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STPM/S(E)964



                             MAJLIS PEPERIKSAAN MALAYSIA
                            (MALAYSIAN EXAMINATIONS COUNCIL)




             PEPERIKSAAN
SIJIL TINGGI PERSEKOLAHAN MALAYSIA
 (MALAYSIA HIGHER SCHOOL CERTIFICATE EXAMINATION)




                               BIOLOGY
Syllabus, Specimen Papers and Specimen Experiment




  This syllabus applies for the 2012/2013 session and thereafter until further notice.
FALSAFAH PENDIDIKAN KEBANGSAAN

“Pendidikan di Malaysia adalah satu usaha berterusan
ke arah memperkembangkan lagi potensi individu secara
menyeluruh dan bersepadu untuk mewujudkan insan yang
seimbang dan harmonis dari segi intelek, rohani, emosi,
dan jasmani. Usaha ini adalah bagi melahirkan rakyat
Malaysia yang berilmu pengetahuan, berakhlak mulia,
bertanggungjawab, berketerampilan, dan berkeupayaan
mencapai kesejahteraan diri serta memberi sumbangan
terhadap keharmonian dan kemakmuran keluarga,
masyarakat dan negara.”
FOREWORD

This revised Biology syllabus is designed to replace the existing syllabus which has been in use since
the 2001 STPM examination. This new syllabus will be enforced in 2012 and the first examination
will also be held the same year. The revision of the syllabus takes into account the changes made by
the Malaysian Examinations Council (MEC) to the existing STPM examination. Through the new
system, sixth-form study will be divided into three terms, and candidates will sit for an examination at
the end of each term. The new syllabus fulfils the requirements of this new system. The main
objective of introducing the new examination system is to enhance the teaching and learning
orientation in sixth form so as to be in line with the orientation of teaching and learning in colleges
and universities.

The revision of the Biology syllabus incorporates current developments in biology studies to be more
relevant to the current global developments. Biology is a science that deals with the study of living
organisms. It is dynamic and affects every aspect of our daily lives, from food and health, to the
responsibilities towards our environment. The cumulative discoveries and developments in biology
have tremendously enhanced our understanding and perception towards both the unity and diversity of
life. With this understanding, we have become more aware of our interdependence with our rich
biodiversity and natural resources. The application of biology together with modern technologies
leads to the evolution of biotechnology. Furthermore, the assessment tools of this syllabus consist of
written papers and coursework. The written papers evaluate candidates’ knowledge and understanding
of the subject, while the coursework provides an opportunity for candidates to trigger their inquisitive
biological reasoning. This also enhances their understanding and application of biological sciences
and develops the candidates’ soft skills.

The syllabus contains topics, teaching periods, learning outcomes, examination format, grade
description, and sample questions.

The design of this syllabus was undertaken by a committee chaired by Professor Emeritus Dato’ Dr.
Latiff bin Mohamad of Universiti Kebangsaan Malaysia. Other committee members consist of
university lecturers, representatives from the Curriculum Development Division, Ministry of
Education Malaysia, and experienced teachers teaching Biology. On behalf of the Malaysian
Examinations Council, I would like to thank the committee for their commitment and invaluable
contribution. It is hoped that this syllabus will be a guide for teachers and candidates in the teaching
and learning process.



OMAR BIN ABU BAKAR
Chief Executive
Malaysian Examinations Council
CONTENTS

                               Syllabus 964 Biology

                                                                       Page

Aims                                                                     1

Objectives                                                               1

Content

    First Term:    Biological Molecules and Metabolism                 2–8

    Second Term: Physiology                                            9 – 15

    Third Term:    Ecology and Genetics                               16 – 21

Practical Syllabus (School-based Assessment of Practical (Paper 4))     22

Written Practical Test (Paper 5)                                        23

Scheme of Assessment                                                  24 – 25

Performance Descriptions                                                26

Reference Books                                                         27

Specimen Paper 1                                                      29 – 43

Specimen Paper 2                                                      45 – 63

Specimen Paper 3                                                      65 – 81

Specimen Experiment Paper 4                                           83 – 86

Specimen Paper 5                                                      87 – 101
SYLLABUS
                                            964 BIOLOGY


Aims

This syllabus is designed to enhance candidates’ knowledge and understanding of biology and
biological issues, to prepare and equip the candidates for their tertiary education, to pursue careers in
related fields and to promote continuous awareness of the importance of biology in life.


Objectives

This syllabus enables candidates to:
(a)    understand the biological phenomena, principles and theories;
(b)    evaluate biological information critically and deduce logical conclusion;
(c)    plan and carry out experiments scientifically and make deductions;
(d)    develop abilities and skills in handling materials and apparatus correctly and safely, and;
(e)    cultivate proper attitudes and values on social, technological, and environmental issues in
       biology.




                                                    1
FIRST TERM: BIOLOGICAL MOLECULES AND METABOLISM

                           Teaching
             Topic                                    Learning Outcome
                            Period

1   Biological Molecules     24

    1.1   Water               2       Candidates should be able to:

                                      (a) describe the chemical properties (solvent, bond
                                          angles and hydrogen bond) of water and relate
                                          its physiological roles in the organisms;
                                      (b) describe the physical properties (polarity,
                                          cohesiveness, density, surface tension, specific
                                          heat capacity, and latent heat of vaporisation)
                                          of water and relate its physiological roles in
                                          organisms.

    1.2   Carbohydrates       6       Candidates should be able to:

                                      (a) classify carbohydrates into monosaccharide,
                                          disaccharide and polysaccharide with respect
                                          to their physical and chemical properties;
                                      (b) classify monosaccharide according to the
                                          number of carbon atoms and the functional
                                          groups
                                            (i) triose e.g. glyceraldehydes,
                                           (ii) pentose e.g. ribose and deoxyribose,
                                           (iii) hexose e.g. glucose and fructose,
                                      (c) illustrate the molecular structure of a
                                          monosaccharide and differentiate between the
                                          reducing and non-reducing ends;
                                      (d) describe the formation of glycosidic bond in
                                          disaccharides (maltose and sucrose) and
                                          polysaccharides (starch, glycogen and
                                          cellulose);
                                      (e) relate the structure of disaccharides and
                                          polysaccharides to their functions in living
                                          organisms.




                                      2
Teaching
         Topic                                     Learning Outcome
                       Period

1.3   Lipids             3       Candidates should be able to:

                                 (a) describe the structures, properties and
                                     distribution of triglycerides, phospholipids
                                     (lecithin) and steroid (cholesterol);
                                 (b) state the functions of triglycerides,
                                     phospholipids (lecithin) and steroids
                                     (cholesterol);
                                 (c) differentiate between saturated and unsaturated
                                     fatty acids.

1.4   Proteins           6       Candidates should be able to:

                                 (a) classify amino acids into four main classes
                                     based on their side chains: polar, non-polar,
                                     acidic and basic;
                                 (b) describe the structure of an amino acid and the
                                     formation of peptide bonds in polypeptides;
                                 (c) explain the properties of protein (amphoteric,
                                     isoelectric point, buffer and colloid);
                                 (d) differentiate the various levels of organisation
                                     of protein structure (primary, secondary,
                                     tertiary and quaternary) and relate the
                                     functions of each structure to the organisation
                                     of proteins;
                                 (e) explain the denaturation and renaturation of
                                     protein;
                                 (f)   classify proteins according to their structures,
                                       compositions (simple and conjugated) and
                                       functions.

1.5   Nucleic acids      3       Candidates should be able to:

                                 (a) describe the structures of nucleotides and the
                                     formation of phosphodiester bonds in a
                                     polynucleotide;
                                 (b) distinguish between DNA and RNA and the
                                     three types of RNAs (mRNA, tRNA and
                                     rRNA);
                                 (c) describe the structure of DNA based on
                                     Watson and Crick model.




                                 3
Teaching
             Topic                                            Learning Outcome
                                   Period

    1.6   Analytical techniques      4       Candidates should be able to:

                                             (a) describe the basic principles of paper
                                                 chromatography in pigment separation,
                                                 electrophoresis for protein and nucleic acid
                                                 separation.

2   Structure of Cells and          16
    Organelles

    2.1   Prokaryotic and            4       Candidates should be able to:
          eukaryotic cells
                                             (a) state the cell theory;
                                             (b) compare the structures of prokaryotic and
                                                 eukaryotic cells;
                                             (c) compare typical animal and plant cells as seen
                                                 under electron microscopes;
                                             (d) describe the basic principles of light and
                                                 electron microscopy.

    2.2   Cellular components        6       Candidates should be able to:

                                             (a) identify the cellular components of typical
                                                 plant and animal cells;
                                             (b) describe the structures of organelles and state
                                                 their functions;
                                             (c) explain the basic principles of differential
                                                 centrifugation used to fractionate cellular
                                                 components (g and S values).

    2.3   Specialised cells          6       Candidates should be able to:

                                             (a) outline the structures, functions and
                                                 distributions of unspecialised cells found in
                                                 plants (meristematic cells);
                                             (b) describe the structures, functions and
                                                 distributions of specialised plant cells found in
                                                 epidermal, ground and vascular tissue;
                                             (c) describe the structures, functions and
                                                 distributions of specialised animal cells found
                                                 in connective, nervous, muscular and epithelial
                                                 tissues, including the formation of endocrine
                                                 and exocrine glands.




                                             4
Teaching
              Topic                                         Learning Outcome
                                 Period

3   Membrane Structure and         8
    Transport

    3.1   Fluid mosaic model       3       Candidates should be able to:
                                           (a) describe the structure of a membrane based on
                                               Singer-Nicolson fluid mosaic model;
                                           (b) explain the roles of each component of the
                                               membrane.

    3.2   Movement of              5       Candidates should be able to:
          substance across
          membrane                         (a) explain the processes of passive and active
                                               transports, endocytosis and exocytosis;
                                           (b) explain the concepts of water potential, solute
                                               potential and pressure potential;
                                           (c) calculate the water potential of a plant cell in a
                                               solution.

4   Enzymes                       20

    4.1   Catalysis and            3       Candidates should be able to:
          activation energy
                                           (a) explain that enzyme is a globular protein
                                               which catalyses a metabolic reaction;
                                           (b) explain the mode of action of enzymes at
                                               active site involving enzyme-substrate
                                               complex and lowering of the activation energy
                                               and enzyme specificity.

    4.2   Mechanism of action      5       Candidates should be able to:
          and kinetics
                                           (a) illustrate enzyme specificity using induced fit
                                               (Koshland) and lock and key (Fischer) models;
                                           (b) explain the time course of an enzyme-
                                               catalysed reaction by measuring the rate of
                                               formation of product(s) or rate of
                                               disappearance of substrate(s) as the rate of
                                               reaction;
                                           (c) deduce the Michaelis-Menten constant (Km)
                                               from the Michaelis-Menten and Lineweaver-
                                               Burk plots;
                                           (d) explain the significance of Km and Vmax;
                                           (e) explain the effects of temperature, pH, enzyme
                                               concentration and substrate concentration on
                                               the rate of an enzyme-catalysed reaction.




                                           5
Teaching
             Topic                                             Learning Outcome
                                    Period

    4.3   Cofactors                   3       Candidates should be able to:

                                              (a) explain the roles of cofactors (ion activators,
                                                  coenzymes and prosthetic groups) in an
                                                  enzymatic reaction;
                                              (b) explain the importance of vitamins and
                                                  minerals as precursors of
                                                  coenzymes/cofactors.

    4.4   Inhibitors                  4       Candidates should be able to:

                                              (a) explain the effects of competitive and non-
                                                  competitive inhibitions on the rate of enzyme
                                                  activity of reversible inhibition;
                                              (b) relate the Lineweaver-Burk plot to the effect of
                                                  inhibition on Km and Vmax values.

    4.5   Classification of           2       Candidates should be able to:
          enzymes
                                              (a) describe enzyme classification according to
                                                  International Union of Biochemistry (IUB)
                                                  e.g. oxidoreductase, transferase, hydrolase,
                                                  lyase, isomerase and ligase.

    4.6   Enzyme technology           3       Candidates should be able to:

                                              (a) explain the importance and the main
                                                  techniques of enzyme immobilisation namely
                                                  adsorption, entrapment and covalent coupling;
                                              (b) explain the application of enzyme
                                                  immobilisation in the development of
                                                  biosensors.

5   Cellular Respiration             12

    5.1   The need for energy in      1       Candidates should be able to:
          living
                                              (a) outline the importance of energy and
                                                  respiration in living organisms;
                                              (b) describe the structure of the energy carriers
                                                  such as ATP, NADH and FADH2.

    5.2   Aerobic respiration         8       Candidates should be able to:

                                              (a) describe the various stages of aerobic
                                                  respiration and its location in the cells;
                                              (b) describe glycolysis, and calculate the net
                                                  energy produced in glycolysis;




                                              6
Teaching
             Topic                                             Learning Outcome
                                   Period

                                             (c) describe the various steps involved in the
                                                 Krebs cycle (including the link reaction);
                                             (d) explain the formation of NADH, FADH2, GTP
                                                 and ATP during the Krebs cycle;
                                             (e) describe oxidative phosphorylation and
                                                 chemiosmosis in the electron transport system;
                                             (f)   explain the role of NADH, FADH2 and ATP
                                                   synthase in the electron transport chain;
                                             (g) calculate and explain the net energy produced
                                                 in aerobic respiration per molecule of glucose
                                                 in liver and muscle cells;
                                             (h) describe the effects of cyanide and carbon
                                                 monoxide on respiration;
                                             (i)   explain how lipid and protein act as alternative
                                                   energy sources.

    5.3   Anaerobic respiration      3       Candidates should be able to:

                                             (a) explain the anaerobic respiration in yeast and
                                                 muscle cells;
                                             (b) describe the applications of anaerobic
                                                 respiration in food industries (bread, tapai, and
                                                 yogurt).

6   Photosynthesis                  16

    6.1   Autotroph                  3       Candidates should be able to:
                                             (a) classify autotroph into photoautotroph and
                                                 chemoautotroph;
                                             (b) describe photosynthetic pigments;
                                             (c) explain the absorption spectrum and action
                                                 spectrum of photosynthetic pigments.

