The key points are:
- 85% of human genetic variation exists within populations, 10% among continental groups, and 5% among populations within the same continent.
- Clustering analyses of genetic data yield inconsistent groupings depending on the traits or markers used, and populations form a continuous gradient without clear boundaries.
- The patterns of genetic diversity are consistent with an origin of modern humans in Africa followed by serial founder effects during dispersal, around 56,000 years ago.
UC Davis EVE161 Lecture 17 by @phylogenomicsJonathan Eisen
This document contains slides from a lecture on metagenomics given by Jonathan Eisen at UC Davis in winter 2014. The lecture discusses shotgun metagenomics and analyzing metagenomic functions and gene content from environmental samples without genome assemblies. It provides an example of a comparative metagenomics study of various microbial communities that identified habitat-specific genes and metabolic profiles reflecting the different environments. The slides include figures and references from a 2005 Science paper on this topic. Problem set 4 for the class involves selecting a relevant paper for presentation the following week.
PowerPoint presentation that highlights chapters 13 and 14 in Campbell's Essential Biology (3rd. edition). It can also be used for Miller & Levine's Biology (2006 Ed.) for chapters 15-18.
This document summarizes key statistics on biodiversity and extinction rates:
- It lists the estimated numbers of various taxonomic groups ranging from protists to mammals.
- It notes that 99% of all species that have ever existed are now extinct, and current extinction rates are 100-10,000 times higher than background rates.
- Several studies are cited showing high percentages of threatened species for groups like mammals, amphibians, birds, sharks and corals.
- Causes of extinction are discussed, including habitat destruction, overexploitation, invasive species, climate change and their interactions.
Similar to Cave animals at the dawn of speleogenomics (20)
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
The binding of cosmological structures by massless topological defectsSérgio Sacani
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...AbdullaAlAsif1
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
The debris of the ‘last major merger’ is dynamically youngSérgio Sacani
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...Sérgio Sacani
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
1. Cave animals
at the dawn of speleogenomics
Markus Friedrich
Wayne State University
friedrichwsu@gmail.com
2. Cave animals
at the dawn of speleogenomics
● Spelling correct?
○ I think so
○ Spelleochecked this morning...
3. Cave animals
at the dawn of speleogenomics
● What does it mean?
○ Concepts and approaches
4. Cave animals
at the dawn of speleogenomics
● Transcriptome or genome sequence based analyses of
cave faunas:
■ Viruses
■ Bacteria
■ Algae
■ Plants
■ Obligate cave animals
● Extant
● Extinct
5. Cave animals
at the dawn of speleogenomics
● Transcriptome or genome sequence based analyses of
cave faunas:
■ Specimen samples
■ Environmental samples
6. Cave animals
at the dawn of speleogenomics
● Transcriptome or genome sequence based analyses of:
■ Regressive traits
Protas & William R. Jeffery WIREs Dev Biol 2012. doi: 10.1002/wdev.61
7. Cave animals
at the dawn of speleogenomics
● Transcriptome or genome sequence based analyses of:
■ Regressive traits
■ Relaxed vs positive selection
Protas & William R. Jeffery WIREs Dev Biol 2012. doi: 10.1002/wdev.61
8. Cave animals
at the dawn of speleogenomics
● Transcriptome or genome sequence based analyses of:
■ Regressive traits
■ Relaxed vs positive selection
■ Constructive (elaborated) traits
Protas & William R. Jeffery WIREs Dev Biol 2012. doi: 10.1002/wdev.61
9. Cave animals
at the dawn of speleogenomics
● Transcriptome or genome sequence based analyses of:
■ Regressive traits
■ Relaxed vs positive selection
■ Constructive (elaborated) traits
■ Convergent vs parallel evolution
Protas &
William R.
Jeffery
WIREs Dev
Biol 2012.
doi:
10.1002/wd
ev.61
10. Candidate gene studies harvesting
molecular key signatures of the evolutionary process
○ Crandall, K. A., & Hillis, D. M. (1997). Rhodopsin evolution in the dark. Nature,
387(6634), 667–668.
