A Novel Hypothesis as to the Origin of Autism: An Alteration in Biological Water Dynamics Disrupts Proton-Coupled Electron Transfer and the Organizing Function of CNS Fractones
Oral presentation and abstract by Robert M. Davidson, M.D. Ph.D. FAIS at the International Conference on Brain Disorders and Therapeutics, London, United Kingdom, August 25th 2015, titled "A Novel Hypothesis as to the Origin of Autism: An Alteration in Biological Water Dynamics Disrupts Proton-Coupled Electron Transfer and the Organizing Function of CNS Fractones"
This study assessed the impact of urban intensification on water quality and microbial communities in canals in Suzhou, China. Samples were taken from sites with high, medium, and low population densities in both summer and spring. Nutrient levels, leaf litter breakdown, and microbial abundance varied between intensity areas and seasons. Microbial communities showed elevated nutrients and microbial levels in highly urbanized sites. Organic matter broke down faster in summer. Sequencing of microbial communities is ongoing but results so far indicate urbanization alters water quality and microbes.
Expanding the window - the past, present, and future of Minnesota's waterScott St. George
Nearly all decisions about water in Minnesota relate either directly or indirectly to data collected by the state’s hydrological observing network. Because most gauges were installed in early 20th century, as a whole the network provides us with roughly a 100-year ‘window’ to estimate flood risks, develop worst-case scenarios for drought, and set maximum allowable withdrawals for aquifers. But when we rely exclusively on observations made during this relatively brief interval, we may inadvertently increase our exposure to hydrological ‘surprises’. In order to make sound decisions about water in Minnesota, we need to expand this window: into the past, drawing upon historical accounts and natural archives; and into the future, via projections from climate and hydrological models. By cultivating a broader perspective on hydrological variability and extremes across the state, we will be better prepared to ensure adequate water supplies and mitigate the impacts of future floods and droughts.
JacksonCreek_Evan_Juliana_Kristen_Adrienne_RebekahRebekah Short
The document summarizes a report card on the health of the Jackson Creek watershed in Peterborough, Ontario. It delineates the watershed area and notes that monitoring its health is important as it runs through an urban center. The report card evaluates the watershed based on 2016 data on factors like benthic invertebrates, phosphorus levels, and E. coli levels from sampling sites. It gives the watershed an overall grade of B for these measures but notes some areas could be improved. Maintaining the ecological integrity of the watershed requires involvement from various stakeholders and continued monitoring through annual report cards.
The study examined the effects of stream channelization ("cleaning") on nitrogen retention in three streams in central Pennsylvania. Preliminary results showed that channelized reaches allowed nitrogen atoms to travel much further (3.7 times) compared to unaltered reference reaches. The uptake velocity, which represents overall nitrogen demand, was on average 4.7 times higher in reference reaches. These differences were likely due to a lack of stored organic matter in channelized reaches, as indicated by lower ecosystem respiration rates. The results suggest that widespread stream cleaning could reduce nitrogen uptake and potentially increase nitrogen flux into Chesapeake Bay.
This document discusses paleoflood research along the Red River. It provides evidence that past floods along the river were larger than any observed in recorded history. Tree ring analysis reveals details about 3 extremely large floods that occurred in the 17th, 18th and 19th centuries. The tallest scars on trees from these events indicate water levels exceeded any modern floods by 1-3 meters. Studying past flood sizes and frequencies provides important information about the full range of Red River flood risk.
Pptx of slides for jones ray effect finalpatrons99
This document proposes a new unified theory of disease based on the Jones-Ray effect, which describes how certain salts affect the surface tension of water. It suggests that the Jones-Ray effect influences interfacial tensions in the body, which are important for processes like cell engulfment and signaling. Electron micrographs show how cholesterol sulfate can stabilize red blood cell membranes by reducing water stress at interfaces. The theory posits that membrane-bound water exhibits quantum properties and waves that resonate between exclusion zones and cell membranes. Maintaining optimal interfacial tensions may be clinically significant for conditions like membrane stability and neuroreceptor function.
In many settings, trees growing on floodplains provide an important source of indirect evidence that may be used to infer the occurrence, extent, and magnitude of floods prior to direct observations. That evidence may take several forms, including external scars caused by abrasion or impact from floating debris, anatomical changes within the annual growth increment following prolonged stem or root inundation, or tilting or uprooting due to the hydraulic pressure of floodwaters. Likely the most useful characteristic of paleoflood studies based on floodplain trees is their relatively high temporal resolution and dating accuracy compared to most other methods. Dendrochronological methods can routinely date past floods to the year of their occurrence and, in rare cases, can estimate the timing of floods that occur during the growing season to within two weeks. This high degree of chronological control, which is surpassed only by that provided by direct observation or instrumentation, can be used to determine whether floods in separate watersheds were synchronous or offset by several years and test hypotheses that suppose linkages between extreme floods and specific forcing mechanisms. Furthermore, the wide geographic distribution of tree species with dateable rings combined with the broad suite of methods available to examine interconnections between floods and tree growth allow this style of paleoflood hydrology to be applied to many settings that are not suitable for techniques that depend on geological evidence. Future paleoflood research involving tree rings will need to strike a balance between improving our understanding of the biological and fluvial processes that link tree growth to past events, and providing answers to questions about flood dynamics and hazards that are needed to safeguard people and property from future floods.
Expecting the unexpected: The relevance of old floods to modern hydrologyScott St. George
This document discusses the importance of understanding past floods to properly assess modern flood risks. It provides examples of paleoflood research along rivers like the Red River of the North, which uncovered major floods in 1826 and around 700 years ago that exceeded any floods measured by modern instrumentation. Understanding a river's full history through both recorded data and geological evidence of past floods is essential for floodplain management, development planning, and infrastructure design to mitigate future flood risks.
This study assessed the impact of urban intensification on water quality and microbial communities in canals in Suzhou, China. Samples were taken from sites with high, medium, and low population densities in both summer and spring. Nutrient levels, leaf litter breakdown, and microbial abundance varied between intensity areas and seasons. Microbial communities showed elevated nutrients and microbial levels in highly urbanized sites. Organic matter broke down faster in summer. Sequencing of microbial communities is ongoing but results so far indicate urbanization alters water quality and microbes.
Expanding the window - the past, present, and future of Minnesota's waterScott St. George
Nearly all decisions about water in Minnesota relate either directly or indirectly to data collected by the state’s hydrological observing network. Because most gauges were installed in early 20th century, as a whole the network provides us with roughly a 100-year ‘window’ to estimate flood risks, develop worst-case scenarios for drought, and set maximum allowable withdrawals for aquifers. But when we rely exclusively on observations made during this relatively brief interval, we may inadvertently increase our exposure to hydrological ‘surprises’. In order to make sound decisions about water in Minnesota, we need to expand this window: into the past, drawing upon historical accounts and natural archives; and into the future, via projections from climate and hydrological models. By cultivating a broader perspective on hydrological variability and extremes across the state, we will be better prepared to ensure adequate water supplies and mitigate the impacts of future floods and droughts.
