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PROKARYOTS DNA
REPLICATION
MOLECULAR BIOLOGY
• WHAT IS REPLICATION ? :
DNA replication is a process by
which a double- stranded dna
molecule is copied to produce
two identical molecules
INTRODUCTION
2
• PROKARYOTIC REPLICATION:
WHEN REPLICATION PROCESS
HAPPENS WITH THE CIRCULAR
DNA OF
PROKAROTIC(BACTERIAL CELL),
THEN IT IS CALLED
PROKARYOTIC REPLICATION
STEPS OF PROKARYOTIC DNAREPLICATION
* According to a Survey
3
STARTING OF
REPLICATION
ELONGATION
FORMATION OF
REPLICATION
FORK
TERMINATION
SEPARATION OF
PARENTAL AND
DAUGHTER DNA
PROKARYOTIC REPLICATION
INITIATION
ENZYMES
4
ENZYMES NEEDED
FOR PROKARYITIC
REPLICATION
DNA A
DNA B
DNA C
SSB
DNA G (PRINASE)
DNA polymerase III
RNAace H
Ligase
TUS protein
INITIATION
5
6
1 2
2 3
ELONGATION
REPLICATION FORK
7
8
TERMINATION
9
10
SIGNIFICANCE OF PROKARYOTIC DNA REPLICATION
Prokaryotic Replication
This process occurs in prokaryotic cells.
It is a continuous process.
Circular, double-stranded DNA
The DNA replicates in the cytoplasm
Single origin of replication
Small amount of DNA
DNA polymerase I and III are involved
Large okazaki fragments
The process is rapid, 2000 base pairs per second
Two circular chromosomes are obtained
DNA gyrase is required
THANK
YOU

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PROKARYOTS DNA REPLICATION.pptx

  • 2. • WHAT IS REPLICATION ? : DNA replication is a process by which a double- stranded dna molecule is copied to produce two identical molecules INTRODUCTION 2 • PROKARYOTIC REPLICATION: WHEN REPLICATION PROCESS HAPPENS WITH THE CIRCULAR DNA OF PROKAROTIC(BACTERIAL CELL), THEN IT IS CALLED PROKARYOTIC REPLICATION
  • 3. STEPS OF PROKARYOTIC DNAREPLICATION * According to a Survey 3 STARTING OF REPLICATION ELONGATION FORMATION OF REPLICATION FORK TERMINATION SEPARATION OF PARENTAL AND DAUGHTER DNA PROKARYOTIC REPLICATION INITIATION
  • 4. ENZYMES 4 ENZYMES NEEDED FOR PROKARYITIC REPLICATION DNA A DNA B DNA C SSB DNA G (PRINASE) DNA polymerase III RNAace H Ligase TUS protein
  • 8. 8
  • 10. 10 SIGNIFICANCE OF PROKARYOTIC DNA REPLICATION Prokaryotic Replication This process occurs in prokaryotic cells. It is a continuous process. Circular, double-stranded DNA The DNA replicates in the cytoplasm Single origin of replication Small amount of DNA DNA polymerase I and III are involved Large okazaki fragments The process is rapid, 2000 base pairs per second Two circular chromosomes are obtained DNA gyrase is required