○ Buhay, J. E., & Crandall, K. A. (2005). Subterranean phylogeography of
freshwater crayfishes shows extensive gene flow and surprisingly large
population sizes. Molecular Ecology, 14(14), 4259–4273.
○ Leys, R., Cooper, S. J. B., Strecker, U., & Wilkens, H. (2005). Regressive
evolution of an eye pigment gene in independently evolved eyeless
subterranean diving beetles. Biology Letters, 1(4), 496–499.
○ Aspiras, A. C., Prasad, R., Fong, D. W., Carlini, D. B., & Angelini, D. R. (2012).
Parallel reduction in expression of the eye development gene hedgehog in
separately derived cave populations of the amphipod Gammarus minus.
Journal of Evolutionary Biology, 25(5), 995–1001.
○ Niemiller, M. L., Fitzpatrick, B. M., Shah, P., Schmitz, L., & Near, T. J. (2013).
Evidence for repeated loss of selective constraint in rhodopsin of amblyopsid
cavefishes (Teleostei: Amblyopsidae). Evolution; International Journal of
Organic Evolution, 67(3), 732–748.
11. Speleogenomic studies harvest
molecular signatures of evolutionary change
○ Cave-adaptive loss of function:
■ Ortholog of surface genes not detectable
Gene x
12. ○ Cave-adaptive loss of function:
■ Ortholog of surface genes not detectable
Gene x
Speleogenomic studies harvest
molecular signatures of evolutionary change
13. ○ Cave-adaptive loss of function:
■ Orthologs of surface genes not detectable
■ Transcripts with deletions, nonsense or frameshift
mutations
Gene x
Gene x
Speleogenomic studies harvest
molecular signatures of evolutionary change
14. ○ Cave-adaptive loss of function:
■ Orthologs of surface genes not detectable
■ Transcripts with deletions, nonsense or frameshift
mutations
■ Equal numbers of replacement (dN) vs silent
substitutions (dS) mutation ratio (dN/dS = 1)
Gene x
Gene x
dN/dS = 1
dN/dS < 0.2
Speleogenomic studies harvest
molecular signatures of evolutionary change
15. ○ Cave-adaptive gain of function:
■ Orthologs of surface genes expressed at higher level
Gene x
Gene x
Speleogenomic studies harvest
molecular signatures of evolutionary change
16. ○ Cave-adaptive gain of function:
■ Orthologs of surface genes expressed at higher level
Gene x
Gene x
Speleogenomic studies harvest
molecular signatures of evolutionary change
17. ○ Cave-adaptive gain of function:
■ Orthologs of surface genes expressed at higher level
■ Gene family expansions compared to surface forms
Gene x
Gene x 1
Gene x 2
Speleogenomic studies harvest
molecular signatures of evolutionary change
18. ○ Cave-adaptive gain of function:
■ Orthologs of surface genes expressed at higher level
■ Gene family expansions compared to surface forms
■ High replacement vs silent substitution ratio
Gene x
Gene x
dN/dS > 2
dN/dS < 0.2
Speleogenomic studies harvest
molecular signatures of evolutionary change
19. ○ Cave-adaptive gain of function:
■ Orthologs of surface genes expressed at higher level
■ Gene family expansions compared to surface forms
■ High replacement vs silent substitution ratio
■ Selective sweep patterns
Speleogenomic studies harvest
molecular signatures of evolutionary change
20. ○ Purifying selection:
■ Orthologs of surface genes intact
■ Expressed at similar levels
■ Low replacement vs silent substitution ratio
Speleogenomic studies harvest molecular signatures of
evolutionary change or conservation
Gene x
Gene x
dN/dS < 0.2
dN/dS < 0.2
21. ○ Cave-adaptive loss of function
■ Orthologs of surface genes not detectable
■ Transcripts with deletions, nonsense or frameshift
mutations
■ Equal replacement vs silent substitution mutation ratio
○ Cave-adaptive gain of function
■ Orthologs of surface genes expressed at higher level
■ Gene family expansions compared to surface forms
■ High replacement vs silent substitution ratio
■ Selective sweep patterns
○ Purifying selection
○ All of the above identify candidate trait changes
-> Predictive genomics across >5,000 genes
From sequence read to trait discovery:
Predictive speleogenomics
22. Cave animals
at the dawn of speleogenomics
● Spelling correct
● Transcriptome or genome sequence based analyses of
cave adaptation
● What have we learned so far?
