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1
Breeding of
WHEAT
Triticum aestivum
(2n=6x=42)
Dr. Mamata Khandappagol
Assistant Professor (Contractual)
Department of Genetics and Plant Breeding
CoA, Chamarajanagara-571127
UAS, GKVK, Bengaluru
Dr. Mamata K, Dept. of GPB, Chamarajanagara
Introduction
• Wheat is a staple crop for a significant proportion of the world’s
population.
• Wheat is rich in carbohydrates, protein and essential vitamins and
minerals such as vitamins B and E, calcium and iron, as well as
fibre.
• It is world’s most widely cultured crop occupying 22%
cultivated areas.
• It is the major crop of USA, Canada and Asia, it is C3 crop not
well adapted to tropical and subtropical condition.
• As many as 25 species recognised in the world, only 3 species
namely, T. aestivum/vulgare (Bread wheat), T. durum (Macroni
wheat) & T. dicoccum (Emmer wheat) commercially grown in
India.
2Dr. Mamata K, Dept. of GPB, Chamarajanagara
History
3
• Cultivation of wheat began to spread beyond the
Fertile Crescent after about 8000 BC
• Spread of cultivated wheat started in the Fertile
Crescent about 8500 BC, reaching Greece, Cyprus
and India by 6500 BC, Egypt shortly after 6000 BC.
• The early Egyptians were developers of bread and the
use of the oven and developed baking into one of the
first large-scale food production industries-
Dr. Mamata K, Dept. of GPB, Chamarajanagara
4Dr. Mamata K, Dept. of GPB, Chamarajanagara
5
Dr. Mamata K, Dept. of GPB, Chamarajanagara
6
Dr. Mamata K, Dept. of GPB, Chamarajanagara
Origin
• Wheat has evolved from wild grasses.
• The centre of origin is South Asia.
• Large genetic variability is observed in
Iran, Isreal, and Bordering countires.
7
Ploidy level Origin
Diploid (2X) Asia minor
Tetraploid (4X) Abyssinia, North Africa
Hexaploid (6X) Central Asia
Dr. Mamata K, Dept. of GPB, Chamarajanagara
Region of wheat origin: Region of wheat cultivation:
8Dr. Mamata K, Dept. of GPB, Chamarajanagara
Global Wheat Production
9
Dr. Mamata K, Dept. of GPB, Chamarajanagara
10Dr. Mamata K, Dept. of GPB, Chamarajanagara
Wheat production in India
11Dr. Mamata K, Dept. of GPB, Chamarajanagara
Distribution:
• USA, Canada, Latin America, Europe, China,
Japan, Argentina, Mexico, India, Pakistan – Every
month of the year a crop of wheat is harvested some
where in the world.
• In India extensively cultivated in North West India,
Eastern part, Central plain to some extent Southern
peninsular zone.
12Dr. Mamata K, Dept. of GPB, Chamarajanagara
13
Classification :
Important Species of Wheat
Dr. Mamata K, Dept. of GPB, Chamarajanagara
Classification :
14
Important Species of Wheat
Dr. Mamata K, Dept. of GPB, Chamarajanagara
Major cultivated species of wheat
• Durum - (T. durum) The only tetraploid form of wheat widely
used today, and the second most widely cultivated wheat today.
• Einkorn - (T. monococcum) A diploid species with wild and
cultivated variants . One of the earliest cultivated, but rarely
planted today.
• Common Wheat or Bread wheat - (T. aestivum) A hexaploid
species that is the most widely cultivated in the world.
• Emmer - (T. dicocum) A tetraploid species, cultivated in ancient
times but no longer in widespreaduse.
• Spelta - (T. spelta) Another hexaploid species cultivated in limited
quantities.
15Dr. Mamata K, Dept. of GPB, Chamarajanagara
16
DEVELOPMENT OF
COMMON WHEAT
The evolution of hexaploid bread wheat from its wild relatives.