JacksonCreek_Evan_Juliana_Kristen_Adrienne_RebekahRebekah Short
The document summarizes a report card on the health of the Jackson Creek watershed in Peterborough, Ontario. It delineates the watershed area and notes that monitoring its health is important as it runs through an urban center. The report card evaluates the watershed based on 2016 data on factors like benthic invertebrates, phosphorus levels, and E. coli levels from sampling sites. It gives the watershed an overall grade of B for these measures but notes some areas could be improved. Maintaining the ecological integrity of the watershed requires involvement from various stakeholders and continued monitoring through annual report cards.
The study examined the effects of stream channelization ("cleaning") on nitrogen retention in three streams in central Pennsylvania. Preliminary results showed that channelized reaches allowed nitrogen atoms to travel much further (3.7 times) compared to unaltered reference reaches. The uptake velocity, which represents overall nitrogen demand, was on average 4.7 times higher in reference reaches. These differences were likely due to a lack of stored organic matter in channelized reaches, as indicated by lower ecosystem respiration rates. The results suggest that widespread stream cleaning could reduce nitrogen uptake and potentially increase nitrogen flux into Chesapeake Bay.
This document discusses paleoflood research along the Red River. It provides evidence that past floods along the river were larger than any observed in recorded history. Tree ring analysis reveals details about 3 extremely large floods that occurred in the 17th, 18th and 19th centuries. The tallest scars on trees from these events indicate water levels exceeded any modern floods by 1-3 meters. Studying past flood sizes and frequencies provides important information about the full range of Red River flood risk.
Pptx of slides for jones ray effect finalpatrons99
This document proposes a new unified theory of disease based on the Jones-Ray effect, which describes how certain salts affect the surface tension of water. It suggests that the Jones-Ray effect influences interfacial tensions in the body, which are important for processes like cell engulfment and signaling. Electron micrographs show how cholesterol sulfate can stabilize red blood cell membranes by reducing water stress at interfaces. The theory posits that membrane-bound water exhibits quantum properties and waves that resonate between exclusion zones and cell membranes. Maintaining optimal interfacial tensions may be clinically significant for conditions like membrane stability and neuroreceptor function.
In many settings, trees growing on floodplains provide an important source of indirect evidence that may be used to infer the occurrence, extent, and magnitude of floods prior to direct observations. That evidence may take several forms, including external scars caused by abrasion or impact from floating debris, anatomical changes within the annual growth increment following prolonged stem or root inundation, or tilting or uprooting due to the hydraulic pressure of floodwaters. Likely the most useful characteristic of paleoflood studies based on floodplain trees is their relatively high temporal resolution and dating accuracy compared to most other methods. Dendrochronological methods can routinely date past floods to the year of their occurrence and, in rare cases, can estimate the timing of floods that occur during the growing season to within two weeks. This high degree of chronological control, which is surpassed only by that provided by direct observation or instrumentation, can be used to determine whether floods in separate watersheds were synchronous or offset by several years and test hypotheses that suppose linkages between extreme floods and specific forcing mechanisms. Furthermore, the wide geographic distribution of tree species with dateable rings combined with the broad suite of methods available to examine interconnections between floods and tree growth allow this style of paleoflood hydrology to be applied to many settings that are not suitable for techniques that depend on geological evidence. Future paleoflood research involving tree rings will need to strike a balance between improving our understanding of the biological and fluvial processes that link tree growth to past events, and providing answers to questions about flood dynamics and hazards that are needed to safeguard people and property from future floods.
Expecting the unexpected: The relevance of old floods to modern hydrologyScott St. George
This document discusses the importance of understanding past floods to properly assess modern flood risks. It provides examples of paleoflood research along rivers like the Red River of the North, which uncovered major floods in 1826 and around 700 years ago that exceeded any floods measured by modern instrumentation. Understanding a river's full history through both recorded data and geological evidence of past floods is essential for floodplain management, development planning, and infrastructure design to mitigate future flood risks.
Tree rings have been used to reconstruct streamflow in the Colorado River basin over centuries. This revealed that severe multi-year droughts were more common than shown in the instrumental record. A 1,000-year drought reconstruction for western North America also indicated droughts could last decades. Tree rings have additionally helped develop the North American Drought Atlas which provides drought context for historical events and shows Asian monsoon variability over centuries.
Laura Fields-Sommers conducted a study assessing the effects of riverbank inducement on groundwater quality in a shallow aquifer in southeastern Wisconsin. She found that 1) cation exchange capacity in wells near the river was depleted, likely due to mixing of river water and groundwater, 2) river water was influenced by wastewater treatment plant effluent as shown through isotopic signatures, and 3) bacterial sequencing revealed genetic markers from human waste at some sampling sites, indicating sewage contamination of the river and groundwater.
The document summarizes a PhD study investigating the impacts of invasive riparian plants on juvenile salmonids in low order streams. The study monitored 24 sites across 6 rivers, with paired control and treatment sites where invasive plant coverage exceeded 50%. Over two years, the study collected biotic and abiotic samples to assess differences between native and invaded sites, including fish surveys, invertebrate samples, and vegetation surveys. Preliminary analysis found invasive cover had no effect on overall fish biomass or diet, but positively influenced salmon density and negatively influenced trout density, possibly due to differences in habitat preferences between the two species under conditions of bank instability from plant dieback. Further analysis of samples is ongoing to better understand impacts on a fine scale
Flood rings: Paleoflood evidence in tree-ring anatomyScott St. George
In low-gradient, low energy rivers, forms of tree-ring evidence such as impact scars or stem deformation do not provide useful evidence of past floods. In this talk, I explain the strengths and limitations of wood anatomy as tools in in paleoflood hydrology.
Don’t call it a comeback: Studying ancient floods to prepare for future hazardsScott St. George
How long do we need to watch a river before its behavior holds no more surprises? In this country, instrumental measurements of river stage and discharge stretch back a century or more, but this observed history still provides only a rough guide to the risks of future extreme floods. In this lecture, I’ll outline how paleoflood hydrology expands our perspective on river history by combining historical, botanical, and geological evidence of earlier (and ofttimes unknown) floods. And I’ll explain how we can interpret those physical clues left behind by ancient floods to improve hazard assessments for at-risk communities, support decisions about flood infrastructure, and investigate the long-term effects of climate or land-use changes on flooding. Because what has happened before can happen again, most everyone near a river would profit by keeping a longer memory of old floods.
