Bacterial cell inclusions

1. BACTERIAL CELL
INCLUSIONS
PRESENTED BY :
A.DHEERAJ (BP211504),
DEPARTMENT OF MICROBIOLOGY,
SACRED HEART COLLEGE (AUTONOMOUS),
TIRUPATTUR.

2. CELL
INCLUSIONS
 Also known as Inclusions or Cell Inclusions or Inclusion bodies.
 Within the cytoplasm of prokaryotic cells, several kinds of reserve
deposits, known as Inclusions or Cell Inclusions
 It is the storage materials of energy, carbon, phosphorous and
other substances.
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3. TYPES OF CELL
INCLUSIONS
.
1. Metachromatic
Granules
.
3. Lipid 4. Sulphur
inclusions granules .
5.
Carboxysome
s
.
2. Polysaccharide
Granules
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Annual Review
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4. 1.Metachromatic granules
 Metachromatic granules are large inclusions that take their name from the fact that they
sometimes stain red with certain blue dyes such as Methylene blue (Metachromasia).
 Metachromatic Granules are made up of Polymetaphosphates and also called as Volutins or
Volutin Granules or Babe Ernst Granules or Polar Bodies.
 Volutin represents a reserve of Inorganic phosphate (polyphosphate) that can be used in
the synthesis of ATP. It is generally formed by cells that grow in phosphate - rich
environments.
 Metachromatic granules are present in Bacteria (Corynebacterium diphtheriae –
Causative agent of “Diphtheria”), Algae, Fungi and Protozoa.
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5. b) Polysaccharide Granules c) Lipid inclusions
 Polysaccharide granules typically
consist of Glycogen and Starch.
 In the presence of Iodine, Glycogen
granules appears in Red and Starch
granules appears in Blue.
• Lipid inclusions appear in various species
of Mycobacterium, Bacillus, Azotobacter,
Spirillum and other genera.
• A common lipid-storage material, one
unique to bacteria, is the polymer PHB
(Poly – β – hydrobybutyric acid).
• Lipid inclusions are revealed by staining
cells with fat-soluble dyes, such as Sudan
dyes
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Annual Review
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6. d) Sulfur Granules e) Carboxysomes
 Certain bacteria - the "Sulfur bacteria" that
belong to the genus Thiobacillus sp. derive
energy by oxidizing sulfur and sulfur-
containing compounds.
 The Sulfur bacteria may deposit sulfur
granules in the cell, where they serve as an
energy reserve.
 Carboxysomes are the bacterial Cell
inclusions that contain the enzyme
Ribulose 1,5 - diphosphate carboxylase.
 Photosynthetic bacteria use Carbon-di-
oxide as their sole source of carbon and
require this enzyme for Carbon-di-oxide
fixation.
 Carboxysomes containing bacteria are
Nitrifying bacteria, Cyanobacteria and
Thiobacilli.
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Annual Review
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7. Thank you
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