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BIOFERTILLIZERS.GROUPS OF BIOFERTILLIZERS.SCOPE AND FUTURE
PERSPECTIVE OF BIOFERTILLIZERS
IRAM SHAFIQ
13-ARID-320
SEMESTER : 7th AGRONOMY
BIOLOGICAL NITROGEN
FIXATION
AGR-603
HAFSA NAZIR CHEEMA
13-ARID-320
PRESENTED BY :
PRESENTED TO: DR RASHEED
1
CONTENTS
INTRODUCTION
1
2
DIFFERENT GROUPS OF BIOFERTILIZERS
3
APPLICATION OF BIOFERTILIZERS TO CROPS
4
CONSTRAINTS FOR ADAPTATION OF BIOFERTILIZER
TECHNOLOGY
5
WHY DO WE NEED BIOFERTILLIZERS?
6
SCOPE AND FUTURE PERSPECTIVES OF
BIOFERTILIZERS
2
BIOFERTILIZERS
• A substance which contains living
microorganisms ,which, when applied to
seed , plant surfaces , or soil ,colonizes the
rhizosphere or interior of the plant and
promotes growth by increasing the supply or
availability of primary nutrients to plants .
• Add nutrients through the natural processes
of nitrogen fixation , solubilizing
phosphorous and stimulating plant growth
through the synthesis of growth –stimulating
substances.
• Also named as microbial inoculants .
• Ready to use live formulation of beneficial
microorganisms for enhancing the
productivity of soil.
3
LOGO
• Its eco friendly in that it not only prevents,
damaging the natural resource but also helps
to some extent cleanse the plant from
precipitated chemical fertilizers which have
damaging effects like;
• Polluting water basins.
• Destroying microorganisms and beneficial
insects.
• Reducing soil fertility and irreparable damage
• Bio-fertilizer is technically living , it can
symbiotically associate with plant roots.
Involved microorganisms can readily
and safely convert complex organic
material in simple compounds ,so that
plants can easily take up.
• Maintains stomatal functioning turgor
potential and transpiration.
• It maintains the natural habitat of the
soil
• It increases crop yield by 20-30%.
• Biofertilizers provide protection against
drought and some soil-borne diseases.
• Fortify the soil and improves soil
structure and profile
• Replaces chemical nitrogen and
phosphorous by 25%,and stimulates
plant growth.
• Bio-fertilizers are cost effective relative
to chemical fertilizer.
WHY DO WE NEED BIOFERTILIZERS
4
They scavenge
phosphate from
soil layers .
Solubilise the insoluble
form of phosphates like
tricalcium,iron and
aluminum phosphate into
available forms.
Fix atmospheric N in the soil
and root nodules of legume
crops and make it available to
the plants.
HOW DOES BIOFERTILIZER WORKS
Promotes hormones and
metabolites which promotes
root growth .
Decompose organic
matter and help in soil
mineralization.
5
CLASSIFICATION OF BIOFERTILIZERS
Bacteria.
Bacillus subtilis,
Pseudomonas striata
Fungi.
Pencillium sp,
Aspergillus awamori
P solubilizing
Biofertilizer
•Arbuscular
mycorrizha.
•Glomus sp
• Ectomycorrizha.
•Amanita sp
•Ericoid mycorr…
•Pezizella ericae
P mobilizing
biofertilizer
• silicate and zinc
Solubilizers.
•Bacillus sp
Biofertilizers for
Micro-nutrients
• Free living.
•Azotobactor,
Anabeana,noctoc
• Symbiotic .
Rhizobium,azollae
• Associative .
•Azospirillum
Nitrogen fixing
biofertilizer
BIOFERTILIZERS
6
.
Plant growth
promoting
Rhizobacteria
Pseudomonas .
• Pseudomonas sp
LOGO
AZOTOBACTER
 Found in neutral to
alkaline soils.
 Azotobacter belongs to
azotobacteriaceae.
Produce polysaccrides
which improves soil
aggregation.
 Fix biologically active
PGRs like IAA and
gibberellins.
Anabaena azollae
Free- floating water fern
used as biofertilizer for
wetland rice.
Fixes atmospheric N in
association with N fixing
PGA (Anabaena azollae
known to contribute
40-60 Kg N/ha per rice
crop.
RHIZOBIUM
Soil habitat bacterium
able to colonize the
legume roots.
 Belongs to rhizobiaceae.
Fixes 50-100 kg /ha/yr
of nitrogen.
Fixes atmospheric
elemental N symbiotically
into plant useable form.
AZOSPIRILLIUM
Proliferates under both
anaerobic and aerobic
conditions.
Belongs to spirillaceae
family.
Nitrogen fixing ability of
20-40 kg/ha.
PGRs production(IAA),
disease resistance and
drought tolerance are
other benefits.
