The document summarizes a study on the chemical composition, antioxidant, and antibacterial activity of Thuja orientalis essential oil. Gas chromatography-mass spectrometry analysis identified 19 constituents making up 96.4% of the oil, with the major components being alpha-pinene (83%), sabinene (2.6%), and delta-3-carene (2.5%). The oil showed antibacterial effects against 6 bacterial strains, including both gram-positive and gram-negative, with minimum inhibitory concentration values between 12.8-25.6 mg/ml. It also exhibited antioxidant activity in DPPH free radical scavenging assays, with 49.8% scavenging at 100 μg/ml concentration. Therefore
Chemical Composition and Antifungal Activity of Nigella Sativa L. Oil Seed C...inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Chemical Composition and Antifungal Activity of Nigella Sativa L. Oil Seed C...inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Fatty Acid Pattern and Alkaloids of Echium RauwolfiiEditor IJCATR
The GC/MS analysis of hexane extract revealed the presence of palmitic acid as saturated fatty acid (1.05%), versus oleic acid (2.18%), linoleic acid (1.13%), cis-8,11,14-eicosatrienoic acid (2.12%) as unsaturated fatty acids. On the other hand, CH2Cl2 extract contained palmitic acid methyl ester (3.55%), and methyl isostearate (1.17%) as saturated fatty acids, versus linoleic acid methyl ester (3.57%) and linolenic acid methyl ester (10.01%) as unsaturated fatty acids. The GC/MS analysis of the alkaloid-rich fraction indicated the presence of the pyrazolidine alkaloids petranine (2.97%), 7-angeloyl-9-(2-methylbutyryl) retronecine (4.22%), 7-angeloylretronecine (0.59%) and 9-angeloylretronecine (0.47%).
The butanol extract showed the heights DPPH radical scavenging activity (IC50 = 14.3 µg),. while ethyl acetate extract was very weak in activity (IC50 = 432.3 µg) and no activity with hexane and methylene chloride extract.
The antimicrobial potentials of E. rauwolfii extracts were examined. The inhibition of the fungi species by ethyl acetate extract exert was comparable to Amphotericin B. The inhibition zone of the butanol extract against Streptococcus pneumonia was comparable to Ampicillin, against Pseudomonas aeruginosa was comparable to Gentamicin and Escherichia coli was comparable to Gentamicin.
The cytotoxicity against HePG-2 of ethyl acetate extract and butanol extract were “very strong”, and that of hexane extract and methylene chloride extract were “moderate”, against MCF-7 of ethyl acetate extract and butanol extract were “strong”, that of methylene chloride extract was “moderate”, and that of hexane extract was “weak” and against HCT-116 of butanol extract was “very strong”, of ethyl acetate extract was“strong”, of methylene chloride extract and hexane extract were “moderate”.
Chemical Constituents of Essential Oil and Cytotoxic Activity of Ducrosia asa...Agriculture Journal IJOEAR
— Hydro distillated oil of the aerial parts of Ducrosia assadi Alva. (Umbelliferae) , has been analyzed by GC/MS with two different capillary columns , HP-5MS and HP-Wax .Thirty-four compounds were identified, 94.3% of the total oils. The concentration of citronellol, chrysanthenyl acetate, decanoic acid, decanol and linalool was high in analysis of the oil with both columns. Cytotoxic activity studied on two human cancer cell lines (LS180 and MCF-7) represented moderate cytotoxic activity.
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Gc-Ms Analysis and Antimicrobial Activity of Essential Oil of Senecio Peduncu...IOSR Journals
The chemical composition of the essential oil obtained from the leaves of Senecio pedunculatus collected from the Kumaon region of Uttarakhand, was analyzed by GC-MS. The major constituent was found out to be caryophyllene oxide (23.28%). The antibacterial and antifungal activity of the oil was determined by disc diffusion method. Results showed that the oil exhibited mild antimicrobial activity.
A Comparative study of the Antimicrobial activities of five varieties of esse...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
Phytochemical Screening, Elemental Analysis and Physicochemical Properties of...Premier Publishers
Oil from cassia occidentalis seed was extracted using ethanol, methanol, chloroform and hexane solvents. The hexane solvent gave the highest yield (11.5%) followed by ethanol (10.5%), methanol (9.25%) and chloroform (5.92%). The oil extracted was tested for phytochemicals using test tube method and high-performance liquid chromatography (HPLC). The result indicates the presence of carbohydrate, protein, flavonoids, tannins, alkaloids, saponins and terpenes. The presence of these phytochemicals makes cassia occidentalis seed bioactive. The result of the elemental analysis showed that Na (0.0135±0.0005 mg/ 100 g), Mg (1.9965±0.0005 mg/ 100 g), Mn (0.0540±0.0014 mg/ 100 g), Zn (0.375±0.0042 mg/ 100 g), Cu (0.0265±0.0007 mg/ 100 g) and Cd (0.0035±0.0005mg/100 g) were present in the seed, while Cr and Pb were not detected. The basic physiochemical properties of vegetable oil (iodine, saponification, free fatty acid, acid, peroxide, specific gravity and refractive index) were also analyzed. The results revealed that iodine was 95.02, saponification 83, free fatty acid 12.8 mg/g, acid 25.9 mg/g, peroxide 6.7 mg/g, specific gravity 0.85 and refractive index 1.460 on dry weight bases. Based on this finding, cassia occidentalis seed can be used as an alternative source of vegetable oil.
This research presents a chemical study of essential oil from Aniba duckei Kostermans, known as rosewood, as well as its test against bacteria Aeromonas hydrophila, Bacillus cereus, Serratia sp., and Vibrio alginolyticus. For control, were used pipemidic acid, ampicillin, cephalothin, cefoxitin, chloramphenicol, erythromycin, gentamicin, oxacillin, tetracycline, and vancomycin antibiotics. Oil yield was 1.2% (m/m) with linalool being its major component, with 89.34%. The essential oil was more efficient than all antibiotics tested against Aeromonas hydrophila and Vibrio alginolyticus. Linalool was less efficient than the Aniba duckei Kosterman’s essential oil, but more effective than many antibiotics. The essential oil, when tested against Bacillus cereus, was second only to gentamicin, while linalool presented less effectiveness than both gentamicin and tetracycline against the same bacteria. But both oil and linalool were effective against Serratia. The Aniba duckei Kosterman’s essential oil activity was better than linalool’s in all cases due to the oil’s minor components action and synergy between them, which hinder resistance developed by bacteria. Key-words- Essential oil, Aniba duckei Kostermans, Rosewood, Linalool, Antibacterial
Chemical Composition And Acridicid Properties Of The Moroccan Tanacetum Annuu...inventy
Research Inventy : International Journal of Engineering and Science is published by the group of young academic and industrial researchers with 12 Issues per year. It is an online as well as print version open access journal that provides rapid publication (monthly) of articles in all areas of the subject such as: civil, mechanical, chemical, electronic and computer engineering as well as production and information technology. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published by rapid process within 20 days after acceptance and peer review process takes only 7 days. All articles published in Research Inventy will be peer-reviewed.