23. Welcome
to the age of Next Generation Sequencing!
http://www.slideshare.net/AlagarSuresh/ngs-introduction-51560394/2
2005
28. Kim et al. (2011): Genome sequencing reveals insights into physiology and longevity of
the naked mole rat. Nature, doi:10.1038/nature10533
● Strictly subterranean
o Tolerate low oxygen and high carbon dioxide concentrations
● Eusocial
o Reside in large colonies with a single breeding female (queen), who
suppresses the sexual maturity of her subordinate.
● Extraordinarily long-lived (>30 years)
o Negligible senescence
▪ No age-related increase in mortality
▪ High fecundity until death
▪ Resistant to both spontaneous cancer and experimentally induced
tumorigenesis
● Unable to sustain thermogenesis
● Insensitivity to certain types of pain
● Microphthalmic
2005 2006 2007 2008 2009 2010 2011
29. ● 2,4 Gb large genome
● 22,500 genes
● Apparent lack of age related expression level changes
● 39 Proteins with unique amino acid changes
compared to 36 vertebrate genomes
○ Thermoregulation
○ DNA repair
○ Oncogenesis
○ Vision
2005 2006 2007 2008 2009 2010 2011
Kim et al. (2011): Genome sequencing reveals insights into physiology and longevity of
the naked mole rat. Nature, doi:10.1038/nature10533
30. ● Pain reception related neuropeptide gene ACT1
intact but ancestral promoter missing
● 244 pseudogenes:
○ 183 frameshift events
○ 119 premature termination events
Functional categories enriched for
pseudogenes:
Spermatogenesis
Olfactory receptor activity
Visual perception
2005 2006 2007 2008 2009 2010 2011
Kim et al. (2011): Genome sequencing reveals insights into physiology and longevity of
the naked mole rat. Nature, doi:10.1038/nature10533
31. Kim et al. (2011): Genome sequencing reveals insights into physiology and longevity of
the naked mole rat. Nature, doi:10.1038/nature10533
2005 2006 2007 2008 2009 2010 2011
Lack of function mutations in visual system genes:
32. 2005 2006 2007 2008 2009 2010 2011
Friedrich et al. (2011): SB. 2011. Phototransduction and clock gene expression in the
troglobiont beetle Ptomaphagus hirtus of Mammoth cave. J Exp Biol 214:3532–41.
33. 2005 2006 2007 2008 2009 2010 2011
Friedrich et al. (2011): Phototransduction and clock gene expression in the troglobiont
beetle Ptomaphagus hirtus of Mammoth cave. J Exp Biol 214:3532–41.
Bottom
Surface
34. Academy
1839-1905
Alpheus Spring Packard 1888. The cave fauna of North America, with
remarks in the anatomy of brain and the origin of blind species.
Memoirs of the National Academy of Sciences (USA) 4, 1-156
35. Academy
Alpheus Spring Packard 1888. The cave fauna of North America, with
remarks in the anatomy of brain and the origin of blind species.
Memoirs of the National Academy of Sciences (USA) 4, 1-156
"Transverse and horizontal
sections of the head of
Adelops (=Ptomaphagus)
hirtus, from Mammoth
Cave, reveal no traces of
the optic ganglia or the
optic nerve."
