Alternate animal experiments models for pre and post clinical screening of new drugs.
#Expetrimental_Pharmacology.
#Preclinical Screening methods and testing models.
#Animal_Handeling
The document discusses alternatives to animal testing that can provide more accurate and humane methods for toxicity testing and medical research. Some key alternatives mentioned include cell cultures grown from human tissues, microdosing studies conducted on human volunteers, computer simulations, and organ-on-chip models containing living human cells. While alternatives reduce animal use, the document notes they cannot fully replace whole-body testing in animals, which is still needed to determine drug safety and efficacy for approval. The goal is to continue advancing alternative methods to refine, reduce and replace animal experiments wherever possible.
This document discusses alternatives to animal testing for drug development and safety testing. It outlines 4 steps to developing and validating alternative methods: defining the alternative, developing the alternative using in vitro and computer models, validating the methods, and gaining acceptance. Recent advances in stem cells and organ-on-chip models provide more human-relevant alternatives. In vitro tests using human cells, tissues, and organ models can replace animal use for toxicity, efficacy, and disease modeling. Computer models also simulate human biology for drug screening without animals.
This document discusses several alternative methods that can be used instead of animal experiments for pharmacological and toxicological screening. It describes the full thickness skin model method which uses skin tissue to evaluate the effects of substances instead of live animals. It also mentions in silico methods which use computer programs and knowledge of similar substances to predict properties without testing. The document outlines the cell line technique using continuous cell lines to screen for effects like anticancer drugs. Finally, it explains the patch clamp technique which studies individual ion channels in isolated cells and kidney tubules as an alternative to testing on whole animals.
Alternative to Animal Experiment ModelsDr Jayant Rai
The document discusses alternatives to animal experimentation. It provides an overview of animal experimentation, including its historical use and current regulatory guidelines. Some key uses of animals in experimentation include education, research, cosmetic testing, and toxicology testing. The document then discusses the development of alternatives such as in vitro techniques like cell cultures, microorganism studies, computer simulations, and epidemiological research that can replace or reduce animal use. It provides examples of specific alternative tests and methods that have been validated including embryonic stem cell tests, the Ames test, and skin patch tests. Overall, the document promotes developing and validating alternative methods to animal testing that satisfy the principles of replacement, reduction and refinement of animal use.
The document discusses alternatives to animal experiments that can be used in biomedical research and testing. It covers 3R strategies like refinement, reduction and replacement of animal experiments. Some alternatives mentioned include in vitro cell and tissue culture methods, computer-based models, microdosing studies and quantitative structure-activity relationships. The summary provides an overview of the different alternative methods discussed in the document like in vitro toxicity testing, in chemico tests, computer-assisted learning programs, microfluidic chips and in silico models. The use of these alternatives can help reduce animal experiments while making toxicity testing more accurate and reliable.
This document summarizes screening methods for evaluating potential anti-inflammatory drugs. It discusses the inflammatory response and various animal models used to test drug candidates, including carrageenan-induced paw edema, cotton pellet-induced granuloma, and UVB-induced erythema in guinea pigs. Several in vitro assays are also described, such as measuring COX inhibition and evaluating the ability of drugs to block mast cell degranulation and platelet-neutrophil adhesion. The goal of these screening methods is to effectively identify drug candidates that can target different phases and components of the inflammatory process.
Cancer is characterized by uncontrolled cell proliferation. Many factors can cause cancer, including external factors like chemicals and radiation, and internal factors like hormones and genetic mutations. While there are 92 approved anticancer drugs, effective therapies are still lacking for many types of cancer. New drugs are needed that are more selective for cancer cells to reduce side effects from long-term treatment. In vitro screening methods are used to identify potential drug candidates, including assays to test cell viability, proliferation, and morphology. Promising candidates then advance to in vivo testing using animal models of cancer like chemically-induced tumors in mice. The goal is to find drugs that can effectively treat cancer while avoiding side effects.
The document discusses alternatives to animal testing that can provide more accurate and humane methods for toxicity testing and medical research. Some key alternatives mentioned include cell cultures grown from human tissues, microdosing studies conducted on human volunteers, computer simulations, and organ-on-chip models containing living human cells. While alternatives reduce animal use, the document notes they cannot fully replace whole-body testing in animals, which is still needed to determine drug safety and efficacy for approval. The goal is to continue advancing alternative methods to refine, reduce and replace animal experiments wherever possible.
This document discusses alternatives to animal testing for drug development and safety testing. It outlines 4 steps to developing and validating alternative methods: defining the alternative, developing the alternative using in vitro and computer models, validating the methods, and gaining acceptance. Recent advances in stem cells and organ-on-chip models provide more human-relevant alternatives. In vitro tests using human cells, tissues, and organ models can replace animal use for toxicity, efficacy, and disease modeling. Computer models also simulate human biology for drug screening without animals.
This document discusses several alternative methods that can be used instead of animal experiments for pharmacological and toxicological screening. It describes the full thickness skin model method which uses skin tissue to evaluate the effects of substances instead of live animals. It also mentions in silico methods which use computer programs and knowledge of similar substances to predict properties without testing. The document outlines the cell line technique using continuous cell lines to screen for effects like anticancer drugs. Finally, it explains the patch clamp technique which studies individual ion channels in isolated cells and kidney tubules as an alternative to testing on whole animals.
Alternative to Animal Experiment ModelsDr Jayant Rai
The document discusses alternatives to animal experimentation. It provides an overview of animal experimentation, including its historical use and current regulatory guidelines. Some key uses of animals in experimentation include education, research, cosmetic testing, and toxicology testing. The document then discusses the development of alternatives such as in vitro techniques like cell cultures, microorganism studies, computer simulations, and epidemiological research that can replace or reduce animal use. It provides examples of specific alternative tests and methods that have been validated including embryonic stem cell tests, the Ames test, and skin patch tests. Overall, the document promotes developing and validating alternative methods to animal testing that satisfy the principles of replacement, reduction and refinement of animal use.
