DNA recombinant technology involves manipulating genes in organisms. It uses restriction enzymes to cut DNA at specific sites, vectors to carry foreign DNA, and host organisms for transformation. The key steps are:
1) Isolating the gene of interest from a library or by synthesis
2) Inserting the gene into a vector like a plasmid using enzymes and ligation
3) Transforming the vector into a host organism through physical or chemical methods
4) Selecting transformed host cells that contain the recombinant DNA
5) Analyzing the new genes and proteins produced by the host.
Recombinant dna technology and DNA sequencinganiqaatta1
title: recombinant DNA technology and DNA sequencing
this lect will cover the pcr, isolation of DNA, detection of DNA and DNA manipulation joining DNA together. this is very important and it is required in research of every field especially medical related field.
Recombinant dna technology and DNA sequencinganiqaatta1
title: recombinant DNA technology and DNA sequencing
this lect will cover the pcr, isolation of DNA, detection of DNA and DNA manipulation joining DNA together. this is very important and it is required in research of every field especially medical related field.
Techniques Used in Recombinant DNA TechnologyMandeep Singh
This assignment cum Infographic presents you with vital knowledge about recombinant dna technology, its scope, its goals & objectives & the techniques uesd in it. I have referred & combined essential information from from various journals, infographics,essays, published papers of eminent researchers & scientists in this
A recombinant DNA molecule is produced by joining together two or more DNA segments usually originating from two different organisms.
More Specifically, a recombinant DNA molecule is a vector into which desired DNA fragment has been inserted to enable its cloning in an appropriate host.
Recombinant DNA molecules are produced with one of the following objectives:
1. To obtain large number of copies of specific DNA fragments.
2. Large scale production of the protein encoded by the gene.
3. Integration of the desired DNA fragment into target organism where it expresses itself.
Drought tolerant-genetically modified plants:
Present abiotic stress is a major challenge in our quest for sustainable food production as these may reduce the potential yields by 70% in crop plants
Of all abiotic stress, drought is regarded as the most damaging
Transgenic plants carrying genes for abiotic stress tolerance are being developed for water stress management
Conventional breeding approaches, involving inter specific and inter generic hybridizations and mutagenesis have been limited success.
Major problems have been the complexity of drought tolerance & low genetic yield components under drought conditions.
Unlike conventional plant breeding there is no need of repeated back crossing
Gene pyramiding or gene stacking through co-transformation of different genes with similar effects can be achieved.
Study of cloning vectors and recombinant dna technologySteffi Thomas
Study of cloning vectors, restriction endonuclease and DNA ligase, Recombinant DNA technology, Application of genetic engineering in medicine, Application of rDNA technology and genetic engineering in the production of interferons, Vaccines-hepatitis-B, Hormones-Insulin, Brief introduction to PCR
in this topic we will discuss the following contents
Introduction.
Cloning.
Discovery.
Molecules need in rDNA technology.
Enzymes.
Vectors.
Procedure or steps involves in rDNA technology.
Application of rDNA technology.
Advantages and disadvantages.
Techniques Used in Recombinant DNA TechnologyMandeep Singh
This assignment cum Infographic presents you with vital knowledge about recombinant dna technology, its scope, its goals & objectives & the techniques uesd in it. I have referred & combined essential information from from various journals, infographics,essays, published papers of eminent researchers & scientists in this
A recombinant DNA molecule is produced by joining together two or more DNA segments usually originating from two different organisms.
More Specifically, a recombinant DNA molecule is a vector into which desired DNA fragment has been inserted to enable its cloning in an appropriate host.
Recombinant DNA molecules are produced with one of the following objectives:
1. To obtain large number of copies of specific DNA fragments.
2. Large scale production of the protein encoded by the gene.
3. Integration of the desired DNA fragment into target organism where it expresses itself.
Drought tolerant-genetically modified plants:
Present abiotic stress is a major challenge in our quest for sustainable food production as these may reduce the potential yields by 70% in crop plants
Of all abiotic stress, drought is regarded as the most damaging
Transgenic plants carrying genes for abiotic stress tolerance are being developed for water stress management
Conventional breeding approaches, involving inter specific and inter generic hybridizations and mutagenesis have been limited success.
Major problems have been the complexity of drought tolerance & low genetic yield components under drought conditions.