36. Academy
Alpheus Spring Packard 1888. The cave fauna of North America, with
remarks in the anatomy of brain and the origin of blind species.
Memoirs of the National Academy of Sciences (USA) 4, 1-156
"Transverse and horizontal
sections of the head of
Adelops (=Ptomaphagus)
hirtus, from Mammoth
Cave, reveal no traces of
the optic ganglia or the
optic nerve."
"It thus appears that
Adelops must be blind
though the eyes exist in a
degenerate state."
40. RNA-seq: Deep sequencing transcriptome analysis
• NSF award NSF Award #0951886: Pax6 and the genetic regulation of eye
development in Tribolium
• Rui Chen, Bryce Daines (Baylor College)
41. 25 dissected P. hirtus heads minus antennae
Deep sequencing of the P. hirtus head transcriptome
• NSF award NSF Award #0951886: Pax6 and the genetic regulation of eye
development in Tribolium
• Rui Chen, Bryce Daines (Baylor College)
42. ● 27,428,409 short reads (75 bp) produced
● 5,476,803 (19.97%) aligned by blastx to Tribolium refseq proteins
● Orthologs of 8718 Tribolium transcripts observed with >10 reads
● Concatemerization of blastx hit group reads
Drosophila (Adams et al 2000)
Ptomaphagus
Tribolium (TGSC 2008)
100200300 Mya
Deep sequencing of the P. hirtus head transcriptome
43. The insect phototransduction protein machinery
R
opsin
Gqα
β
PLCβ PKC 53E
γ
INAD INAD
5
Arrestin 1
Arrestin 2
Gqα Gqα
R
opsin
R
opsin
PLCβ PKC 53E
2
TRP
4
3
1
5
2
4
3
1
Ca2+
44. The insect phototransduction protein machinery
R
opsin
Gqα
β
PLCβ PKC 53E
γ
INAD INAD
5
Arrestin 1
Arrestin 2
Gqα Gqα
R
opsin
R
opsin
PLCβ PKC 53E
2
TRP
4
3
1
5
2
4
3
1
Ca2+
45. Conserved expression of the insect phototransduction
protein machinery in P. hirtus
R
opsin
Gqα
β
PLCβ PKC 53E
γ
INAD INAD
5
Arrestin 1
Arrestin 2
Gqα Gqα
R
opsin
R
opsin
PLCβ PKC 53E
2
4
3
1
5
2
4
3
1
TRP
Ca2+
46. Transcript conservation predicts
a functional visual system in P. hirtus
•Structural evidence
•Behavioral evidence
Phototaxis
Circadian rhythm
47. Our first light-dark choice assay
Friedrich et al. (2011) The Journal of Experimental Biology 214: 3532-3541.
48. Light vs dark choice of P. hirtus in response to desktop
lamp illumination
Friedrich et al. (2011) The Journal of Experimental Biology 214: 3532-3541.
49. Speleogenomic approach revealed:
Conservation of functional vision
Presence of photoreceptor cells
Negative photoresponse
Differential wavelength specificity
Conservation of circadian gene expression
Preliminary evidence of circadian activity
rhythms
Genetic regression of the visual system:
Loss of UV opsin
Loss of eye specific pigmentation genes:
cinnabar
scarlet
white
2005 2006 2007 2008 2009 2010 2011
Friedrich et al. (2011): Phototransduction and clock gene expression in the troglobiont
beetle Ptomaphagus hirtus of Mammoth cave. J Exp Biol 214:3532–41.
50. 2005 2006 2007 2008 2009 2010 2011 2012
● Genome size: 174 Mb
● ~13,500 genes
● “Dark-fly”: Drosophila melanogaster maintained in constant darkness > 60 years =
1,500 years
○ Dark-fly produce more offspring in dark than in light
○ Preserved strong phototactic behavior and circadian locomotor rhythm
○ Nonsense mutation in the Rhodopsin7 (not expressed in eye)
○ Functional conservation of Rhodopsin 1-6 (all expressed in the eye)
Izutsu et al. (2012): Genome features of "Dark-fly", a Drosophila line reared long-term in
a dark environment. PLoS One. 7(3):e33288.
