1. Antifungal and Molecular aspect of
Biosynthesis Selenium Nanoparticles
Anil Kumar
M.Phil. NanoScience
Centre for Nano-Sciences,
Central University of Gujarat,
Gandhinagar. Gujarat.
Email ID: kmr.nano@yahoo.com
Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles: Anil Kumar

2. INTRODUCTION
 Selenium is a chemical element with symbol Se and atomic
number 34. Selenium has six naturally occurring isotopes,
five of which are stable: 74Se, 76Se, 77Se, 78Se, and 80Se.
hexagonal crystal lattice consisting of helical polymeric
chains, wherein the Se-Se distance is 237.3 pm and Se-Se-Se
angle is 130.1 degrees.
 commercial uses for selenium today are in glassmaking and
in pigments. Selenium is a semiconductor and is used in
photocells.
 Selenium are major role in medical field as a drugs against
to Keratin fungus and HIV, Cancer, Tuberculosis, Other
Epidemics Diseases, for beneficial to human and animals
 Selenium salts are toxic in large amounts, but trace amounts
are necessary for cellular function in many organisms,
including all animals.

3. Email ID: kmr.nano@yahoo.com
Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles: Anil Kumar
RESEARCH OBJECT
In recently more cases focused on fungus infections
(Diseases) pose significant global health challenges at world level,
especially in view of the fact that the emergence of resistant
Dermitophytes (keratins) fungus and the adverse side effects
associated with prolonged use continue to slow down the
application of effective antifungal therapies. This makes
imperative the need for the development of safe and potent
alternatives to conventional antifungal drugs. In the present
sequence of events and master plan on Nano scale materials
have emerged as novel antifungal agents for the possibilities
offered by their unique chemical and physical properties.
Selenium nanoparticles have mainly been studied for their
antimicrobial potential may be active against several types of
Fungus.

4. Email ID: kmr.nano@yahoo.com
Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles: Anil Kumar
2013: Nuri Akar,“Investigation of characterization and biofouling properties of
PES membrane containing selenium and copper nanoparticles”
2013: Zahra Bahri Kazempour,”Sub-inhibitory concentration of biogenic
selenium nanoparticles lacks post antifungal effect for Aspergillus niger and
Candida albicans andstimulates the growth of Aspergillus niger”
2013: Ramamurthy C,“Green synthesis and characterization of selenium
nanoparticles and its augmented cytotoxicity with doxorubicin on cancer
cells”
2013: Dungeng Peng,“Size effect of elemental selenium nanoparticles (Nano-
Se) at supranutritional levels on selenium accumulation and glutathione S-
transferase activity”
2013: CH. Ramamurthy, “Green synthesis and characterization of selenium
nanoparticles and its augmented cytotoxicity with doxorubicin on cancer
cells”,
REVIEW LITRATURES:

5. Email ID: kmr.nano@yahoo.com
Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles: Anil Kumar
2012:Hualian Wu,“Surface decoration of selenium nanoparticles by
mushroom polysaccharides–protein complexes to achieve enhanced
cellular uptake and antiproliferative activity”
2012: Mini Bajaj, “Formation of Se (0) Nanoparticles by Duganella sp. and
Agrobacterium sp. Isolated from Se-laden soil of North-East Punjab,
India.
2012: Kiritkumar K.“ERα signaling imparts chemotherapeutic selectivity to
selenium nanoparticles in breast cancer”
2012: Joseph F Ramos, “Cytotoxicity of selenium nanoparticles in rat
dermal fibroblasts”,
2012: Jing Han,“Synthesis and characterization of selenium–chondroitin
sulfate nanoparticles ”
REVIEW LITRATURES CONT………

6. Email ID: kmr.nano@yahoo.com
Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles: Anil Kumar
2011:Gina Marinescu, “Industrial Nutrient Medium Use For Yeast Selenium
Preparation”
2011: Sun, Yan; "Studies of the reduction mechanism of selenium dioxide and
its impact on the microstructure of manganese electrodeposit“
2011: Péter Eszenyi, “Elemental, Nano-Sized (100-500 nm) Selenium
Production by Probiotic Lactic Acid Bacteria”
2011: M. Kargar Razi, “Preparation of nano selenium particles by water
solution phase method from industrial dust”
2011: Huiyu Chen, “Green synthesis and characterization of se nanoparticles
and nanorods”,
REVIEW LITRATURES CONT………

7. Email ID: kmr.nano@yahoo.com
Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles: Anil Kumar
2010: Soniya Dhanjal,“Aerobic biogenesis of selenium nanospheres by
Bacillus cereus isolated from coalmine soil”
2010: Shiho Chiba,”Structural Basis for the Major Role of O-Phosphoseryl-
tRNA Kinase in the UGA-Specific Encoding of Selenocysteine”
2010: Ralston, NVC; "Dietary selenium's protective effects against
methylmercury toxicity“
2010: Parisa Jafari Fesharaki,”Biosynthesis Of Selenium Nanoparticles Using
Klebsiella Pneumoniae And Their Recovery By A Simple Sterilization Process,
Brazilian Journal of Microbiology
2010: Ahmad Reza Shahverdi, “Antifungal Activity of Biogenic
SeleniumNanoparticles”.
REVIEW LITRATURES CONT………

