Improvement Of Oilseeds Crops Through Biochemical Basis Of Resistance
1. Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
College Of Agriculture, Latur
Post – Graduate Seminar Series: 2017-2018
Improvement Of Oilseeds Crops
Through
Biochemical Basis Of Resistance
Deshmukh A. S.
PG- Student
Reg. No. 2016A/28ML
GPB
Seminar Incharge
Dr. P. B. Wadikar
Asst. Prof. Botany
College Of Agri. Latur.
VNMKV, Parbhani.
Research Guide
Dr. A. M. Misal
Jr. Sunflower Breeder
Oilseed Station, Latur
VNMKV, Parbhani.
Head of Department
Dr. V. N. Toprope
Dept. of Botany
College Of Agri. Latur.
VNMKV, Parbhani.
2. 1. INTRODUCTION
2. SCENARIO OF OILSEEDS
3. IMPORTANCE
4. SCOPE
5. TERMS
6. CASE STUDY
7. COCLUSION
8. SUMMERY
CONTENTS
3. INTRODUCTION OF OILSEEDS
India is one of the major oilseeds producing country in the
world in the accounting for about 16% of the area and 10 %
share in production in the world oilseed production.
In India, oilseeds is cultivated on about 25.72 M.ha. area with a
production of 26.775 MT.
It occupies fourth position amongst all vegetable oils producing
countries in the world after USA, China and Brazil.
In India oilseeds occupy nearly 14% of country’s gross cropped
area and contributes to 5% of the GNP and 10% value of the of
agricultural products.
Source : Oilseeds statistics, IIOR, Hyderabad, 2015
4. WORLD POSITION IN OIL SEED
202.29 199.53 208.95
220.36
416.77
393.1
447.8
474.57
20.6 19.7 21.43 21.54
2011-12 2012-13 2013-14 2014-15
Area in MHa. Production in MT. Productivity kg/ ha
http;/faostat3.fao.org
5. INDIA POSITION IN OIL SEED
263.08 264.84
280.84
257.26
297.99
309.4
327.49
266.75
11.33 11.68 11.68 10.37
2011-12 2012-13 2013-14 2014-15
Area in Mha. Production in MT. Productivity in Kg/ha
Directorate of economics and statistics
http:/eands.dacnet.nic.in
6. MAHARASTRA POSITION IN OIL SEED
3.66 3.8 4.14 4.3
4.48
5.08 5.29
2.83
12.23
13.37
12.76
6.58
2011-12 2012-13 2013-14 2014-15
Area In MHa Production in MT. Productivity in kg/ha
Directorate of economics and statistics
http:/eands.dacnet.nic.in
7. RANK OF OIL SEED IN INDIA IN
AREA & PRODUCTION
43.09
22.51 21.46
6.91
3.86
2.14 0.91
39.47
23.65 24.58
3.04
0.57 1.56 0.27
Area in (%) Production (%)
Directorate of economics and statistics
8. MAJOR CONSTRAINTS
FOR LOW YIELD
Market oriented cultivation.
Predominantly rainfed cultivation, poorly distributed
rains during cropping season.
Improper rain water management, lack of irrigation
facility even for protective irrigation during dry spell.
Imbalanced / low fertilizer management.
Non-adoption of improved water management
practices.
Use of non descript genotypes.
Improper crop rotation.
9. OILSEEDS SCENARIO IN INDIA
India ranks first in the world in terms of area.
Oilseeds is produced in India in eight growing states of
India.
Major oilseeds growing states in India MP, RJ, MS, GJ,
AP, KA respectively.
Vegetables oils product contribute highest value in
Agriculture imports.
11. IMPORTANCE OF OIL SEED
Vegetable oil is necessary part of our diets.
In terms of vegetables oils, India is the fourth largest oil
economy in the world after USA, China and Brazil.
The oilseed cultivation also provides employment to
rural people (35 million in oilseed production) and 1
million in their processing.