Antimicrobial activities of Six Types of Wheat BranIOSRJAC
Six types of wheat bran (Emam, Pohean, Wadielneel, Argeen, Pladi and Debeira) investigated for their biological and antioxidant activities. Physiochemical properties carried for their oil. The polar and nonpolar extracts showed antioxidant activity. These results showed the importance of the wheat bran types as nutritive and medicinal plant. All plant bran types investigated for its biological activity as antifungal and antibacterial. Some types of the plant bran extracts showed antibacterial activity towards Escherichia coli, Pseudomonasaeruginosa, Bacillus subtilis, and Staphylococcus aureus. In addition, some types of the extracts showed antifungal activity towards Candida albicans, and Aspergillusniger. Polar and nonpolar extracts of the plant bran types prepared. The petroleum ether extracts subjected to determination by GC/ MS. It showed different major fatty acids as Linoleic acid followed by Linolelaidic acid and then Palmatic acid. The ethanolic extract of Plaid type separated by column chromatography. The isolated constituents were structurally determined using spectrophotometric analysis as IR, UV, and GC/MS. The ethanolic extract components may be esters and ketones as suggested by GC/MS and their functional groups appeared in the IR readings.
Antimicrobial and antioxidant activities of the essentialChina
Antimicrobial and Antioxidant Activities of the Essential Oils of Some Aromatic Medicinal Plants (Pulicaria inuloides-Asteraceae and Ocimum forskolei-Lamiaceae)
Fatty Acid Pattern and Alkaloids of Echium RauwolfiiEditor IJCATR
The GC/MS analysis of hexane extract revealed the presence of palmitic acid as saturated fatty acid (1.05%), versus oleic
acid (2.18%), linoleic acid (1.13%), cis-8,11,14-eicosatrienoic acid (2.12%) as unsaturated fatty acids. On the other hand, CH2Cl2
extract contained palmitic acid methyl ester (3.55%), and methyl isostearate (1.17%) as saturated fatty acids, versus linoleic acid
methyl ester (3.57%) and linolenic acid methyl ester (10.01%) as unsaturated fatty acids. The GC/MS analysis of the alkaloid-rich
fraction indicated the presence of the pyrazolidine alkaloids petranine (2.97%), 7-angeloyl-9-(2-methylbutyryl) retronecine (4.22%), 7-
angeloylretronecine (0.59%) and 9-angeloylretronecine (0.47%).
The butanol extract showed the heights DPPH radical scavenging activity (IC50 = 14.3 μg),. while ethyl acetate extract was very weak
in activity (IC50 = 432.3 μg) and no activity with hexane and methylene chloride extract.
The antimicrobial potentials of E. rauwolfii extracts were examined. The inhibition of the fungi species by ethyl acetate extract exert
was comparable to Amphotericin B. The inhibition zone of the butanol extract against Streptococcus pneumonia was comparable to
Ampicillin, against Pseudomonas aeruginosa was comparable to Gentamicin and Escherichia coli was comparable to Gentamicin.
The cytotoxicity against HePG-2 of ethyl acetate extract and butanol extract were “very strong”, and that of hexane extract and
methylene chloride extract were “moderate”, against MCF-7 of ethyl acetate extract and butanol extract were “strong”, that of
methylene chloride extract was “moderate”, and that of hexane extract was “weak” and against HCT-116 of butanol extract was “very
strong”, of ethyl acetate extract was“strong”, of methylene chloride extract and hexane extract were “moderate”.
Fatty Acid Pattern and Alkaloids of Echium RauwolfiiEditor IJCATR
The GC/MS analysis of hexane extract revealed the presence of palmitic acid as saturated fatty acid (1.05%), versus oleic acid (2.18%), linoleic acid (1.13%), cis-8,11,14-eicosatrienoic acid (2.12%) as unsaturated fatty acids. On the other hand, CH2Cl2 extract contained palmitic acid methyl ester (3.55%), and methyl isostearate (1.17%) as saturated fatty acids, versus linoleic acid methyl ester (3.57%) and linolenic acid methyl ester (10.01%) as unsaturated fatty acids. The GC/MS analysis of the alkaloid-rich fraction indicated the presence of the pyrazolidine alkaloids petranine (2.97%), 7-angeloyl-9-(2-methylbutyryl) retronecine (4.22%), 7-angeloylretronecine (0.59%) and 9-angeloylretronecine (0.47%).
The butanol extract showed the heights DPPH radical scavenging activity (IC50 = 14.3 µg),. while ethyl acetate extract was very weak in activity (IC50 = 432.3 µg) and no activity with hexane and methylene chloride extract.
The antimicrobial potentials of E. rauwolfii extracts were examined. The inhibition of the fungi species by ethyl acetate extract exert was comparable to Amphotericin B. The inhibition zone of the butanol extract against Streptococcus pneumonia was comparable to Ampicillin, against Pseudomonas aeruginosa was comparable to Gentamicin and Escherichia coli was comparable to Gentamicin.
The cytotoxicity against HePG-2 of ethyl acetate extract and butanol extract were “very strong”, and that of hexane extract and methylene chloride extract were “moderate”, against MCF-7 of ethyl acetate extract and butanol extract were “strong”, that of methylene chloride extract was “moderate”, and that of hexane extract was “weak” and against HCT-116 of butanol extract was “very strong”, of ethyl acetate extract was“strong”, of methylene chloride extract and hexane extract were “moderate”.
Chemical Constituents of Essential Oil and Cytotoxic Activity of Ducrosia asa...Agriculture Journal IJOEAR
— Hydro distillated oil of the aerial parts of Ducrosia assadi Alva. (Umbelliferae) , has been analyzed by GC/MS with two different capillary columns , HP-5MS and HP-Wax .Thirty-four compounds were identified, 94.3% of the total oils. The concentration of citronellol, chrysanthenyl acetate, decanoic acid, decanol and linalool was high in analysis of the oil with both columns. Cytotoxic activity studied on two human cancer cell lines (LS180 and MCF-7) represented moderate cytotoxic activity.
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Gc-Ms Analysis and Antimicrobial Activity of Essential Oil of Senecio Peduncu...IOSR Journals
The chemical composition of the essential oil obtained from the leaves of Senecio pedunculatus collected from the Kumaon region of Uttarakhand, was analyzed by GC-MS. The major constituent was found out to be caryophyllene oxide (23.28%). The antibacterial and antifungal activity of the oil was determined by disc diffusion method. Results showed that the oil exhibited mild antimicrobial activity.