    6.2   Light-dependent            3       Candidates should be able to:
          reactions
                                             (a) explain photo-activation of chlorophyll a
                                                 resulting in photolysis of water;
                                             (b) explain the cyclic and non-cyclic
                                                 photophosphorylation including electron
                                                 transport system resulting in the production of
                                                 ATP and NADPH.




                                             7
Teaching
         Topic                                       Learning Outcome
                           Period

6.3   Light-independent      8       Candidates should be able to:
      reactions
                                     (a) describe Calvin cycle;
                                     (b) explain photorespiration;
                                     (c) describe the anatomical structure of C4 leaf
                                         (Krantz anatomy) in comparison to C3 leaf;
                                     (d) explain carbon dioxide fixation in C4 plants
                                         and Crassulacean Acid Metabolism (CAM)
                                         plants;
                                     (e) differentiate the metabolism of C3, C4 and
                                         CAM plants.

6.4   Limiting factors       2       Candidates should be able to:

                                     (a) explain limiting factors of photosynthesis
                                         (light intensity, carbon dioxide concentration
                                         and temperature);
                                     (b) relate the roles of C3, C4 and CAM plants on
                                         the increasing carbon dioxide emission and
                                         global warming.




                                     8
SECOND TERM: PHYSIOLOGY

                                Teaching
             Topic                                          Learning Outcome
                                 Period

7   Gas Exchange                  12

    7.1   Gaseous exchange in      7       Candidates should be able to:
          humans
                                           (a) outline the structure of human respiratory
                                               system, including the microscopic structure of
                                               the wall of an alveolus;
                                           (b) describe the structure of haemoglobin;
                                           (c) explain the transport of oxygen and carbon
                                               dioxide in blood;
                                           (d) explain the oxygen dissociation curves of
                                               haemoglobin, myoglobin and foetal
                                               haemoglobin;
                                           (e) explain the Bohr effect and relate it to the
                                               oxygen dissociation curve.

    7.2   Breathing cycle          3       Candidates should be able to:

                                           (a) explain the control of breathing mechanism,
                                               including the role of chemoreceptor;
                                           (b) define tidal volume, vital capacity, total lung
                                               capacity, inspiratory reserve volume,
                                               expiratory reserve volume and residual
                                               volume.

    7.3   Gaseous exchange in      2       Candidates should be able to:
          plants
                                           (a) describe the structure and functions of stomata;
                                           (b) describe the mechanism of opening and
                                               closing of stomata based on potassium ion
                                               accumulation hypothesis.

8   Transport in Animals and      16
    Plants

    8.1   Transport system in      8       Candidates should be able to:
          mammals
                                           (a) describe the structure of a mammalian heart;
                                           (b) define systole and diastole, and explain the
                                               sequence of events in a cardiac cycle including
                                               changes in pressure and volume in aorta, left
                                               atrium and left ventricle;
                                           (c) describe the initiation and regulation of heart
                                               beat;




                                           9
Teaching
             Topic                                           Learning Outcome
                                 Period

                                           (d) explain hypertension, atherosclerosis,
                                               arteriosclerosis and myocardial infarction, and
                                               state their causes and preventions;
                                           (e) describe the lymphatic system in relation to the
                                               blood circulatory system;
                                           (f)   determine the direction of fluid movement at
                                                 the arterial and venous ends of the capillaries
                                                 by calculating the differences between
                                                 osmotic pressure/solute potential and
                                                 hydrostatic pressure.

    8.2   Transport system in      8       Candidates should be able to:
          vascular plants
                                           (a) explain the uptake of water and mineral ions
                                               from the soil by the root hairs involving water
                                               potential;
                                           (b) describe the apoplast, symplast and vacuolar
                                               pathway of water movement through the root
                                               tissues;
                                           (c) describe the root pressure, cohesion-tension
                                               theory and transpiration pull in relation to
                                               water movement from the roots to leaves;
                                           (d) explain translocation using the mass flow,
                                               electro-osmosis, cytoplasmic streaming and
                                               peristaltic waves hypotheses;
                                           (e) explain the concept of source and sink, and
                                               phloem loading and unloading in translocation
                                               according to pressure flow hypothesis.

9   Control and Regulation        22

    9.1   Nervous system          16       Candidates should be able to:

                                           (a) describe the organisation of the nervous
                                               system in humans;
                                           (b) explain the formation of resting and action
                                               potentials;
                                           (c) describe the characteristics of nerve impulse;
                                           (d) describe the structure of synapse, and explain
                                               the role of neurotransmitters (acetylcholine
                                               and norepinephrine);
                                           (e) explain and compare the mechanisms of
                                               impulse transmission along the axon and
                                               across the synapse;




                                           10
Teaching
            Topic                                            Learning Outcome
                                  Period

                                            (f)   describe the structure of neuromuscular
                                                  junction and sarcomere;
                                            (g) explain the role of sarcoplasmic reticulum,
                                                calcium ions, myofibril and T tubules in
                                                muscle contraction;
                                            (h) explain the mechanism of muscle contraction
                                                according to the sliding filament hypothesis;
                                            (i)   compare the sympathetic and parasympathetic
                                                  nervous systems;
                                            (j)   explain the mechanisms of drug action on
                                                  nervous system and neuromuscular junction
                                                  (cocaine and curare).

   9.2   Hormones                   6       Candidates should be able to:

                                            (a) explain the mechanisms of action of steroid
                                                hormone and non-steroid hormones;
                                            (b) explain the roles of plant hormones in growth
                                                and development;
                                            (c) explain the mechanism of phytochrome action
                                                and their roles in photoperiodism and
                                                flowering;
                                            (d) outline the application of plant growth
                                                regulators (synthetic auxin, synthetic
                                                gibberellins, and synthetic ethylene) in
                                                agriculture.

10 Reproduction,                   13
   Development and Growth

   10.1 Sexual reproduction in      6       Candidates should be able to:
        humans
                                            (a) outline spermatogenesis and oogenesis;
                                            (b) describe the passage and development of
                                                sperms from the testis to the oviduct for
                                                fertilisation;
                                            (c) describe the process of fertilisation and
                                                implantation;
                                            (d) describe the roles of hormones in menstrual
                                                cycle and pregnancy;
                                            (e) describe briefly the stages in embryonic
                                                development;




                                            11
Teaching
            Topic                                             Learning Outcome
                                  Period

                                            (f)   explain the roles of placenta, chorion, amniotic
                                                  fluid and allantois in foetal development;
                                            (g) explain the process of parturition.

   10.2 Sexual reproduction in      2       Candidates should be able to:
        flowering plants
                                            (a) outline double fertilisation;
                                            (b) describe the embryonic development in seed
                                                and formation of fruit.

   10.3 Seed germination            1       Candidates should be able to:

                                            (a) explain the mobilisation of nutrients after
                                                imbibition in seed germination;
                                            (b) state the external factors affecting germination.

   10.4 Growth curves and           4       Candidates should be able to:
        patterns of growth
                                            (a) explain the types of growth curves (absolute
                                                growth curve, absolute growth rate curve and
                                                relative growth rate curve);
                                            (b) explain with examples the patterns of growth
                                                (limited growth in humans, unlimited growth
                                                in perennial plant, allometric growth in
                                                humans, isometric growth in fish and
                                                intermittent growth in insect);
                                            (c) explain the processes of ecdysis and
                                                metamorphosis in insects, and relate the role of
                                                hormones (neurosecretory hormone, juvenile
                                                hormone and ecdysone) in these processes.

11 Homeostasis                     10

   11.1 Importance of               2       Candidates should be able to:
        homeostasis
                                            (a) explain the importance of homeostasis;
                                            (b) describe the homeostatic control system in
                                                mammals;
                                            (c) explain the physiological and behavioural
                                                control in thermoregulation of endotherms.




                                            12
Teaching
           Topic                                        Learning Outcome
                             Period

   11.2 Liver                  4       Candidates should be able to:

                                       (a) describe the structure of liver, and explain the
                                           roles of its components;
                                       (b) describe carbohydrate metabolism in the liver
                                           (glycogenesis, glycogenolysis,
                                           gluconeogenesis);
                                       (c) describe protein metabolism (transamination,
                                           deamination and urea formation) in the liver.

   11.3 Osmoregulation in      3       Candidates should be able to:
        mammals
                                       (a) explain the process of ultrafiltration,
                                           reabsorption and secretion in the formation of
                                           urine;
                                       (b) explain the role of ADH and aldosterone, and
                                           the related hormones in regulating water,
                                           sodium and potassium ions of urine;
                                       (c) explain the regulation of pH of tissue fluid.

   11.4 Osmoregulation in      1       Candidates should be able to:
        plants
                                       (a) describe the role of stomata in regulation of
                                           water loss, and explain the importance of
                                           transpiration;
                                       (b) describe the various types of plant adaptations
                                           to prevent water loss (halophytes and
                                           xerophytes).

12 Immunity                   10

   12.1 Immune system          3       Candidates should be able to:
                                       (a) describe human lymphatic system, and explain
                                           its function in relation to immunity;
                                       (b) describe antibody (structure and function),
                                           antigen, epitope, and the development of B
                                           and T cells;
                                       (c) describe the roles of macrophages, B cells and
                                           T cells.




                                       13
Teaching
            Topic                                         Learning Outcome
                               Period

   12.2 Development of           3       Candidates should be able to:
        immunity
                                         (a) explain cell-mediated and humoral immune
                                             responses;
                                         (b) outline the antigen-antibody reactions
                                             (precipitation, agglutination, neutralisation,
                                             complement fixation).

   12.3 Concept of self and      3       Candidates should be able to:
        non-self
                                         (a) explain the concept of self and non-self and
                                             relate this to tissue rejection in organ
                                             transplant;
                                         (b) explain the mechanism of immune suppression
                                             (HIV infection).

   12.4 Immune disorder          1       Candidates should be able to:

                                         (a) describe autoimmune disorder (Systemic Lupus
                                             Erythematosus (SLE)).

13 Infectious Diseases          13

   13.1 Infectious disease       1       Candidates should be able to:
                                         (a) explain what is meant by an infection and an
                                             infectious disease;
                                         (b) outline the types of infectious agents.

   13.2 Dengue                   3       Candidates should be able to:

                                         (a) describe the causes and symptoms of dengue;
                                         (b) explain the transmission of dengue;
                                         (c) discuss the roles of social, economical and
                                             biological factors in the prevention of dengue.

   13.3 Cholera                  3       Candidates should be able to:

                                         (a) describe the causes and symptoms of cholera;
                                         (b) explain the transmission of cholera;
                                         (c) discuss the roles of social, economical and
                                             biological factors in the prevention of cholera.




                                         14
Teaching
        Topic                                       Learning Outcome
                          Period

13.4 Tuberculosis (TB)      3       Candidates should be able to:

                                    (a) describe the causes and symptoms of
                                        tuberculosis (TB);
                                    (b) explain the transmission of TB;
                                    (c) discuss the roles of social, economical and
                                        biological factors in the prevention of TB.

13.5 Malaria                3       Candidates should be able to:

                                    (a) describe the causes and symptoms of malaria;
                                    (b) explain the transmission of malaria;
                                    (c) discuss the roles of social, economical and
                                        biological factors in the prevention of malaria.




                                    15
THIRD TERM: ECOLOGY AND GENETICS

                                  Teaching
            Topic                                             Learning Outcome
                                   Period

14 Taxonomy and                     14
   Biodiversity

   14.1 Taxonomy                     2       Candidates should be able to:
                                             (a) explain the importance of taxonomy in
                                                 biological sciences;
                                             (b) explain the concept of species, and relate how
                                                 a species is classified into higher categories in
                                                 a taxonomic hierarchy.

   14.2 Diversity of organisms       6       Candidates should be able to:

                                             (a) describe the morphological characteristics of
                                                 the following phyla in the respective
                                                 kingdoms: Protoctista (Chlorophyta and
                                                 Zoomastigina), Fungi (Zygomycota), Plantae
                                                 (Bryophyta, Filicinophyta, Coniferophyta and
                                                 Angiospermophyta) and Animalia (Porifera,
                                                 Cnidaria, Platyhelminthes, Mollusca,
                                                 Arthropoda and Chordata).

   14.3 Biodiversity in              2       Candidates should be able to:
        Malaysia
                                             (a) describe the different levels and examples of
                                                 biodiversity in Malaysia, namely ecosystem or
                                                 community diversity, species or taxonomic
                                                 diversity and genetic diversity;
                                             (b) explain the importance of biodiversity in
                                                 Malaysia.

   14.4 Threats to biodiversity      2       Candidates should be able to:

                                             (a) explain the natural and man-made factors that
                                                 threaten biodiversity in Malaysia;
                                             (b) explain the steps and efforts taken by various
                                                 agencies and organisations to address the
                                                 threats.

   14.5 Conservation of              2       Candidates should be able to:
        biodiversity
                                             (a) describe the various measures taken to
                                                 conserve the different levels of biodiversity
                                                 including in situ and ex situ conservation in
                                                 Malaysia.




                                             16
Teaching
             Topic                                          Learning Outcome
                                 Period

15 Ecology                        22

   15.1 Levels of ecological       3       Candidates should be able to:
        organisation
                                           (a) explain the concept of hierarchy in an
                                               ecosystem and the interaction between the
                                               biotic and abiotic components.

   15.2 Biogeochemical cycles      3       Candidates should be able to:

                                           (a) describe the biogeochemical cycles (carbon,
                                               phosphorus and sulphur), and explain their
                                               importance.

   15.3 Energy flow                3       Candidates should be able to:

                                           (a) describe the energy flow and the efficiency of
                                               energy transfer in terrestrial ecosystem
                                               (tropical rain forest) and aquatic ecosystem
                                               (lake).

   15.4 Population ecology         6       Candidates should be able to:

                                           (a) explain population growth (S and J growth
                                               curves), biotic potential, natality, mortality,
                                               migration and survivorship;
                                           (b) explain the characteristics of populations that
                                               show Type I, Type II and Type III
                                               survivorship curves, and K-strategies and
                                               r-strategies.

   15.5 Carrying capacity          3       Candidates should be able to:

                                           (a) explain what is meant by carrying capacity and
                                               sustainable development;
                                           (b) explain the factors limiting the population size
                                               and distribution.

