1) The document describes an artificial inoculation protocol for Maize Lethal Necrosis (MLN) to achieve uniform disease pressure for screening maize germplasm. 2) It involves collecting and purifying the causal viruses SCMV and MCMV from infected fields, amplifying the inoculum in a screen house, then preparing and applying the mixed viral inoculum to seedlings in the field using motorized sprayers. 3) Disease severity is rated on a 1-5 scale and data on agronomic traits and yield is collected to evaluate the maize lines for MLN resistance.