New breeding tool i.e. speed breeding (indoor plant breeding technique)to enhance the life cycle of crop. The concept involves exposing plants to an inordinate amount of light in a close green house environment in an efforts to speed up the growing process and produce new generation of seeds in a much quicker fashion

1. W
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3. Presented by
SMITA SUNILRAO DESHMUKH
Reg. no.2019A30M
Research Guide
Dr. R.R.Dhutmal
Seminar Incharge
Dr. H.V.Kalpande
Dr. M.P.Wankhade
VASANTRAO NAIK MARATHWADA AGRICULTURE
UNIVERSITY, PARBHANI
2020

4. CONTENT
 Introduction
 Story so far
 Concept
 Why speed breeding?
 Core recipe of speed breeding
 Photoperiodism & temp. plays role…
 Methods of speed breeding
 Application
 Benefits of speed breeding in plant research
 Case study
 Achievements
 Limitation
 Challenges ahead
 Conclusion

5. INTRODUCTION
 Growing human population and a
changing environment
 Need of speed

6. Story so far…
 Plants in space
 Outer space origin
Zinnia plant in earth orbiting space
station

7. CONCEPT
•SPEED BREEDING - An indoor plant growing
technique.
The concept involves exposing plants to an
inordinate amount of light in a close green house
environment in an efforts to speed up the growing
process and produce new generation of seeds in a
much quicker fashion.

8. Dr Lee Hickey is a plant
breeder and crop geneticist
within the Queensland Alliance
for Agriculture and Food
Innovation at The University of
Queensland, Australia.
Amy Watson B. H. Wuff Sreya Ghosh

9. Why Speed Breeding?
Crossing&
inbreeding
Testing-Yield
Disease,
quality
Seed increase &
release
3-7yrs 4-5yrs 1-3 yrs
Testing-Yield
Disease, quality Seed increase &
release
Crossing&
inbreedin
g
1-
2yrs
4-5yrs 1-3yrs
SPEED
BREEDIN
G

10. Core Recipe
 Light - Continuous light =continuous
growth?
PAR region 400-700nm
Light emitting diode
 Photoperiod -
22hrs 2hrs
 Temperature -
 Humidity - 60-70%
LIGHT
Darknes
s
22°C 17°C

11. Photoperiodism & Temp. plays
role..
 Photoperiod – The plants in order to
flower require a certain day length i.e.
the relative length of day and night
which is called as photoperiod.
 Photoperiodism – The response of
plants to the photoperiod expressed in
the form of flowering is called as
photoperiodism.

12. Depending upon the duration of the
photoperiod plants are classified into 3 classes

14. Temperature
 It is one of the most important ecological
factors
 It regulates many physiological
processes of the plant.
 The metabolic processes are low at
certain temp.& increase at particular
temp. it is called as optimum
temperature.
 The plant grows best at Optimum
temperature.
 Metabolism again decrease at max.
temp. the plant can not survive above
optimum temp.
 Both extremely low and high temp. have
adverse effect on plant growth.

15. Physiological process which are
affected by temperature
Photosynthesis

16. Temperature also affects
 Spreading of diseases
Germination of
seed

17. Methods of Speed breeding
1.Controlled environment chamber
speed breeding
 Conviron BDW chamber
 Mixture of white LED, far red LED lamps &
metal HQI lamps
 Light intensity 360 – 380 µmol m-2 s-1 at
bench height & 490 – 500 µmol m-2 s-1 at
adult plant height

18. Continue…
2.Glasshouse speed breeding
condition
 Temp. controlled glasshouse fitted with high
pressure sodium vapour lamps
 Temp. with 12hr. Turnover and 22hr
photoperiod
 Light intensity 440-650 µmol m-2 s-1
Photo credit- University of
Queensland, Australia

19. Continue…
3.Homemade growth room design for
low cost speed breeding
 3m×3m×3m room with insulated sandwich
paneling fitted with 7LB-8LED light boxes
 1.5hp inverter
 Light intensity 210-260 µmol m-2 s-1 at bench
height & 310-510 µmol m-2 s-1 at 50 cm above
pot
 Lighting was set to run 12hr photoperiod(12 hr
darkness) for 4 weeks and then increase to an
18 hr photoperiod (6hrs darkness)
 Air conditioner set to run at 18°C in darkness &
21°C when LED on run

20. Application
 Can study physiological traits
 Ideal for generating large breeding
population
 Boosting transgenic & CRISPER
pipelines
 Speed up genomic selection

21. Benefits of Speed breeding for
plant research
 It is affordable method
 Shortened generation
 Runs within minimum place
 Large population can be handled
 Reduced maintenance cost of
developing inbreed lines
 Maintain high degree of homozygosity

22. CASE STUDY
Objective- To reduce release time of new
peanut varieties.
INTRODUCTION-
Reducing the period of time
required to develop new commercial cultivars is of
considerable interest to plant breeder across peanut
breeding programme. New speed breeding technique
which combines controlled environment condition,
continuous light in conjunction with optimal temp. and a
SSD breeding strategy in glasshouse.