Unlike conventional plant breeding there is no need of repeated back crossing
Gene pyramiding or gene stacking through co-transformation of different genes with similar effects can be achieved.
Study of cloning vectors and recombinant dna technologySteffi Thomas
Study of cloning vectors, restriction endonuclease and DNA ligase, Recombinant DNA technology, Application of genetic engineering in medicine, Application of rDNA technology and genetic engineering in the production of interferons, Vaccines-hepatitis-B, Hormones-Insulin, Brief introduction to PCR
in this topic we will discuss the following contents
Introduction.
Cloning.
Discovery.
Molecules need in rDNA technology.
Enzymes.
Vectors.
Procedure or steps involves in rDNA technology.
Application of rDNA technology.
Advantages and disadvantages.
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
1. DNA Recombinant Technology
Advance Metabolic Engginering
(Course Code 157609
Sahri Yanti
Student ID: 10832910
Ph.D Program
Applied of Chemistry Departement
Chaoyang University of Technology, Taiwan
3. DefinitionRecombinan DNA
Tecnology
Genetic engineering
Experimental techniques to manipulation of
The genes in an organism
Molecular cloning and transformation
Involves the scientific alteration of the structure of genetic
material in a living organism
Order to produce new species or bio-chemicals
The development and application of scientific methods, procedures, and
technologies that permit direct manipulation of genetic
Scarce biological product by introducing DNA isolated from
animals or plants into bacteria and then harvesting the product
from a bacterial colony
Identifiation, isolation and Insertion of gene interest into a vector
4. GENE, DNA & RNA
DNA, RNA, Diferent funtion and structur of DNA vs RNA
5. ■ Gene and DNA are the two genetic terminologies mainly
used to determine the characteristics of a living thing
■ In general, a gene is a short section of DNA and DNA-
Deoxyribonucleic acid is a molecule which carries the genetic
instructions or the hereditary materials
DNA
Genes
Chromosomes
Cell & Nucleus
Gene DNA
Genes are the DNA
stretches
which are encoded for
different proteins
DNA is a chemical which
stores. the genetic
information of an
organism
Regulates the traits of an
organism
Regulates the gene
regulation.
A small stretches of
DNA.
A long chain
polynucleotide.
Genes are made up of
either DNA
or RNA.
DNA is made up of
simpler monomer units
called nucleotides
Are coded with heredity
information.
Encodes the genetic
instructions.
6. There is another type of genetic material found in cells and
viruses known as ribonucleic acid (RNA).
The genetic information stored in DNA is then transcribed
into RNA, and the details in RNA are then translated for the
synthesis of the protein (nucleoprotein)
DNA & RNA fungtion
DNA RNA
Replication process:
Transferring the
genetic information
from one cell to its
daughters
and from one
generation to the next
Equal distribution of
DNA during the cell
division
Mutations: The
changes which occur
during the DNA
sequences
Transcription:
Cellular Metabolism
DNA Fingerprinting
Gene Therapy
Facilitate the
translation of DNA
into proteins
Functions as an
adapter molecule in
protein synthesis
Serves as a
messenger between
the DNA and the
ribosomes
They are the carrier
of genetic
information in all
living cells
Promotes the
ribosomes to choose
the right amino acid
which is required in
building up of new
proteins in the body.
7. DNA RNA
Deoxyriobosa as a suger Ribosa as a sugar
4 nitrogen bases in DNA:
Adenine, Guanine,
Cytosine, and Thymine
that pair together)
A—T ; C— G
4 nitrogen bases in RNA, A,G,C,
and U that pair together)
A—U ;C—G
DNA is DOUBLE
STRANDED, cannot
leave the nucleus. DNA
is tightly wound into
chromosomes. it is
exactly the same in each
cell. Portions of DNA are
called genes.
RNA is SINGLE STRANDED and
does not have to stay in the
nucleus. RNA not found in
chromosomes because it does
not carry the genetic code,
however it can read the DNA
code and take the information out
of the nucleus
DNA & RNA structure
8. The different types of RNA are involved in a various cellular process
tRNA
The transfer RNA is
responsible for choosing
the correct protein or the
amino acids required by
the body in-turn helping
the ribosomes.
rRNA
The rRNA is the
component of the
ribosome and are
located within the in the
cytoplasm of a cell,
where ribosomes are
found.