1) Researchers analyzed microorganisms trapped in fluid inclusions in ancient halite samples to learn about past microbial communities and seawater chemistry.
2) They found 11% genetic similarity between microbes in modern brine and those trapped in halite millions of years ago, showing some microbes can survive long-term.
3) Analysis of fluid inclusions indicated seawater was calcium-rich in the early Carboniferous period, and the researchers hope to document shifts in chemistry over time.
Large-scale dendrochronology and low-frequency climate variabilityScott St. George
Large-scale low-frequency variability has emerged as a priority for climate research, but instrumental observations are not long enough to characterize this behavior or gage its impacts on dependent geophysical or ecological systems. As the leading source of high-resolution paleoclimate information in the middle- and high-latitudes, tree rings are essential to understand low-frequency variability prior to the instrumental period. But even though tree rings possess several advantages as climate proxies, like other natural archives they also have their own particular impediments. In this lecture, Dr. St. George will describe the structure and characteristics of the Northern Hemisphere tree-ring width network, and outline how the fingerprint of decadal and multidecadal climate variability encoded within ancient trees varies across the hemisphere.
Fall poster-Seasonal Variation of the Geochemistry and the EffectsMichelle C Bennett
1) Microbial communities in the Fraser River vary seasonally and correlate with environmental factors like temperature, turbidity, ammonia, and silica. Certain bacteria like Sphingobacteria and Flavobacteria indicate seasonal changes and are more abundant in summer months.
2) The document analyzes samples from the Fraser River and its tributaries to understand the link between microbial diversity, river geochemistry, and transported particles. Economic activities in the Fraser River Basin include forestry, fishing, agriculture, and more.
3) Analysis found the most abundant bacteria phyla responded to changes in temperature, ammonia, turbidity, and silica. Sphingobacteria were more abundant in summer months compared to Flavobacteria
This document discusses an investigation into how aquatic life responds to changes in river channels. It is supervised by three people and focuses on the spatial and temporal scales that influence biological and physical composition in rivers. The spatial scales that most influence composition are the reach scale at 40%, followed by the site scale at 20%, with the catchment and network scales also impacting composition at 20% and 10% respectively.
This study evaluated the impact of pumping fresh water into Biscayne Bay near the Deering Estate to address rising salinity levels. Macroalgae and seagrass communities were monitored as indicators of ecological change. After fresh water flooding, sites separated into distinct pre- and post-flooding clusters. Relative frequencies suggested a shift toward more brackish-tolerant species and decline in marine species. Nutrient analysis found higher nitrogen levels in macroalgae like Ulva ohnoi and in seagrasses near fresh water sources, indicating flooding increased nutrient availability. Continued monitoring will compare results to previous studies to further assess flooding impacts.
Aquaphotomics uses near-infrared spectroscopy to analyze the absorbance pattern of water and how it changes with solutes. The water absorbance pattern contains information about the solvent environment and changes in the pattern indicate changes in solutes. This dynamic spectroscopy technique reveals information about aqueous and biological systems through analysis of water structure.
This document discusses an experiment investigating how changing the counterion in AOT reverse micelles affects the properties of water trapped in the micelle core. Specifically, it looks at how substituting sodium for other cations like lithium, potassium, or ammonium impacts the infrared spectroscopy frequency of isocyanate ion probes in the water core at different water concentrations (w0). The results show that using NH4(AOT) or K(AOT) makes the water bonding in the reverse micelles more similar to bulk water, while sodium causes an anomalous blue shift indicating more polar water.
This document lists numerous publications and presentations by C.M. Buswell relating to their research on biofilms and pathogens like Campylobacter jejuni. It includes 14 peer-reviewed publications, many focusing on C. jejuni survival in aquatic biofilms and the role of coaggregation. It also lists over 20 conference presentations and poster presentations given between 1995-2004 on similar topics related to biofilms, C. jejuni, and coaggregation.
This document summarizes a student research project that studied the evolution and biogeography of stickleback fish armor traits across four watersheds on Vancouver Island, Canada. The students analyzed stickleback populations to explore how gene flow, natural selection, and population loss shape the geographic distribution of body armor morphs. They found variation in armor traits across watersheds that seems to be influenced by factors like gene flow patterns, introduced predators, and lake chemistry. Their results provide insights into how local ecological conditions and evolutionary processes interact to drive phenotypic diversity in these freshwater fish populations.
This document discusses water contamination from a land clearing site. Photos show the land clearing site from a distance, erosion downstream from the site, and potentially contaminated water supply. The contamination appears to have come from runoff during land clearing work.
Ryan Krysl has over 10 years of experience in biology research and environmental compliance. He holds a Master's degree in Biology from the University of Nebraska - Omaha and a Bachelor's degree from the University of South Dakota. Currently he works as an Environmental Staff Scientist for the Kansas City Board of Public Utilities, where he conducts inspections and manages environmental regulations and projects. Previously he has held research positions studying the effects of agricultural chemicals on aquatic life and worked as a graduate assistant conducting molecular biology techniques and data analysis.
Noah, Joseph, And High-Resolution PaleoclimatologyScott St. George
In 1968, Benoit Mandelbrot and James Wallis published an article titled ‘Noah, Joseph, and operational Hydrology’ in the journal Water Resources Research. In it, they argued that hydrological models of the day were not able to estimate the true risk of extreme floods or prolonged drought, and that rare hydrological events were much more common than usually assumed.
In this lecture, I’ll review how high-resolution paleoenvironmental archives can help us judge more accurately the risks posed by the ‘Noah’- and ‘Joseph’-style events described by Mandelbrot and Wallis. I’ll give particular emphasis to the environmental information recovered from the rings of ancient trees, and explain how dendrochronology (tree-ring research) has been used to redefine the ‘flood of record’, test potential avenues for long-lead climate predictions, and gage the performance of state-of-the-art climate models.
1) The document provides a historical overview of perspectives on autism from 1820 to 2020, highlighting several key milestones.
2) In the 1940s, Kanner and Asperger independently identified and named autism as a distinct syndrome, describing features like insistence on sameness and isolated skills.
3) In the 1960s, research shifted away from viewing autism as caused by parenting and toward studying cognitive and information processing deficits, applying new psychological models.
Dissertation on environmental pollution and global warming 27 08-2013Patricia Rodrigues
This document provides an introduction to environmental pollution and global warming. It defines key terms like environment, environmental pollutant, and environmental pollution. It then discusses the genesis of environmental problems, noting that both developing and developed countries contribute to pollution in different ways. In India specifically, issues like river pollution, deforestation, industrial waste, and noise pollution are discussed. The document also examines the relationship between religious and cultural practices in India and environmental pollution. It argues that traditions like celebrating festivals by bursting firecrackers, disposing of waste in rivers, and using loudspeakers contribute to pollution problems.