The document discusses alternatives to animal experiments that can be used in biomedical research and testing. It covers 3R strategies like refinement, reduction and replacement of animal experiments. Some alternatives mentioned include in vitro cell and tissue culture methods, computer-based models, microdosing studies and quantitative structure-activity relationships. The summary provides an overview of the different alternative methods discussed in the document like in vitro toxicity testing, in chemico tests, computer-assisted learning programs, microfluidic chips and in silico models. The use of these alternatives can help reduce animal experiments while making toxicity testing more accurate and reliable.
This document summarizes screening methods for evaluating potential anti-inflammatory drugs. It discusses the inflammatory response and various animal models used to test drug candidates, including carrageenan-induced paw edema, cotton pellet-induced granuloma, and UVB-induced erythema in guinea pigs. Several in vitro assays are also described, such as measuring COX inhibition and evaluating the ability of drugs to block mast cell degranulation and platelet-neutrophil adhesion. The goal of these screening methods is to effectively identify drug candidates that can target different phases and components of the inflammatory process.
Cancer is characterized by uncontrolled cell proliferation. Many factors can cause cancer, including external factors like chemicals and radiation, and internal factors like hormones and genetic mutations. While there are 92 approved anticancer drugs, effective therapies are still lacking for many types of cancer. New drugs are needed that are more selective for cancer cells to reduce side effects from long-term treatment. In vitro screening methods are used to identify potential drug candidates, including assays to test cell viability, proliferation, and morphology. Promising candidates then advance to in vivo testing using animal models of cancer like chemically-induced tumors in mice. The goal is to find drugs that can effectively treat cancer while avoiding side effects.
Anesthesia and euthanasia of experimental animal by vivek and naveenAnimatedWorld
Anesthesia and euthanasia of experimental animal by vivek and naveen
Anesthesia
It is a state of controlled temporary loss of sensation or awareness that or awareness that is induced for medical purpose.
Anesthetic agents
The anesthetic agents are great and choosing the correct one for particular suggestion.
In laboratory animal field , the anesthetic surgeon and post operative are often one and the same person.
This will help to chose correct drug for anaesthesia.
Sometime the wise anesthetic agents also cause undesirable responses. so, its responsibility of experimenters to document this advance in exprimental protocol
Euthanasia
The term euthanasia is derived from the Greek terms eu mean good and thanatos mean death.
Euthanasia is the act of including humane death in an animal. sacrificing the experimental animal after use by gentle procedure causing minimum of physical and mental suffering is called euthanasia.
Alternative methods to animals testing are the development and implementation of test method that avoid use of live animals or use of less animals in method.
The council directive on protection of animals used for experiments and scientific purpose in article 23
“The commission and member states should encourage
research into development and validation of alternative methods which could provide the same level of information as that obtained in experiment using animals but which involves less animal”.
Alternative methods able to do:
Reduce Refine Replace
collectively called as “The 3Rs Principle”.
Needs for alternative methods
Because in laboratory animals may be:
Poisoned.
Deprived of food water and sleep.
Applied with skin and eye irritants.
Subjected to psychological stress.
Deliberately infected with the infected disease.
The document summarizes screening models for Alzheimer's disease. It describes two in vitro methods - inhibition of acetylcholine-esterase activity in rat striatum and inhibition of butyrylcholine-esterase activity in human serum. It also describes two in vivo methods - the step-down test and scopolamine-induced amnesia in mice. The step-down test measures learning and memory in rats using an elevated platform and electric shock. The scopolamine-induced amnesia test measures the ability of drugs to reverse memory deficits caused by the antimuscarinic scopolamine in mice. The document provides details on the procedures and evaluations of these screening methods.
1. The document discusses alternatives to animal experiments, including in vitro methods like cell and tissue culture, computer modeling, and microdosing.
2. Specific alternatives mentioned include the embryonic stem cell test, Limulus amoebocyte lysate test, organ-on-chip models, and the local lymph node assay.
3. The principles of replacement, reduction and refinement of animal experiments are also covered, along with relevant laws and the need to minimize harm to animals in research.
Screening models for aphrodisiac agents and anti fertility agentsCh. Bhargava krishna
This document discusses guidelines for conducting studies on aphrodisiac and anti-fertility agents in animals. It outlines various in vivo and in vitro screening models used to test the effects of these drugs, including mating behavior tests, libido tests, assessment of sperm parameters, and estimation of sex hormone levels. The guidelines specify how to properly house and train male and female animals, and evaluate behaviors like mounting frequency, intromission latency, and ejaculation. Common aphrodisiac drugs discussed include sildenafil, arginine, and testosterone, while benefits and screening of anti-fertility agents are also summarized.
The guidelines describe about the subacute toxicity studies in rodents with a comparison with the previous guideline.it also includes the comparison of all three subacute toxicity studies OECD 407, OECD 410, and OECD 412
Introduction to Screening Models Of Anti Cancer Drugs
Need for novel anti cancer drugs, In - vitro methods, In - vivo methods, Advantages and disadvantages
Presented by
T. Niranjan Reddy
Department of Pharmacology
This seminar is my attempt to discuss screening of anti-emetic drugs using different animal models. The materials used in the presentation is derived from different standard textbooks, internet and journals. Please feel free to suggest ways to improve it.
screening methods for anti-atherosclerotic agentsPrajitha p
This document summarizes various screening methods for evaluating potential anti-atherosclerotic agents. It discusses methods such as inducing atherosclerosis in animal models like rabbits fed a high-cholesterol diet and evaluating the effects of test compounds. It also covers assays measuring effects on lipid metabolism like inhibiting cholesterol biosynthesis and absorption. Specific assays are described in detail, including the procedures, evaluations, and purposes of evaluating agents' abilities to inhibit enzymes involved in cholesterol synthesis.
