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BIOENERGETICS
GENERAL BIOLOGY 1
Prepared by: Ms. Louie Jane T. Eleccion
Transformation of Energy in Living Organisms
ENERGY
•Needed to perform heavy labor and exercise.
•It is required for both the synthesis and
breakdown of molecules.
Process Questions:
•From where, and in what form, does this energy
come?
•How do living cells obtain energy, and how do they
use it?
ELECTRONS AND
ENERGY
Redox Reactions
OXIDATION-REDUCTION (REDOX)
•Oxidation – removal of an electron in a
compound.
•Reduction – addition of this electron to
another compound.
Redox Reactions
•Removal of an electron from a molecule, results in
decreased potential energy, and its addition increases the
potential energy of the other.
•Most of the energy stored in atoms and used to fuel cell
function is in the form of high-energy electrons.
•The transfer of energy in the form of electrons allows the
cell to transfer and use energy in an incremental fashion.
ELECTRON CARRIERS
•In living systems, a small class of compounds
functions as electrons.
•They bind and carry high-energy electrons
between compounds in pathways.
•They are derived from the B vitamin group and
are derivatives of nucleotide.
ATP: ADENOSINE
TRIPHOSPHATE
The Energy Currency of the Cell
•A living cell cannot store significant amounts of energy
•Excess energy would result in an excessive thermal
condition that could damage and destroy the cell.
•A cell must be able to handle that energy in a way that
enables the cell to store energy and release it for use
only as needed.
•When ATP is broken down, usually by removal of its
terminal phosphate group, energy is released—which
energy is used by our cells to do work. Usually by the
released phosphate binds to another molecule,
activating it.
•Phosphoanhydride bonds – between phosphate
groups of ATP.
ENERGY FROM ATP
•Hydrolysis – water is split, resulting hydrogen atom
and hydroxyl group.
•The hydrolysis of ATP produces ADP (Adenosine
diphosphate), with an inorganic phosphate ion (Pi)
and the release of free energy. Hydrolysis is
reversible!
•To carry out life processes, ATP is continuously
broken down into ADP.
•ADP is continuously regenerated into ATP by the
reattachment of a third phosphate group.
ENERGY FROM ATP
Where does this energy come from?
•In nearly every living thing on earth, the energy
comes from the metabolism of glucose.
•ATP is a direct link between the limited set of
exergonic pathways of glucose catabolism and the
multitude of endergonic pathways that power living
cells.
METABOLISM
Sum of all the chemical activities taking place in an organism
Catabolism
•Larger molecules broken down into smaller ones
--Stages 1-4 (Digestion; Formation of Acetyl CoA;
Citric Acid Cycle; Electron Transport Chain &
Oxidative Phosphorylation)
•Releases energy (may be stored temporarily as ATP)
•Ex. CELLULAR RESPIRATION
•Acetyl-CoA (acetyl coenzyme A) is a molecule that
participates in many biochemical reactions in
carbohydrate and lipid metabolism.
•Its main function is to deliver the acetyl group to the
citric acid cycle (Krebs cycle) to be oxidized for
production.
Anabolism
•Complex molecules synthesized from simpler
substances
•Absorbs energy & stores it as chemical bonds
•Ex. PHOTOSYNTHESIS
PHOTOSYNTHESIS
Catabolic Pathway
Introduction
•From the Greek…
PHOTO = produced by light
SYNTHESIS = a whole made of parts put together.
Definition: PHOTOSYNTHESIS is the process whereby plants, algae,
some bacteria, use the energy of the sun to synthesize organic
compounds (sugars) from inorganic compounds (CO2 and water).
Importance
PHOTOSYNTHESIS is one of the most important
biological process on earth!
• Provides the oxygen we breathe
• Consumes much of the CO2
• Food
• Energy
2 Processes of Photosynthesis
• Light-dependent Reaction
-Energy from sunlight is absorbed by chlorophyll and that energy is
converted into stored chemical energy.