   15.6 Quantitative ecology       4       Candidates should be able to:

                                           (a) describe the use of quadrat and line transect
                                               sampling methods and explain the advantages
                                               and disadvantages of using these methods;
                                           (b) calculate the various sampling parameters
                                               (frequency, density, cover and their absolute
                                               and relative estimations) and estimate the
                                               population size of organisms;
                                           (c) explain the pattern of distribution of organisms
                                               in an ecosystem.



                                           17
Teaching
            Topic                                            Learning Outcome
                                  Period

16 Selection and Speciation        10

   16.1 Natural and artificial      6       Candidates should be able to:
        selection
                                            (a) describe continuous and discontinuous
                                                variations in relation to selection and
                                                speciation;
                                            (b) explain the modes of natural selection
                                                (stabilising, directional and disruptive) and
                                                their consequences;
                                            (c) describe with examples, sexual selection and
                                                polymorphism;
                                            (d) explain the importance of artificial selection
                                                (gene bank, germplasm bank and sperm bank).

   16.2 Speciation                  4       Candidates should be able to:

                                            (a) explain the processes of isolation, genetic drift,
                                                hybridisation and adaptive radiation;
                                            (b) explain the importance of speciation in relation
                                                to evolution.

17 Inheritance and Genetic         34
   Control

   17.1 Types of genetic            5       Candidates should be able to:
        crosses and breeding
                                            (a) explain the Mendelian inheritance pertaining
        system
                                                to the phenotypic and genotypic ratios;
                                            (b) describe the types of crosses (test cross,
                                                backcross, reciprocal cross and selfing) and
                                                explain their importance;
                                            (c) describe pure breeding, outbreeding,
                                                inbreeding, selective breeding, and explain
                                                their importance.

   17.2 Non-Mendelian               7       Candidates should be able to:
        inheritance
                                            (a) explain
                                                  (i) incomplete dominance (flower colour in
                                                      snapdragon),
                                                  (ii) codominance (MN blood group in
                                                       humans),
                                                 (iii) multiple alleles (ABO blood group in
                                                       humans), and
                                                 calculate the genotypic and phenotypic ratios;




                                            18
Teaching
         Topic                                        Learning Outcome
                            Period

                                      (b) explain lethal genes (sickle-cell in human/coat
                                          colur in mice/chlorophyll production in
                                          maize), polygenes (height in humans), linked
                                          and sex-linked genes (Drosophila eye colour
                                          and haemophilia in humans), and epistasis
                                          (coat colour in dog and capsule shape in
                                          shepherd’s purse plant);
                                      (c) explain the pedigree analysis.

17.3 Genetic mapping          2       Candidates should be able to:

                                      (a) explain crossing over and distinguish between
                                          parental and recombinant genotypes and
                                          phenotypes;
                                      (b) calculate the distance between two loci, and
                                          determine the relative position of a gene on a
                                          chromosome based on percentage of crossing-
                                          over in Drosophila.

17.4 Population genetics      5       Candidates should be able to:

                                      (a) describe the concept of gene pool, gene/allele
                                          frequency and genotype frequency;
                                      (b) explain Hardy-Weinberg equilibrium
                                          (p2 + 2pq +q2 = 1 and p + q = 1), and calculate
                                          the gene/allele and genotype frequencies;
                                      (c) explain the conditions for Hardy-Weinberg
                                          equilibrium to be valid;
                                      (d) describe changes in genotype frequencies in
                                          relation to evolution.

17.5 DNA replication          4       Candidates should be able to:

                                      (a) explain the experiments to prove DNA is the
                                          genetic material (Avery, MacLeod and
                                          McCarty experiment and Hershey and Chase
                                          experiment);
                                      (b) explain the three models of DNA replication,
                                          and interpret the experiment of Meselson and
                                          Stahl to prove the semi-conservative model of
                                          DNA replication;
                                      (c) explain the mechanism of DNA replication,
                                          and the role of the enzymes involved.




                                      19
Teaching
        Topic                                         Learning Outcome
                           Period

17.6 Gene expression         4       Candidates should be able to:

                                     (a) explain the experiment of Beadle and Tatum
                                         which leads to the establishment of one-gene-
                                         one-polypeptide hypothesis;
                                     (b) interpret the genetic code table, and identify
                                         the appropriate anti-codon;
                                     (c) explain the characteristics of genetic code;
                                     (d) describe transcription and translation.

17.7 Regulation of gene      2       Candidates should be able to:
     expression
                                     (a) define repressor, inducer, negative control in
                                         lac operon and constitutive enzyme;
                                     (b) describe the components of lac operon, and
                                         explain its mechanism.

17.8 Mutation                5       Candidates should be able to:

                                     (a) describe the different types of gene mutation
                                         with examples of its consequences
                                         (substitution – sickle-cell anaemia,
                                         insertion/addition – frameshift mutation,
                                         deletion – frameshift mutation and
                                         thalassaemia major and inversion);
                                     (b) differentiate missense, nonsense and silent/
                                         neutral mutations;
                                     (c) describe the four structural changes in
                                         chromosomes (duplication, deletion, inversion
                                         and translocation);
                                     (d) describe the changes in chromosome number,
                                         including the definition of non-disjunction;
                                     (e) describe the consequences of non-disjunction
                                         in relation to meiosis;
                                     (f)   explain and give examples of different types of
                                           aneuploidy (monosomy and trisomy);
                                     (g) explain and give examples of different types of
                                         euploidy: diploid and polyploid, including
                                         autopolyploidy and allopolyploidy.




                                     20
Teaching
            Topic                                     Learning Outcome
                           Period

18 Gene Technology           8

   18.1 Recombinant DNA      8       Candidates should be able to:
        technology
                                     (a) explain recombinant DNA technology/genetic
                                         engineering;
                                     (b) differentiate between genomic and cDNA
                                         cloning and genomic and cDNA libraries;
                                     (c) describe the vectors used in cloning and their
                                         properties;
                                     (d) describe the restriction enzyme (EcoR1 and
                                         SmaI), including its nomenclature, recognition
                                         site (palindrome), importance and the types of
                                         ends generated;
                                     (e) explain reverse transcription, insertion,
                                         ligation, transformation/transduction,
                                         amplification and screening;
                                     (f)   describe the steps involved in genomic and
                                           cDNA cloning, including the enzymes
                                           involved, and explain human insulin
                                           production in E. coli as an example.

19 Biotechnology             8

   19.1 Roles of             1       Candidates should be able to:
        biotechnology
                                     (a) define biotechnology;
                                     (b) outline the roles of biotechnology in our life.

   19.2 Applications of      7       Candidates should be able to:
        biotechnology
                                     (a) describe the application of biotechnology in
                                         food and beverages production (fermentation
                                         and vitamin-enriched eggs);
                                     (b) describe the application of biotechnology in
                                         agriculture (hybrid rice, herbicide resistant
                                         plants and transgenic fish);
                                     (c) describe the application of biotechnology in
                                         medicine (human growth hormone, human
                                         insulin and gene therapy) and forensic (DNA
                                         finger printing);
                                     (d) describe the application of biotechnology in
                                         public health (genetic screening, diagnostic
                                         kits and oil-decomposing bacteria).




                                     21
The Practical Syllabus
School-based Assessment of Practical (Paper 4)

School-based assessment of practical work will be carried out throughout the form six school terms
for candidates from government schools and private schools which are approved by the MEC to carry
out the school-based assessment of practical work.

    MEC will determine 15 compulsory experiments to be carried out by the candidates and to be
assessed by the subject teachers in schools in the respective terms. Details of the title, objective,
learning outcome, introduction, material, apparatus and procedure of each of the experiments will be
specified in the Teacher’s and Student’s Manual for Practical Biology which can be downloaded from
MEC Portal (http://www.mpm.edu.my) during the first term of form six by the subject teacher.

    Candidates should be supplied with a work scheme before the day of the compulsory experiment
so as to enable them to plan their practical work. Each experiment is expected to last one school
double period. Assessment of the practical work is done by the subject teachers during the practical
sessions and also based on the practical reports. The assessment should comply with the assessment
guidelines prepared by MEC.

    A repeating candidate may use the total mark obtained in the coursework for two subsequent
examinations. Requests to carry forward the moderated coursework mark should be made during the
registration of the examination.

    During the practical session, candidates should be able to:
    (a)   make observations on living, preserved specimens and models, and record them accurately
          in writing or by using clear drawings or diagrams,
    (b)   identify morphological features of specimens as a basis for their classification,
    (c)   carry out dissection, display and illustrate the result,
    (d)   stain and mount specimens for microscopic examination,
    (e)   identify and illustrate the specimens observed under light microscope,
    (f)   carry out biochemical, physiological and genetic experiments,
    (g)   carry out sampling for ecological studies,
    (h)   record the results systematically, interpret the data scientifically and make conclusion.




                                                    22
Written Practical Test (Paper 5)

The main objective of written practical test is to assess the candidates’ understanding of practical
procedures in the laboratory.

    The following candidates are required to register for this paper:
    (a)   individual private candidates,
    (b)   candidates from private schools which have no permission to carry out the school-based
          assessment of practical work,
    (c)   candidates who repeat upper six (in government or private schools),
    (d)   candidates who do not attend classes of lower six and upper six in two consecutive years
          (in government or private schools),
    (e)   candidates who take Biology other than the package offered by schools.

    Three structured questions related to practical work will be set. MEC will not be strictly bound by
the syllabus in setting questions. Where appropriate, candidates will be given sufficient information to
enable them to answer the questions. Only knowledge of theory within the syllabus and knowledge of
usual laboratory practical procedures will be expected.

    Candidates should have sufficient knowledge on:
    (a)   structure of specimens and record them accurately in writing or illustration,
    (b)   morphological and anatomical features and relate to functions of specimens as a basis for
          their classification,
    (c)   dissection of specimens and illustration of the result (rat),
    (d)   staining and mounting specimens for microscopic examination,
    (e)   identification and illustration of the specimens as observed under microscope,
    (f)   biochemical, physiological and genetic experiments,
    (g)   sampling for ecological studies,
    (h)   interpreting, evaluating, drawing conclusion from experimental data,
    (i)   using theories to explain the results of experiments and performing simple calculations
          based on experiments.




                                                    23
Scheme of Assessment

 Term of    Paper Code                                             Mark
                         Theme/Title        Type of Test                       Duration   Administration
  Study      and Name                                            (Weighting)
  First       964/1      Biological    Written test                  60
  Term       Biology     Molecules                                (26.67%)
             Paper 1     and
                                       Section A                     15
                         Metabolism
                                       15 compulsory
                                       multiple-choice
                                       questions to be
                                       answered.
                                       Section B                     15
                                                                                             Central
                                       2 compulsory short                      1½ hours
                                                                                           assessment
                                       structured questions to
                                       be answered.
                                       Section C                     30
                                       2 out of 3 essay
                                       questions to be
                                       answered.
                                       All questions are
                                       based on topics 1 to 6.

 Second       964/2      Physiology    Written test                  60
  Term       Biology                                              (26.67%)
             Paper 2
                                       Section A                     15
                                       15 compulsory
                                       multiple-choice
                                       questions to be
                                       answered.
                                       Section B                     15
                                       2 compulsory short                                    Central
                                                                               1½ hours
                                       structured questions to                             assessment
                                       be answered.
                                       Section C                     30
                                       2 out of 3 essay
                                       questions to be
                                       answered.
                                       All questions are
                                       based on topics 7 to
                                       13.




                                                 24
Term of   Paper Code                                             Mark
                       Theme/Title        Type of Test                       Duration   Administration
 Study     and Name                                            (Weighting)
Third       964/3      Ecology and   Written test                  60
Term       Biology     Genetics                                 (26.67%)
           Paper 3
                                     Section A                     15
                                     15 compulsory
                                     multiple-choice
                                     questions to be
                                     answered.
                                     Section B                     15
                                     2 compulsory short                                    Central
                                                                             1½ hours
                                     structured questions to                             assessment
                                     be answered.
                                     Section C                     30
                                     2 out of 3 essay
                                     questions to be
                                     answered.
                                     All questions are
                                     based on topics 14 to
                                     19.

            964/5                    Written Practical             45
           Biology                   Test                        (20%)
           Paper 5                                                                         Central
                                     3 structured questions                  1½ hours
                                                                                         assessment
                                     with diagram/graph/
                                     table to be answered.

 First,     964/4      Biology       School-based                 225
Second     Biology     Practical     Assessment of               (20%)
  and                                                                        Through-
           Paper 4                   Practical
Third                                                                         out the   School-based
Terms                                15 compulsory                             three     assessment
                                     experiments to be                         terms
                                     carried out.




                                                 25
Performance Descriptions

A Grade A candidate is likely able to:
    (a)   recall almost all the content of required areas of biological syllabus, and relate the content
          with the question;
    (b)   decide on, organise and present information to explain the concept, theories and depth of
          biological knowledge in an ordered and logical manner;
    (c)   plan and carry out investigations, solve problems based on a sound biological knowledge;
    (d)   interpret and analyse complex data presented, and relate the biological knowledge and
          understanding of content by using correct formulae, terminologies, scientific units and
          approach;
    (e)   integrate various concepts and findings accurately, and formulate logical conclusion;
    (f)   apply and justify knowledge and understanding successfully to unfamiliar contexts and
          data.


A Grade C candidate is likely able to:
    (a)   recall some of the content of the required areas of biological syllabus, and relate the content
          with the question moderately;
    (b)   decide on, organise and present information to describe the concept, theories and depth of
          biological knowledge;
    (c)   plan and carry out investigations, solve problems based on a mediocre biological
          knowledge;
    (d)   interpret and analyse simple and straight forward data presented, and relate the biological
          knowledge and understanding of content by using some formulae, terminologies and
          scientific units;
    (e)   integrate some concepts and findings, and formulate simple conclusion;
    (f)   apply knowledge with moderate understanding to unfamiliar contexts and data.




                                                  26
Reference Books

Teachers and candidates may use books specially written for the STPM examination and other
reference books such as those listed below.

1.    Alters, S. and Alters, B., 2006. Biology: Understanding Life. New Jersey: John Wiley & Sons,
         Inc.

2.    Audesirk, T., Audesirk, G. and Byers, B. E., 2001. Biology: Life on Earth. 6th edition. New
        Jersey: Prentice Hall.

