Viruses are small, acellular particles that can replicate only in a host cell. They are obligatory intracellular parasites.They
consist of a nucleic acid genome enclosed in a protective protein shell or capsidBacteriophage is the virus that infect bacteria.Bacteriophages were discovered by Frederick Twort(1915)and Felix d'Herelle(1917).
A bacteriophage (informally, phage) is a virus that infects and replicates within a bacterium. The term is derived from "bacteria" and the Greek (phagein), "to devour". Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome, and may have relatively simple or elaborate structures. Their genomes may encode as few as four genes, and as many as hundreds of genes. Phages replicate within the bacterium following the injection of their genome into its cytoplasm. Bacteriophages are among the most common and diverse entities in the biosphere.
Phages are widely distributed in locations populated by bacterial hosts, such as soil or the intestines of animals. One of the densest natural sources for phages and other viruses is sea water, where up to 9×108 virions per milliliter have been found in microbial mats at the surface,] and up to 70% of marine bacteria may be infected by phages. They have been used for over 90 years as an alternative to antibiotics in the former Soviet Union and Central Europe, as well as in France. They are seen as a possible therapy against multi-drug-resistant strains of many bacteria (see phage therapy). Nevertheless, phages of Inoviridae have been shown to complicate biofilms involved in pneumonia and cystic fibrosis, shelter the bacteria from drugs meant to eradicate disease and promote persistent infection
Viruses that infect and parsitized bacteria is known as bacteriophage.
It was discovered by Frederick.W.Twort in Great Britian (1915) and Felix d’ Herelle in France(1917).
D’ Herelle coined the term bacteriophage meaning ‘bacterial eater’ to describe the agent’s bacteriocidal activity. He observed lysis of a broth culture of a dysentry bacillus.
A bacteriophage (informally, phage) is a virus that infects and replicates within a bacterium. The term is derived from "bacteria" and the Greek (phagein), "to devour". Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome, and may have relatively simple or elaborate structures. Their genomes may encode as few as four genes, and as many as hundreds of genes. Phages replicate within the bacterium following the injection of their genome into its cytoplasm. Bacteriophages are among the most common and diverse entities in the biosphere.
Phages are widely distributed in locations populated by bacterial hosts, such as soil or the intestines of animals. One of the densest natural sources for phages and other viruses is sea water, where up to 9×108 virions per milliliter have been found in microbial mats at the surface,] and up to 70% of marine bacteria may be infected by phages. They have been used for over 90 years as an alternative to antibiotics in the former Soviet Union and Central Europe, as well as in France. They are seen as a possible therapy against multi-drug-resistant strains of many bacteria (see phage therapy). Nevertheless, phages of Inoviridae have been shown to complicate biofilms involved in pneumonia and cystic fibrosis, shelter the bacteria from drugs meant to eradicate disease and promote persistent infection
Viruses that infect and parsitized bacteria is known as bacteriophage.
It was discovered by Frederick.W.Twort in Great Britian (1915) and Felix d’ Herelle in France(1917).
D’ Herelle coined the term bacteriophage meaning ‘bacterial eater’ to describe the agent’s bacteriocidal activity. He observed lysis of a broth culture of a dysentry bacillus.
TOBACCO MOSAIC VIRUS (Genome organization &their replication) TMV is a plant virus which infects a wide range of plants, especially tobacco and other members of the family Solanaceae and cucumbers, and a number of ornamental flowers.
Bergey's Manual and it's classification. A brief concised presentation prepared for taking seminar and classes.
Volume II (Edition 2) described more in detail.
TOBACCO MOSAIC VIRUS (Genome organization &their replication) TMV is a plant virus which infects a wide range of plants, especially tobacco and other members of the family Solanaceae and cucumbers, and a number of ornamental flowers.
Bergey's Manual and it's classification. A brief concised presentation prepared for taking seminar and classes.
Volume II (Edition 2) described more in detail.
Bacteriophage introduction,Discovery of Phage,classification,Structure of Bacteriophage,Morphological Groups and Life Cycle of Bacteriophage and how it's attack on bacteria.
or bacteriophages as they are known (phage for short, from Gr..docxgerardkortney
or "bacteriophages" as they are known ("phage" for short, from Gr. "phagein" meaning "to eat" or "to nibble"). Most research has been done on the phages that attack E. coli, especially the T-phages and phage lambda.