-Occurs in the thylakoid lumen.
• Light-independent Reactions (Calvin Cycle)
- Chemical energy harvested during the light-dependent reactions drive
the assembly of sugar molecules from carbon dioxide.
- Occurs in the chloroplast stroma.
General Formula
light
6 CO2 + 12 H2O ---------> C6H12O6 + 6 O2 + 6 H2O
pigments, enzymes
LIGHT-DEPENDENT REACTION
•Requires only
water and light
to start the
Process.
•Produce NADPH
and ATP as its final
products
LIGHT-INDEPENDENT REACTION
• Requires one molecule
of carbon dioxide to
initiate the process.
• Produce glucose
(C6 H12 O6) as its
final product.
WATCH THE VIDEOS ABOUT
PHOTOSYNTHESIS AND CALVIN CYCLE
on our facebook page!
CELLULAR
RESPIRATION
Anabolic Pathway
•Cellular respiration starts with OXYGEN and
GLUCOSE…
•Can make up to 38 molecules of ATP!
3 STAGES:
•GLYCOLYSIS – breaking down of glucose
•THE KREB’S CYCLE - produces NADH and
FADH2 for ETC and fuels production of ATP.
•ELECTRON TRANSPORT CHAIN
GLYCOLYSIS (Summary)
• Anaerobic process (requires NO OXYGEN)
• Occurs in the cell’s cytoplasm.
• Invests 2 molecules of ATP in order to work (split glucose into half).
• Energy return of 4 ATP molecules.
• But the ATP Net Yield is only 2 since the process invests 2 ATP
molecules at the beginning of glycolysis.
• Produces 2 NADH molecules (which will enter ETC).
• Also produces 2 pyruvate molecules/pyruvic acid (which will enter The
Kreb’s Cycle).
THE KREB’S CYCLE (Summary)
• Aerobic process (Requires Oxygen)
• Occurs in the mitochondrial matrix of the cell.
• Needs the pyruvate from glycolysis to start the process, together with
Acetyl Co-enzyme A.
• Two CO2 and 2 NADH will be released –prep stage.
• Acetyl Co-A combines with Oxaloacetate to make citrate/citric acid.
• Uses FAD+ electron carrier which is reduced to FADH+
• Produce 4 CO2, 6 NADH, 2 FADH2, 2 ATP
ELECTRON TRANSPORT CHAIN
• Aerobic process (Requires Oxygen!)
• Occurs in the mitochondrial inner folding (cristae).
• Uses four complexes to transport electrons creating proton
gradient (H+) to generate the function of ATP Synthase.
• Requires NADH and FADH2 molecules to generate 34 ATPs.
Don't let your teacher affect your love for science. You
can be a scientist outside of the classroom too. Be
your own teacher. Keep your love of learning and you
will do just fine.
ENJOY THE VIDEOS! 

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BIOENERGETICS

  • 1. BIOENERGETICS GENERAL BIOLOGY 1 Prepared by: Ms. Louie Jane T. Eleccion Transformation of Energy in Living Organisms
  • 2.
  • 3. ENERGY •Needed to perform heavy labor and exercise. •It is required for both the synthesis and breakdown of molecules.
  • 4. Process Questions: •From where, and in what form, does this energy come? •How do living cells obtain energy, and how do they use it?
  • 6. OXIDATION-REDUCTION (REDOX) •Oxidation – removal of an electron in a compound. •Reduction – addition of this electron to another compound.
  • 7.
  • 8. Redox Reactions •Removal of an electron from a molecule, results in decreased potential energy, and its addition increases the potential energy of the other. •Most of the energy stored in atoms and used to fuel cell function is in the form of high-energy electrons. •The transfer of energy in the form of electrons allows the cell to transfer and use energy in an incremental fashion.
  • 9. ELECTRON CARRIERS •In living systems, a small class of compounds functions as electrons. •They bind and carry high-energy electrons between compounds in pathways. •They are derived from the B vitamin group and are derivatives of nucleotide.