A Comparative study of the Antimicrobial activities of five varieties of esse...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
Phytochemical Screening, Elemental Analysis and Physicochemical Properties of...Premier Publishers
Oil from cassia occidentalis seed was extracted using ethanol, methanol, chloroform and hexane solvents. The hexane solvent gave the highest yield (11.5%) followed by ethanol (10.5%), methanol (9.25%) and chloroform (5.92%). The oil extracted was tested for phytochemicals using test tube method and high-performance liquid chromatography (HPLC). The result indicates the presence of carbohydrate, protein, flavonoids, tannins, alkaloids, saponins and terpenes. The presence of these phytochemicals makes cassia occidentalis seed bioactive. The result of the elemental analysis showed that Na (0.0135±0.0005 mg/ 100 g), Mg (1.9965±0.0005 mg/ 100 g), Mn (0.0540±0.0014 mg/ 100 g), Zn (0.375±0.0042 mg/ 100 g), Cu (0.0265±0.0007 mg/ 100 g) and Cd (0.0035±0.0005mg/100 g) were present in the seed, while Cr and Pb were not detected. The basic physiochemical properties of vegetable oil (iodine, saponification, free fatty acid, acid, peroxide, specific gravity and refractive index) were also analyzed. The results revealed that iodine was 95.02, saponification 83, free fatty acid 12.8 mg/g, acid 25.9 mg/g, peroxide 6.7 mg/g, specific gravity 0.85 and refractive index 1.460 on dry weight bases. Based on this finding, cassia occidentalis seed can be used as an alternative source of vegetable oil.
This research presents a chemical study of essential oil from Aniba duckei Kostermans, known as rosewood, as well as its test against bacteria Aeromonas hydrophila, Bacillus cereus, Serratia sp., and Vibrio alginolyticus. For control, were used pipemidic acid, ampicillin, cephalothin, cefoxitin, chloramphenicol, erythromycin, gentamicin, oxacillin, tetracycline, and vancomycin antibiotics. Oil yield was 1.2% (m/m) with linalool being its major component, with 89.34%. The essential oil was more efficient than all antibiotics tested against Aeromonas hydrophila and Vibrio alginolyticus. Linalool was less efficient than the Aniba duckei Kosterman’s essential oil, but more effective than many antibiotics. The essential oil, when tested against Bacillus cereus, was second only to gentamicin, while linalool presented less effectiveness than both gentamicin and tetracycline against the same bacteria. But both oil and linalool were effective against Serratia. The Aniba duckei Kosterman’s essential oil activity was better than linalool’s in all cases due to the oil’s minor components action and synergy between them, which hinder resistance developed by bacteria. Key-words- Essential oil, Aniba duckei Kostermans, Rosewood, Linalool, Antibacterial
Chemical Composition And Acridicid Properties Of The Moroccan Tanacetum Annuu...inventy
Research Inventy : International Journal of Engineering and Science is published by the group of young academic and industrial researchers with 12 Issues per year. It is an online as well as print version open access journal that provides rapid publication (monthly) of articles in all areas of the subject such as: civil, mechanical, chemical, electronic and computer engineering as well as production and information technology. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published by rapid process within 20 days after acceptance and peer review process takes only 7 days. All articles published in Research Inventy will be peer-reviewed.
Antimicrobial activities of Six Types of Wheat BranIOSRJAC
Six types of wheat bran (Emam, Pohean, Wadielneel, Argeen, Pladi and Debeira) investigated for their biological and antioxidant activities. Physiochemical properties carried for their oil. The polar and nonpolar extracts showed antioxidant activity. These results showed the importance of the wheat bran types as nutritive and medicinal plant. All plant bran types investigated for its biological activity as antifungal and antibacterial. Some types of the plant bran extracts showed antibacterial activity towards Escherichia coli, Pseudomonasaeruginosa, Bacillus subtilis, and Staphylococcus aureus. In addition, some types of the extracts showed antifungal activity towards Candida albicans, and Aspergillusniger. Polar and nonpolar extracts of the plant bran types prepared. The petroleum ether extracts subjected to determination by GC/ MS. It showed different major fatty acids as Linoleic acid followed by Linolelaidic acid and then Palmatic acid. The ethanolic extract of Plaid type separated by column chromatography. The isolated constituents were structurally determined using spectrophotometric analysis as IR, UV, and GC/MS. The ethanolic extract components may be esters and ketones as suggested by GC/MS and their functional groups appeared in the IR readings.
Antimicrobial and antioxidant activities of the essentialChina
Antimicrobial and Antioxidant Activities of the Essential Oils of Some Aromatic Medicinal Plants (Pulicaria inuloides-Asteraceae and Ocimum forskolei-Lamiaceae)
Fatty Acid Pattern and Alkaloids of Echium RauwolfiiEditor IJCATR
The GC/MS analysis of hexane extract revealed the presence of palmitic acid as saturated fatty acid (1.05%), versus oleic
acid (2.18%), linoleic acid (1.13%), cis-8,11,14-eicosatrienoic acid (2.12%) as unsaturated fatty acids. On the other hand, CH2Cl2
extract contained palmitic acid methyl ester (3.55%), and methyl isostearate (1.17%) as saturated fatty acids, versus linoleic acid
methyl ester (3.57%) and linolenic acid methyl ester (10.01%) as unsaturated fatty acids. The GC/MS analysis of the alkaloid-rich
fraction indicated the presence of the pyrazolidine alkaloids petranine (2.97%), 7-angeloyl-9-(2-methylbutyryl) retronecine (4.22%), 7-
angeloylretronecine (0.59%) and 9-angeloylretronecine (0.47%).
The butanol extract showed the heights DPPH radical scavenging activity (IC50 = 14.3 μg),. while ethyl acetate extract was very weak
in activity (IC50 = 432.3 μg) and no activity with hexane and methylene chloride extract.
The antimicrobial potentials of E. rauwolfii extracts were examined. The inhibition of the fungi species by ethyl acetate extract exert
was comparable to Amphotericin B. The inhibition zone of the butanol extract against Streptococcus pneumonia was comparable to
Ampicillin, against Pseudomonas aeruginosa was comparable to Gentamicin and Escherichia coli was comparable to Gentamicin.
The cytotoxicity against HePG-2 of ethyl acetate extract and butanol extract were “very strong”, and that of hexane extract and
methylene chloride extract were “moderate”, against MCF-7 of ethyl acetate extract and butanol extract were “strong”, that of
methylene chloride extract was “moderate”, and that of hexane extract was “weak” and against HCT-116 of butanol extract was “very
strong”, of ethyl acetate extract was“strong”, of methylene chloride extract and hexane extract were “moderate”.
