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1. Urine 313 Practical
Session

2. Methods for Urine cultivation:
Method name:
Four quadrant streaking.
Clinical significance:
Isolation of different bacterial
Species in case of Co-infection
UTI;
(Example: E.coli and
Staphylococcus co-infection).

3. Methods for Urine cultivation:
Method name:
Network- streaking.
Clinical significance:
Calculation of CFU/ml of urine
Sample for detection of
Significant bacteriuria ;
(Example: for midstream
catch urine=105 CFU/ml
or more ).

4. Lactose Fermenting Colonies on
CLED agar.
- Gram negative bacilli.
- Possible causative
organisms: E. coli,
Klebsiella, others.
- Further tests for
identification: IMViC
or API 20E.

5. IMViC Test.
- Positive, Positive,
negative, negative → E.
coli.
- Negative, negative,
positive, positive →
Klebsiella spp.

6. Non-lactose fermenting colonies on
CLED
- Possible causative
organisms: Proteus,
Pseudomonas aeruginosa.
- Further tests for
identification: Oxidase test,
urease test, swarming on
blood agar, API 20E.

7. Swarming on blood agar.
- Possible organism:
Proteus spp.
- Other confirmation tests:
API 20 E, urease + test.
- Infections caused by
Proteus spp.: UTI,
bacteremia and septicemia.

8. C. albicans gram stain
• Gram positive budding oval and round yeast-like
cells.

9. Candida albicans on CLED and Sabouraud
dextrose agar
- Rapid confirmation test:
Positive Germ tube test.
- Infections caused by
C. albicans: UTI
(urethritis) and vaginitis.
- Selective media:
Sabouraud dextrose agar.

10. Gram positive cocci in cluster.
- Possible organisms: S.
aureus, S. saprophyticus
- Differentiation test:
Coagulase test.
o Coagulase positive:
S. aureus.
o Coagulase negative:
S. saprophyticus.
Coagulase negative
Coagulase positive

11. Couples of Schistosoma
hematobium.
- Classification:
Trematodes (fluke).
- Infective stage of
schistosoma
hematobium: cercaria.
- Diagnostic stage:
terminal spine ova in
urine.