Assignment on Alternatives to Animal Screening MethodDeepak Kumar
Toxicity studies are generally performed to determine drug effects that cannot be evaluated through standard pharmacology profiles or that only occur with repeated administration. Most toxicity tests are performed in two species, such as a rodent and non-rodent, to avoid overlooking unexpected adverse effects before introducing new chemical entities into humans. While animal testing has faced controversy, alternatives using biotechnology tools such as transgenic animal models, cell cultures, and in silico methods are being developed wherever possible to reduce animal use. These alternative techniques include cell line techniques, full thickness skin models, and patch clamp methods to study ion channels at a cellular level.
This document summarizes models used to study anti-emetic drugs. It describes various in vivo, in vitro, and human models. For in vivo models, it outlines drug-induced (cisplatin, apomorphine, copper sulfate), motion, and radiation models using species like dogs, cats, ferrets, and rats. It discusses parameters assessed like retching episodes. For in vitro models, it notes evaluating drugs' activity at 5-HT3 receptors. Finally, it mentions human models like using apomorphine or ipecac to induce vomiting and assessing drug effectiveness.
Alternative methods to animal testing: reviewankit sharma
Animals are used in various areas of biomedical science such as teaching, research, and testing of drugs. While animal models provide important insights, they have limitations in translating findings to humans due to interspecies differences. To reduce animal use, alternatives such as computer modeling, tissue cultures, and microdosing are being utilized. The 3Rs principle of replacement, reduction, and refinement is also applied to minimize animal pain and distress when animal use is necessary.
Preclinical screening methods of cns stimulantsRashmi116
This document describes various preclinical screening methods used to evaluate central nervous system (CNS) stimulants. It discusses behavioral manifestations of CNS stimulation like increased alertness. Various screening methods are described including the actophotometer test to measure locomotor activity, strychnine-induced convulsion test, sand displacement test, runway test and others. Each test is briefly explained along with its purpose, procedure, and evaluation method. A variety of behavioral tests in animals are used to screen for CNS stimulant activity of novel compounds.
This document summarizes various screening models for diabetes, including in vivo and in vitro models. For in vivo models, it describes chemically-induced diabetes models using alloxan and streptozotocin in rodents, as well as spontaneous genetic rodent models like BB rats and KK mice. In vitro models discussed include isolated pancreatic islet cells, cultured human myotubes for glucose uptake studies, and the Gluc-HET chick embryo model for assessing insulin mimetic compounds. The document provides details on the procedures, advantages and limitations of each type of screening model.
This document provides information on screening methods for antidiabetic drugs. It discusses various in vivo and in vitro models used to screen drugs, including chemically-induced diabetes models using alloxan and streptozotocin in animals, genetically diabetic animal models like NOD mice and BB rats, and isolated tissue and cell-based in vitro models. A variety of animal models aim to mimic types 1 and 2 diabetes by destroying pancreatic beta cells or inducing insulin resistance. These preclinical models are used to evaluate new drugs' ability to lower blood glucose levels and treat diabetes symptoms before human trials.
Alternative methods to animal toxicity testingSachin Sharma
This document presents information on alternative methods to animal toxicity testing. It discusses the need for alternatives due to the harms animals face in toxicity testing. The 3Rs principles of reduction, refinement and replacement are explained, which aim to minimize animal use and suffering. The validation process for new alternative methods through organizations like ECVAM is covered. Specific alternative methods mentioned include in vitro tests like the Ames test and HET-CAM test, in silico methods, and mathematical models. The HET-CAM test for eye irritation is described in more detail.
Assignment on Limitation of animal experimentationDeepak Kumar
This document discusses 5 key causes for why animal studies do not reliably predict human outcomes: 1) Interspecies differences in disease susceptibility, drug metabolism, and other factors; 2) Stressful laboratory environments and common procedures that alter animal physiology; 3) False positives from chronic high-dose rodent studies that overwhelm natural defenses; 4) Poor methodological quality in many animal experiments lacking randomization, blinding, and other controls; 5) Publication and other biases that skew available data.
This document summarizes various preclinical screening methods used to evaluate potential anti-epileptic drugs. It describes several animal models of induced seizures including electroshock seizures, chemical-induced seizures using pentylenetetrazol or picrotoxin. It also discusses genetic models like the totterer mouse that is prone to spontaneous seizures. The key methods are maximal electroshock in mice/rats to test generalized tonic-clonic seizure protection and the pentylenetetrazol test in mice to assess anticonvulsant effects against petit mal-like seizures. These preclinical tests aim to predict potential efficacy of new compounds before clinical trials in humans.
This document discusses screening methods for anticancer agents. It describes both in vitro and in vivo methods. For in vitro screening, assays are discussed that measure cell viability, proliferation, and cytotoxicity, such as the MTT assay, Sulforhodamine B assay, 3H-thymidine uptake assay, and dye exclusion tests. For in vivo screening, models are described that use chemically-induced tumors in animals as well as cell line and xenograft transplant models to test potential anticancer agents and measure effects on tumor growth.
The document discusses alternatives to animal studies in 3 main areas: replacement, reduction, and refinement. Replacement involves using non-animal methods like cell cultures, computer simulations, or chemical/physical systems. Reduction aims to minimize animal use through improved study design and statistics. Refinement focuses on lessening pain and distress for animals, such as by improving surgical techniques or control of variables. A variety of non-animal methods are presented, including in vitro assays, microorganisms, and computer models, as well as strategies for reducing animal numbers through data sharing and statistical practices.
