This document summarizes several laboratory tests used to identify carbohydrates and proteins. For carbohydrates, it describes tests such as Molisch's test, iodine test, Benedict's test, Barfoed's test, Seliwanoff's test, and osazone test. It explains the procedures and mechanisms for how each test identifies different types of carbohydrates. For proteins, it lists common color reactions used to detect specific amino acids or protein components, such as the biuret test, ninhydrin test, xanthoproteic test, and others. It provides the reactions and mechanisms for how these color tests can identify proteins and their constituents.
A brief presentation for second-year students in Iraqi Technical Institutes (studying Medical Laboratory Technology). This introduction covers also the teaching laboratories.
Pathological analysis of body fluids with lab investigations,
Including Amniotic fluid, Semen analysis, Synovial fluid, Gastric fluid
Other body fluids: Sweat,saliva,tear
A brief presentation for second-year students in Iraqi Technical Institutes (studying Medical Laboratory Technology). This introduction covers also the teaching laboratories.
Pathological analysis of body fluids with lab investigations,
Including Amniotic fluid, Semen analysis, Synovial fluid, Gastric fluid
Other body fluids: Sweat,saliva,tear
Biochemistry is a basic science which deals with chemical nature and chemical behaviour of living matter and with the reactions and processes they undergo.
Biochemistry involves the study of:
Chemical constituents of living matter.
Chemical changes which occur in the organism during digestion, absorption and excretion.
Chemical changes which occur during growth and multiplication of the organism.
Transformation of one form of chemical constituent to the other.
Energy changes involved in such transformation.
Note:- The term “Biochemistry” was first introduced by German chemist Carl Neuberg in 1903 from Greek word “bios” means “life”.
It is mainly deals with the biochemical aspects that are involved in several conditions.
The results of qualitative and quantitative analysis of body fluids assist the clinicians in the diagnosis, treatment and prevention of the disease and drug monitoring, tissue and organ transplantation, forensic investigations and so on.
Various biological fluids subjected to chemical tests and assays include blood, plasma, serum, urine, cerebrospinal fluid (CSF), ascetic fluid, pleural fluid, faeces, calculi and tissues.
Note:- Modern day medical practice is highly dependent on the laboratory analysis of body fluids, especially the blood. The disease manifestations are reflected in the composition of blood and other tissues.
Hence, the demarcation of abnormal from normal constituents of the body is another aim of the study of clinical biochemistry.
Basics of DNA isolation, What is chemistry behind it. Presently the laboratory of animal science department ,Göttingen university using this technique for dna isolation in pig blood sample.
Biochemistry is a basic science which deals with chemical nature and chemical behaviour of living matter and with the reactions and processes they undergo.
Biochemistry involves the study of:
Chemical constituents of living matter.
Chemical changes which occur in the organism during digestion, absorption and excretion.
Chemical changes which occur during growth and multiplication of the organism.
Transformation of one form of chemical constituent to the other.
Energy changes involved in such transformation.
Note:- The term “Biochemistry” was first introduced by German chemist Carl Neuberg in 1903 from Greek word “bios” means “life”.
It is mainly deals with the biochemical aspects that are involved in several conditions.
The results of qualitative and quantitative analysis of body fluids assist the clinicians in the diagnosis, treatment and prevention of the disease and drug monitoring, tissue and organ transplantation, forensic investigations and so on.
Various biological fluids subjected to chemical tests and assays include blood, plasma, serum, urine, cerebrospinal fluid (CSF), ascetic fluid, pleural fluid, faeces, calculi and tissues.
Note:- Modern day medical practice is highly dependent on the laboratory analysis of body fluids, especially the blood. The disease manifestations are reflected in the composition of blood and other tissues.
Hence, the demarcation of abnormal from normal constituents of the body is another aim of the study of clinical biochemistry.
Basics of DNA isolation, What is chemistry behind it. Presently the laboratory of animal science department ,Göttingen university using this technique for dna isolation in pig blood sample.
Based on the reactivity with Tollen’s, Benedict’s or Fehling’s reagent, carbohydrates are classified as;
Reducing sugars
Carbohydrates that can reduce Tollen’s, Benedict’s or Fehling’s reagents are called reducing sugars (sugar with free aldehyde or ketone group). All monosaccharides and most of the disaccharides are reducing sugars. Some examples are Maltose and Lactose.
Non-reducing sugars
Carbohydrates that cannot reduce Tollen’s, Benedict’s or Fehling’s reagents are called non-reducing sugars. Sucrose is a non-reducing sugar.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
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These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
2. CARBOHYDRATE
1. Molisch’s Test
A)procedure:-1 ml of carbohydrate soln + 2-3 drops of α-napthol + 2 ml
conc. H2so4
B)Observation:-Violet ring at the junction of the two liquids
C)Mechanism:-
It is a general test for the detection of carbohydrates. The strong H2so4
Hydrolyses carbohydrate (poly- and disaccharides) to liberate monosac-
Charides. The monosaccharide get dehydrated to form furfural (from pe-
Toses) or hydroxy methylfurfural (from hexoses) which condense with
α-naphthol to form a violet coloured complex.
3. IODINE TEST
1)Procedure:- 2ml of carbohydrate soln + few drops of iodine soln
Deep blue color:- with starch(non-reducing polysaccharides)
Purple color:-Dextrin soln
2)Mechanism:-
Starch>soluble starch>Amylodextrin(purple)>Erythrodextrin(red)>
Arcrodextrin(No colour)> maltose( end product of enzymatic hydrolysis of
strach)
Polysaccharides combine with iodine
to form a coloured complex.