17Dr. Mamata K, Dept. of GPB, Chamarajanagara
BREEDING OBJECTIVES
18Dr. Mamata K, Dept. of GPB, Chamarajanagara
19Dr. Mamata K, Dept. of GPB, Chamarajanagara
20Dr. Mamata K, Dept. of GPB, Chamarajanagara
Methods of breeding :
21Dr. Mamata K, Dept. of GPB, Chamarajanagara
•The CIMMYT, Mexico successfully utilized
the unique wheat germplasm, Norin 10 for
introducing dwarfing genes (Rht 1, Rht 2) for
increasing the yield potential and
resistance/tolerance against various biotic and
abiotic stresses in modem wheat varieties.
•Indian wheat scientists could develop large
number of improved wheat varieties from
wheat germplasm introduced from CIMMYT,
Mexico.
•Some of these varieties like Kalyansona,
Sonalika, C-306, WH 147, WL 711, HD
2329, WH 542 and PBW 343 have become
very popular among Indian farmers.
INTRODUCTION
22Dr. Mamata K, Dept. of GPB, Chamarajanagara
Cont..
• Theoretical considerations suggest
that wheat yield potential could be
increased by up to 50% through the
genetic improvement of radiation
use efficiency (RUE).
• Trait-based hybridization strategies
will aim to achieve their
simultaneous expression in elite
agronomic backgrounds, and wide
crossing will be employed to
augment genetic diversity where
needed.
• Genomic selection approaches will
be employed, especially for difficult-
to- phenotype traits.
23Dr. Mamata K, Dept. of GPB, Chamarajanagara
Pure Line Selection
In this method individual progenies are
evaluated and promising progenies are finally
selected old Indian tall varieties E.g. N-P-4, N-
P-6, N-P-12, PB-12, PB-11 were developed by
pure line selection.
24Dr. Mamata K, Dept. of GPB, Chamarajanagara
• The most common method used in self pollinated crops is pedigree
method of selection.
• The crosses are made between complimentary lines and records are
maintained of selections made over number of generations.
• The procedure provide selection opportunities generation after
generations.
• It allow breeder to identify bet combination with considerable
uniformity.
• The hybrid bulk selection method is relatively inexpensive, in which
generations are advanced without selection till F5 to F6 and much
material can be handled, nut often difficulty is isolation of superior
recombination.
• To overcome, this difficulty single decent method of selection is used
in which population remain constant over segregation generations.
• Varieties: a) Tilmely sown: NIAW-34, NIAW-301, NIAW-2496, HD-
2278, HD-2189.
b) Late Sown : HD-2501, Sonalika, HI-977, etc.
25
Pedigree Method
Dr. Mamata K, Dept. of GPB, Chamarajanagara
Back Cross Method
• This method is used when variety otherwise is good,
high yielding but deficient in simply inherited trait.
The obvious effect of this method the production
potential of improved variety is fixed at the level of
recurrent variety. Recently identified donors always
are used in back cross breeding programme.
• Stem Rust: Resistance gene- Sr2 From variety
Hope.
Leaf Rust: Resistance gene – Lr 13 from variety
Sonalika
26Dr. Mamata K, Dept. of GPB, Chamarajanagara
Multiline Breeding
• It is extension of back cross breeding and could be
called Multilateral backcrossing.
• It consist of spontaneous back cross programme to
produce isogenic lines for resistance to disease, in back
ground of some recurrent parent.
• Each isogenic line will be similar to recurrent parent
but they will differ for resistance to various
physiological farms of diseases.
• A mixture of these isogenic lines is called
multiline variety.
• E.g. ML-KS-11 (PAU, Ludhiana) and Bithoor
developed at CSAUAT, Kanpur.
27Dr. Mamata K, Dept. of GPB, Chamarajanagara
Mutation Breeding
• This method is used in depleted gene pool
situation. Chemical mutagenes EMS provide
broad spectrum genetic changes with lesser
sterility effects, as compared to X ray or
particular mutation.
• Varieties developed are 1) NP836, Sarbati
Sonora, Pusa larma, etc. are examples of
induced mutation and NP-11 is the examples
of spontaneous mutation.
