It's a project describing the nutritional composition of aloe vera barbadensis, its antimicrobial properties on selected microbes and the ability of aloe vera to scavenge free radicals.

1. Presented By,
ASHIADEY SELASI KOJO
Supervised by,
DR. NATHANIEL OWUSU BOADI
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2. OUTLINE
 INTRODUCTION
 OBJECTIVES
 METHODOLOGY
 RESULTS AND DISCUSSION
 CONCLUSION
 REFERENCES
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3. INTRODUCTION
Proximate analysis is the quantitative analysis of
food to determine the percentage of fat, fibre, protein,
carbohydrate, moisture and ash. (Merriam Webster
Dictionary)
An antimicrobial is an agent that kills
microorganisms or inhibits their growth. (Merriam
Webster Dictionary)
An antioxidant is a molecule capable of inhibiting
the oxidation of other molecules. (Sies, 1997) Eg
gallic acid and ferulic acid
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4. INTRODUCTION
ALOE VERA
 What is aloe vera?
 Where does it originate from?
 Parts of aloe vera leaf
 Healing properties and general uses
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5. INTRODUCTION
SOME HEALTH BENEFITS OF ALOE VERA
 It is a skin moisturizer
 Lowers high blood pressure
 Lowers high cholesterol
 Stabilizes blood sugar
 Acts as a detoxifier
 Lubricates the digestive tract
(Mike Adams, 2007)
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6. AIMS AND OBJECTIVES
 To estimate the determination of fat, fibre, protein,
moisture, carbohydrate and ash in aloe vera.
 To determine the antimicrobial effect of aloe vera
extract using the agar well diffusion method.
 To determine the antioxidant activity of aloe vera
extract using the DPPH free radical scavenging
method.
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7. METHODOLOGY
EXTRACTION OF THE ALOE VERA GEL
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1 2 3
4
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8. METHODOLOGY
Proximate analysis of aloe vera
 MOISTURE
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%MOISTURE by
oven drying method
%PROTEIN by
Kjeldahl method
%N x 6.25
DIGESTION
using H2SO4
DISTILLATION
of mixture with
CONC NaOH
TITRATION
with 0.1N HCl%LIPIDS by soxhlet
method
%FIBRE by acid and
alkaline digestion of
sample with 1.25%
H2SO4 and 1.25%
NaOH
%ASH by dry
ashing method
%CARBOHYDRATE
by 100 - % ash,
%fibre, %protein and
% fat

9. METHODOLOGY
AGAR WELL DIFFUSION METHOD
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INJECT
BACTERIA
INTO AGAR
PLATE, PURE
NUTRIENT
AGAR
CREAT WELLS IN
AGAR
PIPETTE
EXTRACT IN
WELLS +
CONTROL
INCUBATION

10. METHODOLOGY
ANTIOXIDANT ACTIVITY OF ALOE VERA
 8 serial dilutions of the extract and ascorbic acid(control)
each were prepared
 2ml of DPPH reagent was added to each of the serial
dilutions
 They were incubated in the dark for 30 minutes
 Absorbance was measured at 517nm
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11. Results for proximate analysis
NUTRIENTS RESULTS(Amount of nutrients in %)
Carbohydrate 0.68%
Moisture 99.2%
Ash 0.02%
Fibre 0.06%
Fat 0.01%
Protein 0.027%
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12. RESULTS FOR ANTIMICROBIALANALYSIS
(ZONE OF INHIBITIONS IN MM)
Organisms 20mg/ml 15mg/ml 13mg/ml 10mg/ml
Staphylococcus
aureus
- - - -
Streptococcus
pyogenes
- - - -
Escherichia
Coli
19mm - - -
Pseudomonas
aeroginosa
- - - -
Candida
albicans
4mm - - -
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13. RESULTS FOR ANTIOXIDANT ACTIVITY
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0
10
20
30
40
50
60
70
0.08 0.04 0.02 0.01 0.005 0.002 0.001 0.0006
%inhibition
Concentration in mg/ml
A bar chart of % inhibition against the
concentration of the extract in mg/ml

14. CONCLUSION
 The Proximate analysis of aloe vera gel shows a high level
of carbohydrate and moisture with a little bit of protein,
mineral content and fat. Therefore it can be suitable for
human consumption.
 Aloe vera has antimicrobial activity and can be used to
heal some diseases.
 Aloe vera has antioxidant activity and can be essential to
human health.
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15. REFERENCES
 Mohammad, A. (2003). Aloe Vera. Textbook of
Pharmacognosy. 111-115.
 Atherton, P. (1998), ‘Aloe vera: magic or medicine?’, Nursing
Standard, July 1- 7, 41, 12.
 Park Y, Lee S(2006). New Perspectives on Aloe. New York:
Springer Verlag
 Payne, J.M. (1970). Tissue response to Aloe vera gel following
periodontal surgery. Master's thesis. (accessed October 5, 2009)
 Adams, M. (2005). The aloe vera miracle: A natural medicine
for cancer, cholesterol, diabetes, inflammation, IBS and other
health conditions.
 Sies, H. (1997). "Oxidative stress: Oxidants and antioxidants".
Experimental physiology 82 (2): 291–5
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16. THANK
YOU
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