To determine the anti-oxidant property of plant extract Seeking for a new anti-oxidant entity from Bauhinia acuminata Exploration of possible newer medicinal activities of Bauhinia acuminata.

1. WELCOME
TO
MY PRESENTATION

2. Presented by
Farhana Akter Joty
Phytochemical screening and evaluation of antioxidant
activity of methanolic extract of Bauhinia acuminata L.
leaves(Family: Fabaceae)

3. Objectives of the study
To determine the anti-oxidant property of plant extract
Seeking for a new anti-oxidant entity from Bauhinia
acuminata
Exploration of possible newer medicinal activities of
Bauhinia acuminata.

4. Plant Name
Scientific Name: Bauhinia acuminata
English Name: Dwarf white bauhinia, white orchid-tree, snowy orchid-
tree
Bengali Name: Shetokanchon
Taxonomical Classification
Kingdom Plantae
Class Magnoliopsida
Order Fabales
Family Fabaceae
Genus Bauhinia
Species B. acuminata

5. Plant Part Used
Leaves
Images of different parts of Bauhinia acuminata
Leaves Flower Whole Plant

6. Collection of the whole plants of B. acuminata
Shade drying
Milling
Powder of plants
Macerated with methanol for 15 days
Filtration by cotton plug
Crude methanol extract Residue maceration with methanol
for 3 days
Evaporation of the solvent
Filtration by cotton plug
Crude methanol extract
Evaporation of the solvent
Dried Crude extract
Figure: Preparation of crude extract
Extraction at a glance

7. Experimental Method
 Free Radical Scavenging Assay Methods
 Colorimetric Assay For Total Flavonoid Content Determination

8. RESULTS AND DISCUSSION

9. Results Of Phytochemical Tests
Test Result
Alkaloid +
Carbohydrate +
Glycoside +
Steroid +
Tannin +
Flavonoid -
Terpenoid +
Reducing Sugar +
Phenolic Compound +
(+) = Presence (-) = Absence

10. Test for Quantitative Anti-oxidant activity
Table: Data for standard (Ascorbic Acid) and extract of B. acuminata
Blank absorbance = 0.718
Standard (Ascorbic Acid) Extract (B. acuminata)
Concentration
(µg/ml)
Absorbance
(nm)
% inhibition Absorbance
(nm)
% inhibition
1 0.689 4.03 0.638 11.56
5 0.548 23.68 0.589 17.97
10 0.451 37.19 0.501 30.22
50 0.315 56.12 0.278 61.28
100 0.201 72.00 0.109 84.82
500 0.801 88.71 0.086 88.02

11. Ascorbic acid
y = 12.455ln(x) + 24.224
R² = 0.914
Bauhinia acuminata
y = 14.593ln(x) + 2.9155
R² = 0.9133
0
10
20
30
40
50
60
70
80
90
100
0 200 400 600
%DPPHinhibition
Concentration (µg/ml)
Antioxidant activity of Ascorbic acid and
Bauhinia acuminata
Ascorbic acid
Bauhinia acuminata
Log. (Ascorbic acid)
Log. (Bauhinia acuminata)
Figure: Antioxidant activity of Ascorbic acid and Bauhinia acuminata

12. Results of Test for Quantitative Anti-oxidant activity
The IC50 value of ascorbic acid was 7.8µg/ml and extract 25.02µg/ml.
Table: The IC50 values
Ascorbic Acid 7.8µg/ml
Bauhinia acuminata 25.02µg/ml

13. Results of total flavonoid content
Table: Quercetin Standard Calibration
Curve Data
Concentration
(mg/ml)
Absorbance
100 0.069
200 0.127
300 0.268
400 0.379
500 0.433
y = 0.001x - 0.0388
R² = 0.9779
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
0.5
0 200 400 600
Absorbance(nm)
Concentration (mg/ml)
Quercetin Standard Calibration Curve
Absorbance Linear (Absorbance)
Figure: Total flavonoid content determination of
Bauhinia acuminata with the help of Quercetin standard
calibration curve.

14. Extract absorbance 0.769
Discussion
The flavonoid content found in the methanol extract was 807.8mg QE/g of
dried plant material. It was expressed as mg QE/g of dried plant material
using a linear calibration curve equation of quercetin, y = 0.001x - 0.0388
with coefficient of Determination, R² = 0.9779, Where y is absorbance of
methanol extract at 750 nm and x is total flavonoid content in the methanol
extracts of Bauhinia acuminata.

15. Conclusion
The tests carried out for the assessment of phytochemical and antioxidant activities
of Bauhinia acuminata methanol extract revealed the following findings :
 The phytochemical tests showed the presence of reducing sugar, glycosides,
alkaloids, terpenoids, carbohydrates, steroid and tannin.
 In the quantitative antioxidant activity assay, the IC50 of Bauhinia acuminata
extract was 25.02 µg/ml.
 The total flavonoid content was found in the methanol extract was 807.8mg QE/g
of dried plant material.
Finally, it can be said that Bauhinia acuminata extract demonstrated potential
antioxidant activities. So, further investigation might be conducted using pure
compounds of this extract which will determine the responsible compound for these
pharmacological effects.

16. Thank You ...