Developing a Hypothesis and Title for your ExperimentJeremy Mularella
The document discusses how to develop a hypothesis and title for a scientific experiment. It explains that a hypothesis predicts the relationship between an independent variable and dependent variable in an "if...then" statement. Several examples are provided of hypotheses and experiment titles that follow the proper format of stating the effect of an independent variable on a dependent variable. Guidance is given on key terms like "effect" versus "affect" and how to clearly define the independent and dependent variables.
This document discusses the importance and characteristics of formulating hypotheses in quantitative research studies. It defines a hypothesis as a tentative prediction about the relationship between two or more variables that can be tested. Good hypotheses should be researchable, stated in declarative form, define the relationship between variables, and be testable. Hypotheses can follow from theoretical frameworks and allow theories to be evaluated indirectly through hypothesis testing. The document provides examples of directional and nondirectional, simple and complex, research and null hypotheses.
Tree rings have been used to reconstruct streamflow in the Colorado River basin over centuries. This revealed that severe multi-year droughts were more common than shown in the instrumental record. A 1,000-year drought reconstruction for western North America also indicated droughts could last decades. Tree rings have additionally helped develop the North American Drought Atlas which provides drought context for historical events and shows Asian monsoon variability over centuries.
Laura Fields-Sommers conducted a study assessing the effects of riverbank inducement on groundwater quality in a shallow aquifer in southeastern Wisconsin. She found that 1) cation exchange capacity in wells near the river was depleted, likely due to mixing of river water and groundwater, 2) river water was influenced by wastewater treatment plant effluent as shown through isotopic signatures, and 3) bacterial sequencing revealed genetic markers from human waste at some sampling sites, indicating sewage contamination of the river and groundwater.
The document summarizes a PhD study investigating the impacts of invasive riparian plants on juvenile salmonids in low order streams. The study monitored 24 sites across 6 rivers, with paired control and treatment sites where invasive plant coverage exceeded 50%. Over two years, the study collected biotic and abiotic samples to assess differences between native and invaded sites, including fish surveys, invertebrate samples, and vegetation surveys. Preliminary analysis found invasive cover had no effect on overall fish biomass or diet, but positively influenced salmon density and negatively influenced trout density, possibly due to differences in habitat preferences between the two species under conditions of bank instability from plant dieback. Further analysis of samples is ongoing to better understand impacts on a fine scale
Flood rings: Paleoflood evidence in tree-ring anatomyScott St. George
In low-gradient, low energy rivers, forms of tree-ring evidence such as impact scars or stem deformation do not provide useful evidence of past floods. In this talk, I explain the strengths and limitations of wood anatomy as tools in in paleoflood hydrology.
Don’t call it a comeback: Studying ancient floods to prepare for future hazardsScott St. George
How long do we need to watch a river before its behavior holds no more surprises? In this country, instrumental measurements of river stage and discharge stretch back a century or more, but this observed history still provides only a rough guide to the risks of future extreme floods. In this lecture, I’ll outline how paleoflood hydrology expands our perspective on river history by combining historical, botanical, and geological evidence of earlier (and ofttimes unknown) floods. And I’ll explain how we can interpret those physical clues left behind by ancient floods to improve hazard assessments for at-risk communities, support decisions about flood infrastructure, and investigate the long-term effects of climate or land-use changes on flooding. Because what has happened before can happen again, most everyone near a river would profit by keeping a longer memory of old floods.
1) Researchers analyzed microorganisms trapped in fluid inclusions in ancient halite samples to learn about past microbial communities and seawater chemistry.
2) They found 11% genetic similarity between microbes in modern brine and those trapped in halite millions of years ago, showing some microbes can survive long-term.
3) Analysis of fluid inclusions indicated seawater was calcium-rich in the early Carboniferous period, and the researchers hope to document shifts in chemistry over time.
Large-scale dendrochronology and low-frequency climate variabilityScott St. George
Large-scale low-frequency variability has emerged as a priority for climate research, but instrumental observations are not long enough to characterize this behavior or gage its impacts on dependent geophysical or ecological systems. As the leading source of high-resolution paleoclimate information in the middle- and high-latitudes, tree rings are essential to understand low-frequency variability prior to the instrumental period. But even though tree rings possess several advantages as climate proxies, like other natural archives they also have their own particular impediments. In this lecture, Dr. St. George will describe the structure and characteristics of the Northern Hemisphere tree-ring width network, and outline how the fingerprint of decadal and multidecadal climate variability encoded within ancient trees varies across the hemisphere.
Fall poster-Seasonal Variation of the Geochemistry and the EffectsMichelle C Bennett
1) Microbial communities in the Fraser River vary seasonally and correlate with environmental factors like temperature, turbidity, ammonia, and silica. Certain bacteria like Sphingobacteria and Flavobacteria indicate seasonal changes and are more abundant in summer months.
2) The document analyzes samples from the Fraser River and its tributaries to understand the link between microbial diversity, river geochemistry, and transported particles. Economic activities in the Fraser River Basin include forestry, fishing, agriculture, and more.
3) Analysis found the most abundant bacteria phyla responded to changes in temperature, ammonia, turbidity, and silica. Sphingobacteria were more abundant in summer months compared to Flavobacteria
This document discusses an investigation into how aquatic life responds to changes in river channels. It is supervised by three people and focuses on the spatial and temporal scales that influence biological and physical composition in rivers. The spatial scales that most influence composition are the reach scale at 40%, followed by the site scale at 20%, with the catchment and network scales also impacting composition at 20% and 10% respectively.
This study evaluated the impact of pumping fresh water into Biscayne Bay near the Deering Estate to address rising salinity levels. Macroalgae and seagrass communities were monitored as indicators of ecological change. After fresh water flooding, sites separated into distinct pre- and post-flooding clusters. Relative frequencies suggested a shift toward more brackish-tolerant species and decline in marine species. Nutrient analysis found higher nitrogen levels in macroalgae like Ulva ohnoi and in seagrasses near fresh water sources, indicating flooding increased nutrient availability. Continued monitoring will compare results to previous studies to further assess flooding impacts.
Aquaphotomics uses near-infrared spectroscopy to analyze the absorbance pattern of water and how it changes with solutes. The water absorbance pattern contains information about the solvent environment and changes in the pattern indicate changes in solutes. This dynamic spectroscopy technique reveals information about aqueous and biological systems through analysis of water structure.
This document discusses an experiment investigating how changing the counterion in AOT reverse micelles affects the properties of water trapped in the micelle core. Specifically, it looks at how substituting sodium for other cations like lithium, potassium, or ammonium impacts the infrared spectroscopy frequency of isocyanate ion probes in the water core at different water concentrations (w0). The results show that using NH4(AOT) or K(AOT) makes the water bonding in the reverse micelles more similar to bulk water, while sodium causes an anomalous blue shift indicating more polar water.
