DNA Polymerase And RNA Polymerase

1. DNA Polymerase
And RNA
Polymerase

2. INTRODUCTION
● What is DNA and RNA Polymerase?
DNA Polymerase for the DNA replication
RNA Polymerase for the transcription
● Arthur Kornberg.

3. DNA POLYMERASE
❏ DNA Polymerase is the prime enzyme that is required for the DNA
replication process.
❏ DNA polymerase also have the different units.
❏ DNA Polymerase can polymerize from 5 prime to 3 prime and
direction of exonuclease activity from 5 prime to 3 prime or 3 prime to 5
prime.

4. STRUCTURE
It is made up of sub-domains resembling an open right hand
as palm, fingers, and thumb. Each of them has its own
function like
❏ The palm contains the catalytic active sites.
❏ The fingers play a major role in nucleotide recognition
and binding.
❏ The thumb is for binding of the DNA substrate.

5. RNA POLYMERASE
STRUCTURE
❏ Two identical α subunits, The C-terminal domain of the
RNA polymerase α subunit plays a key role in molecular
communications.
❏ Similar but not identical β1 and β2 subunits.

6. DIFFERENCE BETWEEN DNA AND RNA POLYMERASE
❏ Helices Requirement For Separate the strands
❏ Nucleotide Requirement To Start The Process
❏ Versatile