3.    Campbell, M. K., Farrell, S. O., 2006. Biochemistry. Belmont: Thomson Brooks/Cole.

4.    Campbell, N. A. and Reece, N. A., 2005. Biology. 7th edition. San Francisco: Benjamin/
        Cummings.

5.    Clegg, C. J. and Mackean, D. G., 2000. Advanced Biology: Principles and Applications.
         2nd edition. London: John Murray.

6.    Kent, M., 2000. Advanced Biology. Oxford: Oxford University Press.

7.    Klug, W. S., Cummings, M.R. and Spencer, C. A., 2005. Concepts of Genetics. 8th edition.
         New Jersey: Pearson Prentice Hall.

8.    Klug, W. S. and Cummings, M. R., 2005. Essentials of Genetics. 5th edition. New Jersey:
         Pearson Prentice Hall.

9.    Mader, S., 2004. Biology. 8th edition. New York: McGraw-Hill.

10.   Raven, P. H., et. al. 2005. Biology. 7th edition. New York: McGraw-Hill.

11.   Russel, P. J., et. al. 2008. Biology: The Dynamics Science. 1st edition. Belmont: Thompson
        Brooks/Cole.

12.   Smith, R. L. and Smith, T. M., 2003. Elements of Ecology. 5th edition. San Francisco: Benjamin
        Cummings.

13.   Solomon, E. P., Berg, L. R. and Martin, D. W., 2005. Biology. 7th edition. Belmont: Thompson
         Brooks/Cole.

14.   Taylor, D. J., Green, N. P. O. and Stout, G. W., 2003. Biological Science 1: Organisms,
        Energy and Environment. 3rd edition. Cambridge: Cambridge University Press.

15.   Taylor, D. J., Green, N. P. O. and Stout, G. W., 2003. Biological Science 2: Systems,
        Maintenance and Change. 3rd editon. Cambridge: Cambridge University Press.

16.   Toole, G. and Toole, S., 1999. Understanding Biology for Advanced Level. 4th edition. United
        Kingdom: Nelson Thornes.




                                                27
28
Identity card number:………………………….. Centre number/index number:……………………….
(Nombor kad pengenalan)           (Nombor pusat/angka giliran)



                              SPECIMEN PAPER

       964/1                                                                  STPM

                                   BIOLOGY (BIOLOGI)

                                   PAPER 1 (KERTAS 1)
                              One and a half hours (Satu jam setengah)


                       MAJLIS PEPERIKSAAN MALAYSIA
                              (MALAYSIAN EXAMINATIONS COUNCIL)



                 SIJIL TINGGI PERSEKOLAHAN MALAYSIA
                          (MALAYSIA HIGHER SCHOOL CERTIFICATE)


Instructions to candidates:
    DO NOT OPEN THIS QUESTION PAPER UNTIL YOU ARE TOLD TO DO SO.
    Answer all questions in Section A. Marks will not be deducted for wrong answers. For each
question, four suggested answers are given. Choose the correct answer and circle the answer.
    Answer all questions in Section B. Write your answers in the spaces provided.
    Answer any two questions in Section C. All essential working should be shown. For numerical
answers, unit should be quoted wherever appropriate. Begin each answer on a fresh sheet of paper
and arrange your answers in numerical order.

Arahan kepada calon:
    JANGAN BUKA KERTAS SOALAN INI SEHINGGA ANDA DIBENARKAN BERBUAT
DEMIKIAN.
    Jawab semua soalan dalam Bahagian A. Markah tidak akan ditolak bagi jawapan yang salah.
Bagi setiap soalan, empat cadangan jawapan diberikan. Pilih jawapan yang betul dan buat bulatan
pada jawapan tersebut.
    Jawab semua soalan dalam Bahagian B. Tulis jawapan anda di ruang yang diberikan.
    Jawab mana-mana dua soalan dalam Bahagian C. Semua jalan kerja yang sesuai hendaklah
ditunjukkan. Bagi jawapan berangka, unit hendaklah dinyatakan di mana-mana yang sesuai.
Mulakan setiap jawapan pada helaian kertas jawapan yang baharu dan susun jawapan anda
mengikut tertib berangka.

              This question paper consists of printed pages and          blank page.
       (Kertas soalan ini terdiri daripada halaman bercetak dan           halaman kosong.)
                                   © Majlis Peperiksaan Malaysia
STPM 964/1


                                                29
Section A [15 marks]

                                  Answer all questions in this section.

1   Which property of water is important to living organisms?
    A Polar molecule
    B Non polar molecule
    C Low specific heat capacity
    D Low latent heat of vaporisation

2   Which monosaccharide, example and its description correspond?
        Monosaccharide            Example                                 Description
    A       Pentose               Ribose                     A coenzyme involved in the transfer of
                                                             hydrogen during glycolysis
    B       Pentose               Ribulose                   A source of NADPH production
    C       Triose                Glyceraldehyde             An important intermediate substance in
                                                             glycolysis
    D       Triose                Dihydroxyacetone           A monomer of cellulose

3   Which is not true of lipid?
    A It is soluble in water.
    B It is a solvent for cholesterol.
    C It is soluble in organic solvents.
    D It is an ester formed from the reaction of fat and alcohol.

4   Which amino acid is the first to be carried by tRNA to ribosome during translation?
    A Leucine
    B Tyrosine
    C Tryptophan
    D Methionine

5   Which organelle gives rise to the membranes of a cell?
    A Vacuole
    B Nucleus
    C Lysosome
    D Smooth endoplasmic reticulum




964/1


                                                   30
Bahagian A [15 markah]

                             Jawab semua soalan dalam bahagian ini.

1   Sifat air yang manakah yang penting kepada organism hidup?
    A Molekul berkutub
    B Molekul tak berkutub
    C Muatan haba tentu yang rendah
    D Haba pendam pengewapan yang rendah

2   Monosakarida, contohnya, dan penerangan yang manakah yang berpadanan?
        Monosakarida             Contoh                               Penerangan
    A      Pentosa               Ribosa                  Satu koenzim yang terlibat dalam
                                                         pemindahan hydrogen semasa glikolisis
    B      Pentosa               Ribulosa                Sumber penghasilan NADPH
    C      Triosa                Gliseraldehid           Bahan perantara penting dalam glikolisis
    D      Triosa                Dihidroksiaseton        Monomer selulosa

3   Yang manakah yang tidak benar tentang lipid?
    A Terlarut dalam air.
    B Pelarut bagi kolesterol.
    C Terlarut dalam pelarut organik.
    D Satu ester yang terbentuk daripada tindak balas lemak dan alkohol.

4   Asid amino yang manakah yang pertama dibawa oleh tRNA ke ribosom semasa translasi?
    A Leusina
    B Tirosina
    C Triptofan
    D Metionina

5   Organel yang manakah yang membentuk membran sel?
    A Vakuol
    B Nukleus
    C Lisosom
    D Jalinan endoplasma licin




964/1


                                                    31
6   Which statement is not true of xylem?
    A Xylem cells contain pits.
    B Matured xylem cells are dead cells.
    C Companion cells provide nutrients to the xylem.
    D Xylem consists of vessel elements and tracheids.

7   The table below shows the components of a cell membrane and their functions.

        Component of a cell
                                                           Functions
           membrane

          I Oligosaccharide        p Acts as a receptor to a certain molecule such as hormone
         II Lecithin               q As an indicator so that the cells can recognise each other
        III Protein                r Gives the semi-permeable and selective characteristics to
                                     the membrane
        IV Cholesterol             s Maintains the fluid characteristics of the membrane

    Which components of cell membrane and functions correspond?
          I     II     III    IV
    A    p      q      r      s
    B     q     r      p      s
    C     r     p      s      q
    D     s     r      q      p

8   Which factor reduces enzyme activity?
    A Extreme pH
    B Heavy metal
    C Competitive inhibitor
    D Very high temperature




964/1


                                                  32
6   Penyataan yang manakah yang tidak benar tentang xilem?
    A Sel xilem mengandungi pit.
    B Sel xylem yang matang ialah sel mati.
    C Sel rakan membekalkan nutrien kepada xilem.
    D Xilem terdiri daripada unsur salur dan trakeid.

7   Jadual di bawah menunjukkan komponen-komponen satu membran sel dan fungsinya.

            Komponen
                                                            Fungsi
           membran sel

         I Oligosakarida          p Bertindak sebagai reseptor kepada molekul tertentu seperti
                                    hormon
        II Lesitin                q Sebagai penanda supaya sel boleh mengecam antara satu
                                    sama lain
        III Protein               r Memberi sifat separa telap dan sifat memilih pada membran
        IV Kolesterol             s   Mengekalkan ciri bendalir bagi membran

    Yang manakah yang betul bagi komponen membran sel di atas dan fungsi masing-masing?
          I     II      III   IV
    A     p      q       r    s
    B     q      r       p    s
    C     r      p       s    q
    D     s      r       q    p

8   Faktor yang manakah yang mengurangkan aktiviti enzim?
    A pH yang melampau
    B Logam berat
    C Perencat persaingan
    D Suhu yang sangat tinggi




964/1


                                                  33
9 Which graph shows the possible changes in the rate of a reaction catalysed by an enzyme in the
presence of a competitive inhibitor?


           1/V      Without                                 1/V       With
                    inhibitor                                         inhibitor

                                With                                        Without
                                inhibitor                                   inhibitor



                                       1/[S]                                            1/[S]
                         A                                                 B


          1/V Without                                       1/V
                 inhibitor                                             With
                                  With
                                                                       inhibitor
                                  inhibitor
                                                                                   Without
                                                                                   inhibitor



                                      1/[S]                                             1/[S]
                         C                                                 D

10 Which statement describes the advantage of immobilised enzyme?
    A The efficiency of enzyme increases.
    B The enzyme can be easily retrieved but cannot be reused.
    C The end product does not contain the enzyme and easily retrieved.
    D The characteristics of enzyme can be changed in order to increase its surface area.

11 What is the fate of all carbons from a glucose molecule after it is completely oxidised?
    A Pyruvate
    B Carbohydrate
    C Carbon dioxide
    D Acetyl coenzyme A

12 In a condition without oxygen, pyruvate in plants will
    A be converted into lactate
    B be converted into acetaldehyde
    C enter the Krebs cycle immediately
    D be converted back into phosphoglyceraldehyde




964/1


                                                  34
9 Graf yang manakah yang menunjukkan kemungkinan perubahan kadar tindak balas yang
dimangkinkan oleh enzim dalam kehadiran perencat persaingan?


        1/V    Tanpa                                 1/V          Dengan
               perencat                                           perencat
                            Dengan
                            perencat                                   Tanpa
                                                                       perencat



                                1/[S]                                             1/[S]
                       A                                               B

        1/V Tanpa                                    1/V
            perencat                                              Dengan
                            Dengan                                perencat
                            perencat                                         Tanpa
                                                                             perencat



                                1/[S]                                             1/[S]
                       C                                               D

10 Penyataan yang manakah yang memerihalkan kelebihan enzim pentakmobilan?
   A Keefisienan enzim bertambah.
   B Enzim boleh didapatkan dengan mudah tetapi tidak boleh digunakan semula.
   C Produk akhir tidak mengandungi enzim dan mudah didapatkan.
   D Ciri enzim boleh diubah bagi meningkatkan luas permukaan.

11 Apakah yang terjadi kepada semua karbon dari molekul glukosa selepas dioksidakan dengan
lengkap?
   A Piruvat
   B Karbohidrat
   C Karbon dioksida
   D Asetil koenzim A

12 Dalam keadaan ketiadaan oksigen, piruvat dalam tumbuhan akan
   A ditukarkan kepada laktat
   B ditukarkan kepada asetildehid
   C masuk ke kitar Krebs dengan segera
   D ditukar semula kepada fosfogliseraldehid




964/1


                                                35
13 In cyclic photophosphorylation, the excited electron which returns to its ground state will emit
    A light
    B microwave
    C gamma ray
    D ultraviolet radiation

14 Which statement describes the characteristic of bundle sheath cell of C4 plants?
    A It has small grana.
    B It contains a few starch granules.
    C The activity of photosystem I is high.
    D Carbon dioxide is assimilated by PEP carboxylase.

15 The diagram below shows non-cyclic photophosphorylation during the light reaction of
photosynthesis.




    Which are represented by X, Y and Z ?
            X                       Y                  Z
    A Ferredoxin              Plastoquinone      Plastocyanin
    B Ferredoxin              Plastocyanin       Plastoquinone
    C Plastocyanin            Plastoquinone      Ferredoxin
    D Plastoquinone           Plastocyanin       Ferredoxin




964/1


                                                  36
13 Dalam pemfotofostorilan berkitar, elektron teruja yang kembali ke keadaan asas akan
membebaskan
   A cahaya
   B gelombang mikro
   C sinar gamma
   D sinaran ultra lembayung

14 Penyataan yang manakah yang memerihalkan ciri sel berkas tumbuhan C4?
   A Mempunyai grana yang kecil.
   B Mengandungi beberapa granul kanji.
   C Aktiviti fotosistem I adalah tinggi.
   D Karbon dioksida diasimilasi oleh PEP karboksilase.

15 Gambar rajah di bawah menunjukkan pemfotofosforilan bukan kitar semasa tindak balas cahaya
fotosintesis.
                         Penerima                          Penerima
                         pertama                           pertama




                                            Kompleks
                                            sitokrom                  NADP+
                                                                      reduktase




          Cahaya                                                      Cahaya




   Yang manakah yang diwakili oleh X, Y, dan Z ?
           X                        Y                  Z
   A Ferredoksin           Plastokuinon         Plastosianin
   B Ferredoksin           Plastosianin         Plastokuinon
   C Plastosianin          Plastokuinon         Ferredoksin
   D Plastokuinon          Plastosianin         Ferredoksin




964/1


                                                 37
Section B [15 marks]

                                 Answer all questions in this section.

16 A quantity of cells taken from a type of tissue were homogenised and their organelles were
separated by ultracentrifugation. The diagrams below show three types of organelles obtained.




                                                                                 R
                                  P                          Q
                   Organelle X                 Organelle Y               Organelle Z

   (a) State where these cells may have been taken from.                                 [1 mark]

……………………………………………………………………………………………........................

   (b) Arrange the order of sedimentation of organelles X, Y and Z. Explain your answer. [2 marks]

……………………………………………………………………………………………........................

……………………………………………………………………………………………........................

