The living plant cell
What is the main differences between plant cell and animal cell??
Cell wall: Formed of cellulose.
Chloroplast: Responsible for photosynthesis.
Vacuole: much larger in plant cells, store any nutrients and waste products .
content
Cultivation and preparation of crude drugs
1- Cultivation
2- Collection
3- Preservation of plant material (Drying, stabilization and fermentation)
4- Packing
5- Storage
6- Grinding of crude drugs
7- crude drug extraction
content
Important definitions
Crude drug description
Pharmacist should have a good knowledge of natural drugs (why)
Nomenclature of drugs (Origin of the crude drug)
Classification of drugs for study
Colchicum - Anti Rheumatic agent | Cultivation | Description | Chemical Const...Chetan Prakash
This presentation provides knowledge about Colchicum, its description, collection& cultivation, Morphological characters, microscopical characters, powder characters, chemical constituents, chemical tests and its uses. This is an assignment in the subject Pharmacognosy & Phytochemistry, IIIrd B.Pharm
content
Cultivation and preparation of crude drugs
1- Cultivation
2- Collection
3- Preservation of plant material (Drying, stabilization and fermentation)
4- Packing
5- Storage
6- Grinding of crude drugs
7- crude drug extraction
content
Important definitions
Crude drug description
Pharmacist should have a good knowledge of natural drugs (why)
Nomenclature of drugs (Origin of the crude drug)
Classification of drugs for study
Colchicum - Anti Rheumatic agent | Cultivation | Description | Chemical Const...Chetan Prakash
This presentation provides knowledge about Colchicum, its description, collection& cultivation, Morphological characters, microscopical characters, powder characters, chemical constituents, chemical tests and its uses. This is an assignment in the subject Pharmacognosy & Phytochemistry, IIIrd B.Pharm
Subterranean organs characters and function, Subterranean Stem, Rhizome, Types of rhizomes, Histology of Subterranean Stem, The tegumentary tissue, Roots, Types of roots, Histology of dicotyledon Roots, Main differences between roots and subterranean stems, Anomalous structure in subterranean organs, In subterranean stem, Rhubarb, star spots, in Roots, Aconite, stellate form, senega, keel, Jalap, tertiary cambia, Orizaba Jalap, Belladonna and Gentian.
Synonyms: Radix Ginseng, Shin-seng.
Part Used:
The roots of the cultivated perennial herbs of Panax quinquefolium (American ginseng) and of Panax schin seng (Chinese ginseng) (Fam. Araliaceae).Collected from 5-6 years old plants, in autumn, carefully cleaned and dried.
Subterranean organs characters and function, Subterranean Stem, Rhizome, Types of rhizomes, Histology of Subterranean Stem, The tegumentary tissue, Roots, Types of roots, Histology of dicotyledon Roots, Main differences between roots and subterranean stems, Anomalous structure in subterranean organs, In subterranean stem, Rhubarb, star spots, in Roots, Aconite, stellate form, senega, keel, Jalap, tertiary cambia, Orizaba Jalap, Belladonna and Gentian.
Synonyms: Radix Ginseng, Shin-seng.
Part Used:
The roots of the cultivated perennial herbs of Panax quinquefolium (American ginseng) and of Panax schin seng (Chinese ginseng) (Fam. Araliaceae).Collected from 5-6 years old plants, in autumn, carefully cleaned and dried.
slide1- introduction
slide2-Plant Tissue
Plant tissues are of two types :-
Meristematic tissue
Permanent tissue
slide3-Meristematic Tissue
Meristematic tissues continuously form a number of new cells and helps in growth and are generally made up live cells . Meristematic tissues are the group of cells that have the ability to divide. These tissues in a plant consist of small, densely packed cells that can keep dividing to form new cells. Meristems give rise to permanent tissues and have the following characteristics:
the cells are small,
the cells walls are thin,
cells have large nuclei,
vacuoles are absent or very small
there are no intercellular spaces.
Types of Meristematic Tissue
Apical Meristem:- Apical meristem is present on root apex, stem apex, leaf buds and flower buds. They are responsible for growth in length, i.e. primary growth.
Lateral Meristem: Lateral meristem is present along the side of the stem. They are responsible for growth in girth, i.e. secondary growth.
