A convenient method of synthesizing Silver Nanoparticles form Bonatea steudneri leave extract and evaluation of their electrocatalytic and phenol removal properties.

1. kfupm.edu.sa
A convenient method of synthesizing Silver
Nanoparticles form Bonatea steudneri leave
extract and evaluation of their electrocatalytic
and phenol removal properties.
Name: Shuaib Ahmed Mubarak
ID : 201708650

2. • Introduction
• Objectives
• Literature survey
• methodology
• Conclusion
• References

3. Introduction
• Nanotechnology is now considered as one of
the most important and growing fields in
research.
• metal nanoparticles have wide applications in
various fields such as medicine, electronics,
environmet, energy and catalysis .
• NPs dimensions approximately between 1-100
nanometer

4. 1μM 10μM 100μM 1mm100nm10nm1nm0.1nm
Top - Down strategy
Bottom - up strategy
Physical methods :
 photolithography
 laser-beam processing
 mechanical techniques ( grinding ,
etching , sputtering , and polishing
Wet chemcial methods:
 Organic synthesis
 Self-assembly
 Colloidal aggregation
Top-down and Bottom-up synthesis approaches

5. Metal Nano particle synthesis
Bottom - up approach Top- down approach
 Spinning
 Templates
 Plasma/flame
spraying
 Pyrolysis
 Sol jel processes
 Laser pyrolysis
 Chemical vapor
deposition
 Atomic / molecular
deposition
 Etching
 Mechanical milling
 electroexplotion
 Sputtering
 Laser ablation.
 Plants
 bacteria
 Fungi
 Algea
Green synthesis

6. Green Chemistry of the Synthesis of
Nanomaterials
• preparation of nanomaterial include chemicals
such as solvents, raw materials, reagents, and
template materials.
• Such chemicals lead to the noxious
intermediates and products.
• To diminish the undesirable products, the
concept of green chemistry was brought in the
chemical industries.

7. The advantages of green synthesis of nanoparticles over the
physical and chemical methods are:
• Clean and eco-friendly approach, as toxic
chemicals are not used;
• the active biological component itself act as
reducing and capping agent , therefore
reduction the overall cost of synthesis process
• external experimental conditions like high
energy and high pressure are not required,
leads to energy saving process.

8. • Detection of DNA
• Biolabelling
• Detection of herbicides
• Glucose sensors
Scientific
applications
• Diabitic socks
• Wound dressing
• Medical textiles
Medical
applications
• Catalyt in
• CO ox
• Benzene ox
• P-nitrophenol red
Industrial
applications
• Nanoelectrodes
• Silvernanowires
• Transistors
Electronics
Applications of silver nanoparticles

9. Reducing Agents
Ag+ ions into Ag
nanoparticles
Hydrogen
gas
Sodium
boron
hydride
Hyrazine Ethanol
Ethylene
glycols
Aliphatic
amines
Disadvantages
1.EXPENSIVE
2. HARMFUL TO THE
ENVIRONMENT

10. Plant Extract Mediated Green Synthesis of Silver Nanoparticles
• Advantages
Easily available
Safe and non-toxic
Faster than microbes
Broad variety of metabolites
Can be produces in large scale

11. Mechanism of Plant Mediated Synthesis of Silver nanoparticles
• The main mechanism considered for the route is plant-
assisted reduction due to phytochemicals.
• The main phytochemicals involved are:
– terpenoids,
– flavones,
– ketones,
– aldehydes,
– plypephenols
– amides and carboxylic acids.
Antioxidant action of
flavonoids resides mainly in
their ability to donate
electrons or hydrogen atoms
(Saxena et al. 2012)

12. (Rafique et al. 2017)
 chelate effect+
 nucleophilic
character of the
aromatic

13. (Rafique et al. 2017)

14. Objectives
• Synthesize AgNPs using Bonatea steudneri
leave extract
• Evaluate the electrocatalytic activity of the
biosynthesized AgNPs
• Evaluate phenol removal activity of the
synthesized AgNPs

15. Literature review
Table : Plants extract mediated synthesis of silver nanoparticles Plants

16. Electrocatalytic activity
(Sandeep, Santhosh, and Swamy 2016)
Cyclic voltammograms of (a) bare Gr electrode (b) and modified
Gr/AgNPs electrode in PBS, (B)

17. (Sandeep, Santhosh, and Swamy 2016)
Impedance spectrum of bare Gr/AgNPs and Gr electrodes in 5 mM Fe(CN)63-/4- solution.