Oil cake is use as cattle feed and fertilizer.
www.agri.info.in
12. Linseed oil has great demand in industries purpose
as lubricant and as row material
Caster cake controlled termites due to its vermicide
property.
Oilseeds are the good source of foreign exchange.
Non-edible cake use as the farm manure.
www.agri.info.in
13. NUTRITIONAL VALUE OF OILSEEDS
Edible oils are the concentrated source of energy,
the energy content of the oil is much higher (39.80 mg/kg)
than protein (23.88 mg/kg) or carbohydrate (16.76 mg/kg)
Sr.
No
Constitute Fatty acids
1 Carbohydrates SFA Remarks UFA Remarks
2 Essen. Fatty acids Palmitic Harmful Oleic Beneficial
3 Vitamins(A,D,E,K) Stearic Harmful Linoleic Beneficial
4 Proteins Linolenic Beneficial
5 Erucic Harmful
14. SCOPE IN OILSEED
Imports: are the product or raw material brought from another
country for use in your own country.
Year Import
in
MT.
Import in
rupees
(crore)
% share of
Agri.
product
% share of
oil in total
Agri.
product
2010-11 6.90 29860 3.4 52
2011-12 8.45 46255 3.5 56
2012-13 11.01 61107 4.1 56
2013-14 10.43 56488 3.9 54
Agricultural statistics at a glance and DGCIS , Kolkata
15. Climatic condition in India :
1. Total agro climatic zones -15
2. Agro. Ecological zones -21
Rain fed area:
More than 85 % oilseeds area under rain fed area.
For oil seeds production less water is required.
Mostly raised on marginal and sub marginal land.
(farmers not well adapted to new farm technology).
Lack of HYV/hybrid having high oil %.
Varieties are high yielding but susceptible to disease & pest.
16. Strategies for increasing oil seeds production
•Bringing more area under oilseed.
• Good quality seed for recommended varieties for
specific area and season.
•Use HYV and hybrids.
• Adoption of improved production technology .
•Strengthening PHT facilities
• Develop resistant cultivar.
17. BREEDING
Definition : Evaluations of crop directed by man (Vavilov)
Objective : 1. Yield
Many Breeder produce HYV, but during crop life cycle
many diseases and pests are incident.
Our plant protection department trying to develop new
pesticide, insecticide, fungicides.
For result farmer use this chemical in high concentration
compare to recommendation given by the company.
Hazards effect of this chemicals are injurious to health.
19. IMPORTANT DIEASES AND PEST OF
OILSEED CROPS
Crop Pest Disease
Sunflower Hairy caterpillar
Head borer
DM. , Leaf blight, PM.,
Rust,
Sesame Sesame gall fly,
Til hawck moth
PM., Phyllody,
Leaf blight, Leaf spot.
G-nut Aphid,
Leaf miner
Tikka,
Bud necrosis
Soybean Aphid, Jassid
pod borer
Rust, Anthracnose,
Bacterial blight
Mustard Bihar harry caterpillar
Mustard Aphid, Saw fly.
Leaf blight,
White rust, PM.
Linseed Linseed gall fly
Linseed caterpillar
Rust,
Wilt
Castor Castor semi looper,
Castor capsule borer
Rust, Leaf blight,
Brown leaf spot
20. BIOCHEMICAL RESISTANCE
Resistance :- the ability of plant to withstand completely
or in some degree, the effect of a pathogen or other
damaging factor.
Biochemical :- chemical present inside the plant body
naturally.
Different biochemical : -
1. Phenol
2. Poly-phenol
3. Peroxidase
4. HCN
5. Sugar
6. Catalase
21. MACHANISM OF BIOCHEMICAL
IN RESISTANCE
PHENOL :-
Is aromatic organic compound
Also called as Carbonic acid,
With the molecular formula C6H5OH
It is the white crystalline solid that is
volatile.
POLYPHENOL :-
Made up of large number of the phenol units.