Intercalary Meristem: Intercalary meristem is present at the base of leaf or internodes. They are present on either side of the node.
slide4-Permanent Tissue [Plant Tissue]
Once the cells of meristematic tissue divide to a certain extent, they become specialized for a particular function. This process is called differentiation. Once differentiation is accomplished, the cells lose their capability to divide and the tissue becomes permanent tissue. Permanent tissues are of two types, simple permanent tissue and complex permanent tissue.
Permanent tissue gives support and are generally made up of dead cells . The cells of permanent tissues do not have the ability to divide. These cells are already differentiated in different tissue types and is now specialized to perform specific functions. They are subdivided into two groups, simple tissues consisting of cells which are more or less similar, e.g. epidermis, parenchyma, chlorenchyma, collenchyma, sclerenchyma and complex tissues consisting of different kinds of cells, e.g. xylem and phloem.
slide5-Parenchyma tissue
The cells of parenchyma have thin cell wall. They are loosely packed; with lot of intercellular spaces between them. Parenchyma makes the largest portion of a plant body. Parenchyma mainly works are packing material in plant parts. The main function of parenchyma is to provide support and to store food.
It is loosely packed and inter cellular spaces are there .
In aquatic plants , air is filled in parenchyma tissue , so they are called Arenchyma .
Parenchyma in which chlorophyll is present is called chlorenchyma .
slide6- Collenchyma tissue
In collenchyma tissue , the cells are generally elongated and are circular , oval or polygonal in cross- section. Cell wall is evenly thickened with cellulose at the corners . It is present on internodes of the plant . It is closely packed and intercellular spaces are generally absent. It is a living cell and vacuo
General Methods for Isolation of Terpene Hydrocarbons
1. Fractional Distillation:
• This usually takes place under vacuum or in an atmosphere of inert gas,?? as many hydrocarbons are sensitive to heat in atmospheric oxygen (product name? ? ?).
• This can carried out by gradual increase of the distillation temperature by (1-5oC) or (5-10 oC) according to the boiling point of components.
2. Preparation of the Corresponding Crystalline Additive Products “Adducts”:
Characteristic addition products are formed by N2O3 (Dinitrogen trioxide), N2O4, NOCl (Nitrosyl chloride) and NOBr to yield nitrosites, nitrosates, nitrosochlorides, and nitrosobromides, respectively.
content
Flavonoids (importance, Physiological role and Therapeutic uses).
Flavonoids biosynthesis, General Properties and Tests for identity.
Flavone Glycosides (Diosmin and Apiin)
Flavonol Glycosides (rutin and Quercetrin)
Flavanone Glycosides (Hesperidin)
Silymarin (flavonolignans)
Isoflavonoids (Genistein)
Coumarin Glycosides
content
Anthracene glycosides
mechanism of Action
Chemistry
Tests for Identification of anthracene glycosides
I- Anthraquinones (alizarin, aloe-emodin and rhein)
Metabolism of Diacerein (1-8 Diacetyl Rhein)
II- Anthranols and anthrones (Chrysarobin and Barbaloin)
III- Dianthrones (Sennosides A-D and Hypericin)
Definition
Glycosides are non-reducing organic compounds that on hydrolysis with acids or enzymes yield:
1- A sugar part (or glycone, formed of one or more sugar units).
2- A non-sugar part (or aglycone, also called genin).
content
The evaluation of drugs
Evaluation methods of the crude drugs
Chemistry of Crude Drugs
Primary Metabolites (starch, protein and fixed oil)
secondary Metabolites (glycosides, alkaloids and volatile oil)
content
Deterioration of Herbal Drugs
Primary Factors (Light, Moisture/ Humidity, Temperature and Air Oxidation)
Secondary Factors
Control Measures for Deterioration
Adulteration of Crude Drugs
Detection of Adulterants
Definitions “volatile oils”, “Ethereal oils” or Essential oils”
Volatile oils: Complex liquid mixtures of odoriferous compounds of varying chemical composition, which easily evaporate when exposed to air at room temperature, and which are used for either their specific therapeutic activity or their aroma.
Aromatherapy is a branch of complementary medicine, which depends on the use of aromatic plants, their extracts, mainly their essential oils to promote health, beauty and vitality.