This document lists numerous publications and presentations by C.M. Buswell relating to their research on biofilms and pathogens like Campylobacter jejuni. It includes 14 peer-reviewed publications, many focusing on C. jejuni survival in aquatic biofilms and the role of coaggregation. It also lists over 20 conference presentations and poster presentations given between 1995-2004 on similar topics related to biofilms, C. jejuni, and coaggregation.
This document summarizes a student research project that studied the evolution and biogeography of stickleback fish armor traits across four watersheds on Vancouver Island, Canada. The students analyzed stickleback populations to explore how gene flow, natural selection, and population loss shape the geographic distribution of body armor morphs. They found variation in armor traits across watersheds that seems to be influenced by factors like gene flow patterns, introduced predators, and lake chemistry. Their results provide insights into how local ecological conditions and evolutionary processes interact to drive phenotypic diversity in these freshwater fish populations.
This document discusses water contamination from a land clearing site. Photos show the land clearing site from a distance, erosion downstream from the site, and potentially contaminated water supply. The contamination appears to have come from runoff during land clearing work.
Ryan Krysl has over 10 years of experience in biology research and environmental compliance. He holds a Master's degree in Biology from the University of Nebraska - Omaha and a Bachelor's degree from the University of South Dakota. Currently he works as an Environmental Staff Scientist for the Kansas City Board of Public Utilities, where he conducts inspections and manages environmental regulations and projects. Previously he has held research positions studying the effects of agricultural chemicals on aquatic life and worked as a graduate assistant conducting molecular biology techniques and data analysis.
Noah, Joseph, And High-Resolution PaleoclimatologyScott St. George
In 1968, Benoit Mandelbrot and James Wallis published an article titled ‘Noah, Joseph, and operational Hydrology’ in the journal Water Resources Research. In it, they argued that hydrological models of the day were not able to estimate the true risk of extreme floods or prolonged drought, and that rare hydrological events were much more common than usually assumed.
In this lecture, I’ll review how high-resolution paleoenvironmental archives can help us judge more accurately the risks posed by the ‘Noah’- and ‘Joseph’-style events described by Mandelbrot and Wallis. I’ll give particular emphasis to the environmental information recovered from the rings of ancient trees, and explain how dendrochronology (tree-ring research) has been used to redefine the ‘flood of record’, test potential avenues for long-lead climate predictions, and gage the performance of state-of-the-art climate models.
1) The document provides a historical overview of perspectives on autism from 1820 to 2020, highlighting several key milestones.
2) In the 1940s, Kanner and Asperger independently identified and named autism as a distinct syndrome, describing features like insistence on sameness and isolated skills.
3) In the 1960s, research shifted away from viewing autism as caused by parenting and toward studying cognitive and information processing deficits, applying new psychological models.
Dissertation on environmental pollution and global warming 27 08-2013Patricia Rodrigues
This document provides an introduction to environmental pollution and global warming. It defines key terms like environment, environmental pollutant, and environmental pollution. It then discusses the genesis of environmental problems, noting that both developing and developed countries contribute to pollution in different ways. In India specifically, issues like river pollution, deforestation, industrial waste, and noise pollution are discussed. The document also examines the relationship between religious and cultural practices in India and environmental pollution. It argues that traditions like celebrating festivals by bursting firecrackers, disposing of waste in rivers, and using loudspeakers contribute to pollution problems.
Developing a Hypothesis and Title for your ExperimentJeremy Mularella
The document discusses how to develop a hypothesis and title for a scientific experiment. It explains that a hypothesis predicts the relationship between an independent variable and dependent variable in an "if...then" statement. Several examples are provided of hypotheses and experiment titles that follow the proper format of stating the effect of an independent variable on a dependent variable. Guidance is given on key terms like "effect" versus "affect" and how to clearly define the independent and dependent variables.
This document discusses the importance and characteristics of formulating hypotheses in quantitative research studies. It defines a hypothesis as a tentative prediction about the relationship between two or more variables that can be tested. Good hypotheses should be researchable, stated in declarative form, define the relationship between variables, and be testable. Hypotheses can follow from theoretical frameworks and allow theories to be evaluated indirectly through hypothesis testing. The document provides examples of directional and nondirectional, simple and complex, research and null hypotheses.
This document provides an overview of hypotheses for a presentation. It begins with learning outcomes which are to explain the meaning and significance of hypotheses, identify types of hypotheses, and illustrate why hypotheses are needed.
The presentation will cover the scientific method, meaning and types of variables, characteristics of good hypotheses, categories of hypotheses including null and alternative, and how to form and test hypotheses. Hypotheses are defined as educated guesses that relate variables and guide research. They must be testable, falsifiable, and contribute to theory. Hypotheses can be categorized by their formulation as null or alternative, by direction as directional or non-directional, and by their derivation as inductive or deductive.
The document defines and discusses hypotheses in research contexts. It provides that a hypothesis is a formal, testable statement of the expected relationship between independent and dependent variables. The document outlines several definitions of a hypothesis provided by authors and discusses the key characteristics of a good hypothesis. It also differentiates between different types of hypotheses such as universal, existential, null, alternate, non-directional, directional, and research hypotheses. The purpose, components, and process of hypothesis making and testing are described.
This document provides an overview of hypothesis testing including:
- Defining null and alternative hypotheses
- Types of errors like Type I and Type II
- Test statistics and significance levels for comparing means, proportions, and standard deviations of one and two populations
- Examples are given for hypothesis tests on population means, proportions, and comparing two population means.
The document discusses hypothesis testing in research. It defines a hypothesis as a proposition that can be tested scientifically. The key points are:
- A hypothesis aims to explain a phenomenon and can be tested objectively. Common hypotheses compare two groups or variables.
- Statistical hypothesis testing involves a null hypothesis (H0) and alternative hypothesis (Ha). H0 is the initial assumption being tested, while Ha is what would be accepted if H0 is rejected.
- Type I errors incorrectly reject a true null hypothesis. Type II errors fail to reject a false null hypothesis. Hypothesis tests aim to control the probability of type I errors.
- The significance level is the probability of a type I error,
Presentation on Dependency Theory for PS 212 Culture and Politics in the Third World at the University of Kentucky, Summer 2007. Dr. Christopher S. Rice, Instructor.
