LEIDY JOHANA HINCAPIÉ RESTREPO MEDICINE STUDENT Function of the recently discovered enhancer RNA molecules may open new avenues for gene therapy and Powerful technology of single cell RNA sequencing to track the genetic development of a human and a mouse Embryo. 1. presentation 2. Introduction, overview 3. Function of the recently discovered RNA molecules enhancer May open new avenues for gene therapy 4. Powerful technology of single cell RNA genetic sequencing to track the development of a human and a mouse Embryo 5. Utility for medicine 6. bibliography

1. Function of the recently discovered enhancer RNA
molecules may open new avenues for gene therapy
and
Powerful technology of single cell RNA sequencing
to track the genetic development of a human and a
mouse Embryo
Molecular Biology
LEIDY JOHANA HINCAPIÉ RESTREPO
MEDICINE STUDENT
3RD SEMESTER

2. FOLDING

3. FOLDING

4. INTRODUCTION
• Regulated:
• Promoters located in the beginning of
genes.
• Enhancers which may be located as
far as thousands of base pairs away
from the gene they regulate.
Gene
transcription
• RNA sequence.
Diagnostic
technique

5. Function of the recently discovered enhancer RNA
molecules may open new avenues for gene therapy

6. Function of the recently discovered enhancer RNA
molecules may open new avenues for gene therapy
GENE TRANSCRIPTION
PROMOTER DNA
sequence where the RNA
polymerase binds.
1. Initiation.
2. Elongation.
3. Termination.

7. Function of the recently discovered enhancer RNA
molecules may open new avenues for gene therapy
• Promoters located
in the beginning of
genes.
• Enhancers which
may be located as
far as thousands of
base pairs away
from the gene they
regulate.
• A new enhancer in
macrophages.
Regulated

8. • The activation from
transcription factor
binding to histone
acetylation.
• eRNA transcription.
Function of the recently discovered enhancer RNA
molecules may open new avenues for gene therapy
H3K4me1/2
long-term epigenetic changes in cells.
Information about the function of this new type of RNA.

9. OBSERVATION
I think it is extremely important
to advance new forms of
treatment for genetic diseases
and other damage to cellular
RNAs sequences.

10. Powerful technology of single-cell RNA sequencing to
track the genetic development of a human and a mouse
Embryo

11. Powerful technology of single-cell RNA sequencing to
track the genetic development of a human and a mouse
Embryo
Single cell RNA
sequencing
Determine the precise
nature of the total
gene transcripts.
Determine all of the
genes that are actively
expressed in a cell.

12. • It is a much more
comprehensive analysis
than was achievable
before.
• The technique requires a
very minimal amount of
sample material just one
cell.
Powerful technology of single-cell RNA sequencing to
track the genetic development of a human and a mouse
Embryo

13. • Genetic diagnosis BRCA1 y BRCA2.
• Reproductive medicine.
Powerful technology of single-cell RNA sequencing to
track the genetic development of a human and a mouse
Embryo

14. OBSERVATION
Gives us a better notion of
how the expression is Given
genica since the beginning of
the cell division and how you
can help in the diagnosis of
genetic mutations.

15. MEDICAL UTILITY

16. • A minimum amount of sample material.
• More complete analysis.
• Timely diagnosis.
MEDICAL UTILITY

17. • Identifying genetic damage to improve the
quality of life of patients and in the case of
breast cancer.
MEDICAL UTILITY

18. • University of California - Los Angeles (2013, July 31).
Scientists analyze genetic makeup of human and mouse
embryos in amazing detail. (On Line). ScienceDaily.
• University of Eastern Finland (2013, August 13).
Enhancer RNAs may open new avenues for gene
therapy. (On Line). ScienceDaily. Retrieved August 24.
• MARTINEZ SÁNCHEZ, Lina María. Biología molecular.
7 ed. Medellín: UPB. Fac. de Medicina, 2012. 85 - 91 p.
BIBLIOGRAPHY