بعض العلاقات... مثل القرابة والصداقة...
تشتمل على معاني جميلة... و روابط انسانيه مهمة...
ولكن البعض يتغيرون ... تغيرهم الاحداث ...
فالاحداث هي امتحانات لقوة طرفي اي معادلة تربط اي اثنين...
أستاذ جامعي يقع قتيلا في مؤتمر تقيمه كلية الحقوق... أثناء نقاش مع زميل له ...
على الرغم من ان القتيل كان شخص وفيا ومعطاء لكل المحيطين به...
لكن هذا لم يمنع يد خائنة وخسيسة من ان تقتله...
وتجعل من الخيانة والغدر مقابلا للوفاء... كما كانت من قبل جزاء لسنمار.
عندما يتلوث الدم.... ليس بالجراثيم وانما بآثام القلب وشروره ليضخ دما ملوثا بالحقد والكراهيه لينقله الى اقدام تسوقك الى جريمه والى يدا لتجعل من السلاح الذي تمسكه ادآة سوداء ورسالة من قلب مريض رسالة بالموت
عندما يتحول نجاحك الى مرض وعقده نفسيه كبيره لشخص لا يستطيع ان يلاحقه .....
عندما لا تفلح مؤمرات ذلك الشخص في افشالك.....
عندما يقرر انك من يقف في طريقه وانه يتوجب عليه ازاحتك من طريقه بأي وسيله وان كانت القتل..
عندها فقط بدأت المذبحه...
هاجس جال بخاطر رجل الاعمال الناجح حسن المصري بأن اذى ما سيلحق بابنه الوحيد خالد وان كارثة ما ستحدث لشركته...
وبدون مقدمات تحول الهاجس لسلسله من الجرائم البشعه والغامضه التي راح ضحيتها هو وكل الموجوديين بقصره ...
وفي لحظاته الاخيره ... وبدون ادنى تفكير حاول ان يحمي اغلى الناس في حياته ...ابنه ....من تلك اليد الاثمه...
لتكن تلك المكالمه ذات الكلمات القليله .... يبلغ فيها خالد ابنه برساله...
رساله من قلب محب.... رساله مضمونها
ان الاب هو الملاذ الاخير
حصن يحمي ابنائه الى النهايه
ان الاب هو.....
الطابية الاخيره
Phenolic compounds
Precipitate animal proteins in hides and converting them into leather. “Tanning Industry”
Present in plants, ex. grapes, cranberry, hamamelis and tea leaves.
Opium is the air-dried milky exudate, or latex, obtained by incising the unripe capsules of the opium poppy Papaver somniferum (Papaveraceae).
the ripe capsule can contain up to 0.5% total alkaloids
Opium represents a much concentrated form and up to 25% of its mass is composed of alkaloids ( more than 40 alkaloids).
Structural features of Cinchona alkaloids
1- The basic skeleton of Cinchona alkaloids is Ruban-9-Ol.
2- Ruban nucleus is a combined skeleton formed from a quinoline ring attached to a quinuclidine ring (a bicyclic ring contain N) through methylene group.
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
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The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
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Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
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A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
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The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
2. Why we are studying botany?
Why we are studying plant tissues ?
1- Studying secrets of the drug factory ( plant cell ) to
obtain
A-the active constituents in maximum concentration
B- the drug in optimum quality to ensure the highest
activity.
Medicinal plants Dr. Mostafa Hegazy
3. 2- useful for other
pharmacognosy important
subjects as tissue culture and
biotechnology ex. Taxol gene
identification and transfer into
Saccharomyces cerevisiae.
3- Quality control (QC)
purposes to prevent adulteration
of valuable drugs with toxic or
less active plants.
Medicinal plants Dr. Mostafa Hegazy
4. The living plant cell
What is the main differences between plant
cell and animal cell??
Cell wall: Formed of cellulose.
Chloroplast: Responsible for photosynthesis.
Vacuole: much larger in plant cells, store any
nutrients and waste products .
5. Plant Tissues
Tissues are collections of cells (one or
more types) that work together.
There are several types of tissues in
plants, Cellular composition of each
tissue varies depending on its function .
6. Meristematic tissues (Growth Tissues)
Undifferentiated
•Have the ability to continue cell division
and differentiation for the life of the
plant.