Evaluation of the antimicrobial effect of Thymus capitatus Essential Oil (EO)...IIJSRJournal
Pathogenic bacteria recently turned to be increasingly resistant to the most commonly used antibiotics, thus it becomes an essential need to find another active component that participates in controlling pathogens harms. Thymus capitatus is an endemic aromatic medical plant wildly distributed in the Libyan Green Mountain; therefore, T. capitatus extracted essential oil was used to evaluate the antimicrobial activity. T. capitatus was chemically analyzed to determine the antimicrobial active components using Gas Chromatography-Mass Spectrometric techniques (GC/MS). The plant represented twenty-one chemical compounds including Alpha-thujenes, Gamma-terpinen, Carvacrol, Thymol, Trans-caryophyllene, Aroma dendrene, Alpha-humulene, Ledene, Gamma cadinene, Delta-cadinene, (-)-spathulenol, Caryophullene oxid, Alpha cadinol, Iso aromadrene epoxide, Cis-alpha bisabolene, Vulgarol B, 2-ethyl-4-methyl anisole, Hexanoic acid, oct-3-en-zylester, Phenol l, 2, 3, 5, 6 tetramethyl and Anisole. The antimicrobial activity of T. capitatus aromatic essential oil (EO) was analyzed on several types of pathogens using serial aromatic oil dilutions including (50%, 25%, 13%, 6% and 3%). The results showed 50% oil dilution is the most effective concentration for all tested pathogens including Escherichia coli 7839 ATCC; Klebsiella pneumoniae 700603 ATCC; Staphylococcus aureus 12973 ATCC and Enterococcus faecalis 12697 ATCC with an average diameter of inhibition zone was 28 mm. The same oil concentration (50%) showed antimicrobial effect against pathogenic bacteria isolated from urinary tract infection including S. saprophyticus, E. faecalis, E. coli; and K pneumonia with an average diameter of inhibition zone was 26 mm. Our study may contribute to initial knowledge and would help to discover substances with potential therapeutic uses.
Antibacterial activity of aerial parts of thymus serphyllum linn against clin...IJSIT Editor
In the present research work in vitro antibacterial activity of methanolic extract of aerial parts of
Thymus serphyllum L. growing wild in Kashmir Himalaya was evaluated by agar well diffusion method and
broth dilution assay against nine human pathogenic bacterial strains, known to cause serious infections. The
extract was also screened for the presence of various bioactive phytoconstituents present in the plant. The
extract in the present study possess appreciable potential of inhibiting the growth of all the bacterial strains
at all tested concentrations (30, 60 and 90 mg/ml). The highest sensitivity was exhibited against
Staphylococcus epidermidis MTCC- 435 and Staphylococcus aureus with mean zones of inhibition 20.66 and 20
mm respectively at the concentration of 90 mg/ml. Salmonella typhi showed the least activity with mean zone
of inhibition of 10.00 mm at the concentration of 30 mg/ml. The MIC value ranged between 1.56 to 12.56
mg/ml. The phytochemical analysis of the crude extract revealed the presence of alkaloids, flavonoids,
phenolics, saponins, tannins, cardiac glycosides, terpenes, steroids and carbohydrates. Anthraquinone
glycosides were absent. The present study clearly indicate that the crude methanolic extract of Thymus
serphyllum from high altitude of Kashmir Himalaya (2350 m) shows significant antibacterial activity in
concentration dependent manner.
Formulation and Biological Evaluation of Some Selected Medicinal Plants for A...BRNSS Publication Hub
Objective: The aim of the paper is to assess the anti-inflammatory potential of three medicinal plants using two rat models. Materials and Methods: Soxhlet extraction approaches utilized to separate the constituents of interest. Quantitative analysis has been performed to determine the total phenolic and flavonoid content. Three plants extract employed for the ointment formulation by addition of the extract of Artocarpus heterophyllus (AH), Murraya koenigii (MK), and Punica granatum (PG) in polyethylene glycol (PEG) ointment base, a blend of PEG 600 and PEG 4000, and ratio 7:3, respectively. Two rat models based on chemical induced animals employed for the anti-inflammatory potential. Results and Discussion: All three plants including AH Lam., MK Linn., and PG Linn. extracted for the major component and have shown the gallic acid and quercetin as major component for flavonoid and phenol content. The ointment formulation F3 has showed maximum inhibition (80.95%) at 50 mg/kg dose of carrageenan-induced edema and 83.33% inhibition at 100 mg/kg dose. The ointment formulation F3 has showed maximum inhibition (78.57%) at 50 mg/kg dose of histamine persuade edema and 83.33% inhibition at 100 mg/kg dose. F3 ointment formulation is better than the F2 and F1 formulation in inhibition and in all phases showing its reserve of kinins as well as arachidonic acid. Conclusion: Quantitative and pharmacological evaluation indicated that ointment formulations of AH, MK, and PG have exploit for anti-inflammatory activity. The normal extract has shown the least activity but ointment formulations have shown the better result. The ointment formulations containing plant extracts in 10% amount have better wound healing potential.
INVESTIGATION OF IN-VITRO ANTHELMINTIC AND CYTOTOXIC ACTIVITIES OF ARTABOTRYS...Syed Masudur Rahman Dewan
The methanolic extract of bark of Artabotrys hexapetalus were investigated for in-vitro anthelmintic and cytotoxic activities. Evaluation of cytotoxic activity was done using the brine shrimp lethality bio-assay. The crude methanolic extract showed significant cytotoxic potential (LC50 value of 7.688 μg/ml) comparing with that of standard vincristine (0.839 μg/ml). The other study was undertaken to evaluate anthelmintic activity where albendazole was used as reference standard. Methanolic extract of barks (50 mg/ml) caused paralysis of the worms at 68.33 minutes and death at 84.0 minutes while albendazole (positive control) paralyzed and killed the worms at 17 minutes and 48 minutes respectively at the concentration of 10 mg/ml. The study confirms the significant anthelmintic activities of bark extract of Artabotrys hexapetalus and therefore demands the isolation of active principles through bioassay.
