BACTERIAL CELL INCLUSIONS.pptx
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This document presents information about bacterial cell inclusions. It defines cell inclusions as storage materials found within the cytoplasm of prokaryotic cells. The document then describes five main types of cell inclusions: 1) Metachromatic granules which are polyphosphate storage structures that take up dye differently, 2) Polysaccharide granules which store glycogen or starch, 3) Lipid inclusions which store lipids like polyhydroxybutyrate, 4) Sulfur granules found in sulfur-oxidizing bacteria, and 5) Carboxysomes which contain the enzyme RuBisCO for carbon dioxide fixation in photosynthetic bacteria.
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Serum is a complex mix of albumins, growth factors and growth inhibitors.
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Serum from fetal and calf bovine sources are commonly used to support the growth of cells in culture.
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Normal growth media often contain 2-10% of serum.
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Serum provides the basic nutrients (both in the solution as well as bound to the proteins) for cells.
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It also acts a buffer.
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BIOLOGICAL MACROMOLECULES.pptx
This document provides an overview of the four main types of biological macromolecules - carbohydrates, lipids, proteins, and nucleic acids. It defines each macromolecule, describes their monomer units and functions. The key points are that carbohydrates function to store energy, lipids function for long-term energy storage and as cell membrane material, proteins have a variety of functions like structure and enzymes, and nucleic acids involve genetic material DNA and RNA which contain instructions for protein production. The document also provides assessment questions to test understanding of each macromolecule.
BIOLOGICAL MACROMOLECULES.pptx
This document provides an overview of the four main types of biological macromolecules - carbohydrates, lipids, proteins, and nucleic acids. It defines each macromolecule, describes their monomer units and functions. The key points are that carbohydrates function to store energy, lipids function for long-term energy storage and as cell membrane material, proteins have a variety of functions like structure and enzymes, and nucleic acids involve genetic material DNA and RNA which contain instructions for protein production. The document also provides assessment questions to test understanding of each macromolecule.
Organic Compounds
The document discusses the four main types of organic compounds: lipids, proteins, carbohydrates, and nucleic acids. It defines each type, their compositions, functions, and examples. Lipids are made up of carbon, hydrogen, and oxygen and include fats, oils, and waxes. They store and release energy. Proteins contain carbon, hydrogen, oxygen, and nitrogen, and perform essential functions in the body like structure, movement, and immunity. Carbohydrates provide energy and are made of carbon, hydrogen, and oxygen in monosaccharides, disaccharides and polysaccharides like glucose, starch and cellulose. Nucleic acids contain carbon, hydrogen, oxygen, nitrogen and phosphorus and include DNA and RNA
In
This document discusses lipids, which are biomolecules that are soluble in nonpolar solvents. It defines different classes of lipids and describes their biological functions. Specifically, it notes that lipids store energy, act as signaling molecules, and make up cell membranes. It then goes on to describe the different classes of lipids in more detail, including fatty acids, glycerolipids, glycerophospholipids, sphingolipids, sterols, prenols, and others. It also discusses how lipids form bilayers and membranes in aqueous environments due to their amphiphilic nature.
Biomolecules
Biomolecules include carbohydrates, lipids, proteins and nucleic acids. Carbohydrates include monosaccharides like glucose that join to form disaccharides like sucrose or polysaccharides like starch for storage. Lipids are insoluble in water, made of fatty acid esters, and serve as energy stores. Proteins are made of amino acid chains and perform important functions like catalysis and transport.
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Carbohydrates metabolism and lipids biosynthesis and oxidation
Carbohydrates metabolism and lipids biosynthesis and oxidation
Lesson 6 Biomolecules of Physical Science Module.pptx
Lesson 6 Biomolecules of Physical Science Module.pptx
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8.Isolation of pure cultures and preservation of cultures.pdf
Isolation of pure culture, its various method.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptx
Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
Compexometric titration/Chelatorphy titration/chelating titration
Classification
Metal ion ion indicators
Masking and demasking reagents
Estimation of Magnisium sulphate
Calcium gluconate
Complexometric Titration/ chelatometry titration/chelating titration, introduction, Types-
1.Direct Titration
2.Back Titration
3.Replacement Titration
4.Indirect Titration
Masking agent, Demasking agents
formation of complex
comparition between masking and demasking agents,
Indicators/Metal ion indicators/ Metallochromic indicators/pM indicators,
Visual Technique,PM indicators (metallochromic), Indicators of pH, Redox Indicators
Instrumental Techniques-Photometry
Potentiometry
Miscellaneous methods.
Complex titration with EDTA.
Immersive Learning That Works: Research Grounding and Paths Forward
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Phenomics assisted breeding in crop improvement
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
Applied Science: Thermodynamics, Laws & Methodology.pdf
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
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Bob Reedy - Nitrate in Texas Groundwater.pdf
Presented at June 6-7 Texas Alliance of Groundwater Districts Business Meeting
Randomised Optimisation Algorithms in DAPHNE
Slides from talk:
Aleš Zamuda: Randomised Optimisation Algorithms in DAPHNE .