The document discusses animal testing and some of the ethical issues surrounding it. It notes that while controversial, animal research has allowed medical and scientific advances. Around 50-100 million animals are used in research each year. The document explores how pain and suffering in animal testing is measured, and outlines some of the arguments made by proponents and opponents of animal testing. It also discusses alternatives to animal testing such as the three R's of replacement, reduction and refinement.
Anesthesia and euthanasia of experimental animal by vivek and naveenAnimatedWorld
Anesthesia and euthanasia of experimental animal by vivek and naveen
Anesthesia
It is a state of controlled temporary loss of sensation or awareness that or awareness that is induced for medical purpose.
Anesthetic agents
The anesthetic agents are great and choosing the correct one for particular suggestion.
In laboratory animal field , the anesthetic surgeon and post operative are often one and the same person.
This will help to chose correct drug for anaesthesia.
Sometime the wise anesthetic agents also cause undesirable responses. so, its responsibility of experimenters to document this advance in exprimental protocol
Euthanasia
The term euthanasia is derived from the Greek terms eu mean good and thanatos mean death.
Euthanasia is the act of including humane death in an animal. sacrificing the experimental animal after use by gentle procedure causing minimum of physical and mental suffering is called euthanasia.
Alternative methods to animals testing are the development and implementation of test method that avoid use of live animals or use of less animals in method.
The council directive on protection of animals used for experiments and scientific purpose in article 23
“The commission and member states should encourage
research into development and validation of alternative methods which could provide the same level of information as that obtained in experiment using animals but which involves less animal”.
Alternative methods able to do:
Reduce Refine Replace
collectively called as “The 3Rs Principle”.
Needs for alternative methods
Because in laboratory animals may be:
Poisoned.
Deprived of food water and sleep.
Applied with skin and eye irritants.
Subjected to psychological stress.
Deliberately infected with the infected disease.
The document summarizes screening models for Alzheimer's disease. It describes two in vitro methods - inhibition of acetylcholine-esterase activity in rat striatum and inhibition of butyrylcholine-esterase activity in human serum. It also describes two in vivo methods - the step-down test and scopolamine-induced amnesia in mice. The step-down test measures learning and memory in rats using an elevated platform and electric shock. The scopolamine-induced amnesia test measures the ability of drugs to reverse memory deficits caused by the antimuscarinic scopolamine in mice. The document provides details on the procedures and evaluations of these screening methods.
1. The document discusses alternatives to animal experiments, including in vitro methods like cell and tissue culture, computer modeling, and microdosing.
2. Specific alternatives mentioned include the embryonic stem cell test, Limulus amoebocyte lysate test, organ-on-chip models, and the local lymph node assay.
3. The principles of replacement, reduction and refinement of animal experiments are also covered, along with relevant laws and the need to minimize harm to animals in research.
Screening models for aphrodisiac agents and anti fertility agentsCh. Bhargava krishna
This document discusses guidelines for conducting studies on aphrodisiac and anti-fertility agents in animals. It outlines various in vivo and in vitro screening models used to test the effects of these drugs, including mating behavior tests, libido tests, assessment of sperm parameters, and estimation of sex hormone levels. The guidelines specify how to properly house and train male and female animals, and evaluate behaviors like mounting frequency, intromission latency, and ejaculation. Common aphrodisiac drugs discussed include sildenafil, arginine, and testosterone, while benefits and screening of anti-fertility agents are also summarized.
The guidelines describe about the subacute toxicity studies in rodents with a comparison with the previous guideline.it also includes the comparison of all three subacute toxicity studies OECD 407, OECD 410, and OECD 412
Introduction to Screening Models Of Anti Cancer Drugs
Need for novel anti cancer drugs, In - vitro methods, In - vivo methods, Advantages and disadvantages
Presented by
T. Niranjan Reddy
Department of Pharmacology
This seminar is my attempt to discuss screening of anti-emetic drugs using different animal models. The materials used in the presentation is derived from different standard textbooks, internet and journals. Please feel free to suggest ways to improve it.
screening methods for anti-atherosclerotic agentsPrajitha p
This document summarizes various screening methods for evaluating potential anti-atherosclerotic agents. It discusses methods such as inducing atherosclerosis in animal models like rabbits fed a high-cholesterol diet and evaluating the effects of test compounds. It also covers assays measuring effects on lipid metabolism like inhibiting cholesterol biosynthesis and absorption. Specific assays are described in detail, including the procedures, evaluations, and purposes of evaluating agents' abilities to inhibit enzymes involved in cholesterol synthesis.
Assignment on Alternatives to Animal Screening MethodDeepak Kumar
Toxicity studies are generally performed to determine drug effects that cannot be evaluated through standard pharmacology profiles or that only occur with repeated administration. Most toxicity tests are performed in two species, such as a rodent and non-rodent, to avoid overlooking unexpected adverse effects before introducing new chemical entities into humans. While animal testing has faced controversy, alternatives using biotechnology tools such as transgenic animal models, cell cultures, and in silico methods are being developed wherever possible to reduce animal use. These alternative techniques include cell line techniques, full thickness skin models, and patch clamp methods to study ion channels at a cellular level.
This document summarizes models used to study anti-emetic drugs. It describes various in vivo, in vitro, and human models. For in vivo models, it outlines drug-induced (cisplatin, apomorphine, copper sulfate), motion, and radiation models using species like dogs, cats, ferrets, and rats. It discusses parameters assessed like retching episodes. For in vitro models, it notes evaluating drugs' activity at 5-HT3 receptors. Finally, it mentions human models like using apomorphine or ipecac to induce vomiting and assessing drug effectiveness.
Alternative methods to animal testing: reviewankit sharma
Animals are used in various areas of biomedical science such as teaching, research, and testing of drugs. While animal models provide important insights, they have limitations in translating findings to humans due to interspecies differences. To reduce animal use, alternatives such as computer modeling, tissue cultures, and microdosing are being utilized. The 3Rs principle of replacement, reduction, and refinement is also applied to minimize animal pain and distress when animal use is necessary.