They are mainly natural but can be made by
synthetically or semi-synthetically.
Eg.- Catchin,
Anthocyanin,
Resveratrol.
OH
22. Peroxidase :-
Antioxidant defense mechanism.
This are the large family enzymes.
Carries the biological reactions in the living body.
Play role in conversion of the H2O2 to H2O
HCN :-
May play a dual role in plant.
At high concentration it play role as defense mechanism.
At low level concentration it play role in metabolism.
24. CASTOR
1st
Research on :-Biochemical Basis Resistance To Leaf miner in Castor (Ricinus communis L.)
K Anjani*, and M. Pallavi, S. N. Sudharkara Babu
Directorate of oilseed research, Hyderabad.
Introduction :- India is the major growing country in the world
Objective :- Role of Total phenol in leaf miner resistance.
Material and Methods :- Researcher selects the two parent in which
RG-1930 (dark purple) resistant to leaf miner.
RG-2788 (green color) suitable to leaf miner.
Makes the reciprocal cross and screen the F1, F2, F3. generation
Also analysis the total phenol from each generation by
spectrophotometer (Price and Butler, 1977).
For screening each parent was planted in 6 row and
In F1, F2 and F3 generation (R x S) planted in 33, 60, 18 rows and
the (S x R) is planted in 23, 57, 17 rows
Anjani et al.
26. Parent and different
phenotype in of R x S
and S x R crosses
Number of
plants
Number of
mines/leaf
Heavily infested
leaves/plant (%)
Reaction against
leaf miner
Mean Range Mean Range
Parent
Rg-1930
RG-2788
50
50
0.6
103
0-4
85-157
0
87
-
82-90
R
S
R x S
Dark purple
Purple- green
832
257
1.8
52
0-8
31-54
0
55
-
51-57
R
S
S x R
Green 957 104 85-156 86 76-95 S
Means and ranges of mines/leaf and heavily infested leaves/plant(%) in
parents phenotypes in R x S and S x R crosses.
Anjani et al.
27. Parents and
crosses
Leaf color Reaction against
leaf miner
Number of plants
analyzed
Total phenol
concentration* (C.E.)
Mean Range
Rg-1930 DP R 50 2.31 2.22-2.41
RG-2788 G S 50 0.23 0.21-0.24
R x S cross DP
PG
R
S
170
129
2.17
0.54
2.10-2.22
0.45-0.64
S x R Cross G S 170 0.21 0.14-0.26
Total phenol concentration in parent & different leaf color offspring observed in R x S and S x R cross
Different generation of R x S
and S x R cross
Correlation coefficient between
Total phenol concentration and
no. of mines/ leaf
Total phenol concentration and
heavily infested mines/plant (%)
R x S
F1
F2
F3
-0.98**
-0.95**
-0.92**
-0.99**
-0.97**
-0.95**
S x R
F1
F2
F3
-0.97**
-0.96**
-0.98**
-0.99**
-0.96**
-0.98**
Correlation of Total phenol concentration
Anjani et al.
28. RESULT
Purple –green leaf plants exhibited comparatively lesser
leaf miner infestation than the green leaf plants.
Total phenol concentration showed significant negative
association with number of mines/leaf.
Anjani et al.
29. . Biochemical basis of disease resistance in Linseed (2000)
Jyoti Singh and Vajpeyi
30. Research on :- Biochemical basis of disease resistance in linseed (2000)
Jyoti Singh and Vajpeyi
C. S. Azad univercity of agriculture & Technology
Objective :- Investigate the role of biochemical HCN, Polyphenols,
Nitrogen, Phosphorus, Sugar %.
Material and Methods :- Study 20 genotypes out of which 19 are
L. usitatissimum and one L. grandiflorum
Collect the sample of leaf on
60 DAS :- pre-infection
110 DAS :- post infection
Analysis the HCN, Polyphenols, Nitrogen,
Phosphorus, Sugar %.