   (c) From the above diagrams, name the structure labelled P, Q and R.                 [3 marks]

P: ………………………………………………………………………………………………………...

Q: ………………………………………………………………………………………………………...

R: ………………………………………………………………………………………………………...

   (d) Give one function of organelle Y.                                                 [1 mark]

……………………………………………………………………………………………........................

   (e) Give one common function of organelles X and Z.                                   [1 mark]

……………………………………………………………………………………………........................




964/1


                                                  38
Bahagian B [15 markah]

                            Jawab semua soalan dalam bahagian ini.

16 Sejumlah sel yang diambil daripada tisu telah dihomogenkan dan kandungan organelnya
diasingkan melalui ultrapengemparan. Gambar rajah di bawah menunjukkan tiga jenis organel yang
diperoleh.




                                                                             R
                                 P                          Q
                    Organel X                   Organel Y             Organel Z

   (a) Nyatakan dari manakah sel-sel ini mungkin telah diperoleh.                     [1 markah]

……………………………………………………………………………………………........................

   (b) Susunkan turutan pengenapan bagi organel X, Y, dan Z. Jelaskan jawapan anda.   [2 markah]

……………………………………………………………………………………………........................

……………………………………………………………………………………………........................

   (c) Daripada gambar rajah di atas, namakan struktur yang berlabel P, Q, dan R.     [3 markah]

P: ………………………………………………………………………………………………………...

Q: ………………………………………………………………………………………………………...

R: ………………………………………………………………………………………………………...

   (d) Berikan satu fungsi organel Y.                                                 [1 markah]

……………………………………………………………………………………………........................

   (e) Berikan satu fungsi sepunya bagi organel X dan Z.                              [1 markah]

……………………………………………………………………………………………........................




964/1


                                                 39
17 The diagram below shows photophosphorylation in light reaction for plants.

        Oxidation-
        reduction
        potential
        (Relative
        energy
        level)




    (a) Name the photosystems labeled Q and R, and give the wavelengths of their appropriate
reaction centres.                                                                 [4 marks]

        Name of photosystem                 Wavelength

        Q : ……………………                         ………………

        R: …………………….                        ………………

   (b) What are the forms of energy represented by S and T?                        [2 marks]

……………………………………………………………………………………………........................

……………………………………………………………………………………………........................

   (c) Name the process that occurs at Q.                                          [1 mark]

……………………………………………………………………………………………........................




964/1


                                                40
17 Gambar rajah di bawah menunjukkan pemfotofosforilan dalam tindak balas cahaya bagi
tumbuhan.

         Keupayaan
         pengoksidaan
         penurunan
         (Aras tenaga                                         Penerima elektron
         relatif)              Penerima elektron




    (a) Namakan fotosistem yang berlabel Q dan R, dan berikan panjang gelombang bagi pusat
tindak balas yang sewajarnya.                                                  [4 markah]

        Nama fotosistem              Panjang gelombang

        Q : ……………………                 ………………………..

        R: …………………….                 ………………………

   (b) Apakan bentuk tenaga yang diwakili oleh S dan T?                           [2 markah]

……………………………………………………………………………………………........................

   (c) Namakan proses yang berlaku di Q.                                          [1 markah]

……………………………………………………………………………………………........................




964/1


                                              41
Section C [30 marks]

                              Answer any two questions in this section.

18 (a) Describe the primary, secondary and tertiary structures of proteins. State the importance of
these structures which are related to the properties of proteins.                       [11 marks]
    (b) Based on their functions, proteins can be classified into several types. Give two types of these
proteins and their functions in living organisms.                                             [4 marks]

19 (a) Distinguish a bacteria chromosome from a eukaryotic chromosome.                         [8 marks]
    (b) Describe the distinctive anatomical features of vascular bundles in maize leaves.      [7 marks]

20 (a) Explain three different classes of enzymes based on the reactions that they catalyse.
                                                                                      [6 marks]
    (b) By giving two examples of enzymatic reactions, explain how the appropriate cofactor and
inhibitor affect their activities.                                                    [9 marks]




964/1


                                                  42
Bahagian C [30 markah]

                       Jawab mana-mana dua soalan daripada bahagian ini.

18 (a) Perihalkan struktur primer, sekunder, dan tertier bagi protein. Nyatakan kepentingan struktur
ini yang berkaitan dengan sifat protein.                                                [11 markah]
    (b) Berdasarkan fungsinya, protein boleh dikelaskan kepada beberapa jenis. Berikan dua jenis
protein ini dan fungsinya dalam organisma hidup.                                     [4 markah]

19 (a) Bezakan kromosom bacteria daripada kromosom eukariot.                            [8 markah]
   (b) Perihalkan ciri anatomi yang ketara bagi berkas vaskular pada daun jagung.       [7 markah]

20 (a) Perihalkan tiga kelas enzim yang berbeza berdasarkan tindak balas yang dimangkinkan.
                                                                                      [6 markah]
   (b) Dengan memberi dua contoh tindak balas enzim, jelaskan bagaimana kofaktor dan perencat
yang sesuai mempengaruhi aktiviti masing-masing.                                      [9 markah]




964/1


                                                43
44
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)
964 Sukatan Pelajaran  Biologi STPM (Baharu)

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964 Sukatan Pelajaran Biologi STPM (Baharu)