Nitrogen fixing biofertilizer
FREE LIVING SYMBIOTIC ASSOCIATIVE
7
LOGO
APPLICATION OF BIOFERTILLIZER
SEED TREATMENT
(Seed inoculation method)
 The inoculant (bacteria-carrier mixture)
is mixed with water to make slurry and
then mixed with seeds.
SEEDLING ROOT DIP
(Root inoculation method)
For vegetables mix both N and
P bio fertilizers in water.
Dip the roots of seedlings in
this suspension for 30-40 min
before transplanting.
 For paddy make a bed in the
field and fill it with water.
 Mix bio fertilizers in water and
dip the roots of seedlings for 8-
10 hrs.
SOIL TREATMENT
(Soil inoculation)
 All biofertilizers along with the
compost fertilizers are mixed together.
They are kept for one night .
Then the next day this mixture is
spread on the soil where seeds have to
be sown.
For inoculation in general, granular
inoculant is placed into the furrow under
or alongside the seed.
This enhances the chance for
inoculated strain to be in contact with
plant roots.
The slurry is then poured over the
seeds spread on a cemented floor and
mixed properly in a way that a thin layer
is formed around the seeds.
 The treated seeds should be dried in
the shade overnight and then they
should be used.
Generally, 750 gram of biofertilizer is
required to treat the legume seeds for
one hectare area
8
LOGO
PHOSPHATE SOLUBILIZING BIOFERTILIZER
S W
OT
 Group of beneficial bacteria capable of
hydrolyzing organic and inorganic
phosphorous from insoluble compounds.
 Releases insoluble phosphorus in soil and fix
in clay minerals.
 Example includes pseudomonas, species of
bacillus ,penicillium and Aspergillus .
 Seed inoculation of PSB-30 kg of
phosphorous /ha.
9
PHOSPHATE MOBILIZING
BIOFERTILIZER(mycorrizha)
 A symbiotic ,generally mutualistic association between a
fungus and roots of vascular plants.
 Transfer P from the soil to the root cortex.
 The fungus colonizes the host plant roots, either
interacellularly or extracellularly and increase surface area
of roots.
 This association provides the fungus with access to
carbohydrates.
 In return ,the plant gains the benefits of mycelium higher
absorptive capacity for water and mineral.
 Plants roots alone may be incapable of taking up phosphate
ions that are demineralized in soil with basic PH.
 The mycelium of the mycorrhizal fungus can make P
available to the plants they colonize.
10
BIOFERTILIZERS
SILICATE AND ZINC SOLUBILIZING
BIOFERTILIZER
Micro-organisms are capable of degrading
silicates and aluminum silicates.
 Bacterial sp degrade silicates and aluminum
silicates in soil.
Help in silicate weathering.
E.g. Bacillus sp can be used as bio-fertilizer
for zinc or aluminum silicates because these
organisms solubilize the zinc present in the
soil and make it available to the plants.
PLANT GROWTH PROMOTING
RHIZOBACTERIA
Act s both biofertilizer and biopesticide.
Promote growth by:
Improved Nutrient Availability (Biofertilizers)
Suppression of Plant Disease (Bioprotectants)
 Phytohormones Production (Biostimulants)
The produce include indole-acetice acid
,cytokinins,gibberellins and inhibitors of
ethylene production.
11
LOGO
CONSTRAINTS FOR ADOPTION OF BIOFERTILIZER
TECHNOLOGY
Specific to plants.eg
Rhizobium sp culture
does᾿t work well in high
nitrate tolerant strains.
Short shelf life.
Requires qualified
technical persons.
 Prone to
contamination.
The acceptability of
biofertilizers has been rather
low because they do not
produce quick and
spectacular response.
Lack of regulation and
standards, quality issues of the
product.
High initial investment to
production unit.
lack of publicity are the major
constraints in the widespread
use of biofertilizers in Pakistan.
As such there is no harmful impacts of biofertilizers if used
properly. However some constraints are:
LOGO12
LOGO
SCOPE AND FUTURE PERSPECTIVES OF
BIOFERTILIZERS
The use of biofertilizers
can play an important role
in sustaining the
agriculture systems. In
Pakistan, where fertilizer
is annually a hundred
billion rupees business,
even a 10% contribution
by biofertilizer can save
rupees 10 billion.
Biofertilizers offer a huge
potential for widespread use
offering both economical and
environmental advantage to
farmers/growers and
commercial viability to
production units.
Various research
groups/organizations are
engaged in research and
development on
biofertilizers and have
made their efforts to
increase the application of
biofertilizers in Pakistan's
agriculture
Assuring quality of
products, concept of food
security, and concept of
safe food with extensive
field-based testing, capacity
building of resource persons
and stakeholders on
standard production
processes, storage and
application will help a wider
adoption and
popularization of the
biofertilizer technologies in
Pakistan
13
LOGO
Thank
you
REFERENCES
•https://books.google.com.
