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Recycling of Nitrogen




                        ALBIO9700/2006JK
• Nitrogen present in proteins and nucleic
  acids

• Organisms cannot use nitrogen gas in the
  air (N≡N)

• Must be converted from N2 into more
  reactive form (e.g. NH3 or NO3-) – nitrogen
  fixation

                                         ALBIO9700/2006JK
Nitrogen fixation
• Fixation by living organisms
  – Prokaryotes (Rhizobium) - mutualism
  – Lives freely in soil and roots of plants (e.g.
    leguminous plants).
  – Plant roots produce protein (lectins) which bind to
    polysaccharides on cell surface. Bacteria invade roots
    and stimulate cells in the root to divide and develop
    into small lumps or nodules, inside which the bacteria
    form colonies.
  – Bacteria fix nitrogen with nitrogenase
  – Nitrogen gas (N2) to ammonium ions (NH4+)
     •   Supply of H (comes from reduced NADP)
     •   Supply of ATP (metabolism of sucrose)
     •   Anaerobic conditions (maintained through production of
         leghaemoglobin – gets rid of oxygen which diffuses into
         nodules)

                                                               ALBIO9700/2006JK
Rhizobium




            ALBIO9700/2006JK
• Fixation in the
  atmosphere
  – Lightning can cause
    nitrogen molecules to
    react with oxygen to form
    nitrogen oxides


  – Dissolves in rain and
    carried to ground



                                ALBIO9700/2006JK
• Fixation by the Haber
  process
  – Nitrogen and hydrogen
    gases are reacted
    together to produce
    ammonia

  – Ammonia often
    converted to ammonium
    nitrate – inorganic
    fertiliser

                            ALBIO9700/2006JK
• Use of fixed nitrogen by plants
  – In legumes:
     • to make amino acids
     • transported into xylem, distributed to all
       parts and used within cells to synthesise
       proteins
  – Other plants:
     • Rely on fixed nitrogen in soil
     • Root hairs take up nitrate ions by active
       transport
     • Nitrate converted in roots to nitrite (NO2-)
       then ammonia then amino acids which are
       transported to other parts in xylem
                                               ALBIO9700/2006JK
• Assimilation of nitrogen by animals
  – Nitrogen supply comes from proteins in
    diet and nucleic acids
  – Proteins broken down to amino acids
    (digestion), absorbed into blood and
    distributed to cells, then built up again
    into proteins
  – Excess amino acids are deaminated
    in liver, where nitrogen becomes part of
    urea molecules and excreted in urine

                                         ALBIO9700/2006JK
• Return of nitrate to the soil from living
  organisms
  – Protein in cells broken down to amino acids upon
    death (decomposers – protease enzymes)
  – Decomposers use some amino acids, some are
    broken down and nitrogen released as ammonia
  – Ammonia also produced from urea
    (ammonification )
  – Ammonia in soil is converted to nitrite ions (NO2-) and
    then nitrate ions (NO3-) by nitrifying bacteria
    (Nitrosomonas and Nitrobacter)
  – Only occurs freely in well-aerated soils
  – Some plants adapt by supplementing their nitrogen
    intake using animal protein


                                                      ALBIO9700/2006JK
ALBIO9700/2006JK
• Denitrification
  – Denitrifying bacteria provide themselves with
    energy by reversing nitrogen fixation and
    converting nitrate to nitrogen gas, which is
    returned to the air


  – Common in sewage treatment plants, compost
    heaps and wet soils



                                                    ALBIO9700/2006JK

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03 Recycling of Nitrogen

  • 1. Recycling of Nitrogen ALBIO9700/2006JK
  • 2. • Nitrogen present in proteins and nucleic acids • Organisms cannot use nitrogen gas in the air (N≡N) • Must be converted from N2 into more reactive form (e.g. NH3 or NO3-) – nitrogen fixation ALBIO9700/2006JK
  • 3. Nitrogen fixation • Fixation by living organisms – Prokaryotes (Rhizobium) - mutualism – Lives freely in soil and roots of plants (e.g. leguminous plants). – Plant roots produce protein (lectins) which bind to polysaccharides on cell surface. Bacteria invade roots and stimulate cells in the root to divide and develop into small lumps or nodules, inside which the bacteria form colonies. – Bacteria fix nitrogen with nitrogenase – Nitrogen gas (N2) to ammonium ions (NH4+) • Supply of H (comes from reduced NADP) • Supply of ATP (metabolism of sucrose) • Anaerobic conditions (maintained through production of leghaemoglobin – gets rid of oxygen which diffuses into nodules) ALBIO9700/2006JK
  • 4. Rhizobium ALBIO9700/2006JK
  • 5. • Fixation in the atmosphere – Lightning can cause nitrogen molecules to react with oxygen to form nitrogen oxides – Dissolves in rain and carried to ground ALBIO9700/2006JK
  • 6. • Fixation by the Haber process – Nitrogen and hydrogen gases are reacted together to produce ammonia – Ammonia often converted to ammonium nitrate – inorganic fertiliser ALBIO9700/2006JK
  • 7. • Use of fixed nitrogen by plants – In legumes: • to make amino acids • transported into xylem, distributed to all parts and used within cells to synthesise proteins – Other plants: • Rely on fixed nitrogen in soil • Root hairs take up nitrate ions by active transport • Nitrate converted in roots to nitrite (NO2-) then ammonia then amino acids which are transported to other parts in xylem ALBIO9700/2006JK
  • 8. • Assimilation of nitrogen by animals – Nitrogen supply comes from proteins in diet and nucleic acids – Proteins broken down to amino acids (digestion), absorbed into blood and distributed to cells, then built up again into proteins – Excess amino acids are deaminated in liver, where nitrogen becomes part of urea molecules and excreted in urine ALBIO9700/2006JK
  • 9. • Return of nitrate to the soil from living organisms – Protein in cells broken down to amino acids upon death (decomposers – protease enzymes) – Decomposers use some amino acids, some are broken down and nitrogen released as ammonia – Ammonia also produced from urea (ammonification ) – Ammonia in soil is converted to nitrite ions (NO2-) and then nitrate ions (NO3-) by nitrifying bacteria (Nitrosomonas and Nitrobacter) – Only occurs freely in well-aerated soils – Some plants adapt by supplementing their nitrogen intake using animal protein ALBIO9700/2006JK
  • 11. • Denitrification – Denitrifying bacteria provide themselves with energy by reversing nitrogen fixation and converting nitrate to nitrogen gas, which is returned to the air – Common in sewage treatment plants, compost heaps and wet soils ALBIO9700/2006JK