This document summarizes the synthesis and characterization of silver nanoparticles and chitosan membranes containing silver nanoparticles for their potential antifungal properties. Key points:
- Silver nanoparticles were synthesized via chemical reduction and characterized through SEM, XRD, EDS and FTIR to confirm size, purity and crystal structure.
- Chitosan membranes were prepared with varying concentrations of silver nanoparticles and glutaraldehyde as a crosslinker. Membrane thickness and structure were analyzed through FTIR and thermal analysis.
- The antifungal activity of silver nanoparticles and chitosan membranes was tested against various wood staining fungi through inhibition zone and radial growth methods to evaluate their potential as wood preservatives.
The document summarizes research characterizing gold nanoparticles synthesized intracellularly by the biomass of Aspergillus terreus fungus. When an aqueous solution of chloroauric acid was reduced by A. terreus biomass, it changed color from yellow to pinkish violet, indicating gold nanoparticle formation. The synthesized nanoparticles were spherical or irregularly shaped with an average size of 186 nm. Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy and X-ray diffraction were used to characterize the nanoparticles and confirmed the presence of crystalline gold nanoparticles capped by biomolecules from the fungus.
Silver Nanoparticles Synthesis, Properties, Applications and Future Perspecti...iosrjce
Silver nanoparticles (Ag NPs) have gained significant interest due to their unique optical,
antimicrobial, electrical, physical properties and their possible application. The change of energy level from
continuous band to discrete band of Ag NPs with decrease in size of particles gives strong size dependent
chemical and physical properties. Ag NPs show lower toxicity to human health while Ag NPs show higher
toxicity to various micro-organisms. For this reason Ag NPs having scope for medical instruments,
antimicrobial application, products for health care such as scaffolds, burn dressing, water purification,
agriculture uses. Ag NPs can be synthesized by using various methods which is primarily classified into two
type’s namely physical process which includes laser ablation, condensation, evaporation etc. and chemical
process which includes hydrazine, sodium borohydride, green synthesis etc. Among all these methods green
synthesis is non-toxic, eco-friendly and cost effective. In this review paper different synthesis process especially
green synthesis, properties, applications of silver nanoparticles and their recent advances are described. We
also highlight the toxicity and compares Ag NPs with others nanoparticles.
Synthesis of the silver nanoparticles from Aloe barbadensis extract and its a...IRJET Journal
This document summarizes a study that synthesized silver nanoparticles from Aloe barbadensis leaf extract and evaluated their antimicrobial activity against urinary tract infection pathogens. Key findings include:
- Silver nanoparticles were successfully synthesized using Aloe barbadensis leaf extract and characterized using UV-Vis spectroscopy and FTIR.
- Thirty-two bacteria isolated from urine samples were identified, with Escherichia coli being the most prevalent pathogen identified in 47% of samples.
- Disc diffusion assays found the silver nanoparticles showed strongest antimicrobial activity against E. coli, followed by Candida albicans. Klebsiella pneumoniae and Staphylococcus aureus showed no sensitivity.
- Results indicate silver
Silver nanoparticles from the leaf extract of datura metelUsman Arshad
This research article describes the green synthesis and characterization of zero-valent silver nanoparticles using the leaf extract of the Datura metel plant. Silver nanoparticles were synthesized by mixing an aqueous solution of silver nitrate with an extract of D. metel leaves. Characterization using UV-Vis spectroscopy, SEM, and TEM showed the particles were predominantly between 50-100 nm in size. Antimicrobial testing demonstrated the silver nanoparticles had inhibitory effects against bacterial and fungal strains. The green synthesis method provides a low-cost and environmentally friendly approach for producing silver nanoparticles.
Biosynthesis of Silver Nanoparticles (AgNPs) and it's ApplicationsManish Dash
The document discusses the green synthesis and applications of silver nanoparticles. It begins by outlining the need for advanced disinfectant nanomaterials to prevent disease outbreaks caused by overpopulation and poor sanitation. It then describes how silver nanoparticles are a promising material for developing antimicrobial products due to their high antimicrobial activity. The document goes on to detail a green synthesis method for producing silver nanoparticles using the extract of Bougainvillea plant bracts, and characterizes the nanoparticles. It finds that the synthesized silver nanoparticles demonstrate effective antimicrobial properties against bacteria as well as antifouling effects.
This document summarizes research on the green synthesis of nanoparticles using plants. It discusses how plants provide an eco-friendly alternative to physical and chemical synthesis methods. Key points include:
- Plants can synthesize nanoparticles both in the laboratory and natural environment through biosynthesis, involving the reduction of metal ions.
- Factors like temperature, pH, and ion concentrations can influence nanoparticle size, shape, and properties.
- Plants have synthesized various nanoparticles including silver, gold, silicon-germanium, and magnetite which have applications in areas like catalysis, sensing, and medicine.
- Silver nanoparticles from various plants have demonstrated antimicrobial properties against bacteria like E. coli, making them promising for applications like
The current research aimed at fabricating plant extract mediated biosynthesized silver nanoparticles (AgNPs) utilizing thorn extract of Bombax ceiba (TEBC). The synthesized AgNPs was characterized by UV spectroscopy where the surface plasmonic resonance peak (SPR) was located at 222 nm. The scanning electron microscopy (SEM) studies demonstrated that the morphology of fabricated nanomaterials was primarily cylindrical of average size of 20-30 nm with some spindles of size >50 nm. The anti-microbial evaluation against Staphylococcus aureus revealed that AgNPs exhibited notable activity with ZOI of 27.2 mm at MIC of 25 μg/mL. The outcome of this research evidently signified that the biofabricated AgNPs using TEBC may be a new greener approach or technology to formulate anti-bacterial nanodrugs in future.
The document summarizes research characterizing gold nanoparticles synthesized intracellularly by the biomass of Aspergillus terreus fungus. When an aqueous solution of chloroauric acid was reduced by A. terreus biomass, it changed color from yellow to pinkish violet, indicating gold nanoparticle formation. The synthesized nanoparticles were spherical or irregularly shaped with an average size of 186 nm. Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy and X-ray diffraction were used to characterize the nanoparticles and confirmed the presence of crystalline gold nanoparticles capped by biomolecules from the fungus.
Silver Nanoparticles Synthesis, Properties, Applications and Future Perspecti...iosrjce
Silver nanoparticles (Ag NPs) have gained significant interest due to their unique optical,
antimicrobial, electrical, physical properties and their possible application. The change of energy level from
continuous band to discrete band of Ag NPs with decrease in size of particles gives strong size dependent
chemical and physical properties. Ag NPs show lower toxicity to human health while Ag NPs show higher
toxicity to various micro-organisms. For this reason Ag NPs having scope for medical instruments,
antimicrobial application, products for health care such as scaffolds, burn dressing, water purification,
agriculture uses. Ag NPs can be synthesized by using various methods which is primarily classified into two
type’s namely physical process which includes laser ablation, condensation, evaporation etc. and chemical
process which includes hydrazine, sodium borohydride, green synthesis etc. Among all these methods green
synthesis is non-toxic, eco-friendly and cost effective. In this review paper different synthesis process especially
green synthesis, properties, applications of silver nanoparticles and their recent advances are described. We
also highlight the toxicity and compares Ag NPs with others nanoparticles.
Synthesis of the silver nanoparticles from Aloe barbadensis extract and its a...IRJET Journal
This document summarizes a study that synthesized silver nanoparticles from Aloe barbadensis leaf extract and evaluated their antimicrobial activity against urinary tract infection pathogens. Key findings include:
- Silver nanoparticles were successfully synthesized using Aloe barbadensis leaf extract and characterized using UV-Vis spectroscopy and FTIR.
- Thirty-two bacteria isolated from urine samples were identified, with Escherichia coli being the most prevalent pathogen identified in 47% of samples.
- Disc diffusion assays found the silver nanoparticles showed strongest antimicrobial activity against E. coli, followed by Candida albicans. Klebsiella pneumoniae and Staphylococcus aureus showed no sensitivity.
- Results indicate silver
Silver nanoparticles from the leaf extract of datura metelUsman Arshad
This research article describes the green synthesis and characterization of zero-valent silver nanoparticles using the leaf extract of the Datura metel plant. Silver nanoparticles were synthesized by mixing an aqueous solution of silver nitrate with an extract of D. metel leaves. Characterization using UV-Vis spectroscopy, SEM, and TEM showed the particles were predominantly between 50-100 nm in size. Antimicrobial testing demonstrated the silver nanoparticles had inhibitory effects against bacterial and fungal strains. The green synthesis method provides a low-cost and environmentally friendly approach for producing silver nanoparticles.
Biosynthesis of Silver Nanoparticles (AgNPs) and it's ApplicationsManish Dash
The document discusses the green synthesis and applications of silver nanoparticles. It begins by outlining the need for advanced disinfectant nanomaterials to prevent disease outbreaks caused by overpopulation and poor sanitation. It then describes how silver nanoparticles are a promising material for developing antimicrobial products due to their high antimicrobial activity. The document goes on to detail a green synthesis method for producing silver nanoparticles using the extract of Bougainvillea plant bracts, and characterizes the nanoparticles. It finds that the synthesized silver nanoparticles demonstrate effective antimicrobial properties against bacteria as well as antifouling effects.
This document summarizes research on the green synthesis of nanoparticles using plants. It discusses how plants provide an eco-friendly alternative to physical and chemical synthesis methods. Key points include:
- Plants can synthesize nanoparticles both in the laboratory and natural environment through biosynthesis, involving the reduction of metal ions.
- Factors like temperature, pH, and ion concentrations can influence nanoparticle size, shape, and properties.
- Plants have synthesized various nanoparticles including silver, gold, silicon-germanium, and magnetite which have applications in areas like catalysis, sensing, and medicine.
- Silver nanoparticles from various plants have demonstrated antimicrobial properties against bacteria like E. coli, making them promising for applications like
The current research aimed at fabricating plant extract mediated biosynthesized silver nanoparticles (AgNPs) utilizing thorn extract of Bombax ceiba (TEBC). The synthesized AgNPs was characterized by UV spectroscopy where the surface plasmonic resonance peak (SPR) was located at 222 nm. The scanning electron microscopy (SEM) studies demonstrated that the morphology of fabricated nanomaterials was primarily cylindrical of average size of 20-30 nm with some spindles of size >50 nm. The anti-microbial evaluation against Staphylococcus aureus revealed that AgNPs exhibited notable activity with ZOI of 27.2 mm at MIC of 25 μg/mL. The outcome of this research evidently signified that the biofabricated AgNPs using TEBC may be a new greener approach or technology to formulate anti-bacterial nanodrugs in future.
The review article summarizes the applications of silver nanoparticles for diverse sectors. Over the decades, nanoparticles used as dignified metals such as silver exhibited distinctive characteristics basically correlated
to chemical, physical and biological property of counterparts having bulkiness. Numerous studies reported that Nanoparticles of about 100 nm diameter play a crucial role in widely spread industries due to unique properties including the dimension of small particle, high surface area and quantum confinement and they dispersed without agglomeration. Decade of discoveries clearly established that shape, size and distribution of Silver nanoparticles strongly affect the electromagnetic, optical and catalytic properties, which are often an assortment of changeable synthetic methods and reducing agents with stabilizers. Generation after generation the postulates come forth about properties of silver for the ancient Greeks cook from silver pots and the old adage ‘born with a silver spoon in his mouth’ thus show that eating with a silver spoon was wellknown
as uncontaminated. Impregnation of metals with silver nanoparticles is a practical way to exploit the microbe aggressive properties of silver at very low cost. The nanoparticles help in targeted delivery of drugs, enhancing bioavailability, sustaining drug or gene effect in target tissues, and enhancing the stability. Implementations of silver partials in medical science and biological science have been noticed from years ago; however alteration with nanotechnology is innovative potential. Over 23% of all nanotechnology based products, diagnostic and therapeutic applications implanted with silver nanoparticles (e.g. In arthritic disease and wound healing, etc.) and widely known for their antifungal, antibacterial, antiviral effect, employed in textile fabrics and added into cosmetic products as antiseptic to overcome skin problems. Thus, Silver
nanoparticles (AgNPs) have been urbanized as an advanced artifact in the field of nanotechnology.