Austrian-Slovenian HPC Meeting 2024 – ASHPC24, Seeblickhotel Grundlsee in Austria, 10–13 June 2024
https://ashpc.eu/
ESA/ACT Science Coffee: Diego Blas - Gravitational wave detection with orbita...
ESA/ACT Science Coffee: Diego Blas - Gravitational wave detection with orbita...Advanced-Concepts-Team
Presentation in the Science Coffee of the Advanced Concepts Team of the European Space Agency on the 07.06.2024.
Speaker: Diego Blas (IFAE/ICREA)
Title: Gravitational wave detection with orbital motion of Moon and artificial
Abstract:
In this talk I will describe some recent ideas to find gravitational waves from supermassive black holes or of primordial origin by studying their secular effect on the orbital motion of the Moon or satellites that are laser ranged.20240520 Planning a Circuit Simulator in JavaScript.pptx
Evaporation step counter work. I have done a physical experiment.
(Work in progress.)
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
快速办理(UAM毕业证书)马德里自治大学毕业证学位证一模一样
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◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
The cost of acquiring information by natural selection
This is a short talk that I gave at the Banff International Research Station workshop on Modeling and Theory in Population Biology. The idea is to try to understand how the burden of natural selection relates to the amount of information that selection puts into the genome.
It's based on the first part of this research paper:
The cost of information acquisition by natural selection
Ryan Seamus McGee, Olivia Kosterlitz, Artem Kaznatcheev, Benjamin Kerr, Carl T. Bergstrom
bioRxiv 2022.07.02.498577; doi: https://doi.org/10.1101/2022.07.02.498577
Recently uploaded (20)
8.Isolation of pure cultures and preservation of cultures.pdf
8.Isolation of pure cultures and preservation of cultures.pdf
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptx
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptx
Compexometric titration/Chelatorphy titration/chelating titration
Compexometric titration/Chelatorphy titration/chelating titration
Immersive Learning That Works: Research Grounding and Paths Forward
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EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
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Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
Applied Science: Thermodynamics, Laws & Methodology.pdf
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GBSN - Biochemistry (Unit 6) Chemistry of Proteins
GBSN - Biochemistry (Unit 6) Chemistry of Proteins
The cost of acquiring information by natural selection
The cost of acquiring information by natural selection
BACTERIAL CELL INCLUSIONS.pptx
- 1. BACTERIAL CELL INCLUSIONS PRESENTED BY : A.DHEERAJ (BP211504), DEPARTMENT OF MICROBIOLOGY, SACRED HEART COLLEGE (AUTONOMOUS), TIRUPATTUR.
- 2. CELL INCLUSIONS Also known as Inclusions or Cell Inclusions or Inclusion bodies. Within the cytoplasm of prokaryotic cells, several kinds of reserve deposits, known as Inclusions or Cell Inclusions It is the storage materials of energy, carbon, phosphorous and other substances. 2 Cell inclusions March 29, 2022
- 3. TYPES OF CELL INCLUSIONS . 1. Metachromatic Granules . 3. Lipid 4. Sulphur inclusions granules . 5. Carboxysome s . 2. Polysaccharide Granules March 29, 2022 Annual Review 3
- 4. 1.Metachromatic granules Metachromatic granules are large inclusions that take their name from the fact that they sometimes stain red with certain blue dyes such as Methylene blue (Metachromasia). Metachromatic Granules are made up of Polymetaphosphates and also called as Volutins or Volutin Granules or Babe Ernst Granules or Polar Bodies. Volutin represents a reserve of Inorganic phosphate (polyphosphate) that can be used in the synthesis of ATP. It is generally formed by cells that grow in phosphate - rich environments. Metachromatic granules are present in Bacteria (Corynebacterium diphtheriae – Causative agent of “Diphtheria”), Algae, Fungi and Protozoa. . . . 4 Cell inclusions March 29, 2022
- 5. b) Polysaccharide Granules c) Lipid inclusions Polysaccharide granules typically consist of Glycogen and Starch. In the presence of Iodine, Glycogen granules appears in Red and Starch granules appears in Blue. • Lipid inclusions appear in various species of Mycobacterium, Bacillus, Azotobacter, Spirillum and other genera. • A common lipid-storage material, one unique to bacteria, is the polymer PHB (Poly – β – hydrobybutyric acid). • Lipid inclusions are revealed by staining cells with fat-soluble dyes, such as Sudan dyes . March 29, 2022 Annual Review 5
- 6. d) Sulfur Granules e) Carboxysomes Certain bacteria - the "Sulfur bacteria" that belong to the genus Thiobacillus sp. derive energy by oxidizing sulfur and sulfur- containing compounds. The Sulfur bacteria may deposit sulfur granules in the cell, where they serve as an energy reserve. Carboxysomes are the bacterial Cell inclusions that contain the enzyme Ribulose 1,5 - diphosphate carboxylase. Photosynthetic bacteria use Carbon-di- oxide as their sole source of carbon and require this enzyme for Carbon-di-oxide fixation. Carboxysomes containing bacteria are Nitrifying bacteria, Cyanobacteria and Thiobacilli. . • . March 29, 2022 Annual Review 6