Like most viruses, bacteriophages typically carry only the genetic information needed for replication of their nucleic acid and synthesis of their protein coats. When phages infect their host cell, the order of business is to replicate their nucleic acid and to produce the protective protein coat. But they cannot do this alone. They require precursors, energy generation and ribosomes supplied by their bacterial host cell.
Bacterial cells can undergo one of two types of infections by viruses termed lytic infections andlysogenic (temperate) infections. In E. coli, lytic infections are caused by a group seven phages known as the T-phages, while lysogenic infections are caused by the phage lambda.
Lytic Infections
The T-phages, T1 through T7, are referred to as lytic phages because they always bring about the lysis and death of their host cell, the bacterium E. coli. T-phages contain double-stranded DNA as their genetic material. In addition to their protein coat or capsid (also referred to as the "head"), T-phages also possess a tail and some related structures. A diagram and electron micrograph of bacteriophage T4 is shown below. The tail includes a core, a tail sheath, base plate, tail pins, and tail fibers, all of which are composed of different proteins. The tail and related structures of bacteriophages are generally involved in attachment of the phage and securing the entry of the viral nucleic acid into the host cell.
or "bacteriophages" as they are known ("phage" for short, from Gr. "phagein" meaning "to eat" or "to nibble"). Most research has been done on the phages that attack E. coli, especially the T-phages and phage lambda.
Like most viruses, bacteriophages typically carry only the genetic information needed for replication of their nucleic acid and synthesis of their protein coats. When phages infect their host cell, the order of business is to replicate their nucleic acid and to produce the protective protein coat. But they cannot do this alone. They require precursors, energy generation and ribosomes supplied by their bacterial host cell.
Bacterial cells can undergo one of two types of infections by viruses termed lytic infections andlysogenic (temperate) infections. In E. coli, lytic infections are caused by a group seven phages known as the T-phages, while lysogenic infections are caused by the phage lambda.
Lytic Infections
The T-phages, T1 through T7, are referred to as lytic phages because they always bring about the lysis and death of their host cell, the bacterium E. coli. T-phages contain double-stranded DNA as their genetic material. In addition to their protein coat or capsid (also referred to as the "head"), T-phages also possess a tail and some related structures. A diagram and electron microgra.
A comprehensive illustration about viruses and their genetic system. The life cycle of bacteriophages. The transfer of their genetic system via the process of transduction (Generalised and Specialised) and studying the gene mapping in phages.
All proteins are formed of 20 amino acids.They are mainly formed of α amino acids (except proline).They have COOH and NH3 on same carbon atom. In physiological conditions both the groups are are completely ionised so an amino acid can act both as acid and base (amphoteric)
ALL ABOUT VITAMINS VITAMIN B6, B7, B12 AND FOLIC ACIDSKYFALL
Vitamins are nutrients which are required in micro grams.They are essential for normal function of the body.They act as cofactors and prosthetic groups for enzymes
Quality control (QC) is a procedure or set of procedures intended to ensure that a manufactured product or performed service adheres to a defined set of quality criteria or meets the requirements of the client or customer. QC is similar to, but not identical with, quality assurance (QA).
QC IN clinical biochemistry labs and hospitals
Nutrition requirements increases tremendously during pregnancy and lactation as the expectant or nursing mother not only has to nourish herself but also growing foetus and the infant who is being breast fed
A chromosome abnormality, disorder, anomaly, aberration, or mutation is a missing, extra, or irregular portion of chromosomal DNA. It can be from an atypical number of chromosomes or a structural abnormality in one or more chromosomes
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
2. INTRODUCTION
Viruses are small, acellular particles that
can replicate only in a host cell.
They are obligatory intracellular
parasites.
They consist of a nucleic acid genome
enclosed in a protective protein shell or
capsid.
3. A BACTERIOPHAGE
Bacteriophage is the
virus that infect
bacteria.
Bacteriophages were
discovered by Frederick
Twort(1915)and Felix
d'Herelle(1917).
4. Bacteriophages have nucleic acid
surrounded by capsid.
The capsid is made up of capsomeres.
Capsomeres consist of protein subunit
known as protomeres.
5. COLIPHAGES
Coliphages were discovered by Herelle.
Bacteriophages attacking E.coli are called
as Coliphages and are designated T-type.
These were numbered T1,T2,T3,.....T17
by Max Delbruck(1938).