Green synthesis of zinc oxide nano particles using flower extract cassia dens...IJERD Editor
Green synthesis of metal nanoparticles is an interesting issue of the nanoscience and
nanobiotechnology. There is a growing attention to biosynthesis the metal nanoparticles using organisms.
Among these organisms, plants seem to be the best and they are suitable for large scale biosynthesis of
nanoparticles. Nanoparticles produced by plants are more stable, and the rate of synthesis is faster than that in
the case of other organisms. The present investigation was carried out to green synthesis of zinc oxide
nanoparticles by using the medicinal plant cassia densistipulata taub. The flower was collected from the campus
of Anantapuramu, Andhra Pradesh and their petals were separated. The petals were taken and cleaned with
dimeneralized water and soaked for an hour on dry cloth to remove moisture from the petals.
Synthesis of Zinc Nanoparticles was done by mixing 5gms of Zinc Nitrate with 50ml of aqueous
extract of cassia densistipulata taub petals. The formation of nanoparticles was monitored by visualizing color
changes and it was confirmed by Electron microscope (SEM), UV-Vis spectrophotometer and Fourier
Transform Infra-Red (FT-IR) spectroscopy. The results of various techniques confirmed the presence Zinc oxide
nanoparticles.
Green Synthesis Of Silver NanoparticlesAnal Mondal
This document discusses the green synthesis of silver nanoparticles. It begins by defining nanoparticles and describing their properties. It then discusses silver nanoparticles specifically, including their size range and color properties. The rest of the document discusses the green synthesis technique for producing silver nanoparticles using plant extracts, the advantages of this method over chemical synthesis, and various characterization techniques and applications of the synthesized silver nanoparticles.
1 biosynthesis of nanoparticles - algaeHamid Salari
This document discusses the biological synthesis of silver nanoparticles using algae as a non-toxic and environmentally friendly method. It outlines three methods for nanoparticle synthesis: physical, chemical, and biological. The biological approach uses algae such as Capillacae, J. Rubins, C. Sinusa, and Faciata to extract polysaccharides and synthesize silver nanoparticles, which are then characterized through techniques like TEM, FTIR, and UV-visible spectral analysis. The results show the potential for using algae-mediated biosynthesis of silver nanoparticles for applications such as antimicrobial textile treatment.
It an overall view on two research papers. Biological synthesis of Nano particles from plants and microorganisms
and the synthesis of metallic Nano particles using plant extract
Chemo bio synthesis of silver nanoparticlesJagpreet Singh
Silver nanoparticles have a lot of ways of synthesis like physical and chemical
methods; some of these methods use a lot of chemical substances and are
very hazardous for humans and environment, so a novel, great, environmental
friendly, cheap and easy to use world of green chemistry has been used. A
number of characterization techniques such as UV-visible spectroscopy, Fourier
transformation infrared spectroscopy, X-ray diffraction study and scanning
electron microscopy revealed that silver nanoparticles have been used. Thus
the different response of the functional groups and the difference in the peaks
and UV-visible data was studied and then compared to understand and know
the way these different reducing agents react to the same starting material. The
green synthesis had a UV-visible peak at 446 nm while the one with chemical
synthesis had a peak at 395 nm. FTIR results of silver nanoparticles synthesis
by trisodium citrate (TSC) showed a peak at 1505 cm-1 which shows that the
compound has a stretching of the -C=C – bond. In another case, which was done
by using Sodium borohydride (NaBH4) a peak at 1695 cm-1 showed a –C=O- bond
indicating stretching and a weak absorption intensity. Another peak was present
which indicates a –O-H bond formation and presence which is a strong bond are
found to exist. A notable peak came for synthesis by orange peel at 1517 cm-1
which represents a –C=C- bond stretching as in aromatic compounds. Another
peak at 1732 cm-1 indicates the –C=O- bond. The XRD results on one of the
silver sample prepared by green methods showed silver nanomaterials formed
which had a average particle size of around 42 nm. FE-SEM results revealed that
silver nanomaterials were formed and had a flake like appearance in one of the
results. All the overall comparison showed that different modes of synthesis
of silver nanomaterials and different reducing agents give same materials but
with different peaks and intensities. All this data provided knowledge about the
fact that an alternative method can be used to create new nanoparticles if one
of the previously considered to tried method fails thus helping in extending the
broadways for research.
This document summarizes the green synthesis of silver nanorods and wires using plant extracts from Zoysia japonica lawn grass, Azadarichta indica neem leaves, and Prunus dulcis almond. The aim was to produce silver nanoparticles using these plant extracts as reducing agents. Silver nanoparticles were synthesized by adding plant extracts to a silver nitrate solution and incubating for 24 hours, resulting in color changes. The synthesized nanoparticles were characterized using UV-visible spectroscopy, which showed absorption peaks varying between 300-500 nm depending on the plant extract used. Further analysis using techniques like iodometric titration, TEM, and FTIR were proposed to understand stabilization and structure of the synthesized silver nanoparticles.
Review on green synthesis of silver nanoparticles using plant extract. Various green materials are used for the synthesis of Ag. Several synthesis method main emphasis on green method.
Is Nano Medicine And Nano Technology The Most Trending Thing Now?science journals
Nano medicine is nothing but application of Nano technologies as medicines. It may include application of non-material as biological devices or nano-electronic biosensors. Molecular nanotechnology as biological machines may have medical applications in future.
Biosynthesis and Characterization of Zinc Oxide Nanoparticles using Onion Bul...ijtsrd
The wide application of nanoparticles stimulates the need for synthesizing them but, the conventional methods are usually hazardous and energy consuming. This leads to focus on œgreen synthesis of nanoparticles which seems to be easy efficient and ecofriendly approach. In this study, the plant mediated synthesis of zinc oxide nanoparticles (ZnO NPs) was carried out using bulb extract of Allium cepa as a reducing agent. The optimized nano zinc thus obtained was quantified and characterized using UV-Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray diffraction, Scanning Electron Microscope (SEM), EDAX and Zeta potential analyses. Further, the synthesized ZnO NPs were tested for antimicrobial activity. N. Tensingh Baliah | S. Lega Priyatharsini"Biosynthesis and Characterization of Zinc Oxide Nanoparticles using Onion Bulb Extract" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018, URL: http://www.ijtsrd.com/papers/ijtsrd8305.pdf http://www.ijtsrd.com/biological-science/botany/8305/biosynthesis-and-characterization-of-zinc-oxide--nanoparticles-using-onion-bulb-extract/n-tensingh-baliah
This document summarizes a study that used the leaf extract of Hibiscus rosa sinensis to biologically synthesize gold and silver nanoparticles. Varying experimental conditions such as the ratio of metal salts to leaf extract and pH allowed modulation of the nanoparticles' size, shape, and properties. Nanoparticles were characterized using techniques like UV-vis spectroscopy, transmission electron microscopy, X-ray diffraction, and FTIR spectroscopy. Results confirmed the production of crystalline gold and silver nanoparticles in the face-centered cubic structure, as well as the ability to control nanoparticle shape through simple adjustments to the synthesis method. This green chemistry approach provides a simple, cost-effective way to produce metal nanoparticles with potential applications.
The document summarizes a study that biologically synthesized and characterized intracellular gold nanoparticles using the biomass of Aspergillus fumigatus. A. fumigatus was grown in liquid culture and its biomass was then exposed to chloroauric acid solution, resulting in the intracellular production of gold nanoparticles within 72 hours, as indicated by a color change. The synthesized nanoparticles were characterized through various techniques and were found to be spherical and irregularly shaped, ranging from 85.1 to 210 nm in size. Scanning electron microscopy revealed the nanoparticles accumulated on the fungal mycelia. Energy dispersive spectroscopy and X-ray diffraction confirmed the presence and crystalline nature of the synthesized gold nanoparticles.
This document discusses the antimicrobial activity of silver nanoparticles. It notes that microbes are causing diseases and developing antimicrobial resistance, posing problems. Silver has long been known to have medical antimicrobial properties. Silver nanoparticles have unique properties that make them useful for molecular diagnostics and therapies. Their exact antimicrobial mechanism is debated, but they may accumulate on cell surfaces, release silver ions, generate reactive oxygen species, react with cellular components like DNA, and modulate bacterial signal transduction, ultimately killing microbes through several potential mechanisms. While silver has been an excellent antimicrobial, nanoparticles may increase its efficacy, though more research is needed to fully understand their antimicrobial activity.
Synthesis of Silver Nano Particles from Marine Bacteria Pseudomonas aerogenosaKamalpreet Sarna
This document summarizes a study that isolated a marine bacterial strain called Pseudomonas aeruginosa and used it to synthesize silver nanoparticles. The silver nanoparticles were characterized using UV-Vis spectroscopy, SEM, FTIR, and XRD. UV-Vis analysis showed a peak at 420nm indicating the presence of silver nanoparticles. SEM images showed the nanoparticles were spherical in shape with sizes ranging from 50-80nm. FTIR and XRD further confirmed the presence of silver. The silver nanoparticles showed potent antibacterial activity against both gram-positive and gram-negative bacteria as well as antifungal activity. This study demonstrates the potential of using marine bacteria as a green synthesis method for producing silver nanoparticles with biological applications.
This presentation discusses green synthesis of nanoparticles using biological methods. It notes that physical and chemical synthesis methods can be time consuming, require high temperatures/pressures, and use toxic chemicals. Green synthesis utilizes natural reducing, capping and stabilizing agents from plants and microorganisms to synthesize nanoparticles without toxic chemicals or high energy requirements. Specific methods discussed include using plant extracts like aloe vera to synthesize gold nanoparticles and citrus peels to synthesize silver nanoparticles. The mechanisms of plant-mediated green synthesis and an example using phlomis leaf extract to synthesize silver nanoparticles are also summarized.
International Journal of Engineering and Science Invention (IJESI) is an international journal intended for professionals and researchers in all fields of computer science and electronics. IJESI publishes research articles and reviews within the whole field Engineering Science and Technology, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Biological methods can be used to remove heavy metals from wastewater. Biosorption uses pretreated algal biomass to adsorb metals like selenium, lead, and cadmium. The fixed activated sludge process (FAST) uses submerged fixed media to increase surface area and resist shock loads, improving removal of metals like lead, chromium, and nickel. Bioleaching extracts metals from sewage sludge using bacteria at low pH and high iron concentrations over 4-10 days, increasing removal of chromium, copper, and zinc. Extracellular polymeric substances from Cloacibacterium normanense removed 85% nickel and 72% aluminum from wastewater.
Green synthesis and characterization of silver nanoparticles using tinosopora...IJARIIT
The document summarizes research on the green synthesis of silver nanoparticles using an extract of Tinospora cordifolia and their characterization and antimicrobial activity. Key findings include:
- Silver nanoparticles ranging from 27-58 nm were successfully synthesized using a T. cordifolia extract, as confirmed by UV-Vis and SEM analysis.
- XRD and FTIR analysis revealed the nanoparticles were crystalline in nature and that biomolecules in the plant extract were responsible for silver ion reduction and nanoparticle stabilization.
- The synthesized silver nanoparticles demonstrated antimicrobial activity against several pathogenic bacteria, indicating potential applications in medicine, food, and cosmetics.
synthesis of nanoparticles by green chemistry approach and their anticancer a...Gulzar Ahmed Rather
1) Klebsiella pneumonia was used to biologically synthesize gold nanoparticles, as indicated by a color change from white to yellow to dark purple.
2) The gold nanoparticles were characterized using UV-visible spectroscopy, FE-SEM, and XRD, and were spherical in shape with sizes ranging from 40-60nm.
