Introduction to medical mycology, basic concepts about superficial and deep mycoses taxonomy , classification & general characteristics of Various medically important fungi, Names of fungi & diseases caused by them; superficial mycoses, candida, dermatophytes, opportunistic fungi, subcutaneous mycoses.
Call Girl Bangalore Nandini 7001305949 Independent Escort Service Bangalore
Medical Mycology notes
1. Module- 1
Topic name
Introduction to medical mycology
Sub code: BMLT5001
Sub Name: Virology and mycology
Department: Department of MLT, SMAS
Faculty: A. Vamsi Kumar
Designation : Assistant professor
Sem- V
2. SYLLABUS OVERVIEW
Unit-1 Mycology 8 hours
Introduction to medical mycology, basic concepts about superficial and deep mycoses taxonomy , classification &
general characteristics of Various medically important fungi.
Unit-2 Fungal infection 8 hours
Names of fungi & diseases caused by them; superficial mycoses, candida, dermatophytes, opportunistic fungi,
subcutaneous mycoses
Unit-3 Virology 7 hours
Introduction, classification of virus, collection, Transport, & Storage of sample for Viral diagnosis. Staining Techniques
used in virology,
Processing of sample for viral diagnosis (Egg Inoculation & Tissue culture)
Unit-4 RNA Viruses 9 hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies, Arbo viruses, Measles Mumps, Rubella, HIV
Unit-5 DNA Viruses 8 hours
DNA viruses: Small Pox, HSV, CMV, EBV, Varicella Zoster, Hepatitis, Adeno virus
Unit - 6 Next generation PCR and it’s modren applications 8 Hours
PCR and Next genration DNA Secquencing techniques to identify viral genome: History of PCR, Priciple of PCR, Stages
of PCR, procedure of PCR, Concept of optimization of PCR, Applications of PCR, Advancements in PCR technology, DNA
sequecing techniques, Methods of DNA Sequencing, Next generation DNA sequencing techniques
3. Course outcomes
On completion of this course, the student can be able to:
• Perform basic diagnostic tests to identify fungus
4. Learning outcomes
1 Understand the world of microbes, fungi and lichens
2 Appreciate the adaptive strategies of the microbes, fungi and lichens
3 Understand the economic and pathological importance of bacteria and fungi
49. Introduction
Sabouraud Dextrose Agar
(SDA)
• Sabouraud Dextrose Agar (SDA) is used for the
isolation, cultivation, and maintenance of non-
pathogenic and pathogenic species
of fungi and yeasts.
• SDA was formulated by Sabouraud in 1892 for
culturing dermatophytes.
• The pH is adjusted to approximately 5.6 in order
to enhance the growth of fungi,
especially dermatophytes, and to slightly inhibit
bacterial growth in clinical specimens.
50. Principle
The SDA media is comprised of enzymatic digest of casein (BHI) and animal tissues which
provide a nutritious source of amino acids and nitrogenous compounds for the growth of fungi
and yeasts.
Dextrose is the fermentable carbohydrate incorporated in high concentration as a carbon and
energy source.
Agar is the solidifying agent.
The addition of antibiotics like Chloramphenicol and/or tetracycline acts as broad-spectrum
antimicrobials to inhibit the growth of a wide range of gram-positive and gram-negative
bacteria.
Cyclohexamide is added to further inhibit the growth of gram-negative bacteria.
51. • Ch
Chloramphenicol – Suppress growth of
contaminating bacteria
Cycloheximide – Suppress growth of
contaminating fungi.
Brain heart infusion (BHI) with blood and
antibiotics – for primary isolation of fungi.
123. Results & interpretation
Fungi Colony morphology
1 Aspergillus flavus Yellow-green, powdery and pale yellowish on reverse
2 Aspergillus niger
The initial growth is white, becoming black later on giving “salt and
pepper appearance” which results from darkly pigmented conidia
borne in large numbers on conidiophores and reverse turning pale
yellow
3 aspergillus fumigatus Blue – green, powdery and pale yellow on reverse .
4 Trichosporon mucoides White to cream, yellowish, wrinkled
131. Objective questions
1. 1. Discuss in detail about laboratory diagnosis of fungi. A . Direct microscopy, B . Culture, C . Tissue sections
2. 2. How would you classify various types of fungal diseases ?
3. 3. Write the types of superficial mycoses
4. 4. Discuss in detail about surface fungal infections. I.e: Tenis vesicolor, Tinea Nigra & Piedra
5. 5. Write the introduction, classification, species, clinical infection, Laboratory diagnosis & treatment of
Dermatophytes
6. 6. Classify various types of subcutaneous mycoses
7. 7. Discuss in detail about Mycetoma
8. 8. What is chromomycosis, discuss about its Lab diagnosis
9. 9. What is sporotrichosis? Discuss it's laboratory diagnosis?
10. 10. Discuss in detail about Rhijosporodiosis.
11. 11. Name some opportunitiatic fungi?
12. 12. Discuss in detail about introduction, morphology, species pathogensis and clinical symptoms of Candidiasis.
13. 13. Discuss in detail about laboratory diagnosis of Candida infection
14. 14. Elaborate on Aspergillosis
15. 15. What are mycotoxins? Give some examples.
16. 16. Writes the names of systemic mycoses.