  • 10.
  • 11.
  • 12.
  • 13.
  • 15. •A living cell cannot store significant amounts of energy •Excess energy would result in an excessive thermal condition that could damage and destroy the cell. •A cell must be able to handle that energy in a way that enables the cell to store energy and release it for use only as needed.
  • 16.
  • 17. •When ATP is broken down, usually by removal of its terminal phosphate group, energy is released—which energy is used by our cells to do work. Usually by the released phosphate binds to another molecule, activating it. •Phosphoanhydride bonds – between phosphate groups of ATP.
  • 18.
  • 20. •Hydrolysis – water is split, resulting hydrogen atom and hydroxyl group. •The hydrolysis of ATP produces ADP (Adenosine diphosphate), with an inorganic phosphate ion (Pi) and the release of free energy. Hydrolysis is reversible!
  • 21. •To carry out life processes, ATP is continuously broken down into ADP. •ADP is continuously regenerated into ATP by the reattachment of a third phosphate group. ENERGY FROM ATP
  • 22. Where does this energy come from? •In nearly every living thing on earth, the energy comes from the metabolism of glucose. •ATP is a direct link between the limited set of exergonic pathways of glucose catabolism and the multitude of endergonic pathways that power living cells.
  • 23. METABOLISM Sum of all the chemical activities taking place in an organism
  • 24. Catabolism •Larger molecules broken down into smaller ones --Stages 1-4 (Digestion; Formation of Acetyl CoA; Citric Acid Cycle; Electron Transport Chain & Oxidative Phosphorylation) •Releases energy (may be stored temporarily as ATP) •Ex. CELLULAR RESPIRATION
  • 25. •Acetyl-CoA (acetyl coenzyme A) is a molecule that participates in many biochemical reactions in carbohydrate and lipid metabolism. •Its main function is to deliver the acetyl group to the citric acid cycle (Krebs cycle) to be oxidized for production.
  • 26. Anabolism •Complex molecules synthesized from simpler substances •Absorbs energy & stores it as chemical bonds •Ex. PHOTOSYNTHESIS
  • 28. Introduction •From the Greek… PHOTO = produced by light SYNTHESIS = a whole made of parts put together. Definition: PHOTOSYNTHESIS is the process whereby plants, algae, some bacteria, use the energy of the sun to synthesize organic compounds (sugars) from inorganic compounds (CO2 and water).
  • 29. Importance PHOTOSYNTHESIS is one of the most important biological process on earth! • Provides the oxygen we breathe • Consumes much of the CO2 • Food • Energy
  • 30. 2 Processes of Photosynthesis • Light-dependent Reaction -Energy from sunlight is absorbed by chlorophyll and that energy is converted into stored chemical energy. -Occurs in the thylakoid lumen. • Light-independent Reactions (Calvin Cycle) - Chemical energy harvested during the light-dependent reactions drive the assembly of sugar molecules from carbon dioxide. - Occurs in the chloroplast stroma.
  • 31. General Formula light 6 CO2 + 12 H2O ---------> C6H12O6 + 6 O2 + 6 H2O pigments, enzymes
  • 32. LIGHT-DEPENDENT REACTION •Requires only water and light to start the Process. •Produce NADPH and ATP as its final products
  • 33. LIGHT-INDEPENDENT REACTION • Requires one molecule of carbon dioxide to initiate the process. • Produce glucose (C6 H12 O6) as its final product.
  • 34.
  • 35. WATCH THE VIDEOS ABOUT PHOTOSYNTHESIS AND CALVIN CYCLE on our facebook page!
  • 37. •Cellular respiration starts with OXYGEN and GLUCOSE… •Can make up to 38 molecules of ATP!
  • 38. 3 STAGES: •GLYCOLYSIS – breaking down of glucose •THE KREB’S CYCLE - produces NADH and FADH2 for ETC and fuels production of ATP. •ELECTRON TRANSPORT CHAIN
  • 39.