3) The biologically synthesized gold nanoparticles showed antibacterial activity against several human pathogens, including Staphylococcus aureus and Escherichia coli, as measured using the disc diffusion method.
This PowerPoint is one small part of the Taxonomy and Classification unit from www.sciencepowerpoint.com. A 3800+ slide Five Part PowerPoint presentation becomes the roadmap for an amazing and interactive science experience full of built-in lab activities, built-in quizzes, video links, class notes(red slides),review games, projects, unit notes, answer keys, and much more. Also included is a student version of the unit that is much like the teachers but missing the answer keys, quizzes, PowerPoint review games, hidden box challenges, owl, and surprises meant for the classroom. This is a great resource to distribute to your students and support professionals. The Classification and Taxonomy Unit covers topics associated with Taxonomy and Classification. The unit examines all of the Kingdoms of Life in detail. Areas of Focus within The Taxonomy and Classification Unit: -Taxonomy, Classification, Need for Taxonomy vs. Common Names, What is a Species?, Dichotomous Keys, What does Classification Use?, The Domains of Life, Kingdoms of Life,The 8 Taxonomic Ranks, Humans Taxonomic Classification, Kingdom Monera, Prokaryotic Cells, Types of Eubacteria, Bacteria Classification, Gram Staining,Bacterial Food Borne Illnesses, Penicillin and Antiseptic, Oral Hygiene and Plaque, Bacterial Reproduction (Binary Fission), Asexual Reproduction, Positives and Negatives of Bacteria, Protista, Plant-like Protists, Animal-like Protists, Fungi-like Protists, Animalia, Characteristics of Animalia, Animal Symmetry, Phylums of Animalia (Extensive), Classes of Chordata, Mammals, Subclasses of Mammals, Characteristics of Mammals, Fungi, Positives and Negatives of Fungi, Divisions of Fungi (Extensive), Parts of a Mushroom, 3 Roles of Fungi, Fungi Reproduction, Mold Prevention, Plant Divisions, Kingdom Plantae. If you have any questions please feel free to contact me. Thanks again and best wishes. Sincerely, Ryan Murphy www.sciencepowerpoint@gmail.com
This document provides an overview of fungal infections (mycoses) including definitions, classifications, pathogenesis, clinical manifestations, laboratory diagnosis and examples. It discusses superficial mycoses including pityriasis versicolor caused by Malassezia furfur. It also discusses cutaneous mycoses such as dermatophytosis caused by fungi like Trichophyton, Epidermatophyton and Microsporum. Deep mycoses caused by fungi that infect internal organs are also described, including histoplasmosis caused by Histoplasma capsulatum, cryptococcosis caused by Cryptococcus neoformans, aspergillosis caused by Aspergillus fumigatus, mucormycosis
The review article summarizes the applications of silver nanoparticles for diverse sectors. Over the decades, nanoparticles used as dignified metals such as silver exhibited distinctive characteristics basically correlated
to chemical, physical and biological property of counterparts having bulkiness. Numerous studies reported that Nanoparticles of about 100 nm diameter play a crucial role in widely spread industries due to unique properties including the dimension of small particle, high surface area and quantum confinement and they dispersed without agglomeration. Decade of discoveries clearly established that shape, size and distribution of Silver nanoparticles strongly affect the electromagnetic, optical and catalytic properties, which are often an assortment of changeable synthetic methods and reducing agents with stabilizers. Generation after generation the postulates come forth about properties of silver for the ancient Greeks cook from silver pots and the old adage ‘born with a silver spoon in his mouth’ thus show that eating with a silver spoon was wellknown
as uncontaminated. Impregnation of metals with silver nanoparticles is a practical way to exploit the microbe aggressive properties of silver at very low cost. The nanoparticles help in targeted delivery of drugs, enhancing bioavailability, sustaining drug or gene effect in target tissues, and enhancing the stability. Implementations of silver partials in medical science and biological science have been noticed from years ago; however alteration with nanotechnology is innovative potential. Over 23% of all nanotechnology based products, diagnostic and therapeutic applications implanted with silver nanoparticles (e.g. In arthritic disease and wound healing, etc.) and widely known for their antifungal, antibacterial, antiviral effect, employed in textile fabrics and added into cosmetic products as antiseptic to overcome skin problems. Thus, Silver
nanoparticles (AgNPs) have been urbanized as an advanced artifact in the field of nanotechnology.
Green synthesis of zinc oxide nano particles using flower extract cassia dens...IJERD Editor
Green synthesis of metal nanoparticles is an interesting issue of the nanoscience and
nanobiotechnology. There is a growing attention to biosynthesis the metal nanoparticles using organisms.
Among these organisms, plants seem to be the best and they are suitable for large scale biosynthesis of
nanoparticles. Nanoparticles produced by plants are more stable, and the rate of synthesis is faster than that in
the case of other organisms. The present investigation was carried out to green synthesis of zinc oxide
nanoparticles by using the medicinal plant cassia densistipulata taub. The flower was collected from the campus
of Anantapuramu, Andhra Pradesh and their petals were separated. The petals were taken and cleaned with
dimeneralized water and soaked for an hour on dry cloth to remove moisture from the petals.
Synthesis of Zinc Nanoparticles was done by mixing 5gms of Zinc Nitrate with 50ml of aqueous
extract of cassia densistipulata taub petals. The formation of nanoparticles was monitored by visualizing color
changes and it was confirmed by Electron microscope (SEM), UV-Vis spectrophotometer and Fourier
Transform Infra-Red (FT-IR) spectroscopy. The results of various techniques confirmed the presence Zinc oxide
nanoparticles.
Green Synthesis Of Silver NanoparticlesAnal Mondal
This document discusses the green synthesis of silver nanoparticles. It begins by defining nanoparticles and describing their properties. It then discusses silver nanoparticles specifically, including their size range and color properties. The rest of the document discusses the green synthesis technique for producing silver nanoparticles using plant extracts, the advantages of this method over chemical synthesis, and various characterization techniques and applications of the synthesized silver nanoparticles.
1 biosynthesis of nanoparticles - algaeHamid Salari
This document discusses the biological synthesis of silver nanoparticles using algae as a non-toxic and environmentally friendly method. It outlines three methods for nanoparticle synthesis: physical, chemical, and biological. The biological approach uses algae such as Capillacae, J. Rubins, C. Sinusa, and Faciata to extract polysaccharides and synthesize silver nanoparticles, which are then characterized through techniques like TEM, FTIR, and UV-visible spectral analysis. The results show the potential for using algae-mediated biosynthesis of silver nanoparticles for applications such as antimicrobial textile treatment.
It an overall view on two research papers. Biological synthesis of Nano particles from plants and microorganisms
and the synthesis of metallic Nano particles using plant extract
Chemo bio synthesis of silver nanoparticlesJagpreet Singh
Silver nanoparticles have a lot of ways of synthesis like physical and chemical
methods; some of these methods use a lot of chemical substances and are
very hazardous for humans and environment, so a novel, great, environmental
friendly, cheap and easy to use world of green chemistry has been used. A
number of characterization techniques such as UV-visible spectroscopy, Fourier
transformation infrared spectroscopy, X-ray diffraction study and scanning
electron microscopy revealed that silver nanoparticles have been used. Thus
the different response of the functional groups and the difference in the peaks
and UV-visible data was studied and then compared to understand and know
the way these different reducing agents react to the same starting material. The
green synthesis had a UV-visible peak at 446 nm while the one with chemical
synthesis had a peak at 395 nm. FTIR results of silver nanoparticles synthesis
by trisodium citrate (TSC) showed a peak at 1505 cm-1 which shows that the
compound has a stretching of the -C=C – bond. In another case, which was done
by using Sodium borohydride (NaBH4) a peak at 1695 cm-1 showed a –C=O- bond
indicating stretching and a weak absorption intensity. Another peak was present
which indicates a –O-H bond formation and presence which is a strong bond are
found to exist. A notable peak came for synthesis by orange peel at 1517 cm-1
which represents a –C=C- bond stretching as in aromatic compounds. Another
peak at 1732 cm-1 indicates the –C=O- bond. The XRD results on one of the
silver sample prepared by green methods showed silver nanomaterials formed
which had a average particle size of around 42 nm. FE-SEM results revealed that
silver nanomaterials were formed and had a flake like appearance in one of the
results. All the overall comparison showed that different modes of synthesis
of silver nanomaterials and different reducing agents give same materials but
with different peaks and intensities. All this data provided knowledge about the
fact that an alternative method can be used to create new nanoparticles if one
of the previously considered to tried method fails thus helping in extending the
broadways for research.
This document summarizes the green synthesis of silver nanorods and wires using plant extracts from Zoysia japonica lawn grass, Azadarichta indica neem leaves, and Prunus dulcis almond. The aim was to produce silver nanoparticles using these plant extracts as reducing agents. Silver nanoparticles were synthesized by adding plant extracts to a silver nitrate solution and incubating for 24 hours, resulting in color changes. The synthesized nanoparticles were characterized using UV-visible spectroscopy, which showed absorption peaks varying between 300-500 nm depending on the plant extract used. Further analysis using techniques like iodometric titration, TEM, and FTIR were proposed to understand stabilization and structure of the synthesized silver nanoparticles.
Review on green synthesis of silver nanoparticles using plant extract. Various green materials are used for the synthesis of Ag. Several synthesis method main emphasis on green method.
Is Nano Medicine And Nano Technology The Most Trending Thing Now?science journals
Nano medicine is nothing but application of Nano technologies as medicines. It may include application of non-material as biological devices or nano-electronic biosensors. Molecular nanotechnology as biological machines may have medical applications in future.
Biosynthesis and Characterization of Zinc Oxide Nanoparticles using Onion Bul...ijtsrd
The wide application of nanoparticles stimulates the need for synthesizing them but, the conventional methods are usually hazardous and energy consuming. This leads to focus on œgreen synthesis of nanoparticles which seems to be easy efficient and ecofriendly approach. In this study, the plant mediated synthesis of zinc oxide nanoparticles (ZnO NPs) was carried out using bulb extract of Allium cepa as a reducing agent. The optimized nano zinc thus obtained was quantified and characterized using UV-Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray diffraction, Scanning Electron Microscope (SEM), EDAX and Zeta potential analyses. Further, the synthesized ZnO NPs were tested for antimicrobial activity. N. Tensingh Baliah | S. Lega Priyatharsini"Biosynthesis and Characterization of Zinc Oxide Nanoparticles using Onion Bulb Extract" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018, URL: http://www.ijtsrd.com/papers/ijtsrd8305.pdf http://www.ijtsrd.com/biological-science/botany/8305/biosynthesis-and-characterization-of-zinc-oxide--nanoparticles-using-onion-bulb-extract/n-tensingh-baliah
This document summarizes a study that used the leaf extract of Hibiscus rosa sinensis to biologically synthesize gold and silver nanoparticles. Varying experimental conditions such as the ratio of metal salts to leaf extract and pH allowed modulation of the nanoparticles' size, shape, and properties. Nanoparticles were characterized using techniques like UV-vis spectroscopy, transmission electron microscopy, X-ray diffraction, and FTIR spectroscopy. Results confirmed the production of crystalline gold and silver nanoparticles in the face-centered cubic structure, as well as the ability to control nanoparticle shape through simple adjustments to the synthesis method. This green chemistry approach provides a simple, cost-effective way to produce metal nanoparticles with potential applications.
The document summarizes a study that biologically synthesized and characterized intracellular gold nanoparticles using the biomass of Aspergillus fumigatus. A. fumigatus was grown in liquid culture and its biomass was then exposed to chloroauric acid solution, resulting in the intracellular production of gold nanoparticles within 72 hours, as indicated by a color change. The synthesized nanoparticles were characterized through various techniques and were found to be spherical and irregularly shaped, ranging from 85.1 to 210 nm in size. Scanning electron microscopy revealed the nanoparticles accumulated on the fungal mycelia. Energy dispersive spectroscopy and X-ray diffraction confirmed the presence and crystalline nature of the synthesized gold nanoparticles.