23. Materials &
Methods
Two preliminary trials
 Used breeding line (cv.D136-p7-5)
 Light- 450 watt PAR
 Temp. - 32-17°c
 Humidity - 65%
 Pot size - 12×30 cm
 Potting Media – consist of two layers , a lower section of two
parts of karsnozem to one part alluvial sand and upper
section pasteurized peat mix consisting of nine part alluvial
sand, six part peat moss and one part krasnozem
 For preliminary photoperiod trial three suspected differing
photoperiod insensitivities were used
1. Sutherland, runner type
2. Wheeler, Virginia type
3. TAG-24

24. Continue…
F2 &F3 Generation Experiment
• 400 F2 seeds were collected from
cross of one cultivar
Farnsfield × D147-p3-115
• 270 F3 seeds collected from F2
generation

25. Results
Preliminary Experiment
 Of the 120 plants harvested only six did
not produce a single pod
Three Cultivar
FLOWERING
SU – 25DAS
WH – 27DAS
TAG – 24DAS

26. F2 &F3 Generation Experiment

27. Conclusion
 Clearly shows that generation time
can be reduced within a speed
breeding SSD system and hence
new varieties could be developed up
to two years quicker compared to
using conventional field based
pedigree breeding strategies

28. Objective - To access of speed breeding for
multiple quantitative traits
Introduction – Durum wheat is one of the
major crop in Mediterranean region . The no.
of constraints limiting the production like
fungal diseases, insufficient water availability

29. Materials & Methods
The breeding strategy for applying selection in early segregating generations to reach superior
inbreeds in a period of 11 months. The figure highlights the crossing parents and further
generations where a weighted SI was used. The blue colour indicates generations where the
phenotyping was conducted. The green coloured generations indicate the generations subject to
growth under speed breeding for the entire cycle without selection

30. Visual summary of a generation from sowing to harvest using the multi-trait
phenotyping procedure: a seeds sown in the clear-pot, b seminal root image
analysis, c plants inoculated with leaf rust using airbrush method, d plants
inoculated with Fusarium crown rot, e plant height measured using a barcode
reader, and f whole-pot view at the time of crown rot assessment during the
grain filling stage

31. Result
 F2 screening and population
distribution

32. Screening of Selected & Unselected F3
individuals
Density distribution and comparison of population means for selected and
unselected F3 population sets for the following traits: a root angle, b root
number, c crown rot severity, d leaf rust severity and e plant height. Each
set includes 100 F families

33. Conclusion
 The novel multi-trait phenotyping
method presents useful tool for rapid
selection of early generation
 Results suggest that this technique is
compatible with speed breeding &
made it possible to conduct up to four
consecutive screens annually
compared to single screen in field.

34. ACHIEVEMENT

35. Continue…
 DS Faraday - pre- harvest sprouting
tolerance variety

36. Limitations
 Mechanical faults may arise
 Limited to photo insensitive plants

37. Challenges Ahead
 Yet to apply short day species like
rice, maize & sorghum

38. Conclusion
 Novel breeding approach
 Powerful tool to accelerate research
and breeding

39. References
 Watson A, Ghosh S, Williams M, Cuddy WS, Simmonds
J, Rey MD, Hatta MAM, Hinchliffe A, Steed A, Reynolds
D, Adamski N, Breakspear A, Korolev A, Rayner T,
Dixon LE, Riaz A, Martin W, Ryan M, Edwards D, Batley
J, Raman H, Carter J, Rogers C, Domoney C, Moore G,
Harwood W, Nicholson P, Dieters MJ, DeLacy IH, Zhou
J, Uauy C, Boden SA, Park RF, Wulff BBH, Hickey LT
(2018) Speed breeding is a powerful tool to accelerate
crop research and breeding. Nature Plants (4) 23–29
 http://hickeylab.com/our-projects/speed-breeding
 Speed breeding to hasten development of new crop
varieties TV Jayan New Delhi | Updated on March 05,
2019
 https://www.talkplant.com/100-word-explainer-
speed-
breeding/#:~:text=Speed%20breeding%20is%20an
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Fday.