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A Novel Hypothesis as to the Origin of Autism: An Alteration in Biological Water Dynamics Disrupts Proton-Coupled Electron Transfer and the Organizing Function of CNS Fractones
1. A Novel Hypothesis as to the Origin of Autism:
An Alteration in Biological Water Dynamics
Disrupts Proton-Coupled Electron Transfer and the
Organizing Function of CNS Fractones
Robert M. Davidson M.D. Ph.D. FAIS*
Ann Lauritzen, Stephanie Seneff,
Stephen D. Kette, Glyn Wainwright,
Anthony Samsel, and Sydney J. Bush
*Fellow, The American Institute of Stress
Physician and Medical Researcher, Kilgore, Texas, USA
Email: patrons99@yahoo.com
International Conference on Brain Disorders and Therapeutics
August 24-26, 2015 London, United Kingdom
1
2. OVERVIEW:
2
• THE GOAL: Apply a chemical biology, biophysical perspective to the
(a) pathophysiology and
(b) prevention of Autism, Schizophrenia, and Alzheimer’s
• THE HOPE: To one day find a cure, or preferably, a prevention
• Hans Selye: Most diseases are pluricausal, highly-stereotyped, and
supramolecular in origin.
• TODAY: Let’s focus on just 3 levels of biosemiotic organization:
SUBATOMIC MOLECULAR CELLULAR
4. There’s A New Game in Town! It’s Non-Linear!
• Chemical Biology is the gateway to Quantum Biology
• Medical schools need to teach new ways of looking at
(a) health at the molecular level
(b) underlying causes of diseases
• We’ve got to escape the trap of “linear thinking”: the “magic bullet” mentality
4
“A pill or shot for every malady”
5. A Novel Hypothesis:
The Origin of the Fractal Dimension
WE PROPOSE:
• Bipolarons represent hydrated electrons in low pH reductive environments
– Hydrated electrons represent the "fractal dimension”
– Redox potential and surface energy of water are pH-dependent
-- pH dependent speciation of e(aq’s) modulates their ability to undergo Bose-Fröhlich-Einstein
condensation and might play a role in quantum consciousness
• CNS fractones need a "universal sulfurylation factor” and 2-O-sulfate-L-ascorbate radical may
be the universal sulfurylation factor, facilitated magnetohydrodynamically by hydrated electrons to
supply sulfate to HSPGs
• Fractones are functionally maintained as stem cell "niches" via autocatalytic radical
initiation, propagation, and comproportionation of the ascorbate radical
5
6. Conceptual Genesis of Our Hypothesis:
The Origin of The Fractal Dimension
6
(1) Pang, X.F. The conductivity properties of protons in ice and mechanism of magnetization of liquid water. Eur.
Phys. J. B 2006, 49, 5–23.
(2) Davidson, R.M.; Lauritzen, A.; Seneff, S. Biological Water Dynamics and Entropy: A Biophysical Origin of
Cancer and Other Diseases. Entropy 2013, 15, 3822-3876.
(3) Men, Z., Fang, W., Li, Z., Sun, C., Li, Z. and Wang, X. (2015). Hydrated-electron resonance enhancement O-H
stretching vibration of water hexamer at air-water interface. Optics letters, 40, 1434-1437.
7. Our Proposal for pH Dependent e(aq) Speciation
7
Mizuse, K. and Fujii, A. (2013). Characterization of a Solvent-Separated Ion-Radical Pair in Cationized Water Networks: Infrared
Photodissociation and Ar-Attachment Experiments for Water Cluster Radical Cations (H2O)n+ (n = 3–8). The Journal of Physical
Chemistry A, 117, 929-938.
Larsen, R.E., Glover, W.J. and Schwartz, B.J. (2010). Does the hydrated electron occupy a cavity? Science, 329, 65-69.
8. Our Proposal for a Universal Sulfurylation Factor
8
Takebayashi, J., Kaji, H., Ichiyama, K., Makino, K., Gohda, E., Yamamoto, I. and Tai, A. (2007). Inhibition of free radical-induced erythrocyte
hemolysis by 2-O-substituted ascorbic acid derivatives. Free Radical Biology and Medicine, 43, 1156-1164.
Verlangieri, A.J. and Mumma, R.O. (1973). In vivo sulfation of cholesterol by ascorbic acid 2-sulfate. Atherosclerosis, 17, 37-48.
Cabral, J., Haake, P. and Kessler, K. (1998). Rearrangement of 3-Acyl Derivatives of L-Ascorbic Acid. Journal of Carbohydrate Chemistry, 17, 1321-1329.
WE PROPOSE: 2-O-sulfate L-ascorbate radical plays a central role in the "universal
nonspecific mesenchymal reaction", which has also been referred to as the Sanarelli-
Shwartzman phenomenon.
9. A Vortex of Hexagons
from the Cassini Space Probe
9
10. Our Hypothesis: Dynamical Bidirectional 3-D Vortices
of Interfacial Water at the Interphase
10
Pieranski, P., Sotta, P., Rohe, D. and Imperor-Clerc, M. (2000). Devil's staircase-type faceting
of a cubic lyotropic liquid crystal. Physical review letters, 84, 2409-2412.
A drop of soapy molecules and water can form
a rigid gel crystal that displays more facets
than any crystal ever observed–
a phenomenon called ‘the Devil’s staircase.’
http://physics.aps.org/story/v5/st10
11. Our Hypothesis: Chiral Paramagnetic Induction
Homeotropic Alignment of Water at the Interphase
• Ascorbate radical may act as inducer, nidus, or seed to chirally and
paramagnetically-align biological water at the interphase [homeotropic
enrichment]. Consider a chirally and paramagnetically-aligned liquid-
crystalline matrix or "plasma" of spin-correlated radical pairs
• Interfacial water provides the physical basis for (a) chemotaxis and
allostery, (b) a non-metabolic “fuel” source for molecular motors, (c) the
ability to overcome intra- and extracellular "crowding" and thermal diffusion
(kT) problems, and (d) uniquely trapping and transducing ELF EM energy
from environment into charge separation and negentropy (-∆S)
• Exogenous Interfacial Water Stress (EIWS) is the disruption of normal
interfacial tension between water and biomacromolecules in vivo by
exogenous agents such as the Al³+ cation --- represents the root cause or
initiator of inflammation and disease (Seneff et al 2015).