This document discusses the antimicrobial activity of silver nanoparticles. It notes that microbes are causing diseases and developing antimicrobial resistance, posing problems. Silver has long been known to have medical antimicrobial properties. Silver nanoparticles have unique properties that make them useful for molecular diagnostics and therapies. Their exact antimicrobial mechanism is debated, but they may accumulate on cell surfaces, release silver ions, generate reactive oxygen species, react with cellular components like DNA, and modulate bacterial signal transduction, ultimately killing microbes through several potential mechanisms. While silver has been an excellent antimicrobial, nanoparticles may increase its efficacy, though more research is needed to fully understand their antimicrobial activity.
Synthesis of Silver Nano Particles from Marine Bacteria Pseudomonas aerogenosaKamalpreet Sarna
This document summarizes a study that isolated a marine bacterial strain called Pseudomonas aeruginosa and used it to synthesize silver nanoparticles. The silver nanoparticles were characterized using UV-Vis spectroscopy, SEM, FTIR, and XRD. UV-Vis analysis showed a peak at 420nm indicating the presence of silver nanoparticles. SEM images showed the nanoparticles were spherical in shape with sizes ranging from 50-80nm. FTIR and XRD further confirmed the presence of silver. The silver nanoparticles showed potent antibacterial activity against both gram-positive and gram-negative bacteria as well as antifungal activity. This study demonstrates the potential of using marine bacteria as a green synthesis method for producing silver nanoparticles with biological applications.
This presentation discusses green synthesis of nanoparticles using biological methods. It notes that physical and chemical synthesis methods can be time consuming, require high temperatures/pressures, and use toxic chemicals. Green synthesis utilizes natural reducing, capping and stabilizing agents from plants and microorganisms to synthesize nanoparticles without toxic chemicals or high energy requirements. Specific methods discussed include using plant extracts like aloe vera to synthesize gold nanoparticles and citrus peels to synthesize silver nanoparticles. The mechanisms of plant-mediated green synthesis and an example using phlomis leaf extract to synthesize silver nanoparticles are also summarized.
International Journal of Engineering and Science Invention (IJESI) is an international journal intended for professionals and researchers in all fields of computer science and electronics. IJESI publishes research articles and reviews within the whole field Engineering Science and Technology, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Biological methods can be used to remove heavy metals from wastewater. Biosorption uses pretreated algal biomass to adsorb metals like selenium, lead, and cadmium. The fixed activated sludge process (FAST) uses submerged fixed media to increase surface area and resist shock loads, improving removal of metals like lead, chromium, and nickel. Bioleaching extracts metals from sewage sludge using bacteria at low pH and high iron concentrations over 4-10 days, increasing removal of chromium, copper, and zinc. Extracellular polymeric substances from Cloacibacterium normanense removed 85% nickel and 72% aluminum from wastewater.
Green synthesis and characterization of silver nanoparticles using tinosopora...IJARIIT
The document summarizes research on the green synthesis of silver nanoparticles using an extract of Tinospora cordifolia and their characterization and antimicrobial activity. Key findings include:
- Silver nanoparticles ranging from 27-58 nm were successfully synthesized using a T. cordifolia extract, as confirmed by UV-Vis and SEM analysis.
- XRD and FTIR analysis revealed the nanoparticles were crystalline in nature and that biomolecules in the plant extract were responsible for silver ion reduction and nanoparticle stabilization.
- The synthesized silver nanoparticles demonstrated antimicrobial activity against several pathogenic bacteria, indicating potential applications in medicine, food, and cosmetics.
synthesis of nanoparticles by green chemistry approach and their anticancer a...Gulzar Ahmed Rather
1) Klebsiella pneumonia was used to biologically synthesize gold nanoparticles, as indicated by a color change from white to yellow to dark purple.
2) The gold nanoparticles were characterized using UV-visible spectroscopy, FE-SEM, and XRD, and were spherical in shape with sizes ranging from 40-60nm.
3) The biologically synthesized gold nanoparticles showed antibacterial activity against several human pathogens, including Staphylococcus aureus and Escherichia coli, as measured using the disc diffusion method.
This PowerPoint is one small part of the Taxonomy and Classification unit from www.sciencepowerpoint.com. A 3800+ slide Five Part PowerPoint presentation becomes the roadmap for an amazing and interactive science experience full of built-in lab activities, built-in quizzes, video links, class notes(red slides),review games, projects, unit notes, answer keys, and much more. Also included is a student version of the unit that is much like the teachers but missing the answer keys, quizzes, PowerPoint review games, hidden box challenges, owl, and surprises meant for the classroom. This is a great resource to distribute to your students and support professionals. The Classification and Taxonomy Unit covers topics associated with Taxonomy and Classification. The unit examines all of the Kingdoms of Life in detail. Areas of Focus within The Taxonomy and Classification Unit: -Taxonomy, Classification, Need for Taxonomy vs. Common Names, What is a Species?, Dichotomous Keys, What does Classification Use?, The Domains of Life, Kingdoms of Life,The 8 Taxonomic Ranks, Humans Taxonomic Classification, Kingdom Monera, Prokaryotic Cells, Types of Eubacteria, Bacteria Classification, Gram Staining,Bacterial Food Borne Illnesses, Penicillin and Antiseptic, Oral Hygiene and Plaque, Bacterial Reproduction (Binary Fission), Asexual Reproduction, Positives and Negatives of Bacteria, Protista, Plant-like Protists, Animal-like Protists, Fungi-like Protists, Animalia, Characteristics of Animalia, Animal Symmetry, Phylums of Animalia (Extensive), Classes of Chordata, Mammals, Subclasses of Mammals, Characteristics of Mammals, Fungi, Positives and Negatives of Fungi, Divisions of Fungi (Extensive), Parts of a Mushroom, 3 Roles of Fungi, Fungi Reproduction, Mold Prevention, Plant Divisions, Kingdom Plantae. If you have any questions please feel free to contact me. Thanks again and best wishes. Sincerely, Ryan Murphy www.sciencepowerpoint@gmail.com
This document provides an overview of fungal infections (mycoses) including definitions, classifications, pathogenesis, clinical manifestations, laboratory diagnosis and examples. It discusses superficial mycoses including pityriasis versicolor caused by Malassezia furfur. It also discusses cutaneous mycoses such as dermatophytosis caused by fungi like Trichophyton, Epidermatophyton and Microsporum. Deep mycoses caused by fungi that infect internal organs are also described, including histoplasmosis caused by Histoplasma capsulatum, cryptococcosis caused by Cryptococcus neoformans, aspergillosis caused by Aspergillus fumigatus, mucormycosis
The document discusses various opportunistic mycoses caused by yeasts and molds. It describes the classification of organisms that cause opportunistic mycoses like Candida, Aspergillus, and Zygomycetes. It provides details on diseases caused, host factors, clinical manifestations, laboratory diagnosis and treatment of common fungal infections including candidiasis, aspergillosis, and mucormycosis.
Southern Blotting (SB) 4 jan 2015 finalICHHA PURAK
The power Point presentation contains 38 slides explaining about different steps involved in Southern Blotting such as DNA Isolation, Restriction digestion, Separation of DNA fragments by gel electrophoresis, denaturation of Double stranded DNA , transfer of fragments from gel to membrane ( blotting) , hybridization and detection by autoradiography. Applications of Southern blotting have also been discussed
This presentation discusses the use of special stains in histopathology. It begins by introducing routine H&E staining and then describes several common special stains used to diagnose renal diseases, including PAS, silver, and trichrome stains. Specific examples are given of how these stains can highlight different structures in renal conditions like membranous glomerulonephritis, focal segmental glomerulosclerosis, and amyloidosis. The goal of special stains is to provide additional diagnostic information beyond routine H&E.
SDS-PAGE is a standard technique used to separate proteins based on their molecular weight. It involves coating proteins with SDS detergent to linearize them, then running them through a polyacrylamide gel with an electric current which causes smaller proteins to migrate faster, allowing separation based on size. The document provides details on the principle, gel preparation, running the electrophoresis, visualizing results with stains like Coomassie blue or silver stain, and applications like determining protein purity or identification.
The document discusses the structure and formation of proteins. It explains that amino acids join through condensation reactions to form peptide bonds, releasing a water molecule. Multiple peptide bonds form polypeptide chains of varying lengths that fold into unique 3D shapes to serve as functional proteins, such as structural, storage, transport, hormonal, receptor, contractile, defensive, and enzymatic proteins. Common food sources of protein are also mentioned.
The document discusses the levels of protein structure from primary to quaternary structure. It defines the primary structure as the amino acid sequence. Secondary structure forms from hydrogen bonding between amino acids and includes alpha helices and beta pleated sheets. Tertiary structure results from folding influenced by interactions between amino acid side chains. Quaternary structure occurs when multiple polypeptide chains interact to form a protein complex. Examples including hemoglobin and glyceraldehyde-3-phosphate dehydrogenase are provided to illustrate the different levels of structure.
This document discusses the different levels of protein structure: primary, secondary, tertiary, and quaternary. The primary structure refers to the amino acid sequence. Secondary structure includes alpha helices, beta sheets, and beta turns formed by hydrogen bonding between amino acids. Tertiary structure is the 3D conformation determined by interactions between side chains. Quaternary structure refers to the arrangement of multiple polypeptide subunits in multimeric proteins. The structures are determined through techniques like X-ray crystallography and NMR.
Proteins are composed of amino acids and play many essential roles in the body. They have four levels of structure: primary, secondary, tertiary, and quaternary. The primary structure is the amino acid sequence, secondary involves hydrogen bonding into shapes like alpha helices and beta sheets, tertiary is the 3D folding of these structures, and quaternary involves the assembly of multiple protein subunits. Proteins serve as enzymes, hormones, antibodies, and structures. They undergo synthesis from amino acids and breakdown through catabolism. Disorders can occur if amino acid metabolism is disrupted.
The document provides information on the structure of DNA and RNA. It discusses how DNA was discovered to have a double helix structure by Watson and Crick in 1953 based on prior work by scientists like Franklin, Wilkins, Chargaff and Pauling. It describes the key components of DNA including the sugar-phosphate backbone, nitrogenous bases, and how the bases pair up in the double helix structure. It also discusses different DNA structures like A, B and Z-DNA and how DNA packages into nucleosomes and chromosomes. For RNA, it notes that it is similar to DNA but contains the sugar ribose and base uracil instead of thymine.
Blotting techniques includes southren,northern,western and dot blottingbbmy
This document describes various blotting techniques used to detect specific DNA or RNA sequences, including Southern blotting, Northern blotting, Western blotting, and dot blotting. Southern blotting involves transferring DNA fragments separated by gel electrophoresis to a membrane and probing for specific sequences. Northern blotting is similar but uses RNA. Western blotting detects specific proteins. Dot blotting detects sequences in non-fractionated samples by directly applying samples to a membrane. These techniques allow for detection and analysis of genetic material.
Ion exchange chromatography uses charged sites on a stationary phase to selectively retain ionized solutes from a mobile phase based on electrostatic attraction. Cation exchangers contain negatively charged groups that attract positively charged cations, while anion exchangers contain positively charged groups that attract negatively charged anions. Key factors that influence selectivity include ion charge, hydrated radius, and polarizability. Ion exchange chromatography has various applications including separation of ions, removal of interferents, water softening, and demineralization.
This document summarizes a study on developing a silver/chitosan bionanocomposite using the extract of the Peepal tree (Ficus religiosa) for combating infections associated with biomedical implants. Silver nanoparticles were biosynthesized using the plant extract and characterized using UV-Vis spectroscopy and TEM. The nanoparticles were then incorporated into a chitosan matrix to form a bionanocomposite. This composite was tested as a coating on stainless steel implants to provide antimicrobial properties and reduce biomaterial-associated infections. The authors believe this to be the first example of mythology (the Peepal tree) converging with nanotechnology for a biomedical application.