28Dr. Mamata K, Dept. of GPB, Chamarajanagara
29Dr. Mamata K, Dept. of GPB, Chamarajanagara
30Dr. Mamata K, Dept. of GPB, Chamarajanagara
31Dr. Mamata K, Dept. of GPB, Chamarajanagara
32Dr. Mamata K, Dept. of GPB, Chamarajanagara
33Dr. Mamata K, Dept. of GPB, Chamarajanagara
34Dr. Mamata K, Dept. of GPB, Chamarajanagara
35Dr. Mamata K, Dept. of GPB, Chamarajanagara
36Dr. Mamata K, Dept. of GPB, Chamarajanagara
37Dr. Mamata K, Dept. of GPB, Chamarajanagara
38Dr. Mamata K, Dept. of GPB, Chamarajanagara
39Dr. Mamata K, Dept. of GPB, Chamarajanagara
Biotechnology
• In vitro production of haploids- Anther or pollen
culture is used to produce the haploid plants.
• The frequency of obtaining haploids increase when
anthers are treated with cold socks and heat
treatments.
• The haploids when treated with colchicines, the
homozygous diploid line can developed in short period
and can be used in hybrid breeding programme.
• The plantlets can be tested in vitro for different
stresses drought, salt, disease etc. to evaluate before
field screening.
40Dr. Mamata K, Dept. of GPB, Chamarajanagara
Speed breeding of wheat
Dr. Lee Hickey
• Technology first used by NASA to grow plants extra-
terrestrially is fast tracking improvements in a range
of crops.
• Speed breeding uses enhanced LED lighting and day-
long regimes of up to 22 hours to optimise
photosynthesis and promote rapid growth of crops.
It speeds up the breeding cycle of plants: for example,
six generations of wheat can be grown per year,
compared to two generations using traditional
breeding methods.
• By shortening breeding cycles, the method allows
scientists and plant breeders to fast-track genetic
improvements such as yield gain, disease resistance
and climate resilience in a range of crops such as
wheat, barley, oilseed rape and pea.
41Dr. Mamata K, Dept. of GPB, Chamarajanagara
Major breakthrough in deciphering bread wheat’s genetic
code
• In one of the largest genome projects ever
undertaken UK scientists have published a draft
sequence of the wheat genome in 2018.
• They identified about 96,000 genes and placed
them in an approximate order. Completing this
sequence represents a major achievement
because wheat has a very large and
complex genome.
• Bread wheat (Triticum aestivum) is a complex
hybrid, composed of the complete genomes of
three closely related grasses.
• It is like having tens of billions of Scrabble letters;
you know which letters are present, and their
quantities, but they need to be assembled on the
board in the right sequence before you can spell
out their order into genes’ Professor Neil Hall.
• The research will accelerate wheat improvement
by allowing wheat breeders and scientists to
identify useful genetic variation and select plants
can resists drought and disease.
Interesting fact: The
bread wheat genome is
five times larger than the
human genome and there
are three genomes in
each plant.
4542Dr. Mamata K, Dept. of GPB, Chamarajanagara
Rothamsted Wheat Trial: Second generation GM technology to
emulate natural plant defence mechanisms
• Currently a large proportion of UK wheat is treated
with broad spectrum chemical insecticides to
control cereal aphids.
• Unfortunately, repeated use of insecticides often
leads to resistant aphids and kills other non-target
insect species including the natural enemies of
aphids.
• Scientists from Rothamsted Research have
conducted a controlled experiment, combining
modern genetic engineering with their knowledge
of natural plant defences to test whether wheat
can repel aphid attack in the field.
• Their approach has been to use a natural odour, or
alarm pheromone, which aphids produce to alert
one another to danger. This odour also attracts the
natural enemies of aphids, e.g. ladybirds.
