The aimed of current research to formulate herbal toothpaste utilizing plant extract like Neem leaves, Guava leaves, Cinnamon bark other ingredient are Camphor, Honey. The plant extract ingredient posses the anti-bacterial. The herbal toothpaste formulated which can satisfy all the required condition to keep the mouth fresh and prevent tooth decay by bacteria. The formulated herbal toothpaste compared with marketed preparation. Physical examination: Colour-greenish brown, smooth in nature, relative density-10.2, pH-8.2, Extrudability-90.37, spredability-Good and stable formulation. The anti-microbial evaluation against Staphylococcus aureus reveal that formulated herbal tooth paste exhibited notable activity with ZOI of 19.7 mm at MIC of 25μg/mL. the outcome of this research herbal toothpaste shows equal patronizing and engrossing passion over the marketed preparation it was consider after the comparing the marketed preparation(Colgate, Dabour Red, Dantkanti) with formulated herbal toothpaste. It has been good scope in future dental research and detal health of public.

1. Formulation and Evaluation of Herbal Toothpaste:
Compared with marketed preparation
Pavan Deshmukh1, Roshan Telrandhe2*, Mahendra Gunde3
Kamla Nehru College of Pharmacy, Butibori, Nagpur 441108, Maharashtra, India
ABSTRACT
The aimed of current research to formulate herbal toothpaste utilizing plant extract like Neem
leaves, Guava leaves, Cinnamon bark other ingredient are Camphor, Honey. The plant extract
ingredient posses the anti-bacterial. The herbal toothpaste formulated which can satisfy all the required
condition to keep the mouth fresh and prevent tooth decay by bacteria. The formulated herbal
toothpaste compared with marketed preparation. Physical examination: Colour-greenish brown, smooth
in nature, relative density-10.2, pH-8.2, Extrudability-90.37, spredability- Good and stable formulation.
The anti-microbial evaluation against Staphylococcus aureus reveal that formulated herbal tooth paste
exhibited notable activity with ZOI of 19.7 mm at MIC of 25μg/mL. the outcome of this research
herbal toothpaste shows equal patronizing and engrossing passion over the marketed preparation it was
consider after the comparing the marketed preparation(Colgate, Dabour Red, Dantkanti) with
formulated herbal toothpaste. It has been good scope in future dental research and detal health of
public.
MATERIAL AND METHOD
1. REFERENCES
2. Telrandhe R. Nanotechnology for cancer therapy: Recent developments. Eur J Pharm Med
Res. 2016;3(11): 284-294.
3. Telrandhe R, Mahapatra D K, Kamble M A. Bombax ceiba thorn extract mediated
synthesis of silver nanoparticles: Evaluation of anti-Staphylococcus aures activity. Int J
Pharm Drug Anal. 2017;5(9): 376-379.
4. Shende V, Telrandhe R. Formulation and evaluation of Tooth Gel from Aloe vera leaves
extract. Int J Pharm Drug Anal. 2017;5(10): 394-398.
5. Deshmukh P, Telrandhe R, Gunde M. Formulation and Evalua-tion of Herbal Toothpaste:
Compared With Maeketed Preparation. Int J Pharm Drug Anal. 2017;5(10): 406-410.
6. Telrandhe R, Deshmukh P, Gunde M. Formulation and Evalua-tion of Herbal Toothpaste:
Compared With Maeketed Preparation. Int J Pharm Drug Anal. 2017;5(10): 406-410.
7. Trivedi L, Telrandhe R, Dhabarde D. Differential spectrophotometric method for
estimation and validation of Verapamil in Tablet dosage form. Int J Pharm Drug Anal.
2017;5(11): 419-422.
8. Nanotechnology for Cancer therapy: Recent developments
https://www.researchgate.net/publication/321669196_Nanotechnology_for_Cancer_thera
py_Recent_developments
9. Nanoparticle of plant extract: A Novel approach for cancer therapy
https://www.researchgate.net/publication/321641952_Nanoparticle_of_plant_extract_A_
Novel_approach_for_cancer_therapy
10. Nanoparticle of plant extract: A Novel approach for cancer therapy
https://www.researchgate.net/publication/315654928_Nanoparticle_of_plant_extract_A_
Novel_approach_for_cancer_therapy
11. Bombax ceiba thorn extract mediated synthesis of silver nanoparticles: Evaluation of anti-
Staphylococcus aureus activity
12. https://www.researchgate.net/publication/321682787_Bombax_ceiba_thorn_extract_medi
ated_synthesis_of_silver_nanoparticles_Evaluation_of_anti-
Staphylococcus_aureus_activity
13. Formulation and Evaluation of Tooth Gel from Aloe vera leaves extract
Presented in NATIONAL CONFERENCE ;A Phytomedicine : A Novel
Approach For Cancer Treatment.
Sponsered By Indian Councial Of Medical Research, Delhi.
Organised By Kamla Nehru College Of Pharmacy, Butibori, Nagpur-
441108, Maharastra, India.
ON 25 & 26 March 2017
Ingredient Quantity(g)
Neem stem & bark 0.5
Babul leaves 0.5
Gauva leaves 0.5
Kalmi bark 0.5
Ingredient Quantity(g)
Camphor 0.5
Honey 0.5
Calcium carbonate 3.5
Glycerine 2.0
Para hydroxyl benzoic acid 0.3
Sodium lauryl sulfate 0.5
Sodium chloride 0.2
Distilled water q. s.
Colour Appearance Spredability pH
Greenish brown Homogeneous 3.0 7.50
The stability study was indicated that the formulated tooth paste was good stability.