This document presents a layout for a presentation on synthesizing and characterizing iron oxide nanoparticles. The presentation aims to develop an alternative green method for synthesizing iron oxide nanoparticles from locally available tea leaves and to investigate their antibacterial and antifungal properties. The experimental section describes synthesizing iron oxide nanoparticles from tea leaf extract and coating them with chitosan. Characterization techniques like SEM, XRD, and FTIR are used to analyze the nanoparticles. Antibacterial tests on E. coli and antifungal tests on Candida albicans show the coated nanoparticles have antibacterial and antifungal properties. The expected outcome is that the green synthesis method could be used to develop novel biomaterials and the nanoparticles could help treat
Biosynthesis of Silver Nanoparticles using Plants and its Application on the ...IRJET Journal
The document summarizes research on using silver nanoparticles synthesized from various plant extracts (neem, mango, lemon, and a combination) to treat textile mill wastewater. Key points:
1. Silver nanoparticles were synthesized using extracts from different plants and characterized using SEM.
2. These silver nanoparticles were then used to treat textile mill wastewater and effectively removed 97-93% color and 49-43% COD within 15-60 minutes, with neem-synthesized nanoparticles performing best.
3. Various isotherm and kinetic models were applied to understand adsorption, with Freundlich isotherm and pseudo-second order kinetics providing best correlation. Dubinin-Radush
Antimicrobial activity of silver nanoparticles from capsicum sp. against stap...Alexander Decker
This document summarizes a study that synthesized silver nanoparticles from Capsicum sp. (pepper) extract and tested their antimicrobial activity against various pathogens. Key findings:
- Pepper extract was used to reduce silver ions and synthesize silver nanoparticles, confirmed by a UV-Vis absorption peak at 480nm.
- Disk diffusion tests showed the silver nanoparticles had concentration-dependent antibacterial activity against Staphylococcus, Bacillus, E. coli, and Pseudomonas. Higher concentrations inhibited more growth.
- The nanoparticles were most effective against Pseudomonas, followed by Bacillus, Staphylococcus, and E. coli, showing activity against both gram-positive and gram-negative bacteria.
This document describes a study on using silver nanoparticles incorporated onto polyurethane foam to mineralize pesticides in water. Silver nanoparticles were synthesized using trisodium citrate and characterized before being incorporated onto polyurethane foam. The foam was then used to mineralize chlorpyrifos and malathion in water solutions at different concentrations over time. Mineralization time was found to increase with higher initial pesticide concentration, with chlorpyrifos being fully mineralized faster than malathion at the same concentrations. The study evaluated silver nanoparticles on polyurethane foam as a potential method for removing pesticides from contaminated water.
green synthesis of metal and their oxide nanoparticles-2.pptxmuhammadhaini99
Title: Green Synthesis of Metal and Metal Oxide Nanoparticles: A Sustainable Approach
Abstract:
In recent years, there has been a growing interest in the green synthesis of nanoparticles, particularly metal and metal oxide nanoparticles, due to their wide range of applications and the increasing need for sustainable production methods. Green synthesis offers an environmentally friendly alternative to traditional chemical synthesis routes by utilizing natural extracts, biomolecules, or other eco-friendly materials as reducing and stabilizing agents. This paper provides an in-depth exploration of green synthesis methods for the production of metal and metal oxide nanoparticles, highlighting their advantages, mechanisms, and applications. Through a comprehensive review of the literature, various green synthesis approaches, including plant-mediated, microbial, and bio-inspired methods, are discussed. The properties and characterization techniques of green-synthesized nanoparticles are also examined, along with their potential applications in catalysis, sensing, drug delivery, and environmental remediation. Overall, this review underscores the importance of green synthesis as a sustainable approach to nanoparticle production and its significant implications for both scientific research and industrial applications.
Keywords: Green synthesis, Metal nanoparticles, Metal oxide nanoparticles, Sustainable production, Catalysis, Sensing, Drug delivery, Environmental remediation.
Introduction
The synthesis of nanoparticles has gained considerable attention in recent years due to their unique physical, chemical, and biological properties, which differ from those of their bulk counterparts. These properties make nanoparticles promising candidates for various applications in fields such as catalysis, electronics, medicine, and environmental remediation. However, traditional methods of nanoparticle synthesis often involve the use of toxic chemicals, high temperatures, and energy-intensive processes, leading to environmental pollution and health hazards. In response to these challenges, there has been a growing interest in developing sustainable and environmentally friendly approaches to nanoparticle synthesis, known as green synthesis.
Green synthesis involves the use of natural extracts, biomolecules, or other eco-friendly materials as reducing and stabilizing agents in nanoparticle synthesis. This approach offers several advantages over conventional synthesis methods, including reduced environmental impact, cost-effectiveness, scalability, and the ability to produce nanoparticles with controlled size, shape, and composition. Among the various types of nanoparticles, metal and metal oxide nanoparticles have received significant attention due to their diverse applications and potential for green synthesis. In this paper, we provide a comprehensive review of green synthesis methods for the production of metal and metal oxide nanoparticles, highlighting their
Synthesis and evaluation of bactericidal properties of CuO nanoparticles agai...Nanomedicine Journal (NMJ)
Objective(s):
CuO is one of the most important transition metal oxides due to its captivating properties. It is used in various technological applications such as high critical temperature superconductors, gas sensors, in photoconductive applications, and so on. Recently, it has been used as an antimicrobial agent against various bacterial species.
Materials and Methods:
Here, we synthesized CuO nanoparticles (NPs) and explored the antibacterial activity of CuO NPs preparation.
Results:
Single crystalline nanoparticles of copper oxide having almost uniform particle size of 5-6 nm has been synthesized by a facile and versatile route. XRD spectra confirmed the formation of single phase CuO NPs. Transmission electron microscopy results corroborate well with XRD results. The technique employed is free from toxic solvents, organics and amines, is based on a simple reaction of copper sulfate and de-ionized water (DI), and their bactericidal effects against of Aeromonas hydrophila ATCC 7966T bacteria were investigated. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) with liquid culture for all of the Aeromonas hydrophila culture Medias was done.
Conclusion:
Present study confirms that Copper oxide nanoparticles have great promise as antimicrobial agent against Aeromonas hydrophila.
Silver Nanoparticles from a Plant Echinacea Purpurea Extract for its Antipath...ijtsrd
This document summarizes a study on the green synthesis of silver nanoparticles using a plant extract from Echinacea purpurea and evaluating their antibacterial properties. Key findings include:
- Silver nanoparticles were successfully synthesized using an extract of E. purpurea, as confirmed by a color change and characterization using UV-Vis spectroscopy and SEM analysis.
- The silver nanoparticles showed antibacterial effects against several pathogens like E. coli and K. pneumoniae, as demonstrated through spread plate, pour plate, and disc diffusion assays. Higher concentrations of nanoparticles resulted in fewer bacterial colonies.
- The mechanism of antibacterial action may involve nanoparticles binding to bacterial membranes and releasing silver ions, though more study is needed to understand
Removal of Lead Ion Using Maize Cob as a BioadsorbentIJERA Editor
The intensification of industrial activity and environmental stress greatly contributes to the significant rise of
heavy metal pollution in water resources making threats on terrestrial and aquatic life. The toxicity of metal
pollution is slow and interminable, as these metal ions are non bio-degradable. The most appropriate solution for
controlling the biogeochemistry of metal contaminants is sorption technique, to produce high quality treated
effluents from polluted wastewater. Maize cob readily available was used as sorbent for the removal of lead ions
from aqueous media. Adsorption studies were performed by batch experiments as a function of process
parameters such as sorption 500ppm,2.5g, 400minutes, 400 rpm and 5 PH. Concentration, Dosage, time, rpm,
and pH. I have found that the optimized parameters are Freundlich model fits best with the experimental
equilibrium data among the three tested adsorption isotherm models. The kinetic data correlated well with the
Lagergren first order kinetic model for the adsorption studies of lead using maize cob. It was concluded that
adsorbent prepared from maize cob as to be a favorable adsorbent and easily available to remove the heavy
metal lead (II) is 95 % and can be used for the treatment of heavy metals in wastewater.
Nanoparticles Methods for Nanoparticles Synthesis Overviewijtsrd
Nanoparticles exist in several different morphologies such as spheres, cylinders, platelets, tubes etc. The word nanoparticles are used to describe a particle with size in the range of 1nm to 100nm, at least in one of the three possible dimensions. In this size range, the physical, chemical and biological properties of the nanoparticles changes in fundamental ways from the properties of both individual atoms molecules and of the corresponding bulk materials. The enormous diversity of the nanoparticles arising from their wide chemical nature, shape and morphologies, the medium in which the particles are present, the state of dispersion of the particles and most importantly, the numerous possible surface modifications the nanoparticles can be subjected to make this an important active field of science now a days. Dr. Ilamathi Jayaraman | Dr. Vijayakumari. S "Nanoparticles: Methods for Nanoparticles Synthesis: Overview" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46478.pdf Paper URL : https://www.ijtsrd.com/biological-science/biotechnology/46478/nanoparticles-methods-for-nanoparticles-synthesis-overview/dr-ilamathi-jayaraman
Study of Biocidal Activity of Copper A Reviewijtsrd
Copper ions, either alone or in copper complexes, have been used to disinfect liquids, solids and human tissue for centuries. Today copper is used as a water purifier, algaecide, fungicide, nematocide, molluscicide as well as an anti bacterial and anti fouling agent. Copper also displays potent anti viral activity. We have explained i the biocidal properties of copper ii the possible mechanisms by which copper is toxic to microorganisms and iii the systems by which many microorganisms resist high concentrations of heavy metals, with an emphasis on copper. Health care associated infections HAIs are a global problem associated with significant morbidity and mortality. Controlling the spread of antimicrobial resistant bacteria is a major public health challenge, and antimicrobial resistance has become one of the most important global problems in current times. The antimicrobial effect of copper has been known for centuries, and ongoing research is being conducted on the use of copper coated hard and soft surfaces for reduction of microbial contamination and, subsequently, reduction of HAIs. Dr. Pragya Tank "Study of Biocidal Activity of Copper: A Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-5 , August 2022, URL: https://www.ijtsrd.com/papers/ijtsrd50696.pdf Paper URL: https://www.ijtsrd.com/chemistry/other/50696/study-of-biocidal-activity-of-copper-a-review/dr-pragya-tank
Mass Transfer, Kinetic, Equilibrium, and Thermodynamic Study on Removal of Di...Ratnakaram Venkata Nadh
Three distinct agricultural waste materials, viz., casuarina fruit powder (CFP), sorghum stem powder
(SSP), and banana stem powder (BSP) were used as low-cost adsorbents for the removal of toxic lead(II)
from aqueous solutions. Acid treated adsorbents were characterized by scanning electron microscopy (SEM),
energy-dispersive X-ray spectroscopy (EDX), and Fourier transform infrared spectroscopy (FTIR). The
effects of parameters like adsorbent dose, pH, temperature, initial metal ion concentration, and time of
adsorption on the removal of Pb(II) were analyzed for each adsorbent individually and the efficiency order
was BSP > SSP > CFP. Based on the extent of compatibility to Freundlich/Langmuir/Dubinin–Radushkevich/
Temkin adsorption isotherms and different models (pseudo-first and second order, Boyd, Weber’s, and
Elovich), chemisorption primarily involved in the case of BSP and SSP, whereas simultaneous occurrence of
chemisorption and physisorption was proposed in the case of CFP correlating with the thermodynamic study
results conducted at different temperatures. Based on the observations, it was proposed that three kinetic
stages involve in the adsorption process, viz., diffusion of sorbate to sorbent, intra particle diffusion, and then
establishment of equilibrium. These adsorbents have a promising role towards the removal of Pb(II) from
industrial wastewater to contribute environmental protection
This document provides information about the phytosynthesis of metal nanoparticles. It begins with an introduction to nanoparticles and different synthesis methods. The document then describes using plant extracts to biologically synthesize nanoparticles through reduction of metal ions. It provides details on characterization techniques for nanoparticles, such as UV-vis spectroscopy, XRD, TEM, and FTIR. The document also gives an example of using Pongamia pinnata leaf extract to synthesize silver nanoparticles and characterize them.