Membrane Stabilizing And Antimicrobial Activities Of Caladium Bicolor And Che...IOSR Journals
The crude methanol extracts of whole plant of Caladium bicolor (Aiton) Vent. and leaf of Chenopodium album L. as well as their pet-ether, carbon tetrachloride, chloroform and aqueous soluble fractions were evaluated for membrane stabilizing and antimicrobial activities. At concentration 1.0 mg/ml, the carbon tetrachloride soluble fraction of C. bicolor inhibited 43.92±1.63% and 38.08±0.83 % hypotonic solution and heat induced haemolysis of RBCs, respectively. Among the extractives of C. album, the aqueous soluble fraction inhibited 47.11±0.49 % and 36.73±0.76 % hypotonic solution and heat induced haemolysis of RBCs as compared to 72.79 % and 42.12 % by acetyl salicylic acid (0.10 mg/ml), respectively. C. bicolor test samples demonstrated zone of inhibition ranging from 6.0 to 20.0 mm. The chloroform soluble fraction showed the highest zone of inhibition (20.0 mm) against Staphylococcus aureus. The test samples of C. album displayed zone of inhibition ranging from 7.0 to 13.0 mm. The highest zone of inhibition (13.0 mm) was showed by the chloroform soluble fraction against Salmonella paratyphi
Essential oil of Baccharis as a sustainable alternative for small farmers in ...Agriculture Journal IJOEAR
Abstract— This work aimed at evaluating the insecticidal and repellent effect of essential oils of Baccharis trimera and Baccharis articulate leaves against the maize weevil in maize grains. The essential oil was extracted by hydrodistillation and the chemical composition showed that the major constituent was the carquejil acetate (73.6%) for B. trimera and β-pinene (22.3%) and β-cariofilene (21.5%) for B. articulate. Our results show that B. trimera oil was insecticidal and repellent to maize weevil. About 100% of mortality was achieved using concentrations of 0.32 μL.cm-2, after 24 h of exposure and the lower concentration 0.065 µL.cm-2 obtained similar results in 96 h. The LD50 value for B. trimera essential oil was 8.4 μL/ Petri dishes or 0.05 µL.cm-2. The results obtained indicate that the effect insecticidal of the essential oil of B. trimera is probably because of the presence of carquejil acetate. B. articulate showed low insecticidal activity. The values of the Preference level varied from -0.6 (0.065 μL.cm-2) to -0.9 (0.65 μL.cm-2) for B. trimera oil and -0.4 (0.065 μL.cm-2) to -0.7 (0.65 μL.cm-2) for B. articulata oil, being lower than -0.10. The minimum limit to consider that a plant has repellency activity, demonstrating that the both plants oils presents repellency activity. Although from an economical point of view synthetic chemicals are still more frequently used as repellents, we find the essential oil of B. trimera have potential to be used sustainably as bioinseticide by the small farmers.
Similar to Chemical composition, Antioxidant and Antibacterial activity of Thuja orientalis (20)
Molecular docking studies of abelmoschus esculentus for anti diabetics and a...Jing Zang
Okra (Abelmoschus esculentus (L) Moench) or bhendi also known as ladies finger is an important vegetable crop in India, and African regions. Abelmoscus esculentus having the medicinal property of anti inflammatory , anti diabetics, anti oxidant activities . In this studies we are going to analysis the anti diabetics and anti inflammatory property of Abelmoscus esculentus by using molecular docking studies. Diabetics is a major cause of death and the number of new cases, as well as the number of individuals living with Diabetics, is expanding continuously. Now a days It is one of the most common diseases in the worldwide .Foot ulceration remains a major health problem for diabetic patients and has a major impact on the cost of diabetes treatment. One major complication of diabetes is foot ulceration, which occurs in as many as 15–25% of type 1 and type 2 diabetic patients over their lifetimes. The phytochemicals of Abelmoscus esculentus are analysed and optimized with the Arguslab to investigate the interactions between the target compounds and the amino acid residues of the Mafa and Mmp9. All the compound have shown binding pose between from – 3.25 to -7.95 and -7.95 into -11.40 out of ten compound . [E,E] Farenesal with Mafa protein and gossypol with Mmp9 protein show best ligand energy -10.55 and -8.88 Kcal/mol with 1 and 1 hydrogen bond of distance is 3.0 and 2.3 respectively .
Molecular docking studies of gloriosa superba for anti cancer and anti tuberc...Jing Zang
Gloriosa superba is a medicinal plant generally found in western parts of Tamilnadu and kerala in India. Gloriosa superba having the medicinal property of anticancer, antibacterial, antifungal, anti Tuberculosis and mutagenic activities. In this studies we are going to analysis the anti cancer and anti tuberculosis property of Gloriosa superba by using molecular docking studies. Cancer is a major cause of death and the number of new cases, as well as the number of individuals living with cancer, is expanding continuously. Cervical cancer is one of the most common cancers among women worldwide . Tuberculosis is a common and often deadly infectious disease caused by mycobacteria , usually Mycobacterium tuberculosis in humans. Tuberculosis usually attacks the lungs but can also affect other parts of the body. The phytochemicals of Gloriosa superba are analysed and optimized with the Arguslab to investigate the interactions between the target compounds and the amino acid residues of the E7 and DAHP. All the compound have shown binding pose between from – 3.25 to -7.95 and -7.95 into -11.40 out of ten compound .Chrysophanic acid with E7 protein and Colchicine with DAHP protein show best ligand energy -9.52049 and -7.47679Kcal/mol with 1 and 3 hydrogen bond of distance is 2.3 and 2.2,2.9 and 3.2 respectively .
A systemic review on antibiotic use evaluation in paediatricsJing Zang
Drug utilization is the marketing, distribution, prescription, and use of drug in a society, with special emphasis on the resulting medical, social and economic consequences. Antibiotics are valuable discoveries of modern medicine and their definitive and or appropriate use has led to a decline in the morbidity and mortality associated with various infectious diseaseswhile inappropriate use of antibiotics can increase morbidity, mortality, patient cost and bacterial antibiotic resistance.Antimicrobial agents are among the most commonly prescribed drug in Paediatrics. Because of an overall rise in health care costs, lack of uniformity in drug prescribing and the emergence of antibiotic resistance, monitoring and control of antibiotic use are of growing concern and strict antibiotic policies should be warranted. The caution use for antimicrobial agents is very important as their unavailability or resistance can be life threatening. Irrational drug use is a common practice in developing countries. In India, clinician often prescribe three or four drugs to treat the most trivial conditions for the sake of satisfying the patients need to receive drugs or the drug sellers need for profit. Thus drug use evaluation studies are required for all drugs in general and particularly for antibiotics.
A review on medicinal properties of Camel milkJing Zang
Many research findings proved that Camel milk is closer to human milk than any other milk. It is often easily digested by lactose-intolerant individuals. It is rich in healthy vitamins and minerals, especially B vitamins, vitamin C and iron. The lactoferrin in camel milk has antibacterial, antiviral and anti-tumor properties. It contains disease-fighting immunoglobulins which are small in size, allowing penetration of antigens and boosting the effectiveness of the immune system. It is a rich source of insulin and also it containing approximately 52 units of insulin in each liter of camel milk, making it a great treatment option for Type 1 or Type 2 diabetics as well as Gestational Diabetes. This review focused on the medicinal properties of camel milk which will be more useful to generate value added products formation from camel milk.