Preclinical screening methods of cns stimulantsRashmi116
This document describes various preclinical screening methods used to evaluate central nervous system (CNS) stimulants. It discusses behavioral manifestations of CNS stimulation like increased alertness. Various screening methods are described including the actophotometer test to measure locomotor activity, strychnine-induced convulsion test, sand displacement test, runway test and others. Each test is briefly explained along with its purpose, procedure, and evaluation method. A variety of behavioral tests in animals are used to screen for CNS stimulant activity of novel compounds.
This document summarizes various screening models for diabetes, including in vivo and in vitro models. For in vivo models, it describes chemically-induced diabetes models using alloxan and streptozotocin in rodents, as well as spontaneous genetic rodent models like BB rats and KK mice. In vitro models discussed include isolated pancreatic islet cells, cultured human myotubes for glucose uptake studies, and the Gluc-HET chick embryo model for assessing insulin mimetic compounds. The document provides details on the procedures, advantages and limitations of each type of screening model.
This document provides information on screening methods for antidiabetic drugs. It discusses various in vivo and in vitro models used to screen drugs, including chemically-induced diabetes models using alloxan and streptozotocin in animals, genetically diabetic animal models like NOD mice and BB rats, and isolated tissue and cell-based in vitro models. A variety of animal models aim to mimic types 1 and 2 diabetes by destroying pancreatic beta cells or inducing insulin resistance. These preclinical models are used to evaluate new drugs' ability to lower blood glucose levels and treat diabetes symptoms before human trials.
Alternative methods to animal toxicity testingSachin Sharma
This document presents information on alternative methods to animal toxicity testing. It discusses the need for alternatives due to the harms animals face in toxicity testing. The 3Rs principles of reduction, refinement and replacement are explained, which aim to minimize animal use and suffering. The validation process for new alternative methods through organizations like ECVAM is covered. Specific alternative methods mentioned include in vitro tests like the Ames test and HET-CAM test, in silico methods, and mathematical models. The HET-CAM test for eye irritation is described in more detail.
Assignment on Limitation of animal experimentationDeepak Kumar
This document discusses 5 key causes for why animal studies do not reliably predict human outcomes: 1) Interspecies differences in disease susceptibility, drug metabolism, and other factors; 2) Stressful laboratory environments and common procedures that alter animal physiology; 3) False positives from chronic high-dose rodent studies that overwhelm natural defenses; 4) Poor methodological quality in many animal experiments lacking randomization, blinding, and other controls; 5) Publication and other biases that skew available data.
This document summarizes various preclinical screening methods used to evaluate potential anti-epileptic drugs. It describes several animal models of induced seizures including electroshock seizures, chemical-induced seizures using pentylenetetrazol or picrotoxin. It also discusses genetic models like the totterer mouse that is prone to spontaneous seizures. The key methods are maximal electroshock in mice/rats to test generalized tonic-clonic seizure protection and the pentylenetetrazol test in mice to assess anticonvulsant effects against petit mal-like seizures. These preclinical tests aim to predict potential efficacy of new compounds before clinical trials in humans.
This document discusses screening methods for anticancer agents. It describes both in vitro and in vivo methods. For in vitro screening, assays are discussed that measure cell viability, proliferation, and cytotoxicity, such as the MTT assay, Sulforhodamine B assay, 3H-thymidine uptake assay, and dye exclusion tests. For in vivo screening, models are described that use chemically-induced tumors in animals as well as cell line and xenograft transplant models to test potential anticancer agents and measure effects on tumor growth.
The document discusses alternatives to animal studies in 3 main areas: replacement, reduction, and refinement. Replacement involves using non-animal methods like cell cultures, computer simulations, or chemical/physical systems. Reduction aims to minimize animal use through improved study design and statistics. Refinement focuses on lessening pain and distress for animals, such as by improving surgical techniques or control of variables. A variety of non-animal methods are presented, including in vitro assays, microorganisms, and computer models, as well as strategies for reducing animal numbers through data sharing and statistical practices.
The document discusses animal testing and some of the ethical issues surrounding it. It notes that while controversial, animal research has allowed medical and scientific advances. Around 50-100 million animals are used in research each year. The document explores how pain and suffering in animal testing is measured, and outlines some of the arguments made by proponents and opponents of animal testing. It also discusses alternatives to animal testing such as the three R's of replacement, reduction and refinement.
This document discusses the history and types of animal experimentation. It notes that Aristotle and Erasistratus were among the first to use living animals in experiments. It outlines the types of animal research including basic research, applied research, toxicology testing, and xenotransplantation. Common animal models used are rats, mice, rabbits, and guinea pigs. The document also discusses the principles of replacement, reduction and refinement of animal experiments and the ethical requirements for conducting such research.
Chapter 2- research involving animals .pptxHendmaarof
Researchers use animal models in research to understand human physiology, diseases, and develop new treatments. Animals are used because they share many biological similarities to humans despite differences in appearance. The 3Rs principles of replacement, reduction, and refinement guide researchers to replace animal use when possible, reduce the number of animals used, and refine experiments to minimize animal suffering. While some disagree with animal research, proponents argue it has advanced medical knowledge and led to treatments for conditions like polio, cystic fibrosis, and stroke.
Animal Experimentation- Contemporary IssueChandan Saha
Animals have their own rights. They are not puppet of our laboratory. With the help of modern and scientific technology we can change old traditional animal experiment methods.
