3. Evaluation of in-vitro antioxidant and in-vitro
antimicrobial activity of methanolic extract of
Mimosa invisa (leaves)
4. Description :
Mimosa invisa is a fast-growing a scrambling climber,
which can form dense thickets in a short span of
time. It is an annual, although behaves as a
perennial. Leaves are bright green, alternate, each
leaf with about 20 pairs of small leaflets, opposite,
6-12 mm long and 1.5 mm wide, sensitive to
disturbance.
Distribution :
Native to South and Central America. Its found in Indian
sub continent, Malaysia, Hong kong, Indonesia, Sri
lanka , Philippines. It is also found in forest of
Chittagong , Coxsbazar , Rajshahi and Bandorban .
5. Plant taxonomy :
Kingdom Plantae
Phylum Spermatophyta
Subphylum Angiospermae
Class Dicotyledones
Order Fables
Family Fabaceae
Genus Mimosa
Species Mimosa invisa
Figure: Mimosa invisa
6. Local name :
Sada sorminda patha , boro lozzaboti , sada lozzaboti
.
Traditional uses :
The plant’s root paste with goats milk is used in treatment of insanity .
It is also used to treat impotency , skin disease , inflammation
,dysentery and burning sensation .
Literature review :
1. Faruque (2007) reported that the plant of Mimosa invisa is used to
treat insanity.
2. Faruque (2007) also reported that it is used to treat impotency.
3. Rahman (2014) reported that skin disease is treated by this plant.
4. Meenatchisundaram S(2009) examined that Mimosa pudica which
is another member of this family has anti venom activity.
7. Literature review revealed that the plants of, Mimosa invisa are
used in different tribes as well as some areas of Rajshahi and
Bandorban without scientific basis or safety concerns.
Determination of its antioxidant and antimicrobial activities
will provide supportive evidence in favor of continuous usage.
The objectives of my project work is
In-vitro antioxidant activity assay
In-vitro antimicrobial activity assay
8. Collection and proper identification of the
plant
Preparation of plant materials
Extraction
In-vitro antioxidant assay
In-vitro antimicrobial assay
9. Plant materials were collected from the forest of Bandarban
district, Bangladesh and authenticated by Dr. Shaikh Bokhtear
Uddin, Professor, Department of Botany, University of
Chittagong.
10. The plant materials were collected in fresh condition. Then
these are cut into small pieces to make it suitable for grinding
purpose and dried. The materials are grinded into coarse
powder with the help of a grinder and stored in an air tight
container for further use
11. The coarse powder was extracted with Methanol. The powder
was submerged in a clean flat bottomed glass container, for
few days with occasional shaking and stirring. The content
was then filtered through several means, e.g. cotton, clothe,
filter paper etc. to get the pure extract
12. DPPH scavenging assay
Iron reducing assay
Determination of total phenol content
Determination of total flavonoid content
13. Concentration
(µgm/ml)
% of scavenging of
ascorbic acid
% of scavenging of
methanolic extract of
Mimosa invisa
20 91.517 42.091
10 88.560 33.192
5 60.544 26.046
2.5 37.198 20.389
1.25 29.494 14.863
14. y = 3.2983x + 35.901
R² = 0.7784
y = 1.3549x + 16.816
R² = 0.9329
0
20
40
60
80
100
120
0 5 10 15 20 25
DPPH scavenging
% of scavenging of AA % of scavenging of MMI
Linear (% of scavenging of AA ) Linear (% of scavenging of MMI)
15. IC 50 of ascorbic acid IC 50 of extract
4.275 24.513
IC 50 of AA
IC 50 of MMI
0
5
10
15
20
25
30
19. y = 0.0039x + 0.033
R² = 0.9987
0
1
2
3
0 100 200 300 400 500 600
Absorbance Calibration curve of Gallic acid
Absorbance of Gallic Acid Linear (Absorbance of Gallic Acid)
Sample
solution
(µg/ml)
Weight of
dry
extract
per ml
m(gm)
Absorbance GAE
conc.
C
(µg/ml)
GAE
conc.
C
(mg/ml)
TPC as
GAE,
A=(Cv)/
m
(µg/ml)
Mean±
SEM
1000 0.001 0.262 76.33 0.08 38.17 36.56
1000 0.001 0.241 69.33 0.07 34.67 ±
1000 0.001 0.254 73.67 0.07 36.83 1.02
20. Concentration (µgm/ml) Absorbance of quercetin
100 0.995
50 0.344
25 0.196
12.5 0.116
0.995
0.344
0.196
0.116
0
y = 0.0098x - 0.0366
R² = 0.9723
-0.2
0
0.2
0.4
0.6
0.8
1
1.2
0 20 40 60 80 100 120
Absorbance
Calibration curve of Quercetin
Absorbance of Quercetin Linear (Absorbance of Quercetin)
21. Sample
solution
(µg/ml)
Weight of dry
extract per ml
m(gm)
Absorbance QE conc.
C
(µg/ml)
QE conc.
C
(mg/ml)
TFC as QE,
A=(C*V)/m
(µg/ml)
Mean±
SEM
1000 0.001 0.513 57.6 0.0576 28.8 28.10
1000 0.001 0.488 55.1 0.0551 27.55 ±
1000 0.001 0.496 55.9 0.0559 27.95 0.37
Chart for the determination of total flavonoid
content :
22. The plant extract shows a significant IC50 values in compare to
standard ascorbic acid were 24.513µgm/ml and 4.275µgm/ml
in DPPH scavenging assay.The plant extract also showed a
significant iron reducing activity .Total phenol content of the
plant extract was 36.56±1.02 µgm/ml and the total flavonoid
content was 28.10±0.37 µgm/ml. From above observation it
appears that that the plant extract has a moderate antiradical
activity.
23. The antimicrobial activity was assayed by disc diffusion method
by using about 11 microorganism and kenamycin(30µgm/disc)
as standard .
24. The zone of inhibition at different concentration against several
microorganism in compare with standard kenamycin (30
µgm/disc) was shown in given column chart :
0
5
10
15
20
25
30
35
40
45
1000 800 500 Kenamycin (30µgm/disc)
25. In this antimicrobial screening highest inhibitory
activity were noticed against the growth of Bacillus
cereus with the zones of inhibition 30mm at
1000µg/disc. In conclusion, the extract showed
significant zone of inhibition against Bacillus
cereus,Vibro cholera,Shigella dysentery, Eschericia
coli and Salmonella typhi at higher concentration.
26. Based on the present investigation , it can be
assumed that the methanolic extract of Mimosa
invisa (leaves) have antiradical and antimicrobial
activity .Further investigation are required to
isolate the active constituents responsible for the
observed effects and to elucidate the possible
mechanisms of action responsible for those
activities of this plant extract.