Formulation and Evaluation of Solid dispersion for Dissolution Enhancement of...Jing Zang
Nifedipine, a calcium channel blocker antihypertensive drug, is a poorly water soluble drug and belongs to BCS class II. The objective of the research work was to formulate and optimize solid dispersions (SDs) of a poorly water soluble drug, nifedipine, with sodium starch glycollate, croscarmellose sodium, eudragit E-100. Solid dispersions were prepared by solvent evaporation techniques in different weight ratios of polymers. The results indicated that homogeneous or heterogeneous conditions during the preparation methods employed governed the internal structures of the polymer matrices while retaining the drug in an amorphous form. The physical mixtures and solid dispersions were subjected to drug content and dissolution test. The best formulation, nifedipine with croscarmellose sodium in 1:7 ratio, among all was further adsorbed on neusilin US2 to form ternary mixture. The increased dissolution was achieved by more than 70percent and 30percent comparatively to the nifedipine API and marketed product respectively. The tablet dosage form prepared from ternary mixture was stable at stressed conditions 40±2°C and 75±5% RH. The release kinetics of drug from formulation and marketed product follows peppas model. The similar factor f2 was within limit for the product at stressed conditions with the product at room temperature at the same time.
Glucose lowering potential of hydromethanolic extract of RauwolfiaJing Zang
The objective of the present study was to evaluate the phytochemistry, acute toxicity and glucose lowering potential of hydromethanolic roots extract (HMREt) of Rauwolfia serpentina. The qualitative analysis of HMREt showed the presence of many important phyto-constituents except anthraquinones, carbohydrates and saponins whereas quantitatively it found rich in total phenols. In acute toxicity study, orally administrated HMREt from 5-250 mg/ kg was observed safe and non-sedative while its doses from 500-2500 mg/kg were found sedative and induced mortalities (17-100%) within 4 hours of administration. The median lethal dose (LD50) of same extract was calculated as 1412.54 mg / kg (log LD50 = 3.15 mg/ kg) from log doses verses probit graph. The HMREt in doses of 50, 100 and 150 mg/kg induced significant percent decrease in blood glucose level at 30, 60 and 120 minutes in normo-hyperglycemic test mice as compared to control and negative control groups (p<0.05). The results concluded that HMREt has glucose lowering potential either by developing glucose tolerance or by pancreatic action in normo-hyperglycemic mice.
Nutritive and Anti-nutritive composition of Wild grown Canavalia gladiata seedsJing Zang
The wild Canavalia gladiata seeds were widely distributed in Nupeland, North Central Nigeria. It was obtained and processed by decoating, sun drying and grinding into powder. Using petroleum ether (40-60oC), the fats was extracted, the protein content, ash content, crude fibre, moisture, carbohydrate with respective values of 3.60±0.14, 11.1±0.83, 4.25±0.11, 3.39±0.27, 5.85±0.47 and 72.3±0.08 % as well as the mineral contents were determined using standard methods. The mineral composition determined from the C. gladiata seeds shows higher values of potassium, zinc, iron and calcium 25.15±0.03, 25.89±0.27, 18.3±0.14 and 17.25±0.49 mg/100 g respectively. This seed analyzed contains low yield of anti-nutritional contents which suggested that, it could be safe for human consumption since it fell below the lethal dose limit. The sample contains reasonable amount of essential and non-essential amino acids with yield varying between 48 and 52%. The presence of unsaturated and saturated fatty acids in the C. gladiata was 96 and 4% respectively. The higher percentage of unsaturated fatty acid present makes this seed desirable for consumption by the person with heart diseases. In addition, from the data obtained this oil becomes attractive options for commercial purposes since it is suitable for the manufacture of soaps, lubricating oil, candles as well as pharmaceutical industries.
Pharmacovigilance in South Africa: Undocumented undergraduate training and pr...Jing Zang
Pharmacovigilance is a clinical discipline that is gaining more and more attention worldwide and in Africa. The rolling out of large scale programs on HIV/AIDS, tuberculosis and malaria has heightened the need to step up efforts to have pharmacovigilance concepts to be operationalized in clinical practice. A quick search in PubMed and Google Scholar and a review of available literature was conducted in order to establish whether medical, nursing and pharmacy undergraduate students are taught pharmacovigilance concepts and skills for effective practice. It seems that there is a paucity of data on the undergraduate training in pharmacovigilance in South Africa. It may be that there might be inadequate training on pharmacovigilance during undergraduate training of medical, nursing and pharmacy students in South Africa. More studies are needed to document the views and experiences of South African students and healthcare professionals on training and practice of pharmacovigilance.
Black Seed (Nigella sativa) Possess Bioactive Compounds Act as Anti-Helicobac...Jing Zang
ABSTRACT
Gastrointestinal associated problems are physiological processes effects almost every individual at some stage of their life. Among the various plants studied previously Nigella sativa possess numerous therapeutic properties including its anti-ulcer potential. This seed carries significant anti-ulcer properties arbitrated by antimicrobial activities specifically against gastric damage induced by Helicobacter pylori. Evidence is available supporting the utilization of NS and its bioactive components in a daily diet for health improvement. This review is envisioned to emphasis on the curative role of NS and to provide an evidence for being a functional food to protect from a range of malaises. An attempt is also made to emphasize aspects that need further investigations for it to be use in clinics in future.
In vitro enzyme inhibition studies on new sulfonamide derivatives of 4-tosyl ...Jing Zang
Sulfonamides are considered to be pharmaceutically important class of compounds. In the present work, N-(2,4-dimethylphenyl)-4-toluenesulfonamide (3) was synthesized by the reaction of 2,4-dimethylaniline (1) and 4-tosyl chloride (2; 4-methylbenzenesulfonyl chloride) using 10% aqueous Na2CO3 solution as reaction medium. At the second step, the synthesized molecule 3 was made to react with different alkyl/aralkyl halides (4a-o) to yield the target compounds, 5a-o, using N,N-dimethylformamide (DMF) as reaction medium and lithium hydride as an activator. The synthesis of all the compounds was verified by spectral techniques using IR, 1H-NMR and EIMS; and further examined for their anti-enzymatic activities. The synthesized compound 5f represented a suitable inhibitory potential against α-glucosidase and lipoxygenase enzymes.
In vitro sun screening activity of Sri Lankan orthodox black tea (Camellia Si...Jing Zang
Currently, there is demand for the development of herbal sunscreen formulations to suppress harmful effects of UV rays. In this regard, this study, was conducted to investigate the sun screen potential of Sri Lankan Orthodox black tea (made from buds and top most leaves of Camellia sinensis L plant) using three grades (Dust No:1, Broken Orange Pekoe and Orange Pekoe) using UV spectroscopic technique and Mansur equation. Sun Protection Factor (SPF) value was determined using 20% aqueous extracts (Black tea brews). The results revealed that all three tea samples had markedly high absorbance values (1.4 to 4.2) at 290-320 nm range and SPF values above 15 which are considered as the threshold value for good sunscreen. The SPF value of Dust No:1, B.O.P.F and O.P were respectively 36, 23 and 22. This is a novel finding for Sri Lankan black tea. It is concluded that Sri Lankan black tea, especially, Dust No: 1 can function as an efficient sunscreen agent and has great promise to be developed as cheap, safe and effective topical botanical sunscreen acting via multiple mechanisms (considering its other reported bioactivities).