43Dr. Mamata K, Dept. of GPB, Chamarajanagara
44Dr. Mamata K, Dept. of GPB, Chamarajanagara
45Dr. Mamata K, Dept. of GPB, Chamarajanagara

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Breeding of wheat

  • 1. 1 Breeding of WHEAT Triticum aestivum (2n=6x=42) Dr. Mamata Khandappagol Assistant Professor (Contractual) Department of Genetics and Plant Breeding CoA, Chamarajanagara-571127 UAS, GKVK, Bengaluru Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 2. Introduction • Wheat is a staple crop for a significant proportion of the world’s population. • Wheat is rich in carbohydrates, protein and essential vitamins and minerals such as vitamins B and E, calcium and iron, as well as fibre. • It is world’s most widely cultured crop occupying 22% cultivated areas. • It is the major crop of USA, Canada and Asia, it is C3 crop not well adapted to tropical and subtropical condition. • As many as 25 species recognised in the world, only 3 species namely, T. aestivum/vulgare (Bread wheat), T. durum (Macroni wheat) & T. dicoccum (Emmer wheat) commercially grown in India. 2Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 3. History 3 • Cultivation of wheat began to spread beyond the Fertile Crescent after about 8000 BC • Spread of cultivated wheat started in the Fertile Crescent about 8500 BC, reaching Greece, Cyprus and India by 6500 BC, Egypt shortly after 6000 BC. • The early Egyptians were developers of bread and the use of the oven and developed baking into one of the first large-scale food production industries- Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 4. 4Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 5. 5 Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 6. 6 Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 7. Origin • Wheat has evolved from wild grasses. • The centre of origin is South Asia. • Large genetic variability is observed in Iran, Isreal, and Bordering countires. 7 Ploidy level Origin Diploid (2X) Asia minor Tetraploid (4X) Abyssinia, North Africa Hexaploid (6X) Central Asia Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 8. Region of wheat origin: Region of wheat cultivation: 8Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 9. Global Wheat Production 9 Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 10. 10Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 11. Wheat production in India 11Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 12. Distribution: • USA, Canada, Latin America, Europe, China, Japan, Argentina, Mexico, India, Pakistan – Every month of the year a crop of wheat is harvested some where in the world. • In India extensively cultivated in North West India, Eastern part, Central plain to some extent Southern peninsular zone. 12Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 13. 13 Classification : Important Species of Wheat Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 14. Classification : 14 Important Species of Wheat Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 15. Major cultivated species of wheat • Durum - (T. durum) The only tetraploid form of wheat widely used today, and the second most widely cultivated wheat today. • Einkorn - (T. monococcum) A diploid species with wild and cultivated variants . One of the earliest cultivated, but rarely planted today. • Common Wheat or Bread wheat - (T. aestivum) A hexaploid species that is the most widely cultivated in the world. • Emmer - (T. dicocum) A tetraploid species, cultivated in ancient times but no longer in widespreaduse. • Spelta - (T. spelta) Another hexaploid species cultivated in limited quantities. 15Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 17. The evolution of hexaploid bread wheat from its wild relatives. 17Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 18. BREEDING OBJECTIVES 18Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 19. 19Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 20. 20Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 21. Methods of breeding : 21Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 22. •The CIMMYT, Mexico successfully utilized the unique wheat germplasm, Norin 10 for introducing dwarfing genes (Rht 1, Rht 2) for increasing the yield potential and resistance/tolerance against various biotic and abiotic stresses in modem wheat varieties. •Indian wheat scientists could develop large number of improved wheat varieties from wheat germplasm introduced from CIMMYT, Mexico. •Some of these varieties like Kalyansona, Sonalika, C-306, WH 147, WL 711, HD 2329, WH 542 and PBW 343 have become very popular among Indian farmers. INTRODUCTION 22Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 23. Cont.. • Theoretical considerations suggest that wheat yield potential could be increased by up to 50% through the genetic improvement of radiation use efficiency (RUE). • Trait-based hybridization strategies will aim to achieve their simultaneous expression in elite agronomic backgrounds, and wide crossing will be employed to augment genetic diversity where needed. • Genomic selection approaches will be employed, especially for difficult- to- phenotype traits. 23Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 24. Pure Line Selection In this method individual progenies are evaluated and promising progenies are finally selected old Indian tall varieties E.g. N-P-4, N- P-6, N-P-12, PB-12, PB-11 were developed by pure line selection. 24Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 25. • The most common method used in self pollinated crops is pedigree method of selection. • The crosses are made between complimentary lines and records are maintained of selections made over number of generations. • The procedure provide selection opportunities generation after generations. • It allow breeder to identify bet combination with considerable uniformity. • The hybrid bulk selection method is relatively inexpensive, in which generations are advanced without selection till F5 to F6 and much material can be handled, nut often difficulty is isolation of superior recombination. • To overcome, this difficulty single decent method of selection is used in which population remain constant over segregation generations. • Varieties: a) Tilmely sown: NIAW-34, NIAW-301, NIAW-2496, HD- 2278, HD-2189. b) Late Sown : HD-2501, Sonalika, HI-977, etc. 25 Pedigree Method Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 26. Back Cross Method • This method is used when variety otherwise is good, high yielding but deficient in simply inherited trait. The obvious effect of this method the production potential of improved variety is fixed at the level of recurrent variety. Recently identified donors always are used in back cross breeding programme. • Stem Rust: Resistance gene- Sr2 From variety Hope. Leaf Rust: Resistance gene – Lr 13 from variety Sonalika 26Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 27. Multiline Breeding • It is extension of back cross breeding and could be called Multilateral backcrossing. • It consist of spontaneous back cross programme to produce isogenic lines for resistance to disease, in back ground of some recurrent parent. • Each isogenic line will be similar to recurrent parent but they will differ for resistance to various physiological farms of diseases. • A mixture of these isogenic lines is called multiline variety. • E.g. ML-KS-11 (PAU, Ludhiana) and Bithoor developed at CSAUAT, Kanpur. 27Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 28. Mutation Breeding • This method is used in depleted gene pool situation. Chemical mutagenes EMS provide broad spectrum genetic changes with lesser sterility effects, as compared to X ray or particular mutation. • Varieties developed are 1) NP836, Sarbati Sonora, Pusa larma, etc. are examples of induced mutation and NP-11 is the examples of spontaneous mutation. 28Dr. Mamata K, Dept. of GPB, Chamarajanagara
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  • 39. 39Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 40. Biotechnology • In vitro production of haploids- Anther or pollen culture is used to produce the haploid plants. • The frequency of obtaining haploids increase when anthers are treated with cold socks and heat treatments. • The haploids when treated with colchicines, the homozygous diploid line can developed in short period and can be used in hybrid breeding programme. • The plantlets can be tested in vitro for different stresses drought, salt, disease etc. to evaluate before field screening. 40Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 41. Speed breeding of wheat Dr. Lee Hickey • Technology first used by NASA to grow plants extra- terrestrially is fast tracking improvements in a range of crops. • Speed breeding uses enhanced LED lighting and day- long regimes of up to 22 hours to optimise photosynthesis and promote rapid growth of crops. It speeds up the breeding cycle of plants: for example, six generations of wheat can be grown per year, compared to two generations using traditional breeding methods. • By shortening breeding cycles, the method allows scientists and plant breeders to fast-track genetic improvements such as yield gain, disease resistance and climate resilience in a range of crops such as wheat, barley, oilseed rape and pea. 41Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 42. Major breakthrough in deciphering bread wheat’s genetic code • In one of the largest genome projects ever undertaken UK scientists have published a draft sequence of the wheat genome in 2018. • They identified about 96,000 genes and placed them in an approximate order. Completing this sequence represents a major achievement because wheat has a very large and complex genome. • Bread wheat (Triticum aestivum) is a complex hybrid, composed of the complete genomes of three closely related grasses. • It is like having tens of billions of Scrabble letters; you know which letters are present, and their quantities, but they need to be assembled on the board in the right sequence before you can spell out their order into genes’ Professor Neil Hall. • The research will accelerate wheat improvement by allowing wheat breeders and scientists to identify useful genetic variation and select plants can resists drought and disease. Interesting fact: The bread wheat genome is five times larger than the human genome and there are three genomes in each plant. 4542Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 43. Rothamsted Wheat Trial: Second generation GM technology to emulate natural plant defence mechanisms • Currently a large proportion of UK wheat is treated with broad spectrum chemical insecticides to control cereal aphids. • Unfortunately, repeated use of insecticides often leads to resistant aphids and kills other non-target insect species including the natural enemies of aphids. • Scientists from Rothamsted Research have conducted a controlled experiment, combining modern genetic engineering with their knowledge of natural plant defences to test whether wheat can repel aphid attack in the field. • Their approach has been to use a natural odour, or alarm pheromone, which aphids produce to alert one another to danger. This odour also attracts the natural enemies of aphids, e.g. ladybirds. 43Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 44. 44Dr. Mamata K, Dept. of GPB, Chamarajanagara
  • 45. 45Dr. Mamata K, Dept. of GPB, Chamarajanagara