• composed from small cells with dense
cytoplasm and proportionately large
nuclei.
Permanent
•Do not change into other kind of tissues.
•Retain their structure and functional
characteristics throughout life.
7. meristematic
tissues
Classification on basis of
position
Apical meristem
Located at tip of stems and
roots which responsible for
Elongation of roots and
shoots.
Intercalary meristem
in stem internodes
Lateral Meristem
Secondary growth in Most
trees, shrubs, and some
herbs
Cork cambium(Phellogen)
Produces cork cells and
cork parenchyma
Vascular cambium
Produces secondary
vascular tissue (xylem and
phloem)
Classification on basis of
function
Protoderm
forms epidermis
Procambium
forms primary vascular
tissue (xylem, phloem)
Ground Meristem
differentiates into ground
tissue (cortex, pith)
9. 1- Epidermal tissue: (epi, upon;
derma, skin)
- Continuous layer of cells
covering surface of plant.
-coated by a waterproof, waxy [a
fatty substance] cutin (Cuticle) to
prevent dehydration.
- control gas movement through
epidermal pores called stomata.
- Some epidermal cells have
epidermal hairs (trichomes).
10. - Stomata
- The guard cells are movable
- Function of stomata: osmoregulation
and regulation of gas exchange.
Water pores:
Are open stomata with immovable guard
cells. They function to expel water
excreted into the intercellular spaces.
11. TYPES OF STOMATA
The surrounding cells are of varying number,
e.g. Digitalis leaves
1) Anomocytic stomata or Ranunculaceous
The stoma surrounded by usually 3 or more subsidiary
cells one of which smaller than the other, e.g.
Solanaceous leaves (Belladonna, Datura, Hyoscyamus)
2) Anisocytic stomata or Cruciferous
(unequal-celled type)
The stoma surrounded by 2 subsidiary cells
perpendicular to the osteole e.g. Mentha leaf.
3) Diacytic stomata or Caryophyllaceous
(Cross-celled type)
The stoma surrounded by 2 or more subsidiary cells, 2
of which are parallel to osteole. e.g. Senna leaf, Coca
leaf.
4) paracytic stomata or Rubiaceous
12. Trichomes or Hairs
Glandular Hairs
( Head and stalk)
NonGlandular Hairs or Covering
Trichomes
Trichomes or Hairs
Glandular Hairs
( Head and stalk)
NonGlandular Hairs or Covering
Trichomes
Head
Stalk
13. Glandular
hairs
Unicellular
Not common
multicellular
Multicellular
head
Multicellular
stalk
Unbranched
stalk
Uniseriate
stalk
1 row
Biseriate stalk 2 rows
Biseriate head e.g.
compositae hair
Pluriseriate stalk 3
or more rows e.g.
shaggy hair of
cannabis
Branched stalk e.g
branched hair in
Hyoscyamus muticus
Unicellular stalk
With
1- bicellular head
in digitalis
2- ovoid or club
shape multicellular
head ( clavate hair
) in Solanaceae
3- short stalk with
8 radiating cells
head ( labiaceous
hair ) in mentha
Unicellular
head
Unicellular
stalk e.g.
Digitalis
Multicellular
uniseriate
stalk e.g.
Lavender and
Belladona
14. Nonglandular hairs
Unicellular
Unbranche
d ex. senna
Branched
ex. cruciferae
multicellular
Unbranched
Uniseriate
1 row ex. Belladonna
and digitalis
Biseriate
2 rows ex. Twin hair
Arnica flower And
calendula
Pluriseriate
3 or more rows ex.
Shaggy hair cumin
Branched
Simple ex. Body
ending with 2
branches in tobacco
Other types
1-balanced hair or T-
shaped hair pyrethrum
2- stellate hair in Boldo
3-candelabra hair of
mullein
4- peltate hair of
cascarilla
15. Ground Tissues:
1-Parenchyma
Characters:
• Most common cell type.
• Shape: Spherical
• Thin cell wall, Has intercellular spaces and Living at maturity.
• Most parenchyma cells have the ability to differentiate
into other cell types under special conditions:
1- During repair and replacement of organs after injury.
2-Can resume meristematic activity to produce adventitious roots
and shoots.
Main functions :
- Photosynthesis - Storage.