The ribosomal RNA is
primarily involved in the
synthesis and
translation of mRNA
into proteins in all living
organisms.
mRNA
Responsible for carrying
the genetic material to
the ribosomes and
insists as to what kind
of proteins is required
by the body.
10. Tools of Recombinan DNA
• The enzymes which include the restriction endonucleases
• – help to cut DNA molecule from specific nitrogeneus base
(the polymerases)
• - help to synthesize DNA molecul and bind it (the ligases)
• They are two types, namely endonucleases and
exonucleases: The endonucleases is the restriction enzyme
which cut DNA molecule at interior side. The exonucleases
cut in exterior side of DNA and cut the nucleotides from the
ends of the DNA strands.
• The restriction endonucleases used in recombinant DNA
technology play a major role in determining the location at
which the desired gene is inserted into the vector genome.
Restriction
Enzymes
A recombinant DNA technology can be complete and achieved with the help of some elemental
tools. The different tools used for the purpose are: Restriction Enzymes, Vectors, Host organism.
11. ■ Restriction enzymes cut DNA molecules at specific locations
to produce restriction fragments with blunt ends or sticky
ends.
■ (a) Rsal restriction enzymes produce blunt-ended fragments
(b) EcoRI produces sticky ends by hanging at the 5 'end (c)
KpnI produces a sticky end by hanging at the 3' end.
12. • any DNA molecule that has the abylity to replicated inside the host which the
desiride gene has integrated for cloning
• Yeast cells, viruses, plasmids are the most commonly used vectors
• The vectors help in carrying and integrating the desired gene
• These form a very important part of the tools of recombinant DNA technology as
they are the ultimate vehicles that carry forward the desired gene into the host
organism
• To choose a suitable cloning vector for research, several things that must be
displayed are the size of the DNA fragment to be transferred
Cloning
Vectors
Tools of Recombinan DNA
■ Vector – DNA source which can replicate and is used to carry
foreign genes or DNA fragments.
■ Recombinant DNA – A vector that has taken up a foreign piece
of DNA.
A kind of host cell with contain vectors
are: transformed with alterated vector,
and with recombinan vector
13. ■ Overhanging sticky ends will complementarily
base pair, creating a recombinant DNA
molecule.
■ DNA ligase will seal the nick in the
phosphodiester backbone.
■ After cutting DNA with restriction enzymes, the
fragments can be separated on an agarose gel.
○ The smaller fragments will migrate
further than the longer fragments in an
electric field.
○ The bands are compared to standard
DNA of known sizes. This is often called
a DNA marker, or a DNA ladder.
14. • Host organism is the organism into which
the recombinant DNA is introduced.
• The host is the ultimate tool of
recombinant DNA technology which takes
in the vector engineered with the desired
DNA with the help of the enzymes.
• There are a number of ways in which this
recombinant DNA’s are inserted into the
host, namely – microinjection, biolistics or
gene gun, alternate cooling and heating,
use of calcium ions.
• Plants cells can also be transformed using
electroporation, which uses an electric
shock to make the cell membrane
permeable to plasmid DNA.
Competent
Host
Organism
Tools of Recombinan DNA
A gene gun uses biolistics to
insert DNA into plant tissue
16. •Getting gene interset from genomic library, cDMA
library, chemical syntesis of gene that knowing
subsquence
Identification and
isolation of gene
interest or DNA
fragment to be
cloned
•Cut plasmiid side by
Retriction enzyme
•Bind it by ligase
Introduction
suitable vector into
sutable organism or
host
(tranformation)
•Phiscal gene
tranfer
mehtode
•Chemical gene
transfer
methode
•DNA
imbibitions by
cell, tissues,
organs that call
Transformation
Insertion of
isolation gene into
suitable vector as
plasmid.
1
2&3
4
17. •3 kind of host cell: non
transformed,
transformed with
alterated vector, and
with recombinan vector.
•Can sellected by
antibiotic resisten in
selected medium, visible
characters, Assay for
biological activity, colony
hibriditation, blotting test
Selection the
transformened
host cell
Multiplication or
expression of
introduced gene
in the host
Analyse new
gene and protein
that pduce by
5
Cutting the gene at the recognition sites Polymerase chain reaction
Insertion of the desired recombinant DNA
into the host organism.