Linseed 2nd
Jyoti et al. (2000)
32. RESULT
AYOGI, BAU-610, KIRAN and L. Grandiflorum
contained high percentage of HCN, Polyphenol,
Phosphorous causes disease resistance
C-429, CHMBAL contained high level of Sugar
Nitrogen percentage causes increase in disease infection.
Jyoti et al. (2000)
34. MUSTARD
3rd
Research on :-Biochemical Basis Resistance Against Powdery Mildew
Of Mustard
Shubhangi A. Mohitkar , A. G. Gathe, S. B. Bramhankar* and G. D. Mate
College of agriculture, Nagpur
Objective:- Investigate the role of biochemical
Material and Methods :- Collect the leaf sample on
30 DAS (Healthy) and
60 DAS(Diseased)
Analysis the phenol, polyphenol,
peroxidase, catalase
Mohitkar et al.
36. RESULT
Accumulation of phenol increase synthesis and
translocation of phenol content at the site of
infection and release glycoside either in host or
pathogen.
The increase in level polyphenol may be due to
accelerated synthesis of phenol.
conversion of total phenol in quinines which
were toxic to the growth of pathogen.
Mohitkar et al.
37. .
Differential Expression of sunflower Peroxidase Isoforms and
Transcripts during Necrotropics Interaction with
Alternaria helianthi
G. Anjana K. R. Kini et al
38. Research on :- Differential Expression of sunflower Peroxidase
Isoforms and Transcripts during Necrotropics
Interaction with Alternaria helianthi.
` G. Anjana K. R. Kini, H. S. Sheety and H. S. Prakash.
University of Mysore
Introduction :- Sunflower leaf spot is destructive foliar disease cause
by Alternaria helianthi.
Material and Methods :- They select two genotype
MSH-59 (resistance) and MODREN (Susceptible) are
grown in green house conditions.
conidial suspension of A. helianthi at concentration of
106 conidia/ml is spray.
Collected sample is analysis by 8% PAGE.
Estimate the Peroxidase at 0, 2, 3, 9, 12, 24, 48 & 72hr.
SUNFLOWER 4th
Anjana (2007) et al.
40. RESULT
Differential accumulation of peroxidase isoforms and the
transcripts in resistance and susceptible genotypes of
sunflower upon inoculation with A. Helianthi.
Peroxidase isoforms and gene expression are regulated by
the pathogen stress.
Anjana (2007) et al.
41. Biochemical changes in relation to
Alternaria leaf blight in Indian
Mustard
A.k. Atwal et al.
42. Research on :-Biochemical changes in relation to Alternaria leaf
blight in Indian Mustard
A.k. Atwal Ramadeep, S.K. Munshi, P. S. Mann
Punjab agriculture University, Ludhiana.
Objective :- To investigate the role of sugar, total phenol, Flavonols,
Chlorophyll against Alternaria leaf blight in Indian
Mustard.
Material and Methods :-Healthy and naturally infected leaves of
mustard were collected from lower middle and
upper portion of the plants at 80, 100 and 120
DAS.
Analyze the biochemical parameters.
CASE STUDY 5th
Atwal (2005) et al.
43. Days after
Soweing
Infection (%) Severity Leaf dry wt. (gm)
Healthy Infected
80 18 15 0.57 0.51
100 49 56 0.89 0.42
120 89 91 0.92 0.23
Percentage infection, Severity of A. blight and dry wt.
of healthy and infected leaves
Atwal (2005) et al.
45. Assessment of yield & late leaf spot
Disease Resistance components in G-nut
Rathod S. S. and Toprope V. N.
46. CASE STUDY 6th
Research on :- Assessment of yield & late leaf spot Disease
Resistance components in G-nut
Rathod S. S. and Toprope V. N.
Objective :- Investigate the role of biochemical
Material and Methods :- Study the 18 genotypes for variability and
the biochemical parameter for disease
resistance.