8. Email ID: kmr.nano@yahoo.com
Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles: Anil Kumar
2009: Retrieved,"Selenium(IV)_sulfide"
2008: Zane Davis, T., "Selenium in Plants"
2008: Deutsche,"Copper indium diselenide (CIS) cell".
2007: M. P. Lyons,“Selenium in Food Chain and Animal Nutrition: Lessons
from Nature-Review”
2005: Bernd "Selenium and Selenium Compounds“.
2004: Zong-Hong Lin,“Observation in the Growth of Selenium
Nanoparticles”
2001: Davis, ”Copper and Copper Alloys”.
1999: Gladyshev,"Selenocysteine-containing proteins in mammals".
1995: Barclay, "Selenium content of a range of UK food".
1980: Wilber, C. G., "Toxicology of selenium"
1975: Aleksandrowicz J, “Selenium effect on the growth of carcinogenic
fungi and cytotoxic action of aflatoxin B1 on lymphocyte culture and on
embryonal development of Xenopus laevis”.
REVIEW LITRATURES CONT………

9. Email ID: kmr.nano@yahoo.com
Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles: Anil Kumar
OBJECT & OBJECTIVES
Object:- Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles
 The collection of all sample from selected areas of, INDIA, Procedure for Isolation
of Keratin Fungus from source.
 Isolation of Agrobacterium species from root nodes of plants and soil, growth on
YEMA media contained with sodium selenite both solid and liquid.
 The bacterial cell wall destabilization by lysozymes solution for gain to
Selenocystien Amino Acids.
 Characterization of selenium molecules size and shape in range of nanoscale (1-
100nm) by using instruments of pH, Chromatography’s, Gel Electrophoresis,
viscometer, Spectroscopy, Zeta potential, FTIR, XRD, AFM, TEM, SEM and UHVetc.
Objectives:-

10. Email ID: kmr.nano@yahoo.com
Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles: Anil Kumar
 Purified selenium contained materials to applied Selenium substrate in µg/ml
on Fungus culture.
 Optimization of molecular aspects of selenium extract substance on keratine
Fungus, like as destabilized cellular organelles of keratin fungus .
 To be gained data analysis by statistical tools such as Correlation coeficient,
SD, SEM, t-Test, Chi test, ANOVA, and Graphical Representation.
OBJECT & OBJECTIVES CONT…….
Objectives:-

11. Email ID: kmr.nano@yahoo.com
Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles: Anil Kumar
Selenium in future may be also
apply for Anti-Cancer, Anti
HIV/AIDS, Anti-Dandruffs and
Anti-epidemics, their futures
scope as following ways.
FUTURE EXPECTATION:-

12. Email ID: kmr.nano@yahoo.com
Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles: Anil Kumar
Structure of Selenium Nanoparticles

13. Email ID: kmr.nano@yahoo.com
Antifungal and Molecular aspect of Biogenesis Selenium Nanoparticles: Anil Kumar
APPLICATIONS OF SELENIUM NANOPARTICLES
Manganese electrolysis:- During the electro winning of manganese an addition of
selenium dioxide. China is the largest consumer of selenium dioxide for this purpose. For
every 2 kg selenium oxide is used.
Glass production:- The use of Se, 50% of consumption, is for the production of glass. Se
compounds confer a red color to glass. For this purpose various selenite and selenate
salts are added.
Alloys :- Selenium is used with Bismuth in Brosses to replace more toxic lead in drinking
water a reduction of lead in brass necessary.
Solar cells:- Gallium Selenide is a material used in the production of solar cells.
Nutritional sources of selenium:- Dietary selenium comes from nuts, cereals, meat,
mushrooms, fish, and eggs. Brazil nuts are the richest ordinary dietary source. The
human body's content of selenium is believed to be in the 13–20 milligram range.
Indicator plant species:- Certain species of plants are considered indicators of high
selenium content of the soil, plants are Astragalus sp.,Stanleya sp.), Xylorhiza sp. and
Oonopsis sp etc.
Medicals:- use The Selenium Sulfide (SeS2) is the active ingredient in some anti-dandruff
shampoos. The selenium compound kills the scalp fungus Malassezia, which causes
shedding of dry skin fragments.

14. Isolation of Bacteria From Source
Sub Culture of Keratin fungi
on Synthetic Media
Isolation of Fungus on Keratins
Sub Culture of Agrobacterium
Biosynthesis of Selenium NP
Isolation, purification, and
Quantification selenium
molecules
Formation and Characterization
of Selenium Nanoparticles
Sub culturing of
Fungi on
Solidified Media
Sub culturing of
Fungi on Broth
Media
Applying of Selenium Nanoparticles
on Both Sub Culturing Fungi
Molecular Aspects of SeNP with
Fungi
Observation of effects SeNP on
Fungi
5-7
days
5-7
days
5-7
days
2-3
Days
15-
20
Days
7-10
days
7-10
days
7-12
Days
7-12
Day s
1-2
Days
10-15
Days
EXPERIMENTAL DURATION CHART OF
ANTIFUNGAL & MOLECULAR ASPECT OF BIOGENESIS SELENIUM NANOPARTICLES

15. Thanking All of You….......