51. 2005 2006 2007 2008 2009 2010 2011 2012 2013
Hinaux et al (2011): De novo sequencing of Astyanax mexicanus surface fish and
Pachón cavefish transcriptomes reveals enrichment of mutations in cavefish putative eye
genes. PLoS One. 8(1):e53553.
Gross et al. (2011): An integrated transcriptome-wide analysis of cave and surface
dwelling Astyanax mexicanus. PLoS One. 8(2):e55659
52. 2005 2006 2007 2008 2009 2010 2011 2012 2013
● 44,145 contigs assembled from expressed
sequence tags generated by Sanger sequencing
● 8 eight cDNA libraries
■ 4 from a surface stream population
■ 4 from the Pachon cave population
● For both populations, libraries were
generated from four developmental
stages.
Hinaux et al (2011): De novo sequencing of Astyanax mexicanus surface fish and
Pachón cavefish transcriptomes reveals enrichment of mutations in cavefish putative eye
genes. PLoS One. 8(1):e53553.
53. 2005 2006 2007 2008 2009 2010 2011 2012 2013
Hinaux et al (2011): De novo sequencing of Astyanax mexicanus surface fish and
Pachón cavefish transcriptomes reveals enrichment of mutations in cavefish putative eye
genes. PLoS One. 8(1):e53553.
● 31 non-conservative amino-acid changes specific to the
lineage of the cave population.
○ The significant majority function in carbohydrate
metabolism
○ No enrichment of eye developmental genes
● 79 non-conservative amino-acid differences between the
genes of surface and cave morphotypes tentative
evidence of suggested accelerated evolution of vision
genes
54. 2005 2006 2007 2008 2009 2010 2011 2012 2013
Gross et al. (2011): An integrated transcriptome-wide analysis of cave and surface
dwelling Astyanax mexicanus. PLoS One. 8(2):e55659
● 22,596 contigs from 454 sequencing data from Pachon
and surface animals
○ > 600 transcripts were unique to cave or surface
populations
■ 10% of which were related to genes known from
zebrafish
○ Two classes of gene functions overrepresented in
the conserved population-specific subsamples:
■ Metabolic genes were specific for, or
transcriptionally elevated in cave morphotype
■ 16 homologs associated with visual functions
specifically missing or reduced in the cave
morphotype
55. 2005 2006 2007 2008 2009 2010 2011 2012 2013
Meng et al. (2013): Evolution of the eye transcriptome under constant darkness in
Sinocyclocheilus cavefish. Molecular Biology and Evolution 30 :1527-1543
● Compared the mature retinal histology of surface and cave
species
○ Reduction in number and length of photoreceptor cells
● Generated transcriptomes for surface and cave species
● Transcriptional downregulation of eye fate promoting
transcriptional factors
56. 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016
Yang et al. (2016): The Sinocyclocheilus cavefish genome provides insights into cave
adaptation. BMC Biol. 2016 Jan 4;14:1.
●Whole-genome sequencing and comparative analysis of:
○ Macrophthalmous S. grahami, 1.75 Gb
○ Micophthalmous S. rhinocerous 1.73 Gb
○ Anophthalmous S. anshuiensis.and 1.68 Gb
●~40,000 genes in each species
●Several opsin genes missing in all three species:
○ Lws2, Rh2-1 and Rh2-2 (middle wavelength-sensitive)
57. 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016
Yang et al. (2016): The Sinocyclocheilus cavefish genome provides insights into cave
adaptation. BMC Biol. 2016 Jan 4;14:1.