  • 40. GLYCOLYSIS (Summary) • Anaerobic process (requires NO OXYGEN) • Occurs in the cell’s cytoplasm. • Invests 2 molecules of ATP in order to work (split glucose into half). • Energy return of 4 ATP molecules. • But the ATP Net Yield is only 2 since the process invests 2 ATP molecules at the beginning of glycolysis. • Produces 2 NADH molecules (which will enter ETC). • Also produces 2 pyruvate molecules/pyruvic acid (which will enter The Kreb’s Cycle).
  • 41.
  • 42. THE KREB’S CYCLE (Summary) • Aerobic process (Requires Oxygen) • Occurs in the mitochondrial matrix of the cell. • Needs the pyruvate from glycolysis to start the process, together with Acetyl Co-enzyme A. • Two CO2 and 2 NADH will be released –prep stage. • Acetyl Co-A combines with Oxaloacetate to make citrate/citric acid. • Uses FAD+ electron carrier which is reduced to FADH+ • Produce 4 CO2, 6 NADH, 2 FADH2, 2 ATP
  • 43.
  • 44. ELECTRON TRANSPORT CHAIN • Aerobic process (Requires Oxygen!) • Occurs in the mitochondrial inner folding (cristae). • Uses four complexes to transport electrons creating proton gradient (H+) to generate the function of ATP Synthase. • Requires NADH and FADH2 molecules to generate 34 ATPs.
  • 45.
  • 46. Don't let your teacher affect your love for science. You can be a scientist outside of the classroom too. Be your own teacher. Keep your love of learning and you will do just fine. ENJOY THE VIDEOS! 

Editor's Notes

  1. This involves cellular processes such as the building and breaking down of complex molecules occur through stepwise chemical reactions. Some of these chemical reactions are spontaneous and release energy, whereas other requires energy to proceed.
  2. We use great deal of energy while thinking and even during sleeping. In fact, the living cells constantly use energy.
  3. Energy production within a cell involves many coordinated chemical pathways and most of these pathways are combinations of OXIDATION and REDUCTION.
  4. Since they occur together, these pairs of electrons are called Redox Reactions.
  5. There are eight types of vitamin B: thiamin (B1), riboflavin, niacin, pantothenic acid, biotin, vitamin B6 (pyridoxine), folate (called folic acid when included in supplements), vitamin B12 (cyanocobalamin). Get all eight B vitamins ​from a variety of foods: Whole grains (brown rice, barley, millet), Meat (red meat, poultry, fish), Eggs and dairy products (milk, cheese), Legumes (beans, lentils), Seeds and nuts (sunflower seeds, almonds), Dark, leafy vegetables (broccoli, spinach, kai lan)
  6. These are the compounds that can be easily reduced (they accept electrons) or oxidized (they lose electrons). NAD (Nicotinamide Adenine Dinucleotide) – derived from vitamin B, Niacin (lowers cholesterol, ease arthritis and boost brain function) NAD+ (Oxidized from) – can accept electrons from an organic molecule; NADH (Reduced form)
  7. Another variation of NAD, NADP, contains an extra phosphate group.
  8. Similarly, FAD is derived from vitamin B2. Reduced form is FADH2.
  9. NAD+ (Oxidized from) – can accept electrons from an organic molecule; NADH (Reduced form) Both of NAD+ and FAD+ are extensively used in energy extraction from sugars (Cellular Respiration). And NADP plays an important role in anabolic reactions and photosynthesis.
  10. ATP is often called the energy currency of the cell.
  11. An ATP functions like that of a rechargeable battery.
  12. ATP alters the structure of integral proteins, changing its affinity for sodium and potassium. In this way, the cell performs work, pumping ions against their electrochemical gradients.
  13. Oxygen on earth allowed for the evolution of aerobic respiration and higher life-forms.