  • 1. STPM/S(E)964 MAJLIS PEPERIKSAAN MALAYSIA (MALAYSIAN EXAMINATIONS COUNCIL) PEPERIKSAAN SIJIL TINGGI PERSEKOLAHAN MALAYSIA (MALAYSIA HIGHER SCHOOL CERTIFICATE EXAMINATION) BIOLOGY Syllabus, Specimen Papers and Specimen Experiment This syllabus applies for the 2012/2013 session and thereafter until further notice.
  • 2. FALSAFAH PENDIDIKAN KEBANGSAAN “Pendidikan di Malaysia adalah satu usaha berterusan ke arah memperkembangkan lagi potensi individu secara menyeluruh dan bersepadu untuk mewujudkan insan yang seimbang dan harmonis dari segi intelek, rohani, emosi, dan jasmani. Usaha ini adalah bagi melahirkan rakyat Malaysia yang berilmu pengetahuan, berakhlak mulia, bertanggungjawab, berketerampilan, dan berkeupayaan mencapai kesejahteraan diri serta memberi sumbangan terhadap keharmonian dan kemakmuran keluarga, masyarakat dan negara.”
  • 3. FOREWORD This revised Biology syllabus is designed to replace the existing syllabus which has been in use since the 2001 STPM examination. This new syllabus will be enforced in 2012 and the first examination will also be held the same year. The revision of the syllabus takes into account the changes made by the Malaysian Examinations Council (MEC) to the existing STPM examination. Through the new system, sixth-form study will be divided into three terms, and candidates will sit for an examination at the end of each term. The new syllabus fulfils the requirements of this new system. The main objective of introducing the new examination system is to enhance the teaching and learning orientation in sixth form so as to be in line with the orientation of teaching and learning in colleges and universities. The revision of the Biology syllabus incorporates current developments in biology studies to be more relevant to the current global developments. Biology is a science that deals with the study of living organisms. It is dynamic and affects every aspect of our daily lives, from food and health, to the responsibilities towards our environment. The cumulative discoveries and developments in biology have tremendously enhanced our understanding and perception towards both the unity and diversity of life. With this understanding, we have become more aware of our interdependence with our rich biodiversity and natural resources. The application of biology together with modern technologies leads to the evolution of biotechnology. Furthermore, the assessment tools of this syllabus consist of written papers and coursework. The written papers evaluate candidates’ knowledge and understanding of the subject, while the coursework provides an opportunity for candidates to trigger their inquisitive biological reasoning. This also enhances their understanding and application of biological sciences and develops the candidates’ soft skills. The syllabus contains topics, teaching periods, learning outcomes, examination format, grade description, and sample questions. The design of this syllabus was undertaken by a committee chaired by Professor Emeritus Dato’ Dr. Latiff bin Mohamad of Universiti Kebangsaan Malaysia. Other committee members consist of university lecturers, representatives from the Curriculum Development Division, Ministry of Education Malaysia, and experienced teachers teaching Biology. On behalf of the Malaysian Examinations Council, I would like to thank the committee for their commitment and invaluable contribution. It is hoped that this syllabus will be a guide for teachers and candidates in the teaching and learning process. OMAR BIN ABU BAKAR Chief Executive Malaysian Examinations Council
  • 4. CONTENTS Syllabus 964 Biology Page Aims 1 Objectives 1 Content First Term: Biological Molecules and Metabolism 2–8 Second Term: Physiology 9 – 15 Third Term: Ecology and Genetics 16 – 21 Practical Syllabus (School-based Assessment of Practical (Paper 4)) 22 Written Practical Test (Paper 5) 23 Scheme of Assessment 24 – 25 Performance Descriptions 26 Reference Books 27 Specimen Paper 1 29 – 43 Specimen Paper 2 45 – 63 Specimen Paper 3 65 – 81 Specimen Experiment Paper 4 83 – 86 Specimen Paper 5 87 – 101
  • 5. SYLLABUS 964 BIOLOGY Aims This syllabus is designed to enhance candidates’ knowledge and understanding of biology and biological issues, to prepare and equip the candidates for their tertiary education, to pursue careers in related fields and to promote continuous awareness of the importance of biology in life. Objectives This syllabus enables candidates to: (a) understand the biological phenomena, principles and theories; (b) evaluate biological information critically and deduce logical conclusion; (c) plan and carry out experiments scientifically and make deductions; (d) develop abilities and skills in handling materials and apparatus correctly and safely, and; (e) cultivate proper attitudes and values on social, technological, and environmental issues in biology. 1
  • 6. FIRST TERM: BIOLOGICAL MOLECULES AND METABOLISM Teaching Topic Learning Outcome Period 1 Biological Molecules 24 1.1 Water 2 Candidates should be able to: (a) describe the chemical properties (solvent, bond angles and hydrogen bond) of water and relate its physiological roles in the organisms; (b) describe the physical properties (polarity, cohesiveness, density, surface tension, specific heat capacity, and latent heat of vaporisation) of water and relate its physiological roles in organisms. 1.2 Carbohydrates 6 Candidates should be able to: (a) classify carbohydrates into monosaccharide, disaccharide and polysaccharide with respect to their physical and chemical properties; (b) classify monosaccharide according to the number of carbon atoms and the functional groups (i) triose e.g. glyceraldehydes, (ii) pentose e.g. ribose and deoxyribose, (iii) hexose e.g. glucose and fructose, (c) illustrate the molecular structure of a monosaccharide and differentiate between the reducing and non-reducing ends; (d) describe the formation of glycosidic bond in disaccharides (maltose and sucrose) and polysaccharides (starch, glycogen and cellulose); (e) relate the structure of disaccharides and polysaccharides to their functions in living organisms. 2
  • 7. Teaching Topic Learning Outcome Period 1.3 Lipids 3 Candidates should be able to: (a) describe the structures, properties and distribution of triglycerides, phospholipids (lecithin) and steroid (cholesterol); (b) state the functions of triglycerides, phospholipids (lecithin) and steroids (cholesterol); (c) differentiate between saturated and unsaturated fatty acids. 1.4 Proteins 6 Candidates should be able to: (a) classify amino acids into four main classes based on their side chains: polar, non-polar, acidic and basic; (b) describe the structure of an amino acid and the formation of peptide bonds in polypeptides; (c) explain the properties of protein (amphoteric, isoelectric point, buffer and colloid); (d) differentiate the various levels of organisation of protein structure (primary, secondary, tertiary and quaternary) and relate the functions of each structure to the organisation of proteins; (e) explain the denaturation and renaturation of protein; (f) classify proteins according to their structures, compositions (simple and conjugated) and functions. 1.5 Nucleic acids 3 Candidates should be able to: (a) describe the structures of nucleotides and the formation of phosphodiester bonds in a polynucleotide; (b) distinguish between DNA and RNA and the three types of RNAs (mRNA, tRNA and rRNA); (c) describe the structure of DNA based on Watson and Crick model. 3
  • 8. Teaching Topic Learning Outcome Period 1.6 Analytical techniques 4 Candidates should be able to: (a) describe the basic principles of paper chromatography in pigment separation, electrophoresis for protein and nucleic acid separation. 2 Structure of Cells and 16 Organelles 2.1 Prokaryotic and 4 Candidates should be able to: eukaryotic cells (a) state the cell theory; (b) compare the structures of prokaryotic and eukaryotic cells; (c) compare typical animal and plant cells as seen under electron microscopes; (d) describe the basic principles of light and electron microscopy. 2.2 Cellular components 6 Candidates should be able to: (a) identify the cellular components of typical plant and animal cells; (b) describe the structures of organelles and state their functions; (c) explain the basic principles of differential centrifugation used to fractionate cellular components (g and S values). 2.3 Specialised cells 6 Candidates should be able to: (a) outline the structures, functions and distributions of unspecialised cells found in plants (meristematic cells); (b) describe the structures, functions and distributions of specialised plant cells found in epidermal, ground and vascular tissue; (c) describe the structures, functions and distributions of specialised animal cells found in connective, nervous, muscular and epithelial tissues, including the formation of endocrine and exocrine glands. 4
  • 9. Teaching Topic Learning Outcome Period 3 Membrane Structure and 8 Transport 3.1 Fluid mosaic model 3 Candidates should be able to: (a) describe the structure of a membrane based on Singer-Nicolson fluid mosaic model; (b) explain the roles of each component of the membrane. 3.2 Movement of 5 Candidates should be able to: substance across membrane (a) explain the processes of passive and active transports, endocytosis and exocytosis; (b) explain the concepts of water potential, solute potential and pressure potential; (c) calculate the water potential of a plant cell in a solution. 4 Enzymes 20 4.1 Catalysis and 3 Candidates should be able to: activation energy (a) explain that enzyme is a globular protein which catalyses a metabolic reaction; (b) explain the mode of action of enzymes at active site involving enzyme-substrate complex and lowering of the activation energy and enzyme specificity. 4.2 Mechanism of action 5 Candidates should be able to: and kinetics (a) illustrate enzyme specificity using induced fit (Koshland) and lock and key (Fischer) models; (b) explain the time course of an enzyme- catalysed reaction by measuring the rate of formation of product(s) or rate of disappearance of substrate(s) as the rate of reaction; (c) deduce the Michaelis-Menten constant (Km) from the Michaelis-Menten and Lineweaver- Burk plots; (d) explain the significance of Km and Vmax; (e) explain the effects of temperature, pH, enzyme concentration and substrate concentration on the rate of an enzyme-catalysed reaction. 5
  • 10. Teaching Topic Learning Outcome Period 4.3 Cofactors 3 Candidates should be able to: (a) explain the roles of cofactors (ion activators, coenzymes and prosthetic groups) in an enzymatic reaction; (b) explain the importance of vitamins and minerals as precursors of coenzymes/cofactors. 4.4 Inhibitors 4 Candidates should be able to: (a) explain the effects of competitive and non- competitive inhibitions on the rate of enzyme activity of reversible inhibition; (b) relate the Lineweaver-Burk plot to the effect of inhibition on Km and Vmax values. 4.5 Classification of 2 Candidates should be able to: enzymes (a) describe enzyme classification according to International Union of Biochemistry (IUB) e.g. oxidoreductase, transferase, hydrolase, lyase, isomerase and ligase. 4.6 Enzyme technology 3 Candidates should be able to: (a) explain the importance and the main techniques of enzyme immobilisation namely adsorption, entrapment and covalent coupling; (b) explain the application of enzyme immobilisation in the development of biosensors. 5 Cellular Respiration 12 5.1 The need for energy in 1 Candidates should be able to: living (a) outline the importance of energy and respiration in living organisms; (b) describe the structure of the energy carriers such as ATP, NADH and FADH2. 5.2 Aerobic respiration 8 Candidates should be able to: (a) describe the various stages of aerobic respiration and its location in the cells; (b) describe glycolysis, and calculate the net energy produced in glycolysis; 6
  • 11. Teaching Topic Learning Outcome Period (c) describe the various steps involved in the Krebs cycle (including the link reaction); (d) explain the formation of NADH, FADH2, GTP and ATP during the Krebs cycle; (e) describe oxidative phosphorylation and chemiosmosis in the electron transport system; (f) explain the role of NADH, FADH2 and ATP synthase in the electron transport chain; (g) calculate and explain the net energy produced in aerobic respiration per molecule of glucose in liver and muscle cells; (h) describe the effects of cyanide and carbon monoxide on respiration; (i) explain how lipid and protein act as alternative energy sources. 5.3 Anaerobic respiration 3 Candidates should be able to: (a) explain the anaerobic respiration in yeast and muscle cells; (b) describe the applications of anaerobic respiration in food industries (bread, tapai, and yogurt). 6 Photosynthesis 16 6.1 Autotroph 3 Candidates should be able to: (a) classify autotroph into photoautotroph and chemoautotroph; (b) describe photosynthetic pigments; (c) explain the absorption spectrum and action spectrum of photosynthetic pigments. 6.2 Light-dependent 3 Candidates should be able to: reactions (a) explain photo-activation of chlorophyll a resulting in photolysis of water; (b) explain the cyclic and non-cyclic photophosphorylation including electron transport system resulting in the production of ATP and NADPH. 7
  • 12. Teaching Topic Learning Outcome Period 6.3 Light-independent 8 Candidates should be able to: reactions (a) describe Calvin cycle; (b) explain photorespiration; (c) describe the anatomical structure of C4 leaf (Krantz anatomy) in comparison to C3 leaf; (d) explain carbon dioxide fixation in C4 plants and Crassulacean Acid Metabolism (CAM) plants; (e) differentiate the metabolism of C3, C4 and CAM plants. 6.4 Limiting factors 2 Candidates should be able to: (a) explain limiting factors of photosynthesis (light intensity, carbon dioxide concentration and temperature); (b) relate the roles of C3, C4 and CAM plants on the increasing carbon dioxide emission and global warming. 8
  • 13. SECOND TERM: PHYSIOLOGY Teaching Topic Learning Outcome Period 7 Gas Exchange 12 7.1 Gaseous exchange in 7 Candidates should be able to: humans (a) outline the structure of human respiratory system, including the microscopic structure of the wall of an alveolus; (b) describe the structure of haemoglobin; (c) explain the transport of oxygen and carbon dioxide in blood; (d) explain the oxygen dissociation curves of haemoglobin, myoglobin and foetal haemoglobin; (e) explain the Bohr effect and relate it to the oxygen dissociation curve. 7.2 Breathing cycle 3 Candidates should be able to: (a) explain the control of breathing mechanism, including the role of chemoreceptor; (b) define tidal volume, vital capacity, total lung capacity, inspiratory reserve volume, expiratory reserve volume and residual volume. 7.3 Gaseous exchange in 2 Candidates should be able to: plants (a) describe the structure and functions of stomata; (b) describe the mechanism of opening and closing of stomata based on potassium ion accumulation hypothesis. 8 Transport in Animals and 16 Plants 8.1 Transport system in 8 Candidates should be able to: mammals (a) describe the structure of a mammalian heart; (b) define systole and diastole, and explain the sequence of events in a cardiac cycle including changes in pressure and volume in aorta, left atrium and left ventricle; (c) describe the initiation and regulation of heart beat; 9
  • 14. Teaching Topic Learning Outcome Period (d) explain hypertension, atherosclerosis, arteriosclerosis and myocardial infarction, and state their causes and preventions; (e) describe the lymphatic system in relation to the blood circulatory system; (f) determine the direction of fluid movement at the arterial and venous ends of the capillaries by calculating the differences between osmotic pressure/solute potential and hydrostatic pressure. 8.2 Transport system in 8 Candidates should be able to: vascular plants (a) explain the uptake of water and mineral ions from the soil by the root hairs involving water potential; (b) describe the apoplast, symplast and vacuolar pathway of water movement through the root tissues; (c) describe the root pressure, cohesion-tension theory and transpiration pull in relation to water movement from the roots to leaves; (d) explain translocation using the mass flow, electro-osmosis, cytoplasmic streaming and peristaltic waves hypotheses; (e) explain the concept of source and sink, and phloem loading and unloading in translocation according to pressure flow hypothesis. 9 Control and Regulation 22 9.1 Nervous system 16 Candidates should be able to: (a) describe the organisation of the nervous system in humans; (b) explain the formation of resting and action potentials; (c) describe the characteristics of nerve impulse; (d) describe the structure of synapse, and explain the role of neurotransmitters (acetylcholine and norepinephrine); (e) explain and compare the mechanisms of impulse transmission along the axon and across the synapse; 10
  • 15. Teaching Topic Learning Outcome Period (f) describe the structure of neuromuscular junction and sarcomere; (g) explain the role of sarcoplasmic reticulum, calcium ions, myofibril and T tubules in muscle contraction; (h) explain the mechanism of muscle contraction according to the sliding filament hypothesis; (i) compare the sympathetic and parasympathetic nervous systems; (j) explain the mechanisms of drug action on nervous system and neuromuscular junction (cocaine and curare). 9.2 Hormones 6 Candidates should be able to: (a) explain the mechanisms of action of steroid hormone and non-steroid hormones; (b) explain the roles of plant hormones in growth and development; (c) explain the mechanism of phytochrome action and their roles in photoperiodism and flowering; (d) outline the application of plant growth regulators (synthetic auxin, synthetic gibberellins, and synthetic ethylene) in agriculture. 10 Reproduction, 13 Development and Growth 10.1 Sexual reproduction in 6 Candidates should be able to: humans (a) outline spermatogenesis and oogenesis; (b) describe the passage and development of sperms from the testis to the oviduct for fertilisation; (c) describe the process of fertilisation and implantation; (d) describe the roles of hormones in menstrual cycle and pregnancy; (e) describe briefly the stages in embryonic development; 11
