To differentiate between the mutated and normal genes, the DNA of the person is isolated and amplified by the PCR (polymerase chain reaction). This genome is subjected to the DNA microarrays, i.e. DNA microchips that contain the single strand sequences of normal genes including the sequences of its possible mutations. The DNA is cut into small fragments and these fragments are labelled with different fluorescent dyes (the sample from the patient and the normal DNA sample). Both the DNA are allowed to hybridize and bind to the single strands of the DNA chip. The difference in the percent hybridization indicates the presence of mutations. if the person does not have any mutations, both the samples hybridize equivalents and produce the same fluorescence. Thus, if the person has the retinoblastoma mutant gene, two different fluorescences will appear, indicating the hybridizations of the mutant and the normal genes whereas an unaffected person produces unifom fluorescence. Solution To differentiate between the mutated and normal genes, the DNA of the person is isolated and amplified by the PCR (polymerase chain reaction). This genome is subjected to the DNA microarrays, i.e. DNA microchips that contain the single strand sequences of normal genes including the sequences of its possible mutations. The DNA is cut into small fragments and these fragments are labelled with different fluorescent dyes (the sample from the patient and the normal DNA sample). Both the DNA are allowed to hybridize and bind to the single strands of the DNA chip. The difference in the percent hybridization indicates the presence of mutations. if the person does not have any mutations, both the samples hybridize equivalents and produce the same fluorescence. Thus, if the person has the retinoblastoma mutant gene, two different fluorescences will appear, indicating the hybridizations of the mutant and the normal genes whereas an unaffected person produces unifom fluorescence..

1. To differentiate between the mutated and normal genes, the DNA of the person is isolated and
amplified by the PCR (polymerase chain reaction). This genome is subjected to the DNA
microarrays, i.e. DNA microchips that contain the single strand sequences of normal genes
including the sequences of its possible mutations.
The DNA is cut into small fragments and these fragments are labelled with different fluorescent
dyes (the sample from the patient and the normal DNA sample). Both the DNA are allowed to
hybridize and bind to the single strands of the DNA chip. The difference in the percent
hybridization indicates the presence of mutations. if the person does not have any mutations,
both the samples hybridize equivalents and produce the same fluorescence.
Thus, if the person has the retinoblastoma mutant gene, two different fluorescences will appear,
indicating the hybridizations of the mutant and the normal genes whereas an unaffected person
produces unifom fluorescence.
Solution
To differentiate between the mutated and normal genes, the DNA of the person is isolated and
amplified by the PCR (polymerase chain reaction). This genome is subjected to the DNA
microarrays, i.e. DNA microchips that contain the single strand sequences of normal genes
including the sequences of its possible mutations.
The DNA is cut into small fragments and these fragments are labelled with different fluorescent
dyes (the sample from the patient and the normal DNA sample). Both the DNA are allowed to
hybridize and bind to the single strands of the DNA chip. The difference in the percent
hybridization indicates the presence of mutations. if the person does not have any mutations,
both the samples hybridize equivalents and produce the same fluorescence.
Thus, if the person has the retinoblastoma mutant gene, two different fluorescences will appear,
indicating the hybridizations of the mutant and the normal genes whereas an unaffected person
produces unifom fluorescence.