11
12. Long-Range Attractive Intermolecular Forces
• NANOASSOCIATES: Konovalov has shown that sunlight/water/hydrophilic surface
sufficient to form nanoassociates of water (Konovalov et al 2014);
• NEGENTROPY: configurational (conformational and vibrational) entropy loss
• THIXOTROPY: pH-dependent gel-like “memory” of water (Verdel and Bukovec
2013)
• PERCOLATION THRESHOLD: the entropic, hydration, lipid, protein, and sulfur
requirements for biological activity (Brovchenko et al 2006; Davidson et al 2013)
• SELF-ORDERED CRITICALITY: Biophysical quantum coherent equipoise of the
CNS is disrupted by EIWS; molecular recognition, chemotaxis, and allostery are
lost in the presence of EIWS (Pal and Zewail 2004; Shaw et al 2014)
12
13. Fractals, Anisotropy, Hydrophobic Effect
• Fractal— geometrical scaling law from math and physics
• Anisotropy— order, alignment, e.g. birefringence on intravital
polarized light microscopy; e.g. loss of fractional anisotropy
on DTI-MRI precedes anatomic and behavioral
signs/symptoms of neurological disease (SDAT, ASD, MS,
schizophrenia)
• Hydrophobic Effect— Lum-Chandler-Weeks theory (Lum et al
1997) “size matters”; "order at the edge of chaos"; Bertholet's
classical experiments; Martin Gruebele's seminal "stretching
water" experiment
13
14. Exogenous Interfacial Water Stress Theory
• Exogenous Interfacial Water Stress (EIWS) is the root cause or initiator leading to
inflammation and disease; EIWS is the initial common pathway to all disease (Davidson and
Seneff 2012; Davidson et al 2013; Seneff et al 2015)
CAVEAT: The surface energy and redox potential of water are pH-dependent. IWS subsumes
redox stress, i.e. the superficial grand potential subsumes redox potential
• Al³+ aquo cation is a prime example of EIWS; Myelin is a preferred target of Aluminium
toxicity (Verstraetten et al 1997); It’s time to add Gadolinium to the list of EIWS sources
(McDonald et al 2015); both Gadolinium and Aluminum are neurotropic
• EIWS precedes inflammation and immune activation (MIA and IL-6) which precedes Autism,
Macrophagic MyoFasciitis (MMF), Schizophrenia, SDAT, MS, SLE, etc,
• EIWS is supramolecular, biophysically-pleiotropic, pluricausal, and highly-stereotyped
• EIWS theory provides a biophysical explanation for synergistic toxicity
14
15. The Cytoskeleton, Defined
• Dynamical combination of hydrated actin microfilaments, microtubules, and fibrils:
(a) exquisitely sensitive to EIWS,
(b) provides a biomechanical conduit for energy transfer between the intracellular and
extracellular matrix via proton-coupled electron transfer (PCET)
• F-Actin has been shown to be associated with hypermobile water by Makoto Suzuki’s group using
dielectric spectroscopy (Kabir , et al 2003; Wazawa, et al 2011)
• According to the TOFT: the default cytoskeletal state is cytoproliferative and motile
(Sonnenschein and Soto, The Society of Cells, 1999; Soto and Sonnenschein 2011)
• Under the EIWS theory: the cytoskeletal state is controlled by gradients of interfacial water
tension at the interphase (Davidson, et al 2013); interfacial water stress subsumes redox stress
• Under the EIWS theory: interfacial water exists in a metastable, dynamical state of equipoise in
a liquid-crystalline matrix, wherein the protein aggregates comprising the cytoskeleton are
modulated by and substantially “slaved” to the dynamics of interfacial water
15
16. Fractones, Defined:
• Fractones have been referred to as stem cell “niches” and they are thought to regulate
cytoskeletal assembly and organize the ECM of the heart, gut, brain, and bone
marrow/RES. They have complex cytoarchitectures consisting of stem cells, progenitor
cells, supporting cells, and laminin-rich basement membranes (Hochman-Mendez et al
2014).
• Frederic Mercier and his associates have studied neural stem cell niches and described
fractones as:
“particulate extracellular matrix structures that I previously characterized in both the
developing and adult brain”. --- F. Mercier (personal statement)
“In the neural stem cell niche of the adult brain, I have demonstrated that fractone-
associated heparan sulfate proteoglycans serve as captors and activators of growth
factors to regulate neural stem cell proliferation.”
Mercier, F., Kitasako, J.T. and Hatton, G.I. (2002). Anatomy of the brain neurogenic zones revisited: fractones and the
fibroblast/macrophage network. J Comp Neurol, 451, 170-188.
16
17. Polymerized Laminin is Hexagonal !
Is PolyLM the structural basis for CNS Fractones?
• Fractal nature and hexagonal symmetry of polylaminin (polyLM) has been noted on
confocal fluorescence microscopy (CFM), scanning electron microscopy (SEM), and
atomic force microscopy (AFM) (Hochman-Mendez et al 2014)
• “SEM and AFM analyses revealed that the seed unit of polyLM was a flat polygon formed
in solution whereas the seed structure of LM was highly heterogeneous, intercalating rod-
like, spherical and spread lamellar deposits.” (emphasis added)
• “A search for the Hausdorff dimension in images of the two matrices showed that polyLM,
but not LM, presented fractal dimensions…” (emphasis added)
• “…the intrinsic fractal nature of polymerized laminin can be the structural basis for the
fractal-like organization of basement membranes in the neurogenic niches of the central
nervous system.” (emphasis added)
17
18. Structural Basis for the Organizing Functions
of CNS Fractones
WE PROPOSE:
Hydrated electrons, specifically bipolarons in low pH, reductive environments, represent the fractal
dimension. (Davidson et al 2013). Support for this proposal was found by Hochman-Mendez et al
2014.
“…key signaling properties of laminin were preserved and even augmented after the acid-induced
assemblage, which was demonstrated mainly for neurons [8], but also for other cells types as glial
[9] and thyroid cells [19].” (emphasis added)
• In low pH, reductive environments, fractones and hydrated electrons (bipolarons) provide:
(a) a water-mediated physical basis for chemotaxis and allostery of signalling molecules,
molecular motors, and,
(b) a coherent bidirectional electromagnetic "connection" or "conduit“ for PCET between the
ECM, fractones, caveolae of the plasma membranes, the cytoskeleton, and mitochondria.