  • 16. Teaching Topic Learning Outcome Period (f) explain the roles of placenta, chorion, amniotic fluid and allantois in foetal development; (g) explain the process of parturition. 10.2 Sexual reproduction in 2 Candidates should be able to: flowering plants (a) outline double fertilisation; (b) describe the embryonic development in seed and formation of fruit. 10.3 Seed germination 1 Candidates should be able to: (a) explain the mobilisation of nutrients after imbibition in seed germination; (b) state the external factors affecting germination. 10.4 Growth curves and 4 Candidates should be able to: patterns of growth (a) explain the types of growth curves (absolute growth curve, absolute growth rate curve and relative growth rate curve); (b) explain with examples the patterns of growth (limited growth in humans, unlimited growth in perennial plant, allometric growth in humans, isometric growth in fish and intermittent growth in insect); (c) explain the processes of ecdysis and metamorphosis in insects, and relate the role of hormones (neurosecretory hormone, juvenile hormone and ecdysone) in these processes. 11 Homeostasis 10 11.1 Importance of 2 Candidates should be able to: homeostasis (a) explain the importance of homeostasis; (b) describe the homeostatic control system in mammals; (c) explain the physiological and behavioural control in thermoregulation of endotherms. 12
  • 17. Teaching Topic Learning Outcome Period 11.2 Liver 4 Candidates should be able to: (a) describe the structure of liver, and explain the roles of its components; (b) describe carbohydrate metabolism in the liver (glycogenesis, glycogenolysis, gluconeogenesis); (c) describe protein metabolism (transamination, deamination and urea formation) in the liver. 11.3 Osmoregulation in 3 Candidates should be able to: mammals (a) explain the process of ultrafiltration, reabsorption and secretion in the formation of urine; (b) explain the role of ADH and aldosterone, and the related hormones in regulating water, sodium and potassium ions of urine; (c) explain the regulation of pH of tissue fluid. 11.4 Osmoregulation in 1 Candidates should be able to: plants (a) describe the role of stomata in regulation of water loss, and explain the importance of transpiration; (b) describe the various types of plant adaptations to prevent water loss (halophytes and xerophytes). 12 Immunity 10 12.1 Immune system 3 Candidates should be able to: (a) describe human lymphatic system, and explain its function in relation to immunity; (b) describe antibody (structure and function), antigen, epitope, and the development of B and T cells; (c) describe the roles of macrophages, B cells and T cells. 13
  • 18. Teaching Topic Learning Outcome Period 12.2 Development of 3 Candidates should be able to: immunity (a) explain cell-mediated and humoral immune responses; (b) outline the antigen-antibody reactions (precipitation, agglutination, neutralisation, complement fixation). 12.3 Concept of self and 3 Candidates should be able to: non-self (a) explain the concept of self and non-self and relate this to tissue rejection in organ transplant; (b) explain the mechanism of immune suppression (HIV infection). 12.4 Immune disorder 1 Candidates should be able to: (a) describe autoimmune disorder (Systemic Lupus Erythematosus (SLE)). 13 Infectious Diseases 13 13.1 Infectious disease 1 Candidates should be able to: (a) explain what is meant by an infection and an infectious disease; (b) outline the types of infectious agents. 13.2 Dengue 3 Candidates should be able to: (a) describe the causes and symptoms of dengue; (b) explain the transmission of dengue; (c) discuss the roles of social, economical and biological factors in the prevention of dengue. 13.3 Cholera 3 Candidates should be able to: (a) describe the causes and symptoms of cholera; (b) explain the transmission of cholera; (c) discuss the roles of social, economical and biological factors in the prevention of cholera. 14
  • 19. Teaching Topic Learning Outcome Period 13.4 Tuberculosis (TB) 3 Candidates should be able to: (a) describe the causes and symptoms of tuberculosis (TB); (b) explain the transmission of TB; (c) discuss the roles of social, economical and biological factors in the prevention of TB. 13.5 Malaria 3 Candidates should be able to: (a) describe the causes and symptoms of malaria; (b) explain the transmission of malaria; (c) discuss the roles of social, economical and biological factors in the prevention of malaria. 15
  • 20. THIRD TERM: ECOLOGY AND GENETICS Teaching Topic Learning Outcome Period 14 Taxonomy and 14 Biodiversity 14.1 Taxonomy 2 Candidates should be able to: (a) explain the importance of taxonomy in biological sciences; (b) explain the concept of species, and relate how a species is classified into higher categories in a taxonomic hierarchy. 14.2 Diversity of organisms 6 Candidates should be able to: (a) describe the morphological characteristics of the following phyla in the respective kingdoms: Protoctista (Chlorophyta and Zoomastigina), Fungi (Zygomycota), Plantae (Bryophyta, Filicinophyta, Coniferophyta and Angiospermophyta) and Animalia (Porifera, Cnidaria, Platyhelminthes, Mollusca, Arthropoda and Chordata). 14.3 Biodiversity in 2 Candidates should be able to: Malaysia (a) describe the different levels and examples of biodiversity in Malaysia, namely ecosystem or community diversity, species or taxonomic diversity and genetic diversity; (b) explain the importance of biodiversity in Malaysia. 14.4 Threats to biodiversity 2 Candidates should be able to: (a) explain the natural and man-made factors that threaten biodiversity in Malaysia; (b) explain the steps and efforts taken by various agencies and organisations to address the threats. 14.5 Conservation of 2 Candidates should be able to: biodiversity (a) describe the various measures taken to conserve the different levels of biodiversity including in situ and ex situ conservation in Malaysia. 16
  • 21. Teaching Topic Learning Outcome Period 15 Ecology 22 15.1 Levels of ecological 3 Candidates should be able to: organisation (a) explain the concept of hierarchy in an ecosystem and the interaction between the biotic and abiotic components. 15.2 Biogeochemical cycles 3 Candidates should be able to: (a) describe the biogeochemical cycles (carbon, phosphorus and sulphur), and explain their importance. 15.3 Energy flow 3 Candidates should be able to: (a) describe the energy flow and the efficiency of energy transfer in terrestrial ecosystem (tropical rain forest) and aquatic ecosystem (lake). 15.4 Population ecology 6 Candidates should be able to: (a) explain population growth (S and J growth curves), biotic potential, natality, mortality, migration and survivorship; (b) explain the characteristics of populations that show Type I, Type II and Type III survivorship curves, and K-strategies and r-strategies. 15.5 Carrying capacity 3 Candidates should be able to: (a) explain what is meant by carrying capacity and sustainable development; (b) explain the factors limiting the population size and distribution. 15.6 Quantitative ecology 4 Candidates should be able to: (a) describe the use of quadrat and line transect sampling methods and explain the advantages and disadvantages of using these methods; (b) calculate the various sampling parameters (frequency, density, cover and their absolute and relative estimations) and estimate the population size of organisms; (c) explain the pattern of distribution of organisms in an ecosystem. 17
  • 22. Teaching Topic Learning Outcome Period 16 Selection and Speciation 10 16.1 Natural and artificial 6 Candidates should be able to: selection (a) describe continuous and discontinuous variations in relation to selection and speciation; (b) explain the modes of natural selection (stabilising, directional and disruptive) and their consequences; (c) describe with examples, sexual selection and polymorphism; (d) explain the importance of artificial selection (gene bank, germplasm bank and sperm bank). 16.2 Speciation 4 Candidates should be able to: (a) explain the processes of isolation, genetic drift, hybridisation and adaptive radiation; (b) explain the importance of speciation in relation to evolution. 17 Inheritance and Genetic 34 Control 17.1 Types of genetic 5 Candidates should be able to: crosses and breeding (a) explain the Mendelian inheritance pertaining system to the phenotypic and genotypic ratios; (b) describe the types of crosses (test cross, backcross, reciprocal cross and selfing) and explain their importance; (c) describe pure breeding, outbreeding, inbreeding, selective breeding, and explain their importance. 17.2 Non-Mendelian 7 Candidates should be able to: inheritance (a) explain (i) incomplete dominance (flower colour in snapdragon), (ii) codominance (MN blood group in humans), (iii) multiple alleles (ABO blood group in humans), and calculate the genotypic and phenotypic ratios; 18
  • 23. Teaching Topic Learning Outcome Period (b) explain lethal genes (sickle-cell in human/coat colur in mice/chlorophyll production in maize), polygenes (height in humans), linked and sex-linked genes (Drosophila eye colour and haemophilia in humans), and epistasis (coat colour in dog and capsule shape in shepherd’s purse plant); (c) explain the pedigree analysis. 17.3 Genetic mapping 2 Candidates should be able to: (a) explain crossing over and distinguish between parental and recombinant genotypes and phenotypes; (b) calculate the distance between two loci, and determine the relative position of a gene on a chromosome based on percentage of crossing- over in Drosophila. 17.4 Population genetics 5 Candidates should be able to: (a) describe the concept of gene pool, gene/allele frequency and genotype frequency; (b) explain Hardy-Weinberg equilibrium (p2 + 2pq +q2 = 1 and p + q = 1), and calculate the gene/allele and genotype frequencies; (c) explain the conditions for Hardy-Weinberg equilibrium to be valid; (d) describe changes in genotype frequencies in relation to evolution. 17.5 DNA replication 4 Candidates should be able to: (a) explain the experiments to prove DNA is the genetic material (Avery, MacLeod and McCarty experiment and Hershey and Chase experiment); (b) explain the three models of DNA replication, and interpret the experiment of Meselson and Stahl to prove the semi-conservative model of DNA replication; (c) explain the mechanism of DNA replication, and the role of the enzymes involved. 19
  • 24. Teaching Topic Learning Outcome Period 17.6 Gene expression 4 Candidates should be able to: (a) explain the experiment of Beadle and Tatum which leads to the establishment of one-gene- one-polypeptide hypothesis; (b) interpret the genetic code table, and identify the appropriate anti-codon; (c) explain the characteristics of genetic code; (d) describe transcription and translation. 17.7 Regulation of gene 2 Candidates should be able to: expression (a) define repressor, inducer, negative control in lac operon and constitutive enzyme; (b) describe the components of lac operon, and explain its mechanism. 17.8 Mutation 5 Candidates should be able to: (a) describe the different types of gene mutation with examples of its consequences (substitution – sickle-cell anaemia, insertion/addition – frameshift mutation, deletion – frameshift mutation and thalassaemia major and inversion); (b) differentiate missense, nonsense and silent/ neutral mutations; (c) describe the four structural changes in chromosomes (duplication, deletion, inversion and translocation); (d) describe the changes in chromosome number, including the definition of non-disjunction; (e) describe the consequences of non-disjunction in relation to meiosis; (f) explain and give examples of different types of aneuploidy (monosomy and trisomy); (g) explain and give examples of different types of euploidy: diploid and polyploid, including autopolyploidy and allopolyploidy. 20
  • 25. Teaching Topic Learning Outcome Period 18 Gene Technology 8 18.1 Recombinant DNA 8 Candidates should be able to: technology (a) explain recombinant DNA technology/genetic engineering; (b) differentiate between genomic and cDNA cloning and genomic and cDNA libraries; (c) describe the vectors used in cloning and their properties; (d) describe the restriction enzyme (EcoR1 and SmaI), including its nomenclature, recognition site (palindrome), importance and the types of ends generated; (e) explain reverse transcription, insertion, ligation, transformation/transduction, amplification and screening; (f) describe the steps involved in genomic and cDNA cloning, including the enzymes involved, and explain human insulin production in E. coli as an example. 19 Biotechnology 8 19.1 Roles of 1 Candidates should be able to: biotechnology (a) define biotechnology; (b) outline the roles of biotechnology in our life. 19.2 Applications of 7 Candidates should be able to: biotechnology (a) describe the application of biotechnology in food and beverages production (fermentation and vitamin-enriched eggs); (b) describe the application of biotechnology in agriculture (hybrid rice, herbicide resistant plants and transgenic fish); (c) describe the application of biotechnology in medicine (human growth hormone, human insulin and gene therapy) and forensic (DNA finger printing); (d) describe the application of biotechnology in public health (genetic screening, diagnostic kits and oil-decomposing bacteria). 21
  • 26. The Practical Syllabus School-based Assessment of Practical (Paper 4) School-based assessment of practical work will be carried out throughout the form six school terms for candidates from government schools and private schools which are approved by the MEC to carry out the school-based assessment of practical work. MEC will determine 15 compulsory experiments to be carried out by the candidates and to be assessed by the subject teachers in schools in the respective terms. Details of the title, objective, learning outcome, introduction, material, apparatus and procedure of each of the experiments will be specified in the Teacher’s and Student’s Manual for Practical Biology which can be downloaded from MEC Portal (http://www.mpm.edu.my) during the first term of form six by the subject teacher. Candidates should be supplied with a work scheme before the day of the compulsory experiment so as to enable them to plan their practical work. Each experiment is expected to last one school double period. Assessment of the practical work is done by the subject teachers during the practical sessions and also based on the practical reports. The assessment should comply with the assessment guidelines prepared by MEC. A repeating candidate may use the total mark obtained in the coursework for two subsequent examinations. Requests to carry forward the moderated coursework mark should be made during the registration of the examination. During the practical session, candidates should be able to: (a) make observations on living, preserved specimens and models, and record them accurately in writing or by using clear drawings or diagrams, (b) identify morphological features of specimens as a basis for their classification, (c) carry out dissection, display and illustrate the result, (d) stain and mount specimens for microscopic examination, (e) identify and illustrate the specimens observed under light microscope, (f) carry out biochemical, physiological and genetic experiments, (g) carry out sampling for ecological studies, (h) record the results systematically, interpret the data scientifically and make conclusion. 22
  • 27. Written Practical Test (Paper 5) The main objective of written practical test is to assess the candidates’ understanding of practical procedures in the laboratory. The following candidates are required to register for this paper: (a) individual private candidates, (b) candidates from private schools which have no permission to carry out the school-based assessment of practical work, (c) candidates who repeat upper six (in government or private schools), (d) candidates who do not attend classes of lower six and upper six in two consecutive years (in government or private schools), (e) candidates who take Biology other than the package offered by schools. Three structured questions related to practical work will be set. MEC will not be strictly bound by the syllabus in setting questions. Where appropriate, candidates will be given sufficient information to enable them to answer the questions. Only knowledge of theory within the syllabus and knowledge of usual laboratory practical procedures will be expected. Candidates should have sufficient knowledge on: (a) structure of specimens and record them accurately in writing or illustration, (b) morphological and anatomical features and relate to functions of specimens as a basis for their classification, (c) dissection of specimens and illustration of the result (rat), (d) staining and mounting specimens for microscopic examination, (e) identification and illustration of the specimens as observed under microscope, (f) biochemical, physiological and genetic experiments, (g) sampling for ecological studies, (h) interpreting, evaluating, drawing conclusion from experimental data, (i) using theories to explain the results of experiments and performing simple calculations based on experiments. 23
  • 28. Scheme of Assessment Term of Paper Code Mark Theme/Title Type of Test Duration Administration Study and Name (Weighting) First 964/1 Biological Written test 60 Term Biology Molecules (26.67%) Paper 1 and Section A 15 Metabolism 15 compulsory multiple-choice questions to be answered. Section B 15 Central 2 compulsory short 1½ hours assessment structured questions to be answered. Section C 30 2 out of 3 essay questions to be answered. All questions are based on topics 1 to 6. Second 964/2 Physiology Written test 60 Term Biology (26.67%) Paper 2 Section A 15 15 compulsory multiple-choice questions to be answered. Section B 15 2 compulsory short Central 1½ hours structured questions to assessment be answered. Section C 30 2 out of 3 essay questions to be answered. All questions are based on topics 7 to 13. 24