Evaluation of Cadmium tolerant Fungi in the dying Staff and their removal Pot...AI Publications
Cadmium tolerance and bioremediation capacity of seven isolates including Aspergilus versicolor, Aspergillus fumigatus, Paecilomyces sp.9, Paecilomyces sp.G, Terichoderma sp, Microsporum sp,Cladosporium sp were determined. Minimum inhibitory concentration values among 2,000-6,000 mg lˉ1proved great ability of isolated strains to survive in cadmium polluted environments. The most tolerant fungi, Aspergilus versicolor, showed tolerance index of 0.93 in 100 mg lˉ1 cadmium agar media. Fungal resistance against cadmium is depended directly on strain’s biological function. A. versicolor was found to bioaccumulation over 7.67mg of cadmium per 1 g of mycelium, followed by 6.546, 6.354, and 6.286, 6.134 by Paecilomyces sp, Aspergilus fumigatus, Microsporum sp and Terichoderma sp, respectively. It can be noted that tolerance of the strains appears to be independent from bioaccumulation capacity. Finally, the results indicated that A. versicolor could be a prospective candidate for bioremediation processes.
This document discusses the synthesis and pharmacological aspects of silver nanoparticles (SNPs). It describes how SNPs can be synthesized using traditional Ayurvedic methods, physicochemical methods such as chemical reduction and photochemical processes, and biological methods involving bacteria, fungi, and plant extracts. Physicochemical methods are widely used but biological methods provide environmentally friendly alternatives. The document also reviews the reported antimicrobial properties and medical applications of SNPs, noting their effectiveness against bacteria, viruses, and fungi makes them promising for use against various infectious diseases. However, more research is still needed to fully understand the pharmacological potential of SNPs.
Biosorption of Copper (II) Ions by Eclipta Alba Leaf Powder from Aqueous Solu...ijtsrd
The removal of heavy metals from industrial wastewater is of great concern as heavy metals are non-biodegradable, toxic elements that cause serious health problems if disposed of in the surrounding environment. The present study, Karisalangkani (Eclipta Alba) leaves were used for the adsorption of heavy metals like copper (Cu (II)) ions. The bio sorbent was characterized using SEM and BET analysis. The bio sorption experiments are conducted through batch system. The operating parameters studied were initial metal ion concentration, adsorbent dosage, initial solution pH, contact time and effect of temperature Adsorption equilibrium is achieved in 30 min and the adsorption kinetics of Cu (II) is found to follow a pseudo-second-order kinetic model. Equilibrium data for Cu (II) adsorption are fitted well by Langmuir isotherm model. The maximum adsorption capacity for Cu (II) ions is estimated to be 9.2 mgg at 25 °C. The experimental result shows that the materials have good potential to remove heavy metals from effluent and good potential as an alternate low cost adsorbent. Due to their outstanding adsorption capacities, Eclipta Alba is excellent sorbents for the removal of copper (II) ions. B. Kavitha | R. Arunadevi"Biosorption of Copper (II) Ions by Eclipta Alba Leaf Powder from Aqueous Solutions" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-5 , August 2018, URL: http://www.ijtsrd.com/papers/ijtsrd17156.pdf http://www.ijtsrd.com/chemistry/environmental-chemistry/17156/biosorption-of-copper-ii-ions-by-eclipta-alba-leaf-powder-from-aqueous-solutions/b-kavitha
Invitro Assessment of Antibacterial and Antioxidant Property of Biofabricated...ijtsrd
Biosynthesis of silver nanoparticles is an eye catching area of a modern research field. It is simple, single step, non toxic and eco friendly method. In the present investigation, the aqueous extract of Bauhinia tomentosa is used to synthesise silver nanoparticles and it was confirmed by UV Visible spectrometer. Due to surface Plasmon resonance, the peak obtained at 440.0 nm. The functional group present at the surface of the silver nanoparticles was confirmed by FT IR analysis. Further, the synthesized silver nanoparticles were subjected to antibacterial activity by Well diffusion method and also antioxidant activity performed by hydrogen peroxide scavenging assay. As concentration of silver nanoparticles increases the bactericidal activity is raised and also the antioxidant activity is increased. Thus it reveals that due to presence of various bioactive compounds present in the plant extract acting as reducing agent to reduce the size of silver ions into silver nanoparticles. Therefore, the biosynthesized nanoparticles is utilized for various biomedical applications. Mohanapriya. P ""Invitro Assessment of Antibacterial and Antioxidant Property of Biofabricated Silver Nanoparticles using Aqueous Extract of Bauhinia Tomentosa"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd22780.pdf
Paper URL: https://www.ijtsrd.com/engineering/nano-technology-/22780/invitro-assessment-of-antibacterial-and-antioxidant-property-of-biofabricated-silver-nanoparticles-using-aqueous-extract-of-bauhinia-tomentosa/mohanapriya-p
This document summarizes a student project on the green synthesis of nanoparticles. It discusses various methods for synthesizing nanoparticles, emphasizing that green synthesis is more eco-friendly than physical or chemical methods as it does not require high temperatures, pressures, or toxic chemicals. The document then describes how plant extracts can be used to synthesize nanoparticles and the characterization techniques used to analyze the particles produced, including UV-vis spectroscopy, DLS, SEM, TEM and FTIR. It concludes by noting some applications of green-synthesized nanoparticles in fields such as medicine, environment and engineering.
Combined effects of copper and cadmium on Chlorella pyrenoidosa H.Chick: Subc...UniversitasGadjahMada
Disposal of waste into aquatic ecosystems may cause microalgae to be exposed to various metals, e.g. copper and cadmium. The effects caused by combinations of metals may be more serious. Evaluations of subcellular fate, bioaccumulation, and biological effects of metals on aquatic organisms are generally derived from experiments with individual metals. The present study aims to evaluate the effects of exposure of Chlorella pyrenoidosa to copper and cadmium combined on subcellular accumulation, distribution, and growth. The algae were exposed for 72 h to copper at concentrations of 13 – 25 μmol L-1, cadmium at about 6 μmol L-1, and combinations thereof. The levels of copper and cadmium in subcellular organelles, heatdenaturated protein, metal-rich granules, and heat-stable protein were determined by atomic absorption spectrometry. Exposure of C. pyrenoidosa to copper and cadmium in combination inhibited growth more strongly than copper and cadmium individually. Highest accumulation was observed in metal-rich granules and heat-stable proteins. Administration of both metals in combination affected their subcellular distribution: copper was mainly distributed into the metal-rich granules (70 - 80 %) and heat-stable proteins (9 - 24 %), cadmium in the metal-rich granules (88 - 98 %).
This document summarizes a study on using chitin biopolymer blended with bentonite clay to remove copper from wastewater via adsorption. Characterization of the blended material was done using FTIR, XRD, TGA and SEM. FTIR showed interactions between chitin and bentonite through hydrogen bonding. XRD indicated the blend was amorphous. TGA showed the blended material had higher thermal stability than chitin alone. SEM revealed the blend had a porous structure and large surface area suitable for adsorption. Batch adsorption studies showed the blended biopolymer effectively removed copper from wastewater.
UiPath Test Automation using UiPath Test Suite series, part 6DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 6. In this session, we will cover Test Automation with generative AI and Open AI.
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Jurnal membran 140210110088
1. ArchiveofSID
Iranian Polymer Journal
18 (5), 2009, 383-392
silver nano-sized particles;
chitosan;
chitosan membrane;
wood staining fungi;
antifungal activity.
(*) To whom correspondence to be addressed.
E-mail: ysoolee@chonbuk.ac.kr
A B S T R A C T
Key Words:
Synthesis and Characterization of Potential
Fungicidal Silver Nano-sized Particles and Chitosan
Membrane Containing Silver Particles
Natarajan Velmurugan1, G Gnana Kumar2, Sang Sub Han3,
Kee Suk Nahm2, and Yang Soo Lee1,3*
(1) Department of Forest Science & Technology, Institute of Agriculture and Life
Sciences; (2) Specialized Graduate School of Hydrogen and Fuel Cell Engineering;
(3) Institute of Agricultural Science & Technology, Chonbuk National University,
Chonju, Chonbuk 561-756, Republic of Korea
Received 19 November 2008; accepted 13 April 2009
S
ilver nano-sized particles were synthesized with strong antifungal activity against
the wood staining fungi Ophiostoma flexuosum, O. tetropii, O. polonicum and O.
ips. The average size and purity of the nano-sized particles were determined by
scanning electron microscopy and XRD spectra, respectively. The structural character-
izations of nano-sized particles were performed using IR spectrum and EDS. Radial
diffusion method was carried out to reveal the antifungal activity of silver nano-sized
particles on MEA agar plates. Strong fungicidal silver nano-sized particles were mixed
with chitosan solution and then the chitosan membrane was prepared by solvent
evaporation method. Glutaraldehyde was used as cross-linking agent for membrane
preparation. Deacetylation degree, concentration and pH of chitosan were considered
as a main factor for preparation of bioactive membrane. The quality and structure of
membranes were analyzed by Fourier transform infrared spectroscopy. Thermostability
of the membranes was confirmed by thermogravimetric analysis. Synthesized
chitosan-glutaraldehyde-Ag membrane was tested against the wood staining fungi
on MEA agar plates using inhibition zone method. The results showed that the antifun-
gal activity of chitosan membrane increased with increasing in concentration of silver
nano-sized particles. According to our results, synthesized chitosan membrane has
been used as a good carrier for silver nano-sized particles in wood industries and these
materials could be very efficient substitutions for chemical preservatives currently used
to control fungal contamination in wood industries.
INTRODUCTION
Chitosan is an environmental
friendly, nontoxic polymer derived
by partial deacetylation of chitin.
Many researchers synthesized
chitosan membrane using different
solvents with various properties, to
use in various fields, such as thera-
peutic formulations, drug delivery,
food preservation and package,
waste materials treatment, bio-
degradable products production
and separation processes [1-6].
Chitosan, a polymer composed of
β-(1 4)-N-acetyl-D-glucosamine,
a major component of crab, shrimp
and crawfish [7]. The antimicrobial
and antifungal responsibility of
chitosan depends on the following
reasons: chitosan deacetylation
degree, molecular weight, pH of
the medium and temperature [8].
Many authors have reported the
Available online at: http://journal.ippi.ac.ir
www.SID.ir
2. potential antimicrobial activity of chitosan [4,8], yet
the exact mechanism of antimicrobial activity of
chitosan is not studied well. Among many reasons
suggested by researchers it is mainly explained by the
chelating character of chitosan, capability to modify
the concentrations of Ca2+ ions and other essential
minerals, trace elements important for microbial
growth especially for filamentous fungal growth.
Some other mechanisms have also been proposed
towards the antibacterial and antifungal activity of
chitosan; the antifungal inhibition could be due to the
direct interaction between cell membrane and
chitosan, as chitosan can directly disrupt the mem-
brane function and the pH of chitosan plays crucial
role in the interaction between cell protein and chi-
tosan. At acidic pH the interaction between chitosan
and proteins is low, and accordingly most proteins
can be expected to be below their isoelectric point
and positively charged at acidic pH [9]. Chitosan can
also be inhibiting the cell transcription and translation
indirectly by interaction of hydrolysis products with
bacterial and fungal DNA, which prevents the
synthesis of mRNA and proteins [8].
Silver nanoparticles are widely known for its
bactericidal and fungicidal activity. Silver nanoparti-
cles are very effective antimicrobial and antifungal
agents at lower concentration [10]. The antimicrobial
activity of silver is much higher than other metals,
such as mercury, copper, lead, chromium and tin.
