It will help the researchers how to conduct diallel mating among mustard crops. This study also aims to screen out salinity tolerance and short duration mustard genotypes which will lead to develop a desired variety in future

1. Diallel Mating in Selected Short Duration Oil Seed
Brassica Genotypes and Heterotic Performance of
Hybrids Under Salinity Stress
Presented by:
Md. Fahim Hassan Khan
Roll No. :19GPB-JJ26M
Reg. No. :42917
Supervisor:
Dr. Arif Hasan Khan Robin
Professor and Head
1
Department of Genetics and Plant Breeding
Bangladesh Agricultural University,
Mymensingh

2. Outline
Introduction
Objectives
Materials and Methods
Time Frame and Budgeting
Possible Risk
Conclusion
Acknowledgements
Scholars in this area and References
2

3. Introduction
Mustard and Rapeseed (Brassica
spp.) are one of the most important
oilseed crops
3 U triangle species: B. rapa, B.
Juncea, B. napus are being cultivated
in Bangladesh
3.36 lakh ha produces 3.6 lakh tons
(YAS,2017), 0.5% of World’s total
production
Cultivate in Rabi season between
Boro and T-Aman Rice
Get less time priority as Rice is our
main concern
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Fig1: Brassica U Triangle

4. salinity is a great problem in Bangladesh
and around the world
Approximately 30% cropping area, 1.056
million ha of arable lands in Bangladesh
Crop production is greatly hampered
So, it is crucial to develop salinity
tolerance variety
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Fig2: Salinity effect on Plant

5. 5
 Specific Objectives
To mate selected mustard genotypes following diallel method to
access GCA & SCA
 To investigate morphological and biochemical traits of hybrids
and parents under salinity stress
To screen out short duration and salinity stress tolerant
genotypes
Objectives
Broad objective
To identify short duration and salinity tolerance Mustard
genotypes.

6. Materials and Methods
Experiment 1: Diallel Mating
Plant Materials Collection: Materials
were collected form GPB field Laboratory.
Site: GPB Field Lab
Time of experiment: Nov 2018-Feb 2019
Diallel crosses were made and F1 seeds
have been harvested
 5×5 diallel mating
No. of cross combinations:20
Replication: 5
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original Source Name of
Genotypes
All of the
genotypes
are
advanced
breeding line
of BARI
BD-10108
BD-10110
BD-10112
BD-10455
BD-7113
Table: List of Genotypes
Fig3: Emasculation Fig4: Pollination Fig5: Harvesting

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Experiment 2: Hydroponic culture
under salinity stress
Site: Plant Culture Room, Dept. of GPB, BAU
Time: Jun 2019-Sep 2019
 Activities to be Done..
Parent and Hybrid seeds will be
grown in hydroponic under
different salinity stress
Morphological data for various traits will
be taken
Biochemical analysis will be done
Salinity
level
Hydroponic
culture
0mM Temp: 20±1oC
50mM pH: 5.8-6.3
100mM E.C: 0.7-1
~ proline, hydrogen peroxide, MAD, POX, CAT, APX etc.
Shoot length, Root
length, No. of live leaves,
RDW, SDW, Branching,
Date of 50% flowering

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Experiment 3: Screening of desired genotypes
Site: GPB Field Lab
Time: Nov 2019-Feb 2020
 Activities to be done..
F1 and parental seeds will be shown in field
Design: RCBD
Data will be taken
At vegetative and Reproductive stages
Statistical analysis will be done
Software: Minitab 18, PLABSTAT, R
Shoot length, Root
length, no. of live leaves,
Branching, Date of 50%
and 80% flowering, no.
of fruits per plant, no. of
seeds per fruit, RDW,
SDW, 1000 seed weight

9. 9
Time Frame
Nov 2018 –
Feb 2019
Mar 2019 –
Jun 2019
Jul 2019 –
Oct 2019
Nov 2019 –
Feb 2020
Mar 2020 –
May 2020
Making
Research
Proposal
Genotype
Collection
Diallel
Mating
Literature
Review
Observing
Senior’s
experiment
Literature
Review
Growing in
Growth
Chamber
Hydroponic
Media
Managing
Impose
Salinity
Treatment
Sowing F1
at Field
Selfing &
Data
Collection
F2 Seed
Harvesting
Thesis
Writing
Thesis
Evaluation
Thesis
Submission

10. 10
Budgeting
Items Amount (TK)
Field experiment 10,000
Hydroponic culture 20,000
Chemical analysis 15,000
Others 5000
Total 50,000
Germination failure of seeds
Insect and Pest attack
Weather risk during field experiment
Management risk at field
 System and Electricity risk during Hydroponic experiment
Mechanical and Chemical interruptions
Possible Risks

11. Conclusion
As mustard is a good source of edible oil, its wide cultivation is
necessary. But in Bangladesh Rice is the superior cultivated
crop. Besides, salinity hampers crop cultivation to a great
extent.
So it needs to incorporate a short duration, high yielding, and
salinity tolerance variety in between two major rice growing
seasons.
This study may will help to screen Short duration mustard
genotypes considering Salinity and other traits in future.
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Acknowledgements
 Supervisor: Prof. Dr. Arif Hasan Khan Robin sir
 Sharif Ar Raffi sir
 Field technicians and Stuffs
 Special thanks to Jaber Bin Azim

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Scholars
Adefris Teklewold Chere (senior scientist of CYMMIT)
Prof. Dr. Heiko C. Becker (Georg-August-University, Goettingen, Dep. of
Crop Sciences)
Dr. Arif Hasan Khan Robin (Professor, Bangladesh Agricultural University)
B. Griffing
Helal, M. U., Islam, N., Kadir, M., & Miah, N. H. (2016). Performance of rapeseed
and mustard (Brassica sp.) varieties/lines in North-East region (sylhet) of
Bangladesh. Agril Res Technol, 2, 01-06.
TEKLEWOLD, A., and H. C. BECKER, 2005 Heterosis and Combining Ability in a Diallel
Cross of Ethiopian Mustard Inbred Lines. Crop Science 45: 2629-2635.
Yearbook of Agricultural Statistics-2017
References

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