•https://en.wikipedia.org.
• pakagrifarming.blogspot.com/2013/03/introduction-to-biofertilizers.html.
• https://agroproducts.wordpress.com/tag/advantages-of-bio-fertilizers.
• www.biotecharticles.com/.../Biofertilizers-Types-Benefits-and-Applications

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BIOFERTILLIZERS.GROUPS OF BIOFERTILLIZERS.SCOPE AND FUTURE PERSPECTIVE OF BIOFERTILLIZERS

  • 1. BIOFERTILLIZERS.GROUPS OF BIOFERTILLIZERS.SCOPE AND FUTURE PERSPECTIVE OF BIOFERTILLIZERS IRAM SHAFIQ 13-ARID-320 SEMESTER : 7th AGRONOMY BIOLOGICAL NITROGEN FIXATION AGR-603 HAFSA NAZIR CHEEMA 13-ARID-320 PRESENTED BY : PRESENTED TO: DR RASHEED 1
  • 2. CONTENTS INTRODUCTION 1 2 DIFFERENT GROUPS OF BIOFERTILIZERS 3 APPLICATION OF BIOFERTILIZERS TO CROPS 4 CONSTRAINTS FOR ADAPTATION OF BIOFERTILIZER TECHNOLOGY 5 WHY DO WE NEED BIOFERTILLIZERS? 6 SCOPE AND FUTURE PERSPECTIVES OF BIOFERTILIZERS 2
  • 3. BIOFERTILIZERS • A substance which contains living microorganisms ,which, when applied to seed , plant surfaces , or soil ,colonizes the rhizosphere or interior of the plant and promotes growth by increasing the supply or availability of primary nutrients to plants . • Add nutrients through the natural processes of nitrogen fixation , solubilizing phosphorous and stimulating plant growth through the synthesis of growth –stimulating substances. • Also named as microbial inoculants . • Ready to use live formulation of beneficial microorganisms for enhancing the productivity of soil. 3
  • 4. LOGO • Its eco friendly in that it not only prevents, damaging the natural resource but also helps to some extent cleanse the plant from precipitated chemical fertilizers which have damaging effects like; • Polluting water basins. • Destroying microorganisms and beneficial insects. • Reducing soil fertility and irreparable damage • Bio-fertilizer is technically living , it can symbiotically associate with plant roots. Involved microorganisms can readily and safely convert complex organic material in simple compounds ,so that plants can easily take up. • Maintains stomatal functioning turgor potential and transpiration. • It maintains the natural habitat of the soil • It increases crop yield by 20-30%. • Biofertilizers provide protection against drought and some soil-borne diseases. • Fortify the soil and improves soil structure and profile • Replaces chemical nitrogen and phosphorous by 25%,and stimulates plant growth. • Bio-fertilizers are cost effective relative to chemical fertilizer. WHY DO WE NEED BIOFERTILIZERS 4
  • 5. They scavenge phosphate from soil layers . Solubilise the insoluble form of phosphates like tricalcium,iron and aluminum phosphate into available forms. Fix atmospheric N in the soil and root nodules of legume crops and make it available to the plants. HOW DOES BIOFERTILIZER WORKS Promotes hormones and metabolites which promotes root growth . Decompose organic matter and help in soil mineralization. 5
  • 6. CLASSIFICATION OF BIOFERTILIZERS Bacteria. Bacillus subtilis, Pseudomonas striata Fungi. Pencillium sp, Aspergillus awamori P solubilizing Biofertilizer •Arbuscular mycorrizha. •Glomus sp • Ectomycorrizha. •Amanita sp •Ericoid mycorr… •Pezizella ericae P mobilizing biofertilizer • silicate and zinc Solubilizers. •Bacillus sp Biofertilizers for Micro-nutrients • Free living. •Azotobactor, Anabeana,noctoc • Symbiotic . Rhizobium,azollae • Associative . •Azospirillum Nitrogen fixing biofertilizer BIOFERTILIZERS 6 . Plant growth promoting Rhizobacteria Pseudomonas . • Pseudomonas sp
  • 7. LOGO AZOTOBACTER  Found in neutral to alkaline soils.  Azotobacter belongs to azotobacteriaceae. Produce polysaccrides which improves soil aggregation.  Fix biologically active PGRs like IAA and gibberellins. Anabaena azollae Free- floating water fern used as biofertilizer for wetland rice. Fixes atmospheric N in association with N fixing PGA (Anabaena azollae known to contribute 40-60 Kg N/ha per rice crop. RHIZOBIUM Soil habitat bacterium able to colonize the legume roots.  Belongs to rhizobiaceae. Fixes 50-100 kg /ha/yr of nitrogen. Fixes atmospheric elemental N symbiotically into plant useable form. AZOSPIRILLIUM Proliferates under both anaerobic and aerobic conditions. Belongs to spirillaceae family. Nitrogen fixing ability of 20-40 kg/ha. PGRs production(IAA), disease resistance and drought tolerance are other benefits. Nitrogen fixing biofertilizer FREE LIVING SYMBIOTIC ASSOCIATIVE 7