Silver (I) oxide can produce free silver ions in the
presence of oxygen, thus, the antimicrobial activity of
silver depends on the active surface of silver. Release
of free silver ions from silver (I) oxide by chitosan is
more quick and rapid [8]. At lower concentration,
silver nanoparticles directly damage the cell envelope
by penetrating the cell and then silver binds to the
DNA, this complex prevents the DNA replication by
displacement of hydrogen bonds between adjacent
nitrogens of purines and pyrimidines [11]. Chitosan
has a high metal binding potential, especially for
silver [4].
The amine and hydroxyl groups of chitosan play
important role in uptake of silver cations by a chela-
tion mechanism and sorption, respectively. Binding
of silver nanoparticles into chitosan membrane main-
ly depends on the pH. Large quantity of silver
nanopartilces binds with chitosan membrane at high-
er pH than lower pH, since the amino group is proto-
nated at lower pH [4].
Commercial usages of chitosan membrane have
been reported widely in the literature, especially used
in food industries. Chitosan used to improve shelf-life
period of bread, kimchi (Korean traditional vegetable
fermented food), milk, noodle, rice cake, soybean
curd, soybean sprouts, and starch jelly. Chitosan used
as protective barriers for egg, fruits, and vegetables
storage and processing aid in fruit juice production.
Spoilage bacterial growth inhibited by chitosan in
seafood’s and seafood products by retarding the lipid
oxidation [7]. Chitosan coating and films are used in
storage of perishable foods [7].
According to recent findings, feasibility of silver
nano-sized particles as antibacterial agent is well
established while a very few investigations have been
carried out on possible usage of silver nano-sized par-
ticles and chitosan as a favourite wood preservative
material [6,12]. The effective role of silver nano-
sized particles and chitosan against wood staining
fungi is not yet been established well. The most
important goal of this work is to synthesize the silver
nano-sized particles with very low minimum
inhibitory concentration (MIC) against wood staining
fungus which validate its application in wood indus-
tries and based on our knowledge, this is the first
attempt to evaluate the antifungal activity of chitosan
(as a membrane form) against wood contaminating
fungi. The aim of our research work is to prepare chi-
tosan membrane containing silver nano-sized parti-
cles with strong antifungal activity against wood
staining fungi. Chitosan membrane was prepared by
mixing appropriate concentrations of chitosan, silver
nano-sized particles and glutaraldehyde by magnetic
stirring, the synthesized membrane was characterized
and then tested against wood staining fungi in in vitro
condition. This research work could be helpful to use
the silver nano-sized particles and chitosan as a wood
preservative material in wood industries.
EXPERIMENTAL
Fungal Strains and Materials
The following sapstaining fungal samples were used
to determine the antifungal activity of silver nano-
Synthesis and Characterization of Potential Fungicidal ... Velmurugan N et al.
Iranian Polymer Journal / Volume 18 Number 5 (2009)384
www.SID.ir
3. particles and membrane: Ophiostoma flexuosum
(363175), Ophiostoma tetropii (363182), Ophiostoma
polonicum (343181) and Ophiostoma ips (363176).
All the fungal cultures were obtained from CABI,
Bioscience, UK Centre, formerly called as
International Mycological Institute (IMI). The fungal
cultures were grown in 2% MEA (Becton, Dickinson
and Company, USA) medium as pre-inocula at 25ºC
for 4-7 days.
Chitosan (MW 100,000, 80% deacetylation
degree) was obtained from Textile Chemistry
Laboratory, Chonbuk National University, S. Korea.
Analytical grade chemicals glutaraldehyde, iso-
propanol, hydrochloric acid, sodium borohydride, and
silver nitrate were obtained from Sigma-Aldrich,
USA.
Silver Nano-sized Particles Synthesis
Silver nitrate, sodium borohydride, distilled water
were used as source materials for preparation of silver
nanoparticles. Analytically pure grade chemicals were
used for this experiment. 0.003 M of hydrated silver
nitrate solution and 0.002 M of sodium borohydride
solutions were prepared and chilled to 0ºC, separate-
ly. The chilled silver nitrate solution was added drop
by drop into the sodium borohydride solution which
was taken in a reservoir under nitrogen atmosphere
and stirred for 2 h. After stirring, silver ions were
reduced to form the monodispersed nanoparticles in
the solution. Finally, nanoparticles solution was
stored at room temperature.
Membrane Preparation
At first, 2% chitosan solution was sterilized at 121ºC
for 20 min and the chitosan membranes were devel-
oped by solvent evaporation and film casting tech-
niques. The appropriate amounts of the sterilized
chitosan (chitosan solution was maintained with pH
5), silver nano-sized particles (10 ppm, 50 ppm and
100 ppm) and 5 vol% of glutaraldehyde solution
which consists of IPA/water (90/10 vol%) mixture, 1
vol% of hydrochloric acid as a catalyst were mixed
well and magnetically stirred for 2-3 min. The result-
ant viscous solution was spread on a clean glass plate
and dried at 37ºC for 48 h. Silver nano-sized particles
were sonicated for 10 min before addition with
chitosan mixture.
Characterization of Silver Nano-sized Particles
and Membrane
Morphological characteristics of the synthesized
nano-sized particles and mapping were analyzed by
scanning electron microscope coupled with EDX and
elements mapping. Air-dried samples were used for
SEM analysis. The samples were coated with gold by
ion sputtering (Jeol JFC-1200 fine coater) and
observed under SEM (JSM-5200). X-Ray diffraction
(XRD) patterns for the silver nano-sized particles
were obtained using a (D-Max 3A, Rigaku XRD)
diffractometer in the 2θ range from 30 to 80 with Cu,
Kα radiation. The structural characterization of the
silver nanoparticles and membranes were investigated
by FTIR. FTIR spectra of the samples were recorded
at room temperature using Fourier transform infrared
spectroscopy (Spectrum GX, USA) in the region from
400 cm-1 to 3000 cm-1. Thermal behaviour of the
membranes was examined by Perkin Elmer instru-
ment. The TGA measurements were carried out under
a nitrogen atmosphere with a heating rate of 20°C/min
from 30ºC to 800°C.
Antifungal Activity
Evaluation of Antifungal Activity of Silver
Nanoparticles and Chitosan Membrane
Percentage inhibition of sapstaining fungi was meas-
ured by radial mycelial growth method in presence of
various concentrations of silver nano-sized particles.
Four different concentrations (1 ppm, 10 ppm,
50 ppm, and 100 ppm) of silver nano-sized particles
were used in antifungal activity test. Two percentage
MEA media were prepared with above mentioned
percentages of silver nano-sized particles solution,
separately. Six millimeters agar plugs from the pre-
inoculum of O. flexuosum, O. tetropii, O. polonicum
and O. ips were transferred to 2% MEA amended with
different concentrations of the tested mixture. Control
plates for all 4 fungal samples were maintained with-
out antifungal agents. All plates were incubated at
20ºC for 7 days. The observation of mycelial growth
was followed until 7 days and data’s were registered.
Inhibition zone method was carried out to evaluate
the antifungal and antibacterial activity of chitosan
membrane. To make small pieces of membrane, all
membranes were kept in distilled water for 1 h and
membranes were punched to make small pieces
385Iranian Polymer Journal / Volume 18 Number 5 (2009)
Synthesis and Characterization of Potential Fungicidal ...Velmurugan N et al.
www.SID.ir
4. (approximately 6 mm in diameter) and then they were
air-dried inside a laminar air flow chamber. Fungal
samples O. flexuosum, O. tetropii, O. polonicum and
O. ips were inoculated in 2% MEA plates before plac-
ing the membranes. All membrane samples were
placed in 3 corners of each fungus, separately. Plates
were incubated at 20ºC for 7 days.
Statistical Analysis
Additionally, statistical analysis was conducted to
evaluate the fungicidal activity of silver nano-sized
particles. Four replicates were maintained for 4 fun-
gal samples at all concentrations including control.
The difference between the mycelial growths of repli-
cates were not significant, thus the average was used
for antifungal index analysis. Antifungal index was
calculated based on the method of Zhong [13]:
Antifungal index (%) = (1- Dt / Dc) × 100
where Dt = diameter of mycelial growth zone in test
plate; Dc = diameter of mycelial growth zone in con-
trol plate. Results with significant difference P < 0.05
were considered statistically [14].
RESULTS AND DISCUSSION
Characterization of Silver Nano-sized Particles
and Membrane
Figure 1 shows typical SEM image of smooth sur-
face, spherical shape silver nano-sized particles and
Figure 1. SEM image of silver nanoparticles.
Figure 2. EDS profile of silver nanoparticles.
the average size of the nanoparticles found to be 100
to 200 nm. Figure 2 shows the EDS profile of silver
nano-sized particles which indicate the formation of
silver nano-sized particles without any external oxide
contamination. The crystal nature of synthesized
silver nano-sized particles was confirmed by XRD
profile. Four characteristic peaks were appeared for
silver nano-sized particles in XRD profile (Figure 3).
These peaks are exactly matched with the standard
values of metallic silver. XRD and EDS results show
that nanoparticles were produced without any
impurities. Silver nano-sized particles existence was
confirmed by IR spectrum (Figure 4). A strong
band was observed at 2378 cm-1 along with two
other shoulder peaks at 1621 cm-1 and 1318 cm-1.
2378 cm-1 indicates the strong aliphatic C-H
stretching vibration and the additional shoulder peaks
correspond to silver ions.
The thickness differences between the different
Figure 3. XRD pattern of silver nanoparticles.
Iranian Polymer Journal / Volume 18 Number 5 (2009)386
Synthesis and Characterization of Potential Fungicidal ... Velmurugan N et al.
~ 2 μm
www.SID.ir
5. Figure 4. IR spectrum of silver nanoparticles.
membranes were identified by naked visualization.
Pure chitosan membrane was comparatively thinner
than chitosan-glutaraldehyde membrane and chitosan-
glutaraldehyde membrane was darker than chitosan
membrane. It is already reported that thickness of the
membrane depends on long drying time [5]. Thin
membranes were very flexible and transparent than
thick membranes. Thicker membranes were rigid.
Figure 5 displays the FTIR spectra of pure chitosan
membrane and blended chitosan membranes. The
characteristic bands of pure chitosan membrane are
described as follows (Figure 5): the bands observed at
1155, 1067, 1030, and 894 cm-1 are attributed for the
stretching vibration of C–O–C linkages in the saccha-
ride structure. The bands appeared at 1324 cm-1 and
1380 cm-1 reflect the stretching vibration of the C–N
Figure 5. FTIR spectrum of chitosan-glutaraldehyde
membrane.
Figure 6. FTIR spectra of chitosan-glutaraldehyde-Ag
membrane.
bond (amide III) and the C–H binding modes of
methylene. A strong peak observed at 1600 cm-1 is
assigned for the NH2 absorption band [13]. All the
bands described above were observed for the blended
membranes. Addition of silver nitrate shifted the char-
acteristic peak of amide from 1632 cm-1 to 1661 cm-1
and ensures the incorporation of silvers ions into the
membrane (Figure 6). A shift was effectively caused
by the coordination interaction between NH2 groups
of the chitosan and silver ions, called as an inductive
effect [4]. Besides, the homogeneous distribution and
presence of silver nanoparticles in synthesized mem-
brane were confirmed by elements mapping and EDX
analysis (Figures 7 and 8). Carbon peak in EDX may
be attributed to the chitosan in the membrane and Ag
peaks indicate its presence.
Figure 7. Elemental mapping of chitosan-silver membrane.
Synthesis and Characterization of Potential Fungicidal ...Velmurugan N et al.
Iranian Polymer Journal / Volume 18 Number 5 (2009) 387
www.SID.ir
6. Thermostability of the prepared membranes was
confirmed by thermogravimetric analysis (Figure 9).