HPTLC determination of carotenoid profile in the leaf and bark samples of lor...Jing Zang
Influence of host plants on the carotenoid profile of Loranthus longiflorus leaf and bark samples collected from Casuarina equisetifolia and Ficus religiosa host trees were determined by HPTLC method. The methanol extract of L. longiflorus leaf samples obtained from C. equisetifolia host trees showed 9 compounds while it was 8 compounds in the leaf samples collected from F. religiosa host tree. Among the compounds, 5 and 3 compound in each sample, respectively, was identified as carotinoids while the others were unknown. Four compounds from each leaf samples collected from C. equisetifolia (peak no. 4- 6 & 8) and F. religiosa (peak no. 1-3 & 6) host trees showed similar Rf values (0.15, 0.19, 0.23 & 0.53, respectively). Similarly, the methanol extract of L. longiflorus bark sample collected from C. equisetifolia and F. religiosa host trees contained 8 compounds each. Of these compounds only 3 from each sample was identified as carotenoids whereas others were unknown and none of these compounds showed any similar Rf values. One compound from leaf and park samples of L. longiflorus collected from C. equisetifolia (peak no. 6 & 4) and F. religiosa (peak no. 4 & 3) showed similar Rf values (0.23 & 0.26), respectively.
Antinociceptive and Diuretic Activities of Tagetes erecta LinnJing Zang
In the present investigation, the possible antinociceptive and diuretic activities of methanolic extract of Tagetes erecta has been tested in animal models. The methanol extract of both aerial part and root of the plant exhibited significant antinociceptive activity at higher dose (400 mg/kg body weight) in Swiss albino mice. The root extract was found to reduce the writhing more effectively than that of aerial part which is comparable to that produced by aminopyrine, used as standard drug. In addition, crude whole plant extract was also showed efficient diuresis at higher dose 400 mg/kg tested. Diuretic activity was proved by the electrolyte loss ratio (Na+/K+ excretion ratio) and we used furosemide as the reference.
PREVAILENCE OF MIGRIANE IN A LOW INCOME COMMUNITY OF KARACHIJing Zang
Migraine is the most common problem affecting large population, with prevalence frequency 10-12 %. This study was conducted to evaluate the prevalence of migraine in a low income community in Karachi from June-Oct, 2013. Three hundred and seven participants were involved in this study. For this purpose cross-sectional community based questionnaire was designed in accordance with the diagnostic criteria given by International Headache Society. Data collection was carried out by personal visit to patients and through clinics. It was observed that females (65.5%) are more affected from migraine then male. 32.9% housewives reported that they are suffering from migraine. This medical problem is common among youngsters (38.1%) than old citizens. Employees working in different organizations (39.7%), were found to be mostly affected from migraine then self employed persons. Majority of the migraine patients (41%) reported that they are suffering from common symptoms including: photophobia, phonophobia, nausea, vomiting etc. Dietary habits of individuals were found to be closely associated with migraine such as use of caffeine, chocolate are prominent; and their use is common among 75% patients cumulatively. It was evaluated that certain disease conditions i.e. stress (33.6%), depression (22.1%) and anxiety (18.9%) are more common among sufferers of migraine.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
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2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
2. Wajaht et al., World J Pharm Sci 2014; 2(1): 56-61
anhydrous sodium sulphate and kept in glass vials
at – 4oc prior to analysis.
The oil was dissolved in dimethyl sulphoxide and
added to the medium, and then diluted in order to
obtain concentrations in the range of 0.25– 25.6
mg/ml. Inoculum suspension with a final
concentration of 106 cfu/ml was added to plate. The
MIC was defined as the lowest concentration of the
essential oil at which the microorganism does not
demonstrate any visible growth after incubation at
37 ⁰C for 24 h.
Chemical composition:
GC–MS analysis: GC–MS analysis was carried on
a Varian Gas Chromatograph series 3800 fitted
with a VF-5 ms fused silica capillary column (60 m
× 0.25 mm, film thickness 0.25 µm) coupled with a
4000 series mass detector under the following
conditions: injection volume 0.5 µl with split ratio
1:60, helium as carrier gas at 1.0 ml/min constant
flow mode, injector temperature 230 ⁰C, oven
temperature was programmed from 60 to 280 ⁰C at
3 ⁰C/min. Mass spectra: electron impact (EI+)
mode, 70 eV and ion source temperature 250 ⁰C.
Mass spectra were recorded over 50–500 a.m.u
range.
Antioxidant activity
DPPH free radical-scavenging activity: DPPH free
radical scavenging activity was evaluated by
measuring the scavenging activity of the essential
oil on stable 2,2-diphenyl- 1-picryl hydrazyl
radical. A 0.5 mM solution of DPPH in methanol
was prepared and a stock solution of oil sample (1
mg/ml) in methanol was prepared. Various
concentrations (20–100 µg/ml) were added to 1ml
(0.5 mM DPPH) and final volume was made to 3
ml with methanol. The mixture was shaken
thoroughly and kept standing at room temperature
for 10 min. Then, the absorbance of the mixture
was measured at 517 nm on a spectrophotometer. A
decrease in the absorbance indicates an increase in
DPPH-radical scavenging activity.
Antimicrobial assay
Microbial strains and culture media: The
antibacterial activity of the essential oil of Thuja
orientalis were tested against a panel of six
bacterial strains obtained from Microbial Type
Culture Collection (MTCC), Institute of Microbial
Technology, Chandigarh, India. The bacterial
strains used were Bacillus subtilis (MTCC-441), P.
aeruginosa (MTCC-1688), S. aureus (MTCC 96),
K. pneumonia (MTCC-19), E. coli (MTCC-443)
and P. vulgaris (MTCC-1771). Bacterial strains
were grown on nutrient agar plates at 37 ⁰C and
maintained on nutrient agar slants. Cell suspension
of microorganisms in 0.9% NaCl was adjusted at
0.5 McFarland to obtain approximately 106 cfu/ml.
Antimicrobial activity and determination of
minimum inhibitory concentration (MIC): The
antibacterial susceptibility tests were carried out
using the agar well diffusion assay/microdilution
assay with some modification. First Muller Hinton
medium was prepared and 0.5% of tween-20 was
dissolved per 100 ml of agar medium in order to
facilitate proper diffusion of agar in the oil. 20 ml
aliquot was transferred in to each boiling tube.
After this sterilization of boiling tubes was carried
out in autoclave. Temperature of tubes was
regulated upto 38⁰C and oil samples were added in
the concentration range of 0.2-25.6 mg/ml.
The percentage inhibition was calculated by the
following equation:
DPPH radical scavenging= [(Ac- As)/Ac)×100]
Where, Ac is the absorbance of the control and As
is the absorbance of the sample.
