This document summarizes prokaryotic DNA replication. It describes prokaryotic replication as the process by which a circular, double-stranded DNA molecule in prokaryotic cells is copied to produce two identical molecules. The key steps are initiation at a single origin of replication, elongation of DNA strands by DNA polymerases, and termination resulting in two circular chromosomes. The replication occurs rapidly in the cytoplasm and requires enzymes including DNA polymerases I and III.

1. PROKARYOTS DNA
REPLICATION
MOLECULAR BIOLOGY

2. • WHAT IS REPLICATION ? :
DNA replication is a process by
which a double- stranded dna
molecule is copied to produce
two identical molecules
INTRODUCTION
2
• PROKARYOTIC REPLICATION:
WHEN REPLICATION PROCESS
HAPPENS WITH THE CIRCULAR
DNA OF
PROKAROTIC(BACTERIAL CELL),
THEN IT IS CALLED
PROKARYOTIC REPLICATION

3. STEPS OF PROKARYOTIC DNAREPLICATION
* According to a Survey
3
STARTING OF
REPLICATION
ELONGATION
FORMATION OF
REPLICATION
FORK
TERMINATION
SEPARATION OF
PARENTAL AND
DAUGHTER DNA
PROKARYOTIC REPLICATION
INITIATION

4. ENZYMES
4
ENZYMES NEEDED
FOR PROKARYITIC
REPLICATION
DNA A
DNA B
DNA C
SSB
DNA G (PRINASE)
DNA polymerase III
RNAace H
Ligase
TUS protein

5. INITIATION
5

6. 6
1 2
2 3

7. ELONGATION
REPLICATION FORK
7

8. 8

9. TERMINATION
9

10. 10
SIGNIFICANCE OF PROKARYOTIC DNA REPLICATION
Prokaryotic Replication
This process occurs in prokaryotic cells.
It is a continuous process.
Circular, double-stranded DNA
The DNA replicates in the cytoplasm
Single origin of replication
Small amount of DNA
DNA polymerase I and III are involved
Large okazaki fragments
The process is rapid, 2000 base pairs per second
Two circular chromosomes are obtained
DNA gyrase is required

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