Animal testing is commonly used in scientific research and development. It helps ensure product safety and advance medical research. However, it raises ethical issues and alternatives are being developed. Reduction methods aim to minimize the number of animals used through better study design and data sharing. Refinement improves animal welfare. Replacement seeks to avoid animal use by developing alternative methods like computer modeling, microfluidics, and serum-free cell cultures. As technology advances, more research can be done without harming animals.
Today there exists a wide spectrum of views on this subject, ranging from those concerned with animal 'rights' to those who view animals only as a resource to be exploited.
All of thThe five freedoms were originally developed from a UK Government report on livestock husbandry in 1965 (Prof.Roger Brambell) then by Farm Animal Welfare Council (FAWC) In July 1979
Freedom from hunger or thirst by ready access to fresh water and a diet to maintain full health and vigour .
Freedom from discomfort by providing an appropriate environment including shelter and a comfortable resting area .
Freedom from pain, injury or disease by prevention or rapid diagnosis and treatment.
Freedom to express (most) normal behaviour by providing sufficient space, proper facilities and company of the animal's own kind.
Freedom from fear and distress by ensuring conditions and treatment which avoid mental suffering.
This document discusses the use of animal models in biomedical research. It notes that animal models allow researchers to study disease states and potential treatments in ways that would not be possible or ethical in human subjects. Common animal models used include mice, rats, dogs, primates, and others depending on the research goal. The document outlines some examples of procedures performed on animal models, including inducing conditions like heart attacks, migraines, and spinal cord injuries. It also discusses the large number of animals and costs involved in developing and testing biological products like vaccines.
This document discusses the use of animal models in biomedical research. It describes how animals are used to study human disease and test potential treatments in order to advance human health without risking harm to people. Common animal models mentioned include mice, rats, dogs, primates, and rabbits, which can provide insights into conditions like heart disease, cancer, neurological disorders, and infectious diseases. However, the use of animals in research also raises ethical issues, as it often involves invasive procedures that can cause pain and distress. Large numbers of animals are required for activities like vaccine production and drug testing, but only a small percentage of potential treatments ultimately succeed.
The document discusses alternatives to animal testing in research. It notes that while animals have traditionally been used for testing, this method is cruel, time-consuming, and not predictive of human outcomes. New alternative methods include in vitro, in silico, in chemico techniques using cells, tissues, organs-on-chips and computer modeling that can replace many animal tests in a more humane, effective and predictive way. Widespread adoption of alternative methods can improve both the ethics and accuracy of medical research.
Medical research using animals has a long history dating back to Louis Pasteur and Ivan Pavlov in the late 19th century. Common animals used in medical research include mice, rats, dogs, cats, and primates due to their genetic and physiological similarities to humans. While controversial, animal research has led to major medical advances such as the polio vaccine and insulin. Guidelines aim to reduce animal usage and minimize suffering through the Three Rs principles.
Alternatives to animal studies in Pharmaceutical research has been explained on the basis of replacement, reduction and refinement. Also newer pre-clinical animal models like use of genetically modified animals were presented.
Experimental pharmacology involves studying the effects of pharmacological agents on animals to determine pharmacokinetic and pharmacodynamic parameters. It is done to aid in curing human diseases but is debated due to harm caused to animals. Some organizations suggest abolishing animal testing and using alternative methods instead, though researchers argue alternatives may not yield desired results or produce errors. The current scenario involves minimizing animal use after careful verification while alternatives save animals but may be less accurate and time-consuming than animal testing.
Pharmacology (introduction and handling of experimental animals)Osama Al-Zahrani
1) The document discusses pharmacology practical experiments involving handling experimental animals.
2) It introduces different levels of pharmacological testing from pre-clinical to clinical trials in humans.
3) The main focus is on how to properly handle experimental animals like rats, mice, and rabbits used in studies, including how to house them, provide water, and restrain them safely during experiments.
This document discusses alternatives to animal testing for biomedical research. It begins by outlining some common uses of animal testing and the need for alternative experiments due to disadvantages like pain caused to animals. It then describes some laws and regulations regarding animal testing. The 5 R's framework of replacement, reduction and refinement of animal experiments is explained. Various alternative methods like in vitro cell and tissue cultures, in chemico tests, and in silico computer modeling are presented. Specific examples of alternative tests like embryonic stem cell assays and pyrogen tests are also provided.
This document provides an overview of experimental toxicology and the use of animal models. It discusses the importance of animal models in toxicity testing and research. It covers key aspects of conducting animal studies including the four R's (replacement, reduction, refinement, and responsibility), selecting appropriate animal models and strains, husbandry and care of animals, dosing techniques, and understanding animal physiology. The origins of predictive animal testing are reviewed through important cases that prompted regulations requiring pre-market safety testing of drugs and chemicals.
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Slide contains aspects of animal use in pharmacology laboratory.
Along with CPCSEA Guidelines (now CCSEA).
Laboratory animals experiment benefits as well as limitations.
Different animals used in laboratory.
Ethical issues related to animal biotechnologyKAUSHAL SAHU
Introduction
Why are genetically modified animals produced?
Examples of transgenic animals
Why are animals used instead of genetically modified microbes or plants?
Ethical issues
Religious concerns
Responsibility of Scientists
Need for Guidelines
Conclusion
References
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1. ALTERNATE ANIMALALTERNATE ANIMAL
EXPERIMENTSEXPERIMENTS
Presented by :-Presented by :-
Deepak Chandra JoshiDeepak Chandra Joshi
M.Pharma (Pharmacology)M.Pharma (Pharmacology)
Department of Pharmaceutical SciencesDepartment of Pharmaceutical Sciences
Bhimtal (Nainital)Bhimtal (Nainital)
2. Table of Content :-Table of Content :-
IntroductionIntroduction
Need for alternative to animalNeed for alternative to animal
Laws and regulationsLaws and regulations
Alternative experiments methodsAlternative experiments methods
1.1. RefinementRefinement
2.2. ReductionReduction
3.3. ReplacementReplacement
In-Vitro MethodsIn-Vitro Methods
3. IntroductionIntroduction
Alternative animal experiment are the develepmAlternative animal experiment are the develepm
-ent and Implementation of test method’s that-ent and Implementation of test method’s that
avoid the use of life animals.avoid the use of life animals.