L-ascorbic acid served as positive control.
RESULTS AND DISCUSSIONS
The chemical composition of the essential oil
isolated from the fruits of thuja orientalis analysed
by gas chromatography-mass spectrometry are
presented in the table 1, given below. The analysis
revealed the presence of nineteen constituents
representing 96.4% of the total oil. The order of
elution of various constituents from RTx-5
cpolumn are shown in figure1. The major
constituents of the oil were alpha-pinene (83%),
sabinene (2.6%), delta-3-carene (2.5%). The
percentage yield of oil was found to be .13 %
(v/w), as per their fresh weight. The components of
oil were classified into monoterpenes, accounting
94.7% and sesquiterpene hydrocarbons accounting
0.8%. Monoterpenes are represented by oxygenated
monoterpenes 0.4% while as oxygenated
sesquiterpenes are weakly represented by 0.5%.
The contents of the tube were transferred into
plates which were kept under laminar flow and
allowed to dry for 30 minutes. Finally bacteria
were inoculated from fresh cultures into the broth
and its turbidity was adjusted in the range of .080.13 at 625nm. Later 3µl of inoculums of each
bacteria was added into the plates. Streptomycin
sulphate (1000mg/l) was used as positive control
for bacteria. The MIC of oil was determined by the
microdilution method, recommended by the
National Committee for Clinical Laboratory
Standards (NCCLS) as described previously [16]
The in vitro antimicrobial activity of essential oil
was qualitatively and quantitatively assessed by the
presence or absence of inhibition zones, zone
diameters and minimum inhibitory concentration
(MIC) values. Results from antimicrobial activity
57
3. Wajaht et al., World J Pharm Sci 2014; 2(1): 56-61
by agar well diffusion method are presented in
table 2. The essential oil of Thuja orientalis
showed significant antibacterial effect against the
entire test microorganisms used for screening. This
oil was mainly effective against P. vulgaris and
E.coli with highest inhibition zones of 24 and 22
mm respectively. Streptomycin sulphate was used
as a positive control which showed inhibition zones
between 20 -30 mm against different
microorganisms tested. Therefore the antibacterial
activity of Thuja orientalis essential oil seems
closer to reference antibiotic. The MIC value of all
tested bacteria was found between 12.8-25.6
mg/ml. As can be clearly seen from the
photographs (Figure 2) that growth of all the six
bacteria was observed on the plates between the
concentration range of 0.4-12.6 mg/ml. While as no
growth of bacteria was observed on the plate at a
concentration of 25.6 mg/ml of oil. The previous
reports on antimicrobial activity of Thuja orientalis
essential oil against different microorganisms
support our result [17]. The antimicrobial activity
of this oil against K. pneumonaie, P. Aeroginosa
and P. Vulgaris is reported first time.
act as donor for hydrogen atoms in the
transformation of DPPH radical into its reduced
form DPPH2 was investigated. The examined oil
was able to reduce the stable purple coloured
DPPH radical into yellow coloured DPPH2. The
aforementioned oil showed promising radical
scavenging activity at concentration of 100μl/ml.
The results are plotted in the form of graph
(Figure.3). This plant oil exhibited prominent
DPPH free radical scavenging activity of 49.8% in
comparison to ascorbic acid and α-tocopherol
standard which showed the activity of 67.95 and
71.2%, respectively. The DPPH radical scavenging
assay is commonly employed in evaluating the
ability of antioxidants to scavenge free radicals.
This method has been used extensively to predict
the antioxidant activity because of the relatively
short time required for analysis. The change in
absorbance at 517 nm is used as a measure of the
scavenging effect of a particular sample for DPPH
radicals. The more rapidly the absorbance
decreases, the more potent the antioxidant activity
of the sample in terms of its hydrogen atomdonating capacity. It should be noted that our study
showed major constituent α-Pinene 83.0% instead
of 52% reported in literature. Antioxidant potential
is also first time reports from the essential oil of
Thuja Orientalis.
DPPH(1,1-diphenyl-2-picryl hydrazyl free radical)
free radical scavenging capacity of the essential oil
was measured by DPPH assay under in-vitro
conditions. The ability of the examined extract to
Table1: Chemical composition of the essential oil of Thuja Orientalis
S. No
Compound
% Peak Area
Methods of identification
1
α-Pinene
83.0
MS, RI, Std
Camphene
0.5
MS, RI
2
MS, RI
3
β-Phellandrene
1.0
MS, RI, Std
4
Tricyclene
2.2
Sabinene
2.6
MsS, RI
5
α-Phellandrene
0.2
MS, RI
6
MS, RI, Std
7
δ-3-Carene
2.5
MS, RI
8
α-Terpinene
Tr
P-Cymene
Tr
MS, RI
9
Limonene
1.1
MS, RI
10
1,8-cineole
0.2
MS, RI
11
γ-Terpinene
0.1
MS, RI
12
α-Terpinolene
1.5
MS, RI
13
4-Terpineol
0.2
MS, RI
14
β-Fenchol
Tr
MS, RI
15
β-Caryophyllene
0.3
MS, RI
16
α-Caryophyllene
0.4
MS, RI
17
Germacrene D
0.1
MS, RI
18
Cedrol
0.5
19
Total (%)
96.4
Monoterpene hydrocarbons
94.7
Oxygenated monoterpenes
0.4
Sesquiterpene hydrocarbons
0.8
Oxygenated sesquiterpenes
0.5
58
4. Wajaht et al., World J Pharm Sci 2014; 2(1): 56-61
Table 2: In Vitro Anti Microbial Activity of Thuja Orientalis Essential Oil and reference antibiotic determined
with Agar well Diffusion Method
S.No. Test bacteria
Zones of
Zone
of MIC (in mg/ml)
inhibition
inhibition of
(in mm)
antibiotic (in
mm)
Gram-Positive Bacteria
1
S. Aureus MTCC 96
2
B.subtilis MTCC 441
3
K.pneumoniae MTCC 19
Gram-Negative Bacteria
4
E.coli MTCC 443
5
P.aeroginosa MTCC 1688
6
P.vulgaris MTCC 426
FIG: 1
20
15
16
18
30
17
12.8-25.6
12.8-25.6
12.8-25.6
22
19
24
20
30
20
12.8-25.6
12.8-25.6
12.8-25.6
Order of elution of various constituents of analysed essential oil
Major components:
1= α-Pinene
2= β-Phellandrene
3= Tricyclene
4= Sabinene
59
5. Wajaht et al., World J Pharm Sci 2014; 2(1): 56-61
Fig 2 Plates shows antimicrobial activity of Essential oil.
Fig 3 Antioxident potential of essential oil from Thuja Orientalis
MP2; Essential oil, AA; Ascorbic acid, conc; concentration (µg/ml).
60
6. Wajaht et al., World J Pharm Sci 2014; 2(1): 56-61
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