Weight loss of chitosan and chitosan-glutaraldehyde
membranes were shown in two stages. First stage of
weight loss was observed between 50ºC to 350ºC,
over 50% of weigh loss was observed above 350ºC.
This loss may be due to the loss of adsorbed and
bounded water [15]. A second stage of weight loss
observed between 400ºC to 650ºC, it was attributed to
the degradation of chitosan. A much lower weight loss
was observed for the chitosan-glutaraldehyde mem-
brane than pure chitosan membrane. 60.37% of
weight loss was observed at 650ºC in chitosan-
glutaraldehyde membrane (Table 1), which indicates
the higher thermostable character of chitosan-
glutaraldehyde membrane.
Antifungal Activity of Silver Nano-sized Particles
and Membrane
Wood can easily be infected by a wide range of fungi,
including wood-decay fungi and staining fungi. Wood
staining is caused by sapstaining fungi due to the
production of melanin in ray parenchyma tissues and
cell lumens of fungal hyphae. The strength of wood is
not affected by sapstaining fungi, but it can cause
Figure 8. EDX profile of chitosan-silver membrane.
serious damage in natural colour of wood.
Ophiostoma sp. is one of the major genera of sapstain-
ing fungi. Many authors have studied wood decay
fungi and sapstaining fungi control on wood by chem-
ical, biological agents and natural products. Mycelial
growth of Ophiostoma flexuosum, O. tetropii, O.
polonicum and O. ips were reduced on media amend-
ed with different concentrations of silver nano-sized
particles (Figures 10-13). The results showed that
mycelial inhibition of all tested fungi was started from
minimum concentration of silver nano-sized particles
used in the media. O. flexuosum was highly sensitive
fungus towards silver nanoparticles; no growth was
observed when high concentration (100 ppm) used
(Figure 11). O. ips exhibits slight tolerance against sil-
ver nano-sized particles. Thirty millimeters of O. ips
mycelial growth was observed in MEA medium con-
taining 100 ppm silver nano-sized particles (Figure
10). While 24 mm and 14 mm in diameter mycelial
growth of O. polonicum and O. tetropii were observed
in MEA media containing 100 ppm silver nano-sized
Figure 9. Thermogravimetric analysis of chitosan and
chitosan-glutaraldehyde.
Synthesis and Characterization of Potential Fungicidal ... Velmurugan N et al.
388 Iranian Polymer Journal / Volume 18 Number 5 (2009)
Temperature
(ºC)
Chitosan membrane
weight loss (%)
Chitosan-glutaraldehyde
membrane weight loss (%)
100
200
250
400
650
10.14
22.54
27.99
62.19
70.08
7.12
13.77
25.26
49.50
60.37
Table 1. Thermostability of chitosan and chitosan-glutaraldehyde membrane.
www.SID.ir
7. Figure 10. Effect of silver nanoparticles on mycelial growth
of O. ips.
particles, respectively (Figures 12 and 13).
According to statistical analysis, among four
different concentrations of silver nano-sized particles
solution, the mycelial growth was highly inhibited at
the concentration of 50 ppm and 100 ppm, O. tetropii
was highly sensitive towards all concentrations of sil-
ver nano-sized particles. 56% to 81% of growth
inhibition was observed in O. tetropii plates (Table 2).
While 100 ppm of silver nano-sized particles com-
pletely inhibited the growth of O. flexousum in MEA
agar plates (Table 2). In O. ips plates, 39% and 46%
of growth reduction were observed at 50 ppm and
100 ppm concentration, respectively (Table 2).
Growth inhibition percentage of O. polonicum
increased gradually which was dependent on the
increasing concentration of silver nano-sized particles
39%, 53%, and 68% growth reduction were observed
Figure 11. Effect of silver nanoparticles on mycelial growth
of O. flexousum.
Figure 12. Effect of silver nanoparticles on mycelial growth
of O. polonicum.
at plates amended with 10, 50, and 100 ppm silver
nano-sized particles plates, respectively (Table 2).
Based on our results, the inhibition of radial growth
of all tested fungi was due to the antifungal properties
of silver nano-sized particles.
Chitosan is widely used as a natural product to
control fungal contamination in food industries and
so on. The antifungal activity of chitosan-glutaralde-
hyde-silver nano-sized particles has shown in Figure
8. It is clearly evident that chitosan-glutaraldehyde
membrane containing silver nano-sized particles
showed strong antifungal activity against wood stain-
ing fungi group, Ophiostoma flexuosum, Ophiostoma
tetropii, Ophiostoma polonicum, and Ophiostoma ips
(Figures 14-17). Ophiostoma polonicum and O. flex-
uosum were highly sensitive towards chitosan-
glutaraldehyde-Ag nanoparticle membrane than O.
ips and O. tetropii (Figures 14-17). Many mecha-
nisms were proposed for the antifungal property of
chitosan, among those mechanisms, the main reasons
Figure 13. Effect of silver nanoparticles on mycelial growth
of O. tetropii.
Synthesis and Characterization of Potential Fungicidal ...Velmurugan N et al.
Iranian Polymer Journal / Volume 18 Number 5 (2009) 389
www.SID.ir
8. may be due to the interaction of chitosan with cell
membrane, proteins and DNA [8]. The inhibition rate
of pure chitosan was not significant (date not shown)
compared to chitosan-silver complex. Some fungal
species are highly resistant towards pure chitosan,
since those species contain chitosan biopolymer in its
cell wall. Aspergillus sp. is the best example for chi-
tosan resistant fungus. Based on our results, wood
staining fungal species are also not sensitive towards
pure chitosan. However, chitosan was applied with
other antifungal agents, such as silver, the complex
has remarkable antifungal activity. According to liter-
ature, the chitosan with some antimicrobial agents,
such as silver, zinc, thiourea markedly inhibited the
bacterial growth relative to fungal growth [16]. The
antifungal activity of chitosan-silver ions solution
may be due to the interaction of complex with protein,
which leads to the inactivation of protein and direct
interaction with DNA, this interaction creates the
mutation and stops the replication ability of DNA.
This solution can also go through the cell wall easily,
Figure 14. Photograph of the antifungal test results of the
chitosan-glutaraldehyde membranes with different concen-
trations of silver against O. tetropii.
since those materials are very small in size. This pas-
sage can induce the cell lysis. The antifungal activity
of chitosan mainly depends on the concentration and
pH of chitosan. The antimicrobial activity of chitosan
has been studied well [4,5,8,9,12]. Literature results
showed that antifungal activity of chitosan decreased
with increasing in concentration of chitosan [12], this
is due to the formation of cross-linked structure of
chitosan amino groups through their strong intra-
molecular hydrogen bonding, which diminished the
possibilities of interaction between fungal cell mem-
brane and amino group of chitosan [12]. Microbial
growth inhibition of fungi by chitosan also depends
on the direct disturbance of cell membrane and chi-
tosan can act as a chelating agent, which can prevent
the normal nutritional pathway of fungi [12]. Hu et al.
[17] reported the antimicrobial activity of silver parti-
cles with chitosan solution. The chitosan concentra-
tion and pH of chitosan solution were chosen based
on previous reports and our laboratory results [4,16].
As shown in Figures 10-13, the antifungal activity of
Figure 15. Photograph of the antifungal test results of the
chitosan-glutaraldehyde membranes with different concen-
trations of silver against O. ips.
390 Iranian Polymer Journal / Volume 18 Number 5 (2009)
Synthesis and Characterization of Potential Fungicidal ... Velmurugan N et al.
Fungal
samples
Control
(without silver
nanoparticles)
Silver nanoparticles (percentage inhibition %)
1 ppm 10 ppm 50 ppm 100 ppm
O. ips
O. flexuosum
O. polonicum
O. tetropii
0
0
0
0
18
33
32
56
29
62
39
69
39
81
53
72
46
100
68
81
Table 2. Percentage inhibition of fungal growth in 2% MEA agar plates in the presence of silver nano-sized particles.
www.SID.ir
9. Figure 16. Photograph of the antifungal test results of the
chitosan-glutaraldehyde membranes with different concen-
trations of silver against O. polonicum.
membrane was increased with increasing concentra-
tion of Ag nano-sized particles. As mentioned above,
the antibacterial activity of chitosan has been studied
well, but the antifungal activity of chitosan varies
depending on the fungal species tested against chi-
tosan. The antifungal activity of pure chitosan was not
significant. It could be due to the repulsion or weak
interaction between the chitosan charge and negative
charge fungal cell surface. The antifungal response of
chitosan membrane and chitosan solutions is not sim-
ilar. The differences between antibacterial response of
chitosan membrane and chitosan solutions were
already reported [4]. The inhibition zone of chitosan
membrane was increased with the increasing concen-
tration of silver nano-sized particles. Thus, the results
support the role of silver nano-sized particles in anti-
fungal activity. The antifungal activity of Ag+ has
been well studied. The potential role of Ag in wood
preservation was well studied by Dorau et al. [11].
The antifungal activity of silver ions could be
described as following reasons: disruption of trans-
membrane energy metabolism and membrane elec-
tron transport chain by formation of insoluble com-
pounds in the cell wall, the formation of insoluble
compound may be due to the inactivation of cell wall
sulphhydryl group; silver ions can create mutation in
fungal DNA by displacing the hydrogen bonds; silver
ions can dissociate the enzyme complexes which are
essential for respiratory chain and membrane perme-
ability, disruption of membrane bounded enzymes
and lipids could cause the cell lysis. The chitosan pH
Figure 17. Photograph of the antifungal test results of the
chitosan-glutaraldehyde membranes with different concen-
trations of silver against O. flexuosum.
5 is also highly influenced the higher interaction
between the chitosan amine groups with electron pair
of silver nanoparticles. The different concentrations
of silver nano-sized particles play a major role in the
antifungal activity. There was no higher significant
difference identified between the antifungal activity
of silver ions at 10 ppm and 50 ppm, however, 100
ppm silver nano-sized particles containing membrane
had remarkable antifungal activity against all tested
sapstaining fungal samples. The usage of different
concentrations of silver nano-sized particles, given a
comprehensible note on the feasible amount of silver,
may be applied into chitosan membrane to produce
significant antifungal activity. Our experiment results
showed that chitosan membrane is a good carrier for
silver nano-sized particles and silver nano-sized parti-
cles can be easily incorporated with chitosan mem-
brane by treating with silver nano-sized particles solu-
tion, these silver incorporated membranes are highly
active antifungal materials. The potential of chitosan-
silver membrane and solution against wood staining
fungi has been studied well. The growth of sapstain-
ing fungi were reduced by chitosan-silver membrane
and radial growth of sapstaining fungi were also
reduced by silver nano-sized particles at minimum con-
centrations.
CONCLUSION
A hundred ppm of silver nanoparticles were com-
Synthesis and Characterization of Potential Fungicidal ...Velmurugan N et al.
Iranian Polymer Journal / Volume 18 Number 5 (2009) 391
www.SID.ir
10. pletely inhibited the growth of O. flexuosum and more
than 50% growth reduction observed in other tested
fungal samples. Same results were observed in the
chitosan membrane containing silver nano-sized par-
ticles. Thermostability of membrane was gradually
increased with addition of cross-linking agent
glutaraldehyde. In view of our experimental results,
chitosan has been identified as a good carrier for
silver ions in wood industries. The coating of chitosan
membrane incorporated with silver nano-sized
particles could reduce the fungal contamination in
wood in industries and it could be used as a
preservative material. This investigation has given the
preliminary information to determine the antifungal
activity of chitosan-silver membrane at laboratory
level. With these laboratory level results, further
investigations are currently conducted in the field to
evaluate the antifungal potential of chitosan-silver
membrane against wood staining and wood decay
fungi.
ACKNOWLEDGEMENT
This study was supported by Technology
Development Programme for Agriculture and
Forestry, Ministry of Agriculture and Forestry,
Republic of Korea (No. 105070-03-3-SB010).
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