● Anophthalmous S. anshuiensis:
○ Genetic regression related to pigmentation:
■ Reduced transcript levels of Oca2 and melanogenesis
pathway genes
■ Loss of Mpv17
○ Genetic regression related to vision:
■ Opsin gene Rh2-4 lost specifically in anophthalmic species
(Sa)
■ Nine eye developmental regulators transcriptionally
downregulated
58. 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016
Yang et al. (2016): The Sinocyclocheilus cavefish genome provides insights into cave
adaptation. BMC Biol. 2016 Jan 4;14:1.
● Anophthalmous S. anshuiensis:
○ Gain of function changes related to taste reception:
■ Duplication of taste receptor genes, such as Tas1r1 and
Tas2r200-2
○ Gain of function changes related to taste reception:
■ Hsp90α1
● Duplicated
● Higher expression level
59. 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014
Tierney et al. (2013): Opsin transcripts of predatory diving beetles: a comparison of
surface and subterranean photic niches. R Soc Open Sci. 2015 Jan 28;2(1):140386.
60. 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014
● Identified transcripts for UV, LW, and c-opsins
from surface beetle transcriptomes
● 3 out of 3 subterranean beetles presented
evidence of parallel loss of all opsin transcription
● Documents genetic regression of the visual
system
Tierney et al. (2013): Opsin transcripts of predatory diving beetles: a comparison of
surface and subterranean photic niches. R Soc Open Sci. 2015 Jan 28;2(1):140386.
61. 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014
McGaugh et al. (2014): The cavefish genome reveals candidate genes for eye loss. Nat
Communications 5:5307. doi: 10.1038/ncomms6307
● First de novo genome assembly for Astyanax mexicanus
○ ~1 Gb large genome
○ ~23,000 genes
■ ~16,000 1:1 orthologs to zebrafish
● Identifies candidate genes underlying previously mapped quantitative trait
loci (QTL)
62. 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014
McGaugh et al. (2014): The cavefish genome reveals candidate genes for eye loss. Nat
Communications 5:5307. doi: 10.1038/ncomms6307
● Identifies and tests developmental eye reduction candidate genes
● Assays cave adaptive candidate genes for potential functional and
expression differences between surface and cave morphotype
○ RT-PCR experiments define morphotype candidate gene losses as
false negatives
63. 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014
McGaugh et al. (2014): The cavefish genome reveals candidate genes for eye loss. Nat
Communications 5:5307. doi: 10.1038/ncomms6307
● RT-PCR confirmation of species-generic candidate gene losses in several
gene families:
○ Retinol dehydrogenases
○ Crystallins
○ Sine oculis homeoboxes
○ Opsins
○ Fibroblast growth factors
○ Gamma-aminobutyric acid A
○ Dopamine receptors
Vision
Sleep and circadian clock
64. 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015
Stahl et al. (2015): A Transcriptomic Analysis of Cave, Surface, and Hybrid Isopod
Crustaceans of the Species Asellus aquaticus. PLoS One. 10:e0140484.
● Deep sequenced cDNA libraries
○ Cave morphotype
○ Surface morphotype
○ Hybrid
● ~25,000 transcripts
○ >4,000 with orthologs in “other” or non-model
species
● Identifies candidate morphotype-specific alleles
65. Welcome to the tree of speleogenomic animal
life!
Astyanax mexicanus Sinocyclocheilus
Heterocephalus
glaber
P. hirtus
Paroster
macrosturtensis
Asellus aquaticus
Homo n+s
67. 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016
Speleogenomic coverage
of cave animal species so far
68. Coverage of animal genomes so far
2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016
http://www.ncbi.nlm.nih.gov/genome/browse/
69. Cave animals
at the dawn of speleogenomics
● What have we learned so far?
○ A lot
● What is the future holding?
○ Genetic surveys of vanishing diversity
○ High resolution genetic analysis of cave invasion
○ High resolution analysis of troglomorphism
○ Value of close relationship to model organisms
○ Impact of genetic drift: population genomics
○ Impact of time: Young vs old troglobites
○ Quest for the oldest and the youngest troglobite...