The primary goal of alternative animal experimeThe primary goal of alternative animal experime
-nt is the minimize pain which is suffer by-nt is the minimize pain which is suffer by
animal during the experiments.animal during the experiments.
4. Animal are used in Science for:-Animal are used in Science for:-
UG teaching to learn physiological mechanismUG teaching to learn physiological mechanism
,anatomy and effect of various drugs on human,anatomy and effect of various drugs on human
body.body.
PG teaching to show effects of various drugs toPG teaching to show effects of various drugs to
find out the nature of unknown drug and it’sfind out the nature of unknown drug and it’s
effect or for bioassayeffect or for bioassay
In research to understand the working of body atIn research to understand the working of body at
disease and healthy statedisease and healthy state
In research to conduct screening for drugsIn research to conduct screening for drugs
bioassay and pre clinical testing of new drugsbioassay and pre clinical testing of new drugs
5. Animals are used to test possibilities that would beAnimals are used to test possibilities that would be
difficult or impossible to test using the target speciesdifficult or impossible to test using the target species
(humans).(humans).
It is mandatory to do extensive toxicological studies inIt is mandatory to do extensive toxicological studies in
animals before the drug gets approval for clinical trialsanimals before the drug gets approval for clinical trials
in humans.in humans.
““There is no doubt that the best test species for humansThere is no doubt that the best test species for humans
are humans. It's not possible to extrapolated animal dataare humans. It's not possible to extrapolated animal data
directly to humans you to inter species variation indirectly to humans you to inter species variation in
anatomy ,physiology and biochemistry”.anatomy ,physiology and biochemistry”.
6. Needs for Alternatives :-Needs for Alternatives :-
In Laboratory an animal may be….In Laboratory an animal may be….
1. Poisoned.1. Poisoned.
2. Deprived of food ,water and sleep.2. Deprived of food ,water and sleep.
3. Applied with skin and eye irritants.3. Applied with skin and eye irritants.
4. Subjected to physiological stress.4. Subjected to physiological stress.
5. Deliberately infected with disease.5. Deliberately infected with disease.
6. Brain damaged, paralysis, surgical mutilated.6. Brain damaged, paralysis, surgical mutilated.
7. Disadvantages of animal experiments :-Disadvantages of animal experiments :-
1.1. Pain, distress and unethical behavior toPain, distress and unethical behavior to
animals.animals.
2.2. Requirement of skilled manpower.Requirement of skilled manpower.
3.3. Time consuming protocol.Time consuming protocol.
4.4. High Cost.High Cost.
8. Laws and Regulations:-Laws and Regulations:-
YEARYEAR LAWLAW
19601960 Prevention of cruelty to animal act. (amend.1982).Prevention of cruelty to animal act. (amend.1982).
19641964 Committee for the purpose of control andCommittee for the purpose of control and
supervision of experimental animal (CPCSEA).supervision of experimental animal (CPCSEA).
19921992 Indian national science academy. “Guidelines forIndian national science academy. “Guidelines for
care and use of animals in scientific research.care and use of animals in scientific research.
20012001 Indian Council of medical Research (ICMR).Indian Council of medical Research (ICMR).
““Guidelines for use of laboratory animals inGuidelines for use of laboratory animals in
medical collages”.medical collages”.
20092009 MCI amendment to use alternatives to replaceMCI amendment to use alternatives to replace
animal experiments.animal experiments.
20132013 UGC guidelines for discontinue of animalUGC guidelines for discontinue of animal
experiments in zoology or life science in a phasedexperiments in zoology or life science in a phased
9. Modified use of animals:-Modified use of animals:-
Russel and Burch in 1959 proposed that ….Russel and Burch in 1959 proposed that ….
““If animals were to be used in experiments every effortIf animals were to be used in experiments every effort
should be made to replace them with non-sentientshould be made to replace them with non-sentient
alternatives”.alternatives”.
They developed the 3R strategy which includes:-They developed the 3R strategy which includes:-
1.1. Refinement:-Refinement:- refine experimental methods to decreaserefine experimental methods to decrease
unnecessary pain and trauma to animals.unnecessary pain and trauma to animals.
2.2. Reduction:-Reduction:- reduce the no. of animals used in thesereduce the no. of animals used in these
experiments.experiments.
3.3. Replacement:-Replacement:- replace the animal experiments .replace the animal experiments .
ex.Computar stimulation model , in-vitro method , cellex.Computar stimulation model , in-vitro method , cell
culture technique.culture technique.
10. 1.Refinement1.Refinement
Setting the earliest possible end point.Setting the earliest possible end point.
Using appropriate analgesics and anesthetics forUsing appropriate analgesics and anesthetics for
painful procedure.painful procedure.
Use proper handling technique for animals.Use proper handling technique for animals.
Adequate training prior to performingAdequate training prior to performing
experiment.experiment.
Ensure drug dose are correct and srug are notEnsure drug dose are correct and srug are not
expired.expired.
Performed surgeries and procedure aseptically toPerformed surgeries and procedure aseptically to
prevent infection.prevent infection.
11. 2.2.ReductionReduction
Perform pilot studies.Perform pilot studies.
Design studies to use animals as their ownDesign studies to use animals as their own
control. ex. Cross over study.control. ex. Cross over study.
Gather data for more than one experimentGather data for more than one experiment
concurrently.concurrently.
Consult with statistician and use minimum no. ofConsult with statistician and use minimum no. of
animals.animals.
