The document describes the process of homogenization and differential centrifugation. Tissue is cut up and placed in buffer solution before being ground into small fragments using a homogenizer. The cell fragments are then filtered and placed in a centrifuge to separate out different cell components.

1. LE 6-5A
Homogenization
Homogenate
Tissue
cells
Differential centrifugation

2. • Tissue is cut into pieces and placed in cold isotonic
buffer solution.
• Pieces of tissue are ground into very small fragments
in a type of liquidiser called as homogeniser.
• The suspension of cell fragments is filtered to remove
large pieces of tissue debris
• The filterate is then placed in a centrifuge