16. 2- Collenchyma (Greek, (kolla-glue)
Characters:
• Thick walls (usually with uneven
thickness) with cellulose.
• No intercellular spaces
• Living at maturity.
• Function: flexible support.
• Occur in groups just beneath the
epidermis:
- Beneath cork (outer parts) in bark.
- At the midrib of leaf below and above
vascular bundle.
17. 3- Sclerenchyma: (Greek, skleros, hard; en = in; chin
pour (support cells).
• Characters: Thick walls with cellulose and lignin
to give strength and rigidity.
• Dead at functional maturity (Cannot increase in
length)
• Function: structural strength and support.
• Two types of sclerenchyma:
a. Fibers: which are tough, pliable, strong, elongated
cells with tapering ends.
b. Sclereids: or stone cells, in which cells are not
elongated but give strength and support.
18. Complex plant tissues
1- Xylem: (Greek, xylon - wood).
- Dead at functionally maturity
- - function: Xylem transport water and minerals from
roots
to rest of plant.
- • Comprises:
1) Tracheids:
- Long slender, tapered at the ends, with well developed
lignified walls.
- Less efficient at conducting water. • More like a fiber.
19. 2) Vessel elements:
(1) Annual
(2) Spiral
(3) Scalariform
(4) Reticulate
(5) Pitted vessel
3) Xylem fibers: Add strength to xylem system.
4) Xylem parenchyma:
Function in storage of water and foods.
20. 2- Phloem: (Greek, phloas - bark)
- function translocation of food from leaves to
rest of plant.
• Comprises:
a) Sieve-tube members
b) Phloem fibers
c) Phloem parenchyma: storage cells.
21. Items Xylem Phloem
Made of Dead cells Living cells
Cytoplasm ? None Cytoplasm lining
Tissue also has…. Fibers Companion cells
Cell wall
-Thickness
-Material
Thick
Lignin (rigid )
Thin
Cellulose
Transports….. Water and Minerals Food
Carried to… Leaves Growing parts and Storage
organs
Permeability Impermeable Permeable
Direction of flow Upwards Up and Down
22. 3 -Periderm (Cork)
• Characters: Protective tissue/Replaces epidermis
in stems and roots (woody plants) that have
continuous secondary growth.
• Cork is not living at maturity.
The cell walls are highly suberized (suberin is a fatty
substance which covers lignified primary cell wall).
• Comprises: a) Cork tissue (phellem), b) Cork
cambium (phellogen) and c) Parenchyma
(phelloderm).
• Functions:
1) Protects against mechanical injuries and
excessive loss of internal moisture.
2) Gas exchange between the air and the interior
of the stem.
Cork
23. Cell Wall Chemical Impregnations:
(Latten. cutis – skin) Fatty substance on outer surface of the epidermal
cell walls, where it forms a layer known as cuticle.
A. Cutin
lignin is the most abundant plant polymer. Responsible for strength of
wood. The three common monolignols: paracoumaryl alcohol (1),
coniferyl alcohol (2) and sinapyl alcohol (3)
B. Lignin
Fatty material found in the cell walls of cork tissues and the Casparian
strips of the endodermis.
Suberin functions as a physical barrier, preventing water loss,
protection against pathogens and in wound healing process in plants
C. Suberin
Polymer of polysaccharides
D. Hemicellulose
Component in the cell walls of fungal cell walls, 6 carbon sugar on to
which a nitrogen containing group has been added.
E. Chitin
24. SECRETORY TISSUES
1-Secretory cells
2- Secretory cavities or sacs or internal Glands.
According to die method of formation they may be:
a- Schizogenous: Developed by die separation of the
cells thus enclosing a cavity which then becomes
enlarged and lined by secretary epithelial cells.
b- Lysigenous: Developed by the breaking down of
the cells, forming a cavity, not lined by a definite
secretory epithelium..
c- Schizo-Lysigenous: The cavity develops at first as
schizogenously but, later on, increases in size and
changed to Lysigenous by the breaking down of
bounding cells.
25. 3- Secretory Ducts or Canals:
These are tube like structures containing and
producing secretions. They may extend through
the whole length of the organ. They usually
produce volatile oil or oleo-resin.
4- Laticiferous structures :
cells or vessels containing secretion called latex