Minimize variables such as disease , diet , stress,Minimize variables such as disease , diet , stress,
genetics.genetics.
Use appropriate species of animals.Use appropriate species of animals.
12. 3.Replacement3.Replacement
Substitution of insentient material in place ofSubstitution of insentient material in place of
conscious higher animals.conscious higher animals.
Could be relative or absolute.Could be relative or absolute.
Replace higher animals with lower animals .Replace higher animals with lower animals .
Replace live animals with dummies for teachingReplace live animals with dummies for teaching
and dissection purpose .and dissection purpose .
Use computer simulation and in-vitro methods .Use computer simulation and in-vitro methods .
Use cell culture and tissue culture.Use cell culture and tissue culture.
13. 1. In-Vitro Models:-1. In-Vitro Models:-
In-Vitro biomedical research entails theIn-Vitro biomedical research entails the
maintenance of organs, tissues (fragments ofmaintenance of organs, tissues (fragments of
organs and tissues), and cells outside the body.organs and tissues), and cells outside the body.
Can be grown as independent cell lines orCan be grown as independent cell lines or
preserve the architecture of the entire organ aspreserve the architecture of the entire organ as
organ culture and tissue culture.organ culture and tissue culture.
Stem cells are also used in in-vitro models.Stem cells are also used in in-vitro models.
14. Source of tissue for in-vitro methods:-Source of tissue for in-vitro methods:-
Avian – Chick embryosAvian – Chick embryos
Rodents – rats and mice (wild types and adult traRodents – rats and mice (wild types and adult tra
-nsgenic). Embryonic, post-natal and-nsgenic). Embryonic, post-natal and
and adult.and adult.
Human – 1. Neural progenitor cells from abortedHuman – 1. Neural progenitor cells from aborted
fetuses and stem cell line.fetuses and stem cell line.
2. cord blood derived stem cells.2. cord blood derived stem cells.
15. Types of in-vitro System:-Types of in-vitro System:-
Cell Culture
Cell Lines
Primary
culture
Organ
architecture
preserved
16. In-vitro methods :-In-vitro methods :-
In-vitro pyrogen test .In-vitro pyrogen test .
Embryonic stem cell test .Embryonic stem cell test .
Local lymph node assay for skin sensitization.Local lymph node assay for skin sensitization.
Clinical skin patch test on human volunteers.Clinical skin patch test on human volunteers.
Neutral red uptake assay.Neutral red uptake assay.
Carcinogenicity test.Carcinogenicity test.
Acute toxicity test.Acute toxicity test.
Repeated dose toxicity test.Repeated dose toxicity test.
Development neurotoxicity test.Development neurotoxicity test.
17. 2.Microorganism based model:-2.Microorganism based model:-
Tetrahymena pyriformis – a ciliate protozoanTetrahymena pyriformis – a ciliate protozoan
being used to study the effects of anesthetics onbeing used to study the effects of anesthetics on
metabolism.metabolism.
Salmonella typhimurium – bacteria used inSalmonella typhimurium – bacteria used in
mechanistic studies in genetics as well as themechanistic studies in genetics as well as the
Ames mutagenicity test.Ames mutagenicity test.
18. 3.3.In-chemico testing :-In-chemico testing :-
The toxic potential of substances can sometimes beThe toxic potential of substances can sometimes be
detected using relatively simple chemistry baseddetected using relatively simple chemistry based
methods and not requiring human cells.methods and not requiring human cells.
ex. HPLCex. HPLC
Direct peptide relativity assay – used to assess whether aDirect peptide relativity assay – used to assess whether a
chemical or cosmetics will cause allergy.chemical or cosmetics will cause allergy.
The test works by mimicking a key step in theThe test works by mimicking a key step in the
development of allergies – the binding of proteinsdevelopment of allergies – the binding of proteins
found in the skin to substance.found in the skin to substance.
If proteins bind to the substance them its very unlikelyIf proteins bind to the substance them its very unlikely
that it will cause an allergic reaction.that it will cause an allergic reaction.
19. 4.4.In-silico Models :-In-silico Models :-
Computer aided molecular drug design.Computer aided molecular drug design.
Quantitative structure activity relationships.Quantitative structure activity relationships.
Computer assisted learning.Computer assisted learning.
Computer or mathematical analysis.Computer or mathematical analysis.
Microfludic chipsMicrofludic chips
DNA chips.DNA chips.
Organ on chipOrgan on chip
Human on chipHuman on chip
20. 5.5.Computer assisted learning (CAL) :-Computer assisted learning (CAL) :-
CAL deals with a range of software packagesCAL deals with a range of software packages
which simulate the animal experiments.which simulate the animal experiments.
Two software's are currently used in india.Two software's are currently used in india.
1.1. Ephram – developed by JIPMER, India.Ephram – developed by JIPMER, India.
2.2. X-cologyX-cology
21. ExpharmExpharm
Contains programs on……..Contains programs on……..
1.1. Effects of drugs on rabbit eye.Effects of drugs on rabbit eye.
2.2. Bio assay of histamine using guinea pig ileum.Bio assay of histamine using guinea pig ileum.
3.3. Effects of drug on frog heart.Effects of drug on frog heart.
4.4. Effects of drugs on dog blood pressure and HREffects of drugs on dog blood pressure and HR
5.5. Effects of drugs on cillary movement of frogEffects of drugs on cillary movement of frog
esophagus.esophagus.
The user can conduct experiment and collect data.The user can conduct experiment and collect data.
Each program can be run in two modes :-Each program can be run in two modes :-
1.1. Tutorial modeTutorial mode
2.2. Examination modeExamination mode
22. THE ENDTHE END
DEEPAK JOSHIDEEPAK JOSHI
M.PHARMAM.PHARMA
(PHARMACOLOGY)(PHARMACOLOGY)