  • 29. Term of Paper Code Mark Theme/Title Type of Test Duration Administration Study and Name (Weighting) Third 964/3 Ecology and Written test 60 Term Biology Genetics (26.67%) Paper 3 Section A 15 15 compulsory multiple-choice questions to be answered. Section B 15 2 compulsory short Central 1½ hours structured questions to assessment be answered. Section C 30 2 out of 3 essay questions to be answered. All questions are based on topics 14 to 19. 964/5 Written Practical 45 Biology Test (20%) Paper 5 Central 3 structured questions 1½ hours assessment with diagram/graph/ table to be answered. First, 964/4 Biology School-based 225 Second Biology Practical Assessment of (20%) and Through- Paper 4 Practical Third out the School-based Terms 15 compulsory three assessment experiments to be terms carried out. 25
  • 30. Performance Descriptions A Grade A candidate is likely able to: (a) recall almost all the content of required areas of biological syllabus, and relate the content with the question; (b) decide on, organise and present information to explain the concept, theories and depth of biological knowledge in an ordered and logical manner; (c) plan and carry out investigations, solve problems based on a sound biological knowledge; (d) interpret and analyse complex data presented, and relate the biological knowledge and understanding of content by using correct formulae, terminologies, scientific units and approach; (e) integrate various concepts and findings accurately, and formulate logical conclusion; (f) apply and justify knowledge and understanding successfully to unfamiliar contexts and data. A Grade C candidate is likely able to: (a) recall some of the content of the required areas of biological syllabus, and relate the content with the question moderately; (b) decide on, organise and present information to describe the concept, theories and depth of biological knowledge; (c) plan and carry out investigations, solve problems based on a mediocre biological knowledge; (d) interpret and analyse simple and straight forward data presented, and relate the biological knowledge and understanding of content by using some formulae, terminologies and scientific units; (e) integrate some concepts and findings, and formulate simple conclusion; (f) apply knowledge with moderate understanding to unfamiliar contexts and data. 26
  • 31. Reference Books Teachers and candidates may use books specially written for the STPM examination and other reference books such as those listed below. 1. Alters, S. and Alters, B., 2006. Biology: Understanding Life. New Jersey: John Wiley & Sons, Inc. 2. Audesirk, T., Audesirk, G. and Byers, B. E., 2001. Biology: Life on Earth. 6th edition. New Jersey: Prentice Hall. 3. Campbell, M. K., Farrell, S. O., 2006. Biochemistry. Belmont: Thomson Brooks/Cole. 4. Campbell, N. A. and Reece, N. A., 2005. Biology. 7th edition. San Francisco: Benjamin/ Cummings. 5. Clegg, C. J. and Mackean, D. G., 2000. Advanced Biology: Principles and Applications. 2nd edition. London: John Murray. 6. Kent, M., 2000. Advanced Biology. Oxford: Oxford University Press. 7. Klug, W. S., Cummings, M.R. and Spencer, C. A., 2005. Concepts of Genetics. 8th edition. New Jersey: Pearson Prentice Hall. 8. Klug, W. S. and Cummings, M. R., 2005. Essentials of Genetics. 5th edition. New Jersey: Pearson Prentice Hall. 9. Mader, S., 2004. Biology. 8th edition. New York: McGraw-Hill. 10. Raven, P. H., et. al. 2005. Biology. 7th edition. New York: McGraw-Hill. 11. Russel, P. J., et. al. 2008. Biology: The Dynamics Science. 1st edition. Belmont: Thompson Brooks/Cole. 12. Smith, R. L. and Smith, T. M., 2003. Elements of Ecology. 5th edition. San Francisco: Benjamin Cummings. 13. Solomon, E. P., Berg, L. R. and Martin, D. W., 2005. Biology. 7th edition. Belmont: Thompson Brooks/Cole. 14. Taylor, D. J., Green, N. P. O. and Stout, G. W., 2003. Biological Science 1: Organisms, Energy and Environment. 3rd edition. Cambridge: Cambridge University Press. 15. Taylor, D. J., Green, N. P. O. and Stout, G. W., 2003. Biological Science 2: Systems, Maintenance and Change. 3rd editon. Cambridge: Cambridge University Press. 16. Toole, G. and Toole, S., 1999. Understanding Biology for Advanced Level. 4th edition. United Kingdom: Nelson Thornes. 27
  • 32. 28
  • 33. Identity card number:………………………….. Centre number/index number:………………………. (Nombor kad pengenalan) (Nombor pusat/angka giliran) SPECIMEN PAPER 964/1 STPM BIOLOGY (BIOLOGI) PAPER 1 (KERTAS 1) One and a half hours (Satu jam setengah) MAJLIS PEPERIKSAAN MALAYSIA (MALAYSIAN EXAMINATIONS COUNCIL) SIJIL TINGGI PERSEKOLAHAN MALAYSIA (MALAYSIA HIGHER SCHOOL CERTIFICATE) Instructions to candidates: DO NOT OPEN THIS QUESTION PAPER UNTIL YOU ARE TOLD TO DO SO. Answer all questions in Section A. Marks will not be deducted for wrong answers. For each question, four suggested answers are given. Choose the correct answer and circle the answer. Answer all questions in Section B. Write your answers in the spaces provided. Answer any two questions in Section C. All essential working should be shown. For numerical answers, unit should be quoted wherever appropriate. Begin each answer on a fresh sheet of paper and arrange your answers in numerical order. Arahan kepada calon: JANGAN BUKA KERTAS SOALAN INI SEHINGGA ANDA DIBENARKAN BERBUAT DEMIKIAN. Jawab semua soalan dalam Bahagian A. Markah tidak akan ditolak bagi jawapan yang salah. Bagi setiap soalan, empat cadangan jawapan diberikan. Pilih jawapan yang betul dan buat bulatan pada jawapan tersebut. Jawab semua soalan dalam Bahagian B. Tulis jawapan anda di ruang yang diberikan. Jawab mana-mana dua soalan dalam Bahagian C. Semua jalan kerja yang sesuai hendaklah ditunjukkan. Bagi jawapan berangka, unit hendaklah dinyatakan di mana-mana yang sesuai. Mulakan setiap jawapan pada helaian kertas jawapan yang baharu dan susun jawapan anda mengikut tertib berangka. This question paper consists of printed pages and blank page. (Kertas soalan ini terdiri daripada halaman bercetak dan halaman kosong.) © Majlis Peperiksaan Malaysia STPM 964/1 29
  • 34. Section A [15 marks] Answer all questions in this section. 1 Which property of water is important to living organisms? A Polar molecule B Non polar molecule C Low specific heat capacity D Low latent heat of vaporisation 2 Which monosaccharide, example and its description correspond? Monosaccharide Example Description A Pentose Ribose A coenzyme involved in the transfer of hydrogen during glycolysis B Pentose Ribulose A source of NADPH production C Triose Glyceraldehyde An important intermediate substance in glycolysis D Triose Dihydroxyacetone A monomer of cellulose 3 Which is not true of lipid? A It is soluble in water. B It is a solvent for cholesterol. C It is soluble in organic solvents. D It is an ester formed from the reaction of fat and alcohol. 4 Which amino acid is the first to be carried by tRNA to ribosome during translation? A Leucine B Tyrosine C Tryptophan D Methionine 5 Which organelle gives rise to the membranes of a cell? A Vacuole B Nucleus C Lysosome D Smooth endoplasmic reticulum 964/1 30
  • 35. Bahagian A [15 markah] Jawab semua soalan dalam bahagian ini. 1 Sifat air yang manakah yang penting kepada organism hidup? A Molekul berkutub B Molekul tak berkutub C Muatan haba tentu yang rendah D Haba pendam pengewapan yang rendah 2 Monosakarida, contohnya, dan penerangan yang manakah yang berpadanan? Monosakarida Contoh Penerangan A Pentosa Ribosa Satu koenzim yang terlibat dalam pemindahan hydrogen semasa glikolisis B Pentosa Ribulosa Sumber penghasilan NADPH C Triosa Gliseraldehid Bahan perantara penting dalam glikolisis D Triosa Dihidroksiaseton Monomer selulosa 3 Yang manakah yang tidak benar tentang lipid? A Terlarut dalam air. B Pelarut bagi kolesterol. C Terlarut dalam pelarut organik. D Satu ester yang terbentuk daripada tindak balas lemak dan alkohol. 4 Asid amino yang manakah yang pertama dibawa oleh tRNA ke ribosom semasa translasi? A Leusina B Tirosina C Triptofan D Metionina 5 Organel yang manakah yang membentuk membran sel? A Vakuol B Nukleus C Lisosom D Jalinan endoplasma licin 964/1 31
  • 36. 6 Which statement is not true of xylem? A Xylem cells contain pits. B Matured xylem cells are dead cells. C Companion cells provide nutrients to the xylem. D Xylem consists of vessel elements and tracheids. 7 The table below shows the components of a cell membrane and their functions. Component of a cell Functions membrane I Oligosaccharide p Acts as a receptor to a certain molecule such as hormone II Lecithin q As an indicator so that the cells can recognise each other III Protein r Gives the semi-permeable and selective characteristics to the membrane IV Cholesterol s Maintains the fluid characteristics of the membrane Which components of cell membrane and functions correspond? I II III IV A p q r s B q r p s C r p s q D s r q p 8 Which factor reduces enzyme activity? A Extreme pH B Heavy metal C Competitive inhibitor D Very high temperature 964/1 32
  • 37. 6 Penyataan yang manakah yang tidak benar tentang xilem? A Sel xilem mengandungi pit. B Sel xylem yang matang ialah sel mati. C Sel rakan membekalkan nutrien kepada xilem. D Xilem terdiri daripada unsur salur dan trakeid. 7 Jadual di bawah menunjukkan komponen-komponen satu membran sel dan fungsinya. Komponen Fungsi membran sel I Oligosakarida p Bertindak sebagai reseptor kepada molekul tertentu seperti hormon II Lesitin q Sebagai penanda supaya sel boleh mengecam antara satu sama lain III Protein r Memberi sifat separa telap dan sifat memilih pada membran IV Kolesterol s Mengekalkan ciri bendalir bagi membran Yang manakah yang betul bagi komponen membran sel di atas dan fungsi masing-masing? I II III IV A p q r s B q r p s C r p s q D s r q p 8 Faktor yang manakah yang mengurangkan aktiviti enzim? A pH yang melampau B Logam berat C Perencat persaingan D Suhu yang sangat tinggi 964/1 33
  • 38. 9 Which graph shows the possible changes in the rate of a reaction catalysed by an enzyme in the presence of a competitive inhibitor? 1/V Without 1/V With inhibitor inhibitor With Without inhibitor inhibitor 1/[S] 1/[S] A B 1/V Without 1/V inhibitor With With inhibitor inhibitor Without inhibitor 1/[S] 1/[S] C D 10 Which statement describes the advantage of immobilised enzyme? A The efficiency of enzyme increases. B The enzyme can be easily retrieved but cannot be reused. C The end product does not contain the enzyme and easily retrieved. D The characteristics of enzyme can be changed in order to increase its surface area. 11 What is the fate of all carbons from a glucose molecule after it is completely oxidised? A Pyruvate B Carbohydrate C Carbon dioxide D Acetyl coenzyme A 12 In a condition without oxygen, pyruvate in plants will A be converted into lactate B be converted into acetaldehyde C enter the Krebs cycle immediately D be converted back into phosphoglyceraldehyde 964/1 34
  • 39. 9 Graf yang manakah yang menunjukkan kemungkinan perubahan kadar tindak balas yang dimangkinkan oleh enzim dalam kehadiran perencat persaingan? 1/V Tanpa 1/V Dengan perencat perencat Dengan perencat Tanpa perencat 1/[S] 1/[S] A B 1/V Tanpa 1/V perencat Dengan Dengan perencat perencat Tanpa perencat 1/[S] 1/[S] C D 10 Penyataan yang manakah yang memerihalkan kelebihan enzim pentakmobilan? A Keefisienan enzim bertambah. B Enzim boleh didapatkan dengan mudah tetapi tidak boleh digunakan semula. C Produk akhir tidak mengandungi enzim dan mudah didapatkan. D Ciri enzim boleh diubah bagi meningkatkan luas permukaan. 11 Apakah yang terjadi kepada semua karbon dari molekul glukosa selepas dioksidakan dengan lengkap? A Piruvat B Karbohidrat C Karbon dioksida D Asetil koenzim A 12 Dalam keadaan ketiadaan oksigen, piruvat dalam tumbuhan akan A ditukarkan kepada laktat B ditukarkan kepada asetildehid C masuk ke kitar Krebs dengan segera D ditukar semula kepada fosfogliseraldehid 964/1 35
  • 40. 13 In cyclic photophosphorylation, the excited electron which returns to its ground state will emit A light B microwave C gamma ray D ultraviolet radiation 14 Which statement describes the characteristic of bundle sheath cell of C4 plants? A It has small grana. B It contains a few starch granules. C The activity of photosystem I is high. D Carbon dioxide is assimilated by PEP carboxylase. 15 The diagram below shows non-cyclic photophosphorylation during the light reaction of photosynthesis. Which are represented by X, Y and Z ? X Y Z A Ferredoxin Plastoquinone Plastocyanin B Ferredoxin Plastocyanin Plastoquinone C Plastocyanin Plastoquinone Ferredoxin D Plastoquinone Plastocyanin Ferredoxin 964/1 36
  • 41. 13 Dalam pemfotofostorilan berkitar, elektron teruja yang kembali ke keadaan asas akan membebaskan A cahaya B gelombang mikro C sinar gamma D sinaran ultra lembayung 14 Penyataan yang manakah yang memerihalkan ciri sel berkas tumbuhan C4? A Mempunyai grana yang kecil. B Mengandungi beberapa granul kanji. C Aktiviti fotosistem I adalah tinggi. D Karbon dioksida diasimilasi oleh PEP karboksilase. 15 Gambar rajah di bawah menunjukkan pemfotofosforilan bukan kitar semasa tindak balas cahaya fotosintesis. Penerima Penerima pertama pertama Kompleks sitokrom NADP+ reduktase Cahaya Cahaya Yang manakah yang diwakili oleh X, Y, dan Z ? X Y Z A Ferredoksin Plastokuinon Plastosianin B Ferredoksin Plastosianin Plastokuinon C Plastosianin Plastokuinon Ferredoksin D Plastokuinon Plastosianin Ferredoksin 964/1 37
  • 42. Section B [15 marks] Answer all questions in this section. 16 A quantity of cells taken from a type of tissue were homogenised and their organelles were separated by ultracentrifugation. The diagrams below show three types of organelles obtained. R P Q Organelle X Organelle Y Organelle Z (a) State where these cells may have been taken from. [1 mark] ……………………………………………………………………………………………........................ (b) Arrange the order of sedimentation of organelles X, Y and Z. Explain your answer. [2 marks] ……………………………………………………………………………………………........................ ……………………………………………………………………………………………........................ (c) From the above diagrams, name the structure labelled P, Q and R. [3 marks] P: ………………………………………………………………………………………………………... Q: ………………………………………………………………………………………………………... R: ………………………………………………………………………………………………………... (d) Give one function of organelle Y. [1 mark] ……………………………………………………………………………………………........................ (e) Give one common function of organelles X and Z. [1 mark] ……………………………………………………………………………………………........................ 964/1 38
  • 43. Bahagian B [15 markah] Jawab semua soalan dalam bahagian ini. 16 Sejumlah sel yang diambil daripada tisu telah dihomogenkan dan kandungan organelnya diasingkan melalui ultrapengemparan. Gambar rajah di bawah menunjukkan tiga jenis organel yang diperoleh. R P Q Organel X Organel Y Organel Z (a) Nyatakan dari manakah sel-sel ini mungkin telah diperoleh. [1 markah] ……………………………………………………………………………………………........................ (b) Susunkan turutan pengenapan bagi organel X, Y, dan Z. Jelaskan jawapan anda. [2 markah] ……………………………………………………………………………………………........................ ……………………………………………………………………………………………........................ (c) Daripada gambar rajah di atas, namakan struktur yang berlabel P, Q, dan R. [3 markah] P: ………………………………………………………………………………………………………... Q: ………………………………………………………………………………………………………... R: ………………………………………………………………………………………………………... (d) Berikan satu fungsi organel Y. [1 markah] ……………………………………………………………………………………………........................ (e) Berikan satu fungsi sepunya bagi organel X dan Z. [1 markah] ……………………………………………………………………………………………........................ 964/1 39
  • 44. 17 The diagram below shows photophosphorylation in light reaction for plants. Oxidation- reduction potential (Relative energy level) (a) Name the photosystems labeled Q and R, and give the wavelengths of their appropriate reaction centres. [4 marks] Name of photosystem Wavelength Q : …………………… ……………… R: ……………………. ……………… (b) What are the forms of energy represented by S and T? [2 marks] ……………………………………………………………………………………………........................ ……………………………………………………………………………………………........................ (c) Name the process that occurs at Q. [1 mark] ……………………………………………………………………………………………........................ 964/1 40
  • 45. 17 Gambar rajah di bawah menunjukkan pemfotofosforilan dalam tindak balas cahaya bagi tumbuhan. Keupayaan pengoksidaan penurunan (Aras tenaga Penerima elektron relatif) Penerima elektron (a) Namakan fotosistem yang berlabel Q dan R, dan berikan panjang gelombang bagi pusat tindak balas yang sewajarnya. [4 markah] Nama fotosistem Panjang gelombang Q : …………………… ……………………….. R: ……………………. ……………………… (b) Apakan bentuk tenaga yang diwakili oleh S dan T? [2 markah] ……………………………………………………………………………………………........................ (c) Namakan proses yang berlaku di Q. [1 markah] ……………………………………………………………………………………………........................ 964/1 41
  • 46. Section C [30 marks] Answer any two questions in this section. 18 (a) Describe the primary, secondary and tertiary structures of proteins. State the importance of these structures which are related to the properties of proteins. [11 marks] (b) Based on their functions, proteins can be classified into several types. Give two types of these proteins and their functions in living organisms. [4 marks] 19 (a) Distinguish a bacteria chromosome from a eukaryotic chromosome. [8 marks] (b) Describe the distinctive anatomical features of vascular bundles in maize leaves. [7 marks] 20 (a) Explain three different classes of enzymes based on the reactions that they catalyse. [6 marks] (b) By giving two examples of enzymatic reactions, explain how the appropriate cofactor and inhibitor affect their activities. [9 marks] 964/1 42
  • 47. Bahagian C [30 markah] Jawab mana-mana dua soalan daripada bahagian ini. 18 (a) Perihalkan struktur primer, sekunder, dan tertier bagi protein. Nyatakan kepentingan struktur ini yang berkaitan dengan sifat protein. [11 markah] (b) Berdasarkan fungsinya, protein boleh dikelaskan kepada beberapa jenis. Berikan dua jenis protein ini dan fungsinya dalam organisma hidup. [4 markah] 19 (a) Bezakan kromosom bacteria daripada kromosom eukariot. [8 markah] (b) Perihalkan ciri anatomi yang ketara bagi berkas vaskular pada daun jagung. [7 markah] 20 (a) Perihalkan tiga kelas enzim yang berbeza berdasarkan tindak balas yang dimangkinkan. [6 markah] (b) Dengan memberi dua contoh tindak balas enzim, jelaskan bagaimana kofaktor dan perencat yang sesuai mempengaruhi aktiviti masing-masing. [9 markah] 964/1 43
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