18
19. Fractone-Plasma Membrane-Cytoskeletal “Connection”
WE PROPOSE:
A coherent liquid-crystalline “connection” between the subatomic and cellular (mesoscopic)
levels of biosemiotic organization consisting of:
(a) Josephson-like QM tunneling effects, (b) hydrated electrons, (c) PCET, (d) mitochondrial
membranes, (e) cytoskeletal actin, (f) lipid rafts (caveolae) of plasma membrances, (g)
integrins, and (h) laminin-rich basement membranes
• Cell morphology is highly-correlated with biomechanical, functional, hemorheological
properties of cells
• Cellular polarization occurs when cell energy flows, either in centrifugal or centripetal
direction, in the resting and proliferative states, respectively
• WHEREAS, the default cytoproliferative and motile state is typically kept in check, at
times, EIWS disinhibits and “unleashes” the default state
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20. CNS Fractones Need Sulfur
• GAGs and HSPGs need a UNIVERSAL SULFURYLATION
FACTOR, e.g. 2-O-sulfate-L-ascorbate radical might
preempt and supercede PAPS/SULTS
• CNS fractones need BOTH ascorbate and sulfur
• HSPGs have been shown to be low in sulfur at autopsy of
CNS fractones in Autism
Corley et al (2012); Mercier et al (2012); Meyza et al (2012); Pearson et al (2013); Mercier
et al (2011)
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21. Autism as a Sulfate and/or Ascorbate Deficiency
• MOUSE MODEL of Autism and a GUINEA PIG MODEL of Scurvy
• Jones-Ray Effect: biphasic concentration dependent surface enhancement; Riddick
Effect: biphasic concentration dependent zeta potential enhancement
• A New Triad in Classical Autism: abnormal RBC shape, oxidative damage, β-Actin (Ciccoli
et al 2013); EIWS is very likely to precede the Autism Triad; Sulfate Deficiency may be a
cause of EIWS
• A Plausible scenario: Sulfate deficiency EIWS Autism Triad Autism phenotype
• SEM studies of RBCs by Bleau, et al (1975) suggest that Ch-S supplementation might
reverse the RBC morphological and hemorheological abnormalities in Autism
• Both sulfate and ascorbate deficiency may represent synergistic consumptive sequelae of
EIWS
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22. Multiple Scales of Time and Space
• “There’s plenty of room at the bottom” – Richard Feynman (1959); both Feynman and Irving
Langmuir observed “like-likes-like” physical and biophysical behavior, a phenomenon familiar to
colloid chemists. Our blood can be thought of as a colloidal suspension that flows.
• Medical schools need to teach a multidisciplinary vocabulary to accommodate the “new” science
of biophysics, soft matter physics, chemical biology, redox biochemistry, liquid-crystal chemistry,
magnetohydrodynamics, and quantum biology: this is where we will find the cipher…the Rosetta
Stone
EIWS – Exogenous Interfacial Water Stress
TOFT – Tissue Organization Field Theory
SCRPM - [electron] spin coupled radical pair mechanism
EPR - electron paramagnetic resonance
PCET - proton coupled electron transfer
CPET - concerted proton electron transfer
NQMT - nuclear quantum mechanical tunneling
KIE - kinetic isotope effect, mass independent KIEs
HFI - hyperfine interaction (electron spin - nuclear spin interaction)
DTI-MRI - diffusion tensor imaging magnetic resonance functional imaging; includes calculated
measures of [fractional] anisotropy and diffusion OF WATER !
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23. Summary
• [e (aq)] •+ [Asc 2-S] •ˉ sGAGs/HSPGs/F-actin filaments Autism/RBC phenotype
• Hydrated electrons represent the physical basis for the “fractal dimension”
• We propose pH dependent speciation of e(aq’s) as a requirement for quantum consciousness
• Autism and Other Brain Disorders may represent Sulfate and/or Ascorbate Deficiency Syndromes
which sensitizes via EIWS to inflammation, microvascular ischemic, hemorheologic, and
thrombohemorrhagic phenomena
• Autism may represent an EIWS-induced loss of self-ordered criticality and molecular recognition
which leads to maternal immune activation and autoimmunity
• Autism may be result from EIWS-induced disturbance of a CNS fractone function, anywhere along
the cytoskeletal energy “connection” between mitochondria and CNS fractones
• The Autism Triad may be explained in terms of sulfate and ascorbate deficiency, both of which may
represent synergistic consumptive sequelae of EIWS
• Fractones of the brain, heart, gut, and bone marrow require a “universal sulfurylation factor” and
we have proposed the structure for such a factor, i.e. the 2-O-sulfate-L-ascorbate radical
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25. Conceptual Genesis of Hypothesis:
The Fractal Dimension
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Pang, X.F. The conductivity properties of protons in ice and mechanism of magnetization
of liquid water. Eur. Phys. J. B 2006, 49, 5–23.
Davidson, R.M.; Lauritzen, A.; Seneff, S. Biological Water Dynamics and Entropy: A
Biophysical Origin of Cancer and Other Diseases. Entropy 2013, 15, 3822-3876.
26. Our Proposal for a Universal Sulfurylation Factor
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Takebayashi, J., Kaji, H., Ichiyama, K., Makino, K., Gohda, E., Yamamoto, I. and Tai, A. (2007). Inhibition of free radical-induced
erythrocyte hemolysis by 2-O-substituted ascorbic acid derivatives. Free Radical Biology and Medicine, 43, 1156-1164.
Verlangieri, A.J. and Mumma, R.O. (1973). In vivo sulfation of cholesterol by ascorbic acid 2-sulfate. Atherosclerosis, 17, 37-48.
Cabral, J., Haake, P. and Kessler, K. (1998). Rearrangement of 3-Acyl Derivatives of L-Ascorbic Acid. Journal of Carbohydrate
Chemistry, 17, 1321-1329.
27. Our Speculation: Frequency-Locked Vortices?
• A cubic lyotropic liquid-crystal of surfactant and water demonstrated an
infinite number of facets to suggest “Devil’s Staircase” frequency-locking
(Pieransky et al 2000)
• Are hydrated electrons involved?
• Are spin-correlated, frequency-locked, hydrated electrons involved? In
vivo?
• Is a frequency-encoded hydrated electron the singularity at the apex of
vortices? In vivo?
Pieranski, P., Sotta, P., Rohe, D. and Imperor-Clerc, M. (2000). Devil's staircase-type faceting of a cubic lyotropic
liquid crystal. Physical review letters, 84, 2409-2412.
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28. A Novel Hypothesis:
The Origin of the Fractal Dimension
WE PROPOSE THE FOLLOWING:
• Protomeric/electromeric cyclic hexamer radical-cations of water (bipolarons) represent hydrated electrons,
and vehicles for PCET in low pH, reductive environments, in vivo
• Hydrated electrons, i.e. electrons solvated by cyclic water hexamers, represent the “fractal dimension”, the
singularity at the apex of 3-D dynamical vortices of biological water, both in vivo and in vitro.
• The need by HSPGs of CNS fractones for sulfate, implies that CNS fractones have a need for a “universal
sulfurylation factor”
• 2-O-sulfate-L-ascorbate radical may be a universal sulfurylation factor which is attracted by water-mediated
chemotaxis to CNS fractone, faciliated magnetohydrodynamically by dynamical vortices of hydrated
electrons, so as to affect the sulfurylation of HSPGs
• 2-O-sulfate-L-ascorbate radical has a kinetically-transient lifetime sufficient to act as a post-translational
“sulfurylation factor” of GAGs and HSPGs
• In liquid-crystalline lyotropic phases of biological water at the interphase, the transulfurylation transition-state
would be stabilized by on-water heterogeneous catalysis, PCET, and by facile formation of resonance-
stabilized cyclic trigonal bipyramidal intermediates which can undergo Berry pseudorotation.
• Our proposal might be repeated through-out life to maintain functionality of fractones as stem cell “niches” via
autocatalytic radical initiation, propagation, and comproportionation of the ascorbate radical.
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