Anti-microbial activity
The formulated herbal toothpaste exhibited fairly good anti-S. aureus activity as compaired to the standard
drug ciprofloxacin. The formulation exhibited an impressive ZOI of 19.7 mm at MIC of 25μg/mL, whereas
ciprofloxacin exhibited 24.5 mm ZOI at MIC of 6.25μg/mL. Therefore it may be concluded that formulated
tooth paste have potential to exhibit anti-microbial activity.
Comparative study: Formulated herbal toothpaste with marketed preparation
Chart 1: Comparison between pH, Spredability & Foamability with marketed preparation
0
2
4
6
8
10
12
Colgate Dabour Red Dant Kanti Lab
Formulation
8.74 8.55 8.3 8.2
3
4
3 3.5
8
12
8
10
pH
Spredability
Foamability
Chart 2: Comparison of anti-microbial activity with marketed formulation
ZOI
RESULTS AND DISCUSSION
13. Formulation and Evaluation of Tooth Gel from Aloe vera leaves extract
https://www.researchgate.net/publication/321730188_Formulation_and_Evaluation_of_T
ooth_Gel_from_Aloe_vera_leaves_extract
14. Formulation and Evaluation of Herbal Toothpaste: Compared with marketed preparation
https://www.researchgate.net/publication/321731107_Formulation_and_Evaluation_of_H
erbal_Toothpaste_Compared_with_marketed_preparation
15. Formulation and Evaluation of Herbal Toothpaste: Compared with marketed preparation
https://www.researchgate.net/publication/321731318_Formulation_and_Evaluation_of_H
erbal_Toothpaste_Compared_with_marketed_preparation
16. Differential spectrophotometric method for estimation and validation of Verapamil in
Tablet dosage form
https://www.researchgate.net/publication/321731238_Differential_spectrophotometric_m
ethod_for_estimation_and_validation_of_Verapamil_in_Tablet_dosage_form
17. NOVEL IPQCL AND FPQC TEST FOR OPTHALMIC PREPARATION AS PER IP, BP
ANIPQC D USP
https://www.researchgate.net/publication/321731200_NOVEL_IPQCL_AND_FPQC_TE
ST_FOR_OPTHALMIC_PREPARATION_AS_PER_IP_BP_ANIPQC_D_USP
18. Nanoparticle of plant extract: A Novel approach for cancer therapy
https://www.researchgate.net/publication/321731633_Nanoparticle_of_plant_extract_A_
Novel_approach_for_cancer_therapy
19. Differential spectrophotometric method for estimation and validation of Verapamil in
Tablet dosage form
https://www.researchgate.net/publication/321731547_Differential_spectrophotometric_m
ethod_for_estimation_and_validation_of_Verapamil_in_Tablet_dosage_form
20. NANOTECHNOLOGYFOR CANCER THERAPY: RECENT DEVELOPMENTS
https://www.researchgate.net/publication/321731657_NANOTECHNOLOGY_FOR_CA
NCER_THERAPY_RECENT_DEVELOPMENTS
21. Bombax ceiba thorn extract mediated synthesis of silver nanoparticles: Evaluation of anti-
Staphylococcus aureus activity
https://www.researchgate.net/publication/321731559_Bombax_ceiba_thorn_extract_medi
ated_synthesis_of_silver_nanoparticles_Evaluation_of_anti-
Staphylococcus_aureus_activity
22. Formulation and Evaluation of Tooth Gel from Aloe vera leaves extract
https://www.researchgate.net/publication/321731503_Formulation_and_Evaluation_of_T
ooth_Gel_from_Aloe_vera_leaves_extract
Distilled water q. s.
Sr. No. Parameters Observations
1 Colour Greenish brown
2 Odour Characteristic
3 Taste Characteristic
4 Smoothness smooth
5 Relative density 10.2
Sr. No. Parameters Observations
1 pH 8.2
2 Homogeneity Good
3 Abrasiveness Good abrasive
4 Foamability 10 (Good)
5 Moisture content 15.6%
6 Extrudability 90.37
7 Spreadability 3.5cm/sec (Good)
8 stability stable
Extrudability
Extrudability Mean of tree tube
Net wt of formulation in tube (g) 13.5
Wt of toothpaste extruded (g) 12.2
Extrudability amount percentage 90.37
Stability
At 25ºC± 2ºC / 60% ± 5% RH (3rd month):
Colour Appearance Spredability pH
Greenish brown Homogeneous 3.5 8.2
At 30ºC± 2ºC / 65% ± 5% RH (3rd month):
Colour Appearance Spredability pH
Greenish brown homogeneous 3.45 7.95
At 40ºC± 2ºC / 75% ± 5% RH (3rd month):
ZOI
15
20
25
Colgate
Dabour Red
Dant kanti
Lab
Formulation
20.5 21.4 22.5
19.7
ZOI
ZOI
% Moisture Content Camparison
Preparation % Moisture content
Colgate 15.20%
Dabour Red 26.40%
Dant Kanti 8.80%
Lab Formulation 15.60%
The above chart 1,2 and Table shows that formulated herbal toothpaste is having and equal
or near about and engrossing passion over the marketed preparation (Colgate, Dabour Red
and Dant kanti).
CONCLUSION
The research concluded that Herbal toothpaste an emphasizing and more acceptable in
dental research and they are safer with minimum side effect than synthetic preparation.The
formulated toothpaste capable to the tooth and oral hygine and show the anti-microbial
activity against pathogen. The formulation compared with market preparation. Therefore it
shows the equal patronizing and engrossing passion over the marketed formulations
(Colgate, Dabour Red, Dantakanti). The formulated herbal toothpaste has been good scope
in future in nature remedies research and Dental health of public.