18. Phenol removal
Removal of 2, 4-dichlorophenol from synthetic solution at different concentration of AgNPs.

19. Bonatea steudneri
• commonly known as the Arabian
Bonatea
• is a perennial herb which can grow
to 25 to 125 centimeters in height
• Arabian Bonatea is native to Saudi
Arabia, Yemen, and the East and
North Africa
• has a significant horticultural value
in Saudi Arabia
• is the family of Orchids, which is
reported to contain phytochemicals
such as alkaloids, triterpenoids,
flavonoids and stilbenoids

20. Synthesis of Silver nanoparticles
 Boiled 25 g of Bonatea steudneri leaves in
100 mL distilled water will be boiled for 5 min
& filter
 15 mL of this extract will be added to 100
mL of 1 mM silver nitrate and heated to 75ºC
 Maintain this temp. for the entire reaction
(different plant extracts require different times
to complete the reaction)
 The product will then be Centrifuged and
washed with distilled water.

21.  UV-Vis Spectroscopy
 Transmission Electron Microscopy (TEM)
 Energy Dispersive X- Ray Spectroscopy (EDX)
 thermo gravimetric analysis (TGA)
 X-Ray Diffraction (XRD)
 Fourier Transform Infra Red Spectroscopy
(FTIR)
Characterization of Silver nanoparticles

22. Electro catalytic activity of the biosynthesized AgNPs
The Graphite electrode ( Gr) surface polished with emery papers.
The Gr electrode is then sonicated, rinsed in milli-Q water and dried.
A suspension of AgNPs in deionized water (w/v ratio of 10 mg in 1 mL) is prepared
and drop casted onto the Gr surface.
The resulting modified Gr electrode (Gr/AgNPs) is dried and stored until further
usage.
The electrocatalytic behavior of bare Gr and modified Gr/AgNPs is investigated by
cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)
All electrochemical experiments are carried out in a three electrode cell with
Gr/AgNPs as working electrode, standard calomel electrode (SCE) as reference
electrode and platinum wire as auxiliary electrode

23. Phenol removal
 solution of 2, 4-dichlorophenol (2, 4 DCP) will be used for the analysis of phenol
remediation.
 25 mL of 2, 4-DCP stock solution will be taken in six different 50 mL Erlenmeyer’
flasks.
 different quantities of biosynthesized AgNPs (10, 20, 30, 40 and 50 mg) will be
added to these flasks.
 The AgNPs will be dispersed and stirred for 30 min . The reaction will be left for
12H at room temperature
 The reaction mixture will be analyzed for phenolic content by 4-amino antipyrene
method.
 25 mL of sample will be treated with 0.625 mL of NH4OH (0.5N) solution and the
should be adjusted to 7.9?0.1 with phosphate buffer solution (PBS).
 0.25 mL of 4-amino antipyrine (4 %) and 0.25 mL of potassium ferricyanide (8 %) will
be added to above solution,
 mixed well and left for 15 minutes.
 The absorbance is measured at 500 nm after 15 minutes and the residual 2, 4-DCP
concentration will be determined from standard curve.

24. Conclusion
A novel plant leaf extract Bonatea steudneri is expected to
reduce silver nitrate to silver Nano particles with desirable
particle size. The synthesized AgNPs is expected to have
potential electrochemical activity and also remove phenolic
compounds from aqueous solutions.
Based on the results found , the AgNPs can be used in
electrochemical sensing and phenol remediation.

25. References
• T. M. Abdelghany et al., “Recent Advances in Green Synthesis of Silver
Nanoparticles and Their Applications: About Future Directions. A Review,”
Bionanoscience, vol. 8, no. 1, pp. 5–16, Mar. 2018.
• S. Collenette, “Orchids in Saudi Arabia,” Am. Orchid Soc. Bull., 1990.
• T. M. Abdelghany et al., “Recent Advances in Green Synthesis of Silver
Nanoparticles and Their Applications: About Future Directions. A Review,”
Bionanoscience, vol. 8, no. 1, pp. 5–16, Mar. 2018.
• P. Logeswari, S. Silambarasan, and J. Abraham, “Synthesis of silver
nanoparticles using plants extract and analysis of their antimicrobial
property,” J. Saudi Chem. Soc., vol. 19, no. 3, pp. 311–317, 2015.
• Rauwel, S. Küünal, S. Ferdov, and E. Rauwel, “A review on the green
synthesis of silver nanoparticles and their morphologies studied via TEM,”
Adv. Mater. Sci. Eng., vol. 2015, 2015.