its all about SCREENING OF B&R LINE OF DEVELOPING HYBRID AGAINST HEAD ROT DISEASE

1. IHSANULLAH
2015-ag-6883
B.Sc (Hons.) Agri. Sciences
8th semester
Plant Pathology Section
Oil Seeds Research Institute
Ayub Agriculture Research Institute
Faisalabad
Mr. AHSAN MOHYO-U-DIN
(Asst. Oilseeds pathologist)
12/17/2020 1

2. Research Title
SCREENING OF B&R LINE OF DEVELOPING HYBRID AGAINST
HEAD ROT DISEASE
Objectives:
 To maintain the genetic stock for their utilization in
synthesis of new sunflower hybrids.
 To test the performance of newly developed sunflower
hybrid.
12/17/2020 2

3. IMPORTANCE OF SUNFLOWER
• 2nd most important oilseed crop
• 17 species cultivated worldwide
• Pakistan
• Production: 40% of total consumption
• Import cost for 60% is Rs. 23020 million
12/17/20203

4. pathogen introduction
“Rhizopus arrhizus”
• Classification
• Specie arrhizus
• Genus Rhizopus
• Family Mucoraceae
• Class Zygomycetes
• Kingdom Fungi
• Air and Soil borne spore
12/17/2020 4

5. METHODOLOGY
•Design R.C.B.D
•Repeated. three
•R×R 75cm
•P×P. 9cm
•Fertilizer 148:99:62N.P.K kg/hectare
•Sowing date 6/2/2019
•Inoculation of disease was done by head injury
method at flowering on head.
12/17/2020 5

6. Plane of Work
IN-VITRO
• Sterilization
• Preparation
• Isolation
• Purification
• Identification
12/17/2020 6

7. 12/17/2020 7
Isolation
Pouring of PDA
Preparation of inoculum

8. Plan of work “in-vivo”
12/17/2020
 Inoculation of head rot disease will be done by Bit
inoculation Method at flowering stage.
 Data will be recorded on the basis of 0-9 rating scale
observing the disease density on the sunflower heads.
Inserted Inoculum moisturing the cotton Watering of cotton after 24 hours

9. DATA COLLECTION
• After 12-15 days at maturity the data was collected on the
basis of disease/healthy plants.
• %age of disease was observed by the formula.
12/17/2020 9
Disease %=
𝑵𝒐.𝒐𝒇 𝑰𝒏𝒇𝒆𝒄𝒕𝒆𝒅 𝑯𝒆𝒂𝒅𝒔
𝑵𝒐.𝒐𝒇 𝑰𝒏𝒐𝒄𝒖𝒍𝒂𝒕𝒆𝒅 𝑯𝒆𝒂𝒅𝒔
× 100
During data collection

10. Results and Discussions
Disease rating Scale for estimation of head rot of sunflower
12/17/2020 10
Score Conditions Remarks
0 No disease Immune
1 1% or less are head rotten Highly Resistant
3 1-10% head rotten Resistant
5 11-25% head rotten Moderately Resistant
7 26-50%head rotten Susceptible
9 51% or above head rotten Highly susceptible

11. SCREENING OF B&R LINE OF DEVELOPING HYBRID
AGAINST HEAD ROT DISEASE OF SUNFLOWER.
12/17/2020 11
Sr.# ENTRIES/LINES R1 R2 R3 MEAN DISEASE% REMARKS
1 ORI-2 5 5 3 4.33 87 HS
2 ORI-26 4 4 5 4.33 87 HS
3 ORI-28 5 4 4 4.33 87 HS
4 ORI-29 4 5 5 4.66 93 HS
5 ORI-30 4 5 4 4.33 87 HS
6 ORI-42 5 4 4 4.33 87 HS
7 ORI-43 5 5 4 4.66 93 HS
8 ORI-44 4 5 5 4.66 93 HS
9 ORI-46 3 5 4 4 80 HS
10 ORI-64 4 4 5 4.33 87 HS
11 ORI-66 4 4 5 4.33 87 HS
12 ORI-76 4 5 5 4.66 93 HS
13 ORI-90 5 5 4 4.66 93 HS
14 ORI-92 5 5 3 4.33 87 HS
15 ORI-100 3 4 5 4 80 HS
16 ORI-101 4 5 5 4.66 93 HS

12. Conclusion
• All 16 ORI B&R lines were highly susceptible.
12/17/2020 12