Microbiology (laboratory diagnosis of viral and fungal infection)

1. Microbiology practical
Laboratory diagnosis of viral & fungal infection
By : Osama Al-Zahrani
@OSAMA_Z96

2. Diagnosis of Viral infection

3. Diagnosis of Viral infection
Marker : what look for.
Technique (Assay or test) : How look for.
Marker:
Sample : Blood serum
ELISA : test for detect virus Antigen.
Example for ELISA :
Detection of Hepatitis B surface Antigen (HBsAg)
Part of virus ( Protein or Nucleic acid )
Foot steps of virus ( Specific Antibodies )

4. Colour indicates presence of
HBsAg in sample. (+ve test)
Absence of colour indicates absence
of HBsAg in sample. (-ve test)
-ve Control
+ve Control
Serum
sample
+ve Sample
-ve Sample
Detection of HBsAg

5. Micro plate

6. Diagnosis of Fungal infection

7. Diagnosis of Fungal infection
Marker : what look for.
Technique (Assay or test) : How look for.
Sample : Skin scraping
Skin scraping for fungi :
Direct microscopy by KOH Preparation :
10% for skin scraping
20% for nails scraping
Culture

8. Moulds

9. Yeast

10. Yeast

11. Hyphae
Spore
MicrosporumEpidermophyton Trichophyton
Moulds mycelial form

12. Fungal element in 10% KOH
Hyphae
Spore