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RBC FATE OF RBCS ERYTHIOPOIESIS HEMOGLOBIN 1) circular biconcave discs. 2) non nucleated 3) not contain cell organelles 4) are elastic and highly deforming. 5) Life span 120 days In red cell membrane there are very important proteins which maintain the shape of RBCs. These are: 1) Spectrin 2) Ankyrin 3) Stromatin, 4) Actin 5) glycoprotein Elanin. A congenital haemolytic anaemia i.e; hereditary spherocytosis results because of a significant deficiency of spectrin in the wall of RBCs cannot be maintained. In RBCs membrane, blood group antigens are present. SITES Embryonic life (early wks) --- Yolk sac Middle trimester ---- Liver, spleen, Lymph nodes Last months & after birth --- Bone marrow 5 years --- B0ne marrow of all bones Till 20 years --- proximal portions of humeri & tibiae Onwards --- marrow of membranous bones such as vertebrae, sternum, ribs and ilia. (trimester is 3 months duration in pregnancy) The process of the origin, development and maturation of red blood cells. It is an extremely active process About 2.5 million erythrocytes are produced every second in order to replace those that are continuously destroyed by spleen and liver Different growth and differentiation inducers control growth and differentiation of stem cells controlled by factors outside bone marrow Above downward Size of nucleus decrease Size of hemoglobin increase Cytoplasmic/ nucleus ratio increase Amount of RNA responsible for basophilic stain decrease In orthochromatic erythroblast stage , nucleus is expelled. During reticulocyte stage, cell passes from bone marrow in to blood capillaries by the process of “Diapedesis”. Small amount of basophilic material. Remaining basophilic material in the reticulocyte disappear with in 1 to 2 days and then it is called “Erythrocytes”. Life span 120 days After 120 days, taken by reticuloendothelial cells i.e; spleen & liver. Spleen is the graveyard of RBCs. Decrease in enzyme activity, ATP levels, and MCH Decreased deformability Metabolic processes slow down Membrane becomes fragile Destroyed in spleen as the red pulp space is narrow (3 μm) as diameter is very small. So, the spleen is called “Graveyard of RBCs”. RBCs burst out Hb is immediately phagocytized by macrophages pf the body. Iron and globin released. EMBRYO In 2nd month of intrauterine life: There is Gower 1 & Gower 2 Hb. Gower 1 contain: 2 zeta & 2 Epsilon Gower 2 contain: 2 alpha & 2 Epsilon 3rd month onwards: Fetal Hb: Hb-F occurs in fetal red cells and disappears by 2 to 3 months after birth. During I/U life, there is HbF= 2alpha + 2 gamma chains. Adult Haemoglobin (HbA1): 2 alpha chains and 2 beta chains HbA2: is a minor component & is only 2.5% in normal adults. After birth, there is Hb A2 (small amount), which is composed of 2 alpha & 2 delta chains.
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