T2,T4,T6... are known as T-even phages.
Rest are called as T-odd phages.
6. CYANOPHAGES
Cyanophages were discovered by
Schafferman and Morris(1963).
First cyanophage was discovered from
three blue green algal genera ie,
Lyngbya,Phormedium and Plectonema,
hence also known as LPP-1.
7.
8. STRUCTURE OF A T4 PHAGE
• It contains a head and a tail region.
• Head is icosahedron with about 2000
capsomeres and contain a 53 micrometer long
double stranded DNA in it.
• Tail is helical symmetry with a core tube(80
angstrom in diameter) surrounded by a protein
sheath (consist of 144 subunits arranged in 24
ruins of six subunit each).
• Sheath is connected to collar at its upper end
and a base plate at its lower end.
9. • Base plate is hexagonal with single pin or peg
or spike at each corner.
• A thin tall fiber(1300A long) is also given out
from each corner of base plate.
• Head is prism like hexagonal having
950X650A° dimension.
• Tail is 950A° in length joined to head by neck
and collar.
10.
11. Bacteriophages may be classified into
different morphological class
• A - These are the most complex have
hexagonal head and a rigid tail with a
contractile sheath and tail fibres.
• Example - Coliphages T2,T4,T6
• B - These are similar to A but lack contractile
sheath on the tail and may or may not have
tail fibres.
• Example - Coliphages T1,T5
12. • C - These are same as B type but their tail is
shorter than head.
• Example - Coliphages T3,T7.
• D - These have head made up of large
capsomeres but no tail.
• Example - ¢X 174 ,S13.
• E - These are similar to D type instead they
have small capsomeres.
• Example - f2 , MS2.
13. • F - These are filamentous bacteriophage.
• Example - Coliphages fd , f1.
• G - These have lipid envelope and are
polymorphic.
• Example - MV - L2
14.
15.
16.
17.
18. STEPS INVOLVED IN THE INFECTION
OF BACTERIOPHAGE
• ADSORPTION
• PENETRATION OR INJECTION
• ECLIPSE STAGE
• MATURATION OR VIRUS ASSEMBLY
• LYSIS AND RELEASE OF NEW VIRUSES
19. ADSORPTION
Attachment of phage to the bacteria is known as
adsorption.
This occurs when a phage collides with the host
bacteria.
Phage adsorbs to specific receptors(F pili,
lipoproteins,iron transport protein etc.) on the
bacteria by the tip of the tail.
Example : T4 and T7 Coliphages bind to lipoprotein
receptors.
20.
21. PENETRATION OR INJECTION
• The tail fibres of the virus bend to bring the
spikes and basal plate in contact with the
surface of bacterial wall.
• The sheath is pressed and the viral genome
injected to the bacterial cytoplasm.
• The empty capsid that remains out is called
as the ghost or doughnut.
• This step is active and requires ATP.
22.
23. THE ECLIPSE STAGE
This stage shows following activity in the host
cell : -
• Immunity against infection by same phages
by the production of specific enzyme called
as repressors.
• Suppression of all cellular activity of host.
• Synthesis of proteins coded by phage DNA
using hosts amino acid pool. The protein
synthesized is called as early proteins.
24. • These proteins are enzymes and are used to
seal the hole in cell wall, to destruct the DNA
of host.
• The fresh DNA molecules of phage synthesize
late protein which are the viral coat proteins
and viral lysozymes.
• The coat protein are monomer which are
assembled into capsid and other viral
components.
25.
26. MATURATION OR VIRUS ASSEMBLY
• Head and tail are first assembled separately
then they are attached to form viral particles.
• The time period between injection of viral
nucleic acid and the first appearance of
phage is known as eclipse period.
• Time period between injection of viral
nucleic acid to rupture of host cell is known
as latent period.
27.
28. LYSIS AND RELEASE OF NEW VIRUSES
• The cell wall burst out at the end of latent
period and the phages are liberated .
• This phenomenon is known as lysis.
• The number of virions produced per host cell
is specific and is termed as burst size.
• Generally it is 200 - 300.
29.
30. What are phages good for?
• They play important role in ecology and
evolution of bacteria.
• Phages assist identification, classification and
detection of pathogenic bacteria.
• They are used in phage therapy(in order to
specifically destroy pathogens).
• They eliminate pathogens like Campylobacter
and Listeria in fresh food to reduce food
spoilage.