-ve proceeds for benedict’s test
+ve proceeds for acid hydrolysis
4. Benedict’s test
>5 ml Bendict’s reagent+ 1ml of carbohydrate soln>heat for 1-2 minutes
>semiquantative quantative test because cuprous oxide gives different
colour with different concn of carbohydrate soln.
0.5-1%>>>>>>Green
1-1.5%>>>>>>>>yellow
1.5-2%>>>>>>>>>orange
2-2.5%>>>>>>>>>>>Red
composition of Benedict’s reagent:- copper sulphate (BLUE SOLN),
Sodium citrate(prevents cuprous ion)
Sodium carbonate (mild alkali)
Mechanism :- This is a test for the identification of reducing sugars,
which form enediols (predominantly under alkaline conditions). The
enediol forrms of sugars reduce cupric ions (cu++) of copper sulfate to
coprous ions(cu+) which form a yellow precipiate of cuprous hydroxide or a
brick red precipatate of cuprous oxide.
5. Barfoed’s Test
Procedure:- 1ml of carbohydrate soln + 1ml of Barfoed’s soln
Composition of Barfoed’s reagent
<<<Cupric acetate
<<< Acetic Acid
MECHANISM:-
The principle of this test is the same as that of a Benedict’s test except that
the reduction is carried in mild acidic medium. Since acidic medium is not
favorable for reduction, only strong reducing sugars (monosaccharide)
give this test positive. Thus, Barfoed’s test serves as a key reaction to
distingush monosaccharide form disaccharides.
Observation :- Scanty re d ppt at the bottom of the test tube.
6. Seliwanoff’s test
CLINICAL SIGNIFICANCE :-
Thrombosis ,Degeneration, Necrosis
Composition of Seliwanoff’s reagent:-
Resor cinol(ALDEHYDE COMPOUND)
HCL(Dehydrates Ketohexose more rapidally to form furfural
derivatives)
Fructose-+VE
Glucose—VE
Procedure:-1ml of carbohydrate soln+ 3ml of Seliwanoff reagent heat for
30 sec.
MECHANISM:- This is a specific test for ketohexoses.
Concentrated HCL dehydrates Ketohexoses to form furfural derivatives
which condense with resorcinol to give a cherry red complex.
7. OSAZONE TEST
Procedure:- 2ml of sugar soln+2ml of osazone reagent mix and heat over
boiling water bath.
Osazone reagent :- 3 molecules of phenyl hyadrazine
Osazone –yellow crystals, crystalline derivatives of
sugar and performed only with reducing
sugar
Mechanism:-Phenylhydrazine in acetic acid, when boiled
with reducing sugars forms osazones. The first two carbons(c1
and c2) are involved in this rxn. The sugars that differ in their
configuration on these two carbons give the same type of
osazones, since the difference is marked by binding with
phenyl hydrazine. Thus, glucose, fructose, and mannose give
the same type (needle shaped), maltose- sunflower shaped
while lacose gives powder puff shaped
8. Sucrose hydrolysis Test
Procedure:-2ml of sucrose solution in test tube+ 5 drops of conc. Hcl boil
for 1 min over small flame and cool the contents and add 40% NaOH soln.
Mechanism:- Sucrose is a non- reducing sugar. Hence, it does not give
Benedict’s and Barfoed’s tests. Sucrose can be hydrolyzed by conc. HCL, to be con
verted to converted to glucose and fructose which answer the reducing rxns.
However, after sucrose hydrolysis, the medium has to be made alkaline (by adding
40% NaOH) for effective reduction process.
10. Color rxn of protein
Reaction Specific group or amino
acid
Biuret Two peptide linkages
Ninhydrin rxn Alfa-amino acids
Xanthoproteic rxn Aromatic amino acid(phe,
Tyr, Trp)
Million’s rxn Phenolic group(tyr)
Hopkins’s rxn(Aldehyde test) Indole ring (Trp)
Sakaguchi’s test Guanidino group(Arg)
Sulphur test Sulfhydryl group(Cystine &
Cystiene)
Pauly’s test Imidazole ring(His)
Molisch’s test Carbohydrate moiety
Test for organic phosphate Casein
11. Reaction Mechanism
Biuret In alkaline medium, peptide bond
+cupric ion to form violet colored
complex
Ninhydrin rxn 1)AA + ninhydrinketo-acid+
NH3+Co2+Hydrinadantin
2) Hydrinadantin+NH3+Ninhydrin
Ruhemann’s purple
Xanthoproteic rxn (Nitratation rxn)
Phenyl gr. + HNO3nitrophenyl gr.(yellow)
While adding alkali to the nitrophenyl soln
gives orange colour
Million’s rxn Phenolic gr.
+Hgso4mercurictyrosine complex
which on nitration give red color with
sod. nitrate
Hopkins’s rxn(Aldehyde test) INDOLE gr.+Formaldehyde+(in presence of
oxidizing agent H2SO4 with mercuric
sulphate)violet colored complex
Sakaguchi’s test In alkaline medm(40% NaOH), α-
napathol+Guanidino Gr.complex
which is oxidized by sod. Hypobromide
to give red colour complex
12. Sulphur test When cysteine and cystine are
boiled with NaOH, organic sulfur is
converted to inorganic sodium
sulfide. This reacts with lead
aceatate to form black ppt. of lead
sulfide. Methionine doesnot give this
test, since sulfur of methionine isnot
split by alkali
Pauly’s test Diazotised sulfanilic acid
reacts with imidazole ring om
alkaline medium to form a red
colored complex.
Molish’s test The proteins containing
carbohydrate(Glycoprotein )
give +ve to this test.
(ALBUMIN)
Test for organic
phosphate