Analyze the LLS severity (%),
SCMR,
Non reducing sugar,
reducing sugar,
Total sugar.
Rathod et al.
47. Analysis of variances for Biochemical
parameter
Source Mean sum of squares
Replication Treatment Error
LLS severity (%) 1.311 888.93** 1.424
SCMR 1.115 46.043** 1.983
Non Reducing
Sugar (mg/g)
0.0896 28.528** 0.115
Reducing
Sugar (mg/g)
0.0187 1.968** 0.006
Total Sugar (mg/g) 0.038 15.659** 0.109
Pod yield (gm) 3.970 13.432** 2.842
* Significance at 5%, ** significance at 1%.
Rathod et al.
48. Character LLS
severity
SCMR N.R. sugar R. Sugar Total sugar Pod yield /
Plant(gm)
LLS
severity
G
P
1
1
-0.7761*
*
-0.7263**
-0.8514**
-0.8492**
0.8333**
0.8321**
-0.8540**
-0.8505**
-0.9150**
-0.7971**
SCMR G
P
1
1
0.5180**
0.4844**
-0.5520**
-0.5113**
0.5036**
0.4722**
0.6401**
0.4412**
N.R.
sugar
G
P
1
1
-0.9909**
-0.9883**
0.9989**
0.9985**
0.9520**
0.8404**
R. Sugar G
P
1
1
-0.9833**
-0.9783**
-0.8623**
-0.8110**
Total
sugar
G
P
1
1
0.9761**
0.8461**
Genotypic & phenotypic correlation to
Biochemical parameters
Rathod et al.
49. Characte
r
LLS
severity
SCMR N.R. sugar R. Sugar Total
sugar
Pod yield /
Plant(gm)
LLS
severity
G
P
0.5057
0.0078
-0.1687
-0.0057
-0.2819
-0.0067
0.2547
0.0065
-0.2860
-0.0067
-0.9150**
-0.7971**
SCMR G
P
-0.2624
-0.0033
0.3381
0.0046
0.1751
0.0022
-0.1866
-0.0024
0.1703
0.0022
0.6401**
0.4412**
N.R.
sugar
G
P
0.7948
0.7867
-0.4836
-0.4487
-0.9336
-0.9263
0.9251
0.9155
-0.9325
-0.9250
0.9520**
0.8404**
R. Sugar G
P
-0.1299
-0.2077
0.0860
0.1276
0.1545
0.2467
-0.1559
-0.2496
0.1533
0.2443
-0.8623**
-0.8110**
Total
sugar
G
P
-0.6859
-0.5840
0.6403
0.3242
0.8700
0.6857
-0.2502
-0.6720
0.9715
0.6867
0.9761**
0.8461**
Path coefficient analysis with
Biochemical parameter
Rathod et al.
51. CONCLUSION
During the parent selection for the
breeding of oilseeds crop we must
consider the Biochemical parameters for
development of superior lines.
Also for developing the bio fortification
food.
52.
53. LITERATURE CITED
Agricultural statistics at a glance and DGCIS , Kolkata
Directorate of economics and statistics
Oilseeds statistics, IIOR, Hyderabad, 2015
Biochemical basis of disease resistance in linseed (2000) Jyoti Singh and Vajpeyi C.
S. Azad univercity of agriculture & Technology
Differential Expression of sunflower Peroxidase Isoforms and Transcripts during
Necrotropics Interaction with Alternaria helianthi.G. Spriger book chapter (2005)
Anjana K. R. Kini, H. S. Sheety and H. S. Prakash. University of Mysore
Biochemical changes in relation to Alternaria leaf blight in Indian
Mustard Springer chapter (2015) K. Atwal Ramandeep, S. Munshi, P. S. Mann
Punjab agriculture University, Ludhiana.
Assessment of yield & late leaf spot Disease Resistance components in G-nut
Rathod S. S. and Toprope V. N.