  • 8. LOGO APPLICATION OF BIOFERTILLIZER SEED TREATMENT (Seed inoculation method)  The inoculant (bacteria-carrier mixture) is mixed with water to make slurry and then mixed with seeds. SEEDLING ROOT DIP (Root inoculation method) For vegetables mix both N and P bio fertilizers in water. Dip the roots of seedlings in this suspension for 30-40 min before transplanting.  For paddy make a bed in the field and fill it with water.  Mix bio fertilizers in water and dip the roots of seedlings for 8- 10 hrs. SOIL TREATMENT (Soil inoculation)  All biofertilizers along with the compost fertilizers are mixed together. They are kept for one night . Then the next day this mixture is spread on the soil where seeds have to be sown. For inoculation in general, granular inoculant is placed into the furrow under or alongside the seed. This enhances the chance for inoculated strain to be in contact with plant roots. The slurry is then poured over the seeds spread on a cemented floor and mixed properly in a way that a thin layer is formed around the seeds.  The treated seeds should be dried in the shade overnight and then they should be used. Generally, 750 gram of biofertilizer is required to treat the legume seeds for one hectare area 8
  • 9. LOGO PHOSPHATE SOLUBILIZING BIOFERTILIZER S W OT  Group of beneficial bacteria capable of hydrolyzing organic and inorganic phosphorous from insoluble compounds.  Releases insoluble phosphorus in soil and fix in clay minerals.  Example includes pseudomonas, species of bacillus ,penicillium and Aspergillus .  Seed inoculation of PSB-30 kg of phosphorous /ha. 9
  • 10. PHOSPHATE MOBILIZING BIOFERTILIZER(mycorrizha)  A symbiotic ,generally mutualistic association between a fungus and roots of vascular plants.  Transfer P from the soil to the root cortex.  The fungus colonizes the host plant roots, either interacellularly or extracellularly and increase surface area of roots.  This association provides the fungus with access to carbohydrates.  In return ,the plant gains the benefits of mycelium higher absorptive capacity for water and mineral.  Plants roots alone may be incapable of taking up phosphate ions that are demineralized in soil with basic PH.  The mycelium of the mycorrhizal fungus can make P available to the plants they colonize. 10
  • 11. BIOFERTILIZERS SILICATE AND ZINC SOLUBILIZING BIOFERTILIZER Micro-organisms are capable of degrading silicates and aluminum silicates.  Bacterial sp degrade silicates and aluminum silicates in soil. Help in silicate weathering. E.g. Bacillus sp can be used as bio-fertilizer for zinc or aluminum silicates because these organisms solubilize the zinc present in the soil and make it available to the plants. PLANT GROWTH PROMOTING RHIZOBACTERIA Act s both biofertilizer and biopesticide. Promote growth by: Improved Nutrient Availability (Biofertilizers) Suppression of Plant Disease (Bioprotectants)  Phytohormones Production (Biostimulants) The produce include indole-acetice acid ,cytokinins,gibberellins and inhibitors of ethylene production. 11
  • 12. LOGO CONSTRAINTS FOR ADOPTION OF BIOFERTILIZER TECHNOLOGY Specific to plants.eg Rhizobium sp culture does᾿t work well in high nitrate tolerant strains. Short shelf life. Requires qualified technical persons.  Prone to contamination. The acceptability of biofertilizers has been rather low because they do not produce quick and spectacular response. Lack of regulation and standards, quality issues of the product. High initial investment to production unit. lack of publicity are the major constraints in the widespread use of biofertilizers in Pakistan. As such there is no harmful impacts of biofertilizers if used properly. However some constraints are: LOGO12
  • 13. LOGO SCOPE AND FUTURE PERSPECTIVES OF BIOFERTILIZERS The use of biofertilizers can play an important role in sustaining the agriculture systems. In Pakistan, where fertilizer is annually a hundred billion rupees business, even a 10% contribution by biofertilizer can save rupees 10 billion. Biofertilizers offer a huge potential for widespread use offering both economical and environmental advantage to farmers/growers and commercial viability to production units. Various research groups/organizations are engaged in research and development on biofertilizers and have made their efforts to increase the application of biofertilizers in Pakistan's agriculture Assuring quality of products, concept of food security, and concept of safe food with extensive field-based testing, capacity building of resource persons and stakeholders on standard production processes, storage and application will help a wider adoption and popularization of the biofertilizer technologies in Pakistan 13

Editor's Notes

  1. Agenda, parts
  2. Keywords: plant, sapling, benefit, investment